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Papers by sanjay ghodasara

International Journal of Current Microbiology and Applied Sciences, 2020

Infectious diseases are the leading contributors of mortality and morbidity in humans and animals... more Infectious diseases are the leading contributors of mortality and morbidity in humans and animals across the globe. Many emerging diseases, even epidemics are a direct consequence of zoonosis. Some infectious diseases like viral hemorrhagic fevers (VHFs) are a matter of great concern due to their zoonotic nature and associated fatality. VHFs are now becoming a severe threat to animals and humans. CrimeanCongo hemorrhagic fever (CCHF) is one of the most widely distributed viral hemorrhagic fevers among these (Mardani and Jahromi, 2007). It is an emerging zoonotic disease which has drawn attention of international community.

Toxicology and industrial health, 2015

The present study was carried out to evaluate the effects of exposure at different doses of aceph... more The present study was carried out to evaluate the effects of exposure at different doses of acephate on hematology, blood biochemistry, oxidative stress and immune system of Wistar rats. The experiment was carried out on 40 Wistar rats, which were divided in four groups. Animals of the three treatment groups were given with different sublethal doses (1/40th, 1/20th, 1/10th of lethal dose 50 value) of acephate by oral gavage. The hematology, blood biochemistry, oxidative stress marker, humoral immune response and cell-mediated immunity were evaluated following acephate exposure. Significant alteration in hematological parameters was not observed following different doses of acephate; however, significant alteration in alkaline phosphatase, gamma glutamyl transferase, acetyl cholinesterase, lipid peroxidase and superoxide dismutase was observed in medium- and high-dose group animals. Nonsignificant decrease in antibody titer in animals exposed to high dose has been observed compared w...

Lateral Flow Assay (LFA) considered as a field diagnostic test for brucellosis in small ruminant.... more Lateral Flow Assay (LFA) considered as a field diagnostic test for brucellosis in small ruminant. For the purpose, 268 whole blood samples (sheep-112 and goats-156) were collected from selected organized and scattered herds located in South Saurashtra region of Gujarat. Out of 112 sheep tested, 12 (10.71%), 8 (7.14%), 15 (13.39%), while from 156 goats tested, 24 (15.38%), 18 (11.54%), 25 (16.03%) were positive by Rose Bengal Plate Test (RBPT), Indirect Enzyme-Linked Immunosorbent Assay (iELISA) and LFA, respectively. The sensitivity of RBPT as compared to iELISA was 80.00% and 84.00%; and LFA as compared to iELISA was 53.33% and 64.00% in sera samples of sheep and goats, respectively. The specificity of RBPT and LFA was 100% in sheep for both these tests, whereas in goats sera samples it was 97.71% and 98.47% as compared to iELISA. The negative predictive value for RBPT was 97.00% and 96.97%; while for LFA it was 93.27% and 93.48% in sera samples of sheep and goats, respectively. Ho...

Buffalo Bulletin, 2010

Brucella could recovered in 10 samples of vaginal swabs, abortion and placenta (two from cattle, ... more Brucella could recovered in 10 samples of vaginal swabs, abortion and placenta (two from cattle, one from buffaloes, four from goats, and one from a bitch) of the 248 samples processed by cultural, morphological, biochemical characteristics. Among isolates, eight from abortion cases (two from cow, one from buffalo, four from goat and one from a bitch) and two isolates from reproductive disorder (one from a cow and one from a buffalo) were recovered. Three different sets of primers were compared for the detection of Brucella species. The three pairs of primers amplified three different fragments viz., (i) B4/B5 primer pair amplified a 223 bp (ii) F4/R2 primer pairs amplified a 905 bp (iii) JPF/JPR primer pair amplified a 193 bp. Of these primer pairs, B4/B5 was found to be more sensitive as it detected 10 Brucella isolates. Whereas the other two primer pairs, F4/R2 and JPF/JPR, detected eight samples of Brucella organisms. The isolates identified as Brucella organisms were subjected ...

Buffalo Bulletin (June 2010), 2010

Brucella could recovered in 10 samples of vaginal swabs, abortion and placenta (two from cattle, ... more Brucella could recovered in 10 samples of vaginal swabs, abortion and placenta (two from cattle, one from buffaloes, four from goats, and one from a bitch) of the 248 samples processed by cultural, morphological, biochemical characteristics. Among isolates, eight from abortion cases (two from cow, one from buffalo, four from goat and one from a bitch) and two isolates from reproductive disorder (one from a cow and one from a buffalo) were recovered. Three different sets of primers were compared for the detection of ...

International Journal of Current Microbiology and Applied Sciences, 2020

Infectious diseases are the leading contributors of mortality and morbidity in humans and animals... more Infectious diseases are the leading contributors of mortality and morbidity in humans and animals across the globe. Many emerging diseases, even epidemics are a direct consequence of zoonosis. Some infectious diseases like viral hemorrhagic fevers (VHFs) are a matter of great concern due to their zoonotic nature and associated fatality. VHFs are now becoming a severe threat to animals and humans. CrimeanCongo hemorrhagic fever (CCHF) is one of the most widely distributed viral hemorrhagic fevers among these (Mardani and Jahromi, 2007). It is an emerging zoonotic disease which has drawn attention of international community.

Toxicology and industrial health, 2015

The present study was carried out to evaluate the effects of exposure at different doses of aceph... more The present study was carried out to evaluate the effects of exposure at different doses of acephate on hematology, blood biochemistry, oxidative stress and immune system of Wistar rats. The experiment was carried out on 40 Wistar rats, which were divided in four groups. Animals of the three treatment groups were given with different sublethal doses (1/40th, 1/20th, 1/10th of lethal dose 50 value) of acephate by oral gavage. The hematology, blood biochemistry, oxidative stress marker, humoral immune response and cell-mediated immunity were evaluated following acephate exposure. Significant alteration in hematological parameters was not observed following different doses of acephate; however, significant alteration in alkaline phosphatase, gamma glutamyl transferase, acetyl cholinesterase, lipid peroxidase and superoxide dismutase was observed in medium- and high-dose group animals. Nonsignificant decrease in antibody titer in animals exposed to high dose has been observed compared w...

Lateral Flow Assay (LFA) considered as a field diagnostic test for brucellosis in small ruminant.... more Lateral Flow Assay (LFA) considered as a field diagnostic test for brucellosis in small ruminant. For the purpose, 268 whole blood samples (sheep-112 and goats-156) were collected from selected organized and scattered herds located in South Saurashtra region of Gujarat. Out of 112 sheep tested, 12 (10.71%), 8 (7.14%), 15 (13.39%), while from 156 goats tested, 24 (15.38%), 18 (11.54%), 25 (16.03%) were positive by Rose Bengal Plate Test (RBPT), Indirect Enzyme-Linked Immunosorbent Assay (iELISA) and LFA, respectively. The sensitivity of RBPT as compared to iELISA was 80.00% and 84.00%; and LFA as compared to iELISA was 53.33% and 64.00% in sera samples of sheep and goats, respectively. The specificity of RBPT and LFA was 100% in sheep for both these tests, whereas in goats sera samples it was 97.71% and 98.47% as compared to iELISA. The negative predictive value for RBPT was 97.00% and 96.97%; while for LFA it was 93.27% and 93.48% in sera samples of sheep and goats, respectively. Ho...

Buffalo Bulletin, 2010

Brucella could recovered in 10 samples of vaginal swabs, abortion and placenta (two from cattle, ... more Brucella could recovered in 10 samples of vaginal swabs, abortion and placenta (two from cattle, one from buffaloes, four from goats, and one from a bitch) of the 248 samples processed by cultural, morphological, biochemical characteristics. Among isolates, eight from abortion cases (two from cow, one from buffalo, four from goat and one from a bitch) and two isolates from reproductive disorder (one from a cow and one from a buffalo) were recovered. Three different sets of primers were compared for the detection of Brucella species. The three pairs of primers amplified three different fragments viz., (i) B4/B5 primer pair amplified a 223 bp (ii) F4/R2 primer pairs amplified a 905 bp (iii) JPF/JPR primer pair amplified a 193 bp. Of these primer pairs, B4/B5 was found to be more sensitive as it detected 10 Brucella isolates. Whereas the other two primer pairs, F4/R2 and JPF/JPR, detected eight samples of Brucella organisms. The isolates identified as Brucella organisms were subjected ...

Buffalo Bulletin (June 2010), 2010

Brucella could recovered in 10 samples of vaginal swabs, abortion and placenta (two from cattle, ... more Brucella could recovered in 10 samples of vaginal swabs, abortion and placenta (two from cattle, one from buffaloes, four from goats, and one from a bitch) of the 248 samples processed by cultural, morphological, biochemical characteristics. Among isolates, eight from abortion cases (two from cow, one from buffalo, four from goat and one from a bitch) and two isolates from reproductive disorder (one from a cow and one from a buffalo) were recovered. Three different sets of primers were compared for the detection of ...