saba khan - Academia.edu (original) (raw)

Papers by saba khan

Experimental Dermatology, 2007

Abstract: Human tissue kallikreins are a family of 15 trypsin- or chymotrypsin-like secreted ser... more Abstract: Human tissue kallikreins are a family of 15 trypsin- or chymotrypsin-like secreted serine proteases (KLK1–KLK15). Many KLKs have been identified in normal stratum corneum (SC) and sweat, and are candidate desquamation-related proteases.We report quantification by enzyme-linked immunosorbent assay (ELISA) of KLK5, KLK6, KLK7, KLK8, KLK10, KLK11, KLK13 and KLK14 in the SC and serum of atopic dermatitis (AD) patients by ELISA, and examine their variation with clinical phenotype, correlation with blood levels of eosinophils, lactate dehydrogenase (LDH) and immunoglobulin E. The overall SC serine protease activities were also measured.In the SC of AD, all KLKs, except KLK11, were significantly elevated. The elevation of chymotrypsin-like KLK7 was predominant, compared with trypsin-like KLKs. The SC overall plasmin- and furin-like activities were significantly elevated, while trypsin- and chymotrypsin-like activities did not differ significantly. In the serum of AD patients, KLK8 was significantly elevated and KLK5 and KLK11 were significantly decreased. However, their serum levels were not modified by corticosteroid topical agents. The alterations of KLK levels in the SC of AD were more pronounced than those in the serum. KLK7 in the serum was significantly correlated with eosinophil counts in the blood of AD patients, while KLK5, KLK8 and KLK11 were significantly correlated with LDH in the serum.In conclusion, we report abnormal kallikrein levels in the SC and the serum of AD patients. KLKs might be involved in skin manifestation and/or focal/systemic inflammatory reactions in AD. Our data may contribute to a better understanding of the pathogenesis of AD.

Journal of Investigative Dermatology, 2006

Translational Research, 2011

Cell Biology and Toxicology, 2010

Implications of environmental toxins on the regulation of neutrophil function are being significa... more Implications of environmental toxins on the regulation of neutrophil function are being significantly appraised. Such effects can be varied and markedly different depending on the type and extent of chemical exposure, which results in direct damage to the immune system. Isocyanates with functional group (–NCO), are considered as highly reactive molecules with diverse industrial applications. However, patho-physiological implications resulting from their occupational and accidental exposures have not been well delineated. The present study was carried out to assess the immunotoxic response of isocyanates and their mode of action at a molecular level on cultured human neutrophils isolated from healthy human volunteers. Studies were conducted to evaluate both dose- and time-dependent (n = 3) response using N-succinimidyl N-methylcarbamate, a chemical entity that mimics the effects of methyl isocyanate in vitro. Measure of apoptosis through annexin-V-FITC/PI assay, active caspase-3, apoptotic DNA ladder assay and mitochondrial depolarization; induction of oxidative stress by CM-H2DCFDA and formation of 8′-hydroxy-2′-deoxyguanosine; and levels of antioxidant defense system enzyme glutathione reductase, multiplex cytometric bead array analysis to quantify the secreted cytokine levels (interleukin-8, interleukin-1β, interleukin-6, interleukin-10, interferon-γ, tumor necrosis factor, and interleukin-12p70) parameters were evaluated. Our results demonstrate that isocyanates induce neutrophil apoptosis via activation of mitochondrial-mediated pathway along with reactive oxygen species production; depletion in antioxidant defense states; and elevated pro-inflammatory cytokine response.

Cell Biology International, 2009

International Journal of Toxicology, 2011

Indian Journal of Clinical Biochemistry, 2011

The present study evaluated the plausible role of circulating biomarkers in immune pathogenesis o... more The present study evaluated the plausible role of circulating biomarkers in immune pathogenesis of chronic hepatitis considered a priority in clinical hepatology. Total viral load of chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) patients was quantified and correlation studies were performed with circulating levels of Th1/Th2 cytokines; C reactive protein and circulating nucleosomes; glutathione reductase (GR) and superoxide dismutase. To our knowledge, the study is first among its kind that validates strong positive correlation of viral load with IL-4, IL-6, GR in HBV and IL-6, IL-10, GR in HCV infections. Although, multi-centric studies including large cohorts are required for translating our findings to clinical practice, however, role of these biomarkers with potential diagnostic or prognostic significance might be helpful in clinical assessment of high-risk individuals, thereby, designing interventional strategies, towards development of personalized medicare. The results of our study also offer valuable insights of immune signaling mediators engaged in development of hepatocellular carcinoma.

Journal of Allergy and Clinical Immunology, 2005

Clinical and Experimental Allergy, 2007

Background Perennial rhinitis is common and often results in substantial, potentially avoidable, ... more Background Perennial rhinitis is common and often results in substantial, potentially avoidable, impairment of quality of life. Quality of rhinitis care has been shown to be sub-optimal in general practice. Rigorous evaluation of educational interventions for healthcare professionals using randomized controlled trials is very uncommon.Objective To evaluate the effectiveness of standardized allergy training for healthcare professionals on patients' disease-specific quality of life.Methods Patients with clinician diagnosed perennial rhinitis and/or a prescription for relevant nasal medication were recruited from general practice. Following baseline measurement of quality of life using the validated rhino-conjunctivitis questionnaire (RQLQ), patients were centrally randomized to receive care from an allergy-trained primary healthcare professional or routine care. RQLQ was measured again at 13 months after randomization.Results Process measures revealed that the training was well received. 202 patients were included in the intention-to-treat analysis and 157 in the per-protocol analysis. There was a 0.23 greater mean improvement in quality-of-life scores in the intervention group when compared with controls (P=0.08) in the intention-to-treat analysis, this increasing to a 0.3 greater mean improvement if confined to a per-protocol analysis (P=0.05). The intention-to-treat analysis showed that 39/101 (39%) in the intervention group showed a clinically significant 0.5 improvement in RQLQ compared with 28/101 (28%) of controls (risk difference=11%, number needed to treat=9, P=0.1).Conclusions Standardized allergy education given to primary healthcare professionals leads to modest improvements in disease-specific quality of life in patients with perennial rhinitis.

Journal of Allergy and Clinical Immunology, 2007

Journal of Allergy and Clinical Immunology, 2005

Monthly Notices of The Royal Astronomical Society, 2007

In a stationary, general relativistic, axisymmetric, inviscid and rotational accretion flow, desc... more In a stationary, general relativistic, axisymmetric, inviscid and rotational accretion flow, described within the Kerr geometric framework, transonicity has been examined by setting up the governing equations of the flow as a first-order autonomous dynamical system. The consequent linearized analysis of the critical points of the flow leads to a comprehensive mathematical prescription for classifying these points, showing that the only possibilities are saddle points and centre-type points for all ranges of values of the fixed flow parameters. The spin parameter of the black hole influences the multitransonic character of the flow, as well as some of its specific critical properties. The special case of a flow in the space-time of a non-rotating black hole, characterized by the Schwarzschild metric, has also been studied for comparison and the conclusions are compatible with what has been seen for the Kerr geometric case.

Monthly Notices of The Royal Astronomical Society, 2007

In a stationary, general relativistic, axisymmetric, inviscid and rotational accretion flow, desc... more In a stationary, general relativistic, axisymmetric, inviscid and rotational accretion flow, described within the Kerr geometric framework, transonicity has been examined by setting up the governing equations of the flow as a first-order autonomous dynamical system. The consequent linearised analysis of the critical points of the flow leads to a comprehensive mathematical prescription for classifying these points, showing that the only possibilities are saddle points and centre-type points for all ranges of values of the fixed flow parameters. The spin parameter of the black hole influences the multitransonic character of the flow, as well as some of its specific critical properties. The special case of a flow in the space-time of a non-rotating black hole, characterised by the Schwarzschild metric, has also been studied for comparison and the conclusions are compatible with what has been seen for the Kerr geometric case.

Experimental Dermatology, 2007

Abstract: Human tissue kallikreins are a family of 15 trypsin- or chymotrypsin-like secreted ser... more Abstract: Human tissue kallikreins are a family of 15 trypsin- or chymotrypsin-like secreted serine proteases (KLK1–KLK15). Many KLKs have been identified in normal stratum corneum (SC) and sweat, and are candidate desquamation-related proteases.We report quantification by enzyme-linked immunosorbent assay (ELISA) of KLK5, KLK6, KLK7, KLK8, KLK10, KLK11, KLK13 and KLK14 in the SC and serum of atopic dermatitis (AD) patients by ELISA, and examine their variation with clinical phenotype, correlation with blood levels of eosinophils, lactate dehydrogenase (LDH) and immunoglobulin E. The overall SC serine protease activities were also measured.In the SC of AD, all KLKs, except KLK11, were significantly elevated. The elevation of chymotrypsin-like KLK7 was predominant, compared with trypsin-like KLKs. The SC overall plasmin- and furin-like activities were significantly elevated, while trypsin- and chymotrypsin-like activities did not differ significantly. In the serum of AD patients, KLK8 was significantly elevated and KLK5 and KLK11 were significantly decreased. However, their serum levels were not modified by corticosteroid topical agents. The alterations of KLK levels in the SC of AD were more pronounced than those in the serum. KLK7 in the serum was significantly correlated with eosinophil counts in the blood of AD patients, while KLK5, KLK8 and KLK11 were significantly correlated with LDH in the serum.In conclusion, we report abnormal kallikrein levels in the SC and the serum of AD patients. KLKs might be involved in skin manifestation and/or focal/systemic inflammatory reactions in AD. Our data may contribute to a better understanding of the pathogenesis of AD.

Journal of Investigative Dermatology, 2006

Translational Research, 2011

Cell Biology and Toxicology, 2010

Implications of environmental toxins on the regulation of neutrophil function are being significa... more Implications of environmental toxins on the regulation of neutrophil function are being significantly appraised. Such effects can be varied and markedly different depending on the type and extent of chemical exposure, which results in direct damage to the immune system. Isocyanates with functional group (–NCO), are considered as highly reactive molecules with diverse industrial applications. However, patho-physiological implications resulting from their occupational and accidental exposures have not been well delineated. The present study was carried out to assess the immunotoxic response of isocyanates and their mode of action at a molecular level on cultured human neutrophils isolated from healthy human volunteers. Studies were conducted to evaluate both dose- and time-dependent (n = 3) response using N-succinimidyl N-methylcarbamate, a chemical entity that mimics the effects of methyl isocyanate in vitro. Measure of apoptosis through annexin-V-FITC/PI assay, active caspase-3, apoptotic DNA ladder assay and mitochondrial depolarization; induction of oxidative stress by CM-H2DCFDA and formation of 8′-hydroxy-2′-deoxyguanosine; and levels of antioxidant defense system enzyme glutathione reductase, multiplex cytometric bead array analysis to quantify the secreted cytokine levels (interleukin-8, interleukin-1β, interleukin-6, interleukin-10, interferon-γ, tumor necrosis factor, and interleukin-12p70) parameters were evaluated. Our results demonstrate that isocyanates induce neutrophil apoptosis via activation of mitochondrial-mediated pathway along with reactive oxygen species production; depletion in antioxidant defense states; and elevated pro-inflammatory cytokine response.

Cell Biology International, 2009

International Journal of Toxicology, 2011

Indian Journal of Clinical Biochemistry, 2011

The present study evaluated the plausible role of circulating biomarkers in immune pathogenesis o... more The present study evaluated the plausible role of circulating biomarkers in immune pathogenesis of chronic hepatitis considered a priority in clinical hepatology. Total viral load of chronic hepatitis B virus (HBV) and hepatitis C virus (HCV) patients was quantified and correlation studies were performed with circulating levels of Th1/Th2 cytokines; C reactive protein and circulating nucleosomes; glutathione reductase (GR) and superoxide dismutase. To our knowledge, the study is first among its kind that validates strong positive correlation of viral load with IL-4, IL-6, GR in HBV and IL-6, IL-10, GR in HCV infections. Although, multi-centric studies including large cohorts are required for translating our findings to clinical practice, however, role of these biomarkers with potential diagnostic or prognostic significance might be helpful in clinical assessment of high-risk individuals, thereby, designing interventional strategies, towards development of personalized medicare. The results of our study also offer valuable insights of immune signaling mediators engaged in development of hepatocellular carcinoma.

Journal of Allergy and Clinical Immunology, 2005

Clinical and Experimental Allergy, 2007

Background Perennial rhinitis is common and often results in substantial, potentially avoidable, ... more Background Perennial rhinitis is common and often results in substantial, potentially avoidable, impairment of quality of life. Quality of rhinitis care has been shown to be sub-optimal in general practice. Rigorous evaluation of educational interventions for healthcare professionals using randomized controlled trials is very uncommon.Objective To evaluate the effectiveness of standardized allergy training for healthcare professionals on patients' disease-specific quality of life.Methods Patients with clinician diagnosed perennial rhinitis and/or a prescription for relevant nasal medication were recruited from general practice. Following baseline measurement of quality of life using the validated rhino-conjunctivitis questionnaire (RQLQ), patients were centrally randomized to receive care from an allergy-trained primary healthcare professional or routine care. RQLQ was measured again at 13 months after randomization.Results Process measures revealed that the training was well received. 202 patients were included in the intention-to-treat analysis and 157 in the per-protocol analysis. There was a 0.23 greater mean improvement in quality-of-life scores in the intervention group when compared with controls (P=0.08) in the intention-to-treat analysis, this increasing to a 0.3 greater mean improvement if confined to a per-protocol analysis (P=0.05). The intention-to-treat analysis showed that 39/101 (39%) in the intervention group showed a clinically significant 0.5 improvement in RQLQ compared with 28/101 (28%) of controls (risk difference=11%, number needed to treat=9, P=0.1).Conclusions Standardized allergy education given to primary healthcare professionals leads to modest improvements in disease-specific quality of life in patients with perennial rhinitis.

Journal of Allergy and Clinical Immunology, 2007

Journal of Allergy and Clinical Immunology, 2005

Monthly Notices of The Royal Astronomical Society, 2007

In a stationary, general relativistic, axisymmetric, inviscid and rotational accretion flow, desc... more In a stationary, general relativistic, axisymmetric, inviscid and rotational accretion flow, described within the Kerr geometric framework, transonicity has been examined by setting up the governing equations of the flow as a first-order autonomous dynamical system. The consequent linearized analysis of the critical points of the flow leads to a comprehensive mathematical prescription for classifying these points, showing that the only possibilities are saddle points and centre-type points for all ranges of values of the fixed flow parameters. The spin parameter of the black hole influences the multitransonic character of the flow, as well as some of its specific critical properties. The special case of a flow in the space-time of a non-rotating black hole, characterized by the Schwarzschild metric, has also been studied for comparison and the conclusions are compatible with what has been seen for the Kerr geometric case.

Monthly Notices of The Royal Astronomical Society, 2007

In a stationary, general relativistic, axisymmetric, inviscid and rotational accretion flow, desc... more In a stationary, general relativistic, axisymmetric, inviscid and rotational accretion flow, described within the Kerr geometric framework, transonicity has been examined by setting up the governing equations of the flow as a first-order autonomous dynamical system. The consequent linearised analysis of the critical points of the flow leads to a comprehensive mathematical prescription for classifying these points, showing that the only possibilities are saddle points and centre-type points for all ranges of values of the fixed flow parameters. The spin parameter of the black hole influences the multitransonic character of the flow, as well as some of its specific critical properties. The special case of a flow in the space-time of a non-rotating black hole, characterised by the Schwarzschild metric, has also been studied for comparison and the conclusions are compatible with what has been seen for the Kerr geometric case.