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Papers by sheeba afreen

Research paper thumbnail of Association of the circulating micro-RNAs with susceptible and newly diagnosed type 2 diabetes mellitus cases

Advances in Biomarker Sciences and Technology

Research paper thumbnail of A Comprehensive Review on the Role of Cell Growth and Apoptotic Genes Towards the Progression of CML

Era's Journal of Medical Research, 2019

Imatinib is a tyrosin kinase inhibitor, which has proven to be very effective in achieving high r... more Imatinib is a tyrosin kinase inhibitor, which has proven to be very effective in achieving high remission rates and improving prediction of treatment outcome. However a fraction of CML patients presents with resistance to this drug. Imatinib-based therapies were discontinued due to resistance and intolerance/noncompliance. the resistance for imatinib is developed due to the occurrence of point mutations in the BCR-ABL KD(kinase domain), although BCR-ABL amplification and impaired signaling pathways may contribute to the resistance. Other possible BCR-ABL independent mechanisms that influence resistance include reduced bioavailability of imatinib within Ph-positive cells, cytogenetic changes, disfunctionality of p53, and activation of alternative signaling pathways that promote cell survival and proliferation and reduced apoptosis. The achievement of Chronic myeloid leukemia (CML) is one of the most common leukemia. CML is described by a balanced genetic translocation, t(9;22)(q34;q11.2), in which the Abelson gene (ABL1) from chromosome 9q34 fused with the breakpoint cluster region (BCR) gene on chromosome 22q11.2. This genetic translocation is known as the Philadelphia chromosome. The molecular consequence of this translocation is the generation of a BCR-ABL1 fusion oncogene, which in turn translates into a BCR-ABL1 oncoprotein , which is the type of protein called a tyrosine kinase. This protein causes CML cells to grow and divide out of control. In a very small number of CML cases, the leukemia cells have the BCR-ABL oncogene but not the Philadelphia chromosome. It's thought that the BCR-ABL gene must form in a different way in these people. In an even smaller number of people who seem to have CML, neither the Philadelphia chromosome nor the BCR-ABL oncogene can be found. They might have other, unknown oncogenes causing their disease and are not considered to truly have CML.

Research paper thumbnail of Diagnostic Importance of Mirna in Diabetic Retinopathy

Era's Journal of Medical Research, 2019

Diabetes type II is an autoimmune disorder of multiple etiologies that is basically due to deregu... more Diabetes type II is an autoimmune disorder of multiple etiologies that is basically due to deregulation of blood glucose homeostatis, thus causing hyperglycemia accompanied by various other disturbances of carbohydrates, fat and protein metabolism. Broad classification of these diabetic complications involves two major groups-microvascular which includes retinopathy (leading to blindness), nephropathy (leading to renal failure) and neuropathy (leading to nerve damage) and macrovascular, including cardiovascular complexity and peripheral vascular disease. Diabetic retinopathy (DR) is a microangiopathy that induces optical complications in a diabetic subject that leads to acquired blindness in young adults due to high susceptibility of cellular components of retina towards the hyperglycemic environment. A large number of protein coding genes and some non-coding RNAs (ncRNAs) are reported to be involved, among which miRNAs are a small group of ncRNAs that are broadly studied to understand the pathology of the disease. Therefore, the functions and initiation of miRNAs could be regulated as their variation is allied with a broad array of functional defects, including incurable conditions. This review focuses on the molecular mechanisms of miRNA in response to DR.

Research paper thumbnail of Collection, Processing, Cryopresevation and Quantification of CD34+ Stem Cells in Umbilical Cord Blood

Research paper thumbnail of Evaluation of procedures for quantification of CD34 + haematopoietic stem cells and viability studies in umbilical cord blood derived mononuclear cells

Umbilical cord blood (UCB) transplantation is being used as an alternative source of haematopoiet... more Umbilical cord blood (UCB) transplantation is being used as an alternative source of haematopoietic stem cells for bone marrow reconstitution. Umbilical cord blood (UCB) transplantation is being used as an alternative source of haematopoietic stem cells for bone marrow reconstitution. Separation and processing of UCB samples in large numbers for storage in cord blood banks ideally needs to be partially automated. This study examines the loss of CD34 + cells concentration and viability of mononuclear cells from baseline verses one month, baseline verses six month and one month verses six month as well as standardization of procedure for cryopresevation of cord blood derived haematopoietic stem cells. A total of 500 umbilical cord blood units were collected. Samples were analyzed for CD34 + cells concentration and viability of mononuclear cells at baseline, one month and six month. In present study we found that the mean CD34 + cell loss from base line to one month was found to be 0.24℅, baseline to six month was 0.78℅ and one month to six month was 0.54℅. The mean viability of mononuclear cell loss from base line to one month was found to be 8.26℅, baseline to six month was 74.20℅ and one month to six month was8.99℅. Our data indicate that CD34 + cells and viability of mononuclear cells in UCB were maintained after cryopreservation of six month period in-80 0 C. This amount of CD34 + cells were within the range used for successful engraftment in both related and unrelated cord blood transplantation.

Research paper thumbnail of Evaluation of clinical outcome of cancer related thrombophilia: A Review

Biomedical Research

ABSTRACT

Research paper thumbnail of Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution

International Journal of Blood Transfusion and Immunohematology, 2011

Aims: Platelets are small, anucleated cytoplasmic fragments that play an essential role in blood ... more Aims: Platelets are small, anucleated cytoplasmic fragments that play an essential role in blood clotting and wound healing. Platelets are routinely stored in plasma for five days at 22°C. Aim: Our objective was to assess platelet morphology and function in random donor platelet stored for seven days in platelet additive solution. Methods: The random donor platelet was prepared by PRP method. Platelet concentrates from 50 blood donor were stored in 100% plasma (n=24) and 20:80 (n=24) additive solution. The RDP were placed in incubator and agitator at 22°C. Results: Out of total 50 samples, 48 samples were analyzed and two were discarded due to the bacterial contamination. On comparing the mean values of platelet count and MPV in both the groups with and without platelet additive solution, no significant difference was observed on day seven of storage period. A significant difference in PDW was observed on day seven (p < 0.001) in plasma. The mean values of LDH and pH showed no si...

Research paper thumbnail of Mechanism and pathophysiology of activated protein C-related factor V leiden in venous thrombosis

Asian journal of transfusion science, 2012

administered four times a day for seven postoperative days. [2] Histological analysis reported a ... more administered four times a day for seven postoperative days. [2] Histological analysis reported a pyogenic granuloma [Figure 1]. As far as we know, no consensus exists on the therapeutic management of AvWS in relation to oral soft tissues surgery. [2,3] The unusual dyscrasia reported here highlights that some AvWS patients may be unresponsive to DDAVP. Thus, the replacement therapy with factor VIII/VWF concentrates remains, the sole option of treatment in AvWS patients with IgM-MGUS, although this will produce transitory effects [4] as was the case in our patient. The photochemistry affinity of the diode lasers to hemoglobin can further help to achieve clot stabilization, by promoting the formation of induced sclerosis laser on the vascular network of the chorion [Figure 2]. Dentists may have a role in secondary prevention through the detection of latent AvWS, because they are able to manage minor bleeding problems-often encountered in their daily practiceduring oral surgery among dental patients.

Research paper thumbnail of Correlation of Birth Weight of Full Term Infants with Cord Blood derived CD34+ Cells Concentration

Journal of Tropical Pediatrics, 2011

Birth Weight of Full Term Infants with Cord Blood derived CD34 þ Cells Concentration Umbilical co... more Birth Weight of Full Term Infants with Cord Blood derived CD34 þ Cells Concentration Umbilical cord blood contains haematopoitic stem/ progenitor cells that have proven useful clinically to reconstitute the haematopoitic system in children and some adults. High cord blood nucleated and CD34 þ cell dose as well as the number of haematopoitic progenitor cells (Colony forming cells) in the transplant are associated with good transplantation results [1-4]. A number of factors such as maternal age, gestational age, newborn sex and weight, umbilical cord length, placental weight can influence volume, amount of mononuclear cell and CD34 þ cells. It has been shown that factors like newborn weight and sex as well as maternal age can affect the concentration of CD34 þ cells [5]. The aim of the present study is to find the relationship of birth weight of the baby for selection of cord blood unit donor so that sufficient mononuclear cell count and CD34 þ count could be obtained. This would help in reducing the costs for collection, transfer, storage and analysis of Cord Blood Units as well as select suitable donors for transplantation. About 500 umbilical cord blood samples were obtained from both vaginal and cesarean deliveries from the Department of Obstetrics and Gynecology, CSMMU, Lucknow. Cord blood was collected in anticoagulant citrate phosphate dextrose adenine (CPDA) triple blood bag. Processing was done in the Department of Transfusion Medicine, CSMMU, Lucknow. It was processed and mononuclear cells were isolated from it. The Mononuclear cell count was performed by automated cell counter (Sysmex KX-21, Japan). The viability was tested with Trypan Blue; dye exclusion method. The CD34 þ cells count was performed by using flow cytometery analysis. The Pearson correlation test was used for analysis. The level of statistically significance was set at 0.01 two sided for Pearson correlation (p < 0.01). Total 500 UCB samples were analyzed, concerning newborn sex distribution, 56.4% infants were males and 43.6% infants were females. The median gestational age was 38 weeks (mean 37.57 AE 1.82

Research paper thumbnail of Does Umbilical Cord Blood-derived CD34+ Cell Concentration Depend on the Weight and Sex of a Full-term Infant?

Journal of Pediatric Hematology/Oncology, 2012

Umbilical cord blood has recently been considered as an alternative source of hematopoietic proge... more Umbilical cord blood has recently been considered as an alternative source of hematopoietic progenitor cells for clinical application. Patient survival in allogenic cord blood transplantation is critically dependent on the cord blood-derived total nucleated cell count and the total CD34 cell count/kg of the body weight. A number of factors such as maternal age, gestational age, newborn&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s sex and weight, umbilical cord length, and placental weight can influence the volume, amount of mononuclear cells, and the CD34 cell concentration. Cord blood was collected from normal vaginal and cesarean deliveries. It was immediately processed and assessed for the total nucleated cell count and CD34 cell concentration. Assessment of maternal and neonatal parameters such as gestational age, baby&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s birth weight, and sex was carried out with the CD34 cell concentration. The mean CD34 cell concentration was 0.21±0.24% for the group with…

Research paper thumbnail of Correlation of umbilical cord blood volume with CD34+ cells concentration

International Journal of Blood Transfusion and Immunohematology, 2011

Aims: Umbilical cord blood (UCB) has been recently considered as an alternative source of hematop... more Aims: Umbilical cord blood (UCB) has been recently considered as an alternative source of hematopoitic progenitor cells for clinical application. The parameters commonly used to evaluate a UCB unit and predict transplant outcomes have been total nucleated cell count (TNCs), CD34+ cells concentration and total volume of cord blood collected. The volume of cord blood collection is also important for the high yield of CD34+ cells concentration and TNCs. The aim of the study was to find the correlation of umbilical cord blood volume with cord blood derived CD34+ cells concentration. Methods: Umbilical cord blood was collected from normal vaginal and cesarean deliveries. Total volume of cord blood collection was noted. It was immediately processed and assessed for total nucleated cells count and CD34+ cells concentration. Assessment of maternal and neonatal parameters such as mode of delivery, baby's birth weight and sex, cord blood volume and CD34+ cells concentration was made. Resu...

Research paper thumbnail of Role of miRNAs in cervical cancer: A comprehensive novel approach from pathogenesis to therapy

Journal of Gynecology Obstetrics and Human Reproduction, 2021

Human papillomaviruses (HPV) infection is a major causative agent and strongly associated with th... more Human papillomaviruses (HPV) infection is a major causative agent and strongly associated with the development of cervical cancer. Understanding the mechanisms of HPV-induced cervical cancer is extremely useful in therapeutic strategies for primary prevention (HPV vaccines) and secondary prevention (screening and diagnosis of precancerous lesions). However, due to the lack of proper implementation of screening programs in developing countries, cervical cancer is usually diagnosed at advanced stages that result in poor treatment responses. Nearly half of the patients will experience disease recurrence within two years post treatment. Therefore, it is vital to identify new tools for early diagnosis, prognosis, and treatment prediction. MicroRNAs (miRNAs) are small non-coding RNAs, implicated in posttranscriptional regulation of gene expression. Growing evidence has shown that abnormal miRNA expression is associated with cervical cancer progression, metastasis, and influences treatment outcomes. In this review, we provide comprehensive information about miRNA and their potential utility in cervical cancer diagnosis, prognosis, and clinical management to improve patient outcomes.

Research paper thumbnail of Association of the circulating micro-RNAs with susceptible and newly diagnosed type 2 diabetes mellitus cases

Advances in Biomarker Sciences and Technology

Research paper thumbnail of A Comprehensive Review on the Role of Cell Growth and Apoptotic Genes Towards the Progression of CML

Era's Journal of Medical Research, 2019

Imatinib is a tyrosin kinase inhibitor, which has proven to be very effective in achieving high r... more Imatinib is a tyrosin kinase inhibitor, which has proven to be very effective in achieving high remission rates and improving prediction of treatment outcome. However a fraction of CML patients presents with resistance to this drug. Imatinib-based therapies were discontinued due to resistance and intolerance/noncompliance. the resistance for imatinib is developed due to the occurrence of point mutations in the BCR-ABL KD(kinase domain), although BCR-ABL amplification and impaired signaling pathways may contribute to the resistance. Other possible BCR-ABL independent mechanisms that influence resistance include reduced bioavailability of imatinib within Ph-positive cells, cytogenetic changes, disfunctionality of p53, and activation of alternative signaling pathways that promote cell survival and proliferation and reduced apoptosis. The achievement of Chronic myeloid leukemia (CML) is one of the most common leukemia. CML is described by a balanced genetic translocation, t(9;22)(q34;q11.2), in which the Abelson gene (ABL1) from chromosome 9q34 fused with the breakpoint cluster region (BCR) gene on chromosome 22q11.2. This genetic translocation is known as the Philadelphia chromosome. The molecular consequence of this translocation is the generation of a BCR-ABL1 fusion oncogene, which in turn translates into a BCR-ABL1 oncoprotein , which is the type of protein called a tyrosine kinase. This protein causes CML cells to grow and divide out of control. In a very small number of CML cases, the leukemia cells have the BCR-ABL oncogene but not the Philadelphia chromosome. It's thought that the BCR-ABL gene must form in a different way in these people. In an even smaller number of people who seem to have CML, neither the Philadelphia chromosome nor the BCR-ABL oncogene can be found. They might have other, unknown oncogenes causing their disease and are not considered to truly have CML.

Research paper thumbnail of Diagnostic Importance of Mirna in Diabetic Retinopathy

Era's Journal of Medical Research, 2019

Diabetes type II is an autoimmune disorder of multiple etiologies that is basically due to deregu... more Diabetes type II is an autoimmune disorder of multiple etiologies that is basically due to deregulation of blood glucose homeostatis, thus causing hyperglycemia accompanied by various other disturbances of carbohydrates, fat and protein metabolism. Broad classification of these diabetic complications involves two major groups-microvascular which includes retinopathy (leading to blindness), nephropathy (leading to renal failure) and neuropathy (leading to nerve damage) and macrovascular, including cardiovascular complexity and peripheral vascular disease. Diabetic retinopathy (DR) is a microangiopathy that induces optical complications in a diabetic subject that leads to acquired blindness in young adults due to high susceptibility of cellular components of retina towards the hyperglycemic environment. A large number of protein coding genes and some non-coding RNAs (ncRNAs) are reported to be involved, among which miRNAs are a small group of ncRNAs that are broadly studied to understand the pathology of the disease. Therefore, the functions and initiation of miRNAs could be regulated as their variation is allied with a broad array of functional defects, including incurable conditions. This review focuses on the molecular mechanisms of miRNA in response to DR.

Research paper thumbnail of Collection, Processing, Cryopresevation and Quantification of CD34+ Stem Cells in Umbilical Cord Blood

Research paper thumbnail of Evaluation of procedures for quantification of CD34 + haematopoietic stem cells and viability studies in umbilical cord blood derived mononuclear cells

Umbilical cord blood (UCB) transplantation is being used as an alternative source of haematopoiet... more Umbilical cord blood (UCB) transplantation is being used as an alternative source of haematopoietic stem cells for bone marrow reconstitution. Umbilical cord blood (UCB) transplantation is being used as an alternative source of haematopoietic stem cells for bone marrow reconstitution. Separation and processing of UCB samples in large numbers for storage in cord blood banks ideally needs to be partially automated. This study examines the loss of CD34 + cells concentration and viability of mononuclear cells from baseline verses one month, baseline verses six month and one month verses six month as well as standardization of procedure for cryopresevation of cord blood derived haematopoietic stem cells. A total of 500 umbilical cord blood units were collected. Samples were analyzed for CD34 + cells concentration and viability of mononuclear cells at baseline, one month and six month. In present study we found that the mean CD34 + cell loss from base line to one month was found to be 0.24℅, baseline to six month was 0.78℅ and one month to six month was 0.54℅. The mean viability of mononuclear cell loss from base line to one month was found to be 8.26℅, baseline to six month was 74.20℅ and one month to six month was8.99℅. Our data indicate that CD34 + cells and viability of mononuclear cells in UCB were maintained after cryopreservation of six month period in-80 0 C. This amount of CD34 + cells were within the range used for successful engraftment in both related and unrelated cord blood transplantation.

Research paper thumbnail of Evaluation of clinical outcome of cancer related thrombophilia: A Review

Biomedical Research

ABSTRACT

Research paper thumbnail of Morphological and functional changes in random donor platelets stored for seven days in platelet additive solution

International Journal of Blood Transfusion and Immunohematology, 2011

Aims: Platelets are small, anucleated cytoplasmic fragments that play an essential role in blood ... more Aims: Platelets are small, anucleated cytoplasmic fragments that play an essential role in blood clotting and wound healing. Platelets are routinely stored in plasma for five days at 22°C. Aim: Our objective was to assess platelet morphology and function in random donor platelet stored for seven days in platelet additive solution. Methods: The random donor platelet was prepared by PRP method. Platelet concentrates from 50 blood donor were stored in 100% plasma (n=24) and 20:80 (n=24) additive solution. The RDP were placed in incubator and agitator at 22°C. Results: Out of total 50 samples, 48 samples were analyzed and two were discarded due to the bacterial contamination. On comparing the mean values of platelet count and MPV in both the groups with and without platelet additive solution, no significant difference was observed on day seven of storage period. A significant difference in PDW was observed on day seven (p < 0.001) in plasma. The mean values of LDH and pH showed no si...

Research paper thumbnail of Mechanism and pathophysiology of activated protein C-related factor V leiden in venous thrombosis

Asian journal of transfusion science, 2012

administered four times a day for seven postoperative days. [2] Histological analysis reported a ... more administered four times a day for seven postoperative days. [2] Histological analysis reported a pyogenic granuloma [Figure 1]. As far as we know, no consensus exists on the therapeutic management of AvWS in relation to oral soft tissues surgery. [2,3] The unusual dyscrasia reported here highlights that some AvWS patients may be unresponsive to DDAVP. Thus, the replacement therapy with factor VIII/VWF concentrates remains, the sole option of treatment in AvWS patients with IgM-MGUS, although this will produce transitory effects [4] as was the case in our patient. The photochemistry affinity of the diode lasers to hemoglobin can further help to achieve clot stabilization, by promoting the formation of induced sclerosis laser on the vascular network of the chorion [Figure 2]. Dentists may have a role in secondary prevention through the detection of latent AvWS, because they are able to manage minor bleeding problems-often encountered in their daily practiceduring oral surgery among dental patients.

Research paper thumbnail of Correlation of Birth Weight of Full Term Infants with Cord Blood derived CD34+ Cells Concentration

Journal of Tropical Pediatrics, 2011

Birth Weight of Full Term Infants with Cord Blood derived CD34 þ Cells Concentration Umbilical co... more Birth Weight of Full Term Infants with Cord Blood derived CD34 þ Cells Concentration Umbilical cord blood contains haematopoitic stem/ progenitor cells that have proven useful clinically to reconstitute the haematopoitic system in children and some adults. High cord blood nucleated and CD34 þ cell dose as well as the number of haematopoitic progenitor cells (Colony forming cells) in the transplant are associated with good transplantation results [1-4]. A number of factors such as maternal age, gestational age, newborn sex and weight, umbilical cord length, placental weight can influence volume, amount of mononuclear cell and CD34 þ cells. It has been shown that factors like newborn weight and sex as well as maternal age can affect the concentration of CD34 þ cells [5]. The aim of the present study is to find the relationship of birth weight of the baby for selection of cord blood unit donor so that sufficient mononuclear cell count and CD34 þ count could be obtained. This would help in reducing the costs for collection, transfer, storage and analysis of Cord Blood Units as well as select suitable donors for transplantation. About 500 umbilical cord blood samples were obtained from both vaginal and cesarean deliveries from the Department of Obstetrics and Gynecology, CSMMU, Lucknow. Cord blood was collected in anticoagulant citrate phosphate dextrose adenine (CPDA) triple blood bag. Processing was done in the Department of Transfusion Medicine, CSMMU, Lucknow. It was processed and mononuclear cells were isolated from it. The Mononuclear cell count was performed by automated cell counter (Sysmex KX-21, Japan). The viability was tested with Trypan Blue; dye exclusion method. The CD34 þ cells count was performed by using flow cytometery analysis. The Pearson correlation test was used for analysis. The level of statistically significance was set at 0.01 two sided for Pearson correlation (p < 0.01). Total 500 UCB samples were analyzed, concerning newborn sex distribution, 56.4% infants were males and 43.6% infants were females. The median gestational age was 38 weeks (mean 37.57 AE 1.82

Research paper thumbnail of Does Umbilical Cord Blood-derived CD34+ Cell Concentration Depend on the Weight and Sex of a Full-term Infant?

Journal of Pediatric Hematology/Oncology, 2012

Umbilical cord blood has recently been considered as an alternative source of hematopoietic proge... more Umbilical cord blood has recently been considered as an alternative source of hematopoietic progenitor cells for clinical application. Patient survival in allogenic cord blood transplantation is critically dependent on the cord blood-derived total nucleated cell count and the total CD34 cell count/kg of the body weight. A number of factors such as maternal age, gestational age, newborn&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s sex and weight, umbilical cord length, and placental weight can influence the volume, amount of mononuclear cells, and the CD34 cell concentration. Cord blood was collected from normal vaginal and cesarean deliveries. It was immediately processed and assessed for the total nucleated cell count and CD34 cell concentration. Assessment of maternal and neonatal parameters such as gestational age, baby&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;s birth weight, and sex was carried out with the CD34 cell concentration. The mean CD34 cell concentration was 0.21±0.24% for the group with…

Research paper thumbnail of Correlation of umbilical cord blood volume with CD34+ cells concentration

International Journal of Blood Transfusion and Immunohematology, 2011

Aims: Umbilical cord blood (UCB) has been recently considered as an alternative source of hematop... more Aims: Umbilical cord blood (UCB) has been recently considered as an alternative source of hematopoitic progenitor cells for clinical application. The parameters commonly used to evaluate a UCB unit and predict transplant outcomes have been total nucleated cell count (TNCs), CD34+ cells concentration and total volume of cord blood collected. The volume of cord blood collection is also important for the high yield of CD34+ cells concentration and TNCs. The aim of the study was to find the correlation of umbilical cord blood volume with cord blood derived CD34+ cells concentration. Methods: Umbilical cord blood was collected from normal vaginal and cesarean deliveries. Total volume of cord blood collection was noted. It was immediately processed and assessed for total nucleated cells count and CD34+ cells concentration. Assessment of maternal and neonatal parameters such as mode of delivery, baby's birth weight and sex, cord blood volume and CD34+ cells concentration was made. Resu...

Research paper thumbnail of Role of miRNAs in cervical cancer: A comprehensive novel approach from pathogenesis to therapy

Journal of Gynecology Obstetrics and Human Reproduction, 2021

Human papillomaviruses (HPV) infection is a major causative agent and strongly associated with th... more Human papillomaviruses (HPV) infection is a major causative agent and strongly associated with the development of cervical cancer. Understanding the mechanisms of HPV-induced cervical cancer is extremely useful in therapeutic strategies for primary prevention (HPV vaccines) and secondary prevention (screening and diagnosis of precancerous lesions). However, due to the lack of proper implementation of screening programs in developing countries, cervical cancer is usually diagnosed at advanced stages that result in poor treatment responses. Nearly half of the patients will experience disease recurrence within two years post treatment. Therefore, it is vital to identify new tools for early diagnosis, prognosis, and treatment prediction. MicroRNAs (miRNAs) are small non-coding RNAs, implicated in posttranscriptional regulation of gene expression. Growing evidence has shown that abnormal miRNA expression is associated with cervical cancer progression, metastasis, and influences treatment outcomes. In this review, we provide comprehensive information about miRNA and their potential utility in cervical cancer diagnosis, prognosis, and clinical management to improve patient outcomes.