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Papers by shalvi pandey
Plant science today, Dec 31, 2019
Groundnut, a major pulse crop, is prone to fungal diseases especially collar rot at seed germinat... more Groundnut, a major pulse crop, is prone to fungal diseases especially collar rot at seed germination and early seedling stage. In the present study, symbiotic bacteria was isolated and characterised from a collar rot resistant groundnut variety, Mallika and identified as Rhizobium pongamiae following biochemical and molecular characterization (NCBI Acc. No. MK770416). The bacterial isolate on cross inoculation, may be useful in improving crop productivity and enhancing collar rot disease tolerance in susceptible crops.
Journal of applied microbiology, Jan 16, 2017
The aim of this work was to isolate novel lignin degrading organisms. Several pure cultures of ba... more The aim of this work was to isolate novel lignin degrading organisms. Several pure cultures of bacteria that degrade lignin were isolated from bacterial consortia developed from decaying biomass. Among the isolates, Rhizobium sp. strain YS-1r (closest relative of Rhizobium petrolearium Strain SL-1) was explored for its lignin degrading ability. Microcosm studies showed that strain YS-1r was able to degrade a variety of lignin monomers, dimers, and also native lignin in switchgrass and alfalfa. The isolate demonstrated lignin peroxidase (Lip) activity when grown on alkali lignin, p-anisoin, switchgrass or alfalfa and only negligible activity was measured in glucose grown cells suggesting inducible nature of the Lip activity. Analysis of the strain YS-1r genome revealed the presence of a variety of genes that code for various lignin-oxidizing, H2 O2 producing, as well as polysaccharide hydrolyzing enzymes. This study shows both genomic and physiological capability of bacteria in the g...
International Journal of Plant & Soil Science
Groundnut, a major pulse crop also grown as an oil seed over 90 countries worldwide, is important... more Groundnut, a major pulse crop also grown as an oil seed over 90 countries worldwide, is important to the Indian economy. The states of Andhra Pradesh, Gujarat, Rajasthan and Tamil Nadu are the main producers of groundnut. In 2021, disease incidence with symptoms similar to collar rot was reported from Jaipur district of Rajasthan. The fungal strain was isolated from the collar region of susceptible groundnut variety TAG-24. White colonies with mycelia were observed in 3-4 days which eventually turned dark brown in color. Pathogenicity tests were performed on healthy groundnut and collar rot symptoms developed within 7-8 days of inoculation. Sequencing of PCR products amplified with the18S rRNA (NS1F-NS8R) and ITS (NS1F-ITS4R) primers indicated 99.4% and 100% identity respectively with a reference strain of Aspergillus niger CBS 554.65 (ATCC 16888) in the NCBI-Genebank database. Based on the morphological and molecular characteristics, the fungal pathogen was identified as Aspe...
Plant science today, Dec 31, 2019
Groundnut, a major pulse crop, is prone to fungal diseases especially collar rot at seed germinat... more Groundnut, a major pulse crop, is prone to fungal diseases especially collar rot at seed germination and early seedling stage. In the present study, symbiotic bacteria was isolated and characterised from a collar rot resistant groundnut variety, Mallika and identified as Rhizobium pongamiae following biochemical and molecular characterization (NCBI Acc. No. MK770416). The bacterial isolate on cross inoculation, may be useful in improving crop productivity and enhancing collar rot disease tolerance in susceptible crops.
Journal of applied microbiology, Jan 16, 2017
The aim of this work was to isolate novel lignin degrading organisms. Several pure cultures of ba... more The aim of this work was to isolate novel lignin degrading organisms. Several pure cultures of bacteria that degrade lignin were isolated from bacterial consortia developed from decaying biomass. Among the isolates, Rhizobium sp. strain YS-1r (closest relative of Rhizobium petrolearium Strain SL-1) was explored for its lignin degrading ability. Microcosm studies showed that strain YS-1r was able to degrade a variety of lignin monomers, dimers, and also native lignin in switchgrass and alfalfa. The isolate demonstrated lignin peroxidase (Lip) activity when grown on alkali lignin, p-anisoin, switchgrass or alfalfa and only negligible activity was measured in glucose grown cells suggesting inducible nature of the Lip activity. Analysis of the strain YS-1r genome revealed the presence of a variety of genes that code for various lignin-oxidizing, H2 O2 producing, as well as polysaccharide hydrolyzing enzymes. This study shows both genomic and physiological capability of bacteria in the g...
International Journal of Plant & Soil Science
Groundnut, a major pulse crop also grown as an oil seed over 90 countries worldwide, is important... more Groundnut, a major pulse crop also grown as an oil seed over 90 countries worldwide, is important to the Indian economy. The states of Andhra Pradesh, Gujarat, Rajasthan and Tamil Nadu are the main producers of groundnut. In 2021, disease incidence with symptoms similar to collar rot was reported from Jaipur district of Rajasthan. The fungal strain was isolated from the collar region of susceptible groundnut variety TAG-24. White colonies with mycelia were observed in 3-4 days which eventually turned dark brown in color. Pathogenicity tests were performed on healthy groundnut and collar rot symptoms developed within 7-8 days of inoculation. Sequencing of PCR products amplified with the18S rRNA (NS1F-NS8R) and ITS (NS1F-ITS4R) primers indicated 99.4% and 100% identity respectively with a reference strain of Aspergillus niger CBS 554.65 (ATCC 16888) in the NCBI-Genebank database. Based on the morphological and molecular characteristics, the fungal pathogen was identified as Aspe...