rofiq sunaryanto - Academia.edu (original) (raw)

Papers by rofiq sunaryanto

Research paper thumbnail of Perbandingan Emisi Gas Buang Antara Motor Bahan Bakar Empat Tak Berbahan Bakar Premium, Pertalite, Dan Pertamax

JURNAL TECHLINK, Oct 1, 2023

Research paper thumbnail of Phytotoxic and Cytotoxic Effects, Antioxidant Potentials, and Phytochemical Constituents of <i>Stevia rebaudiana</i> Leaves

Research paper thumbnail of Isolation and screening of actinomycetes active against plant pathogenic fungi

AIP conference proceedings, 2024

Research paper thumbnail of Fermentation medium optimization of Streptomyces sp. as an antifungal agent against the Ganoderma boninensis pathogen in oil palm

Indonesian Journal of Biotechnology, Dec 29, 2023

Ganoderma boninensis is the most common fungus which attacks oil palm trees. However, a significa... more Ganoderma boninensis is the most common fungus which attacks oil palm trees. However, a significant percentage of inhibition to the problem is found through the use of Streptomyces sp. The optimization of the Streptomyces sp. fermentation medium growth factors affects the secondary metabolites production. This study aimed to identify the best formulation of carbon and nitrogen sources and the optimum concentration of Streptomyces sp. fermentation medium for antifungal compound production. The results showed that the best sources of carbon and nitrogen were liquid glucose and monosodium glutamate in the inhibition zones of 16.7 mm and 6.3 mm, while the best concentration levels were 20 g/L and 14.19 g/L, respectively. The results of the first optimization showed an inhibition zone response and area (%) of the optimum high-performance liquid chromatography (HPLC) chromatogram of 24.39 mm and 62.68 percent, respectively. Taking the suggestion of the first optimization, the second optimization produced 15.2 g/L and 8.3 g/L. The predicted value of the inhibition zone was 21.47 mm, and the area (%) of the HPLC chromatogram was 53.44 percent. The validation results showed an inhibition zone response of 22.01 mm and an HPLC chromatogram area (%) of 54.86 percent. The difference between the predicted and validation values was less than 5 percent; the validation value was thus in line with the value predicted by Design Expert 10.0.7. The chemical formula of the probable active compound is that of the cyclo(phenylalanyl-prolyl) compound.

Research paper thumbnail of Streptomyces costaricanus RS25 with bioactivity against plant pathogenic fungus

AIP Conference Proceedings, Dec 31, 2022

Research paper thumbnail of Isolation and Characterization of Thraustochytrium Trk-23 Producing Docosahexaenoic Acid from North Kalimantan, Indonesia

Pakistan Journal of Biological Sciences, Oct 14, 2023

Research paper thumbnail of Pengaruh Emisi Cerobong Boiler Batubara Terhadap Udara Ambien

JURNAL TECHLINK, Sep 24, 2023

Research paper thumbnail of Oxygen Depletion in a Faroese Sill Fjord

Research paper thumbnail of Chemometric analysis based on GC-MS chemical profiles of essential oil and extracts of black cumin (Nigella sativa L.) and their antioxidant potentials

journal of applied pharmaceutical science, Dec 31, 2022

Research paper thumbnail of EFEKTIVITAS CANGKANG BUAH PALA (Myristica fragrans) SEBAGAI KARBON AKTIF DALAM PENGOLAHAN AIR LIMBAH DOMESTIK

Activated carbon is a material that contains high carbon elements and has a porous solid. The mat... more Activated carbon is a material that contains high carbon elements and has a porous solid. The material used in this research is a nutmeg shell. The purpose of this study was to determine the effectiveness of activated charcoal from the shell of nutmeg (Myristica fragrans) as activated carbon in treating domestic wastewater. The variables studied to compare the effect of the wastewater absorption process are the concentration of the chemical NaOH 15% with temperature variations of 400ºC, 450ºC, and 500ºC. The best activation results were obtained at a temperature of 450ºC with a water content of 4.22%, an ash content of 10.24%, and iodine content of 577,395 mg/g. Activated charcoal parameters refer to SNI 06-3730-1995 regarding the quality standard requirements for activated charcoal. Wastewater management by activated charcoal from the shell of nutmeg (Myristica fragrans) with a concentration of 10, 15, 20 grams of activated charcoal. The best results were obtained by the concentration of activated charcoal 20 grams with a pH value of 9.0, TSS 0.663 mg/L, BOD 7.3 mg/L. For pH parameters, the effectiveness of activated charcoal is less than the maximum, and it does not meet quality standards.

Research paper thumbnail of Kemampuan Mikroba Konsorsium Untuk Aplikasi Pengolahan Limbah Tekstil

PROSIDING, Jul 19, 2022

Industri tekstil merupakan industri penyumbang devisa negara nomor tiga setelah pariwisata dan ke... more Industri tekstil merupakan industri penyumbang devisa negara nomor tiga setelah pariwisata dan kelapa sawit. Namun demikian industri tekstil di Indonesia dihadapkan pada permasalahan lingkungan dimana industri ini dikenal menghasilkan limbah yang cukup besar. Disamping menghasilkan zat pewarna, industri tekstil juga menghasilkan limbah cair dengan pH basa, mengandung fenol, logam berat, surfaktan dan beberapa jenis larutan penghilang kanji. Industri tekstil biasanya menghasilkan limbah dengan BOD yang cukup tinggi dan sebagian besar adalah limbah organik. Pengolahan limbah tekstil yang kaya akan limbah organik dengan BOD yang tinggi lebih tepat digunakan pengolahan limbah secara biologi dengan memanfaatkan mikroba konsorsium. Telah dilakukan uji kemampuan mikroba konsorsium yang terdiri dari Bacillus sp, Bacillus thuringiensis, Bacillus subtilis, dan Pseudomonas sp untuk pengolahan limbah tekstil. Penggunaan mikroba konsorsium ini mampu menurunkan kadar BOD dari 875 mg/L menjadi 95 mg/L, kadar COD 1875 mg/L menjadi 105 mg/L, SV30 menit dari 95% menjadi 85%, SV24 jam dari 67% menjadi 32%. Mikroba konsorsium ini berpotensi untuk dapat digunakan untuk pengolahan limbah dengan sistem lumpur aktif (active sludge).

Research paper thumbnail of Isolation, Identification, and Evaluation of Antimicrobial Activity of Active Compound Produced by Marine Actinomycetes isolate A32

ANNALES BOGORIENSES, Dec 18, 2015

The continuation of new antibiotics exploration becomes an important research program in the worl... more The continuation of new antibiotics exploration becomes an important research program in the world for pharmaceutical and agricultural applications. Marine filamentous bacteria such as actinomycetes have been widely used as an important biological tool to generate a variety of new secondary metabolites, such as antibiotic. The aim of this study was to obtain identified active compound and determine its antimicrobial activity. Isolation, identification, and antimicrobial activity assay of active compound produced by marine actinomycetes isolate A32 had been conducted. Production of active compound using isolate actinomycetes A32 was conducted involving glucose, yeast, peptone medium. The fermentation was carried out at 30 ºC for 5 days. The broth of supernatant was extracted using ethyl acetate. Purification of active compound used chromatography column and eluted stepwise with the chloroform and methanol solvents. Antimicrobial activity was monitored using agar disc diffusion, and microbial test was conducted by analyzing the samples diameter of clear zone towards Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 66923, and Candida albican BIOMCC 00122. Results of isolation and purification of active compound produced by actinomycetes isolate A32 show that this compound has a molecular weight of 503.1 g/mol with molecular formula C 26 H 37 N 3 O 7. Furthermore, this compound was suspected as Madumycin II after analysis of spectrum using 1 HNMR and COSY. The antimicrobial activity assay confirms that this active compound inhibited the growth of Staphylococcus aureus ATCC 25923 and Candida albican BIOMCC 00122.

Research paper thumbnail of PENGARUH WAKTU AERASI TERHADAP LIMBAH KEDELAI MENGGUNAKAN BAKTERI Pseudomonas Putida

Industrial liquid waste in liquid form resulting from tempe production can pollute the environmen... more Industrial liquid waste in liquid form resulting from tempe production can pollute the environment which is detrimental to the surrounding community. According to the World Health Organization (WHO), waste is the result of human activities that are useless, not used or something that is thrown into the environment. Tempe industrial wastewater is very disturbing to waters if it is not managed properly before being discharged into water bodies because this waste causes an unpleasant odor and triggers the growth of various pathogenic bacteria. If the waste is directly disposed of into the waters, in a relatively short time it will cause a foul odor from H2S gas or ammonia as a result of the crying of the organic waste. The existence of a decomposition process will cause an unpleasant odor (Wiryani Erry, 2012). The process of making tempeh in each of its stages uses an average of water for washing, soaking, and boiling the soybeans. This means that quite a lot of liquid waste is produced. This study aims to analyze the content of soybean liquid waste to reduce BOD levels to make it more environmentally friendly. With the aim of reducing BOD levels, this study will use the Pseudomonas Putida bacterium as a waste decomposer, bioreceptors and microelectrodes as practical BOD measuring devices. The sample used is boiled liquid waste from soybean skin in the process of making tempe at one of the tempe entrepreneurs near the Satya Negara University of Indonesia. The sample was then tested to see the content of DO, BOD, temperature, pH, TDS, and total bacteria. The results of research on soybean liquid waste showed that the content of BOD and TDS exceeded the quality standards set by the Regulation of the State Minister for the Environment 03 of 2010 concerning the Environment.

Research paper thumbnail of EFEKTIVITAS CANGKANG BUAH PALA (Myristica fragrans) SEBAGAI KARBON AKTIF DALAM PENGOLAHAN AIR LIMBAH DOMESTIK

JURNAL TECHLINK

Activated carbon is a material that contains high carbon elements and has a porous solid. The mat... more Activated carbon is a material that contains high carbon elements and has a porous solid. The material used in this research is a nutmeg shell. The purpose of this study was to determine the effectiveness of activated charcoal from the shell of nutmeg (Myristica fragrans) as activated carbon in treating domestic wastewater. The variables studied to compare the effect of the wastewater absorption process are the concentration of the chemical NaOH 15% with temperature variations of 400ºC, 450ºC, and 500ºC. The best activation results were obtained at a temperature of 450ºC with a water content of 4.22%, an ash content of 10.24%, and iodinecontent of 577,395 mg/g. Activated charcoal parameters refer to SNI 06-3730-1995 regarding the quality standard requirements for activated charcoal. Wastewater management by activated charcoal from the shell of nutmeg (Myristica fragrans) with a concentration of 10, 15, 20 grams of activated charcoal. The best results were obtained by the concentrati...

Research paper thumbnail of Isolation, Purification, and Characterization of Antimicrobial Substances from Endophytic Actinomycetes

Makara Seri Sains, Feb 28, 2014

Antimicrobial active substances produced by endophytic actinomycetes were isolated and purified. ... more Antimicrobial active substances produced by endophytic actinomycetes were isolated and purified. Plant samples were obtained from four different medicinal plants namely Curcuma domestica, Phaleria macrocarpa, Isotoma longiflora, and Symplocos cocchinensis. Isolation of actinomycetes was conducted using HV agar with the addition of cycloheximide, nystatin, nalidixic acid, and rifamycin. A total of 21 actinomycete isolates were obtained and tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, and Bacillus subtilis ATCC 66923. Among the isolates, isolate KY01 was the most active to Gram-positive and Gram-negative bacteria. Morphological observation and identification using 16S rRNA gene sequence showed that the isolate KY01 was similar to Streptomyces antibioticus. An active compound from the isolate KY01 was produced using yeast peptone medium. The active compound was purified using silica-gel-column chromatography and preparative high performance liquid chromatography (HPLC). A single peak of the active compound was detected with HPLC and LCMS, which showed a retention time of 26.6 min and molecular weight (MW) 906.4474 g/mol, respectively. Abstrak Isolasi, Purifikasi, dan Karakterisasi Senyawa Antimikroba dari Aktinomisetes Endofit. Isolasi dan purifikasi senyawa aktif anti mikroba yang dihasilkan oleh aktinomisetes endofit telah dilakukan. Sampel tanaman diperoleh dari empat jenis tanaman obat yaitu Curcuma domestica, Phaleria macrocarpa, Isotoma longiflora, dan Symplocos cocchinensis. Isolasi aktinomistes endofit dilakukan dengan menggunakan medium HV agar dengan penambahan antibiotik sikloheksimida, nistatin, asam nalidik, dan rifamisin. Sebanyak 21 isolat aktinomisetes diperoleh dari sampel tanaman obat yang selanjutnya diuji aktivitas antimikroba terhadap Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, dan Bacillus subtilis ATCC 66923. Hasil pengujian pada 21 isolat yang diperoleh, isolat KY01 menunjukkan aktivitas antimikroba paling kuat terhadap bakteri Gram-positif maupun bakteri Gram-negatif. Pengamatan secara morfologi dan identifikasi menggunakan sequence gen 16 rRNA menunjukkan bahwa isolat KY01 memiliki kemiripan dengan Streptomyces antibioticus. Produksi senyawa aktif isolat KY01 dengan kultur cair menggunakan medium yeast pepton. Purifikasi senyawa aktif dari ekstrak fermentasi dilakukan menggunakan kromatografi kolom silica gel dan HPLC preparatif. Hasil identifikasi menggunakan HPLC menunjukkan puncak dengan waktu retensi 26,6 menit dan hasil karakterisasi senyawa aktif menggunakan LCMS menunjukkan bobot molekul senyawa aktif sebesar (MW) 906,4474 g/mol.

Research paper thumbnail of Pengembangan Metode Analisis Benzena, Toluena, Dan Xylena Dalam Udara Lingkungan Kerja Menggunakan in House Metode DI PT Unilab Perdana

JURNAL TECHLINK

Benzene, Toluene and Xylene (BTX) compounds are compounds that have colorless and flammable chara... more Benzene, Toluene and Xylene (BTX) compounds are compounds that have colorless and flammable characteristics, have a sweet odor with a low toxicity level, and are not soluble in water. This compound is often used as a solvent, extractor and as a chemical intermediary. The testing of BTX parameters in the working environment refers to the National Institute of Occupational Safety and Health (NIOSH) method number 1501. The method must be modified or developed in terms of equipment and instruments, due to looking at the condition of the materials and equipment in PT UP, i.e. using different columns, capillary columns, fused silica, 30m x 0.32-mm ID; 1-μm film 100% PEG becomes a Rtx-5MS column, and measurements using a FID detector become a Mass Spectrometer detector. And the solvent used is Carbon Disulfiida (CS2) to Methanol for gas chromatography. CS2 solvents are carcinogenic in the body so that they are harmful to human health. Besides being harmful to health, the waste produced by ...

Research paper thumbnail of Cyclo (Tyrosyl-Prolyl) Produced by sp.: Bioactivity and Molecular Structure Elucidation Streptomyces

Pseudomonas aeruginosa Staphylococcus aureus Bacillus subtilis Streptomyces Streptomyces Escheric... more Pseudomonas aeruginosa Staphylococcus aureus Bacillus subtilis Streptomyces Streptomyces Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Bacillus subtilis Streptomyces Determination of bioactivity by minimum inhibitory concentration (MIC) methods and molecular structure identification of antibiotic produced by sp. have been carried out. The antibiotic was produced by liquid culture using sp. isolate. Purification of antibiotic was carried out by silica gel column chromatography and preparative HPLC. Molecular structure identification was carried out using ESI-MS, H NMR, C NMR, and C DEPT NMR. Pure antibiotic showed inhibition activity to Gram-negative and Gram-positive bacteria. MIC to ATCC 25922,

Research paper thumbnail of Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp

Microbiology Indonesia, Aug 1, 2010

A total of sixty eight actinomycetes were isolated from near sea shore marine environment locatio... more A total of sixty eight actinomycetes were isolated from near sea shore marine environment locations of Bigeum Island, South West coast of South Korea. The majority of these isolates were assigned to the genus Streptomyces of which one isolate showing broad spectrum of antimicrobial was on the basis of their morphological, physiological and biochemical properties. Phylogenetic analysis of the 16S rRNA gene sequence of one isolate e showed that strain was Streptomyces hygroscopicus BDUS 49 and it formed a distinct clad within the Streptomyces 16S rRNA gene tree closely related to Streptomyces hygroscopicus. This strain possessed a broad spectrum antimicrobial activity against Gram-positive,-& Gram-negative bacteria and fungi. The UV spectra of the active compounds in ethyl acetate showed peaks at between 200 to 295 nm. The bioactive region was detected on the TLC plate (R 0.40). The structure of the bioactive components was further f determined using FTIR, MS, C NMR and H NMR. The molecular formula of the given partially purified 13 1 compound being identified as 7, demethoxy rapamycin and their molecular formula derived a s C H NO +NA (MW 905.12).

Research paper thumbnail of Effect of Phenylacetic Acid Addition on Productivity of Penicillium chrysogenum in Penicillin G Production Using Pilot Scale Reactor

Effect of penicillin's precursor addition, phenylacetic acid (PAA), was done by addition of PAA i... more Effect of penicillin's precursor addition, phenylacetic acid (PAA), was done by addition of PAA in several concentrations in a pilot scale reactor batch culture to produce penicillin G with fungi Penicillium chrysogenum. Culture was analyzed by morphology observation and PAA was analyzed by HPLC. High concentration of PAA showed decreasing of biomassa and production of penicillin G that had contribution in increasing of cellular autolysis. However, Penicillium chrysogenum's morphology analysis did not show significant effect in autolysis and decreasing of biomassa. Low concentration of PAA showed low production of penicillin G and low effect in biomassa or autolysis. Those effects of PAA addition need an exploitation to induce the phenomenon in Penicillium chrysogenum's culture.

Research paper thumbnail of PEMANFAATAN LARVA LALAT BLACK SOLDIER FLY (Hermetia illucens) UNTUK PEMBUATAN PUPUK KOMPOS PADAT DAN PUPUK KOMPOS CAIR

JURNAL TECHLINK

Composting is a form of decomposing organic matter with the help of organisms for bioconversion. ... more Composting is a form of decomposing organic matter with the help of organisms for bioconversion. The composting process requires several requirements to produce good quality compost, namely water content, pH and nutrient availability which is reflected in the C / N ratio. One type of insect larvae that can be used as an element of organism is the Black Soldier Fly (BSF) insect. The effort to recycle organic waste involving Black Soldier Fly (BSF) insect larvae is a new paradigm in the management of organic waste as decomposers or decomposers of organic waste. The result of the conversion of organic waste into solid compost fertilizer has a moisture content value that exceeds quality standards, C-organic value and C / N ratio which is below the quality standard, has a basic pH, and macro nutrients (N + P2O5 + K2O) located in the Permentan quality standard No. 261 / KPTS / SR.30 / M /4/2019, while for liquid compost fertilizer the value of C-organic and macro nutrients (N + P2O5 + K2O...

Research paper thumbnail of Perbandingan Emisi Gas Buang Antara Motor Bahan Bakar Empat Tak Berbahan Bakar Premium, Pertalite, Dan Pertamax

JURNAL TECHLINK, Oct 1, 2023

Research paper thumbnail of Phytotoxic and Cytotoxic Effects, Antioxidant Potentials, and Phytochemical Constituents of <i>Stevia rebaudiana</i> Leaves

Research paper thumbnail of Isolation and screening of actinomycetes active against plant pathogenic fungi

AIP conference proceedings, 2024

Research paper thumbnail of Fermentation medium optimization of Streptomyces sp. as an antifungal agent against the Ganoderma boninensis pathogen in oil palm

Indonesian Journal of Biotechnology, Dec 29, 2023

Ganoderma boninensis is the most common fungus which attacks oil palm trees. However, a significa... more Ganoderma boninensis is the most common fungus which attacks oil palm trees. However, a significant percentage of inhibition to the problem is found through the use of Streptomyces sp. The optimization of the Streptomyces sp. fermentation medium growth factors affects the secondary metabolites production. This study aimed to identify the best formulation of carbon and nitrogen sources and the optimum concentration of Streptomyces sp. fermentation medium for antifungal compound production. The results showed that the best sources of carbon and nitrogen were liquid glucose and monosodium glutamate in the inhibition zones of 16.7 mm and 6.3 mm, while the best concentration levels were 20 g/L and 14.19 g/L, respectively. The results of the first optimization showed an inhibition zone response and area (%) of the optimum high-performance liquid chromatography (HPLC) chromatogram of 24.39 mm and 62.68 percent, respectively. Taking the suggestion of the first optimization, the second optimization produced 15.2 g/L and 8.3 g/L. The predicted value of the inhibition zone was 21.47 mm, and the area (%) of the HPLC chromatogram was 53.44 percent. The validation results showed an inhibition zone response of 22.01 mm and an HPLC chromatogram area (%) of 54.86 percent. The difference between the predicted and validation values was less than 5 percent; the validation value was thus in line with the value predicted by Design Expert 10.0.7. The chemical formula of the probable active compound is that of the cyclo(phenylalanyl-prolyl) compound.

Research paper thumbnail of Streptomyces costaricanus RS25 with bioactivity against plant pathogenic fungus

AIP Conference Proceedings, Dec 31, 2022

Research paper thumbnail of Isolation and Characterization of Thraustochytrium Trk-23 Producing Docosahexaenoic Acid from North Kalimantan, Indonesia

Pakistan Journal of Biological Sciences, Oct 14, 2023

Research paper thumbnail of Pengaruh Emisi Cerobong Boiler Batubara Terhadap Udara Ambien

JURNAL TECHLINK, Sep 24, 2023

Research paper thumbnail of Oxygen Depletion in a Faroese Sill Fjord

Research paper thumbnail of Chemometric analysis based on GC-MS chemical profiles of essential oil and extracts of black cumin (Nigella sativa L.) and their antioxidant potentials

journal of applied pharmaceutical science, Dec 31, 2022

Research paper thumbnail of EFEKTIVITAS CANGKANG BUAH PALA (Myristica fragrans) SEBAGAI KARBON AKTIF DALAM PENGOLAHAN AIR LIMBAH DOMESTIK

Activated carbon is a material that contains high carbon elements and has a porous solid. The mat... more Activated carbon is a material that contains high carbon elements and has a porous solid. The material used in this research is a nutmeg shell. The purpose of this study was to determine the effectiveness of activated charcoal from the shell of nutmeg (Myristica fragrans) as activated carbon in treating domestic wastewater. The variables studied to compare the effect of the wastewater absorption process are the concentration of the chemical NaOH 15% with temperature variations of 400ºC, 450ºC, and 500ºC. The best activation results were obtained at a temperature of 450ºC with a water content of 4.22%, an ash content of 10.24%, and iodine content of 577,395 mg/g. Activated charcoal parameters refer to SNI 06-3730-1995 regarding the quality standard requirements for activated charcoal. Wastewater management by activated charcoal from the shell of nutmeg (Myristica fragrans) with a concentration of 10, 15, 20 grams of activated charcoal. The best results were obtained by the concentration of activated charcoal 20 grams with a pH value of 9.0, TSS 0.663 mg/L, BOD 7.3 mg/L. For pH parameters, the effectiveness of activated charcoal is less than the maximum, and it does not meet quality standards.

Research paper thumbnail of Kemampuan Mikroba Konsorsium Untuk Aplikasi Pengolahan Limbah Tekstil

PROSIDING, Jul 19, 2022

Industri tekstil merupakan industri penyumbang devisa negara nomor tiga setelah pariwisata dan ke... more Industri tekstil merupakan industri penyumbang devisa negara nomor tiga setelah pariwisata dan kelapa sawit. Namun demikian industri tekstil di Indonesia dihadapkan pada permasalahan lingkungan dimana industri ini dikenal menghasilkan limbah yang cukup besar. Disamping menghasilkan zat pewarna, industri tekstil juga menghasilkan limbah cair dengan pH basa, mengandung fenol, logam berat, surfaktan dan beberapa jenis larutan penghilang kanji. Industri tekstil biasanya menghasilkan limbah dengan BOD yang cukup tinggi dan sebagian besar adalah limbah organik. Pengolahan limbah tekstil yang kaya akan limbah organik dengan BOD yang tinggi lebih tepat digunakan pengolahan limbah secara biologi dengan memanfaatkan mikroba konsorsium. Telah dilakukan uji kemampuan mikroba konsorsium yang terdiri dari Bacillus sp, Bacillus thuringiensis, Bacillus subtilis, dan Pseudomonas sp untuk pengolahan limbah tekstil. Penggunaan mikroba konsorsium ini mampu menurunkan kadar BOD dari 875 mg/L menjadi 95 mg/L, kadar COD 1875 mg/L menjadi 105 mg/L, SV30 menit dari 95% menjadi 85%, SV24 jam dari 67% menjadi 32%. Mikroba konsorsium ini berpotensi untuk dapat digunakan untuk pengolahan limbah dengan sistem lumpur aktif (active sludge).

Research paper thumbnail of Isolation, Identification, and Evaluation of Antimicrobial Activity of Active Compound Produced by Marine Actinomycetes isolate A32

ANNALES BOGORIENSES, Dec 18, 2015

The continuation of new antibiotics exploration becomes an important research program in the worl... more The continuation of new antibiotics exploration becomes an important research program in the world for pharmaceutical and agricultural applications. Marine filamentous bacteria such as actinomycetes have been widely used as an important biological tool to generate a variety of new secondary metabolites, such as antibiotic. The aim of this study was to obtain identified active compound and determine its antimicrobial activity. Isolation, identification, and antimicrobial activity assay of active compound produced by marine actinomycetes isolate A32 had been conducted. Production of active compound using isolate actinomycetes A32 was conducted involving glucose, yeast, peptone medium. The fermentation was carried out at 30 ºC for 5 days. The broth of supernatant was extracted using ethyl acetate. Purification of active compound used chromatography column and eluted stepwise with the chloroform and methanol solvents. Antimicrobial activity was monitored using agar disc diffusion, and microbial test was conducted by analyzing the samples diameter of clear zone towards Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 66923, and Candida albican BIOMCC 00122. Results of isolation and purification of active compound produced by actinomycetes isolate A32 show that this compound has a molecular weight of 503.1 g/mol with molecular formula C 26 H 37 N 3 O 7. Furthermore, this compound was suspected as Madumycin II after analysis of spectrum using 1 HNMR and COSY. The antimicrobial activity assay confirms that this active compound inhibited the growth of Staphylococcus aureus ATCC 25923 and Candida albican BIOMCC 00122.

Research paper thumbnail of PENGARUH WAKTU AERASI TERHADAP LIMBAH KEDELAI MENGGUNAKAN BAKTERI Pseudomonas Putida

Industrial liquid waste in liquid form resulting from tempe production can pollute the environmen... more Industrial liquid waste in liquid form resulting from tempe production can pollute the environment which is detrimental to the surrounding community. According to the World Health Organization (WHO), waste is the result of human activities that are useless, not used or something that is thrown into the environment. Tempe industrial wastewater is very disturbing to waters if it is not managed properly before being discharged into water bodies because this waste causes an unpleasant odor and triggers the growth of various pathogenic bacteria. If the waste is directly disposed of into the waters, in a relatively short time it will cause a foul odor from H2S gas or ammonia as a result of the crying of the organic waste. The existence of a decomposition process will cause an unpleasant odor (Wiryani Erry, 2012). The process of making tempeh in each of its stages uses an average of water for washing, soaking, and boiling the soybeans. This means that quite a lot of liquid waste is produced. This study aims to analyze the content of soybean liquid waste to reduce BOD levels to make it more environmentally friendly. With the aim of reducing BOD levels, this study will use the Pseudomonas Putida bacterium as a waste decomposer, bioreceptors and microelectrodes as practical BOD measuring devices. The sample used is boiled liquid waste from soybean skin in the process of making tempe at one of the tempe entrepreneurs near the Satya Negara University of Indonesia. The sample was then tested to see the content of DO, BOD, temperature, pH, TDS, and total bacteria. The results of research on soybean liquid waste showed that the content of BOD and TDS exceeded the quality standards set by the Regulation of the State Minister for the Environment 03 of 2010 concerning the Environment.

Research paper thumbnail of EFEKTIVITAS CANGKANG BUAH PALA (Myristica fragrans) SEBAGAI KARBON AKTIF DALAM PENGOLAHAN AIR LIMBAH DOMESTIK

JURNAL TECHLINK

Activated carbon is a material that contains high carbon elements and has a porous solid. The mat... more Activated carbon is a material that contains high carbon elements and has a porous solid. The material used in this research is a nutmeg shell. The purpose of this study was to determine the effectiveness of activated charcoal from the shell of nutmeg (Myristica fragrans) as activated carbon in treating domestic wastewater. The variables studied to compare the effect of the wastewater absorption process are the concentration of the chemical NaOH 15% with temperature variations of 400ºC, 450ºC, and 500ºC. The best activation results were obtained at a temperature of 450ºC with a water content of 4.22%, an ash content of 10.24%, and iodinecontent of 577,395 mg/g. Activated charcoal parameters refer to SNI 06-3730-1995 regarding the quality standard requirements for activated charcoal. Wastewater management by activated charcoal from the shell of nutmeg (Myristica fragrans) with a concentration of 10, 15, 20 grams of activated charcoal. The best results were obtained by the concentrati...

Research paper thumbnail of Isolation, Purification, and Characterization of Antimicrobial Substances from Endophytic Actinomycetes

Makara Seri Sains, Feb 28, 2014

Antimicrobial active substances produced by endophytic actinomycetes were isolated and purified. ... more Antimicrobial active substances produced by endophytic actinomycetes were isolated and purified. Plant samples were obtained from four different medicinal plants namely Curcuma domestica, Phaleria macrocarpa, Isotoma longiflora, and Symplocos cocchinensis. Isolation of actinomycetes was conducted using HV agar with the addition of cycloheximide, nystatin, nalidixic acid, and rifamycin. A total of 21 actinomycete isolates were obtained and tested for antimicrobial activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, and Bacillus subtilis ATCC 66923. Among the isolates, isolate KY01 was the most active to Gram-positive and Gram-negative bacteria. Morphological observation and identification using 16S rRNA gene sequence showed that the isolate KY01 was similar to Streptomyces antibioticus. An active compound from the isolate KY01 was produced using yeast peptone medium. The active compound was purified using silica-gel-column chromatography and preparative high performance liquid chromatography (HPLC). A single peak of the active compound was detected with HPLC and LCMS, which showed a retention time of 26.6 min and molecular weight (MW) 906.4474 g/mol, respectively. Abstrak Isolasi, Purifikasi, dan Karakterisasi Senyawa Antimikroba dari Aktinomisetes Endofit. Isolasi dan purifikasi senyawa aktif anti mikroba yang dihasilkan oleh aktinomisetes endofit telah dilakukan. Sampel tanaman diperoleh dari empat jenis tanaman obat yaitu Curcuma domestica, Phaleria macrocarpa, Isotoma longiflora, dan Symplocos cocchinensis. Isolasi aktinomistes endofit dilakukan dengan menggunakan medium HV agar dengan penambahan antibiotik sikloheksimida, nistatin, asam nalidik, dan rifamisin. Sebanyak 21 isolat aktinomisetes diperoleh dari sampel tanaman obat yang selanjutnya diuji aktivitas antimikroba terhadap Escherichia coli ATCC 25922, Staphylococcus aureus ATCC25923, Pseudomonas aeruginosa ATCC27853, dan Bacillus subtilis ATCC 66923. Hasil pengujian pada 21 isolat yang diperoleh, isolat KY01 menunjukkan aktivitas antimikroba paling kuat terhadap bakteri Gram-positif maupun bakteri Gram-negatif. Pengamatan secara morfologi dan identifikasi menggunakan sequence gen 16 rRNA menunjukkan bahwa isolat KY01 memiliki kemiripan dengan Streptomyces antibioticus. Produksi senyawa aktif isolat KY01 dengan kultur cair menggunakan medium yeast pepton. Purifikasi senyawa aktif dari ekstrak fermentasi dilakukan menggunakan kromatografi kolom silica gel dan HPLC preparatif. Hasil identifikasi menggunakan HPLC menunjukkan puncak dengan waktu retensi 26,6 menit dan hasil karakterisasi senyawa aktif menggunakan LCMS menunjukkan bobot molekul senyawa aktif sebesar (MW) 906,4474 g/mol.

Research paper thumbnail of Pengembangan Metode Analisis Benzena, Toluena, Dan Xylena Dalam Udara Lingkungan Kerja Menggunakan in House Metode DI PT Unilab Perdana

JURNAL TECHLINK

Benzene, Toluene and Xylene (BTX) compounds are compounds that have colorless and flammable chara... more Benzene, Toluene and Xylene (BTX) compounds are compounds that have colorless and flammable characteristics, have a sweet odor with a low toxicity level, and are not soluble in water. This compound is often used as a solvent, extractor and as a chemical intermediary. The testing of BTX parameters in the working environment refers to the National Institute of Occupational Safety and Health (NIOSH) method number 1501. The method must be modified or developed in terms of equipment and instruments, due to looking at the condition of the materials and equipment in PT UP, i.e. using different columns, capillary columns, fused silica, 30m x 0.32-mm ID; 1-μm film 100% PEG becomes a Rtx-5MS column, and measurements using a FID detector become a Mass Spectrometer detector. And the solvent used is Carbon Disulfiida (CS2) to Methanol for gas chromatography. CS2 solvents are carcinogenic in the body so that they are harmful to human health. Besides being harmful to health, the waste produced by ...

Research paper thumbnail of Cyclo (Tyrosyl-Prolyl) Produced by sp.: Bioactivity and Molecular Structure Elucidation Streptomyces

Pseudomonas aeruginosa Staphylococcus aureus Bacillus subtilis Streptomyces Streptomyces Escheric... more Pseudomonas aeruginosa Staphylococcus aureus Bacillus subtilis Streptomyces Streptomyces Escherichia coli Pseudomonas aeruginosa Staphylococcus aureus Bacillus subtilis Streptomyces Determination of bioactivity by minimum inhibitory concentration (MIC) methods and molecular structure identification of antibiotic produced by sp. have been carried out. The antibiotic was produced by liquid culture using sp. isolate. Purification of antibiotic was carried out by silica gel column chromatography and preparative HPLC. Molecular structure identification was carried out using ESI-MS, H NMR, C NMR, and C DEPT NMR. Pure antibiotic showed inhibition activity to Gram-negative and Gram-positive bacteria. MIC to ATCC 25922,

Research paper thumbnail of Isolation and Characterization of Antimicrobial Substance from Marine Streptomyces sp

Microbiology Indonesia, Aug 1, 2010

A total of sixty eight actinomycetes were isolated from near sea shore marine environment locatio... more A total of sixty eight actinomycetes were isolated from near sea shore marine environment locations of Bigeum Island, South West coast of South Korea. The majority of these isolates were assigned to the genus Streptomyces of which one isolate showing broad spectrum of antimicrobial was on the basis of their morphological, physiological and biochemical properties. Phylogenetic analysis of the 16S rRNA gene sequence of one isolate e showed that strain was Streptomyces hygroscopicus BDUS 49 and it formed a distinct clad within the Streptomyces 16S rRNA gene tree closely related to Streptomyces hygroscopicus. This strain possessed a broad spectrum antimicrobial activity against Gram-positive,-& Gram-negative bacteria and fungi. The UV spectra of the active compounds in ethyl acetate showed peaks at between 200 to 295 nm. The bioactive region was detected on the TLC plate (R 0.40). The structure of the bioactive components was further f determined using FTIR, MS, C NMR and H NMR. The molecular formula of the given partially purified 13 1 compound being identified as 7, demethoxy rapamycin and their molecular formula derived a s C H NO +NA (MW 905.12).

Research paper thumbnail of Effect of Phenylacetic Acid Addition on Productivity of Penicillium chrysogenum in Penicillin G Production Using Pilot Scale Reactor

Effect of penicillin's precursor addition, phenylacetic acid (PAA), was done by addition of PAA i... more Effect of penicillin's precursor addition, phenylacetic acid (PAA), was done by addition of PAA in several concentrations in a pilot scale reactor batch culture to produce penicillin G with fungi Penicillium chrysogenum. Culture was analyzed by morphology observation and PAA was analyzed by HPLC. High concentration of PAA showed decreasing of biomassa and production of penicillin G that had contribution in increasing of cellular autolysis. However, Penicillium chrysogenum's morphology analysis did not show significant effect in autolysis and decreasing of biomassa. Low concentration of PAA showed low production of penicillin G and low effect in biomassa or autolysis. Those effects of PAA addition need an exploitation to induce the phenomenon in Penicillium chrysogenum's culture.

Research paper thumbnail of PEMANFAATAN LARVA LALAT BLACK SOLDIER FLY (Hermetia illucens) UNTUK PEMBUATAN PUPUK KOMPOS PADAT DAN PUPUK KOMPOS CAIR

JURNAL TECHLINK

Composting is a form of decomposing organic matter with the help of organisms for bioconversion. ... more Composting is a form of decomposing organic matter with the help of organisms for bioconversion. The composting process requires several requirements to produce good quality compost, namely water content, pH and nutrient availability which is reflected in the C / N ratio. One type of insect larvae that can be used as an element of organism is the Black Soldier Fly (BSF) insect. The effort to recycle organic waste involving Black Soldier Fly (BSF) insect larvae is a new paradigm in the management of organic waste as decomposers or decomposers of organic waste. The result of the conversion of organic waste into solid compost fertilizer has a moisture content value that exceeds quality standards, C-organic value and C / N ratio which is below the quality standard, has a basic pH, and macro nutrients (N + P2O5 + K2O) located in the Permentan quality standard No. 261 / KPTS / SR.30 / M /4/2019, while for liquid compost fertilizer the value of C-organic and macro nutrients (N + P2O5 + K2O...