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482-486Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medi... more 482-486Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 µM) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented with BAP (4.44-22.69 µM), IAA (5.71 µM) and kinetin (4.65 µM). It was observed that shoot length increased to1-2 cm. WPM medium supplemented with NAA (2.69-10.74 µM) and IBA (4.90 µM) induced rooting within 20-25 days. Using the present protocol, 20-25 plantlets could be regenerated from single root explant within 3 to 4 months. The protocol has potential for large scale production of elite plants
Journal of Applied Biology & Biotechnology, 2014
Fruits of Garcinia indica, a medicinally important tree species from Western Ghats of India is a ... more Fruits of Garcinia indica, a medicinally important tree species from Western Ghats of India is a rich source of medicinally important (-) Hydroxycitric acid that shows anticholesterol and antiobesity activities. Since this species is polygamodioecious, sex of the plant can be identified only after flowering that takes approximately 7-8 yrs. From commercial and horticultural point of view, plantation of female trees is much preferred. In this study, an efficient protocol for propagation of female plants was developed using in vitro techniques. For establishment of sterile cultures, fungal contamination and browning of explants were the major hurdles. Season of plant material collection and culture vessels used for in vitro sprouting were also observed significant factors in establishment of sterile cultures. The use of WPM basal fortified with 2% sucrose and 8.87 µM BAP resulted maximum sterile and healthy buds. TDZ in the range 0.22-4.54 µM along with BAP induced multiple shoots. Highest 63% rooting was obtained on WPM medium with 4.90 µM IBA whereas, maximum 60% plantlets survived in green house after their transfer to soil.
Indian journal of experimental biology, 2008
Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supp... more Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 microM) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented with BAP (4.44-22.69 microM), IAA (5.71 microM) and kinetin (4.65 microM). It was observed that shoot length increased to 1-2 cm. WPM medium supplemented with NAA (2.69-10.74 microM) and IBA (4.90 microM) induced rooting within 20-25 days. Using the present protocol, 20-25 plantlets could be regenerated from single root explant within 3 to 4 months. The protocol has potential for large scale production of elite plants.
482-486Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medi... more 482-486Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 µM) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented with BAP (4.44-22.69 µM), IAA (5.71 µM) and kinetin (4.65 µM). It was observed that shoot length increased to1-2 cm. WPM medium supplemented with NAA (2.69-10.74 µM) and IBA (4.90 µM) induced rooting within 20-25 days. Using the present protocol, 20-25 plantlets could be regenerated from single root explant within 3 to 4 months. The protocol has potential for large scale production of elite plants
Journal of Applied Biology & Biotechnology, 2014
Fruits of Garcinia indica, a medicinally important tree species from Western Ghats of India is a ... more Fruits of Garcinia indica, a medicinally important tree species from Western Ghats of India is a rich source of medicinally important (-) Hydroxycitric acid that shows anticholesterol and antiobesity activities. Since this species is polygamodioecious, sex of the plant can be identified only after flowering that takes approximately 7-8 yrs. From commercial and horticultural point of view, plantation of female trees is much preferred. In this study, an efficient protocol for propagation of female plants was developed using in vitro techniques. For establishment of sterile cultures, fungal contamination and browning of explants were the major hurdles. Season of plant material collection and culture vessels used for in vitro sprouting were also observed significant factors in establishment of sterile cultures. The use of WPM basal fortified with 2% sucrose and 8.87 µM BAP resulted maximum sterile and healthy buds. TDZ in the range 0.22-4.54 µM along with BAP induced multiple shoots. Highest 63% rooting was obtained on WPM medium with 4.90 µM IBA whereas, maximum 60% plantlets survived in green house after their transfer to soil.
Indian journal of experimental biology, 2008
Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supp... more Roots of plantlets of Garcinia indica when cultured for long time on half strength MS medium supplemented with BAP (0.44-2.22 microM) showed production of de novo shoots. Roots attached to mother plant showed more number of shoots, while excised root segments produced lesser shoots. Shoots (0.5-0.8 cm) were transferred to elongation medium consisting of Woody Plant Medium (WPM) supplemented with BAP (4.44-22.69 microM), IAA (5.71 microM) and kinetin (4.65 microM). It was observed that shoot length increased to 1-2 cm. WPM medium supplemented with NAA (2.69-10.74 microM) and IBA (4.90 microM) induced rooting within 20-25 days. Using the present protocol, 20-25 plantlets could be regenerated from single root explant within 3 to 4 months. The protocol has potential for large scale production of elite plants.