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Papers by robert stinson

Proceedings of the Forty-Eighth IEEE Holm Conference on Electrical Contacts

Si v e d a(es; Discussion of basic COUCeptS In iSOgOU "iSOta Ch analysis and methods for determin... more Si v e d a(es; Discussion of basic COUCeptS In iSOgOU "iSOta Ch analysis and methods for determining relative vorticity, divergence and velocity. 5. AUTHORCS; (Last name, lirst name, initial)

International Journal of Research in Pharmaceutical Sciences, 2020

The plants belong to the genus Wrightiaare widely distributed throughout the world. Wrightiatinct... more The plants belong to the genus Wrightiaare widely distributed throughout the world. Wrightiatinctoria (WT) plant species are being used in folk medicine for the treatment of several diseases. The review of the literature suggests that a good number of preclinical have confirmed the medicinal use of various Wrightiatinctoria species that have been mentioned in traditional medicine. The extract of Wrightiatinctoria was given daily to the rats, at doses of two hundred and 400 mg/kg along with thioacetamide to assess the affectivity of extract, against thioacetamide-induced hepatotoxicity. Serum samples were collected for analysis of various hepatoprotective parameters like aspartate transaminase, alanine transaminase, antacid phosphatase and total bilirubin, using commercially available test kits, together with morphological and histopathological indices in the liver of healthy and thioacetamide treated rats. Animals were sacrificed from each group, and their livers were dissected out ...

General Pharmacology: The Vascular System, 1977

Kelevan (a derivative of Kepone) was covalently bound to BSA (BSA-Kel) through a succinimide deri... more Kelevan (a derivative of Kepone) was covalently bound to BSA (BSA-Kel) through a succinimide derivative, and rabbits were immunized by injection of the hapten-protein conjugate in an emulsion of Freund's complete adjuvant. 2. Immunoglobulin fractions (Ig) were prepared from control and immune rabbit serum and Ig from BSA-Kel antiserum produced a complete reversal of Kepone inhibition of dog brain ATPase activities under given experimental conditions. 3. These results indicate that in studies with the chlorinated hydrocarbon pesticides (Kepone and DCPD):~ care must be takan during tissue preparation to minimize possible reversal of ATPase inhibition.

The Pennsylvania Magazine of History and Biography, 1977

Ida zJkf. Tarbell and the Ambiguities of Feminism T HE hit of the New York theatrical season in 1... more Ida zJkf. Tarbell and the Ambiguities of Feminism T HE hit of the New York theatrical season in 1905 was a political play by Charles Klein called The jQion and the (JKCouse, which opened at Broadway's Lyceum Theater on November 20. Theater audiences generally understood that Klein had based his drama on the recent assault against the Standard Oil trust by Ida M. Tarbell which zMcClure's ^Magazine had just finished serializing a few months earlier. It was easy to recognize in Klein's character, John B. Ryder, a thinly veiled John D. Rockefeller, and to see in the heroine, Shirley Rossmore, a partly disguised Ida Tarbell herself. Klein had had only moderate success as a writer of sentimental romances earlier, but The J(jon and the tJXCouse broke records. Its 686 continuous performances constituted the longest run of any American play on the New York stage up to that time, and soon promoters put together four road companies which toured the country for several months. 1 Klein's success had less to do with the quality of the play than it did with the rise of a newly critical public opinion and the fact that his characters were easily identifiable with prominent names appearing regularly in the newspapers and magazines of the Progressive Era. The plot concerned Shirley Rossmore, a young magazine journalist whose novel, The Cjreat ^American Octopus, attacks the ethics and empire of the most powerful robber baron in America, John Burkett Ryder. Dramatic dialogues between Rossmore and Ryder reveal the journalist courageously defending her father, a reform judge threatened with impeachment by Ryder's forces in

PLANT PHYSIOLOGY, 1969

A method is described for the preparation, from a subcellular particulate fraction of wax bean co... more A method is described for the preparation, from a subcellular particulate fraction of wax bean cotyledons, of a soluble enzyme system that is capable of converting 3-alanine to ethylene. In the presence of ATP, CoA, thiamine pyrophosphate, MgSO4, and pyridoxal phosphate, ethylene production is maximum at a 0.5 mNi concentration of 8-alanine. The system exhibits a pH optimum at 7.0 but when the pH is raised above 8, evolution of the volatile again increases and continues to do so up to pH 12. The enzyme system is stimulated by either NADPH or NADH; the concentration of NADPH necessary to obtain maximum activity is twice that of NADH. The requirement for a reducing agent is in agreement with the proposal that malonate semialdehyde, formed by an aminotransferase reaction from f3-alanine, is reduced to 83-hydroxypropionate. Both malonate semialdehyde and /-hydroxypropionate are better stimulators of production of the volatile in the soluble system than is f3-alanine, and .8-hydroxypropionate is a better stimulator than malonate semialdehyde. This system is also able to incorporate tritium from tritiated water into ethylene; this supports the proposal that ethylene is formed by the decarboxylation of acrylate, the latter being formed from f3-hydroxypropionate. Experiments with both cold and labeled malonate suggest that this compound stimulates ethylene production by acting as an end product inhibitor that prevents the loss of 'Malonate semialdehyde from the pathway. Malonate does not appear to serve as a precursor. Addition of cytopl-asmic enzymes to the enzymes) results in a considerable boost in (mdul/mg protein) is lowered from that of the 'soluble system' (prepared from particulate ethylene production, but the specific activity particulate enzymes alone.

PLANT PHYSIOLOGY, 1985

Smooth microsomal membranes were isolated from axes of soybean (Glycine max L. Merr.) seeds at th... more Smooth microsomal membranes were isolated from axes of soybean (Glycine max L. Merr.) seeds at the dehydration-tolerant (6 hours of imbibition) and dehydration-susceptible (36 hours of imbibition) stages of development and were exposed to free radicals in vitro using xanthinexanthine oxidase as a free radical source. Wide angle x-ray diffraction studies indicated that the lipid phase transition temperature of the microsomal membranes from the dehydration-tolerant axes increased from 7 to 14'C after exposure to free radicals, whereas those from the dehydration-susceptible axes increased from 9 to 40'C by the same free radical dose. The increased phase transition temperature was associated with a decrease in the phospholipid:sterol ratio, and an increase in the free fatty acid:phospholipid ratio. There was no significant change in total fatty acid saturation, which indicated that free radical treatment induced deesterification of membrane phospholipid, and not a change in fatty acid saturation. Similar compositional and structural changes have been previously observed in dehydration-injured soybean axes suggesting that dehydration may induce free radical injury to cellular membranes. Further, these membranes differ in their susceptibility to free radical injury, presumably reflecting compositional differences in the membrane since these membranes were exposed to free radicals in the absence of cytosol.

Cellular Immunology, 1977

Cells were collected from the gland of Harder (GH) and bone marrow (BM) of 14-, 21-, and 32-week-... more Cells were collected from the gland of Harder (GH) and bone marrow (BM) of 14-, 21-, and 32-week-old birds and were incubated with an Y-labeled rabbit antichicken Ig (IgG and IgM) serum. At 14 weeks of age the percentage of Ig' small lymphocytes (SL) in the GH and BM was similar. However, by 21 weeks of age Ig' SL in BM had increased to approximately 19% of total lymphocytes while the Ig+ SL in the GH represented less than 1.7% of the lymphocyte pool. A marked drop in the number of Ig' SL in BM occurred by 32 weeks of age. These data suggest that either the BM may be dependent on the bursa for maintenance of its Ig+ SL or it is unable to produce in situ or maintain Ig' SL with age. In the GH the predominant cell was the plasma cell (PC). Labeled PC (> 20 grains) exceeded SOY, of the total PC pool in the GH. These data contrast with the apparent deficiency of Ig receptors on murine PC. The maintenance of a large number of PC in the GH without the presence of Ig' SL illustrates the uniqueness of this gland.

American Quarterly, 1970

ROBERT STINSON Indiana University SS McClure's My A utobiography: The Progressive as Self-Ma... more ROBERT STINSON Indiana University SS McClure's My A utobiography: The Progressive as Self-Made Man "WELL SAMMY 'YOU GOT THERE,' " THE LETTER SAID. SS MCCLURE'S HIGH school German teacher had just read the March 1914 installment of the famous ...

PLANT PHYSIOLOGY, 1980

Membrane damage as a result of dehydration was studied in Lotus corniculatus L. cv. Carrol seeds ... more Membrane damage as a result of dehydration was studied in Lotus corniculatus L. cv. Carrol seeds which had been pregerminated for 0, 12, and 24 hours prior to dehydration. During reimbibition, desiccation-tolerant (0and 12-hour) seeds leaked relatively low quantities of all solutes (total electrolytes, potassium, phosphate, sugar, amino acid, and protein). Desiccation-sensitive (24-hour) seeds leaked higher levels, but evidence of selective permeability remained. Membrane damage was not manifested as a complete removal of the diffusion barrier, although its permeability properties were dramatically altered. Consequently, the plasmalemma was not ruptured or torn by the dehydration treatment, but a more subtle structural alteration occurred. The possibility that seed membranes form a hexagonal rather than a lamellar phase at moisture contents below 20% was investigated by x-ray diffraction. Phospholipids were extracted from desiccation-tolerant (0hour) and desiccation-sensitive (24-hour) seeds and hydrated to 5, 10, 20, and 40% water. This phospholipid-water system was examined using lowand wide-angle x-ray diffraction and was found to be exclusively lamellar, even at 5% water. Consequently, membrane damage and the leakage of cytoplasmic solutes from seeds cannot be explained by the formation of a hexagonal phase by membrane phospholipids. ' Financial assistance was provided by Grant A6760 from the Natural Sciences and Engineering Research Council of Canada.

PLANT PHYSIOLOGY, 1984

Axes of soybean seeds are tolerant to dehydration at 6 hours of imbibition, but susceptible to de... more Axes of soybean seeds are tolerant to dehydration at 6 hours of imbibition, but susceptible to dehydration injury if dried at 36 hours of imbibition. Smooth microsomal membranes were isolated from axes imbibed for 6 hours (dehydration tolerant state) and 36 hours (dehydration susceptible state) before and after dehydration treatment. The phase properties and the lipid composition of the membrane fraction were investigated. Wide angle x-ray diffraction patterns of microsomal membranes from axes imbibed for 6 or 36 hours indicated a liquid-crystalline to gel phase transition at approximately 7C. Membranes from axes dehydrated at 6 or 36 hours of imbibition and rehydrated for 2 hours exhibited a phase transition at 7°C and 47°C, respectively. Changes in fatty acid saturation did not account for the changes in phase properties. However, the increased phase transition temperature of the membranes from dehydration injured axes was associated with an increase in free fatty acid:phospholipid molar ratio and a decrease in phospholipid:sterol ratio. These results suggests that dehydration prompted a deesterification of the linkage between glycerol and fatty acid side chains of the phospholipid molecules in the membrane. The resultant increase in free fatty acid content in the membrane is thought to alter the fluidity and phase properties of the membrane and contribute to dehydrtion injury.

Pesticide Biochemistry and Physiology, 1979

Journal of Molecular Biology, 1985

Hemoglobin Alberta has an amino acid substitution at position 101 (Glu + Gly), a residue involved... more Hemoglobin Alberta has an amino acid substitution at position 101 (Glu + Gly), a residue involved in the xl/?2 contact region of both the deoxy and oxy conformers of normal adult hemoglobin. Oxygen equilibrium measurements of stripped hemoglobin Alberta at 20°C in the absence of phosphate revealed a high affinity (P,, = 0.75 mm Hg at pH 7), cooperative hemoglobin variant (n = 2.3 at pH 7) with a normal Bohr effect (-A log P,,/ApH(,-s, = O-65). The addition of inositol hexaphosphate resulted in a decrease in oxygen affinity (P,, = 8.2 mm Hg at pH 7), a slight increase in the value of n and an enhanced Bohr effect. Rapid mixing experiments reflected the equilibrium results. A rapid rate of carbon monoxide binding (I' = 7-O x lo5 M-l s-l) and a slow rate of overall oxygen dissociation (k = 15 s-l) was seen at pH 7 and 20°C in the absence of phosphate. Under these experimental conditions the tetramer stability of liganded and unliganded hemoglobin Alberta was investigated by spectrophotometric kinetic techniques. The 4K4 value (the liganded tetramer-dimer equilibrium dissociation constant) for hemoglobin Alberta was found to be 0.83 x 10e6 M compared to a 4K4 value for hemoglobin A of 2.3 x 10m6 M, indicating that the Alberta tetramer was less dissociated into dimers than the tetramer of hemoglobin A. The values of OK, (the unliganded tetramer-dimer equilibrium dissociation constant) for hemoglobin Alberta and hemoglobin A were also measured and found to be 2.5 x lo-* M and 1.5 x 10-l' M, respectively, demonstrating a greatly destabilized deoxyhemoglobin tetramer for hemoglobin Alberta compared to deoxyhemoglobin A. The functional and subunit dissociation properties of hemoglobin Alberta appear to be directly related to the dual role of the /3101 residue in stabilizing the tetrameric form of the liganded structure, while concurrently destabilizing the unliganded tetramer molecule.

Journal of Medical Ethics, 1981

Developmental & Comparative Immunology, 1979

A specialized cell was identified in the medulla of bursal follicles referred to as a secretory c... more A specialized cell was identified in the medulla of bursal follicles referred to as a secretory cell (SC). The SC, present at all ages was concentrated in the vicinity of the corticomedu]lary border. Each secretory cell near the nucleus usually possessed a single long process containing 3-10 dark granules surrounded by a membrane. The SC increased in number and apparent secretory activity after cyclophosphamide treatment. All medullary pores were filled with a darkly staining substance, apparently secreted by the SC.

Clinical Biochemistry, 1987

Utilizing their differential susceptibilities to inhibitors and heat, we determined the amounts o... more Utilizing their differential susceptibilities to inhibitors and heat, we determined the amounts of the placental, liver, and fetal-intestinal isoenzyme forms of alkaline phosphatase in 143 samples of normal amniotic fluid obtained at 14 to 18 weeks' gestation (1). For reliable results, it was necessary to standardize inhibition profiles for each pure isoenzyme in amniotic fluid. Total activity and the absolute amounts of placental and fetal-intestinal activities were significantly related to gestational age (p less than 0.05). These relationships that were absent when activities were expressed as percentages of the total. The mean isoenzyme composition of the 143 samples, expressed as a percentage of total alkaline phosphatase activity, was: placental, 3.4%; liver, 9.8% (maximum, 47%); and fetal intestinal, 87% (minimum, 53%). The presence of phosphate in the assay medium (13.5 mmol/L) profoundly and differentially inhibited the isoenzymes of alkaline phosphatase and changed the inhibition profiles of the tissue-specific enzymes; thus, it would not be feasible to use inhibitors to differentiate the forms. We therefore propose a phosphate-free technique for quantifying the isoenzymes of alkaline phosphatase in amniotic fluid obtained at 14 to 18 weeks' gestation, to achieve the highest predictive values in a prenatal diagnostic test for cystic fibrosis.

Clinica Chimica Acta, 1981

The alkaline phosphatases present in the human tissues liver, kidney, intestine, placenta and a s... more The alkaline phosphatases present in the human tissues liver, kidney, intestine, placenta and a serum from a patient with Paget's disease of bone have been purified to apparent homogeneity by affinity elution from a phosphonic acid-Sepharose derivative. Polyacrylamide gel electrophoresis in SDS gave subunit molecular weights ranging from 74 000 for the enzyme form placenta to 95 800 for the enzyme from kidney. The purified native and desialylated enzymes have been characterized by agar electrophoresis and isoelectric focusing. All five of the native enzymes behaved differently but the desialylated forms from liver, Paget's serum and kidney were indistinguishable in both systems. The desialylated enzyme from placenta and the enzyme from intestine behaved differently from each other and from the above mentioned group. The isoelectric points ranged from less than 4 for the native enzyme from liver to 7.01 for the desialylated forms of the enzymes from liver, kidney and Paget's serum. The effects of a number of factors on the thermostability of the purified enzymes were studied. Phosphate decreased the stability and human serum albumin increased the stability of most molecular forms. Desialylation had no effect on thermostability. These results and those from inhibition studies with L-phenylalanine, L-homoarginine, phosphate and vanadate support the 3-gene hypothesis advanced from structural studies.

Canadian Journal of Zoology, 1984

The structure and composition of baleen from seven species of whales was studied using tensiometr... more The structure and composition of baleen from seven species of whales was studied using tensiometry. X-ray diffraction, and elemental analysis. Baleen was found to be composed principally of amorphous and α-keratin. Hydroxyapatite (bone mineral, Ca10 (PO4)6 OH2) was present in all species. Certain elements, notably manganese, copper, boron, iron, and calcium were more highly concentrated in the fibers than in the matrix of the plate. The breaking strength of baleen plates from fin (Balaenoptera physalus), sei (B. borealis), and grey (Eschrichtius robustus) whales was comparable to that of buffalo horn, in the range of 2−9 × 106 N∙m−2. The stiffness of baleen was somewhat less than that of other keratinized tissues. Treatment with 10% (v/v) trichloroacetic acid for 8 days removed most of the calcium salts, denatured α-keratin, and made fin whale plates stronger and stiffer. Exposure to gasoline for 1.5 h or 14 days, crude oil for 8 days, or tar for 21 days resulted in loss of trace el...

Canadian Journal of Physiology and Pharmacology, 1970

The concepts of dialysance and clearance, as applied in hemodialysis, were closely examined. Stri... more The concepts of dialysance and clearance, as applied in hemodialysis, were closely examined. Strict application of Wolf's definition shows dialysance to be a flow-independent function of the membrane area and thickness as well as of the diffusion coefficient of the substance to be removed. Clearance is a flow-dependent function of dialysance except in a non-flow situation, where clearance equals dialysance. Flow-dependent clearance equations and time-dependent equations for removal of substances from blood, both with and without concurrent metabolic production, are developed for Kolff and Kiil hemodialyzers. Since clearance increases with increase of any variable, only practical considerations need be considered in hemodialyzer design.

Clinical …, 2001

The Canadian Society of Clinical Chemists (CSCC) and the Canadian Academy of Clinical Biochemistr... more The Canadian Society of Clinical Chemists (CSCC) and the Canadian Academy of Clinical Biochemistry (CACB) have recently implemented a new professional development program for its 400 members. The program's goals are: to evaluate and recognize professional ...