reza soleimani - Academia.edu (original) (raw)
Papers by reza soleimani
Information
Physiological and kinematic signals from humans are often used for monitoring health. Several pro... more Physiological and kinematic signals from humans are often used for monitoring health. Several processes of interest (e.g., cardiac and respiratory processes, and locomotion) demonstrate periodicity. Training models for inference on these signals (e.g., detection of anomalies, and extraction of biomarkers) require large amounts of data to capture their variability, which are not readily available. This hinders the performance of complex inference models. In this work, we introduce a methodology for improving inference on such signals by incorporating phase-based interpretability and other inference tasks into a multi-task framework applied to a generative model. For this purpose, we utilize phase information as a regularization term and as an input to the model and introduce an interpretable unit in a neural network, which imposes an interpretable structure on the model. This imposition helps us in the smooth generation of periodic signals that can aid in data augmentation tasks. We ...
زیستشناسی کاربردی, 2018
This investigation was carried out because of the importance of IAA production by bacteria and it... more This investigation was carried out because of the importance of IAA production by bacteria and its role in rooting induction in drought, saline and saline-sodic soils., especially.In this investigation, 400 bacteria were isolated from soils with different levels of electrical conductivity (EC) and sodium adsorption ratio (SAR) and analyzed the variability of their ability in IAA production and the effects of different amounts of tryptophan and drought-salinity tensions on IAA production.Results indicated that the effects of drought and salinity tensions on variability of IAA production were significant, statistically. One of bacterial species as <em>Arthrobacter siccitolerans</em> that was isolated from saline-sodic soils, was IAA producer and had the highest dtability in tensions. Also, this bacterium was osmotolerant and halotolerant and continued to IAA production up to EC of 40 dS.m<sup>-1</sup> and OP (Osmosis pressure) of -20 bar.<em> A. siccitole...
Emergency Radiology, 2020
Introduction CT scan is crucial in evaluating head trauma. However, its inappropriate use will ca... more Introduction CT scan is crucial in evaluating head trauma. However, its inappropriate use will cause unnecessary radiation exposure to patient and financial burden to health systems. Our aim is appraising amount of brain CT scans performed in our Emergency Department (ED) for evaluating mild head trauma which are not indicated according to four standardized guidelines as well as analyzing contributing factors. Methods This was a descriptive prospective study. We included randomly selected adult patients under 75 years old with minor head trauma evaluated by brain CT scan at our ED. For all patients, we completed a checklist including demographic data, mechanism of trauma, specialty of the requesting physician, and whether the patient meets the brain CT guidelines criteria. Brain CT overuse was defined as scans performed for patients without criteria of any of the standardized guidelines. Results We evaluated 170 patients. The mean age of patients was 38.38 ± 19.73 years old. The most common mechanism of trauma was falling (37.6%). The overall brain CT scan overuse was 15.3%. Most of the overused scans were performed in younger patients, and patient's age was inversely correlated to overuse. There was no significant difference based on the mechanism of trauma and the specialty of requesting physician. Discussion Our study accentuates the high frequency of brain CT scan overuse, leading to unnecessary radiation exposure and financial burden on healthcare systems. We emphasize that using a guideline for requesting brain CT scan can eliminate unnecessary scans along with detecting patients with important decisive damages.
Article history Received 4 Oct 2016 Accepted 15 Dec 2016 Available online 15 Mar 2017
Background Ovary is an important reproductive organ for poultry. MicroRNA (miRNA) is a highly con... more Background Ovary is an important reproductive organ for poultry. MicroRNA (miRNA) is a highly conserved class of small non-coding RNA that function in a specific manner to post-transcriptionally regulate gene expression in organisms. Currently, miRNA has been studied extensively but research on duck ovarian tissue is relatively rare. Thus, in this study, we performed the first miRNA analysis of ovarian tissues in Leizhou black ducks with low and high rates of egg production. Method: Using high-throughput sequencing technology, miRNA library was constructed to obtain miRNA expression profile; differentially expressed miRNAs were screened, to predict miRNA target genes. Results A total of 29 differentially expressed miRNAs were obtained from the miRNA library, of which 7 were up-regulated and 22 were down-regulated. Verification of 12 randomly selected miRNAs, using RT-qPCR technology showed the results are consistent with the sequencing data, indicating that the sequencing results ar...
BackgroundEstrogen receptor 2 (ESR2) plays significant biological roles in the reproductive syste... more BackgroundEstrogen receptor 2 (ESR2) plays significant biological roles in the reproductive system and ovarian follicle development. This study, therefore, aimed to reveal the expression pattern and cell-specific localization of ESR2 in the ovarian follicles of Leizhou black ducks. MethodFour laying Leizhou black ducks at 43 weeks old were annihilated and different grade-sized follicles were collected for immunohistochemistry and expression profile study. The follicles were grouped into seven (7) as small white follicles (SWF), large white follicles (LWF), small yellow follicles (SYF), large yellow follicles (LYF), follicle 5 (F5), follicle 2 (F2), and follicle 1 (F1). ResultsThe qRT/PCR results displayed that ESR2 mRNA was expressed in all follicles with the highest (P < 0.05) level of expression found in F1 compared to other follicles. Immunohistochemistry analysis of the cell-specific localization of ESR2 protein revealed that ESR2 was distributed in both granulosa and theca c...
Journal of Medical Virology, 2020
Service: Conceived the project (methodology, validation protocol, samples collections and ethical... more Service: Conceived the project (methodology, validation protocol, samples collections and ethical committee approval) reviewed all patient's medical records, collected the data, wrote the manuscript in collaboration with Benoît Kabamba-Mukadi, reviewed the literature and responded to the reviewers.
Journal of Medical Virology, 2021
The current reliable recommended test for coronavirus disease 2019 (COVID-19) diagnosis is quanti... more The current reliable recommended test for coronavirus disease 2019 (COVID-19) diagnosis is quantitative reverse-transcription polymerase chain reaction (RT-qPCR). Rapid antigen test devices could be useful as they are less expensive, faster without the need of specialized laboratories to perform the test.
Background Carbapenem-resistant Acinetobacter baumannii is considered a top priority pathogen by ... more Background Carbapenem-resistant Acinetobacter baumannii is considered a top priority pathogen by the World Health Organization for combatting increasing antibiotic resistance and development of new drugs. Since it was originally reported in Klebsiella pneumoniae in 2009, the quick spread of the blaNDM−1 gene encoding a New-Delhi metallo-beta-lactamase-1 (NDM-1) is increasingly recognized as a serious threat. This gene is usually carried by large plasmids and has already been documented in diverse bacterial species, including A. baumannii. Here, we report the first detection of a NDM-1-producing A. baumannii strain isolated in Benin. Case presentation: A 31-year-old woman was admitted to a surgical unit with a diagnosis of post-cesarean hematoma. An extensively-drug resistant A. baumannii strain solely susceptible to amikacin, colistin and ciprofloxacin, and resistant to several other antibiotics including ceftazidime, imipenem, meropenem, gentamicin, tobramycin, ceftazidime/avibacta...
Journal of Pain Research, 2020
Purpose: Few papers have studied the objective effects of PRP on cartilage. In this study, we inv... more Purpose: Few papers have studied the objective effects of PRP on cartilage. In this study, we investigated the effect of PRP on cartilage characteristics by special MRI sequencing in knee osteoarthritis (IRCT registration number: 2014020413442N6). Patients and methods: In this double blind randomized clinical trial, patients with bilateral knees osteoarthritis-grade 1, 2, and 3 were included in the study. Each patient's knees were randomly allocated to either control or treatment groups. PRP was injected in two sessions with 4 week intervals in PRP group. The VAS (visual analog scale) and WOMAC (Western Ontario and McMaster Universities Arthritis Index) were utilized and MRI was performed for all patients, before, and 8 months after treatment. The MRI sequences taken were transverse 3D TRUFISP and coronal and sagittal fat saturated proton-density. Imaging was scored according to four cartilage characteristics. Results: 46 knees (from 23 patients) were included in this study. 23 knees in the case group and 23 knees in control group were studied. All patients were female with mean age of 57.57±5.9 years. Mean total WOMAC and VAS changes before and after treatment in control group were 11.61±8.5 and 1.3±1.1 respectively. In PRP group, mean total WOMAC and VAS changes showed better improvement with 20±12.3 and 3.2±1.6 respectively (P-value <0.05). In PRP group, all of the radiologic variables (patellofemoral cartilage volume, synovitis and medial and lateral meniscal disintegrity), with the exception of subarticular bone marrow abnormality, had significant improvement (P-value <0.05). In a comparison between the two groups, patellofemoral cartilage volume and synovitis had significantly changed in the PRP group (P-value <0.05). Conclusion: In this study, in addition to the effect of PRP on VAS and WOMAC, there was a significant effect on radiologic characteristics (patellofemoral cartilage volume and synovitis). For further evaluation, a longer study with a larger sample size is recommended.
Annals of Physical and Rehabilitation Medicine, 2018
Jundishapur Journal of Microbiology, 2016
Background: Cervical cancer is one of the leading causes of cancer-related death in females. Huma... more Background: Cervical cancer is one of the leading causes of cancer-related death in females. Human papilloma virus (HPV) is the major risk factor of cervical cancer. Objectives: The aim of the current study was to explore the frequency and role of 23 different HPVs in patients with cervical cancer. Materials and Methods: Overall, 117 formalin-fix and paraffin-embedded (FFPE) tissues from cervical cancer patients with squamous cell carcinoma (SCC) or dysplasia were collected from Mirza-Kochakkhan-Jangali hospital, Tehran, Iran during year 2013, to investigate the
Petroleum, 2015
Greater complexity is involved in the transient pressure analysis of horizontal oil wells in cont... more Greater complexity is involved in the transient pressure analysis of horizontal oil wells in contrast to vertical wells, as the horizontal wells are considered entirely horizontal and parallel with the top and underneath boundaries of the oil reserve. Therefore, there is an essential need to estimate productivity of horizontal wells accurately to examine the effectiveness of a horizontal well in terms of technical and economic prospects. In this work, novel and rigorous methods based on two different types of intelligent approaches including the artificial neural network (ANN) linked to the particle swarm optimization (PSO) tool are developed to precisely forecast the productivity of horizontal wells under pseudo-steady-state conditions. It was found that there is very good match between the modeling output and the real data taken from the literature, so that a very low average absolute error percentage is attained (e.g., <0.82%). The developed techniques can be also incorporated in the numerical reservoir simulation packages for the purpose of accuracy improvement as well as better parametric sensitivity analysis.
Aging, 2011
The mechanism of chemotherapy-induced acceleration of ovarian aging is not fully understood. We u... more The mechanism of chemotherapy-induced acceleration of ovarian aging is not fully understood. We used doxorubicin, a widely used cancer chemotherapeutic, in a variety of in vivo xenograft, and in vitro models to investigate the impact of chemotherapy-induced aging on the human ovary. Doxorubicin caused massive double-strand-DNA-breaks in primordial follicles, oocytes, and granulosa cells in a dose dependent fashion as revealed by accumulating γH2AX foci. This damage was associated with apoptotic oocyte death and resulted in the activation of ATM. It appeared that the repair response enabled a minor proportion of oocytes (34.7%) and granulosa cells (12.1%) to survive while the majority succumbed to apoptotic death. Paradoxically, inhibition of ATM by KU-55933 resulted in improved survival, probably via prevention of downstream activation of TAp63α. Furthermore, doxorubicin caused vascular and stromal damage in the human ovary, which might impair ovarian function both pre- and post-men...
The Journal of Supercritical Fluids, 2014
Reproductive BioMedicine Online, 2008
Elke Heytens has obtained an MSc degree in biochemistry and a Master's degree in molecular medica... more Elke Heytens has obtained an MSc degree in biochemistry and a Master's degree in molecular medical biotechnology at Ghent University, Belgium. She joined the research team of the Centre for Reproductive Medicine at Ghent University Hospital in 2005. She is currently working on her PhD and her major interest is oocyte activation in assisted reproduction.
Reproductive BioMedicine Online, 2010
Dr Elke Heytens has obtained an MSc degree in biochemistry and a Master's degree in molecular med... more Dr Elke Heytens has obtained an MSc degree in biochemistry and a Master's degree in molecular medical biotechnology at Ghent University, Belgium. She joined the research team of the Centre for Reproductive Medicine at Ghent University Hospital in 2005. During her PhD research she was working on oocyte activation in assisted reproduction, and more particularly in case of male infertility.
PLoS ONE, 2011
Ovarian transplantation is one of the key approaches to restoring fertility in women who became m... more Ovarian transplantation is one of the key approaches to restoring fertility in women who became menopausal as a result of cancer treatments. A major limitation of human ovarian transplants is massive follicular loss during revascularization. Here we investigated whether sphingosine-1-phosphate or its receptor agonists could enhance neoangiogenesis and follicle survival in ovarian transplants in a xenograft model. Human ovarian tissue xenografts in severe-combined-immunodeficient mice were treated with sphingosine-1-phosphate, its analogs, or vehicle for 1-10 days. We found that sphingosine-1phosphate treatment increased vascular density in ovarian transplants significantly whereas FTY720 and SEW2871 had the opposite effect. In addition, sphingosine-1-phosphate accelerated the angiogenic process compared to vehicle-treated controls. Furthermore, sphingosine-1-phosphate treatment was associated with a significant proliferation of ovarian stromal cell as well as reduced necrosis and tissue hypoxia compared to the vehicle-treated controls. This resulted in a significantly lower percentage of apoptotic follicles in sphingosine-1-phosphate-treated transplants. We conclude that while sphingosine-1-phosphate promotes neoangiogenesis in ovarian transplants and reduces ischemic reperfusion injury, sphingosine-1-phosphate receptor agonists appear to functionally antagonize this process. Sphingosine-1-phosphate holds great promise to clinically enhance the survival and longevity of human autologous ovarian transplants.
Medical & Biological Engineering & Computing, 2004
The heart rate is a non-stationary signal, and its variation can contain indicators of current di... more The heart rate is a non-stationary signal, and its variation can contain indicators of current disease or warnings about impending cardiac diseases. The indicators can be present at all times or can occur at random, during certain intervals of the day. However, to study and pinpoint abnormalities in large quantities of data collected over several hours is strenuous and time consuming. Hence, heart rate variation measurement (instantaneous heart rate against time) has become a popular, non-invasive tool for assessing the autonomic nervous system. Computerbased analytical tools for the in-depth study and classification of data over day-long intervals can be very useful in diagnostics. The paper deals with the classification of cardiac rhythms using an artificial neural network and fuzzy relationships. The results indicate a high level of efficacy of the tools used, with an accuracy level of 80-85%
Information
Physiological and kinematic signals from humans are often used for monitoring health. Several pro... more Physiological and kinematic signals from humans are often used for monitoring health. Several processes of interest (e.g., cardiac and respiratory processes, and locomotion) demonstrate periodicity. Training models for inference on these signals (e.g., detection of anomalies, and extraction of biomarkers) require large amounts of data to capture their variability, which are not readily available. This hinders the performance of complex inference models. In this work, we introduce a methodology for improving inference on such signals by incorporating phase-based interpretability and other inference tasks into a multi-task framework applied to a generative model. For this purpose, we utilize phase information as a regularization term and as an input to the model and introduce an interpretable unit in a neural network, which imposes an interpretable structure on the model. This imposition helps us in the smooth generation of periodic signals that can aid in data augmentation tasks. We ...
زیستشناسی کاربردی, 2018
This investigation was carried out because of the importance of IAA production by bacteria and it... more This investigation was carried out because of the importance of IAA production by bacteria and its role in rooting induction in drought, saline and saline-sodic soils., especially.In this investigation, 400 bacteria were isolated from soils with different levels of electrical conductivity (EC) and sodium adsorption ratio (SAR) and analyzed the variability of their ability in IAA production and the effects of different amounts of tryptophan and drought-salinity tensions on IAA production.Results indicated that the effects of drought and salinity tensions on variability of IAA production were significant, statistically. One of bacterial species as <em>Arthrobacter siccitolerans</em> that was isolated from saline-sodic soils, was IAA producer and had the highest dtability in tensions. Also, this bacterium was osmotolerant and halotolerant and continued to IAA production up to EC of 40 dS.m<sup>-1</sup> and OP (Osmosis pressure) of -20 bar.<em> A. siccitole...
Emergency Radiology, 2020
Introduction CT scan is crucial in evaluating head trauma. However, its inappropriate use will ca... more Introduction CT scan is crucial in evaluating head trauma. However, its inappropriate use will cause unnecessary radiation exposure to patient and financial burden to health systems. Our aim is appraising amount of brain CT scans performed in our Emergency Department (ED) for evaluating mild head trauma which are not indicated according to four standardized guidelines as well as analyzing contributing factors. Methods This was a descriptive prospective study. We included randomly selected adult patients under 75 years old with minor head trauma evaluated by brain CT scan at our ED. For all patients, we completed a checklist including demographic data, mechanism of trauma, specialty of the requesting physician, and whether the patient meets the brain CT guidelines criteria. Brain CT overuse was defined as scans performed for patients without criteria of any of the standardized guidelines. Results We evaluated 170 patients. The mean age of patients was 38.38 ± 19.73 years old. The most common mechanism of trauma was falling (37.6%). The overall brain CT scan overuse was 15.3%. Most of the overused scans were performed in younger patients, and patient's age was inversely correlated to overuse. There was no significant difference based on the mechanism of trauma and the specialty of requesting physician. Discussion Our study accentuates the high frequency of brain CT scan overuse, leading to unnecessary radiation exposure and financial burden on healthcare systems. We emphasize that using a guideline for requesting brain CT scan can eliminate unnecessary scans along with detecting patients with important decisive damages.
Article history Received 4 Oct 2016 Accepted 15 Dec 2016 Available online 15 Mar 2017
Background Ovary is an important reproductive organ for poultry. MicroRNA (miRNA) is a highly con... more Background Ovary is an important reproductive organ for poultry. MicroRNA (miRNA) is a highly conserved class of small non-coding RNA that function in a specific manner to post-transcriptionally regulate gene expression in organisms. Currently, miRNA has been studied extensively but research on duck ovarian tissue is relatively rare. Thus, in this study, we performed the first miRNA analysis of ovarian tissues in Leizhou black ducks with low and high rates of egg production. Method: Using high-throughput sequencing technology, miRNA library was constructed to obtain miRNA expression profile; differentially expressed miRNAs were screened, to predict miRNA target genes. Results A total of 29 differentially expressed miRNAs were obtained from the miRNA library, of which 7 were up-regulated and 22 were down-regulated. Verification of 12 randomly selected miRNAs, using RT-qPCR technology showed the results are consistent with the sequencing data, indicating that the sequencing results ar...
BackgroundEstrogen receptor 2 (ESR2) plays significant biological roles in the reproductive syste... more BackgroundEstrogen receptor 2 (ESR2) plays significant biological roles in the reproductive system and ovarian follicle development. This study, therefore, aimed to reveal the expression pattern and cell-specific localization of ESR2 in the ovarian follicles of Leizhou black ducks. MethodFour laying Leizhou black ducks at 43 weeks old were annihilated and different grade-sized follicles were collected for immunohistochemistry and expression profile study. The follicles were grouped into seven (7) as small white follicles (SWF), large white follicles (LWF), small yellow follicles (SYF), large yellow follicles (LYF), follicle 5 (F5), follicle 2 (F2), and follicle 1 (F1). ResultsThe qRT/PCR results displayed that ESR2 mRNA was expressed in all follicles with the highest (P < 0.05) level of expression found in F1 compared to other follicles. Immunohistochemistry analysis of the cell-specific localization of ESR2 protein revealed that ESR2 was distributed in both granulosa and theca c...
Journal of Medical Virology, 2020
Service: Conceived the project (methodology, validation protocol, samples collections and ethical... more Service: Conceived the project (methodology, validation protocol, samples collections and ethical committee approval) reviewed all patient's medical records, collected the data, wrote the manuscript in collaboration with Benoît Kabamba-Mukadi, reviewed the literature and responded to the reviewers.
Journal of Medical Virology, 2021
The current reliable recommended test for coronavirus disease 2019 (COVID-19) diagnosis is quanti... more The current reliable recommended test for coronavirus disease 2019 (COVID-19) diagnosis is quantitative reverse-transcription polymerase chain reaction (RT-qPCR). Rapid antigen test devices could be useful as they are less expensive, faster without the need of specialized laboratories to perform the test.
Background Carbapenem-resistant Acinetobacter baumannii is considered a top priority pathogen by ... more Background Carbapenem-resistant Acinetobacter baumannii is considered a top priority pathogen by the World Health Organization for combatting increasing antibiotic resistance and development of new drugs. Since it was originally reported in Klebsiella pneumoniae in 2009, the quick spread of the blaNDM−1 gene encoding a New-Delhi metallo-beta-lactamase-1 (NDM-1) is increasingly recognized as a serious threat. This gene is usually carried by large plasmids and has already been documented in diverse bacterial species, including A. baumannii. Here, we report the first detection of a NDM-1-producing A. baumannii strain isolated in Benin. Case presentation: A 31-year-old woman was admitted to a surgical unit with a diagnosis of post-cesarean hematoma. An extensively-drug resistant A. baumannii strain solely susceptible to amikacin, colistin and ciprofloxacin, and resistant to several other antibiotics including ceftazidime, imipenem, meropenem, gentamicin, tobramycin, ceftazidime/avibacta...
Journal of Pain Research, 2020
Purpose: Few papers have studied the objective effects of PRP on cartilage. In this study, we inv... more Purpose: Few papers have studied the objective effects of PRP on cartilage. In this study, we investigated the effect of PRP on cartilage characteristics by special MRI sequencing in knee osteoarthritis (IRCT registration number: 2014020413442N6). Patients and methods: In this double blind randomized clinical trial, patients with bilateral knees osteoarthritis-grade 1, 2, and 3 were included in the study. Each patient's knees were randomly allocated to either control or treatment groups. PRP was injected in two sessions with 4 week intervals in PRP group. The VAS (visual analog scale) and WOMAC (Western Ontario and McMaster Universities Arthritis Index) were utilized and MRI was performed for all patients, before, and 8 months after treatment. The MRI sequences taken were transverse 3D TRUFISP and coronal and sagittal fat saturated proton-density. Imaging was scored according to four cartilage characteristics. Results: 46 knees (from 23 patients) were included in this study. 23 knees in the case group and 23 knees in control group were studied. All patients were female with mean age of 57.57±5.9 years. Mean total WOMAC and VAS changes before and after treatment in control group were 11.61±8.5 and 1.3±1.1 respectively. In PRP group, mean total WOMAC and VAS changes showed better improvement with 20±12.3 and 3.2±1.6 respectively (P-value <0.05). In PRP group, all of the radiologic variables (patellofemoral cartilage volume, synovitis and medial and lateral meniscal disintegrity), with the exception of subarticular bone marrow abnormality, had significant improvement (P-value <0.05). In a comparison between the two groups, patellofemoral cartilage volume and synovitis had significantly changed in the PRP group (P-value <0.05). Conclusion: In this study, in addition to the effect of PRP on VAS and WOMAC, there was a significant effect on radiologic characteristics (patellofemoral cartilage volume and synovitis). For further evaluation, a longer study with a larger sample size is recommended.
Annals of Physical and Rehabilitation Medicine, 2018
Jundishapur Journal of Microbiology, 2016
Background: Cervical cancer is one of the leading causes of cancer-related death in females. Huma... more Background: Cervical cancer is one of the leading causes of cancer-related death in females. Human papilloma virus (HPV) is the major risk factor of cervical cancer. Objectives: The aim of the current study was to explore the frequency and role of 23 different HPVs in patients with cervical cancer. Materials and Methods: Overall, 117 formalin-fix and paraffin-embedded (FFPE) tissues from cervical cancer patients with squamous cell carcinoma (SCC) or dysplasia were collected from Mirza-Kochakkhan-Jangali hospital, Tehran, Iran during year 2013, to investigate the
Petroleum, 2015
Greater complexity is involved in the transient pressure analysis of horizontal oil wells in cont... more Greater complexity is involved in the transient pressure analysis of horizontal oil wells in contrast to vertical wells, as the horizontal wells are considered entirely horizontal and parallel with the top and underneath boundaries of the oil reserve. Therefore, there is an essential need to estimate productivity of horizontal wells accurately to examine the effectiveness of a horizontal well in terms of technical and economic prospects. In this work, novel and rigorous methods based on two different types of intelligent approaches including the artificial neural network (ANN) linked to the particle swarm optimization (PSO) tool are developed to precisely forecast the productivity of horizontal wells under pseudo-steady-state conditions. It was found that there is very good match between the modeling output and the real data taken from the literature, so that a very low average absolute error percentage is attained (e.g., <0.82%). The developed techniques can be also incorporated in the numerical reservoir simulation packages for the purpose of accuracy improvement as well as better parametric sensitivity analysis.
Aging, 2011
The mechanism of chemotherapy-induced acceleration of ovarian aging is not fully understood. We u... more The mechanism of chemotherapy-induced acceleration of ovarian aging is not fully understood. We used doxorubicin, a widely used cancer chemotherapeutic, in a variety of in vivo xenograft, and in vitro models to investigate the impact of chemotherapy-induced aging on the human ovary. Doxorubicin caused massive double-strand-DNA-breaks in primordial follicles, oocytes, and granulosa cells in a dose dependent fashion as revealed by accumulating γH2AX foci. This damage was associated with apoptotic oocyte death and resulted in the activation of ATM. It appeared that the repair response enabled a minor proportion of oocytes (34.7%) and granulosa cells (12.1%) to survive while the majority succumbed to apoptotic death. Paradoxically, inhibition of ATM by KU-55933 resulted in improved survival, probably via prevention of downstream activation of TAp63α. Furthermore, doxorubicin caused vascular and stromal damage in the human ovary, which might impair ovarian function both pre- and post-men...
The Journal of Supercritical Fluids, 2014
Reproductive BioMedicine Online, 2008
Elke Heytens has obtained an MSc degree in biochemistry and a Master's degree in molecular medica... more Elke Heytens has obtained an MSc degree in biochemistry and a Master's degree in molecular medical biotechnology at Ghent University, Belgium. She joined the research team of the Centre for Reproductive Medicine at Ghent University Hospital in 2005. She is currently working on her PhD and her major interest is oocyte activation in assisted reproduction.
Reproductive BioMedicine Online, 2010
Dr Elke Heytens has obtained an MSc degree in biochemistry and a Master's degree in molecular med... more Dr Elke Heytens has obtained an MSc degree in biochemistry and a Master's degree in molecular medical biotechnology at Ghent University, Belgium. She joined the research team of the Centre for Reproductive Medicine at Ghent University Hospital in 2005. During her PhD research she was working on oocyte activation in assisted reproduction, and more particularly in case of male infertility.
PLoS ONE, 2011
Ovarian transplantation is one of the key approaches to restoring fertility in women who became m... more Ovarian transplantation is one of the key approaches to restoring fertility in women who became menopausal as a result of cancer treatments. A major limitation of human ovarian transplants is massive follicular loss during revascularization. Here we investigated whether sphingosine-1-phosphate or its receptor agonists could enhance neoangiogenesis and follicle survival in ovarian transplants in a xenograft model. Human ovarian tissue xenografts in severe-combined-immunodeficient mice were treated with sphingosine-1-phosphate, its analogs, or vehicle for 1-10 days. We found that sphingosine-1phosphate treatment increased vascular density in ovarian transplants significantly whereas FTY720 and SEW2871 had the opposite effect. In addition, sphingosine-1-phosphate accelerated the angiogenic process compared to vehicle-treated controls. Furthermore, sphingosine-1-phosphate treatment was associated with a significant proliferation of ovarian stromal cell as well as reduced necrosis and tissue hypoxia compared to the vehicle-treated controls. This resulted in a significantly lower percentage of apoptotic follicles in sphingosine-1-phosphate-treated transplants. We conclude that while sphingosine-1-phosphate promotes neoangiogenesis in ovarian transplants and reduces ischemic reperfusion injury, sphingosine-1-phosphate receptor agonists appear to functionally antagonize this process. Sphingosine-1-phosphate holds great promise to clinically enhance the survival and longevity of human autologous ovarian transplants.
Medical & Biological Engineering & Computing, 2004
The heart rate is a non-stationary signal, and its variation can contain indicators of current di... more The heart rate is a non-stationary signal, and its variation can contain indicators of current disease or warnings about impending cardiac diseases. The indicators can be present at all times or can occur at random, during certain intervals of the day. However, to study and pinpoint abnormalities in large quantities of data collected over several hours is strenuous and time consuming. Hence, heart rate variation measurement (instantaneous heart rate against time) has become a popular, non-invasive tool for assessing the autonomic nervous system. Computerbased analytical tools for the in-depth study and classification of data over day-long intervals can be very useful in diagnostics. The paper deals with the classification of cardiac rhythms using an artificial neural network and fuzzy relationships. The results indicate a high level of efficacy of the tools used, with an accuracy level of 80-85%