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Papers by sagar mhaske

Research paper thumbnail of Biological parameters of stem borer Stromatium barbatum reared on grapevine wood logs under laboratory conditions

International Journal of Tropical Insect Science, 2021

Stem borer Stromatium barbatum has recently emerged as a serious pest problem of grapevines in In... more Stem borer Stromatium barbatum has recently emerged as a serious pest problem of grapevines in India. Hidden biology and lack of effective chemical control of stem borer warranted finding out weak links in its life cycle which aids in formulating effective management strategies. Being a monovoltine pest with annual life cycle, the systematic studies on developmental biology and stage specific mortality of stem borer in grapes are limited. In present study, a cohort life table was constructed for S. barbatum by rearing it on grapevine wood logs in the laboratory at 27 ± 1 ℃ temperature and 65 ± 5% RH. The life cycle of S. barbatum was tracked from egg hatching through larval and pupal stages till adult emergence and observations were recorded on stage specific developmental durations and mortality of immature stages, and longevity and fecundity of adult stages. The results revealed that the mean durations of egg, larvae and pupae were 6.60 ± 0.28 days, 296.10 ± 1.06 days and 19.70 ± 1.17 days, respectively. The adults after emerging from pupae could survive up to 36.40 ± 0.81 days. The egg hatching and adult emergence from pupae were 42.51% and 83.02%, respectively. S. barbatum population attained a net reproductive rate of 64.96 females/female/generation and a gross reproductive rate of 72.93 females/female/generation in her mean oviposition period of 9.80 ± 0.13 days. The data indicated that, S. barbatum population potentially might increase with an intrinsic rate (r m) of 0.087 females/female/day and a finite rate () of 1.09 females/ female/ day with a doubling time of 9.98 days and a mean generation time of 324.30 days. The mortality was relatively higher during the egg and larval stages, which was then gradually decreased for rest of the life span when S. babatum was reared on grapevine wood logs under laboratory conditions. Generation mortality for S. barbatum was 90.72%. The present study has generated detailed information on critical life events such as days to egg hatch, adult longevity and fecundity, time required for adults to oviposit after mating, and age-stage specific mortality. This could facilitate formulation and implementation of effective management strategies for S. barbatum in grape vineyards.

Research paper thumbnail of Isolation, identification and functional characterization of Escherichia coli as probiotic against Shigella in Bangladesh

Indian Journal of Microbiology Research, 2020

Background: Antibiotic resistance due to misuses of antibiotics is currently became potent threat... more Background: Antibiotic resistance due to misuses of antibiotics is currently became potent threat for treating patients suffering from infectious diseases in Bangladesh. It is the high time to develop some alternative placebo for the treatment of infectious diseases especially in Bangladesh. Probiotic especially E. coli could be that alternative choice of treatment against Shigella infection. Aims: The present study was undertaken to examine the potentiality of Escherichia coli as probiotic against Shigella. Materials and Methods: During this study, four poor hygienic areas around Chattogram City, Bangladesh were selected through survey and then human stool samples were collected. Isolation and identification of Escherichia coli were done with a combination of microbiological and PCR analysis. Probiotic activity of isolated E. coli against Shigella was determined by co-culture test. Results: Total eight isolates were identified as Escherichia coli by microbiological and biochemical test. All the isolates were also confirmed as bacteria, coliform as well as fecal coliform through PCR. In the probiotic activity test, all the identified isolates except one showed significant result as probiotic. Conclusion: This research identified the potentiality of Escherichia coli as probiotic to treat shigellosis in Bangladesh. The outcomes of this study might function as a strong background to develop Escherichia coli as probiotic against Shigella infection in Bangladesh.

Research paper thumbnail of Biology of Chrysoperla zastrowi sillemi (Esben-Petersen) on mealy bugs and aphids

Journal of Biological Control, 2017

The consumption capacity of 1 st , 2 nd , and 3 rd instar larvae of C. zastrowi sillemi on 2 nd i... more The consumption capacity of 1 st , 2 nd , and 3 rd instar larvae of C. zastrowi sillemi on 2 nd instar nymphs of different preys viz, Aphis gossypii, Phenacoccus solenopsis, Maconellicoccus hirsutus revealed that each predator larva consumed 353.37 A.

Research paper thumbnail of Development and consumption capacity of Chrysoperla carnea (Stephens) on mealy bugs and Aphids

Research paper thumbnail of First record of occurrence of Dervishiya cadambae on grapevine, Vitis vinifera, along with its morphological and molecular identification and pathogenicity evaluation potential of Metarhizium brunneum as its biocontrol agent

Bulletin of Insectology, 2020

Increasing demand for grapes (Vitis vinifera L.) worldwide makes it a high-value crop and also an... more Increasing demand for grapes (Vitis vinifera L.) worldwide makes it a high-value crop and also an important, valuable export commodity for India. In February 2016, a vineyard from Maharashtra, India was identified with entirely new insect damage symptoms from those observed earlier. After, further inspection, a new type of wood borer was noticed which was later identified as Dervishiya cadambae (Moore) (Lepidoptera Cossidae). D. cadambae is known as a major pest of Tectona grandis L.f. plantations in Kerala, Karnataka and Tamil Nadu states of India and causes extensive damage to the timber. Surveys were conducted in affected vineyards to assess the extent of infestation from 2016 to 2018. In 20 infested vineyards located in Sangli and Nashik districts of Maharashtra, 12-72% of grapevines were found to have active infestations. Young larvae fed under the bark and later instars bored inside and made galleries. D. cadambae caused extensive damage to the sapwood and heartwood of grapevi...

Research paper thumbnail of Compatibility of insecticides with Metarhizium brunneum (Petch) and Beauveria bassiana(Bals.) for bio-intensive management of pink mealybug, Maconellicoccus hirsutus (Green)in grapes

Journal of Biological Control, Sep 1, 2019

Grape (Vitis vinifera Linnaeus) is a high-value crop and important as a valuable export commodity... more Grape (Vitis vinifera Linnaeus) is a high-value crop and important as a valuable export commodity for India. Pink mealybug, Maconellicoccus hirsutus (Green) is one of the most important pests infesting grapes. Two entomopathogenic fungi were isolated from the field infected insects and were identified as Metarhizium brunneum (Petch) and Beauveria bassiana (Bals.). The pathogenicity study showed that both the fungi were capable of infecting M. hirsutus. LC 50 values 1.4 × 10 6 and 1.0 × 10 7 conidia per ml was recorded for M. brunneum and B. bassiana, respectively. Evaluation of compatibility of these fungi with insecticides is important to develop bio-intensive management strategy for mealybugs. The compatibility of seven insecticides (emamectin benzoate, tolfenpyrad, imidacloprid, clothianidin, buprofezin, fipronil, spirotetramat) with these entomopathogens was evaluated under laboratory conditions. Compatibility studies based on sporulation, germination and vegetative growth of fungi showed that imidacloprid and emamectin benzoate were most compatible and tolfenpyrad and spirotetramat were highly incompatible with both the entomopathogens.

Research paper thumbnail of Biological parameters of stem borer Stromatium barbatum reared on grapevine wood logs under laboratory conditions

International Journal of Tropical Insect Science, 2021

Stem borer Stromatium barbatum has recently emerged as a serious pest problem of grapevines in In... more Stem borer Stromatium barbatum has recently emerged as a serious pest problem of grapevines in India. Hidden biology and lack of effective chemical control of stem borer warranted finding out weak links in its life cycle which aids in formulating effective management strategies. Being a monovoltine pest with annual life cycle, the systematic studies on developmental biology and stage specific mortality of stem borer in grapes are limited. In present study, a cohort life table was constructed for S. barbatum by rearing it on grapevine wood logs in the laboratory at 27 ± 1 ℃ temperature and 65 ± 5% RH. The life cycle of S. barbatum was tracked from egg hatching through larval and pupal stages till adult emergence and observations were recorded on stage specific developmental durations and mortality of immature stages, and longevity and fecundity of adult stages. The results revealed that the mean durations of egg, larvae and pupae were 6.60 ± 0.28 days, 296.10 ± 1.06 days and 19.70 ± 1.17 days, respectively. The adults after emerging from pupae could survive up to 36.40 ± 0.81 days. The egg hatching and adult emergence from pupae were 42.51% and 83.02%, respectively. S. barbatum population attained a net reproductive rate of 64.96 females/female/generation and a gross reproductive rate of 72.93 females/female/generation in her mean oviposition period of 9.80 ± 0.13 days. The data indicated that, S. barbatum population potentially might increase with an intrinsic rate (r m) of 0.087 females/female/day and a finite rate () of 1.09 females/ female/ day with a doubling time of 9.98 days and a mean generation time of 324.30 days. The mortality was relatively higher during the egg and larval stages, which was then gradually decreased for rest of the life span when S. babatum was reared on grapevine wood logs under laboratory conditions. Generation mortality for S. barbatum was 90.72%. The present study has generated detailed information on critical life events such as days to egg hatch, adult longevity and fecundity, time required for adults to oviposit after mating, and age-stage specific mortality. This could facilitate formulation and implementation of effective management strategies for S. barbatum in grape vineyards.

Research paper thumbnail of Isolation, identification and functional characterization of Escherichia coli as probiotic against Shigella in Bangladesh

Indian Journal of Microbiology Research, 2020

Background: Antibiotic resistance due to misuses of antibiotics is currently became potent threat... more Background: Antibiotic resistance due to misuses of antibiotics is currently became potent threat for treating patients suffering from infectious diseases in Bangladesh. It is the high time to develop some alternative placebo for the treatment of infectious diseases especially in Bangladesh. Probiotic especially E. coli could be that alternative choice of treatment against Shigella infection. Aims: The present study was undertaken to examine the potentiality of Escherichia coli as probiotic against Shigella. Materials and Methods: During this study, four poor hygienic areas around Chattogram City, Bangladesh were selected through survey and then human stool samples were collected. Isolation and identification of Escherichia coli were done with a combination of microbiological and PCR analysis. Probiotic activity of isolated E. coli against Shigella was determined by co-culture test. Results: Total eight isolates were identified as Escherichia coli by microbiological and biochemical test. All the isolates were also confirmed as bacteria, coliform as well as fecal coliform through PCR. In the probiotic activity test, all the identified isolates except one showed significant result as probiotic. Conclusion: This research identified the potentiality of Escherichia coli as probiotic to treat shigellosis in Bangladesh. The outcomes of this study might function as a strong background to develop Escherichia coli as probiotic against Shigella infection in Bangladesh.

Research paper thumbnail of Biology of Chrysoperla zastrowi sillemi (Esben-Petersen) on mealy bugs and aphids

Journal of Biological Control, 2017

The consumption capacity of 1 st , 2 nd , and 3 rd instar larvae of C. zastrowi sillemi on 2 nd i... more The consumption capacity of 1 st , 2 nd , and 3 rd instar larvae of C. zastrowi sillemi on 2 nd instar nymphs of different preys viz, Aphis gossypii, Phenacoccus solenopsis, Maconellicoccus hirsutus revealed that each predator larva consumed 353.37 A.

Research paper thumbnail of Development and consumption capacity of Chrysoperla carnea (Stephens) on mealy bugs and Aphids

Research paper thumbnail of First record of occurrence of Dervishiya cadambae on grapevine, Vitis vinifera, along with its morphological and molecular identification and pathogenicity evaluation potential of Metarhizium brunneum as its biocontrol agent

Bulletin of Insectology, 2020

Increasing demand for grapes (Vitis vinifera L.) worldwide makes it a high-value crop and also an... more Increasing demand for grapes (Vitis vinifera L.) worldwide makes it a high-value crop and also an important, valuable export commodity for India. In February 2016, a vineyard from Maharashtra, India was identified with entirely new insect damage symptoms from those observed earlier. After, further inspection, a new type of wood borer was noticed which was later identified as Dervishiya cadambae (Moore) (Lepidoptera Cossidae). D. cadambae is known as a major pest of Tectona grandis L.f. plantations in Kerala, Karnataka and Tamil Nadu states of India and causes extensive damage to the timber. Surveys were conducted in affected vineyards to assess the extent of infestation from 2016 to 2018. In 20 infested vineyards located in Sangli and Nashik districts of Maharashtra, 12-72% of grapevines were found to have active infestations. Young larvae fed under the bark and later instars bored inside and made galleries. D. cadambae caused extensive damage to the sapwood and heartwood of grapevi...

Research paper thumbnail of Compatibility of insecticides with Metarhizium brunneum (Petch) and Beauveria bassiana(Bals.) for bio-intensive management of pink mealybug, Maconellicoccus hirsutus (Green)in grapes

Journal of Biological Control, Sep 1, 2019

Grape (Vitis vinifera Linnaeus) is a high-value crop and important as a valuable export commodity... more Grape (Vitis vinifera Linnaeus) is a high-value crop and important as a valuable export commodity for India. Pink mealybug, Maconellicoccus hirsutus (Green) is one of the most important pests infesting grapes. Two entomopathogenic fungi were isolated from the field infected insects and were identified as Metarhizium brunneum (Petch) and Beauveria bassiana (Bals.). The pathogenicity study showed that both the fungi were capable of infecting M. hirsutus. LC 50 values 1.4 × 10 6 and 1.0 × 10 7 conidia per ml was recorded for M. brunneum and B. bassiana, respectively. Evaluation of compatibility of these fungi with insecticides is important to develop bio-intensive management strategy for mealybugs. The compatibility of seven insecticides (emamectin benzoate, tolfenpyrad, imidacloprid, clothianidin, buprofezin, fipronil, spirotetramat) with these entomopathogens was evaluated under laboratory conditions. Compatibility studies based on sporulation, germination and vegetative growth of fungi showed that imidacloprid and emamectin benzoate were most compatible and tolfenpyrad and spirotetramat were highly incompatible with both the entomopathogens.