sharwan kumar - Academia.edu (original) (raw)

Papers by sharwan kumar

Lakes & Reservoirs: Science, Policy and Management for Sustainable Use

Present research study was conducted for a period of three months to evaluate each and every phas... more Present research study was conducted for a period of three months to evaluate each and every phase of industry’s waste water treatment processes as a cumulative research. The quantity and quality of brewery waste water fluctuates significantly, depending upon operations like raw material handling, wort preparation, fermentation, filtration, controls in process (CIP) and packaging. The water discharged is found highly organic in nature with high COD consisting of easily biodegradable sugars, soluble starch, ethanol and volatile fatty acids. The paper aims to study the performance evaluation of ETP of M/s Carlesberg India Ltd. a brewing industry located in Paonta Sahib, Himachal Pradesh, India. By feeding influent with controlled input parameters like pH, TSS and COD in UASB reactor, considerable reductions in the pollution loads were achieved. It was also found that improved removal efficiencies and lower electricity consumption are achieved by maintaining mixed liquor suspended solids (MLSS), dissolved oxygen (DO) and controlled oxygenation in the aerobic treatment. The performance evaluation has indicated that 96‐98% COD removal, 93‐98% TSS removal and 99% BOD removal efficiencies have been achieved. The replacement of existing Activated Sludge Process (ASP) with latest treatment technologies like i.e. Sequential Batch Reactor (SBR) and Membrane Process has been suggested as additional measures in order to achieve the target of less power and space requirement apart from meeting the effluent discharge standards in breweries.

Journal of Proteome Research

International Journal of Molecular Sciences

Nav1.5 is the pore forming α-subunit of the cardiac voltage-gated sodium channel that initiates c... more Nav1.5 is the pore forming α-subunit of the cardiac voltage-gated sodium channel that initiates cardiac action potential and regulates the human heartbeat. A normal level of Nav1.5 is crucial to cardiac function and health. Over- or under-expression of Nav1.5 can cause various cardiac diseases ranging from short PR intervals to Brugada syndromes. An assay that can directly quantify the protein amount in biological samples would be a priori to accurately diagnose and treat Nav1.5-associated cardiac diseases. Due to its large size (>200 KD), multipass transmembrane domains (24 transmembrane passes), and heavy modifications, Nav1.5 poses special quantitation challenges. To date, only the relative quantities of this protein have been measured in biological samples. Here, we describe the first targeted and mass spectrometry (MS)-based quantitative assay that can provide the copy numbers of Nav1.5 in cells with a well-defined lower limit of quantification (LLOQ) and precision. Applying...

National TRI, Aug 19, 2020

International Journal of Scientific Research in Computer Science, Engineering and Information Technology, 2021

The most important tool for a document examiner is his or her own vision, but in this section and... more The most important tool for a document examiner is his or her own vision, but in this section and the laboratory tour section we will consider the external tools which enable the examiner to see, evaluates and record more than is apparent to the unaided eye. The number of papers outlines the basic necessity of photographic illustrations for demonstrating the data offer clinching evidence, strong enough to with stand legal scrutiny on which opinions of the document examiner are based, hence play a very vital role in the examination of forensic documents. For the purpose of examination in the laboratory as well as the use of photographs becomes indispensible in cases involving traced or simulated forgery, built-up documents, forgery over genuine signatures and forgery by substitution, interpolations, differences in the tint of ink etc. The authors have solved a case in resolving the sequence of strokes with same ball point ink in an actual case received in the laboratory.

International Journal of Advances in Medicine, 2022

Background: Lung cancer is the most common cancer diagnosed worldwide. It is also the leading con... more Background: Lung cancer is the most common cancer diagnosed worldwide. It is also the leading contributor to cancer-related mortality, resulting in around 1.38 million cancer deaths per year worldwide. The aim and objective of the study was to study the clinical presentations including paraneoplastic manifestations and metastatic effects of lung cancer and to identify reasons for delay in diagnosis of lung cancer.Methods: Hospital based observational study was conducted on 100 consecutive cases.Results: Mean age of the cases with lung cancer was 56.36 years and 30% of the cases. Male predominance was seen with 63% males to 37% females. Tobacco smoking was present in 59% cases while exposure to second hand smoke and biomass fuel was seen in 11% and 9% patients. Most common presenting symptoms were cough (22.21%), chest pain (16.13%), fever (14.37%), dyspnea (11%) and hemoptysis (5.22%). Most common type of lung tumor was Adenocarcinoma (53%) followed by Small cell carcinoma (22%) and...

Aluminium industries require bulk power for their metal extraction and manufacturing process. Man... more Aluminium industries require bulk power for their metal extraction and manufacturing process. Manual operation and control of this process with large amount of power create technical difficulties and it becomes more difficult when process stations distributed over a large geographical area and smelter has the mix of diode rectifiers and thyristor rectifier systems. Hindalco has very large number of potlines and as well as a good mix of power supply. The smelter is being fed from a capative thermal power plant and connected with 132 KV grid interconnections. The auxiliary power for the smelter, aluminium plant and as well as rolling mills are being taken from same power lines feeding the smelting rectiformers. In case of grid disturbance, it was difficult to manage the network and if the system is island mode, the control of frequency and voltage were extremely difficult and the frequency used to go into the unstable region resulting in the tripping and black outs. To manage such bul...

INTERNATIONAL JOURNAL OF PLANT PROTECTION, 2017

Chilli (Capsicum annuum L.) is one of the most important vegetable among solanacious group like p... more Chilli (Capsicum annuum L.) is one of the most important vegetable among solanacious group like potato, tomato etc. Most of the promising chilli cultivars grown in nursery are under a great threat for profitable cultivation due to the attack of several abiotic and biotic factors viz., fungi, bacteria, virus and nematodes. The major losses of chilli in nursery stage are covered by fungi, in which damping-off and wilt disease of chilli caused by Fusarium oxysporum f.sp. capiscum is more important diseases. For the management of damping-off and wilt of chilli to produce healthy seedlings of Chilli free from damping-off and wilt disease, there was a need to develop new technology for better management in nursery stage an experiment was conducted at different places/ first at nursery and other in main field of Vegetable Research Farm for two years with ten treatments and three replications. The treatment of maximum seed germination (84.34%), root length (21.97cm), shoot length (16.19cm), viguor-index (3217.46), minimum damping-off disease incidence (6.49%), wilt disease incidence found in main field (0.00%) and maximum red ripe fruit yield 30.19 q/ha was recorded in (T 9) Fenamidone + mancozeb (0.25%) drenching, next best effective treatment were (T 6 a) seed treatment with (Carbendazim + mancozeb) 1.5g/kg seed + drenching of nursery (0.1%) and (T 7) use of Pencycuron 1ml/lit. in drenching which were statistically at par in case of seed germination, disease intensity andred ripe fruit.

Metanil yellow used as food colorant extensively used in various foods as an adulterant in differ... more Metanil yellow used as food colorant extensively used in various foods as an adulterant in different food substances such as turmeric, pulses, candy and ice-cream to provide a bright fresh colour to them. It belongs to non-permitted category of food colour as continued intake of this toxic food colour causes to adverse life –threatening effects in humans. Numerous studies conducted and reported the health issues related to metanil yellow. Awareness among general public should be increased regarding its toxic effects and use of metanil yellow in food materials should be completely banned.

International Journal of Research, 2017

Researches on electrostatics’ conceptions found that students have ideas and conceptions that dis... more Researches on electrostatics’ conceptions found that students have ideas and conceptions that disagree with the scientific models and that might explain students’ learning difficulties. To favour the change of student’s ideas and conceptions, a teaching sequence that relies on a historical study of electrostatics is proposed. It begins with an exploration of electrostatics phenomena that students would do with everyday materials. About these phenomena they must draw their own explanations that will be shared and discussed in the class. The teacher will collect and summarize the ideas and explanations which are nearer the history of science.

Lead is a heavy metal and a ubiquitous neurotoxin linked with learning and behavioural disorders.... more Lead is a heavy metal and a ubiquitous neurotoxin linked with learning and behavioural disorders. Lead exposure can cause learning, language and behavioural problems such as reduced IQ, impetuousness, extended aggression, lowered academic performance, anaemia, and with long exposure result in kidney failure. Lead is also associated with miscarriage, infertility in both sexes, changes in hormones, irregular menstruation and delays in the onset of puberty in girls.

Infection, 2021

Purpose Frequently the infection with coronavirus 2 (SARS-CoV-2) can be asymptomatic or provoke o... more Purpose Frequently the infection with coronavirus 2 (SARS-CoV-2) can be asymptomatic or provoke only mild symptoms. These cases often remain unnoticed, so it is difficult to estimate the actual numbers of infections. Aim of this study was to determine the seroprevalence of anti-SARS-CoV-2 total antibody in Austrian blood donors. Methods 20,228 blood donors aged between 18 and 72 years resident in four Austrian federal states were screened for anti-SARS-CoV-2 total antibody between 5th of June and 4th of December 2020. To evaluate the impact of sex, age, AB0-blood group and donation period on the anti-SARS-CoV-2 seroprevalence, multiple logistic regression was done. Results Our data reveal an anti-SARS-CoV-2 seroprevalence of 2.5% overall, significantly depending on the time point of blood donation: after the first Austrian lockdown the seroprevalence was lower compared to the following months, when the rate was constantly rising. While younger blood donors showed significantly highe...

Cell Death & Disease, 2021

Global dysregulation of RNA splicing and imbalanced sphingolipid metabolism has emerged as promot... more Global dysregulation of RNA splicing and imbalanced sphingolipid metabolism has emerged as promoters of cancer cell transformation. Here, we present specific signature of alternative splicing (AS) events of sphingolipid genes for each breast cancer subtype from the TCGA-BRCA dataset. We show that ceramide synthase 2 (CERS2) undergoes a unique cassette exon event specifically in Luminal B subtype tumors. We validated this exon 8 skipping event in Luminal B cancer cells compared to normal epithelial cells, and in patient-derived tumor tissues compared to matched normal tissues. Differential AS-based survival analysis shows that this AS event of CERS2 is a poor prognostic factor for Luminal B patients. As Exon 8 corresponds to catalytic Lag1p domain, overexpression of AS transcript of CERS2 in Luminal B cancer cells leads to a reduction in the level of very-long-chain ceramides compared to overexpression of protein-coding (PC) transcript of CERS2. We further demonstrate that this AS ev...

IETE Journal of Research, 2020

At present, Electrical Impedance Tomography (EIT) is a simplified, non-invasive, cost-effective, ... more At present, Electrical Impedance Tomography (EIT) is a simplified, non-invasive, cost-effective, vogue and standard technique to reckon the electrical properties of the interior of a biological tissue by the measurements of voltage and current signals from its boundary. The EIT system, consisting of 16 or 8 or 4 electrodes, is attached to the circumference of an object. A conventional EIT system consists of a precise current source that can generate a high-frequency, low-amplitude current signal (generally in order of mA) that is to be given in two electrodes of the electrode array which can be selected through fast multiplexer switching controlled by a controller. The current injected to these two electrodes produces the voltage drop to other remaining unconnected electrodes which can be measured in terms of induced voltages and this measurement cycle continues by changing the electrode. This designed module provides effective information in terms of relative voltage data to the Personal Computer (PC) equipped with the GUI based on MATLAB for the further analysis through a wired/wireless connection. This algorithm of all functions is related to image reconstruction, including the impedance plethysmography using a MatLab Graphical User Interface (GUI). Also, analysis part has been also done by using the same GUI to construct an image. Several simulation experiments have been performed to evaluate the performance of the proposed EIT system and algorithm. Thus, we conclude the proposed EIT system to improve for better image resolution. The designed EIT system would be useful in industrial and medical applications.

International Journal of Intelligent Systems and Applications in Engineering, 2018

This paper discussed the Design and implementation of the electrical impedance-based system, whic... more This paper discussed the Design and implementation of the electrical impedance-based system, which covers both techniques (Impedance Plethysmography (IPG) and Electrical Impedance Tomography (EIT)). The electrical impedance distribution image of the cross-section of a phantom based on current excitation and voltage measurement using electrodes pair is reconstructs image according to the electrical property of the medical phantom. The electrical impedance based image provides the significant morphological information. Image quality depends upon many other iterative process and image processing algorithms in addition to. The hardware designing of the system is a most important part of all impedance-based techniques. In measurement section, the current source of the electrical impedancebased system should supply multi-frequency signal for measurement because it provides more useful information about the phantom. Therefore, a signal source that provides accurate excitation and a reference signal for measurement is more useful. Matlab is used for image reconstruction and processing of the impedance data.

PLOS ONE, 2019

The approval of biosimilars requires demonstration of biosimilarity, which rests on the base of t... more The approval of biosimilars requires demonstration of biosimilarity, which rests on the base of thorough analytical characterization of the biosimilar product. In addition to demonstration of biosimilarity, the product related impurities need to be thoroughly characterized and controlled at minimal levels. Pegylation of peptides and proteins creates significant challenges for detailed structural characterization, such as PEG (Poly Ethylene Glycol) heterogeneity, site of addition and number of attached pegylated moieties. A combination of several methods including circular dichroism, FTIR (Fourier-transform Infrared Spectroscopy), fluorescence spectroscopy, DSC (Differential Scanning Calorimetry), 1D and 2D NMR (Nuclear Magnetic Resonance), Edman degradation and peptide mapping by LC-MS (Liquid Chromatography Mass Spectrometry) were used for characterization of N-terminally pegylated filgrastim. Product related impurities such as oxidized, reduced, deamidated, dipegylated variants and monopegylated positional isomers have been characterized in detail using various HPLC (High Performance Liquid Chromatography) based methods and LC-MS techniques. The functional characterization in terms of receptor binding and cell proliferation assay was conducted for the similarity assessment and the potential impact of the product variants on the in vitro biological activity has also been assessed. In summary, this study presents, for the first time, a detailed structural and molecular level characterization of a biosimilar pegfilgrastim providing a strong base for the demonstration of overall biosimilarity of the product with the innovator product.

IOSR Journal of Dental and Medical Sciences, 2017

Aims and objectives: We conducted this study to evaluate the role of glutamine supplemented total... more Aims and objectives: We conducted this study to evaluate the role of glutamine supplemented total parenteral nutrition (TPN) in severe acute pancreatitis. Methods: Forty patients with severe acute pancreatitis admitted at Rajendra institute of medical Sciences ,Ranchi, over a period of one year(July 2012 to June 2013) were randomly divided into two therapeutic groups. Patients in group 1(19 in no) and group 2 (21 in no.) were treated with standard TPN and glutamine supplemented TPN respectively. Patients were assessed for nutritional parameters, the incidence of complications, mortality, length of hospital stay(LOS) and length of TPN. Results: Majority of patients were male in both groups(60 % in group 1 vs. 62% in group 2) and the average age of presentation was also same(41.13+ 4.46 years in group 1 vs.39+3.96 years in group 2). Gall stone was the most common etiological factor followed by alcohol. The incidence of complications in group 1 was much higher(40.25%) than those in group 2 (30%). Mortalities for group 1 and 2 were 15.78 %(3/19) and 9.52%(2/21) respectively. The length of hospital stay in group 1(21.08+2.80days) was longer than those of group 2 (19.33+2.62 days). The length of TPN was alsolonger in group 1 (14.47+2.72 days) than those of group 2 (10.56+2.21 days). Conclusion: Glutamine supplemented TPN did not significantly decrease the mortality or rate of complications but it reduced the number of hospital days admission and duration of TPN. Further studies with large number of patients is needed in Indian setting.

Archives of International Surgery, 2016

Background: Due to the limited availability of literature on management of burns during pregnancy... more Background: Due to the limited availability of literature on management of burns during pregnancy, many surgical units face the difficult task of handling such cases. Keeping this in mind, a retrospective analysis of all the burnt females with pregnancy was done. Patients and Methods: A total of 19 cases of burns in pregnancy were analyzed in this study. The demographic characteristic and stage of pregnancy of the patients were evaluated. In addition, the causes, severity of the treatment, and outcome were noted. Results: The highest number of cases was in the 2nd trimester. The third trimester was associated with the highest incidence of abortion. Abortion was spontaneous in 70% of the cases. Maternal deaths were least in the 1st trimester. The rate of spontaneous abortion was highest in the 1st trimester. Conclusion: Burns in pregnancy are associated with high fetal loss. Prevention of burns in the pregnant woman is essential in reducing the morbidity and mortality associated with these injuries.