sebastien tauzin - Academia.edu (original) (raw)

Papers by sebastien tauzin

Rubbia-Brandt et al. 1 Table S1: 613 up-regulated genes in SOS compared to control livers (FDR ad... more Rubbia-Brandt et al. 1 Table S1: 613 up-regulated genes in SOS compared to control livers (FDR adjusted p-value ≤ 0.05) Gene Symbol Name Fold-Chang e (SOS vs. Contro l) p-value (SOS vs. Control) adjusted p-value (SOS vs. Control)

International Journal of Immunopathology and Pharmacology, 2010

Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory-and bacterial-rela... more Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory-and bacterial-related diseases. The present study investigated in vitro and in vivo the effects of the stem bark methanol extract of S. birrea. Nitrite, TNF, IL-lP, IL-6 and IL-12p40 production by bone marrow-derived macrophages (BMDM) pre-incubated with or without S. birrea, and stimulated with Lipopolysaccharide (LPS) or infected with live Mycobacterium bovis Bacillus Calmette Guerin (BCG) was evaluated. S. birrea extract inhibited, in a concentration-dependent manner, nitrite, TNF, IL-lP, IL-6 and IL-12p40 production by BMDM stimulated with LPS or infected with live BCG. The iNOS expression was reduced by S. birrea after stimulation of BMDM with LPS. In addition, S. birrea inhibited the nuclear factor KB (NF-KB) activation by both LPS and BCG. The effects ofthe plant extract were also evaluated in an in vivo model of liver injury induced by D-galactosamine/LPS (D-GaINILPS) administration in mice. S. birrea limited D-GaIN/LPS-liver injury as assessed by a reduction in transaminases and TNF, IL-lP, IL-6 serum levels, and translocation of NF-KB to the nucleus. Taken together, our data indicate that stem bark methanol extract of S. birrea possesses anti-inflammatory properties by inhibiting NF-KB activation and cytokine release induced by inflammatory or infectious stimuli. Despite the technological advancement in modern medicine, approximately 80% of the populations in Africa still rely on traditional healing practices and medicinal plants for their health problems (1). Sclerocarya birrea (Anacardiaceae), commonly known as marula is a medium size, single stemmed tree which grows up to 20 m, with pale-grey bark flaking in patches and exposing underlying light yellow tissue. The bark is commonly used for the treatment of boils, diarrhoea and blood circulation problems, and mixed with other medicinal plants, is employed to treat rheumatism and infectious diseases such as syphilis, leprosy, dysentery and hepatitis (2).

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PLOS Biology, Feb 23, 2023

Acid Tetra-(acetoxymethyl) Ester]) and zVAD-fmk (carbobenzoxy-valyl-alanyl-aspartyl-[Omethyl]-flu... more Acid Tetra-(acetoxymethyl) Ester]) and zVAD-fmk (carbobenzoxy-valyl-alanyl-aspartyl-[Omethyl]-fluoromethylketone) were obtained from Calbiochem (Merck Chemicals Ltd., Nottingham, UK). DPX mountant, anti-β-actin, anti-tubulin, DAPI, diphenyleneiodonium (DPI), apocynin, N-acetyl L-cysteine (NAC) and anti-c-yes were purchased from Sigma (L'Isle-d'Abeau-Chesnes, France). Fura-2-PE3/AM and Fluo8-AM were from Tefflabs (Mundolsheim, France). Anti-human CD31 and D2-40 and H2FDA were from Invitrogen (Saint Aubin, France). Erlotinib, anti-CD95, anti-c-yes, anti-p22Phox and anti-nox4 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Cetuximab was obtained from the Centre Hospitalier de Rennes. Anti-caspase-8 (C15) was purchased from Axxora (Coger S.A., Paris, France). Anti-human CD95 mAb (DX2) and CD95L mAb (G247-4) were from BD Biosciences (Le Pont de Claix, France). Anti-human Orai1, Anti-Akt, anti-phosphoS473 Akt (Akt-P473), anti-p110α, anti-p110β, anti-EGF-R, anti-phosphoY845 EGFR (EGFR Y845) and anti-phospho(Y416) Src antibodies were from Cell Signaling Technology

PLOS Computational Biology

The motility of neutrophils and their ability to sense and to react to chemoattractants in their ... more The motility of neutrophils and their ability to sense and to react to chemoattractants in their environment are of central importance for the innate immunity. Neutrophils are guided towards sites of inflammation following the activation of G-protein coupled chemoattractant receptors such as CXCR2 whose signaling strongly depends on the activity of Ca2+ permeable TRPC6 channels. It is the aim of this study to analyze data sets obtained in vitro (murine neutrophils) and in vivo (zebrafish neutrophils) with a stochastic mathematical model to gain deeper insight into the underlying mechanisms. The model is based on the analysis of trajectories of individual neutrophils. Bayesian data analysis, including the covariances of positions for fractional Brownian motion as well as for exponentially and power-law tempered model variants, allows the estimation of parameters and model selection. Our model-based analysis reveals that wildtype neutrophils show pure superdiffusive fractional Brownia...

Scientific Reports

Tissue damage induces rapid recruitment of leukocytes and changes in the transcriptional landscap... more Tissue damage induces rapid recruitment of leukocytes and changes in the transcriptional landscape that influence wound healing. However, the cell-type specific transcriptional changes that influence leukocyte function and tissue repair have not been well characterized. Here, we employed translating ribosome affinity purification (TRAP) and RNA sequencing, TRAP-seq, in larval zebrafish to identify genes differentially expressed in neutrophils, macrophages, and epithelial cells in response to wounding. We identified the complement pathway and c3a.1, homologous to the C3 component of human complement, as significantly increased in neutrophils in response to wounds. c3a.1−/− zebrafish larvae have impaired neutrophil directed migration to tail wounds with an initial lag in recruitment early after wounding. Moreover, c3a.1−/− zebrafish larvae have impaired recruitment to localized bacterial infections and reduced survival that is, at least in part, neutrophil mediated. Together, our find...

Following acute injury, leukocytes rapidly infiltrate into tissues. For efficient recruitment, le... more Following acute injury, leukocytes rapidly infiltrate into tissues. For efficient recruitment, leukocytes must sense and respond to signals from both from the damaged tissue and from one another. However, the cell type specific transcriptional changes that influence leukocyte recruitment and wound healing have not been well characterized. In this study, we performed a large-scale translating ribosome affinity purification (TRAP) and RNA sequencing screen in larval zebrafish to identify genes differentially expressed by neutrophils, macrophages, and epithelial cells in the context of wounding. We identified the complement pathway and c3a.1, homologous to the C3A component of human complement, as significantly increased in neutrophils in response to a wound. We report that c3a.1−/− zebrafish larvae have impaired neutrophil responses to both tail wounds and localized bacterial infections, as well as increased susceptibility to infection due to a neutrophil-intrinsic function of C3A. We...

Journal of Hepatology, Dec 1, 2010

Background & Aims: Bacillus Calmette-Guérin (BCG) infection causes hepatic injury following granu... more Background & Aims: Bacillus Calmette-Guérin (BCG) infection causes hepatic injury following granuloma formation and secretion of cytokines which renders mice highly sensitive to endotoxin-mediated hepatotoxicity. Tumor necrosis factor (TNF) is required for granuloma formation and is one of the most important cytokines in liver injury. TNF inhibitors are effective therapies for inflammatory diseases. However, clinical use of nonselective TNF inhibitors is associated with an increased risk of infections. This work investigates the differential roles of soluble TNF (solTNF) and membrane TNF (memTNF) in BCG infection, BCG/LPS-and D-GALN/LPS-induced liver injury. Methods: We have used both genetic and pharmacologic approaches and analyzed liver injury, TLR4, cytokine and iNOS activation induced by BCG, BCG/LPS and D-GALN/LPS. Results: BCG infection-induced liver injury is seen in wild-type mice but not in TNF À/À , memTNF knock-in (KI), and sTNFR1-Fc transgenic mice. Severity of BCG-induced liver injury is correlated with BCG-granuloma number and hepatic expression of TLR4 and iNOS. In addition, protection from liver damage caused by BCG/ LPS or D-GALN/LPS administration was observed in TNF À/À , mem-TNF KI and sTNFR1-Fc transgenic mice. To extend the genetic findings, we then evaluated whether selective pharmacological inhibition of solTNF by dominant-negative (DN)-TNF neutralization and non-selective inhibition of solTNF and memTNF by anti-TNF antibodies and etanercept (TNFR2-IgG1) can protect the mice from liver injury. Both selective and non-selective inhibition of solTNF protected mice from BCG/LPS and D-GALN/LPSinduced liver damage. Conclusions: These data suggest that memTNF is not mediating liver injury and that selective inhibition of solTNF sparing mem-TNF may represent a new therapeutic strategy to treat immunemediated inflammatory liver diseases.

Scientific Reports, 2016

Transmembrane CD95L (Fas ligand) can be cleaved to release a promigratory soluble ligand, cl-CD95... more Transmembrane CD95L (Fas ligand) can be cleaved to release a promigratory soluble ligand, cl-CD95L, which can contribute to chronic inflammation and cancer cell dissemination. The motility signaling pathway elicited by cl-CD95L remains poorly defined. Here, we show that in the presence of cl-CD95L, CD95 activates the Akt and RhoA signaling pathways, which together orchestrate an allosteric activation of the Na + /H + exchanger NHE1. Pharmacologic inhibition of Akt or ROCK1 independently blocks the cl-CD95L-induced migration. Confirming these pharmacologic data, disruption of the Akt and ROCK1 phosphorylation sites on NHE1 decreases cell migration in cells exposed to cl-CD95L. Together, these findings demonstrate that NHE1 is a novel molecular actor in the CD95 signaling pathway that drives the cl-CD95L-induced cell migration through both the Akt and RhoA signaling pathways.

International journal of immunopathology and pharmacology

Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory- and bacterial-rel... more Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory- and bacterial-related diseases. The present study investigated in vitro and in vivo the effects of the stem bark methanol extract of S. birrea. Nitrite, TNF, IL-1beta, IL-6 and IL-12p40 production by bone marrow-derived macrophages (BMDM) pre-incubated with or without S. birrea, and stimulated with Lipopolysaccharide (LPS) or infected with live Mycobacterium bovis Bacillus Calmette Guérin (BCG) was evaluated. S. birrea extract inhibited, in a concentration-dependent manner, nitrite, TNF, IL-1beta, IL-6 and IL-12p40 production by BMDM stimulated with LPS or infected with live BCG. The iNOS expression was reduced by S. birrea after stimulation of BMDM with LPS. In addition, S. birrea inhibited the nuclear factor kB (NF-kB) activation by both LPS and BCG. The effects of the plant extract were also evaluated in an in vivo model of liver injury induced by D-galactosamine/LPS (D-GalN/LPS) administration in mic...

Communicative & Integrative Biology, 2012

Ca2+ entry, regulates recruitment of protein kinase C (PKC) b2, and prevents death-inducing signa... more Ca2+ entry, regulates recruitment of protein kinase C (PKC) b2, and prevents death-inducing signaling complex formation.

The Journal of cell biology, Jan 8, 2014

Tissue damage induces early recruitment of neutrophils through redox-regulated Src family kinase ... more Tissue damage induces early recruitment of neutrophils through redox-regulated Src family kinase (SFK) signaling in neutrophils. Redox-SFK signaling in epithelium is also necessary for wound resolution and tissue regeneration. How neutrophil-mediated inflammation resolves remains unclear. In this paper, we studied the interactions between macrophages and neutrophils in response to tissue damage in zebrafish and found that macrophages contact neutrophils and induce resolution via neutrophil reverse migration. We found that redox-SFK signaling through p22phox and Yes-related kinase is necessary for macrophage wound attraction and the subsequent reverse migration of neutrophils. Importantly, macrophage-specific reconstitution of p22phox revealed that macrophage redox signaling is necessary for neutrophil reverse migration. Thus, redox-SFK signaling in adjacent tissues is essential for coordinated leukocyte wound attraction and repulsion through pathways that involve contact-mediated gu...

B-non-Hodgkin lymphomas (B-NHLs) use a raft-associated signalosome made of the constitutively act... more B-non-Hodgkin lymphomas (B-NHLs) use a raft-associated signalosome made of the constitutively active Lyn kinase, the tyrosine phosphorylated Cbp/PAG adap- tor, and tyrosine phosphorylated STAT3 transcription factor. No such "signalo- some" is found in rafts of ALK T lym- phoma and Hodgkin-derived cell lines, despite similar Cbp/PAG, Lyn, and STAT3 expression and similar amounts of raft sphingolipids. Stable association of the

Rubbia-Brandt et al. 1 Table S1: 613 up-regulated genes in SOS compared to control livers (FDR ad... more Rubbia-Brandt et al. 1 Table S1: 613 up-regulated genes in SOS compared to control livers (FDR adjusted p-value ≤ 0.05) Gene Symbol Name Fold-Chang e (SOS vs. Contro l) p-value (SOS vs. Control) adjusted p-value (SOS vs. Control)

International Journal of Immunopathology and Pharmacology, 2010

Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory-and bacterial-rela... more Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory-and bacterial-related diseases. The present study investigated in vitro and in vivo the effects of the stem bark methanol extract of S. birrea. Nitrite, TNF, IL-lP, IL-6 and IL-12p40 production by bone marrow-derived macrophages (BMDM) pre-incubated with or without S. birrea, and stimulated with Lipopolysaccharide (LPS) or infected with live Mycobacterium bovis Bacillus Calmette Guerin (BCG) was evaluated. S. birrea extract inhibited, in a concentration-dependent manner, nitrite, TNF, IL-lP, IL-6 and IL-12p40 production by BMDM stimulated with LPS or infected with live BCG. The iNOS expression was reduced by S. birrea after stimulation of BMDM with LPS. In addition, S. birrea inhibited the nuclear factor KB (NF-KB) activation by both LPS and BCG. The effects ofthe plant extract were also evaluated in an in vivo model of liver injury induced by D-galactosamine/LPS (D-GaINILPS) administration in mice. S. birrea limited D-GaIN/LPS-liver injury as assessed by a reduction in transaminases and TNF, IL-lP, IL-6 serum levels, and translocation of NF-KB to the nucleus. Taken together, our data indicate that stem bark methanol extract of S. birrea possesses anti-inflammatory properties by inhibiting NF-KB activation and cytokine release induced by inflammatory or infectious stimuli. Despite the technological advancement in modern medicine, approximately 80% of the populations in Africa still rely on traditional healing practices and medicinal plants for their health problems (1). Sclerocarya birrea (Anacardiaceae), commonly known as marula is a medium size, single stemmed tree which grows up to 20 m, with pale-grey bark flaking in patches and exposing underlying light yellow tissue. The bark is commonly used for the treatment of boils, diarrhoea and blood circulation problems, and mixed with other medicinal plants, is employed to treat rheumatism and infectious diseases such as syphilis, leprosy, dysentery and hepatitis (2).

# ()))))))))))))))))))))))))))))))))))))))))))))))))))))))*+9 9 ,-&. &/% 9 " " &% 01 9 '. 2% 9 29... more # ()))))))))))))))))))))))))))))))))))))))))))))))))))))))*+9 9 ,-&. &/% 9 " " &% 01 9 '. 2% 9 29 3&2/4% #))))))))))))))))))))))))))))))))))))))))))))*+9 9 5&29 29 $%)))))))))))))))))))))))))))))))))))))))))))))))))))))))))))))))))))*+9

PLOS Biology, Feb 23, 2023

Acid Tetra-(acetoxymethyl) Ester]) and zVAD-fmk (carbobenzoxy-valyl-alanyl-aspartyl-[Omethyl]-flu... more Acid Tetra-(acetoxymethyl) Ester]) and zVAD-fmk (carbobenzoxy-valyl-alanyl-aspartyl-[Omethyl]-fluoromethylketone) were obtained from Calbiochem (Merck Chemicals Ltd., Nottingham, UK). DPX mountant, anti-β-actin, anti-tubulin, DAPI, diphenyleneiodonium (DPI), apocynin, N-acetyl L-cysteine (NAC) and anti-c-yes were purchased from Sigma (L'Isle-d'Abeau-Chesnes, France). Fura-2-PE3/AM and Fluo8-AM were from Tefflabs (Mundolsheim, France). Anti-human CD31 and D2-40 and H2FDA were from Invitrogen (Saint Aubin, France). Erlotinib, anti-CD95, anti-c-yes, anti-p22Phox and anti-nox4 were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Cetuximab was obtained from the Centre Hospitalier de Rennes. Anti-caspase-8 (C15) was purchased from Axxora (Coger S.A., Paris, France). Anti-human CD95 mAb (DX2) and CD95L mAb (G247-4) were from BD Biosciences (Le Pont de Claix, France). Anti-human Orai1, Anti-Akt, anti-phosphoS473 Akt (Akt-P473), anti-p110α, anti-p110β, anti-EGF-R, anti-phosphoY845 EGFR (EGFR Y845) and anti-phospho(Y416) Src antibodies were from Cell Signaling Technology

PLOS Computational Biology

The motility of neutrophils and their ability to sense and to react to chemoattractants in their ... more The motility of neutrophils and their ability to sense and to react to chemoattractants in their environment are of central importance for the innate immunity. Neutrophils are guided towards sites of inflammation following the activation of G-protein coupled chemoattractant receptors such as CXCR2 whose signaling strongly depends on the activity of Ca2+ permeable TRPC6 channels. It is the aim of this study to analyze data sets obtained in vitro (murine neutrophils) and in vivo (zebrafish neutrophils) with a stochastic mathematical model to gain deeper insight into the underlying mechanisms. The model is based on the analysis of trajectories of individual neutrophils. Bayesian data analysis, including the covariances of positions for fractional Brownian motion as well as for exponentially and power-law tempered model variants, allows the estimation of parameters and model selection. Our model-based analysis reveals that wildtype neutrophils show pure superdiffusive fractional Brownia...

Scientific Reports

Tissue damage induces rapid recruitment of leukocytes and changes in the transcriptional landscap... more Tissue damage induces rapid recruitment of leukocytes and changes in the transcriptional landscape that influence wound healing. However, the cell-type specific transcriptional changes that influence leukocyte function and tissue repair have not been well characterized. Here, we employed translating ribosome affinity purification (TRAP) and RNA sequencing, TRAP-seq, in larval zebrafish to identify genes differentially expressed in neutrophils, macrophages, and epithelial cells in response to wounding. We identified the complement pathway and c3a.1, homologous to the C3 component of human complement, as significantly increased in neutrophils in response to wounds. c3a.1−/− zebrafish larvae have impaired neutrophil directed migration to tail wounds with an initial lag in recruitment early after wounding. Moreover, c3a.1−/− zebrafish larvae have impaired recruitment to localized bacterial infections and reduced survival that is, at least in part, neutrophil mediated. Together, our find...

Following acute injury, leukocytes rapidly infiltrate into tissues. For efficient recruitment, le... more Following acute injury, leukocytes rapidly infiltrate into tissues. For efficient recruitment, leukocytes must sense and respond to signals from both from the damaged tissue and from one another. However, the cell type specific transcriptional changes that influence leukocyte recruitment and wound healing have not been well characterized. In this study, we performed a large-scale translating ribosome affinity purification (TRAP) and RNA sequencing screen in larval zebrafish to identify genes differentially expressed by neutrophils, macrophages, and epithelial cells in the context of wounding. We identified the complement pathway and c3a.1, homologous to the C3A component of human complement, as significantly increased in neutrophils in response to a wound. We report that c3a.1−/− zebrafish larvae have impaired neutrophil responses to both tail wounds and localized bacterial infections, as well as increased susceptibility to infection due to a neutrophil-intrinsic function of C3A. We...

Journal of Hepatology, Dec 1, 2010

Background & Aims: Bacillus Calmette-Guérin (BCG) infection causes hepatic injury following granu... more Background & Aims: Bacillus Calmette-Guérin (BCG) infection causes hepatic injury following granuloma formation and secretion of cytokines which renders mice highly sensitive to endotoxin-mediated hepatotoxicity. Tumor necrosis factor (TNF) is required for granuloma formation and is one of the most important cytokines in liver injury. TNF inhibitors are effective therapies for inflammatory diseases. However, clinical use of nonselective TNF inhibitors is associated with an increased risk of infections. This work investigates the differential roles of soluble TNF (solTNF) and membrane TNF (memTNF) in BCG infection, BCG/LPS-and D-GALN/LPS-induced liver injury. Methods: We have used both genetic and pharmacologic approaches and analyzed liver injury, TLR4, cytokine and iNOS activation induced by BCG, BCG/LPS and D-GALN/LPS. Results: BCG infection-induced liver injury is seen in wild-type mice but not in TNF À/À , memTNF knock-in (KI), and sTNFR1-Fc transgenic mice. Severity of BCG-induced liver injury is correlated with BCG-granuloma number and hepatic expression of TLR4 and iNOS. In addition, protection from liver damage caused by BCG/ LPS or D-GALN/LPS administration was observed in TNF À/À , mem-TNF KI and sTNFR1-Fc transgenic mice. To extend the genetic findings, we then evaluated whether selective pharmacological inhibition of solTNF by dominant-negative (DN)-TNF neutralization and non-selective inhibition of solTNF and memTNF by anti-TNF antibodies and etanercept (TNFR2-IgG1) can protect the mice from liver injury. Both selective and non-selective inhibition of solTNF protected mice from BCG/LPS and D-GALN/LPSinduced liver damage. Conclusions: These data suggest that memTNF is not mediating liver injury and that selective inhibition of solTNF sparing mem-TNF may represent a new therapeutic strategy to treat immunemediated inflammatory liver diseases.

Scientific Reports, 2016

Transmembrane CD95L (Fas ligand) can be cleaved to release a promigratory soluble ligand, cl-CD95... more Transmembrane CD95L (Fas ligand) can be cleaved to release a promigratory soluble ligand, cl-CD95L, which can contribute to chronic inflammation and cancer cell dissemination. The motility signaling pathway elicited by cl-CD95L remains poorly defined. Here, we show that in the presence of cl-CD95L, CD95 activates the Akt and RhoA signaling pathways, which together orchestrate an allosteric activation of the Na + /H + exchanger NHE1. Pharmacologic inhibition of Akt or ROCK1 independently blocks the cl-CD95L-induced migration. Confirming these pharmacologic data, disruption of the Akt and ROCK1 phosphorylation sites on NHE1 decreases cell migration in cells exposed to cl-CD95L. Together, these findings demonstrate that NHE1 is a novel molecular actor in the CD95 signaling pathway that drives the cl-CD95L-induced cell migration through both the Akt and RhoA signaling pathways.

International journal of immunopathology and pharmacology

Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory- and bacterial-rel... more Sclerocarya birrea is a medicinal plant used for the treatment of inflammatory- and bacterial-related diseases. The present study investigated in vitro and in vivo the effects of the stem bark methanol extract of S. birrea. Nitrite, TNF, IL-1beta, IL-6 and IL-12p40 production by bone marrow-derived macrophages (BMDM) pre-incubated with or without S. birrea, and stimulated with Lipopolysaccharide (LPS) or infected with live Mycobacterium bovis Bacillus Calmette Guérin (BCG) was evaluated. S. birrea extract inhibited, in a concentration-dependent manner, nitrite, TNF, IL-1beta, IL-6 and IL-12p40 production by BMDM stimulated with LPS or infected with live BCG. The iNOS expression was reduced by S. birrea after stimulation of BMDM with LPS. In addition, S. birrea inhibited the nuclear factor kB (NF-kB) activation by both LPS and BCG. The effects of the plant extract were also evaluated in an in vivo model of liver injury induced by D-galactosamine/LPS (D-GalN/LPS) administration in mic...

Communicative & Integrative Biology, 2012

Ca2+ entry, regulates recruitment of protein kinase C (PKC) b2, and prevents death-inducing signa... more Ca2+ entry, regulates recruitment of protein kinase C (PKC) b2, and prevents death-inducing signaling complex formation.

The Journal of cell biology, Jan 8, 2014

Tissue damage induces early recruitment of neutrophils through redox-regulated Src family kinase ... more Tissue damage induces early recruitment of neutrophils through redox-regulated Src family kinase (SFK) signaling in neutrophils. Redox-SFK signaling in epithelium is also necessary for wound resolution and tissue regeneration. How neutrophil-mediated inflammation resolves remains unclear. In this paper, we studied the interactions between macrophages and neutrophils in response to tissue damage in zebrafish and found that macrophages contact neutrophils and induce resolution via neutrophil reverse migration. We found that redox-SFK signaling through p22phox and Yes-related kinase is necessary for macrophage wound attraction and the subsequent reverse migration of neutrophils. Importantly, macrophage-specific reconstitution of p22phox revealed that macrophage redox signaling is necessary for neutrophil reverse migration. Thus, redox-SFK signaling in adjacent tissues is essential for coordinated leukocyte wound attraction and repulsion through pathways that involve contact-mediated gu...

B-non-Hodgkin lymphomas (B-NHLs) use a raft-associated signalosome made of the constitutively act... more B-non-Hodgkin lymphomas (B-NHLs) use a raft-associated signalosome made of the constitutively active Lyn kinase, the tyrosine phosphorylated Cbp/PAG adap- tor, and tyrosine phosphorylated STAT3 transcription factor. No such "signalo- some" is found in rafts of ALK T lym- phoma and Hodgkin-derived cell lines, despite similar Cbp/PAG, Lyn, and STAT3 expression and similar amounts of raft sphingolipids. Stable association of the