Raha Rad - Academia.edu (original) (raw)

Papers by Raha Rad

BIOFACTORS- …, Jan 1, 1998

... BH Robinson, XP Luo, S. Pitkänen, S. Bratinova, J. Bourgeois, DC Lehotay and S. Raha Universi... more ... BH Robinson, XP Luo, S. Pitkänen, S. Bratinova, J. Bourgeois, DC Lehotay and S. Raha University of Toronto and Research Institute, Hospital for ... JR Trudell, C. Altenbach, DJ Kane, WL Hubell and DE Bredesn, Expression of bcl-2 inhibits cellular radical generation, Free Rad. ...

PloS one, Jan 1, 2009

Advancements in animal models and cell culture techniques have been invaluable in the elucidation... more Advancements in animal models and cell culture techniques have been invaluable in the elucidation of the molecular mechanisms that regulate muscle atrophy. However, few studies have examined muscle atrophy in humans using modern experimental techniques. The purpose of this study was to examine changes in global gene transcription during immobilization-induced muscle atrophy in humans and then explore the effects of the most prominent transcriptional alterations on protein expression and function. Healthy men and women (N = 24) were subjected to two weeks of unilateral limb immobilization, with muscle biopsies obtained before, after 48 hours (48 H) and 14 days (14 D) of immobilization. Muscle cross sectional area (,5%) and strength (10-20%) were significantly reduced in men and women (,5% and 10-20%, respectively) after 14 D of immobilization. Micro-array analyses of total RNA extracted from biopsy samples at 48 H and 14 D uncovered 575 and 3,128 probes, respectively, which were significantly altered during immobilization. As a group, genes involved in mitochondrial bioenergetics and carbohydrate metabolism were predominant features at both 48 H and 14 D, with genes involved in protein synthesis and degradation significantly down-regulated and up-regulated, respectively, at 14 D of muscle atrophy. There was also a significant decrease in the protein content of mitochondrial cytochrome c oxidase, and the enzyme activity of cytochrome c oxidase and citrate synthase after 14 D of immobilization. Furthermore, protein ubiquitination was significantly increased at 48 H but not 14 D of immobilization. These results suggest that transcriptional and post-transcriptional suppression of mitochondrial processes is sustained throughout 14 D of immobilization, while protein ubiquitination plays an early but transient role in muscle atrophy following short-term immobilization in humans.

Gene, Jan 1, 2011

MicroRNAs (miRNAs) are small non-coding RNA molecules that play a vital role in the regulation of... more MicroRNAs (miRNAs) are small non-coding RNA molecules that play a vital role in the regulation of gene expression. Despite their identification in hundreds of plant species, few miRNAs have been identified in the Asteraceae, a large family that comprises approximately one tenth of all flowering plants. In this study, we used the expressed sequence tag (EST) analysis to identify potential conserved miRNAs and their putative target genes in the Asteraceae. We applied quantitative Real-Time PCR (qRT-PCR) to confirm the expression of eight potential miRNAs in Carthamus tinctorius and Helianthus annuus. We also performed qRT-PCR analysis to investigate the differential expression pattern of five newly identified miRNAs during five different cotyledon growth stages in safflower. Using these methods, we successfully identified and characterized 151 potentially conserved miRNAs, belonging to 26 miRNA families, in 11 genus of Asteraceae. EST analysis predicted that the newly identified conserved Asteraceae miRNAs target 130 total protein-coding ESTs in sunflower and safflower, as well as 433 additional target genes in other plant species. We experimentally confirmed the existence of seven predicted miRNAs, (miR156, miR159, miR160, miR162, miR166, miR396, and miR398) in safflower and sunflower seedlings. We also observed that five out of eight miRNAs are differentially expressed during cotyledon development. Our results indicate that miRNAs may be involved in the regulation of gene expression during seed germination and the formation of the cotyledons in the Asteraceae. The findings of this study might ultimately help in the understanding of miRNA-mediated gene regulation in important crop species.

Polymer …, Jan 1, 2012

The investigated rheological properties of polylactic acid (PLA)/clay nanocomposite are important... more The investigated rheological properties of polylactic acid (PLA)/clay nanocomposite are important to understand the effect of organically modified layered silicates (OMLS) (clay) on processing as well as the change in viscoelastic properties due to polymer filler interaction. The time sweep result revealed that the thermal stability improved with addition of nanoclay due to the formation of percolating network structure. It was also supported by multi wave ramp test. The frequency sweep analysis showed that the dynamic moduli increased with addition of nanoclay. Viscoelastic spectra (DMTA) showed an increase of the storage and loss moduli with the increase in the clay content. Wide angle X-ray diffraction (WAXD) and transmission electron microscopy (TEM) were used to determine the degree of intercalation, or exfoliation and nanostructure level of clay dispersion on PLA nanocomposites. XRD data demonstrated complete exfoliation at lower nanoclay content. On increasing the nanoclay content, exfoliated and partially intercalated structures were obtained. POLYM. ENG. SCI.,

African Journal of …, Jan 1, 2010

PrIFN-α2b) by recombinant Escherichia coli was conducted using shake flask culture. Recombinant E... more PrIFN-α2b) by recombinant Escherichia coli was conducted using shake flask culture. Recombinant E. coli Rosetta-gami 2(DE3) harboring the plasmid pET26b containing IFN-α2b gene under the control of the T7lac promoter was used, where the induction was accomplished by isopropyl β-D-1thiogalactopyranoside (IPTG). The induction parameters (inducer concentration, point of induction, induction temperature and the length of induction) were analyzed to find the suitable range to be used for further optimization process. From the analysis, narrow range of induction temperature from 16 to 30°C and IPTG lower than 2 mM were found suitable for induction of PrIFN-α2b. On the other hand, early log phase was the preferred time to initiate the induction and the length of induction was dependent on the combination of other induction parameters used.

Biochemical …, Jan 1, 2011

The user has requested enhancement of the downloaded file. All in-text references underlined in b... more The user has requested enhancement of the downloaded file. All in-text references underlined in blue are added to the original document and are linked to publications on ResearchGate, letting you access and read them immediately. This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution and sharing with colleagues.

FEBS letters, Jan 1, 1996

Exposure of HeLa cells in monolayer culture to increasing concentrations of exogenously added H 2... more Exposure of HeLa cells in monolayer culture to increasing concentrations of exogenously added H 2 O 2 causes damage to cellular DNA. When the DNA is subsequently isolated from the non-apoptotic cells remaining in such cultures, evidence was obtained to suggest that the ...

Journal of Agricultural …, Jan 1, 1982

... Sidney S. Mirvish,* David A. Cairnes,l Nadwa H. Hermes, and Chitta R. Raha ... To do this, th... more ... Sidney S. Mirvish,* David A. Cairnes,l Nadwa H. Hermes, and Chitta R. Raha ... To do this, the solution was adjusted to pH 1 and applied to a column of Bio-Rad AG-50W-X8 cation-exchange resin (100-200 mesh, 2.3-cm diameter, 25-cm height, H+ form, Bio-Rad Laboratories ...

The Journal of …, Jan 1, 2006