ramanuj maurya - Academia.edu (original) (raw)

Papers by ramanuj maurya

Imperial journal of interdisciplinary research, 2016

Climate change is now major global problem in the area of agriculture as rising food demands from... more Climate change is now major global problem in the area of agriculture as rising food demands from a larger and wealthier human population. These factors point to a global food deficit unless the efficiency and resilience of crop production is increased. The intensification of agriculture has focused on improving production under optimized conditions, with significant agronomic inputs. A new agricultural paradigm is required, reducing dependence on high inputs and increasing crop diversity, yield stability and environmental resilience. In the last few years there has been rapid progress in the area of plant structural and functional genomics. Next-generation DNA sequencing technologies which permit whole-genome and transcriptome sequencing are relatively inexpensive and accessible for many research groups. Genomics offers great opportunities to increase crop yield, quality and stability of production through advance breeding strategies, enhancing the resilience of major crops to cli...

Tropical Plant Research, 2016

Combining ability analysis was studied in a 10 x 10 diallele set of bread to understand the inher... more Combining ability analysis was studied in a 10 x 10 diallele set of bread to understand the inheritance pattern of several yield and quality traits in bread wheat. Results showed that the GCA variances were higher than SCA variances for plant height, spike length, duration of reproductive phase, gluten content and grain yield per plant in both the generations. General predictability ratio (GPR) did not reach near to unity for any of the traits in both the generations except tryptophan content in F1 generation. On the basis of overall performance, parents K8962, K9423, PBW343, GW373, HD2733 and Sonalika were best general combiners for grain yield and other important characters. Among the cross significant and desirable SCA effects in order of merit for yield and yield contributing traits were exhibited by GW373/K8962, K9107/K8027, GW373/Sonalika, K8027/K8962, K8962/Sonalika, K9107/K9423, K8020/K9423, K9107/K9351, K9107/K8962, K9107/Sonalika. These parents may be used for simultaneous...

The tree breeding is more difficult by the changes that occur during the transition from juvenili... more The tree breeding is more difficult by the changes that occur during the transition from juvenility to maturity. A correlation between individual heterozygosities of parents and their offspring arises from the fact that, at most allelic frequencies, heterozygous parents produce higher proportion of heterozygous progeny than do homozygous parents. Microsatellite markers are an efficient tool for the assessment of heterozygosity and homozygosity. In order to assess the level of heterozygosity of Jatropha curcas, 56 SSRs markers were used for genotyping 48 progeny derived from selfed seeds of a single J. curcas plant. Out of 56, 7 SSRs could not produce sufficient and significant data as they failed to amplify in more than 70% genotypes and thus not considered for further analysis. Therefore, genotypic data of 49 SSRs were used for heterozygosity assessment. Out of 49 SSRs, 31 SSRs were found to be monomorphic and 18 polymorphic indicating homozygosity and heterozygosity on plants, res...

Tropical Plant Research

The physiocochemical properties of Jatropha curcas kernel oils were characterized as potential bi... more The physiocochemical properties of Jatropha curcas kernel oils were characterized as potential biodiesel, including oil yield per plant, seed oil content, kernel oil content, acid value, iodine value, saponification value and cetane number. Twenty-five accessions of Jatropha curcas were used for oil content measurement sranging from 21.14 to 40.66 %with a mean value of 32.85% and Kernels oil 48.59 to 60.45 % with a mean value of 56.28 %. The seed index ranged significantly from a seed weight of 45.45 to 64.45 g. Oil yields per plant ranged from 0.44 to 2.85 kg with a mean value of 1.70 kg per plant, respectively. To understand the properties of acid value, iodine value, saponification and cetane number, experimental physio-chemical studies were performed. Since these properties are critical for determining the current oil condition. The current study confirms that accession seeds performed higher than international saponification value, iodine value and cetane number standards may b...

Cereal Grains [Working Title]

Rice is the predominant crop in India and is the staple food in eastern and southern Indian popul... more Rice is the predominant crop in India and is the staple food in eastern and southern Indian populations. One of the oldest grown crops is rice. The initial discovery of cytoplasmic male sterile (CMS) three-line system made it possible to produce hybrids that significantly increase rice yields compared to its inbred counterparts. Further genetic and molecular studies help elucidate the mechanisms involved in CMS male sterility. Additional CMS types were also discovered with similar genetic control from wild sources by interspecific hybridization. In India more than 1200 varieties were released for cultivation suitable different ecosystems and out of them 128 varieties have been contributed from NRRI, Cuttack. A list of these varieties are furnished below with their duration, grain type, yield potential, reaction to major disease and insects grain quality and tolerance to different adverse situations. Recent advances in molecular approaches used in modern rice breeding include molecul...

Molecular Breeding, 2015

Abstract Wild relatives are important genetic resources for crop improvement. However, basic info... more Abstract Wild relatives are important genetic resources for crop improvement. However, basic information about their population structure, genetic diversity, species relationships and distribution of variation in a gene pool remains scanty in Vigna species. The level of genetic diversity and population genetic structure of representative accessions of cultivated and wild Asiatic Vigna species collected from diversity-rich endemic areas of India have been investigated using both microsatellite markers and morphological descriptors. Forty-one wild and 12 cultivated accessions of 13 Vigna species were genotyped using 53 polymorphic microsatellite markers. A total of 539 alleles were detected among 53 accessions at all loci with an average 10.16 alleles per locus. The major allele frequency varied from 0.16 to 0.65 (mean = 0.30), while polymorphism information content of polymorphic markers ranged from 0.47 to 0.89 (mean = 0.79). The UPGMA revealed five major clusters accommodating ~96 % of the accessions. The largest cluster accommodated 19 (36 %), while the smallest cluster had only two accessions. Two accessions, JAP/10-5 and JAP/10-9 of V. trilobata, did not group with any other accession. The model-based population structure analysis also showed almost similar pattern and grouped 53 accessions of Vigna into five genetically distinct sub-populations (K = 5) based on maximum ∆K values. Duncan’s multiple range test revealed significant difference between five genetic and one admixture group developed through population structure analysis with 22 morphological descriptors. Analysis of variance for morphological data revealed significant difference in 12 qualitative and quantitative traits including growth habit, terminal leaflet length, colour of petiole base, petiole length, leaf senescence, length of peduncle, raceme position, calyx colour, colour of VSI pod, pod pubescence, pod curvature and 100-seed weight, indicating their significance in distinguishing population groups. The information on genetic diversity and population structure of wild and cultivated accessions of Asiatic Vigna will be tremendously useful to accelerate their use in trait improvement.

Genetika, 2015

Correlation and path analysis on 80 diverse accessions of J. curcas showed that seed weight/plant... more Correlation and path analysis on 80 diverse accessions of J. curcas showed that seed weight/plant was significantly and positively associated with female flower/plant, male flower/plant, number of flower/plant, number of seed/plant, fruit weight/plant, seed width and negatively associated with oil content. Oil content was negatively and significantly correlated with all the traits studied with strong negative association with female flower/plant followed by male flower/plant, number of seed/plant, fruit weight/plant and seed weight/plant. Male flower per plant had the maximum direct effect on seed yield, followed by number of seeds/plant, seed width, number of fruits/plant and oil content. The results of the present investigations suggests that selection in J. curcas based on male flower/plant, number of fruit/plant, number of seeds/plant, seed width and oil content would be advantageous to achieve the desirable goals. The indirect selection through other component traits would also...

Gene, 2014

AFLP fingerprinting of 45 Indian genotypes of linseed was carried out to determine the genetic re... more AFLP fingerprinting of 45 Indian genotypes of linseed was carried out to determine the genetic relationship among them. Sixteen primer combinations produced 1142 fragments with 1129 as polymorphic and 13 as monomorphic fragments. Polymorphic fragments varied from 44 (E-ACA/M-CTA) to 94 (E-AGC/M-CAC) with an average of 70.6 fragments per primer combination. The frequency of polymorphism varied from 93.7% to 100% with an average of 98.8% across all the genotypes. The PIC value ranged from 0.19 to 0.31 with an average of 0.23 per primer combination. The primer pair E-AGC/M-CAC showed the maximum PIC value (0.31) followed by E-AGC/M-CAG (0.29), E-AAC/M-CAG (0.26) and E-AGC/M-CTA (0.25). Resolving power (RP) and marker index (MI) varied from 13.73 to 43.50 and 8.81 to 28.91 respectively. The Jaccard&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s similarity coefficient varied from 0.16 to 0.57 with an average of 0.26 ± 0.05. The maximum genetic similarities (57%) were detected between genotypes Him Alsi-1 and Him Alsi-2, followed by Him Alsi-1 and GS41 and GS41 and LC-54. The genotypes R-552, Himani, RKY-14, Meera, Indira Alsi-32 and Suyog were found to be more divergent genotypes. The NJ clustering grouped all the 45 genotypes into three major clusters. In general the genotypes of cluster III had high oil content and those of cluster I had low oil content. At the population level, within population variance was much higher than between populations variance.

Genetika, 2013

Genetic variability of 80 accessions of Jatropha curcas showed that oil content varied between 20... more Genetic variability of 80 accessions of Jatropha curcas showed that oil content varied between 20.8-36.1% (X=26.2?0.38). Thirty seven accessions showed seed weight/plant above average mean value (180.2g) and 26 accessions showed oil content above average mean (26.2%). The hierarchical clustering grouped all the accessions into 4 clusters. Clustering showed that majority of accessions i.e. 56 (70%) were genetically close to each other and grouped in two clusters. The maximum intra cluster distance was recorded in cluster IV (30.15). The inter cluster distance varied from 47.59 (between cluster I and cluster II) to 211.27 (between cluster III and cluster I). The cluster III showed maximum genetic distance with cluster I, followed by cluster IV and cluster II suggesting comparatively wider genetic diversity among them. The Principal Component Analysis (PCA) showed that first four principal components (PCs) accounted for more than 93% of the total variation. The first principal componen...

Scientia Horticulturae, 2013

Industrial Crops and Products, 2013

We developed and characterized 1207 SSRs to enrich the validated markers repertoire of Jatropha c... more We developed and characterized 1207 SSRs to enrich the validated markers repertoire of Jatropha curcas. A total of 248 polymorphic SSRs were identified with a panel of 7 accessions of J. curcas including some exotic accessions. Furthermore, 179 and 331 SSRs were found polymorphic among parental lines of NBRI-J05 × EC643912 and Chhatrapati × Jatropha integerrima used in developing two mapping population respectively. The number of alleles varied from 2 to 5 with an average of 2.24 ± 0.55 and 2.42 ± 0.62 alleles/SSR for CA and GA enriched library respectively. Most of the SSRs had lower PIC value (less than 0.30) and the maximum PIC value was observed for JGM A281, JGM A326 (0.63) followed by JGM B300 (0.62), JGM B361 (0.58), JGM A244 (0.55), JGM B595 (0.55) and JGM B176 (0.55). The genetic similarity coefficient among the accessions of J. curcas and one accession of J. integerrima ranged from 0.11 to 0.92 with an average of 0.57 ± 0.31. The phenogram classified all the 7 accessions of J. curcas in one cluster and the J. integerrima remained as an out group. The BLASTX analysis of SSR containing sequences showed maximum similarity of 50% with Ricinus communis (Euphorbiaceae) followed by Populus trichocarpa (22%), Vitis vinifera (16%) and Arabidopsis spp. (4.5%). This study may enrich the validated repertoire of SSR markers in J. curcas and could be used in various genetic studies including construction of linkage map, diversity analysis, and QTL/association mapping.

Plant Breeding, 2016

Mungbean (V. radiata) is an important Asiatic legume supplying inexpensive protein to a vast majo... more Mungbean (V. radiata) is an important Asiatic legume supplying inexpensive protein to a vast majority of vegetarian masses. To increase markers repertoire in mungbean, a study was conducted to analyse 384 microsatellite markers derived from common bean, scarlet runner bean and adzuki bean for their transferability and polymorphism. The results showed that 87 (24.71%) primer pairs could amplify DNA loci of 20 mungbean genotypes including one accession of V. trilobata, while 52 showed reliable banding and polymorphism. These showed different degrees of variability at each locus producing 250 alleles with the number of alleles varying from 2 to 9. The major allele frequency varied from 0.17 to 0.95, while the polymorphic information content of SSRs ranged between 0.09 and 0.86 with an average of 0.60 ± 0.16. UPGMA revealed three major clusters accommodating ~95% of the accessions while one accession of V. trilobata (‘NSB-007’) did not group with any other genotype describing the discriminating power of informative microsatellites. This study identified a set of useful microsatellite markers to accelerate the genetic studies and breeding programme of mungbean.