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Research paper thumbnail of Evaluation of RUT, and ELISA Tests for Detection of Helicobacter pylori in Dyspeptic Patients Visiting a Tertiary Care Hospital, South India

Journal of Pure and Applied Microbiology

Helicobacter pylori is main causative agent of acute gastritis and peptic ulcer disease. In certa... more Helicobacter pylori is main causative agent of acute gastritis and peptic ulcer disease. In certain population, this infection leads to gastric cancers viz., adenocarcinoma, and mucosal-associated lymphoid tissue (MALT) lymphoma. The objective of this study was to comparatively evaluate invasive (RUT) and non-invasive (IgM and IgG ELISA) methods for detection of H. pylori infection among patients visiting a tertiary care hospital. A total of 285 dyspeptic patients undergoing endoscopic examination were included in this study. From each patient one biopsy specimen and serum samples were collected. Biopsy specimen was subjected to RUT and IgM & IgG ELISA tests were performed using serum samples. H. pylori was detected in 127 (44.6%) and 126 (44.2%) cases by RUT and IgM ELISA, respectively. H. pylori were detected in 85 (29.8%) samples by IgG ELISA. Based on the combination of RUT and IgM ELISA test, total 128 (44.9%) patients were positive for H. pylori infection. Most of the positive...

Research paper thumbnail of Prevalence of dermatophytes in patients attending a teaching hospital in Hyderabad, South India

Indian Journal of Microbiology Research, 2021

Dermatophytoses are a group of superficial fungal infections of keratinized tissues. Though they ... more Dermatophytoses are a group of superficial fungal infections of keratinized tissues. Though they do not cause mortality they cause morbidity and pose a major public health problem especially in tropical countries like India. The present study is done to know the prevalence of dermatophytes and to identify the causative agents in Hyderabad area. A total of 180 samples were collected for a period of 1 year from infected skin, hair, and nails. Samples were collected under aseptic conditions and were then subjected to direct microscopy by KOH mount and culture on SDA. Dermatophytes were seen more in males (126/180). Direct microscopy by KOH mount revealed presence of fungal elements in (144/180) of samples. T.mentagrophytes was the most predominant isolate followed by T.rubrum and T.violaceum. Dermatophytic infections are worldwide in distribution with superficial mycoses being more prevalent in India where heat and moisture play an important role. Dermatophytosis has been reported thro...

Research paper thumbnail of Detection of Extended-Spectrum  -Lactamases in Members of the Family Enterobacteriaceae: Comparison of the Double-Disk and Three-Dimensional Tests

Antimicrobial Agents and Chemotherapy, 1992

Research paper thumbnail of Evaluation of RUT, and ELISA Tests for Detection of Helicobacter pylori in Dyspeptic Patients Visiting a Tertiary Care Hospital, South India

Journal of Pure and Applied Microbiology

Helicobacter pylori is main causative agent of acute gastritis and peptic ulcer disease. In certa... more Helicobacter pylori is main causative agent of acute gastritis and peptic ulcer disease. In certain population, this infection leads to gastric cancers viz., adenocarcinoma, and mucosal-associated lymphoid tissue (MALT) lymphoma. The objective of this study was to comparatively evaluate invasive (RUT) and non-invasive (IgM and IgG ELISA) methods for detection of H. pylori infection among patients visiting a tertiary care hospital. A total of 285 dyspeptic patients undergoing endoscopic examination were included in this study. From each patient one biopsy specimen and serum samples were collected. Biopsy specimen was subjected to RUT and IgM & IgG ELISA tests were performed using serum samples. H. pylori was detected in 127 (44.6%) and 126 (44.2%) cases by RUT and IgM ELISA, respectively. H. pylori were detected in 85 (29.8%) samples by IgG ELISA. Based on the combination of RUT and IgM ELISA test, total 128 (44.9%) patients were positive for H. pylori infection. Most of the positive...

Research paper thumbnail of Prevalence of dermatophytes in patients attending a teaching hospital in Hyderabad, South India

Indian Journal of Microbiology Research, 2021

Dermatophytoses are a group of superficial fungal infections of keratinized tissues. Though they ... more Dermatophytoses are a group of superficial fungal infections of keratinized tissues. Though they do not cause mortality they cause morbidity and pose a major public health problem especially in tropical countries like India. The present study is done to know the prevalence of dermatophytes and to identify the causative agents in Hyderabad area. A total of 180 samples were collected for a period of 1 year from infected skin, hair, and nails. Samples were collected under aseptic conditions and were then subjected to direct microscopy by KOH mount and culture on SDA. Dermatophytes were seen more in males (126/180). Direct microscopy by KOH mount revealed presence of fungal elements in (144/180) of samples. T.mentagrophytes was the most predominant isolate followed by T.rubrum and T.violaceum. Dermatophytic infections are worldwide in distribution with superficial mycoses being more prevalent in India where heat and moisture play an important role. Dermatophytosis has been reported thro...

Research paper thumbnail of Detection of Extended-Spectrum  -Lactamases in Members of the Family Enterobacteriaceae: Comparison of the Double-Disk and Three-Dimensional Tests

Antimicrobial Agents and Chemotherapy, 1992

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