shastri taduri - Academia.edu (original) (raw)

Papers by shastri taduri

ABSTRACT Adenanthera pavonina L.(vernacular:Madatiya) is a medicinal plant, belongs to family Fab... more ABSTRACT Adenanthera pavonina L.(vernacular:Madatiya) is a medicinal plant, belongs to family Fabaceae. It is widely used for the treatments of many diseases in various therapeutic systems including Ayruveda therapeutic system. The medicinal uses and the distribution of the species were studied using a structured questionnaire survey considering 100 sample population. Morphological characters were analyzed using the specimens collected from their natural habitats to infer the phenetic relationships and has shown no variations with respect to their habitat differences. Qualitative phytochemical analysis was done to identify the chemical compounds present in the stem bark extracts of different solvents such as hexane, chloroform, methanol and water. Phytochemical screening of stem barks of the different samples confirms the presence of phytochemicals; alkaloids, flavonoids, glycosides, tannins, steroids, proteins and saponins in the extracts of the methanolic, chloroform and water. This study draws attention to the need of further analysis of the active principles of the species in order to understand their mode of action in controlling different diseases

Somaclonal variations were studied in in vitro regenerated plants of an endangered cucurbit, Citr... more Somaclonal variations were studied in in vitro regenerated plants of an endangered cucurbit, Citrullus colosynthis (L.) commonly known as ‘bitter apple’. It is a medicinally important plant used as a antirheumatic, anthelminthic and its exractive ‘colosynth’ is a very strong laxative. Roots are used in urinary diseases, mammalitis and opthalmia. Its exract cucurbitacin glucosides inhibit growth of human breast cancer. During in vitro mutagenesis and regeneration studies a number of somaclones have been isolated and characterized. Regenerated plants fromR0 are scored for the identification of variant plants in R1 and R2 generation. During these studies a number of variations in habit, leaf and tendrillar character, floral somaclones like androecious, gynoecious, andro monoecious and andro gynoecious were isolated. Variation in fruit number, sizes, colours, and seed coat colours were also screened.

Agronomy, 2019

Drought is a major abiotic stress factor affecting the growth and development of plants at all st... more Drought is a major abiotic stress factor affecting the growth and development of plants at all stages. Developing a screening tool for identifying drought stress tolerance during seedling establishment is important in the deployment of rice varieties suited to water-limited growing environments. An experiment was conducted to evaluate 100 rice genotypes, mostly belonging to the tropical japonica subspecies, for drought stress tolerance using low-cost, pre-fabricated mini-hoop structures. The rice seedlings were subjected to two different soil moisture regimes- control pots managed at 100% and drought pots at 50% field capacity, from 12 to 30 days after sowing (DAS). Several morpho-physiological parameters including root traits were measured to assess the response of genotypes to drought stress. Significant moisture stress × genotype interactions were found for most of the parameters measured. A cumulative drought stress response index (CDSRI) was developed by adding the individual r...

Journal of Plant Biotechnology, 2018

In the present study in vitro mutagenesis was used to study the effect of gamma irradiation and E... more In the present study in vitro mutagenesis was used to study the effect of gamma irradiation and EMS on callus induction, morphogenesis and production of multiple shoots from different explants of Citrullus colocynthis (L.) Schrad. Gamma radiations (5 kR to 20 kR) and certain chemicals have been effected on plant growth developments and changes of biochemical metabolisms in plants. Murashige and Skoog (MS) medium containing with auxins such as NAA, IAA, 2,4-D (0.5~2.0 mg/l), cytokinines BAP, kn TDZ, (0.5~2.5 mg/l), L-Glutamic acid (1~2 mg/l) and Coconut milk (10~20%). After 5 weeks on induction media, explants and callus (EC) were exposed to 5 kR, 10 kR, 15 kR and 20kR, of gamma radiation and treated with 1, 2, 3, 4 and 5 mM ethyl methane sulphonate (EMS) for 30 min. The highest percentage of callusing was observed (70%) stem irradiated with 5 kR and significantly decrease in fresh and dry weight of callus in the below 4 kR doses and above 20 kR doses, there was a progressive decrease in the fresh weight and dry weights when compared to control callus. Maximum percentage of plantlet regeneration (59%) was induced from callus exposed to 15 kR gamma irradiation on MS media fortified with 2.0 mg/l 2,4-D + 2.0 mg/l BAP + 2.0 mg/l L-glutamic acid. Increase in gamma irradiation dose above 15 kR and 5 mM EMS reduced regeneration capacity of callus. Doses higher than 20 kR and 7 mM EMS was lethal to micropropagated plants of Citurullus colocynthis.

Physiology and Molecular Biology of Plants, 2016

Asian Journal of Biotechnology, 2015

An efficient procedure has been developed for induction of adventitious rooting and proliferation... more An efficient procedure has been developed for induction of adventitious rooting and proliferation from different explants of Citrullus colocynthis. Leaf, stem, cotyledon and hypocotyledon explants cultured on different concentration of auxins and cytokinins, were supplemented on MS medium to test their ability to induce rhizogenesis. Murashige and Skoog (MS) medium fortified with 2,4-dichlorophenoxyacetic (2,4-D), indoleacetic acid (IAA), Indole-Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA). Each at the concentrations of 0, 0.5, 1.0, 1.5, 2.0 and 2.5 mg LG 1 were evaluated for their effects on adventitious root induction in the present study. High concentration of IAA (2.0 mg LG 1) and low concentration of IBA (1.5 mg LG 1) is best for induction of rhizogenesis. High concentration of IBA favored formation of malformed and thick roots. The NAA in combination with IBA is most potential in inducing high percentage of rooting (50%) and IAA in combination with IBA induced maximum rooting 62% with highest number of roots per shoot (3.80±0.2) and root length (3.8±0.1 cm) were obtained from leaf, cotyledon and stem than hypocotyls. Results revealed that, the best rooting ability was observed from in stem explants cultured on MS medium supplemented with 2.0 mg LG 1 IAA and 1.5 mg LG 1 IBA, multiple roots fibrous were produced from cotyledon explants on MS fortified with 2.0 mg LG 1 2,4-d and multiple roots cluster produced from leaf explants 1.5 mg LG 1 IBA and 2.0 mg LG 1 IAA. A distinct feature of this study is the adventitious rooting and proliferations from different explants of Citrullus colocynthis which has not been reported previously.

Current Trends in Biotechnology and Pharmacy

DESCRIPTION Shoot differentiation from seedling explants of Erythrina variegata, an important med... more DESCRIPTION Shoot differentiation from seedling explants of Erythrina variegata, an important medicinal plant, was tried on Murashige and Skoog’s (MS) medium containing different plant growth regulators, but without any success. Thus, Erythrina variegata appeared to be a highly recalcitrant leguminous plant. However, rhizogenesis was frequent and achieved directly from different seedling explants (stem, leaf and roots) as well as from explant derived calli. Roots were produced along with callus formation. NAA is the most effective auxin for induction of roots. Natural auxins IAA and IBA were also used for rooting. The media used were (1) MS + 2 mg/l 2,4-D + 1 mg/l NAA and (2) MS + 2 mg/l 2,4-D + 1 mg/l BAP + 1 mg/l IBA for in vitro induction of roots from root, stem and leaf calli respectively. Thus, like in other legumes, in the present study also, Erythrina displayed a strong ability for in vitro rhizogenesis than caulogenesis.

Callus culture is very useful to extract commercially important secondary metabolites. If a small... more Callus culture is very useful to extract commercially important secondary metabolites. If a small piece of tissue from medicinally important plant is cultured in vitro and produced callus culture, then secondary metabolites or drugs can be directly extracted from the callus tissue without destroying the complete plant. As a result, this method helps the conservation of medicinally important plants in nature. In the present study of Citrullus colocynthis, callus induction was attempted by culturing cotyledon, young leaf and stem. The maximum number of callus induction was achieved from stem explants on MS medium enriched with o.5 mg/l IAA and 1.0 mg/l 2,4-D and other combination of auxin and cytokinin MS medium with 2.0 mg/l BAP and 0.5mg/l NAA, few shoots and roots were obtained .

During the in vitro cultures of Trichosanthes anguina and T. kirlowii, effect of various concentr... more During the in vitro cultures of Trichosanthes anguina and T. kirlowii, effect of various concentrations of plant growth regulators (PGR’s) were studies on the production of a secondarymetabolite, “trichosanthin” (TCN) which is effective in inhibiting human immunodeficiency virus I replication in Lymphocytes and macrophase cells in vitro. It is a wonder drug used for curing AIDS[1] . NAA Kinetin TDZ and BAP attached the yield of trichosanthin (TCN). Identification of trichosanthin in each of the tissues was carried out by thin layer chromatography (TLC).

In vitro mutagenesis " is defined as the induction of mutation in cell cultures maintained i... more In vitro mutagenesis " is defined as the induction of mutation in cell cultures maintained in vitro by the use of chemical and physical mutagens and subsequent establishment of cell lines and regeneration of mutant plants. In the present paper, the effect of gamma rays was studied on morphogenesis, rooting efficiency, caulogenesis and multiple shoot production in Erythrina variegata L. Among the explants studied stem and petiole have responded well in induction of caulogenesis, rhyzogenesis and multiple shoots in 3-5 kR gamma irradiation.

Plant Cell Biotechnology and Molecular Biology

Efficient plant regeneration was established in Citrullus colocynthis (L.) Schard by using leaf e... more Efficient plant regeneration was established in Citrullus colocynthis (L.) Schard by using leaf explants. By showing effects of various plant growth regulators alone and combinations (BAP, TDZ, IAA, NAA and IBA) at different concentration. Maximum numbers of shoots were obtained on Murashige and Skoog media supplemented with 2.27 μM/L TDZ and 2.85 μM/L IAA at an average of 18.28±0.29 shoots per explants. Whereas leaf explants placed on MS media supplemented with only TDZ in different concentrations (2.27, 4.55, 6.83, 9.10, 11.37 and 13.65 μM/L) was able to produce friable callus only in the time of 4 to 5 weeks. Low level concentration of GA3 (1.87 μM/L) promoted efficient shoot elongation at an average shoot length of 9.9±0.17 cm. Elongated shoots were sub cultured on MS media containing IBA, IAA and NAA individually. 17.13 μM/L of IBA found to be optimum concentration of effective rhizogenesis, at average root length was 7.45±0.87 detected per shoot within the 2weeks after shiftin...

International Journal of Pharma and Bio Sciences

In the present investigation, the seeds of (Lycopersicon esculentum Mill.) variety Pusa Ruby were... more In the present investigation, the seeds of (Lycopersicon esculentum Mill.) variety Pusa Ruby were used to evaluate as to what effect this heavy metal mercury would create on the vegetable crop plant at different stages of its growth and development. The heavy metal mercury does and duration of treatment at optimal and suitable level was finalized after trial experiments conducted by considering present germination and seedling growth. At lower concentration treatment showed enhanced percentage of germination, survival percentage, plant height, root length, early flowering more pollen viability, increase in total chlorophyll content. Apart from this metal analysis was also studied. Different yield components such as number of fruits / plant, fruit weight and fruit girth were under taken. The higher concentration of heavy metal mercury treatments showed inhibitory effect in general. The results were discussed in relation to treatment and concentration of heavy metal mercury.

International Journal of Pharma and Bio Sciences

Efficient plant regeneration via organogenesis was established using leaf and stem explant. Callu... more Efficient plant regeneration via organogenesis was established using leaf and stem explant. Callus cultures from the leaf and stem explants were tested for growth and organogenic capacity on MS medium fortified with different concentrations and combination of 2,4-D with BAP and 2,4-D with TDZ. The maximum morphogenic callus induction rate (65%) was observed from leaf explant by culturing in MS medium supplemented with 1.5 mg/l 2,4-D + 1.0 mg/l BAP when compared to 2.0 mg/l 2,4-D + 1.0 mg/l TDZ (50%). High frequency shoot regeneration (75%) from leaf derived callus was observed on MS medium supplemented with 2.5 mg/l 2,4-D and 0.5 mg/l TDZ. At the end of 3 weeks the regenerated shoots were transferred on the same medium (MS + 2.5 mg/l 2,4-D + 0.5 mg/l TDZ) for further proliferation and elongation. The regenerated shoots were rooted with high frequency (60%) in MS medium supplemented with 1.5 mg/l IBA when compared to other auxin NAA. The in vitro raised plantlets were successfully es...

ABSTRACT We studied the effect of Chromium toxicity on growth, chlorophyll and yield attributes. ... more ABSTRACT We studied the effect of Chromium toxicity on growth, chlorophyll and yield attributes. Increasing concentrations of chromium caused reduction in growth, chlorophyll contents, percentage of seed germination, plant height, root length, flowering, pollen viability and yield. The potential of plants with the capacity to accumulate chromium compounds for bioremediation of cr contamination has gained interest in recent years. We conclude that high concentration of cr toxicity had strong inhibition and damage to the normal metabolism and cell implement structure, as well as on the function of tomato plant.

Journal of Herbs, Spices & Medicinal Plants, 2014

ABSTRACT Adenanthera pavonina L.(vernacular:Madatiya) is a medicinal plant, belongs to family Fab... more ABSTRACT Adenanthera pavonina L.(vernacular:Madatiya) is a medicinal plant, belongs to family Fabaceae. It is widely used for the treatments of many diseases in various therapeutic systems including Ayruveda therapeutic system. The medicinal uses and the distribution of the species were studied using a structured questionnaire survey considering 100 sample population. Morphological characters were analyzed using the specimens collected from their natural habitats to infer the phenetic relationships and has shown no variations with respect to their habitat differences. Qualitative phytochemical analysis was done to identify the chemical compounds present in the stem bark extracts of different solvents such as hexane, chloroform, methanol and water. Phytochemical screening of stem barks of the different samples confirms the presence of phytochemicals; alkaloids, flavonoids, glycosides, tannins, steroids, proteins and saponins in the extracts of the methanolic, chloroform and water. This study draws attention to the need of further analysis of the active principles of the species in order to understand their mode of action in controlling different diseases

Somaclonal variations were studied in in vitro regenerated plants of an endangered cucurbit, Citr... more Somaclonal variations were studied in in vitro regenerated plants of an endangered cucurbit, Citrullus colosynthis (L.) commonly known as ‘bitter apple’. It is a medicinally important plant used as a antirheumatic, anthelminthic and its exractive ‘colosynth’ is a very strong laxative. Roots are used in urinary diseases, mammalitis and opthalmia. Its exract cucurbitacin glucosides inhibit growth of human breast cancer. During in vitro mutagenesis and regeneration studies a number of somaclones have been isolated and characterized. Regenerated plants fromR0 are scored for the identification of variant plants in R1 and R2 generation. During these studies a number of variations in habit, leaf and tendrillar character, floral somaclones like androecious, gynoecious, andro monoecious and andro gynoecious were isolated. Variation in fruit number, sizes, colours, and seed coat colours were also screened.

Agronomy, 2019

Drought is a major abiotic stress factor affecting the growth and development of plants at all st... more Drought is a major abiotic stress factor affecting the growth and development of plants at all stages. Developing a screening tool for identifying drought stress tolerance during seedling establishment is important in the deployment of rice varieties suited to water-limited growing environments. An experiment was conducted to evaluate 100 rice genotypes, mostly belonging to the tropical japonica subspecies, for drought stress tolerance using low-cost, pre-fabricated mini-hoop structures. The rice seedlings were subjected to two different soil moisture regimes- control pots managed at 100% and drought pots at 50% field capacity, from 12 to 30 days after sowing (DAS). Several morpho-physiological parameters including root traits were measured to assess the response of genotypes to drought stress. Significant moisture stress × genotype interactions were found for most of the parameters measured. A cumulative drought stress response index (CDSRI) was developed by adding the individual r...

Journal of Plant Biotechnology, 2018

In the present study in vitro mutagenesis was used to study the effect of gamma irradiation and E... more In the present study in vitro mutagenesis was used to study the effect of gamma irradiation and EMS on callus induction, morphogenesis and production of multiple shoots from different explants of Citrullus colocynthis (L.) Schrad. Gamma radiations (5 kR to 20 kR) and certain chemicals have been effected on plant growth developments and changes of biochemical metabolisms in plants. Murashige and Skoog (MS) medium containing with auxins such as NAA, IAA, 2,4-D (0.5~2.0 mg/l), cytokinines BAP, kn TDZ, (0.5~2.5 mg/l), L-Glutamic acid (1~2 mg/l) and Coconut milk (10~20%). After 5 weeks on induction media, explants and callus (EC) were exposed to 5 kR, 10 kR, 15 kR and 20kR, of gamma radiation and treated with 1, 2, 3, 4 and 5 mM ethyl methane sulphonate (EMS) for 30 min. The highest percentage of callusing was observed (70%) stem irradiated with 5 kR and significantly decrease in fresh and dry weight of callus in the below 4 kR doses and above 20 kR doses, there was a progressive decrease in the fresh weight and dry weights when compared to control callus. Maximum percentage of plantlet regeneration (59%) was induced from callus exposed to 15 kR gamma irradiation on MS media fortified with 2.0 mg/l 2,4-D + 2.0 mg/l BAP + 2.0 mg/l L-glutamic acid. Increase in gamma irradiation dose above 15 kR and 5 mM EMS reduced regeneration capacity of callus. Doses higher than 20 kR and 7 mM EMS was lethal to micropropagated plants of Citurullus colocynthis.

Physiology and Molecular Biology of Plants, 2016

Asian Journal of Biotechnology, 2015

An efficient procedure has been developed for induction of adventitious rooting and proliferation... more An efficient procedure has been developed for induction of adventitious rooting and proliferation from different explants of Citrullus colocynthis. Leaf, stem, cotyledon and hypocotyledon explants cultured on different concentration of auxins and cytokinins, were supplemented on MS medium to test their ability to induce rhizogenesis. Murashige and Skoog (MS) medium fortified with 2,4-dichlorophenoxyacetic (2,4-D), indoleacetic acid (IAA), Indole-Butyric Acid (IBA) and Naphthalene Acetic Acid (NAA). Each at the concentrations of 0, 0.5, 1.0, 1.5, 2.0 and 2.5 mg LG 1 were evaluated for their effects on adventitious root induction in the present study. High concentration of IAA (2.0 mg LG 1) and low concentration of IBA (1.5 mg LG 1) is best for induction of rhizogenesis. High concentration of IBA favored formation of malformed and thick roots. The NAA in combination with IBA is most potential in inducing high percentage of rooting (50%) and IAA in combination with IBA induced maximum rooting 62% with highest number of roots per shoot (3.80±0.2) and root length (3.8±0.1 cm) were obtained from leaf, cotyledon and stem than hypocotyls. Results revealed that, the best rooting ability was observed from in stem explants cultured on MS medium supplemented with 2.0 mg LG 1 IAA and 1.5 mg LG 1 IBA, multiple roots fibrous were produced from cotyledon explants on MS fortified with 2.0 mg LG 1 2,4-d and multiple roots cluster produced from leaf explants 1.5 mg LG 1 IBA and 2.0 mg LG 1 IAA. A distinct feature of this study is the adventitious rooting and proliferations from different explants of Citrullus colocynthis which has not been reported previously.

Current Trends in Biotechnology and Pharmacy

DESCRIPTION Shoot differentiation from seedling explants of Erythrina variegata, an important med... more DESCRIPTION Shoot differentiation from seedling explants of Erythrina variegata, an important medicinal plant, was tried on Murashige and Skoog’s (MS) medium containing different plant growth regulators, but without any success. Thus, Erythrina variegata appeared to be a highly recalcitrant leguminous plant. However, rhizogenesis was frequent and achieved directly from different seedling explants (stem, leaf and roots) as well as from explant derived calli. Roots were produced along with callus formation. NAA is the most effective auxin for induction of roots. Natural auxins IAA and IBA were also used for rooting. The media used were (1) MS + 2 mg/l 2,4-D + 1 mg/l NAA and (2) MS + 2 mg/l 2,4-D + 1 mg/l BAP + 1 mg/l IBA for in vitro induction of roots from root, stem and leaf calli respectively. Thus, like in other legumes, in the present study also, Erythrina displayed a strong ability for in vitro rhizogenesis than caulogenesis.

Callus culture is very useful to extract commercially important secondary metabolites. If a small... more Callus culture is very useful to extract commercially important secondary metabolites. If a small piece of tissue from medicinally important plant is cultured in vitro and produced callus culture, then secondary metabolites or drugs can be directly extracted from the callus tissue without destroying the complete plant. As a result, this method helps the conservation of medicinally important plants in nature. In the present study of Citrullus colocynthis, callus induction was attempted by culturing cotyledon, young leaf and stem. The maximum number of callus induction was achieved from stem explants on MS medium enriched with o.5 mg/l IAA and 1.0 mg/l 2,4-D and other combination of auxin and cytokinin MS medium with 2.0 mg/l BAP and 0.5mg/l NAA, few shoots and roots were obtained .

During the in vitro cultures of Trichosanthes anguina and T. kirlowii, effect of various concentr... more During the in vitro cultures of Trichosanthes anguina and T. kirlowii, effect of various concentrations of plant growth regulators (PGR’s) were studies on the production of a secondarymetabolite, “trichosanthin” (TCN) which is effective in inhibiting human immunodeficiency virus I replication in Lymphocytes and macrophase cells in vitro. It is a wonder drug used for curing AIDS[1] . NAA Kinetin TDZ and BAP attached the yield of trichosanthin (TCN). Identification of trichosanthin in each of the tissues was carried out by thin layer chromatography (TLC).

In vitro mutagenesis " is defined as the induction of mutation in cell cultures maintained i... more In vitro mutagenesis " is defined as the induction of mutation in cell cultures maintained in vitro by the use of chemical and physical mutagens and subsequent establishment of cell lines and regeneration of mutant plants. In the present paper, the effect of gamma rays was studied on morphogenesis, rooting efficiency, caulogenesis and multiple shoot production in Erythrina variegata L. Among the explants studied stem and petiole have responded well in induction of caulogenesis, rhyzogenesis and multiple shoots in 3-5 kR gamma irradiation.

Plant Cell Biotechnology and Molecular Biology

Efficient plant regeneration was established in Citrullus colocynthis (L.) Schard by using leaf e... more Efficient plant regeneration was established in Citrullus colocynthis (L.) Schard by using leaf explants. By showing effects of various plant growth regulators alone and combinations (BAP, TDZ, IAA, NAA and IBA) at different concentration. Maximum numbers of shoots were obtained on Murashige and Skoog media supplemented with 2.27 μM/L TDZ and 2.85 μM/L IAA at an average of 18.28±0.29 shoots per explants. Whereas leaf explants placed on MS media supplemented with only TDZ in different concentrations (2.27, 4.55, 6.83, 9.10, 11.37 and 13.65 μM/L) was able to produce friable callus only in the time of 4 to 5 weeks. Low level concentration of GA3 (1.87 μM/L) promoted efficient shoot elongation at an average shoot length of 9.9±0.17 cm. Elongated shoots were sub cultured on MS media containing IBA, IAA and NAA individually. 17.13 μM/L of IBA found to be optimum concentration of effective rhizogenesis, at average root length was 7.45±0.87 detected per shoot within the 2weeks after shiftin...

International Journal of Pharma and Bio Sciences

In the present investigation, the seeds of (Lycopersicon esculentum Mill.) variety Pusa Ruby were... more In the present investigation, the seeds of (Lycopersicon esculentum Mill.) variety Pusa Ruby were used to evaluate as to what effect this heavy metal mercury would create on the vegetable crop plant at different stages of its growth and development. The heavy metal mercury does and duration of treatment at optimal and suitable level was finalized after trial experiments conducted by considering present germination and seedling growth. At lower concentration treatment showed enhanced percentage of germination, survival percentage, plant height, root length, early flowering more pollen viability, increase in total chlorophyll content. Apart from this metal analysis was also studied. Different yield components such as number of fruits / plant, fruit weight and fruit girth were under taken. The higher concentration of heavy metal mercury treatments showed inhibitory effect in general. The results were discussed in relation to treatment and concentration of heavy metal mercury.

International Journal of Pharma and Bio Sciences

Efficient plant regeneration via organogenesis was established using leaf and stem explant. Callu... more Efficient plant regeneration via organogenesis was established using leaf and stem explant. Callus cultures from the leaf and stem explants were tested for growth and organogenic capacity on MS medium fortified with different concentrations and combination of 2,4-D with BAP and 2,4-D with TDZ. The maximum morphogenic callus induction rate (65%) was observed from leaf explant by culturing in MS medium supplemented with 1.5 mg/l 2,4-D + 1.0 mg/l BAP when compared to 2.0 mg/l 2,4-D + 1.0 mg/l TDZ (50%). High frequency shoot regeneration (75%) from leaf derived callus was observed on MS medium supplemented with 2.5 mg/l 2,4-D and 0.5 mg/l TDZ. At the end of 3 weeks the regenerated shoots were transferred on the same medium (MS + 2.5 mg/l 2,4-D + 0.5 mg/l TDZ) for further proliferation and elongation. The regenerated shoots were rooted with high frequency (60%) in MS medium supplemented with 1.5 mg/l IBA when compared to other auxin NAA. The in vitro raised plantlets were successfully es...

ABSTRACT We studied the effect of Chromium toxicity on growth, chlorophyll and yield attributes. ... more ABSTRACT We studied the effect of Chromium toxicity on growth, chlorophyll and yield attributes. Increasing concentrations of chromium caused reduction in growth, chlorophyll contents, percentage of seed germination, plant height, root length, flowering, pollen viability and yield. The potential of plants with the capacity to accumulate chromium compounds for bioremediation of cr contamination has gained interest in recent years. We conclude that high concentration of cr toxicity had strong inhibition and damage to the normal metabolism and cell implement structure, as well as on the function of tomato plant.

Journal of Herbs, Spices & Medicinal Plants, 2014