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Papers by s Ayyappa
Indian Journal of Fisheries, 2004
Actinomycin D and cycloheximide were used as inhibitors to investigate their effect in the oocyte... more Actinomycin D and cycloheximide were used as inhibitors to investigate their effect in the oocyte maturation of Catla catla. Full grown immature oocytes incubated with salmon gonadotropin (SG-G100) and 17α-20β-dihydroxy-4pregnen-3-one (17α-20β-DP) at the concentration of 1 µg/ml induced 91.7 + 1.9 and 93.7 + 2.6 of germinal vesicle breakdown (GVBD) respectively. A significant decline in the frequency of GVBD was observed when the oocytes were incubated with SG-G100 (1 µg/ml) alongwith different concentrations of actinomycin D or cycloheximide. This suggests that both the transcriptional as well as translational inhibitors inhibit the gonadotropin-induced oocyte maturation. A significant inhibition was also observed when the oocytes were incubated in combination with 17α-20β-DP (1 µg/ml) and different concentrations of cycloheximide. The oocyte maturation was not inhibited when the oocytes were incubated in the presence of 17α-20β-DP (1 µg/ml) and different concentrations of actinomycin D, which suggests that mRNA synthesis is not obligatory for 17α-20β-DP-induced oocyte maturation. It was also found that the inhibition of oocyte maturation in cycloheximide treated oocytes somehow extends up to 12 h after which the effects gradually declined.
Indian Journal of Fisheries, 2011
Actinomycin D and cycloheximide were used as inhibitors to investigate their effect in the oocyte... more Actinomycin D and cycloheximide were used as inhibitors to investigate their effect in the oocyte maturation of Catla catla. Full grown immature oocytes incubated with salmon gonadotropin (SG-G100) and 17α-20β-dihydroxy-4pregnen-3-one (17α-20β-DP) at the concentration of 1 µg/ml induced 91.7 + 1.9 and 93.7 + 2.6 of germinal vesicle breakdown (GVBD) respectively. A significant decline in the frequency of GVBD was observed when the oocytes were incubated with SG-G100 (1 µg/ml) alongwith different concentrations of actinomycin D or cycloheximide. This suggests that both the transcriptional as well as translational inhibitors inhibit the gonadotropin-induced oocyte maturation. A significant inhibition was also observed when the oocytes were incubated in combination with 17α-20β-DP (1 µg/ml) and different concentrations of cycloheximide. The oocyte maturation was not inhibited when the oocytes were incubated in the presence of 17α-20β-DP (1 µg/ml) and different concentrations of actinomycin D, which suggests that mRNA synthesis is not obligatory for 17α-20β-DP-induced oocyte maturation. It was also found that the inhibition of oocyte maturation in cycloheximide treated oocytes somehow extends up to 12 h after which the effects gradually declined.
IJELLH (International Journal of English Language, Literature in Humanities), 2017
Sunetra Gupta was born in Calcutta in 1965 and is an established translator of the poetry of Rabi... more Sunetra Gupta was born in Calcutta in 1965 and is an established translator of the poetry of Rabindranath Tagore. She is a well known novelist, essayist and scientist. She is working as Professor of Theoretical Epidemiology at Oxford University in the Department of Zoology. From Princeton University she got graduation in 1987 and from the University of London she received Ph.D. in 1992. Her father, Dhruba Gupta had a profound influence on every view of her thinking
Indian Journal of Fisheries, 2004
Actinomycin D and cycloheximide were used as inhibitors to investigate their effect in the oocyte... more Actinomycin D and cycloheximide were used as inhibitors to investigate their effect in the oocyte maturation of Catla catla. Full grown immature oocytes incubated with salmon gonadotropin (SG-G100) and 17α-20β-dihydroxy-4pregnen-3-one (17α-20β-DP) at the concentration of 1 µg/ml induced 91.7 + 1.9 and 93.7 + 2.6 of germinal vesicle breakdown (GVBD) respectively. A significant decline in the frequency of GVBD was observed when the oocytes were incubated with SG-G100 (1 µg/ml) alongwith different concentrations of actinomycin D or cycloheximide. This suggests that both the transcriptional as well as translational inhibitors inhibit the gonadotropin-induced oocyte maturation. A significant inhibition was also observed when the oocytes were incubated in combination with 17α-20β-DP (1 µg/ml) and different concentrations of cycloheximide. The oocyte maturation was not inhibited when the oocytes were incubated in the presence of 17α-20β-DP (1 µg/ml) and different concentrations of actinomycin D, which suggests that mRNA synthesis is not obligatory for 17α-20β-DP-induced oocyte maturation. It was also found that the inhibition of oocyte maturation in cycloheximide treated oocytes somehow extends up to 12 h after which the effects gradually declined.
Indian Journal of Fisheries, 2011
Actinomycin D and cycloheximide were used as inhibitors to investigate their effect in the oocyte... more Actinomycin D and cycloheximide were used as inhibitors to investigate their effect in the oocyte maturation of Catla catla. Full grown immature oocytes incubated with salmon gonadotropin (SG-G100) and 17α-20β-dihydroxy-4pregnen-3-one (17α-20β-DP) at the concentration of 1 µg/ml induced 91.7 + 1.9 and 93.7 + 2.6 of germinal vesicle breakdown (GVBD) respectively. A significant decline in the frequency of GVBD was observed when the oocytes were incubated with SG-G100 (1 µg/ml) alongwith different concentrations of actinomycin D or cycloheximide. This suggests that both the transcriptional as well as translational inhibitors inhibit the gonadotropin-induced oocyte maturation. A significant inhibition was also observed when the oocytes were incubated in combination with 17α-20β-DP (1 µg/ml) and different concentrations of cycloheximide. The oocyte maturation was not inhibited when the oocytes were incubated in the presence of 17α-20β-DP (1 µg/ml) and different concentrations of actinomycin D, which suggests that mRNA synthesis is not obligatory for 17α-20β-DP-induced oocyte maturation. It was also found that the inhibition of oocyte maturation in cycloheximide treated oocytes somehow extends up to 12 h after which the effects gradually declined.
IJELLH (International Journal of English Language, Literature in Humanities), 2017
Sunetra Gupta was born in Calcutta in 1965 and is an established translator of the poetry of Rabi... more Sunetra Gupta was born in Calcutta in 1965 and is an established translator of the poetry of Rabindranath Tagore. She is a well known novelist, essayist and scientist. She is working as Professor of Theoretical Epidemiology at Oxford University in the Department of Zoology. From Princeton University she got graduation in 1987 and from the University of London she received Ph.D. in 1992. Her father, Dhruba Gupta had a profound influence on every view of her thinking