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Papers by purnima singh

Acta Botanica Malacitana, 2020

ABSTRACT. Biodiversity and distribution of cyanobacteria at Dronning Maud Land, East Antarctica.T... more ABSTRACT. Biodiversity and distribution of cyanobacteria at Dronning Maud Land, East Antarctica.The current study describes the biodiversity and distribution of cyanobacteria from the natural habitats of Schirmacher land, East Antarctica surveyed during 23rd Indian Antarctic Expedition (2003–2004). Cyanobacteria were mapped using the Global Positioning System (GPS). A total of 109 species (91 species were non-heterocystous and 18 species were heterocystous) from 30 genera and 9 families were recorded; 67, 86 and 14 species of cyanobacteria were identified at altitudes of sea level >100 m, 101–150 m and 398–461 m, respectively. The relative frequency and relative density of cyanobacterial populations in the microbial mats showed that 11 species from 8 genera were abundant and 6 species (Phormidium angustissimum, P. tenue, P. uncinatum Schizothrix vaginata, Nostoc kihlmanii and Plectonema terebrans) could be considered as dominant species in the study area.Key words. Antarctic, cya...

Genome announcements, Jan 13, 2017

Nesterenkonia sp. strain PF2B19, a psychrophilic bacterium, was isolated from 44,800-year-old per... more Nesterenkonia sp. strain PF2B19, a psychrophilic bacterium, was isolated from 44,800-year-old permafrost. The draft genome sequence of this strain revealed the presence of genes involved in the production of cold active enzymes, carotenoid biosynthesis, fatty acid biosynthesis, and resistance to heavy metals. These results show the immense potential of the strain.

Genome announcements, Jan 13, 2017

Marinobacter sp. strain AC-23 was isolated from Kongsfjorden in the Arctic. Here, we report the f... more Marinobacter sp. strain AC-23 was isolated from Kongsfjorden in the Arctic. Here, we report the first draft genome sequence of a putative novel species of the genus Marinobacter comprising 4,149,715 bp, with a mean G+C content of 54.4%. The draft genome sequence will aid in understanding the psychrophilic and sea ice-specific lifestyle.

Polar Research, 2011

Many filamentous fungi were isolated from the soils of Ny-Å lesund, Spitsbergen, Svalbard, and we... more Many filamentous fungi were isolated from the soils of Ny-Å lesund, Spitsbergen, Svalbard, and were screened in vitro for their phosphate solubilizing ability. Two strains of Aspergillus niger showed good tricalcium phosphate (TCP) solubilizing ability in Pikovskaya's medium. The TCP solubilization index was calculated at varying levels of pH and temperatures. The ability of Aspergillus niger strain-1 to solubilize and release inorganic-P was 285 mg ml Á1 , while Aspergillus niger strain-2 solubilized 262 mg ml Á1 from 0.5% TCP after seven days. This is the first report of TCP solubilization by Arctic strains that may serve as very good phosphate solubilizers in the form of biofertilizer.

Polar Biology, 2012

Environmental stress in the Arctic region leads to damage in plant membranes as a result of oxida... more Environmental stress in the Arctic region leads to damage in plant membranes as a result of oxidation processes. To withstand these stress conditions, plants are expected to produce antioxidants that differ from phenolics. Here we investigated the chemical composition and antioxidative activities of four Arctic flowering plant species (Dryas octopetala, Carex rupestris, Silene uralensis, and Deschampsia alpina.) through in vitro measurements of the Free Radical Scavenging activities (FRS), Inhibition of Lipid Peroxidation (ILP) and Trolox Equivalent Antioxidant Capacities (TEAC). D. octopetala exhibited the highest ILP (76.45%) and FRS (86.58%) activities. The TEAC values were higher than those of the Trolox vitamin E standard in all four species. Overall, the antioxidative activity was highest in D. octopetala, followed by C. rupestris, S. uralensis, and D. alpina. Electrospray ionization tandem mass spectrometric (ESI-MS/MS) analysis of methanolic extracts of these plants revealed the presence of organoselenides, linear alkylbenzenesulfonates (LAS) and oligosaccharides, some of which are reported as antioxidants in the literature. Hence, it is likely that the antioxidant activities exhibited by these plants are not only related to the production of phenolics. This is the first report of the antioxidant potential of four Arctic flowering plants and the presence of selenides in D. octopetala and S. uralensis, and the production of LAS in C. rupestris. Our findings suggest that these plants can be used as nutraceutical sources of selenium and as biomarkers for environmental pollution.

Fungi in Polar Regions, 2019

Федеральное государственное бюджетное образовательное учреждение высшего образования «Курский гос... more Федеральное государственное бюджетное образовательное учреждение высшего образования «Курский государственный медицинский университет» Министерства здравоохранения Российской Федерации: 305041, Курск, ул. К.Маркса, 3; 2 -Бюджетное медицинское учреждение «Курская областная клиническая больница» Комитета здравоохранения Курской области: 305007, Курск, ул. Сумская, 45а Информация об авторах Прибылов Сергей Александрович -д. м. н., профессор, заведующий кафедрой внутренних болезней факультета последипломного образования Федерального государственного бюджетного образовательного учреждения высшего образования «Курский государственный медицинский универси тет» Министерства здравоохранения Российской Федерации, заместитель главного врача по медицинской части Бюджетного медицинского учрежде ния «Курская областная клиническая больница» Комитета здравоохранения Курской области; тел.:

Marine Genomics, 2015

Obligate psychrophilic, Cryobacterium sp. MLB-32, was isolated from cryoconite holes of high Arct... more Obligate psychrophilic, Cryobacterium sp. MLB-32, was isolated from cryoconite holes of high Arctic glaciers. Here, we report the first draft genome sequence of the putative novel species of the genus Cryobacterium, providing opportunities for biotechnological and agricultural exploitation of its genome features.

FEMS Microbiology Letters, 2013

Fourteen Arctic bacterial strains belonging to five genera, Cryobacterium, Leifsonia, Polaromonas... more Fourteen Arctic bacterial strains belonging to five genera, Cryobacterium, Leifsonia, Polaromonas, Pseudomonas, and Subtercola isolated from sediments found in cryoconite holes of Arctic glaciers, were subjected to screening for antifreeze proteins (AFPs). Eight strains showed AFP activity, and six strains of four species were further characterized. Pseudomonas ficuserectae exhibited a high thermal hysteresis (TH) activity. Ice recrystallization inhibition (IRI) activity was observed in most cultures at low protein concentration. Bacterial AFPs produced rounded shape of ice crystals that did not change their size and morphology within the TH window. Cry-g (P. ficuserectae) failed to inhibit ice recrystallization, indicating that the IRI activity of the AFPs does not relate to the strength of TH activity. SDS-PAGE analysis of the AFPs suggests their apparent molecular weights to be around 23 kDa. This study is significant as it screens several species of Arctic bacterial strains for AFP activity. So far, only one species of bacteria, Pseudomonas putida, was reported from the Arctic to produce AFPs. N-terminal amino acid sequence analysis shows that the bacterial AFPs isolated belong to the AFP family IBP-1, which is known to have an important physiological role in the cold environment. AFPs of glacier cryoconite habitat have been discussed.

Diversity, cold active enzymes and adaptation strategies of bacteria inhabiting glacier cryoconite holes of High Arctic

Extremophiles, 2013

Cryoconite holes have biogeochemical, ecological and biotechnological importance. This communicat... more Cryoconite holes have biogeochemical, ecological and biotechnological importance. This communication presents results on culturable psychrophilic bacterial diversity from cryoconite holes at Midre Lovénbreen (ML), Austre Brøggerbreen (AB), and Vestre Brøggerbreen (VB) glaciers. The culturable bacterial count ranged from 2.7 × 10(3) to 8.8 × 10(4) CFUs/g while the total bacterial numbers ranged from 5.07 × 10(5) to 1.50 × 10(6) cells at the three glaciers. A total of 35 morphologically distinct bacterial isolates were isolated. Based on 16S rRNA gene sequence data, the identified species belonged to eight genera namely Pseudomonas, Polaromonas, Micrococcus, Subtercola, Agreia, Leifsonia, Cryobacterium and Flavobacterium. The isolates varied in their growth temperature, NaCl tolerance, growth pH, enzyme activities, carbon utilization and antibiotic sensitivity tests. Fatty acid profiles indicate the predominance of branched fatty acids in the isolates. To the best of our knowledge, this is the first record of culturable bacterial communities and their characterization from glacier cryoconites from High Arctic. High amylase and protease activities expressed by Micrococcus sp. MLB-41 and amylase, protease and lipase activities expressed by Cryobacterium sp. MLB-32 provide a clue to the potential applications of these organisms. These cold-adapted enzymes may provide an opportunity for the prospect of biotechnology in Arctic.

Cryobiology, 2014

A psychrophilic yeast species was isolated from glacier cryoconite holes of Svalbard. Nucleotide ... more A psychrophilic yeast species was isolated from glacier cryoconite holes of Svalbard. Nucleotide sequences of the strains were studied using D1/D2 domain, ITS region and partial sequences of mitochondrial cytochrome b gene. The strains belonged to a clade of psychrophilic yeasts, but showed marked differences from related species in the D1/D2 domain and biochemical characters. Effects of temperature, salt and media on growth of the cultures were also studied. Screening of the cultures for amylase, cellulase, protease, lipase, urease and catalase activities was carried out. The strains expressed high amylase and lipase activities. Freeze tolerance ability of the isolates indicated the formation of unique hexagonal ice crystal structures due to presence of 'antifreeze proteins' (AFPs). FAME analysis of cultures showed a unique trend of increase in unsaturated fatty acids with decrease in temperature. The major fatty acids recorded were oleic acid, linoleic acid, linolenic acid, palmitic acid, stearic acid, myristic acid and pentadecanoic acid. Based on sequence data and, physiological and morphological properties of the strains, we propose a novel species, Rhodotorula svalbardensis and designate strains MLB-I (CCP-II) and CRY-YB-1 (CBS 12863, JCM 19699, JCM 19700, MTCC 10952) as its type strains (Etymology: sval.bar.den 0 sis. N.L. fem. adj. svalbardensis pertaining to Svalbard).

Cryobiology, 2013

Ten strains of cryophilic yeast were studied from glacier ice cores of Svalbard, Arctic. The ice ... more Ten strains of cryophilic yeast were studied from glacier ice cores of Svalbard, Arctic. The ice melt samples contained about 3 Â 10 3 -1 Â 10 4 colony forming unit (CFUs) per ml. Sequence analysis of the isolates, using D1/D2 domain identified five species of yeasts: Cryptococcus adeliensis (MLB-18 JX192655), Cryptococcus albidosimilis (MLB-19 JX192656), Cryptococcus saitoi (MLB-22 JX192659), Rhodosporidium lusitaniae (MLB-20 JX192657), and Rhodotorula mucilaginosa (MLB-27 JX192664). Effect of temperature on growth of these isolates was studied. The strains are able to grow at temperatures ranging between 1 and 20 °C. Screening of the cultures for amylase, cellulase, protease, lipase, urease and catalase activity were carried out indicating varying amounts of enzyme production at different temperatures. Characterization of lipase in strain Cryptococcus sp. MLB-24 was performed. Fatty acid methyl ester (FAME) analysis of the cultures grown at four different temperatures (1, 4, 15, and 20 °C) was also done. Decrease in temperature was reported to cause increase in concentration of unsaturated fatty acids. High amount of oleic acid accumulated with increase in temperature. These fatty acids possibly help the strains to survive in glacial ice core cold environment. The extracellular and intracellular filtrate of the cultures showed negative antifreeze protein (AFP) activity. The observations indicate that probably the isolates in the present undertaking adapt to low temperatures, by enzyme and PUFA secretion rather than by antifreeze protein secretion.

Polar Biology, 2011

Antioxidants are compounds that scavenge the free radicals produced in living organisms. The anti... more Antioxidants are compounds that scavenge the free radicals produced in living organisms. The antioxidant potential of eight Arctic lichen species was evaluated in vitro using free radical scavenging activity (FRS), inhibition of lipid peroxidation (ILP), and Trolox equivalent antioxidant capacity assay (TEAC). FRS activities of lichen species in various organic solvents such as methanol, ethanol, acetone, and dimethyl sulphoxide (DMSO) were in the range 9.6-51.77%, while ILP activities in these solvents ranged from 32.5 to 82.43%. Pseudophebe pubescens showed the highest ILP (82.43%) and FRS (51.77%) activities as compared to other lichen species and the standard antioxidants butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). The TEAC value was also found to be higher in all species compared to the standard water soluble vitamin E analog Trolox (3.9 mM). The order of antioxidative activities in lichen species was Pseudophebe pubescens [ Cladonia amaurocraea [ Cladonia mediterranea [ Physcia caesia [ Flavocetraria nivalis [ Cetraria fastigata [ Xanthoria elegans [ Umbilicaria hyperborea. This is the first report of the measurement of antioxidant potential in Arctic lichens.

Acta Botanica Malacitana, 2020

ABSTRACT. Biodiversity and distribution of cyanobacteria at Dronning Maud Land, East Antarctica.T... more ABSTRACT. Biodiversity and distribution of cyanobacteria at Dronning Maud Land, East Antarctica.The current study describes the biodiversity and distribution of cyanobacteria from the natural habitats of Schirmacher land, East Antarctica surveyed during 23rd Indian Antarctic Expedition (2003–2004). Cyanobacteria were mapped using the Global Positioning System (GPS). A total of 109 species (91 species were non-heterocystous and 18 species were heterocystous) from 30 genera and 9 families were recorded; 67, 86 and 14 species of cyanobacteria were identified at altitudes of sea level >100 m, 101–150 m and 398–461 m, respectively. The relative frequency and relative density of cyanobacterial populations in the microbial mats showed that 11 species from 8 genera were abundant and 6 species (Phormidium angustissimum, P. tenue, P. uncinatum Schizothrix vaginata, Nostoc kihlmanii and Plectonema terebrans) could be considered as dominant species in the study area.Key words. Antarctic, cya...

Genome announcements, Jan 13, 2017

Nesterenkonia sp. strain PF2B19, a psychrophilic bacterium, was isolated from 44,800-year-old per... more Nesterenkonia sp. strain PF2B19, a psychrophilic bacterium, was isolated from 44,800-year-old permafrost. The draft genome sequence of this strain revealed the presence of genes involved in the production of cold active enzymes, carotenoid biosynthesis, fatty acid biosynthesis, and resistance to heavy metals. These results show the immense potential of the strain.

Genome announcements, Jan 13, 2017

Marinobacter sp. strain AC-23 was isolated from Kongsfjorden in the Arctic. Here, we report the f... more Marinobacter sp. strain AC-23 was isolated from Kongsfjorden in the Arctic. Here, we report the first draft genome sequence of a putative novel species of the genus Marinobacter comprising 4,149,715 bp, with a mean G+C content of 54.4%. The draft genome sequence will aid in understanding the psychrophilic and sea ice-specific lifestyle.

Polar Research, 2011

Many filamentous fungi were isolated from the soils of Ny-Å lesund, Spitsbergen, Svalbard, and we... more Many filamentous fungi were isolated from the soils of Ny-Å lesund, Spitsbergen, Svalbard, and were screened in vitro for their phosphate solubilizing ability. Two strains of Aspergillus niger showed good tricalcium phosphate (TCP) solubilizing ability in Pikovskaya's medium. The TCP solubilization index was calculated at varying levels of pH and temperatures. The ability of Aspergillus niger strain-1 to solubilize and release inorganic-P was 285 mg ml Á1 , while Aspergillus niger strain-2 solubilized 262 mg ml Á1 from 0.5% TCP after seven days. This is the first report of TCP solubilization by Arctic strains that may serve as very good phosphate solubilizers in the form of biofertilizer.

Polar Biology, 2012

Environmental stress in the Arctic region leads to damage in plant membranes as a result of oxida... more Environmental stress in the Arctic region leads to damage in plant membranes as a result of oxidation processes. To withstand these stress conditions, plants are expected to produce antioxidants that differ from phenolics. Here we investigated the chemical composition and antioxidative activities of four Arctic flowering plant species (Dryas octopetala, Carex rupestris, Silene uralensis, and Deschampsia alpina.) through in vitro measurements of the Free Radical Scavenging activities (FRS), Inhibition of Lipid Peroxidation (ILP) and Trolox Equivalent Antioxidant Capacities (TEAC). D. octopetala exhibited the highest ILP (76.45%) and FRS (86.58%) activities. The TEAC values were higher than those of the Trolox vitamin E standard in all four species. Overall, the antioxidative activity was highest in D. octopetala, followed by C. rupestris, S. uralensis, and D. alpina. Electrospray ionization tandem mass spectrometric (ESI-MS/MS) analysis of methanolic extracts of these plants revealed the presence of organoselenides, linear alkylbenzenesulfonates (LAS) and oligosaccharides, some of which are reported as antioxidants in the literature. Hence, it is likely that the antioxidant activities exhibited by these plants are not only related to the production of phenolics. This is the first report of the antioxidant potential of four Arctic flowering plants and the presence of selenides in D. octopetala and S. uralensis, and the production of LAS in C. rupestris. Our findings suggest that these plants can be used as nutraceutical sources of selenium and as biomarkers for environmental pollution.

Fungi in Polar Regions, 2019

Федеральное государственное бюджетное образовательное учреждение высшего образования «Курский гос... more Федеральное государственное бюджетное образовательное учреждение высшего образования «Курский государственный медицинский университет» Министерства здравоохранения Российской Федерации: 305041, Курск, ул. К.Маркса, 3; 2 -Бюджетное медицинское учреждение «Курская областная клиническая больница» Комитета здравоохранения Курской области: 305007, Курск, ул. Сумская, 45а Информация об авторах Прибылов Сергей Александрович -д. м. н., профессор, заведующий кафедрой внутренних болезней факультета последипломного образования Федерального государственного бюджетного образовательного учреждения высшего образования «Курский государственный медицинский универси тет» Министерства здравоохранения Российской Федерации, заместитель главного врача по медицинской части Бюджетного медицинского учрежде ния «Курская областная клиническая больница» Комитета здравоохранения Курской области; тел.:

Marine Genomics, 2015

Obligate psychrophilic, Cryobacterium sp. MLB-32, was isolated from cryoconite holes of high Arct... more Obligate psychrophilic, Cryobacterium sp. MLB-32, was isolated from cryoconite holes of high Arctic glaciers. Here, we report the first draft genome sequence of the putative novel species of the genus Cryobacterium, providing opportunities for biotechnological and agricultural exploitation of its genome features.

FEMS Microbiology Letters, 2013

Fourteen Arctic bacterial strains belonging to five genera, Cryobacterium, Leifsonia, Polaromonas... more Fourteen Arctic bacterial strains belonging to five genera, Cryobacterium, Leifsonia, Polaromonas, Pseudomonas, and Subtercola isolated from sediments found in cryoconite holes of Arctic glaciers, were subjected to screening for antifreeze proteins (AFPs). Eight strains showed AFP activity, and six strains of four species were further characterized. Pseudomonas ficuserectae exhibited a high thermal hysteresis (TH) activity. Ice recrystallization inhibition (IRI) activity was observed in most cultures at low protein concentration. Bacterial AFPs produced rounded shape of ice crystals that did not change their size and morphology within the TH window. Cry-g (P. ficuserectae) failed to inhibit ice recrystallization, indicating that the IRI activity of the AFPs does not relate to the strength of TH activity. SDS-PAGE analysis of the AFPs suggests their apparent molecular weights to be around 23 kDa. This study is significant as it screens several species of Arctic bacterial strains for AFP activity. So far, only one species of bacteria, Pseudomonas putida, was reported from the Arctic to produce AFPs. N-terminal amino acid sequence analysis shows that the bacterial AFPs isolated belong to the AFP family IBP-1, which is known to have an important physiological role in the cold environment. AFPs of glacier cryoconite habitat have been discussed.

Diversity, cold active enzymes and adaptation strategies of bacteria inhabiting glacier cryoconite holes of High Arctic

Extremophiles, 2013

Cryoconite holes have biogeochemical, ecological and biotechnological importance. This communicat... more Cryoconite holes have biogeochemical, ecological and biotechnological importance. This communication presents results on culturable psychrophilic bacterial diversity from cryoconite holes at Midre Lovénbreen (ML), Austre Brøggerbreen (AB), and Vestre Brøggerbreen (VB) glaciers. The culturable bacterial count ranged from 2.7 × 10(3) to 8.8 × 10(4) CFUs/g while the total bacterial numbers ranged from 5.07 × 10(5) to 1.50 × 10(6) cells at the three glaciers. A total of 35 morphologically distinct bacterial isolates were isolated. Based on 16S rRNA gene sequence data, the identified species belonged to eight genera namely Pseudomonas, Polaromonas, Micrococcus, Subtercola, Agreia, Leifsonia, Cryobacterium and Flavobacterium. The isolates varied in their growth temperature, NaCl tolerance, growth pH, enzyme activities, carbon utilization and antibiotic sensitivity tests. Fatty acid profiles indicate the predominance of branched fatty acids in the isolates. To the best of our knowledge, this is the first record of culturable bacterial communities and their characterization from glacier cryoconites from High Arctic. High amylase and protease activities expressed by Micrococcus sp. MLB-41 and amylase, protease and lipase activities expressed by Cryobacterium sp. MLB-32 provide a clue to the potential applications of these organisms. These cold-adapted enzymes may provide an opportunity for the prospect of biotechnology in Arctic.

Cryobiology, 2014

A psychrophilic yeast species was isolated from glacier cryoconite holes of Svalbard. Nucleotide ... more A psychrophilic yeast species was isolated from glacier cryoconite holes of Svalbard. Nucleotide sequences of the strains were studied using D1/D2 domain, ITS region and partial sequences of mitochondrial cytochrome b gene. The strains belonged to a clade of psychrophilic yeasts, but showed marked differences from related species in the D1/D2 domain and biochemical characters. Effects of temperature, salt and media on growth of the cultures were also studied. Screening of the cultures for amylase, cellulase, protease, lipase, urease and catalase activities was carried out. The strains expressed high amylase and lipase activities. Freeze tolerance ability of the isolates indicated the formation of unique hexagonal ice crystal structures due to presence of 'antifreeze proteins' (AFPs). FAME analysis of cultures showed a unique trend of increase in unsaturated fatty acids with decrease in temperature. The major fatty acids recorded were oleic acid, linoleic acid, linolenic acid, palmitic acid, stearic acid, myristic acid and pentadecanoic acid. Based on sequence data and, physiological and morphological properties of the strains, we propose a novel species, Rhodotorula svalbardensis and designate strains MLB-I (CCP-II) and CRY-YB-1 (CBS 12863, JCM 19699, JCM 19700, MTCC 10952) as its type strains (Etymology: sval.bar.den 0 sis. N.L. fem. adj. svalbardensis pertaining to Svalbard).

Cryobiology, 2013

Ten strains of cryophilic yeast were studied from glacier ice cores of Svalbard, Arctic. The ice ... more Ten strains of cryophilic yeast were studied from glacier ice cores of Svalbard, Arctic. The ice melt samples contained about 3 Â 10 3 -1 Â 10 4 colony forming unit (CFUs) per ml. Sequence analysis of the isolates, using D1/D2 domain identified five species of yeasts: Cryptococcus adeliensis (MLB-18 JX192655), Cryptococcus albidosimilis (MLB-19 JX192656), Cryptococcus saitoi (MLB-22 JX192659), Rhodosporidium lusitaniae (MLB-20 JX192657), and Rhodotorula mucilaginosa (MLB-27 JX192664). Effect of temperature on growth of these isolates was studied. The strains are able to grow at temperatures ranging between 1 and 20 °C. Screening of the cultures for amylase, cellulase, protease, lipase, urease and catalase activity were carried out indicating varying amounts of enzyme production at different temperatures. Characterization of lipase in strain Cryptococcus sp. MLB-24 was performed. Fatty acid methyl ester (FAME) analysis of the cultures grown at four different temperatures (1, 4, 15, and 20 °C) was also done. Decrease in temperature was reported to cause increase in concentration of unsaturated fatty acids. High amount of oleic acid accumulated with increase in temperature. These fatty acids possibly help the strains to survive in glacial ice core cold environment. The extracellular and intracellular filtrate of the cultures showed negative antifreeze protein (AFP) activity. The observations indicate that probably the isolates in the present undertaking adapt to low temperatures, by enzyme and PUFA secretion rather than by antifreeze protein secretion.

Polar Biology, 2011

Antioxidants are compounds that scavenge the free radicals produced in living organisms. The anti... more Antioxidants are compounds that scavenge the free radicals produced in living organisms. The antioxidant potential of eight Arctic lichen species was evaluated in vitro using free radical scavenging activity (FRS), inhibition of lipid peroxidation (ILP), and Trolox equivalent antioxidant capacity assay (TEAC). FRS activities of lichen species in various organic solvents such as methanol, ethanol, acetone, and dimethyl sulphoxide (DMSO) were in the range 9.6-51.77%, while ILP activities in these solvents ranged from 32.5 to 82.43%. Pseudophebe pubescens showed the highest ILP (82.43%) and FRS (51.77%) activities as compared to other lichen species and the standard antioxidants butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT). The TEAC value was also found to be higher in all species compared to the standard water soluble vitamin E analog Trolox (3.9 mM). The order of antioxidative activities in lichen species was Pseudophebe pubescens [ Cladonia amaurocraea [ Cladonia mediterranea [ Physcia caesia [ Flavocetraria nivalis [ Cetraria fastigata [ Xanthoria elegans [ Umbilicaria hyperborea. This is the first report of the measurement of antioxidant potential in Arctic lichens.