rafael domingues - Academia.edu (original) (raw)
Papers by rafael domingues
Frontiers in Medicine
Selective serotonin reuptake inhibitors (SSRI) are the most common antidepressants used by pregna... more Selective serotonin reuptake inhibitors (SSRI) are the most common antidepressants used by pregnant women. However, adverse pregnancy outcomes have been described in women taking SSRI during pregnancy—placental lesions, premature birth, poor neonatal adaptation. We aimed to investigate the effects of fluoxetine (Prozac® most commonly used SSRI) treatment during the last month of gestation on pregnancy complications, placental and neonatal health in a non-depressed sheep model. On day 119 ± 1 postbreeding (experimental day 0; E0) of a 151-day expected gestation, Hampshire ewes were randomly assigned to receive fluoxetine (n = 9 ewes, 15 lambs; daily intravenously treatment with 10 mg/kg on E0 and E1 and 5 mg/kg daily thereafter until parturition) or to a control group (n = 10; 14 lambs; vehicle only). Blood samples from ewes were collected throughout the experimental period and postpartum; blood from lambs were collected postpartum. Analysis of variance was used for statistical analy...
Reproduction, 2020
Understanding the impacts of nutrition on reproductive physiology in cattle are fundamental to im... more Understanding the impacts of nutrition on reproductive physiology in cattle are fundamental to improve reproductive efficiency for animals under different nutritional conditions. Starting on Day 0 (day of ovulation) until next ovulation, Holstein heifers (n = 24) were fed: low energy diet (ad libitum grass hay; LED) and high energy diet (ad libitum grass hay + concentrate supplement; HED). Heifers on HED gained more weight (average daily gain: 0.824 ± 0.07 vs 0.598 ± 0.09 kg/day) and had increased insulin concentrations. The dominant follicle of wave 1 in HED had greater growth rate overall from Days 0 to 8 and on Days 6–7 and 8–9 and started atresia later. The dominant follicle of wave 2 in HED had greater growth rate overall from Day 9 to 18 and on Days 14–15 and 15–16. In two-wave patterns, there was no difference in estradiol or progesterone concentrations but concentrations of FSH were lower in HED on Days 15 and 16. Estradiol concentrations increased earlier in two-wave patter...
Journal of Dairy Science, 2022
Our objective was to determine the effect of inducing an accessory corpus luteum (CL) with human ... more Our objective was to determine the effect of inducing an accessory corpus luteum (CL) with human chorionic gonadotropin (hCG; 3,300 IU) on d 7 (hCG7) or 2 accessory CL with hCG on d 7 and 13 (hCG7+13) of the estrous cycle in noninseminated lactating Holstein cows. Cows (n = 86) between 39 and 64 DIM were pretreated with an Ovsynch + CIDR protocol, and only synchronized cows were used (n = 64). The day of the last GnRH of Ovsynch was considered d 0 of the estrous cycle. Follicular and luteal dynamics of cows were evaluated daily during an entire estrous cycle by ovarian ultrasonography. Blood samples were collected daily to measure serum concentration of progesterone (P4). Cows were randomly assigned to CON (n = 22, no treatment), hCG7 (n = 20), or hCG7+13 (n = 22) treatments. Two cows from hCG7+13 failed to ovulate after hCG and were removed from the analyses post-hCG treatment. The first day of luteolysis was considered the day that P4 declined to more than 2 SD of the mean for the 4 consecutive P4 concentrations with the greatest mean in late diestrus for each individual cow. The P4 cut-off for complete luteolysis was <1.0 ng/mL. Mean P4 on d 7 (3.23 ± 0.16 ng/mL) did not differ among treatments. Cows treated with hCG had greater total luteal and original CL volume and serum P4 during diestrus than CON. Cows treated with hCG7+13 had greater serum P4 after d 13 of the cycle than hCG7. Cycles were classified as having atypical cycles if the dominant follicle or future dominant follicle at the time of luteolysis did not ovulate (delayed ovulation; CON, n = 2; hCG7, n = 4; hCG7+13, n = 3), had a short cycle (CON, n = 1), delayed (CON, n = 2) or incomplete luteolysis (CON, n = 1; hCG7, n = 4; hCG7+13, n = 5). The remainder of cycles with normal complete luteolysis followed by ovulation were considered to be typical. Based on blood perfusion, the CON cow with incomplete luteolysis had 2 original CL remaining functional after first onset of luteolysis. The rest of the cows with incomplete luteolysis (9/10) had one or more CL regressing and at least one remaining functional after first onset of luteolysis. No specific pattern for CL side (ipsilateral vs. contralateral to a CL with complete regression) was observed for nonregressed CL. Cows with incomplete luteolysis had a second onset of luteolysis to undergo complete functional luteolysis. The proportion of cows with typical cycle was 73% (16/22) for CON, 60% (12/20) for hCG7, and 55% (11/20) for hCG7+13. Cows with typical cycles treated with hCG (hCG7 and hCG7+13) had a later onset of luteolysis, prolonged time to undergo complete luteolysis, and greater proportion of cows with 3 follicular waves than CON, resulting in a longer interovulatory interval for hCG7 and hCG7+13 than CON. In summary, accessory CL induced by hCG during diestrus not only altered follicular and luteal dynamics but also deferred and prolonged the luteolytic process.
Biology of Reproduction, 2022
In heifers and mares, multiple pulses of prostaglandin F2alpha (PGF) are generally associated wit... more In heifers and mares, multiple pulses of prostaglandin F2alpha (PGF) are generally associated with complete luteal regression. Although PGF pulses occur before and during luteolysis, little is known about the role of minor PGF pulses during preluteolysis on subsequent luteal and endometrial PGF production that may initiate luteolysis. Heifers (n = 7/group) and mares (n = 6/group) were treated with a single minor dose of PGF (3.0 and 0.5 mg, respectively) during mid-luteal phase (12 and 10 days postovulation respectively). After treatment, a transient decrease in progesterone (P4) concentrations occurred in heifers between Hours 0 and 2 but at Hour 4 P4 was not different from pretreatment. In mares, P4 was unaltered between Hours 0 and 4. Concentrations of P4 decreased in both species by Hour 24 and complete luteolysis occurred in mares by Hour 48. Luteal and endometrial gene expression were evaluated 4 h posttreatment. In heifers, luteal mRNA abundance of PGF receptor and PGF dehydr...
Toxics, 2022
Selective serotonin reuptake inhibitors (SSRI) are the most common antidepressant used by pregnan... more Selective serotonin reuptake inhibitors (SSRI) are the most common antidepressant used by pregnant women; however, they have been associated with adverse pregnancy outcomes and perinatal morbidity in pregnant women and animal models. We investigated the effects of two SSRI, fluoxetine and sertraline, on pregnancy and neonatal outcomes in mice. Wild-type mice were treated daily with low and high doses of fluoxetine (2 and 20 mg/kg) and sertraline (10 and 20 mg/kg) from the day of detection of a vaginal plug until the end of lactation (21 days postpartum). Pregnancy rate was decreased only in the high dose of fluoxetine group. Maternal weight gain was reduced in the groups receiving the high dose of each drug. Number of pups born was decreased in the high dose of fluoxetine and low and high doses of sertraline while the number of pups weaned was decreased in all SSRI-treated groups corresponding to increased neonatal mortality in all SSRI-treated groups. In conclusion, there was a dos...
International Journal of Advanced Engineering Research and Science, 2018
Bean (Phaseolus vulgaris L.) is a crop of great economic and social impacts in Brazil. This crop ... more Bean (Phaseolus vulgaris L.) is a crop of great economic and social impacts in Brazil. This crop is extremely appreciated by the Brazilian population and an important source of protein. Usually the small farmers are responsible by the largest production of the bean in Brazil. This work deals with the analysis of the effect of different water regimes (35, 28, 21 and 14%)on the porous system of a soil cropped with two distinct cultivars (Campos Gerais and Tuiuiú). Soil water retention curve (SWRC) and its derivative were utilized with the aim of investigating the changes in the porous system. Pore size distribution was also evaluated. The experiment was carried out at a greenhouse and the soil water content for the different water regimes was monitored by means of a TDR. Four undisturbed samples were collected from each wooden bed (eight) for the physic-hydrical characterization. Discrepancies in the SWRC were noticed for the region of small pressure heads.Differenceswere not observed between bean cultivars to SWRC. However, the water capacity function was sensitive to show differences in the soil porous system due to the treatments and cultivars. The lowest water regimes promoted the highest volume of fissures (big pores >250 µm) and, consequently, the highest ones had the largest volume of storage pores (<25 µm).
Agricultural Engineering International: The CIGR Journal, 2018
Beans crop is an agricultural staple largely consumed in Brazil because it is part of the basic f... more Beans crop is an agricultural staple largely consumed in Brazil because it is part of the basic food of the population. Such a crop is usually grown by small growers and turns out to be of a great social and economical importance. The aim of the current manuscript was to scrutinize the effect of soil water status on its physical attributes, such as bulk density, total porosity, macroporosity, microporosity, hydraulic conductivity under protected environment conditions on beans crop ( Phaseolus vulgaris L.). The effect of the soil physical attributes on the biological response of the plants was also investigated. The experiment was installed in March of 2016 in a greenhouse. The experimental design was completely randomized in a factorial scheme with four replications. The soil moisture within the stipulated levels (14, 21, 28 and 35% at a volume basis) was monitored by a TDR. Bulk density and microporosity did show an increasing linear trend as a function of the soil moisture levels...
Animal Reproduction Science, 2021
The side of ovulation (left ovary, LO; right ovary, RO) and side of the next ovulation were compa... more The side of ovulation (left ovary, LO; right ovary, RO) and side of the next ovulation were compared between (1) beginning and end of an interovulatory interval (IOI) and beginning and end of consecutive sets of two and three IOI (n = 900 IOI), (2) beginning and end of the IOI for two and three follicular waves per IOI (n = 1300), and (3) beginning of pregnancy and first postpartum ovulation (n = 793). Pairs of sides of ovulation were designated LL (LO and LO), RR, LR, and RL. The frequency of ovulation pairs for two ends of an IOI was not different from two ends of two or three consecutive IOI indicating that differences between LO and RO were more likely inherent than from factors that developed in each IOI. For each end of an IOI or two consecutive IOI, the least frequency (P < 0.05) was for LL (16 %) with no differences among RR, LR, and RL (28 % for each). Frequencies between ipsilateral (LL, RR) and contralateral (LR, RL) ovulations pairs were not different for two-wave IOI (48 % compared with 52 %) but differed (P < 0.0001) for three-wave IOI (32 % compared with 68 %) and for pregnancy/postpartum (34 % compared with 66 %). In pregnancy/postpartum, each pair was different (P < 0.05) from each other: LL (13 %), RR (21 %), LR (30 %), RL (36 %). The lesser frequency for LL than for any of the others for an IOI, consecutive IOI, and pregnancy/postpartum indicated a ubiquity of the small propensity for LO ovulation.
Revista Encontros Teológicos, 2018
Com o advento do Ano do Laicato no Brasil, a Igreja é chamada avalorizar esta que é a maior parce... more Com o advento do Ano do Laicato no Brasil, a Igreja é chamada avalorizar esta que é a maior parcela dos cristãos católicos. O Concílio VaticanoII menciona diversas vezes, em documentos variados acerca da dignidadevocacional dos leigos, sobre sua identidade, acenando igualmente para seuapostolado ad intra e ad extra da própria Igreja. É alvitrado que os leigos e leigasse sintam e sejam efetivamente sujeitos eclesiais, corresponsáveis pela missãoda Igreja. E dentro do conjunto laical, maior atenção é conferida às mulheres,por vários motivos que justificam o reconhecimento do laicato participativo,sobretudo do laicato feminino, que necessita ser mais valorizado, contrapondouma sociedade assinalada pelo patriarcalismo exacerbado. Para tal, a partir daCarta Apostólica Mulieris Dignitatem do Papa João Paulo II, intenta-se mostraras razões teológicas, bíblicas e pastorais pertinentes à devida deferência paracom o laicato feminino.Palavras-chave: Laicato. Mulheres. Pastoral. : With the adve...
Reproduction, 2020
Previous research demonstrated that acute treatment with GnRH antagonist, Acyline, allowed follic... more Previous research demonstrated that acute treatment with GnRH antagonist, Acyline, allowed follicle growth until ~8.5 mm and no dominant follicle was selected. This study evaluated whether deficient LH was the underlying mechanism for Acyline effects by replacing LH action, using human chorionic gonadotropin (hCG), during Acyline treatment. Holstein heifers (n = 24) during first follicular wave were evaluated by ultrasound and randomized into one of three treatments: Control (saline treatments), Acyline (5 µg/kg Acyline), or Acyline+hCG (Acyline plus 50 IU of hCG at start then 100 IU every 12 h). Pulses of LH were present in Control heifers (9 Pulses/10 h) but not during Acyline treatment. Data were normalized to the transition to diameter deviation (day 0; F1 ~7.5 mm). Diameter deviation of the largest (F1) and the second largest (F2) follicle was not observed in Acyline-treated heifers, whereas control heifers had decreased growth of F2 at F1 ~7.5 mm, indicating deviation. Selecti...
Revista Caminhos - Revista de Ciências da Religião, 2018
Culpa e pecado são dois termos comumente rechaçados. O presente texto imerge na psicologia para b... more Culpa e pecado são dois termos comumente rechaçados. O presente texto imerge na psicologia para buscar entender o significado da culpa, com a iluminação de Jung. Perfazendo o plano moral e antropológico, se atingirá a esfera teológica, amplificando a compreensão da culpa em relação ao pecado, num patamar espiritual, conferindo novo e positivo significado à existência humana. INTERFACES BETWEEN GUILT AND SIN Guilt and sin are two commonly rejected terms. This text immerses in psychology to seek to understand the meaning of guilt, with the enlightenment of Jung. By achieving the moral and anthropological plane, the theological sphere will be reached, amplifying the understanding of the guilt in relation to the sin, in a spiritual level, giving new and positive meaning to the human existence.
Journal of invertebrate pathology, Nov 30, 2017
Gastrointestinal nematodes (GIN) can reduce or limit sheep production. Currently there is a clear... more Gastrointestinal nematodes (GIN) can reduce or limit sheep production. Currently there is a clear deficiency in the action of drugs for the control of these parasites. Nematophagous fungi are natural enemies of GIN. Fungal combinations have potential for reducing GIN populations. The aim of this study was to evaluate the efficiency combinations of nematophagous fungi in sodium alginate matrix pellets for the biological control agents of gastrointestinal sheep nematode parasites in the field. The nematophagous fungi (0.2mg of fungus per kg of body weight), Arthrobotrys conoides, A. robusta, Duddingtonia flagrans, and Monacrosporium thaumasium were used. The treated groups were administered mycelium combinations in the following combinations: group 1 (D. flagrans+A. robusta); group 2 (M. thaumasium+A. conoides). The control group did not receive any fungal pellets. We used three groups with eight Santa Inês sheep each. Each animal was treated with approximately 1g of pellet per 10kg o...
Small Ruminant Research, 2017
Highlights Combinations of nematophagous fungi can control infective larvae of Strongiloydea ... more Highlights Combinations of nematophagous fungi can control infective larvae of Strongiloydea Conidia and chlamydospores pass through the gastrointestinal tract of goats. New option for the biological control of gastrointestinal parasites of goats.
International Journal of Advanced Engineering Research and Science, 2018
Bean (Phaseolus vulgaris L.) is a crop of great economic and social impacts in Brazil. This crop ... more Bean (Phaseolus vulgaris L.) is a crop of great economic and social impacts in Brazil. This crop is extremely appreciated by the Brazilian population and an important source of protein. Usually the small farmers are responsible by the largest production of the bean in Brazil. This work deals with the analysis of the effect of different water regimes (35, 28, 21 and 14%)on the porous system of a soil cropped with two distinct cultivars (Campos Gerais and Tuiuiú). Soil water retention curve (SWRC) and its derivative were utilized with the aim of investigating the changes in the porous system. Pore size distribution was also evaluated. The experiment was carried out at a greenhouse and the soil water content for the different water regimes was monitored by means of a TDR. Four undisturbed samples were collected from each wooden bed (eight) for the physic-hydrical characterization. Discrepancies in the SWRC were noticed for the region of small pressure heads.Differenceswere not observed between bean cultivars to SWRC. However, the water capacity function was sensitive to show differences in the soil porous system due to the treatments and cultivars. The lowest water regimes promoted the highest volume of fissures (big pores >250 µm) and, consequently, the highest ones had the largest volume of storage pores (<25 µm).
Agricultural Engineering International: The CIGR Journal, 2018
Beans crop is an agricultural staple largely consumed in Brazil because it is part of the basic f... more Beans crop is an agricultural staple largely consumed in Brazil because it is part of the basic food of the population. Such a crop is usually grown by small growers and turns out to be of a great social and economical importance. The aim of the current manuscript was to scrutinize the effect of soil water status on its physical attributes, such as bulk density, total porosity, macroporosity, microporosity, hydraulic conductivity under protected environment conditions on beans crop ( Phaseolus vulgaris L.). The effect of the soil physical attributes on the biological response of the plants was also investigated. The experiment was installed in March of 2016 in a greenhouse. The experimental design was completely randomized in a factorial scheme with four replications. The soil moisture within the stipulated levels (14, 21, 28 and 35% at a volume basis) was monitored by a TDR. Bulk density and microporosity did show an increasing linear trend as a function of the soil moisture levels...
Journal of Basic Microbiology, 2013
Research involving the use of nematophagous fungi in the biological control of parasites of inter... more Research involving the use of nematophagous fungi in the biological control of parasites of interest to veterinarians has occurred over recent years, with promising results. This article reports the infection of Parascaris equorum eggs by the fungus Pochonia chlamydosporia (isolates VC1 and VC4). Six groups were formed for each isolate, with six different culture media: 2% wateragar (2% WA); agar-chitin (AC); YPSSA (yeast extract, K 2 HPO 4 , MgSO 4 •7H 2 O, soluble starch); AELA extract (starch þ water þ agar); 2% corn-meal-agar (2% CMA); and 2% potato dextrose-agar (2% PDA). A total of 1000 eggs of P. equorum were transferred to each plate containing isolates grown for a period of 7 days (treatment group). Also, 1000 eggs were added to each plate without fungus (controlgroup). The plates were kept in an environmental chamber at 25°C in the dark for 21 days. After, we analyzed the effects on ovicidal activity: effect 1 (accession shell); effect 2 (penetration hyphae); and effect 3 (destruction of the eggs). No differences were observed in the destruction of eggs between the two isolates. The decreasing effectiveness of the different culture media was: PDA (38.9%); CMA (38.3%); WA (36.7%); YPSSA (36.45%); and AC (32.5%). The highest percentage egg destruction was observed when the strains were grown in culture medium AELA (44.9%); this was the best medium.
Agricultural Engineering International: The CIGR Journal, 2017
Climatic conditions and soil water requirement notably impinge upon agriculture in such a way as ... more Climatic conditions and soil water requirement notably impinge upon agriculture in such a way as to galvanize scientists to come up with advanced research in order to assure a more sustainable production at a given site. Under protected environmental conditions water is the main factor to be controlled aiming at maximization of yield and grade of agricultural products. The productive potential of a crop is extremely conditioned by the local atmospheric conditions along with requirements for water and nutrients in the soil. The aim of the current work was to scrutinize the effect of soil water status on the ecophysiological parameters and yield components of two cultivars of beans ( Phaseolus vulgaris L.) under tropical climatic conditions. The experiment was carried out in March of 2016 at a greenhouse belonging to the Agronomic Institute of Parana (Ponta Grossa, PR, Brazil) with beans. The experimental design taken into account herein was a completely randomized design at a factori...
Frontiers in Medicine
Selective serotonin reuptake inhibitors (SSRI) are the most common antidepressants used by pregna... more Selective serotonin reuptake inhibitors (SSRI) are the most common antidepressants used by pregnant women. However, adverse pregnancy outcomes have been described in women taking SSRI during pregnancy—placental lesions, premature birth, poor neonatal adaptation. We aimed to investigate the effects of fluoxetine (Prozac® most commonly used SSRI) treatment during the last month of gestation on pregnancy complications, placental and neonatal health in a non-depressed sheep model. On day 119 ± 1 postbreeding (experimental day 0; E0) of a 151-day expected gestation, Hampshire ewes were randomly assigned to receive fluoxetine (n = 9 ewes, 15 lambs; daily intravenously treatment with 10 mg/kg on E0 and E1 and 5 mg/kg daily thereafter until parturition) or to a control group (n = 10; 14 lambs; vehicle only). Blood samples from ewes were collected throughout the experimental period and postpartum; blood from lambs were collected postpartum. Analysis of variance was used for statistical analy...
Reproduction, 2020
Understanding the impacts of nutrition on reproductive physiology in cattle are fundamental to im... more Understanding the impacts of nutrition on reproductive physiology in cattle are fundamental to improve reproductive efficiency for animals under different nutritional conditions. Starting on Day 0 (day of ovulation) until next ovulation, Holstein heifers (n = 24) were fed: low energy diet (ad libitum grass hay; LED) and high energy diet (ad libitum grass hay + concentrate supplement; HED). Heifers on HED gained more weight (average daily gain: 0.824 ± 0.07 vs 0.598 ± 0.09 kg/day) and had increased insulin concentrations. The dominant follicle of wave 1 in HED had greater growth rate overall from Days 0 to 8 and on Days 6–7 and 8–9 and started atresia later. The dominant follicle of wave 2 in HED had greater growth rate overall from Day 9 to 18 and on Days 14–15 and 15–16. In two-wave patterns, there was no difference in estradiol or progesterone concentrations but concentrations of FSH were lower in HED on Days 15 and 16. Estradiol concentrations increased earlier in two-wave patter...
Journal of Dairy Science, 2022
Our objective was to determine the effect of inducing an accessory corpus luteum (CL) with human ... more Our objective was to determine the effect of inducing an accessory corpus luteum (CL) with human chorionic gonadotropin (hCG; 3,300 IU) on d 7 (hCG7) or 2 accessory CL with hCG on d 7 and 13 (hCG7+13) of the estrous cycle in noninseminated lactating Holstein cows. Cows (n = 86) between 39 and 64 DIM were pretreated with an Ovsynch + CIDR protocol, and only synchronized cows were used (n = 64). The day of the last GnRH of Ovsynch was considered d 0 of the estrous cycle. Follicular and luteal dynamics of cows were evaluated daily during an entire estrous cycle by ovarian ultrasonography. Blood samples were collected daily to measure serum concentration of progesterone (P4). Cows were randomly assigned to CON (n = 22, no treatment), hCG7 (n = 20), or hCG7+13 (n = 22) treatments. Two cows from hCG7+13 failed to ovulate after hCG and were removed from the analyses post-hCG treatment. The first day of luteolysis was considered the day that P4 declined to more than 2 SD of the mean for the 4 consecutive P4 concentrations with the greatest mean in late diestrus for each individual cow. The P4 cut-off for complete luteolysis was <1.0 ng/mL. Mean P4 on d 7 (3.23 ± 0.16 ng/mL) did not differ among treatments. Cows treated with hCG had greater total luteal and original CL volume and serum P4 during diestrus than CON. Cows treated with hCG7+13 had greater serum P4 after d 13 of the cycle than hCG7. Cycles were classified as having atypical cycles if the dominant follicle or future dominant follicle at the time of luteolysis did not ovulate (delayed ovulation; CON, n = 2; hCG7, n = 4; hCG7+13, n = 3), had a short cycle (CON, n = 1), delayed (CON, n = 2) or incomplete luteolysis (CON, n = 1; hCG7, n = 4; hCG7+13, n = 5). The remainder of cycles with normal complete luteolysis followed by ovulation were considered to be typical. Based on blood perfusion, the CON cow with incomplete luteolysis had 2 original CL remaining functional after first onset of luteolysis. The rest of the cows with incomplete luteolysis (9/10) had one or more CL regressing and at least one remaining functional after first onset of luteolysis. No specific pattern for CL side (ipsilateral vs. contralateral to a CL with complete regression) was observed for nonregressed CL. Cows with incomplete luteolysis had a second onset of luteolysis to undergo complete functional luteolysis. The proportion of cows with typical cycle was 73% (16/22) for CON, 60% (12/20) for hCG7, and 55% (11/20) for hCG7+13. Cows with typical cycles treated with hCG (hCG7 and hCG7+13) had a later onset of luteolysis, prolonged time to undergo complete luteolysis, and greater proportion of cows with 3 follicular waves than CON, resulting in a longer interovulatory interval for hCG7 and hCG7+13 than CON. In summary, accessory CL induced by hCG during diestrus not only altered follicular and luteal dynamics but also deferred and prolonged the luteolytic process.
Biology of Reproduction, 2022
In heifers and mares, multiple pulses of prostaglandin F2alpha (PGF) are generally associated wit... more In heifers and mares, multiple pulses of prostaglandin F2alpha (PGF) are generally associated with complete luteal regression. Although PGF pulses occur before and during luteolysis, little is known about the role of minor PGF pulses during preluteolysis on subsequent luteal and endometrial PGF production that may initiate luteolysis. Heifers (n = 7/group) and mares (n = 6/group) were treated with a single minor dose of PGF (3.0 and 0.5 mg, respectively) during mid-luteal phase (12 and 10 days postovulation respectively). After treatment, a transient decrease in progesterone (P4) concentrations occurred in heifers between Hours 0 and 2 but at Hour 4 P4 was not different from pretreatment. In mares, P4 was unaltered between Hours 0 and 4. Concentrations of P4 decreased in both species by Hour 24 and complete luteolysis occurred in mares by Hour 48. Luteal and endometrial gene expression were evaluated 4 h posttreatment. In heifers, luteal mRNA abundance of PGF receptor and PGF dehydr...
Toxics, 2022
Selective serotonin reuptake inhibitors (SSRI) are the most common antidepressant used by pregnan... more Selective serotonin reuptake inhibitors (SSRI) are the most common antidepressant used by pregnant women; however, they have been associated with adverse pregnancy outcomes and perinatal morbidity in pregnant women and animal models. We investigated the effects of two SSRI, fluoxetine and sertraline, on pregnancy and neonatal outcomes in mice. Wild-type mice were treated daily with low and high doses of fluoxetine (2 and 20 mg/kg) and sertraline (10 and 20 mg/kg) from the day of detection of a vaginal plug until the end of lactation (21 days postpartum). Pregnancy rate was decreased only in the high dose of fluoxetine group. Maternal weight gain was reduced in the groups receiving the high dose of each drug. Number of pups born was decreased in the high dose of fluoxetine and low and high doses of sertraline while the number of pups weaned was decreased in all SSRI-treated groups corresponding to increased neonatal mortality in all SSRI-treated groups. In conclusion, there was a dos...
International Journal of Advanced Engineering Research and Science, 2018
Bean (Phaseolus vulgaris L.) is a crop of great economic and social impacts in Brazil. This crop ... more Bean (Phaseolus vulgaris L.) is a crop of great economic and social impacts in Brazil. This crop is extremely appreciated by the Brazilian population and an important source of protein. Usually the small farmers are responsible by the largest production of the bean in Brazil. This work deals with the analysis of the effect of different water regimes (35, 28, 21 and 14%)on the porous system of a soil cropped with two distinct cultivars (Campos Gerais and Tuiuiú). Soil water retention curve (SWRC) and its derivative were utilized with the aim of investigating the changes in the porous system. Pore size distribution was also evaluated. The experiment was carried out at a greenhouse and the soil water content for the different water regimes was monitored by means of a TDR. Four undisturbed samples were collected from each wooden bed (eight) for the physic-hydrical characterization. Discrepancies in the SWRC were noticed for the region of small pressure heads.Differenceswere not observed between bean cultivars to SWRC. However, the water capacity function was sensitive to show differences in the soil porous system due to the treatments and cultivars. The lowest water regimes promoted the highest volume of fissures (big pores >250 µm) and, consequently, the highest ones had the largest volume of storage pores (<25 µm).
Agricultural Engineering International: The CIGR Journal, 2018
Beans crop is an agricultural staple largely consumed in Brazil because it is part of the basic f... more Beans crop is an agricultural staple largely consumed in Brazil because it is part of the basic food of the population. Such a crop is usually grown by small growers and turns out to be of a great social and economical importance. The aim of the current manuscript was to scrutinize the effect of soil water status on its physical attributes, such as bulk density, total porosity, macroporosity, microporosity, hydraulic conductivity under protected environment conditions on beans crop ( Phaseolus vulgaris L.). The effect of the soil physical attributes on the biological response of the plants was also investigated. The experiment was installed in March of 2016 in a greenhouse. The experimental design was completely randomized in a factorial scheme with four replications. The soil moisture within the stipulated levels (14, 21, 28 and 35% at a volume basis) was monitored by a TDR. Bulk density and microporosity did show an increasing linear trend as a function of the soil moisture levels...
Animal Reproduction Science, 2021
The side of ovulation (left ovary, LO; right ovary, RO) and side of the next ovulation were compa... more The side of ovulation (left ovary, LO; right ovary, RO) and side of the next ovulation were compared between (1) beginning and end of an interovulatory interval (IOI) and beginning and end of consecutive sets of two and three IOI (n = 900 IOI), (2) beginning and end of the IOI for two and three follicular waves per IOI (n = 1300), and (3) beginning of pregnancy and first postpartum ovulation (n = 793). Pairs of sides of ovulation were designated LL (LO and LO), RR, LR, and RL. The frequency of ovulation pairs for two ends of an IOI was not different from two ends of two or three consecutive IOI indicating that differences between LO and RO were more likely inherent than from factors that developed in each IOI. For each end of an IOI or two consecutive IOI, the least frequency (P < 0.05) was for LL (16 %) with no differences among RR, LR, and RL (28 % for each). Frequencies between ipsilateral (LL, RR) and contralateral (LR, RL) ovulations pairs were not different for two-wave IOI (48 % compared with 52 %) but differed (P < 0.0001) for three-wave IOI (32 % compared with 68 %) and for pregnancy/postpartum (34 % compared with 66 %). In pregnancy/postpartum, each pair was different (P < 0.05) from each other: LL (13 %), RR (21 %), LR (30 %), RL (36 %). The lesser frequency for LL than for any of the others for an IOI, consecutive IOI, and pregnancy/postpartum indicated a ubiquity of the small propensity for LO ovulation.
Revista Encontros Teológicos, 2018
Com o advento do Ano do Laicato no Brasil, a Igreja é chamada avalorizar esta que é a maior parce... more Com o advento do Ano do Laicato no Brasil, a Igreja é chamada avalorizar esta que é a maior parcela dos cristãos católicos. O Concílio VaticanoII menciona diversas vezes, em documentos variados acerca da dignidadevocacional dos leigos, sobre sua identidade, acenando igualmente para seuapostolado ad intra e ad extra da própria Igreja. É alvitrado que os leigos e leigasse sintam e sejam efetivamente sujeitos eclesiais, corresponsáveis pela missãoda Igreja. E dentro do conjunto laical, maior atenção é conferida às mulheres,por vários motivos que justificam o reconhecimento do laicato participativo,sobretudo do laicato feminino, que necessita ser mais valorizado, contrapondouma sociedade assinalada pelo patriarcalismo exacerbado. Para tal, a partir daCarta Apostólica Mulieris Dignitatem do Papa João Paulo II, intenta-se mostraras razões teológicas, bíblicas e pastorais pertinentes à devida deferência paracom o laicato feminino.Palavras-chave: Laicato. Mulheres. Pastoral. : With the adve...
Reproduction, 2020
Previous research demonstrated that acute treatment with GnRH antagonist, Acyline, allowed follic... more Previous research demonstrated that acute treatment with GnRH antagonist, Acyline, allowed follicle growth until ~8.5 mm and no dominant follicle was selected. This study evaluated whether deficient LH was the underlying mechanism for Acyline effects by replacing LH action, using human chorionic gonadotropin (hCG), during Acyline treatment. Holstein heifers (n = 24) during first follicular wave were evaluated by ultrasound and randomized into one of three treatments: Control (saline treatments), Acyline (5 µg/kg Acyline), or Acyline+hCG (Acyline plus 50 IU of hCG at start then 100 IU every 12 h). Pulses of LH were present in Control heifers (9 Pulses/10 h) but not during Acyline treatment. Data were normalized to the transition to diameter deviation (day 0; F1 ~7.5 mm). Diameter deviation of the largest (F1) and the second largest (F2) follicle was not observed in Acyline-treated heifers, whereas control heifers had decreased growth of F2 at F1 ~7.5 mm, indicating deviation. Selecti...
Revista Caminhos - Revista de Ciências da Religião, 2018
Culpa e pecado são dois termos comumente rechaçados. O presente texto imerge na psicologia para b... more Culpa e pecado são dois termos comumente rechaçados. O presente texto imerge na psicologia para buscar entender o significado da culpa, com a iluminação de Jung. Perfazendo o plano moral e antropológico, se atingirá a esfera teológica, amplificando a compreensão da culpa em relação ao pecado, num patamar espiritual, conferindo novo e positivo significado à existência humana. INTERFACES BETWEEN GUILT AND SIN Guilt and sin are two commonly rejected terms. This text immerses in psychology to seek to understand the meaning of guilt, with the enlightenment of Jung. By achieving the moral and anthropological plane, the theological sphere will be reached, amplifying the understanding of the guilt in relation to the sin, in a spiritual level, giving new and positive meaning to the human existence.
Journal of invertebrate pathology, Nov 30, 2017
Gastrointestinal nematodes (GIN) can reduce or limit sheep production. Currently there is a clear... more Gastrointestinal nematodes (GIN) can reduce or limit sheep production. Currently there is a clear deficiency in the action of drugs for the control of these parasites. Nematophagous fungi are natural enemies of GIN. Fungal combinations have potential for reducing GIN populations. The aim of this study was to evaluate the efficiency combinations of nematophagous fungi in sodium alginate matrix pellets for the biological control agents of gastrointestinal sheep nematode parasites in the field. The nematophagous fungi (0.2mg of fungus per kg of body weight), Arthrobotrys conoides, A. robusta, Duddingtonia flagrans, and Monacrosporium thaumasium were used. The treated groups were administered mycelium combinations in the following combinations: group 1 (D. flagrans+A. robusta); group 2 (M. thaumasium+A. conoides). The control group did not receive any fungal pellets. We used three groups with eight Santa Inês sheep each. Each animal was treated with approximately 1g of pellet per 10kg o...
Small Ruminant Research, 2017
Highlights Combinations of nematophagous fungi can control infective larvae of Strongiloydea ... more Highlights Combinations of nematophagous fungi can control infective larvae of Strongiloydea Conidia and chlamydospores pass through the gastrointestinal tract of goats. New option for the biological control of gastrointestinal parasites of goats.
International Journal of Advanced Engineering Research and Science, 2018
Bean (Phaseolus vulgaris L.) is a crop of great economic and social impacts in Brazil. This crop ... more Bean (Phaseolus vulgaris L.) is a crop of great economic and social impacts in Brazil. This crop is extremely appreciated by the Brazilian population and an important source of protein. Usually the small farmers are responsible by the largest production of the bean in Brazil. This work deals with the analysis of the effect of different water regimes (35, 28, 21 and 14%)on the porous system of a soil cropped with two distinct cultivars (Campos Gerais and Tuiuiú). Soil water retention curve (SWRC) and its derivative were utilized with the aim of investigating the changes in the porous system. Pore size distribution was also evaluated. The experiment was carried out at a greenhouse and the soil water content for the different water regimes was monitored by means of a TDR. Four undisturbed samples were collected from each wooden bed (eight) for the physic-hydrical characterization. Discrepancies in the SWRC were noticed for the region of small pressure heads.Differenceswere not observed between bean cultivars to SWRC. However, the water capacity function was sensitive to show differences in the soil porous system due to the treatments and cultivars. The lowest water regimes promoted the highest volume of fissures (big pores >250 µm) and, consequently, the highest ones had the largest volume of storage pores (<25 µm).
Agricultural Engineering International: The CIGR Journal, 2018
Beans crop is an agricultural staple largely consumed in Brazil because it is part of the basic f... more Beans crop is an agricultural staple largely consumed in Brazil because it is part of the basic food of the population. Such a crop is usually grown by small growers and turns out to be of a great social and economical importance. The aim of the current manuscript was to scrutinize the effect of soil water status on its physical attributes, such as bulk density, total porosity, macroporosity, microporosity, hydraulic conductivity under protected environment conditions on beans crop ( Phaseolus vulgaris L.). The effect of the soil physical attributes on the biological response of the plants was also investigated. The experiment was installed in March of 2016 in a greenhouse. The experimental design was completely randomized in a factorial scheme with four replications. The soil moisture within the stipulated levels (14, 21, 28 and 35% at a volume basis) was monitored by a TDR. Bulk density and microporosity did show an increasing linear trend as a function of the soil moisture levels...
Journal of Basic Microbiology, 2013
Research involving the use of nematophagous fungi in the biological control of parasites of inter... more Research involving the use of nematophagous fungi in the biological control of parasites of interest to veterinarians has occurred over recent years, with promising results. This article reports the infection of Parascaris equorum eggs by the fungus Pochonia chlamydosporia (isolates VC1 and VC4). Six groups were formed for each isolate, with six different culture media: 2% wateragar (2% WA); agar-chitin (AC); YPSSA (yeast extract, K 2 HPO 4 , MgSO 4 •7H 2 O, soluble starch); AELA extract (starch þ water þ agar); 2% corn-meal-agar (2% CMA); and 2% potato dextrose-agar (2% PDA). A total of 1000 eggs of P. equorum were transferred to each plate containing isolates grown for a period of 7 days (treatment group). Also, 1000 eggs were added to each plate without fungus (controlgroup). The plates were kept in an environmental chamber at 25°C in the dark for 21 days. After, we analyzed the effects on ovicidal activity: effect 1 (accession shell); effect 2 (penetration hyphae); and effect 3 (destruction of the eggs). No differences were observed in the destruction of eggs between the two isolates. The decreasing effectiveness of the different culture media was: PDA (38.9%); CMA (38.3%); WA (36.7%); YPSSA (36.45%); and AC (32.5%). The highest percentage egg destruction was observed when the strains were grown in culture medium AELA (44.9%); this was the best medium.
Agricultural Engineering International: The CIGR Journal, 2017
Climatic conditions and soil water requirement notably impinge upon agriculture in such a way as ... more Climatic conditions and soil water requirement notably impinge upon agriculture in such a way as to galvanize scientists to come up with advanced research in order to assure a more sustainable production at a given site. Under protected environmental conditions water is the main factor to be controlled aiming at maximization of yield and grade of agricultural products. The productive potential of a crop is extremely conditioned by the local atmospheric conditions along with requirements for water and nutrients in the soil. The aim of the current work was to scrutinize the effect of soil water status on the ecophysiological parameters and yield components of two cultivars of beans ( Phaseolus vulgaris L.) under tropical climatic conditions. The experiment was carried out in March of 2016 at a greenhouse belonging to the Agronomic Institute of Parana (Ponta Grossa, PR, Brazil) with beans. The experimental design taken into account herein was a completely randomized design at a factori...