senewa pholoma - Academia.edu (original) (raw)

Papers by senewa pholoma

Research paper thumbnail of Characterizing the Agro-morphological Diversity of Corchorus olitorius L. Accessions in Botswana

Journal of experimental agriculture international, Mar 14, 2024

Aim: To evaluate the genetic diversity of the local and exotic accessions of this important crop.... more Aim: To evaluate the genetic diversity of the local and exotic accessions of this important crop. Study Design: The experiment was laid in a Random Complete Block Design (RCBD).

Research paper thumbnail of Assessment of Genetic Diversity in Corchorus olitorius Accessions by Simple Sequence Repeat Markers (SSR)

European of agriculture and food sciences, Nov 13, 2023

Indigenous leafy vegetables such as Corchorus olitorius (jew's mallow) have potential in contribu... more Indigenous leafy vegetables such as Corchorus olitorius (jew's mallow) have potential in contributing to food security as they can provide important nutritional requirements needed for human nourishment. However, in Botswana, this plant is not yet domesticated and its utilization is minimal due to lack of knowledge. To have the crop fully domesticated it is important to document important information on the available landraces and wild species. One of the important information is understanding the variation among the available genotypes as this will help not only in domestication but also in breeding purposes. Thus this study aimed to assess the genetic diversity of 49 accessions of jews mallow collected in Botswana and other African countries. Simple sequence repeats (SSR) markers were used to characterize fourty nine accessions. The results revealed that, out of 49 SSR primer pairs used, 46 showed scorable polymorphism by producing clear amplified products. The average polymorphic loci rate was 82.44% with the lowest rate (33.33%) detected in MJM-536 and the highest was 100% recorded by 27 of the primers. The polymorphism information content (PIC) potential ranged from 0.398 for MJM-475 primer to 0.979 for the primer MJM-623 with an average of 0.719. With the Shannon diversity index, an average of 3.626 was recorded under the studied accessions and this confirmed a very high diversity. In addition, a dendrogram was generated to illustrate the genetic diversity and possible relationships among the 49 Corchorus olitorius accessions using the unweighted pair group method (UPGM) with arithmetic means. This UPGM cluster analysis distinctively divided the accessions into five (5) groups at a cophenetic correlation coefficient 0.977. The total number of accessions per cluster varied from one group to the other. These results reinforced the effectiveness of the SSR markers in discriminating individuals within species even within a narrow genetic background.

Research paper thumbnail of Hot water treatment and duration alleviates chilling injury of mango (Mangifera indica L.)

Research paper thumbnail of Is Ethylene the Ripening Hormone

Journal of Experimental Agriculture International

The fruits constitute a commercially important and nutritionally indispensable food commodity sin... more The fruits constitute a commercially important and nutritionally indispensable food commodity since they play a vital role in human nutrition by supplying the necessary growth factors essential for maintaining normal health. One of the limiting factors that influence their economic value is the relatively short ripening period and reduced post-harvest life. The fruit ripening involves a wide spectrum of coordinated biochemical and physiological processes that eventually leads to development of soft edible fruit with desirable qualities such as carotenoids, anthocyanin, color, sweetness, texture, firmness, flavor and aroma. The ripening is the phase of fruit development just before senescence, therefore the excessive tissues softening due to the high ethylene exposure leads to the spoilage upon the storage. Ethylene, a fruit ripening hormone can trigger many events of cell metabolism including ripening particularly in climacteric fruits even in minute amounts. As fruit mature, t...

Research paper thumbnail of Effect of Hot Water Exposure Duration, Storage and Hot Water Temperature on Chilling Injury, Incidence and Quality of Keitt Mango (Mangifera indica L.)

Journal of Experimental Agriculture International

Low temperature storage is the most effective method of extending postharvest life and maintain f... more Low temperature storage is the most effective method of extending postharvest life and maintain fruit quality because it delays physiological processes such as ethylene production and senescence. Unfortunately, fruit such as mangoes are sensitive to low temperature storage and may be detrimental due to chilling injury, which reduces fruit quality. Effects of storage temperature, hot water at various temperatures and durations on alleviation of mango chilling injury and quality were evaluated on Keitt mango for the growing season in Botswana. The treatments were fruits dipped in distilled water at room temperature (25±2ºC- control), dipped in hot water at 50 and 55ºC for duration of 3, 5 and 10 minutes and storage temperatures at 4, 7, 10, 13 and 25±2ºC, plus 95% RH. The results showed that as storage temperature at below 13ºC, chilling injury incidence and severity significantly (P ≤ 0.0001) increased. Atwater temperature from 25ºC to 50 and 55ºC and duration in which mango fruit wa...

Research paper thumbnail of Physicochemical Attributes in Mango Fruit (Mangifera indica) as Influenced by Storage Temperature and Hot Water Treatment

Journal of Experimental Agriculture International, Feb 6, 2020

Background: The temperature being the most important environmental factor that influences the det... more Background: The temperature being the most important environmental factor that influences the deterioration of perishable commodities. It is often critical that fresh produce rapidly reach the optimal pulp temperature for short term storage if it is to maintain its highest visual quality, flavour, texture and nutritional content (Kader, 2013). Aims: The effects of storage temperature and hot water at various temperature and duration on chemical and textural characteristics of the Keitt mango fruit were evaluated for the 2015/16 growing season in Botswana. Materials and Methods: The treatments were fruits dipped in distilled water at room temperature (25±2ºC-control), fruits dipped in hot water at 50 and 55ºC for a duration of 3, 5 and 10 minutes, and storage temperatures at 4, 7, 10, 13, or 25±2ºC, plus 95% RH. Results: The results showed that as the storage temperature and water temperature decreased, the proline content and electrolyte leakage increased significantly (P ≤ 0.0001). The interactions of Original Research Article

Research paper thumbnail of Characterizing the Agro-morphological Diversity of Corchorus olitorius L. Accessions in Botswana

Journal of experimental agriculture international, Mar 14, 2024

Aim: To evaluate the genetic diversity of the local and exotic accessions of this important crop.... more Aim: To evaluate the genetic diversity of the local and exotic accessions of this important crop. Study Design: The experiment was laid in a Random Complete Block Design (RCBD).

Research paper thumbnail of Assessment of Genetic Diversity in Corchorus olitorius Accessions by Simple Sequence Repeat Markers (SSR)

European of agriculture and food sciences, Nov 13, 2023

Indigenous leafy vegetables such as Corchorus olitorius (jew's mallow) have potential in contribu... more Indigenous leafy vegetables such as Corchorus olitorius (jew's mallow) have potential in contributing to food security as they can provide important nutritional requirements needed for human nourishment. However, in Botswana, this plant is not yet domesticated and its utilization is minimal due to lack of knowledge. To have the crop fully domesticated it is important to document important information on the available landraces and wild species. One of the important information is understanding the variation among the available genotypes as this will help not only in domestication but also in breeding purposes. Thus this study aimed to assess the genetic diversity of 49 accessions of jews mallow collected in Botswana and other African countries. Simple sequence repeats (SSR) markers were used to characterize fourty nine accessions. The results revealed that, out of 49 SSR primer pairs used, 46 showed scorable polymorphism by producing clear amplified products. The average polymorphic loci rate was 82.44% with the lowest rate (33.33%) detected in MJM-536 and the highest was 100% recorded by 27 of the primers. The polymorphism information content (PIC) potential ranged from 0.398 for MJM-475 primer to 0.979 for the primer MJM-623 with an average of 0.719. With the Shannon diversity index, an average of 3.626 was recorded under the studied accessions and this confirmed a very high diversity. In addition, a dendrogram was generated to illustrate the genetic diversity and possible relationships among the 49 Corchorus olitorius accessions using the unweighted pair group method (UPGM) with arithmetic means. This UPGM cluster analysis distinctively divided the accessions into five (5) groups at a cophenetic correlation coefficient 0.977. The total number of accessions per cluster varied from one group to the other. These results reinforced the effectiveness of the SSR markers in discriminating individuals within species even within a narrow genetic background.

Research paper thumbnail of Hot water treatment and duration alleviates chilling injury of mango (Mangifera indica L.)

Research paper thumbnail of Is Ethylene the Ripening Hormone

Journal of Experimental Agriculture International

The fruits constitute a commercially important and nutritionally indispensable food commodity sin... more The fruits constitute a commercially important and nutritionally indispensable food commodity since they play a vital role in human nutrition by supplying the necessary growth factors essential for maintaining normal health. One of the limiting factors that influence their economic value is the relatively short ripening period and reduced post-harvest life. The fruit ripening involves a wide spectrum of coordinated biochemical and physiological processes that eventually leads to development of soft edible fruit with desirable qualities such as carotenoids, anthocyanin, color, sweetness, texture, firmness, flavor and aroma. The ripening is the phase of fruit development just before senescence, therefore the excessive tissues softening due to the high ethylene exposure leads to the spoilage upon the storage. Ethylene, a fruit ripening hormone can trigger many events of cell metabolism including ripening particularly in climacteric fruits even in minute amounts. As fruit mature, t...

Research paper thumbnail of Effect of Hot Water Exposure Duration, Storage and Hot Water Temperature on Chilling Injury, Incidence and Quality of Keitt Mango (Mangifera indica L.)

Journal of Experimental Agriculture International

Low temperature storage is the most effective method of extending postharvest life and maintain f... more Low temperature storage is the most effective method of extending postharvest life and maintain fruit quality because it delays physiological processes such as ethylene production and senescence. Unfortunately, fruit such as mangoes are sensitive to low temperature storage and may be detrimental due to chilling injury, which reduces fruit quality. Effects of storage temperature, hot water at various temperatures and durations on alleviation of mango chilling injury and quality were evaluated on Keitt mango for the growing season in Botswana. The treatments were fruits dipped in distilled water at room temperature (25±2ºC- control), dipped in hot water at 50 and 55ºC for duration of 3, 5 and 10 minutes and storage temperatures at 4, 7, 10, 13 and 25±2ºC, plus 95% RH. The results showed that as storage temperature at below 13ºC, chilling injury incidence and severity significantly (P ≤ 0.0001) increased. Atwater temperature from 25ºC to 50 and 55ºC and duration in which mango fruit wa...

Research paper thumbnail of Physicochemical Attributes in Mango Fruit (Mangifera indica) as Influenced by Storage Temperature and Hot Water Treatment

Journal of Experimental Agriculture International, Feb 6, 2020

Background: The temperature being the most important environmental factor that influences the det... more Background: The temperature being the most important environmental factor that influences the deterioration of perishable commodities. It is often critical that fresh produce rapidly reach the optimal pulp temperature for short term storage if it is to maintain its highest visual quality, flavour, texture and nutritional content (Kader, 2013). Aims: The effects of storage temperature and hot water at various temperature and duration on chemical and textural characteristics of the Keitt mango fruit were evaluated for the 2015/16 growing season in Botswana. Materials and Methods: The treatments were fruits dipped in distilled water at room temperature (25±2ºC-control), fruits dipped in hot water at 50 and 55ºC for a duration of 3, 5 and 10 minutes, and storage temperatures at 4, 7, 10, 13, or 25±2ºC, plus 95% RH. Results: The results showed that as the storage temperature and water temperature decreased, the proline content and electrolyte leakage increased significantly (P ≤ 0.0001). The interactions of Original Research Article