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Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2019
In this colorimetric assay for sensitive detection of prostate specific antigen (PSA) tumor marke... more In this colorimetric assay for sensitive detection of prostate specific antigen (PSA) tumor marker, adsorbed non-thiolated poly-Adenine aptamer (polyA Apt) on the gold nanoparticles (AuNPs) surface was used. By incubating the AuNPs and the PSA specific aptamer prior to target addition, polyA Apt adsorbed on the gold nanoparticles and could bind the target while preventing non-specific interactions. Adsorbed polyA Apt on the AuNPs prevents aggregation of them by poly(diallyldimethylammoniumchloride) (PDDA). Upon the addition of PSA, it bind to the polyA Apt and induce the formation of a secondary structure. Therefore, interaction between polyA Apt and PDDA is repressed and PDDA induce the aggregation of the AuNPs. This analytical platform produces a remarkable optical signal in the absence and presence of PSA that accompanied by a color change from red to blue. This effect as a sensing strategy can be observed with naked eyes and quantified by colorimetry via measurement of the ratio of absorbances at 680 nm and 520 nm. Fabricated aptasensor for detection of PSA is linear in the concentration range of 0.1e100 ng/ml with 20 pg/ml as the limit of detection (S/N ¼ 3). Because of the selectively recognized for PSA in the presence of other interfering substances, this proposed assay applied to real samples for the rapid screening of PSA.
Microchimica Acta, 2019
Two kinds of aptasensors for ampicillin (AMP) are described. The assay strategies include the use... more Two kinds of aptasensors for ampicillin (AMP) are described. The assay strategies include the use of gold nanoparticles (AuNPs) that were modified with (a) a thiolated aptamer (T-Apt), and (b) a non-thiolated polyadenine aptamer (polyA Apt). The AuNPs and the aptamers were brought to interaction prior to addition of AMP. T-Apt and polyA Apt are adsorbed on the AuNPs by different mechanisms. The adsorbed aptamer was able to bind the target while preventing non-specific interactions. Remarkably different optical absorbances (measured at 520 and 680 nm) are produced the absence and presence of AMP. The assay can selectively recognize AMP even in the presence of species of similar chemical structure. The T-Apt based assay has a linear response in the 1-600 nM AMP concentration range and a 0.1 nM limit of detection. The respective data for the polyA Apt assay are 1-400 nM and 0.49 nM.
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2019
In this colorimetric assay for sensitive detection of prostate specific antigen (PSA) tumor marke... more In this colorimetric assay for sensitive detection of prostate specific antigen (PSA) tumor marker, adsorbed non-thiolated poly-Adenine aptamer (polyA Apt) on the gold nanoparticles (AuNPs) surface was used. By incubating the AuNPs and the PSA specific aptamer prior to target addition, polyA Apt adsorbed on the gold nanoparticles and could bind the target while preventing non-specific interactions. Adsorbed polyA Apt on the AuNPs prevents aggregation of them by poly(diallyldimethylammoniumchloride) (PDDA). Upon the addition of PSA, it bind to the polyA Apt and induce the formation of a secondary structure. Therefore, interaction between polyA Apt and PDDA is repressed and PDDA induce the aggregation of the AuNPs. This analytical platform produces a remarkable optical signal in the absence and presence of PSA that accompanied by a color change from red to blue. This effect as a sensing strategy can be observed with naked eyes and quantified by colorimetry via measurement of the ratio of absorbances at 680 nm and 520 nm. Fabricated aptasensor for detection of PSA is linear in the concentration range of 0.1e100 ng/ml with 20 pg/ml as the limit of detection (S/N ¼ 3). Because of the selectively recognized for PSA in the presence of other interfering substances, this proposed assay applied to real samples for the rapid screening of PSA.
Microchimica Acta, 2019
Two kinds of aptasensors for ampicillin (AMP) are described. The assay strategies include the use... more Two kinds of aptasensors for ampicillin (AMP) are described. The assay strategies include the use of gold nanoparticles (AuNPs) that were modified with (a) a thiolated aptamer (T-Apt), and (b) a non-thiolated polyadenine aptamer (polyA Apt). The AuNPs and the aptamers were brought to interaction prior to addition of AMP. T-Apt and polyA Apt are adsorbed on the AuNPs by different mechanisms. The adsorbed aptamer was able to bind the target while preventing non-specific interactions. Remarkably different optical absorbances (measured at 520 and 680 nm) are produced the absence and presence of AMP. The assay can selectively recognize AMP even in the presence of species of similar chemical structure. The T-Apt based assay has a linear response in the 1-600 nM AMP concentration range and a 0.1 nM limit of detection. The respective data for the polyA Apt assay are 1-400 nM and 0.49 nM.