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Ethiopian Journal of Health Sciences
BACKGROUND፡ Accurate diagnosis of Giardia lamblia and Entamoeba histolytica is important since th... more BACKGROUND፡ Accurate diagnosis of Giardia lamblia and Entamoeba histolytica is important since these intestinal parasites account for a significant proportion of morbidity and mortality globally. Microscopy is the key diagnostic test used for diagnosis of the two parasites. Other tests including rapid diagnostic tests and polymerase chain reaction have been developed to improve the detection of these parasites. Most of these newer tests are not affordable in resource limited settings, hence the over reliance on microscopy. The objective of this study was to determine the reliability of microscopy in a resource limited setting in Western Kenya, a region endemic for the two intestinal parasites.METHODS: Polymerase chain reaction, the gold standard test, was performed on stool samples suspected for G. lamblia and E. histolytica. Microscopy was then performed on the same samples and the two tests compared.RESULTS: Microscopy was found to be 64.4% sensitive, 86.6% specific for the detect...
Merit Research Journal of Medicine and Medical Sciences
According 2013, with 82% of these cases occurring in Africa. 584,000 malaria deaths worldwide. pa... more According 2013, with 82% of these cases occurring in Africa. 584,000 malaria deaths worldwide. parasites is determined by other immune system When combined in therapy, immunostimulatory oligodeoxynucleotides (ODNs) parasitic mechanisms providing effective protection in ODN cell B-cells, functionality, the cytokine expression of physiologic factors and this can influence disease severity physiologic biomolecules like m (ANGPTs) cytokines and CpG ODNs during malaria. ODN co BALB/c mice groups infe parasites five consecutive days included for comparison parasitaemia development mice were humanely for measuring various physiologic factors gene in adiponectin, ANGPT1, delevations in ANGPT2, MMP largely agreeable with data from combinatorial preventative and therapeutic anti Key Physiologic Biomolecules.
Malaria is a leading cause of morbidity and mortality especially among children, expectant women ... more Malaria is a leading cause of morbidity and mortality especially among children, expectant women and continues to be a global health burden. Haematological changes mark some of the most common complications in malaria as they play a major role in malaria pathology. Thrombocytes in particular, have been shown to bind infected erythrocytes and kill intracellular malaria parasites thereby indicating a protective function of platelets in the early stages. It is however not clear the mechanisms that leads to low thrombocytes count in malaria infected individuals. In trying to understand the mechanisms leading to low thrombocyte count in malaria infected individuals, this study sort to establish the association between platelet count and P. falciparum infection amongst children less than five years. This was a retrospective case-control study, n =549. Children below the age of five years that attending Kombewa Sub County Hospital were recruited. Study participants were identified using th...
Background Malaria remains a leading cause of morbidity and mortality in Kenya, especially in you... more Background Malaria remains a leading cause of morbidity and mortality in Kenya, especially in young children and pregnant women. Due to widespread resistance of Plasmodium falciparum to drugs such as Chloroquine (CQ) and Sulfurdoxine-Pyrthamine (SP), Artemisisnin Combination Therapy (ACT) was adopted in Africa as a means of improving treatment efficacy and slowing the spread of resistance. The development of drug resistance by the parasites for the various malaria drug regimens that have been in use before has been attributed to point mutations within the parasite genome. Therefore this study investigated the prevalence of point mutations in selected codons of the pfcrt and pfmdr-1 genes of Plasmodium falciparum. It is however unclear whether ACT will be effective in preventing the selection of resistant parasites in Africa, where parasite transmission rates are generally much higher with parts of Asia and Africa already reporting a reduction in sensitivity to ACT. Methods The dot-b...
Ethiopian Journal of Health Sciences
BACKGROUND፡ Accurate diagnosis of Giardia lamblia and Entamoeba histolytica is important since th... more BACKGROUND፡ Accurate diagnosis of Giardia lamblia and Entamoeba histolytica is important since these intestinal parasites account for a significant proportion of morbidity and mortality globally. Microscopy is the key diagnostic test used for diagnosis of the two parasites. Other tests including rapid diagnostic tests and polymerase chain reaction have been developed to improve the detection of these parasites. Most of these newer tests are not affordable in resource limited settings, hence the over reliance on microscopy. The objective of this study was to determine the reliability of microscopy in a resource limited setting in Western Kenya, a region endemic for the two intestinal parasites.METHODS: Polymerase chain reaction, the gold standard test, was performed on stool samples suspected for G. lamblia and E. histolytica. Microscopy was then performed on the same samples and the two tests compared.RESULTS: Microscopy was found to be 64.4% sensitive, 86.6% specific for the detect...
Merit Research Journal of Medicine and Medical Sciences
According 2013, with 82% of these cases occurring in Africa. 584,000 malaria deaths worldwide. pa... more According 2013, with 82% of these cases occurring in Africa. 584,000 malaria deaths worldwide. parasites is determined by other immune system When combined in therapy, immunostimulatory oligodeoxynucleotides (ODNs) parasitic mechanisms providing effective protection in ODN cell B-cells, functionality, the cytokine expression of physiologic factors and this can influence disease severity physiologic biomolecules like m (ANGPTs) cytokines and CpG ODNs during malaria. ODN co BALB/c mice groups infe parasites five consecutive days included for comparison parasitaemia development mice were humanely for measuring various physiologic factors gene in adiponectin, ANGPT1, delevations in ANGPT2, MMP largely agreeable with data from combinatorial preventative and therapeutic anti Key Physiologic Biomolecules.
Malaria is a leading cause of morbidity and mortality especially among children, expectant women ... more Malaria is a leading cause of morbidity and mortality especially among children, expectant women and continues to be a global health burden. Haematological changes mark some of the most common complications in malaria as they play a major role in malaria pathology. Thrombocytes in particular, have been shown to bind infected erythrocytes and kill intracellular malaria parasites thereby indicating a protective function of platelets in the early stages. It is however not clear the mechanisms that leads to low thrombocytes count in malaria infected individuals. In trying to understand the mechanisms leading to low thrombocyte count in malaria infected individuals, this study sort to establish the association between platelet count and P. falciparum infection amongst children less than five years. This was a retrospective case-control study, n =549. Children below the age of five years that attending Kombewa Sub County Hospital were recruited. Study participants were identified using th...
Background Malaria remains a leading cause of morbidity and mortality in Kenya, especially in you... more Background Malaria remains a leading cause of morbidity and mortality in Kenya, especially in young children and pregnant women. Due to widespread resistance of Plasmodium falciparum to drugs such as Chloroquine (CQ) and Sulfurdoxine-Pyrthamine (SP), Artemisisnin Combination Therapy (ACT) was adopted in Africa as a means of improving treatment efficacy and slowing the spread of resistance. The development of drug resistance by the parasites for the various malaria drug regimens that have been in use before has been attributed to point mutations within the parasite genome. Therefore this study investigated the prevalence of point mutations in selected codons of the pfcrt and pfmdr-1 genes of Plasmodium falciparum. It is however unclear whether ACT will be effective in preventing the selection of resistant parasites in Africa, where parasite transmission rates are generally much higher with parts of Asia and Africa already reporting a reduction in sensitivity to ACT. Methods The dot-b...