ales prokop - Academia.edu (original) (raw)

Papers by ales prokop

Research paper thumbnail of NanoLiterBioReactor: Long-Term Mammalian Cell Culture at Nanofabricated Scale

Biomedical Microdevices, 2004

Research paper thumbnail of System and Methods for Discriminating an Agent

Research paper thumbnail of Capillary perfused bioreactors with multiple chambers

A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present in... more A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the ...

Research paper thumbnail of Bioartificial organs II: technology, medicine & materials

Journal of Vascular Surgery, 2000

together 94 participants that represented medicine, biology, material science, and engineering to... more together 94 participants that represented medicine, biology, material science, and engineering to spend 5 days debating the subject. This book, based on the proceedings of this meeting, represents volume 875 of the Annals of the New York Academy of Sciences. It is edited by the conference organizers. The book is more than 400 pages and organized in seven parts, containing a total of 35 chapters. The first part is written by the main editor, and it provides an overview of the risks and requirements of bioartificial organs. Part II, "Novel Materials," contains chapters that review immunoisolation of cells by semipermeable membranes and microcapsulation as approaches to xenogeneic cells transplant. These techniques isolate the transplanted cells and prevent access of antibodies and complement proteins to the cells while allowing access to oxygen and nutrients, providing the cells the necessary ingredients to secrete desirable products such as hormones. In addition, part II discusses strategies for creating biomimetic materials designed to control cell behavior and direct organspecific tissue regeneration. Part III, "Encapsulation: Fundamentals and Applications," details various types of encapsulation and the use of this technology for somatic gene therapy. The remaining four parts deal with the use of this technology in specific organ transplantation. As its title indicates, part IV, "Islet Isolation, Transplantation and the Bioartificial Pancreas," discusses the recent scientific progress made in islet cell isolation, preservation, transplantation, and the application of microencapsulation in treating type I diabetes. Part V, "Biological Aspects," discusses the immunologic processes that impede the use of islet transplantation of encapsulated genetically engineered cells for long-term, constant delivery of peptides in diabetics. Part VI, "Bioartificial Liver," discusses the design and evaluation of a bioartificial liver that would perform the functions of oxidative detoxification, metabolism, excretion, and modulation of immune and hormonal systems. Part VII, "Skin, Bone, Cartilage and Craniofacial Issues," discusses tissue engineering for craniofacial bone, computer-controlled bioreactor for large-scale production of cultured skin grafts, and tissue engineering of cartilage by cell seeding on bioabsorbable scaffolds. As expected from the Annals of the New York Academy of Sciences, this volume presents the latest scientific achievements in the subject discussed. Although the subject of bioartificial organs is not one that a vascular surgeon deals with clinically, the concepts discussed are highly interesting and indicate what is coming down the pike in the management of diabetes, the complications of which vascular surgeons deal with every day. The subjects discussed are important, technology driven, and quite novel. I enjoyed reading this book and found it very interesting and stimulating.

Research paper thumbnail of Towards the First Principles in Biology and Cancer: New Vistas in Computational Systems Biology of Cancer

Life

These days many leading scientists argue for a new paradigm for cancer research and propose a com... more These days many leading scientists argue for a new paradigm for cancer research and propose a complex systems-view of cancer supported by empirical evidence. As an example, Thea Newman (2021) has applied “the lessons learned from physical systems to a critique of reductionism in medical research, with an emphasis on cancer”. It is the understanding of this author that the mesoscale constructs that combine the bottom-up as well as top-down approaches, are very close to the concept of emergence. The mesoscale constructs can be said to be those effective components through which the system allows itself to be understood. A short list of basic concepts related to life/biology fundamentals are first introduced to demonstrate a lack of emphasis on these matters in literature. It is imperative that physical and chemical approaches are introduced and incorporated in biology to make it more conceptually sound, quantitative, and based on the first principles. Non-equilibrium thermodynamics is...

Research paper thumbnail of Systems Biology: Integrative Biology and Simulation Tools

Growth in the pharmaceutical market has slowed down almost to a standstill. One reason is that go... more Growth in the pharmaceutical market has slowed down almost to a standstill. One reason is that governments and other payers are cutting costs in a faltering world economy. But a more fundamental problem is the failure of major companies to discover, develop and market new drugs. Major drugs losing patent protection or being withdrawn from the market are simply not being replaced by new therapies the pharmaceutical market model is no longer functioning effectively and most pharmaceutical companies are failing to produce the innovation needed for success. This multi-authored new book looks at a vital strategy which can bring innovation to a market in need of new ideas and new products: Systems Biology (SB). Modeling is a significant task of systems biology. SB aims to develop and use efficient algorithms, data structures, visualization and communication tools to orchestrate the integration of large quantities of biological data with the goal of computer modeling. It involves the use o...

Research paper thumbnail of Air-water interfacial protein model for egg albumin and cellulase

Research paper thumbnail of Water Soluble Polymers for Immunoisolation II: Evaluation of Multicomponent Microencapsulation Systems

Advances in Polymer Science

Microcapsules have been prepared via a complex coacervation reaction from polyanion and polycatio... more Microcapsules have been prepared via a complex coacervation reaction from polyanion and polycation mixtures. Multicomponent blends of synthetic, semi-synthetic and naturally oc curring macromolecules have been evaluated with a particular interest in the preparation of immunoisolation barriers for pancreatic islets. A screening has resulted in thirty three poly meric systems which have been compared according to their mechanical strength, capsule characteristics (such as shape, surface smoothness, stability, and swelling/shrinking) and per meability (MWCO). A limited number of tests were also carried out on the cell viability in the presence of polymer mixtures. These included measurements of the perifusion of encapsulat ed pancreatic islets and the host tissue response. The quality of the membrane produced was observed to be a strong function of the polymer properties, processing conditions, such as the type and concentration of the simple electrolyte, and the reaction time. Additionally, a multicomponent polyelectrolyte technology based on the formation of a capsular "wall-complex" was developed for the simultaneous optimization of the membrane mechanical properties and permeability. This involved the preparation of a wide pore matrix through the reaction of a core polyanion with a small ionotropic ion. A second, low or medium molecular weight, polycation was subsequently added, in a second reactive stage, and its time dependent diffusion into the capsule could be used to control the membrane wall thickness and permeability. Alternative ly, the simultaneous application of low and high molecular weight cations (or a divalent and polyvalent cation) often led to capsules with similar controllable properties. For many combi nations, a distinct capsular wall was observed. Overall seven chemistries were identified as pro viding suitable permeability and capsular mechanical properties. All contained an anionic polysaccharide blend interior to an oligocation solution. The alginate/cellulose sulfate//polymethylene-co-guanidine/calcium chloride/sodium chloride system was found to be the most viable alternative to the standard alginate-polylysine-alginate capsule (APA).

Research paper thumbnail of Parameter effecting the experimental determination of modal properties

Vibroengineering PROCEDIA

The investigation of structure dynamic behavior can be done by finding modal properties, which ar... more The investigation of structure dynamic behavior can be done by finding modal properties, which are based on the relation of force and vibration response. The information about exact force value is in most application unknown, but the investigation can be done for unit magnitude of force. In case of using modal exciter, the force applied to structure is transferred by connection between modal exciter and structure. The stiffness of connection part can affect the dynamic behavior and change the modal properties, or response of investigated part, because of additional modal properties of connection part. This article is focused on the influence of two different rod stiffness during modal properties identification. The modal exciter is used to find potential critical frequency during decreasing frequency. The affect is evaluated on the surface normal velocity as well as acoustic pressure. The stiffness of rod significantly affects the dynamic response of structure and based on that can add frequency, when the emitted noise is high.

Research paper thumbnail of Overview of Present Problems Facing Commercialization of Nanomedicines

Intracellular Delivery III, 2016

Research paper thumbnail of Effect of �-cyclodextrin in artificial chaperones assisted foam fractionation of cellulase

Appl Biochem Biotech, 2006

Research paper thumbnail of Degeneration of �-glucosidase activity in a foam fractionation process

Appl Biochem Biotech, 2004

Research paper thumbnail of Nanoparticulate composition for efficient gene transfer

Research paper thumbnail of Air-water interfacial protein model for egg albumin and cellulase

Rbcf Revista Brasileira De Ciencias Farmaceuticas, 2001

Research paper thumbnail of Encapsulation system for the immunoisolation of living cells

Research paper thumbnail of The effect of pH on the foam fractionation of �-glucosidase and cellulase

Bioresource Technol, 2003

Research paper thumbnail of Systems Analysis and Synthesis in Biology and Biotechnology : International Journal of General Systems

Research paper thumbnail of Drug delivery system exhibiting permeability control

Research paper thumbnail of Polymeric encapsulation system promoting angiogenesis

Research paper thumbnail of System and methods for measuring at least one metabolic rate of a plurality of cells

Research paper thumbnail of NanoLiterBioReactor: Long-Term Mammalian Cell Culture at Nanofabricated Scale

Biomedical Microdevices, 2004

Research paper thumbnail of System and Methods for Discriminating an Agent

Research paper thumbnail of Capillary perfused bioreactors with multiple chambers

A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present in... more A bioreactor for cultivating living cells in a liquid medium. In one embodiment of the present invention, the bioreactor includes a first substrate having a first surface, an opposite second surface and edges. The bioreactor further includes a second substrate having a first surface and an opposite second surface, defining a cavity with a bottom surface, where the bottom surface is located therebetween the first surface and the second surface. The first surface of the first substrate is received by the second surface of the second substrate to cover the ...

Research paper thumbnail of Bioartificial organs II: technology, medicine & materials

Journal of Vascular Surgery, 2000

together 94 participants that represented medicine, biology, material science, and engineering to... more together 94 participants that represented medicine, biology, material science, and engineering to spend 5 days debating the subject. This book, based on the proceedings of this meeting, represents volume 875 of the Annals of the New York Academy of Sciences. It is edited by the conference organizers. The book is more than 400 pages and organized in seven parts, containing a total of 35 chapters. The first part is written by the main editor, and it provides an overview of the risks and requirements of bioartificial organs. Part II, "Novel Materials," contains chapters that review immunoisolation of cells by semipermeable membranes and microcapsulation as approaches to xenogeneic cells transplant. These techniques isolate the transplanted cells and prevent access of antibodies and complement proteins to the cells while allowing access to oxygen and nutrients, providing the cells the necessary ingredients to secrete desirable products such as hormones. In addition, part II discusses strategies for creating biomimetic materials designed to control cell behavior and direct organspecific tissue regeneration. Part III, "Encapsulation: Fundamentals and Applications," details various types of encapsulation and the use of this technology for somatic gene therapy. The remaining four parts deal with the use of this technology in specific organ transplantation. As its title indicates, part IV, "Islet Isolation, Transplantation and the Bioartificial Pancreas," discusses the recent scientific progress made in islet cell isolation, preservation, transplantation, and the application of microencapsulation in treating type I diabetes. Part V, "Biological Aspects," discusses the immunologic processes that impede the use of islet transplantation of encapsulated genetically engineered cells for long-term, constant delivery of peptides in diabetics. Part VI, "Bioartificial Liver," discusses the design and evaluation of a bioartificial liver that would perform the functions of oxidative detoxification, metabolism, excretion, and modulation of immune and hormonal systems. Part VII, "Skin, Bone, Cartilage and Craniofacial Issues," discusses tissue engineering for craniofacial bone, computer-controlled bioreactor for large-scale production of cultured skin grafts, and tissue engineering of cartilage by cell seeding on bioabsorbable scaffolds. As expected from the Annals of the New York Academy of Sciences, this volume presents the latest scientific achievements in the subject discussed. Although the subject of bioartificial organs is not one that a vascular surgeon deals with clinically, the concepts discussed are highly interesting and indicate what is coming down the pike in the management of diabetes, the complications of which vascular surgeons deal with every day. The subjects discussed are important, technology driven, and quite novel. I enjoyed reading this book and found it very interesting and stimulating.

Research paper thumbnail of Towards the First Principles in Biology and Cancer: New Vistas in Computational Systems Biology of Cancer

Life

These days many leading scientists argue for a new paradigm for cancer research and propose a com... more These days many leading scientists argue for a new paradigm for cancer research and propose a complex systems-view of cancer supported by empirical evidence. As an example, Thea Newman (2021) has applied “the lessons learned from physical systems to a critique of reductionism in medical research, with an emphasis on cancer”. It is the understanding of this author that the mesoscale constructs that combine the bottom-up as well as top-down approaches, are very close to the concept of emergence. The mesoscale constructs can be said to be those effective components through which the system allows itself to be understood. A short list of basic concepts related to life/biology fundamentals are first introduced to demonstrate a lack of emphasis on these matters in literature. It is imperative that physical and chemical approaches are introduced and incorporated in biology to make it more conceptually sound, quantitative, and based on the first principles. Non-equilibrium thermodynamics is...

Research paper thumbnail of Systems Biology: Integrative Biology and Simulation Tools

Growth in the pharmaceutical market has slowed down almost to a standstill. One reason is that go... more Growth in the pharmaceutical market has slowed down almost to a standstill. One reason is that governments and other payers are cutting costs in a faltering world economy. But a more fundamental problem is the failure of major companies to discover, develop and market new drugs. Major drugs losing patent protection or being withdrawn from the market are simply not being replaced by new therapies the pharmaceutical market model is no longer functioning effectively and most pharmaceutical companies are failing to produce the innovation needed for success. This multi-authored new book looks at a vital strategy which can bring innovation to a market in need of new ideas and new products: Systems Biology (SB). Modeling is a significant task of systems biology. SB aims to develop and use efficient algorithms, data structures, visualization and communication tools to orchestrate the integration of large quantities of biological data with the goal of computer modeling. It involves the use o...

Research paper thumbnail of Air-water interfacial protein model for egg albumin and cellulase

Research paper thumbnail of Water Soluble Polymers for Immunoisolation II: Evaluation of Multicomponent Microencapsulation Systems

Advances in Polymer Science

Microcapsules have been prepared via a complex coacervation reaction from polyanion and polycatio... more Microcapsules have been prepared via a complex coacervation reaction from polyanion and polycation mixtures. Multicomponent blends of synthetic, semi-synthetic and naturally oc curring macromolecules have been evaluated with a particular interest in the preparation of immunoisolation barriers for pancreatic islets. A screening has resulted in thirty three poly meric systems which have been compared according to their mechanical strength, capsule characteristics (such as shape, surface smoothness, stability, and swelling/shrinking) and per meability (MWCO). A limited number of tests were also carried out on the cell viability in the presence of polymer mixtures. These included measurements of the perifusion of encapsulat ed pancreatic islets and the host tissue response. The quality of the membrane produced was observed to be a strong function of the polymer properties, processing conditions, such as the type and concentration of the simple electrolyte, and the reaction time. Additionally, a multicomponent polyelectrolyte technology based on the formation of a capsular "wall-complex" was developed for the simultaneous optimization of the membrane mechanical properties and permeability. This involved the preparation of a wide pore matrix through the reaction of a core polyanion with a small ionotropic ion. A second, low or medium molecular weight, polycation was subsequently added, in a second reactive stage, and its time dependent diffusion into the capsule could be used to control the membrane wall thickness and permeability. Alternative ly, the simultaneous application of low and high molecular weight cations (or a divalent and polyvalent cation) often led to capsules with similar controllable properties. For many combi nations, a distinct capsular wall was observed. Overall seven chemistries were identified as pro viding suitable permeability and capsular mechanical properties. All contained an anionic polysaccharide blend interior to an oligocation solution. The alginate/cellulose sulfate//polymethylene-co-guanidine/calcium chloride/sodium chloride system was found to be the most viable alternative to the standard alginate-polylysine-alginate capsule (APA).

Research paper thumbnail of Parameter effecting the experimental determination of modal properties

Vibroengineering PROCEDIA

The investigation of structure dynamic behavior can be done by finding modal properties, which ar... more The investigation of structure dynamic behavior can be done by finding modal properties, which are based on the relation of force and vibration response. The information about exact force value is in most application unknown, but the investigation can be done for unit magnitude of force. In case of using modal exciter, the force applied to structure is transferred by connection between modal exciter and structure. The stiffness of connection part can affect the dynamic behavior and change the modal properties, or response of investigated part, because of additional modal properties of connection part. This article is focused on the influence of two different rod stiffness during modal properties identification. The modal exciter is used to find potential critical frequency during decreasing frequency. The affect is evaluated on the surface normal velocity as well as acoustic pressure. The stiffness of rod significantly affects the dynamic response of structure and based on that can add frequency, when the emitted noise is high.

Research paper thumbnail of Overview of Present Problems Facing Commercialization of Nanomedicines

Intracellular Delivery III, 2016

Research paper thumbnail of Effect of �-cyclodextrin in artificial chaperones assisted foam fractionation of cellulase

Appl Biochem Biotech, 2006

Research paper thumbnail of Degeneration of �-glucosidase activity in a foam fractionation process

Appl Biochem Biotech, 2004

Research paper thumbnail of Nanoparticulate composition for efficient gene transfer

Research paper thumbnail of Air-water interfacial protein model for egg albumin and cellulase

Rbcf Revista Brasileira De Ciencias Farmaceuticas, 2001

Research paper thumbnail of Encapsulation system for the immunoisolation of living cells

Research paper thumbnail of The effect of pH on the foam fractionation of �-glucosidase and cellulase

Bioresource Technol, 2003

Research paper thumbnail of Systems Analysis and Synthesis in Biology and Biotechnology : International Journal of General Systems

Research paper thumbnail of Drug delivery system exhibiting permeability control

Research paper thumbnail of Polymeric encapsulation system promoting angiogenesis

Research paper thumbnail of System and methods for measuring at least one metabolic rate of a plurality of cells