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Indian Phytopathology, 2002
... Titre du document / Document title. Host range studies of Xanthomonas axonopodis pv. vesicato... more ... Titre du document / Document title. Host range studies of Xanthomonas axonopodis pv. vesicatoria, the pathogen of bacterial leaf spot of chillies. Auteur(s) / Author(s).SWAPNA SREE D. ; SREERAMULU A. ; Revue / Journal Title. ...
Isolation of the rhizosphere microflora from the groundnut fields was done by serial dilution met... more Isolation of the rhizosphere microflora from the groundnut fields was done by serial dilution method and the microorganisms isolated were screened for antagonistic effect against Aspergillus flavus. Two fungal strains namely Penicilliumsp, and Trichodermaasperellum and four bacterial strains namely Pseudomonas fluorescence, Bacillus megaterium, Bacillus subtilis and Bacillus sp. were isolated and identified through morphological and biochemical characteristics from rhizosphere of groundnut. Of the above Trichodermaasperellum among fungal isolate and Pseudomonas fluorescence from bacterial isolate showed more antagonistic effect against Aspergillus flavus and thus proved to be potent biocontrol agents .Two talc-based formulations one from Trichodermaasperellum and another from Pseudomonas fluorescence were developed as an integrated disease management package against Yellow mold caused by Aspergillus flavus in Groundnut (Arachis hypogea L.). Trichodermaasperellum was multiplied in vitro in molasses-yeast medium and the biomass along with the medium was incorporated into the talc. The talc was packed and stored in polythene bags. SimilarlyPseudomonas fluorescence was multiplied in nutrient broth and formulated into talc based product. This was packed and stored in polythene bags.
Isolation of the rhizosphere microflora from the groundnut fields was done by serial dilution met... more Isolation of the rhizosphere microflora from the groundnut fields was done by serial dilution method and the microorganisms isolated were screened for antagonistic effect against Aspergillus flavus. Two fungal strains namely Penicilliumsp, and Trichodermaasperellum and four bacterial strains namely Pseudomonas fluorescence, Bacillus megaterium, Bacillus subtilis and Bacillus sp. were isolated and identified through morphological and biochemical characteristics from rhizosphere of groundnut. Trichodermaasperellum among fungal isolate and Pseudomonas fluorescence from bacterial isolate showed more antagonistic effect against Aspergillus flavus and thus proved to be potent biocontrol agents .Of the two potent biocontrol agents, Trichodermaasperellum showed maximum inhibitory zone against Aspergillus flavus and hence molecular characterization of isolate of Trichodermaasperellum was carried out. These studies revealed that revealed 99.84% similarity with ITS sequence of T. asperellum (Gen Bank Accession No. KY225658.1) when compared with GenBank Database using DDBJ BLAST search programme.
Indian Phytopathology, 2002
... Titre du document / Document title. Host range studies of Xanthomonas axonopodis pv. vesicato... more ... Titre du document / Document title. Host range studies of Xanthomonas axonopodis pv. vesicatoria, the pathogen of bacterial leaf spot of chillies. Auteur(s) / Author(s).SWAPNA SREE D. ; SREERAMULU A. ; Revue / Journal Title. ...
Isolation of the rhizosphere microflora from the groundnut fields was done by serial dilution met... more Isolation of the rhizosphere microflora from the groundnut fields was done by serial dilution method and the microorganisms isolated were screened for antagonistic effect against Aspergillus flavus. Two fungal strains namely Penicilliumsp, and Trichodermaasperellum and four bacterial strains namely Pseudomonas fluorescence, Bacillus megaterium, Bacillus subtilis and Bacillus sp. were isolated and identified through morphological and biochemical characteristics from rhizosphere of groundnut. Of the above Trichodermaasperellum among fungal isolate and Pseudomonas fluorescence from bacterial isolate showed more antagonistic effect against Aspergillus flavus and thus proved to be potent biocontrol agents .Two talc-based formulations one from Trichodermaasperellum and another from Pseudomonas fluorescence were developed as an integrated disease management package against Yellow mold caused by Aspergillus flavus in Groundnut (Arachis hypogea L.). Trichodermaasperellum was multiplied in vitro in molasses-yeast medium and the biomass along with the medium was incorporated into the talc. The talc was packed and stored in polythene bags. SimilarlyPseudomonas fluorescence was multiplied in nutrient broth and formulated into talc based product. This was packed and stored in polythene bags.
Isolation of the rhizosphere microflora from the groundnut fields was done by serial dilution met... more Isolation of the rhizosphere microflora from the groundnut fields was done by serial dilution method and the microorganisms isolated were screened for antagonistic effect against Aspergillus flavus. Two fungal strains namely Penicilliumsp, and Trichodermaasperellum and four bacterial strains namely Pseudomonas fluorescence, Bacillus megaterium, Bacillus subtilis and Bacillus sp. were isolated and identified through morphological and biochemical characteristics from rhizosphere of groundnut. Trichodermaasperellum among fungal isolate and Pseudomonas fluorescence from bacterial isolate showed more antagonistic effect against Aspergillus flavus and thus proved to be potent biocontrol agents .Of the two potent biocontrol agents, Trichodermaasperellum showed maximum inhibitory zone against Aspergillus flavus and hence molecular characterization of isolate of Trichodermaasperellum was carried out. These studies revealed that revealed 99.84% similarity with ITS sequence of T. asperellum (Gen Bank Accession No. KY225658.1) when compared with GenBank Database using DDBJ BLAST search programme.