shahram samiee - Academia.edu (original) (raw)

Papers by shahram samiee

Iranian journal of allergy, asthma and immunology, Feb 20, 2024

Scientific Journal of Iran Blood Transfus Organ, Feb 1, 2008

The Journal of Qazvin University of Medical Sciences, Feb 15, 2010

Background: The coagulation factor XIII is a pro-transglutaminase enzyme with tetrameric structur... more Background: The coagulation factor XIII is a pro-transglutaminase enzyme with tetrameric structure. An exchange of G for A in exon 2 of A subunit results in replacement of valine with leucine in amino acid 34. As a result of this substitution mutation, the clots produced are fragile and loose therefore it seems that FXIII Val34Leu polymorphism acts as a factor for individual protection against thrombosis Objectives: To determine the prevalence and role of FXIIIA Val34Leu polymorphism against deep vein thrombosis. Methods: This was a retrospective case-control study performed on 116 patients with DVT who were referred to Thrombosis and Homeostasis Laboratory affiliated to Iranian Blood Transfusion Organization. Also, 100 healthy individuals (blood donors) were recruited as control. Following DNA extraction and application of PCR and RFLP techniques in presence of restriction enzyme Cfo1, the genotypes of FXIII Val34Leu polymorphism were identified. The data were analyzed using chi square test as well as calculation of OD ratio and 95% confidence interval. Findings: The prevalence of FXIII Val34Leu polymorphism among the case and control groups was 22.4% and 37.4%, respectively. While the allele frequency of leucine in case group was 14.7% it was 20.2 % in control group. No significant correlation between polymorphism and sex was established. Conclusion: According to our data, no association between the FXIII Val34Leu polymorphism and protection against deep vein thrombosis was demonstrated. Therefore, it seems that this polymorphism occurs as a natural phenomenon and unaffected by gender.

Scientific Journal of Iran Blood Transfus Organ, Aug 1, 2009

The Medical Journal of The Islamic Republic of Iran, Aug 10, 2005

Background: Hepatitis B virus (HBV) infection in patients who lack detectable hepatitis B surface... more Background: Hepatitis B virus (HBV) infection in patients who lack detectable hepatitis B surface antigen (HBsAg) is called occult hepatitis B infection. Such infec tions have been frequently identified in patients with chronic hepatitis C liver disease, but their prevalence is not known. Methods: 207 patients with chronic hepatitis C who were HCV-RNA and anti-HCV positive were studied for HBV-DNA by PCR, and for HBsAg and anti-HBc by ELISA. DNA was extracted by high pure nucleic acid kit (Roche-Germany). HBV DNA amplification was done with a set of primer directed to the pre-S region. HBsAg and anti-HBc were evaluated by a commercially available ELISA kit (Dade Behring). Results: 23 of 207 patients with chronic hepatitis C liver disease (11.1%) were positive for HBV-DNA (co-infection). Among this group 17 patients (8.2%) were HBsAg negative (occult infection). 8 of 17 patients with occult infection (47%) were anti-HBc positive and 9 were anti-HBc negative (53%). No significant difference was found in epidemiological and biochemical param eters in patients with HCV alone in comparison with HCV co-infected with occult hepatitis B (p= 0.453 for ALT and p= 0.498 for AST). Conclusion: Occult hepatitis B virus infections occur frequently in patients with chronic hepatitis C liver disease and may have clinical significance.

new microbes and new infections, Nov 1, 2017

Materials and Methods An extra plasma sample was collected from 1026 blood donors. 1000 out of 10... more Materials and Methods An extra plasma sample was collected from 1026 blood donors. 1000 out of 1026 samples were negative for HBsAg, anti-HCV (EIA, third generation), anti-HIV and RPR. Every 5 samples were pooled. The sensitivity of HCV-RNA detection by RT-...

Scientific Journal of Iran Blood Transfus Organ, Sep 10, 2008

Scientific Journal of Iran Blood Transfus Organ, Jun 1, 2005

Scientific Journal of Iran Blood Transfus Organ, Jun 1, 2006

Materials and Methods: In this research, we studied the prevalence rate of TTV in 250 thalassemic... more Materials and Methods: In this research, we studied the prevalence rate of TTV in 250 thalassemic, hemophilic and dialysis patients and 250 blood donors. After extraction of DNA and amplification by seminested polymerase chain reaction, the bonds of DNA were ...

Scientific Journal of Iran Blood Transfus Organ, Sep 1, 2007

Journal of Thrombosis and Thrombolysis

<b>Copyright information:</b>Taken from "Serum hyaluronate as a non-invasive mar... more <b>Copyright information:</b>Taken from "Serum hyaluronate as a non-invasive marker of hepatic fibrosis and inflammation in HBeAg-negative chronic hepatitis B"BMC Gastroenterology 2005;5():32-32.Published online 12 Oct 2005PMCID:PMC1266362.Copyright © 2005 Montazeri et al; licensee BioMed Central Ltd. The box represents the interquartile range, the whiskers indicate the highest and lowest values, and the circles represent outliers. The line across the box indicates the median value.

Scientific Journal of Iran Blood Transfus Organ, 2017

Knowing the human platelet angens (HPA) and genes frequency in different populaons is important n... more Knowing the human platelet angens (HPA) and genes frequency in different populaons is important not only for populaon studies but also for clinical transfusion pracce. HPA genes frequency in Iran is not evaluated, so we invesgated the HPA-2, HPA-3, and HPA-5 frequency and their polymorphism. Materials and Methods: DNA from 120 Iranians (99 Iranian blood donors and 21 paents with platelet refractoriness) was isolated from peripheral blood mononuclear leucocytes and tested by restricted fragment length polymorphism analysis (RFLP). Results: The frequency of HPA phenotypes was determined as follows: (in donors) HPA-2a/2a:75.4%, HPA- 2a/2b:24.8%, HPA-3a/3a:8.1%, HPA-3a/3b:70.7%, HPA-3b/3b:21.2%, HPA-5a/5a:99 % and HPA-5a/5b:1%; (in paents) HPA-2a/2a:66.7%, HPA-2a/2b:33.3%, HPA-3a/3a:23.8%, HPA-3a/3b: 47.6%, HPA-3b/3b:28.6%, HPA-5a/5a:90.5%, and HPA-5a/5b: 9.5%. Frequency of genotypes evaluated by RFLP were as below: HPA-2a=0.87, HPA-2b=0.13, HPA- 3a =0.28, HPA-3b=0.72, HPA-5a=0.99, and ...

International Journal of Organ Transplantation Medicine, 2017

Background: Umbilical cord blood (UCB) is believed to be a highly valuable source of hematopoieti... more Background: Umbilical cord blood (UCB) is believed to be a highly valuable source of hematopoietic stem cells for transplantation. Objective: To investigate the prevalence of active and latent human cytomegalovirus (CMV) infection in UCB donors in Iranian population. Methods: A total of 825 UCB samples was collected under standard procedures and analyzed for the presence of CMV DNAs in buffy coat (latent infection) and plasma (active infection). DNA was extracted from buffy coat and plasma samples separately and tested with quantitative real-time PCR. All positive samples were checked by ELISA for IgG and IgM anti-CMV antibody. Results: Latent CMV infection was detected in 17 (2%) buffy coat samples with a low level of viral load, which indicated the presence of latent viral infection in donors. None of the plasma samples were found positive for CMV DNA reflecting no active infection. In the 17 positive samples, CMV viral load was 91–104 (mean: 100) copies/mL. All samples positive f...

Daneshvar, Sep 15, 2008

هديکچ همدقم فده و : مهم زا لماوع نيرت تيژننم هدننکدايجا کيتپسآ ياه يسوريو ، تـسا نوـيروا سوريو و ... more هديکچ همدقم فده و : مهم زا لماوع نيرت تيژننم هدننکدايجا کيتپسآ ياه يسوريو ، تـسا نوـيروا سوريو و سوريووترنا . زا باـنتجا يارـب نامرد يرورضيرغ ياه ، مزلا ساسـح و عيرـس يصـيخشت تسـت کي دوجو تسا . و داوم شور اه : RT-PCR هناگدنچ (Multiplex) ساسـح و عيرـس شور کي هب صيخشت يارب تنوفع عقوم رم باصعا متسيس يسوريو ياه تسا يزک . و نوــ يروا سورــ يو ود ناــ مزهم يياــ سانش يارــ ب شور نــ يا زا دش هدافتسا سوريووترنا . تست ادتبا PCR روـط هـب كـي ره يارب هنيبه هناگادج ادـج يارـب سپـس و يزاس سورـيو ود ناـمزهم يزاـس ديدرگ هدافتسا . هـب ينلوبولگ وركيم ود اتب نژ زا لترنـک ناوـنع دش هدافتسا يلخاد . هتفاي اه : 100 هنونم CSF ) يزغم عيام – يعانخ ( هـب کوکشـم راـميب عجم تيژننم تست و يروآ RT-PCR هناگدنچ نآ يور رب دـش ماـنجا اه . نونم ماتم ه اه عجم ي و دـندوب يـفنم ييايترکاب تشک رظن زا هدش يروآ مآ ناکدوک و نادازون زا زا رت 7 عجم لاس دوب هدش يروآ . 35 دصرد سوريووترنا رظن زا دراوم روـط هـب زـين دروم کي و دندوب تبثم دوب تبثم سوريو ود ره رظن زا نامزهم . هجيتن ييرگ : عيرس و ساسح شور کي تست نيا ) مآ رد زا رت 3 تعاـس ( تيژننم نامزهم صيخشت يارب يـم هـک تـسا کيتپسآ ياه نآ زا ناوـت درک هدافتسا زين کيژولويمديپا تاعلاطم يارب .

Background: Hepatitis B virus (HBV) infection in patients who lack detectable hepatitis B surface... more Background: Hepatitis B virus (HBV) infection in patients who lack detectable hepatitis B surface antigen (HBsAg) is called occult hepatitis B infection. Such infec­ tions have been frequently identified in patients with chronic hepatitis C liver disease, but their prevalence is not known. Methods: 207 patients with chronic hepatitis C who were HCV-RNA and anti-HCV positive were studied for HBV-DNA by PCR, and for HBsAg and anti-HBc by ELISA. DNA was extracted by high pure nucleic acid kit (Roche-Germany). HBV­ DNA amplification was done with a set of primer directed to the pre-S region. HBsAg and anti-HBc were evaluated by a commercially available ELISA kit (Dade Behring). Results: 23 of 207 patients with chronic hepatitis C liver disease (11.1%) were positive for HBV-DNA (co-infection). Among this group 17 patients (8.2%) were HBsAg negative (occult infection). 8 of 17 patients with occult infection (47%) were anti-HBc positive and 9 were anti-HBc negative (53%). No significant di...

Iranian journal of allergy, asthma and immunology, Feb 20, 2024

Scientific Journal of Iran Blood Transfus Organ, Feb 1, 2008

The Journal of Qazvin University of Medical Sciences, Feb 15, 2010

Background: The coagulation factor XIII is a pro-transglutaminase enzyme with tetrameric structur... more Background: The coagulation factor XIII is a pro-transglutaminase enzyme with tetrameric structure. An exchange of G for A in exon 2 of A subunit results in replacement of valine with leucine in amino acid 34. As a result of this substitution mutation, the clots produced are fragile and loose therefore it seems that FXIII Val34Leu polymorphism acts as a factor for individual protection against thrombosis Objectives: To determine the prevalence and role of FXIIIA Val34Leu polymorphism against deep vein thrombosis. Methods: This was a retrospective case-control study performed on 116 patients with DVT who were referred to Thrombosis and Homeostasis Laboratory affiliated to Iranian Blood Transfusion Organization. Also, 100 healthy individuals (blood donors) were recruited as control. Following DNA extraction and application of PCR and RFLP techniques in presence of restriction enzyme Cfo1, the genotypes of FXIII Val34Leu polymorphism were identified. The data were analyzed using chi square test as well as calculation of OD ratio and 95% confidence interval. Findings: The prevalence of FXIII Val34Leu polymorphism among the case and control groups was 22.4% and 37.4%, respectively. While the allele frequency of leucine in case group was 14.7% it was 20.2 % in control group. No significant correlation between polymorphism and sex was established. Conclusion: According to our data, no association between the FXIII Val34Leu polymorphism and protection against deep vein thrombosis was demonstrated. Therefore, it seems that this polymorphism occurs as a natural phenomenon and unaffected by gender.

Scientific Journal of Iran Blood Transfus Organ, Aug 1, 2009

The Medical Journal of The Islamic Republic of Iran, Aug 10, 2005

Background: Hepatitis B virus (HBV) infection in patients who lack detectable hepatitis B surface... more Background: Hepatitis B virus (HBV) infection in patients who lack detectable hepatitis B surface antigen (HBsAg) is called occult hepatitis B infection. Such infec tions have been frequently identified in patients with chronic hepatitis C liver disease, but their prevalence is not known. Methods: 207 patients with chronic hepatitis C who were HCV-RNA and anti-HCV positive were studied for HBV-DNA by PCR, and for HBsAg and anti-HBc by ELISA. DNA was extracted by high pure nucleic acid kit (Roche-Germany). HBV DNA amplification was done with a set of primer directed to the pre-S region. HBsAg and anti-HBc were evaluated by a commercially available ELISA kit (Dade Behring). Results: 23 of 207 patients with chronic hepatitis C liver disease (11.1%) were positive for HBV-DNA (co-infection). Among this group 17 patients (8.2%) were HBsAg negative (occult infection). 8 of 17 patients with occult infection (47%) were anti-HBc positive and 9 were anti-HBc negative (53%). No significant difference was found in epidemiological and biochemical param eters in patients with HCV alone in comparison with HCV co-infected with occult hepatitis B (p= 0.453 for ALT and p= 0.498 for AST). Conclusion: Occult hepatitis B virus infections occur frequently in patients with chronic hepatitis C liver disease and may have clinical significance.

new microbes and new infections, Nov 1, 2017

Materials and Methods An extra plasma sample was collected from 1026 blood donors. 1000 out of 10... more Materials and Methods An extra plasma sample was collected from 1026 blood donors. 1000 out of 1026 samples were negative for HBsAg, anti-HCV (EIA, third generation), anti-HIV and RPR. Every 5 samples were pooled. The sensitivity of HCV-RNA detection by RT-...

Scientific Journal of Iran Blood Transfus Organ, Sep 10, 2008

Scientific Journal of Iran Blood Transfus Organ, Jun 1, 2005

Scientific Journal of Iran Blood Transfus Organ, Jun 1, 2006

Materials and Methods: In this research, we studied the prevalence rate of TTV in 250 thalassemic... more Materials and Methods: In this research, we studied the prevalence rate of TTV in 250 thalassemic, hemophilic and dialysis patients and 250 blood donors. After extraction of DNA and amplification by seminested polymerase chain reaction, the bonds of DNA were ...

Scientific Journal of Iran Blood Transfus Organ, Sep 1, 2007

Journal of Thrombosis and Thrombolysis

<b>Copyright information:</b>Taken from "Serum hyaluronate as a non-invasive mar... more <b>Copyright information:</b>Taken from "Serum hyaluronate as a non-invasive marker of hepatic fibrosis and inflammation in HBeAg-negative chronic hepatitis B"BMC Gastroenterology 2005;5():32-32.Published online 12 Oct 2005PMCID:PMC1266362.Copyright © 2005 Montazeri et al; licensee BioMed Central Ltd. The box represents the interquartile range, the whiskers indicate the highest and lowest values, and the circles represent outliers. The line across the box indicates the median value.

Scientific Journal of Iran Blood Transfus Organ, 2017

Knowing the human platelet angens (HPA) and genes frequency in different populaons is important n... more Knowing the human platelet angens (HPA) and genes frequency in different populaons is important not only for populaon studies but also for clinical transfusion pracce. HPA genes frequency in Iran is not evaluated, so we invesgated the HPA-2, HPA-3, and HPA-5 frequency and their polymorphism. Materials and Methods: DNA from 120 Iranians (99 Iranian blood donors and 21 paents with platelet refractoriness) was isolated from peripheral blood mononuclear leucocytes and tested by restricted fragment length polymorphism analysis (RFLP). Results: The frequency of HPA phenotypes was determined as follows: (in donors) HPA-2a/2a:75.4%, HPA- 2a/2b:24.8%, HPA-3a/3a:8.1%, HPA-3a/3b:70.7%, HPA-3b/3b:21.2%, HPA-5a/5a:99 % and HPA-5a/5b:1%; (in paents) HPA-2a/2a:66.7%, HPA-2a/2b:33.3%, HPA-3a/3a:23.8%, HPA-3a/3b: 47.6%, HPA-3b/3b:28.6%, HPA-5a/5a:90.5%, and HPA-5a/5b: 9.5%. Frequency of genotypes evaluated by RFLP were as below: HPA-2a=0.87, HPA-2b=0.13, HPA- 3a =0.28, HPA-3b=0.72, HPA-5a=0.99, and ...

International Journal of Organ Transplantation Medicine, 2017

Background: Umbilical cord blood (UCB) is believed to be a highly valuable source of hematopoieti... more Background: Umbilical cord blood (UCB) is believed to be a highly valuable source of hematopoietic stem cells for transplantation. Objective: To investigate the prevalence of active and latent human cytomegalovirus (CMV) infection in UCB donors in Iranian population. Methods: A total of 825 UCB samples was collected under standard procedures and analyzed for the presence of CMV DNAs in buffy coat (latent infection) and plasma (active infection). DNA was extracted from buffy coat and plasma samples separately and tested with quantitative real-time PCR. All positive samples were checked by ELISA for IgG and IgM anti-CMV antibody. Results: Latent CMV infection was detected in 17 (2%) buffy coat samples with a low level of viral load, which indicated the presence of latent viral infection in donors. None of the plasma samples were found positive for CMV DNA reflecting no active infection. In the 17 positive samples, CMV viral load was 91–104 (mean: 100) copies/mL. All samples positive f...

Daneshvar, Sep 15, 2008

هديکچ همدقم فده و : مهم زا لماوع نيرت تيژننم هدننکدايجا کيتپسآ ياه يسوريو ، تـسا نوـيروا سوريو و ... more هديکچ همدقم فده و : مهم زا لماوع نيرت تيژننم هدننکدايجا کيتپسآ ياه يسوريو ، تـسا نوـيروا سوريو و سوريووترنا . زا باـنتجا يارـب نامرد يرورضيرغ ياه ، مزلا ساسـح و عيرـس يصـيخشت تسـت کي دوجو تسا . و داوم شور اه : RT-PCR هناگدنچ (Multiplex) ساسـح و عيرـس شور کي هب صيخشت يارب تنوفع عقوم رم باصعا متسيس يسوريو ياه تسا يزک . و نوــ يروا سورــ يو ود ناــ مزهم يياــ سانش يارــ ب شور نــ يا زا دش هدافتسا سوريووترنا . تست ادتبا PCR روـط هـب كـي ره يارب هنيبه هناگادج ادـج يارـب سپـس و يزاس سورـيو ود ناـمزهم يزاـس ديدرگ هدافتسا . هـب ينلوبولگ وركيم ود اتب نژ زا لترنـک ناوـنع دش هدافتسا يلخاد . هتفاي اه : 100 هنونم CSF ) يزغم عيام – يعانخ ( هـب کوکشـم راـميب عجم تيژننم تست و يروآ RT-PCR هناگدنچ نآ يور رب دـش ماـنجا اه . نونم ماتم ه اه عجم ي و دـندوب يـفنم ييايترکاب تشک رظن زا هدش يروآ مآ ناکدوک و نادازون زا زا رت 7 عجم لاس دوب هدش يروآ . 35 دصرد سوريووترنا رظن زا دراوم روـط هـب زـين دروم کي و دندوب تبثم دوب تبثم سوريو ود ره رظن زا نامزهم . هجيتن ييرگ : عيرس و ساسح شور کي تست نيا ) مآ رد زا رت 3 تعاـس ( تيژننم نامزهم صيخشت يارب يـم هـک تـسا کيتپسآ ياه نآ زا ناوـت درک هدافتسا زين کيژولويمديپا تاعلاطم يارب .

Background: Hepatitis B virus (HBV) infection in patients who lack detectable hepatitis B surface... more Background: Hepatitis B virus (HBV) infection in patients who lack detectable hepatitis B surface antigen (HBsAg) is called occult hepatitis B infection. Such infec­ tions have been frequently identified in patients with chronic hepatitis C liver disease, but their prevalence is not known. Methods: 207 patients with chronic hepatitis C who were HCV-RNA and anti-HCV positive were studied for HBV-DNA by PCR, and for HBsAg and anti-HBc by ELISA. DNA was extracted by high pure nucleic acid kit (Roche-Germany). HBV­ DNA amplification was done with a set of primer directed to the pre-S region. HBsAg and anti-HBc were evaluated by a commercially available ELISA kit (Dade Behring). Results: 23 of 207 patients with chronic hepatitis C liver disease (11.1%) were positive for HBV-DNA (co-infection). Among this group 17 patients (8.2%) were HBsAg negative (occult infection). 8 of 17 patients with occult infection (47%) were anti-HBc positive and 9 were anti-HBc negative (53%). No significant di...