tse-chuan chou - Academia.edu (original) (raw)
Papers by tse-chuan chou
Journal of the Electrochemical Society, 2003
Effects of Electrochemical Deposition of α-Ni(OH)/Pt/Ti Electrodes for Ethanol Anodic Oxidation. ... more Effects of Electrochemical Deposition of α-Ni(OH)/Pt/Ti Electrodes for Ethanol Anodic Oxidation. [Journal of The Electrochemical Society 150, C385 (2003)]. Yu-Ching Weng, Tse-Chuan Chou. Abstract. The aim of this paper is ...
Biosensors & bioelectronics, Jan 15, 2015
This study reports a novel enzyme-free, label-free amperometric method for direct detection of he... more This study reports a novel enzyme-free, label-free amperometric method for direct detection of hemoglobin A1c (Hb(A1c)), a potent biomarker for diabetes diagnosis and prognosis. The method relies on an electrode modified with poly(3-aminophenylboronic acid) (PAPBA) nanoparticles (20-50 nm) and a sensing scheme named "binding-induced ion flux blocking." The PAPBA nanoparticles were characterized by FT-IR, XPS, TEM, and SEM. Being a polyaniline derivative, PAPBA showed an ion-dependent redox behavior, in which insertion or extraction of ions into or out of PABPA occurred for charge balance during the electron transfer process. The polymer allowed Hb(A1c) selectively bound to its surface via forming the cis-diol linkage between the boronic acid and sugar moieties. Voltammetric analyses showed that Hb(A1c) binding decreased the redox current of PAPBA; however, the binding did not alter the redox potentials and the apparent diffusivities of ions. This suggests that the redox cu...
Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005., 2005
Molecularly imprinted polymers, artificial antibodies, were synthesized by printing target biomol... more Molecularly imprinted polymers, artificial antibodies, were synthesized by printing target biomolecules on a monomer mixture and then forming a highly selective and stable polymer. Scanning electrochemical microscopy (SECM) was employed to measure the reduction current between horseradish peroxide (HRP) conjugated lysozyme antibody and ferrocenylmethol (FMA) after different lysozyme concentrations for rebinding were applied on the molecularly imprinted polymers. We found
Digestive diseases and sciences, 2000
We conducted this experiment to assess the effect of saline injection in electrochemical therapy.... more We conducted this experiment to assess the effect of saline injection in electrochemical therapy. Platinum electrodes using direct current were inserted into egg white or liver parenchyma. Pure water or 0.9%, 3%, or 26% sodium chloride were injected into various objects to compare with the control group (no injection). Power was set at 10 V. In the egg-white experiment, gas bubbles and coagulated protein developed around the electrodes. In ex vivo liver, frothy reddish debris developed around the cathodes, while a hardening and shrunken surface occurred around the anodes. The pH was 14 around the cathodes, 0 around the anodes. The electric current, the amount of coagulated protein, and the severity of tissue damage were all in proportion to the concentrations of the injected saline. The volume destroyed in the 26% saline group was 8.1 times larger than that of the control group. Therefore, injected saline, especially saturated saline, can enhance the effect of electrochemical therapy.
Sensors and Actuators B: Chemical, 2001
... An amperometric ethanol sensor by using nickel modified carbon-rod electrode. ... Available o... more ... An amperometric ethanol sensor by using nickel modified carbon-rod electrode. ... Available online 23 February 2001. Abstract. An amperometric ethanol sensor was developed by usingnickel modified carbon-rod electrode in an alkaline electrolyte. ...
Sensors and Actuators B-Chemical, 2007
The 2004 IEEE Asia-Pacific Conference on Circuits and Systems, 2004. Proceedings., 2004
We propose a portable and wireless data transmission potentiostat and further exert the pH sensor... more We propose a portable and wireless data transmission potentiostat and further exert the pH sensor to verify its performance. This potentiostat can be used to process the long-distance remote electrochemical signals of the amperometric sensor and the potentiometric sensor. Based on the popular general packet radio service (GPRS) communication systems, a personal digital assistant (PDA) cell phone, instead of personal
Thin Solid Films, 2008
... 517, Issue 16, 30 June 2009, Page 4699, Jian-Jia Huang, Weng-Sing Hwang, Yu-Ching Weng, Tse-C... more ... 517, Issue 16, 30 June 2009, Page 4699, Jian-Jia Huang, Weng-Sing Hwang, Yu-Ching Weng, Tse-Chuan Chou ... The electrochemical properties of the prepared electrodes were examined by cyclic voltammetry and chronoamperometry by using CHI 1000 potentiostat with CHI ...
![Research paper thumbnail of Corrigendum to “Determination of alcohols using a Ni–Pt alloy amperometric sensor” [Thin Solid Films 516 (2008) 5210–5216]](https://attachments.academia-assets.com/39515031/thumbnails/1.jpg)
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Sensors and Actuators B: Chemical, 2003
A solid-state calcium ion-selective electrode was developed using thick film metallization proces... more A solid-state calcium ion-selective electrode was developed using thick film metallization process. Silicone rubber or photoresist was combined with ionophore (ETH 129) forming calcium ion-selective membrane which was coated onto different surfaces of electrode. Super-Nernstian equilibrium relationship between the phase boundary potentials and calcium ion concentrations was observed in the silicone rubber-based membrane that was doped with 10 −7 M CaCl 2 treated montmorillonite. This equilibrium relationship was eliminated when the membrane was doped with 0.1 M CaCl 2 treated montmorillonite. The use of photoresist simplified the manufacturing process for the membrane. The memory effect of the silicone rubber-based membrane could be eliminated when the membrane was conditioned with 0.1 M CaCl 2 . The response time of the electrode with silicone rubber and montmorillonite modified silicone rubber were less than 20 s and the one with photoresist was less than 3 min. The sensitivity of these calcium ion-selective electrodes were around 30 mV per decade, and the selectivity were in the rang from −2.9 to −3.8 for Na + , K + and Mg 2+ ions based on fixed primary ion method (FPIM).
Sensors and Actuators B: Chemical, 2003
Sensitive porous tungsten oxide nano-crystalline based NO 2 sensor was fabricated by thin film mi... more Sensitive porous tungsten oxide nano-crystalline based NO 2 sensor was fabricated by thin film microfabrication technique. The sensitivity of this NO 2 sensor was at parts per billion (ppb) level. The nano-crystalline porous tungsten oxide film was prepared from WCl 6 by a sol-gel technique. The surface morphology and sensitivity to NO 2 of the tungsten oxide films calcined at various temperatures were investigated. The NO 2 adsorption behavior on the tungsten oxide surface was carried out by XPS measurement. Experimental results indicated that the tungsten oxide film calcined at 550 • C for 1 h showed the best performance as a sensing material to NO 2 , and the optimal operational temperature of the sensor was 300 • C. The sensor showed high sensitivity to low NO 2 concentration in the range from 50 to 550 ppb with relatively fast response time (∼3 min) and recovery time (∼1 min), respectively.
PLoS ONE, 2011
Background: The sensitivity of non-small cell lung cancer (NSCLC) patients to EGFR tyrosine kinas... more Background: The sensitivity of non-small cell lung cancer (NSCLC) patients to EGFR tyrosine kinase inhibitors (TKIs) is strongly associated with activating EGFR mutations. Although not as sensitive as patients harboring these mutations, some patients with wild-type EGFR (wtEGFR) remain responsive to EGFR TKIs, suggesting that the existence of unexplored mechanisms renders most of wtEGFR-expressing cancer cells insensitive.
Nanotechnology, 2006
Ribonuclease (RNase), an enzyme which degrades RNA, is ubiquitous in living organisms, can renatu... more Ribonuclease (RNase), an enzyme which degrades RNA, is ubiquitous in living organisms, can renature after autoclaving, and is difficult to inactivate. The removal of RNase is especially necessary for the reverse transcription-polymerase chain reaction (RT-PCR) and for in vitro transcription and translation. Typically, RNase inhibitors must be added to these reactions nowadays. Molecularly imprinted polymers (MIPs) could offer many advantages for removal of undesired enzymes, including high binding selectivity, stability, low cost, and facile synthesis. Surface imprinting, employing immobilized RNase, was used in this study to make the most effective use of the template molecules-clearly, inaccessible binding sites, no matter how well imprinted, are not useful for target binding. Different monomers and cross-linkers were used to synthesize RNase-templated MIPs, and the rebinding capacity of each composition was characterized. We found that using polyethylene glycol 400 dimethacrylate (PEG400DMA) gave the highest imprinting effectiveness (i.e. the highest RNase binding ratio between imprinted and non-imprinted polymers). However, including styrene monomer (50 wt%) gave polymers with the highest overall affinity for ribonuclease A (RNase A). Finally, isothermal titration calorimetry was used as an auxiliary tool to help elucidate the mechanisms of the binding of monomers to templates, and ligands to MIPs.
Microchemical Journal, 2009
Micro-contact imprinting has been used to form thin-film molecular imprints of ovalbumin (OVA) in... more Micro-contact imprinting has been used to form thin-film molecular imprints of ovalbumin (OVA) in polymers supported on glass slides. Thermocalorimetric data was used to optimise the choice of functional monomer and cross-linker to maximise selectivity and minimise non-specific recognition. A polymer comprising polyethyleneglycol 400 dimethacrylate (95 vol.%) and methacrylic acid (5 vol.%) showed both maximum recognition for OVA when made as a molecularly imprinted polymer (MIP), and minimal recognition when made as a non-imprinted, i.e. control polymer. OVA rebinding to the molecularly imprinted polymer, from a buffered 2 µM OVA solution, was 1.55 × 10 − 11 mol cm − 2 , while the control polymer showed 10-fold less re-binding, i.e. 0.154 × 10 − 11 mol cm − 2 . Experiments in which human serum albumin (HSA), conalbumin, ovomucoid or lysozyme, were re-bound to the polymers, either as single proteins or in competition with OVA, showed them to have low affinity for the polymer formulation used. Of the competing proteins examined, in non-competitive binding experiments, HSA showed the greatest affinity 0.45 × 10 − 11 mol cm − 2 for the OVA imprinted polymer. In two protein competition experiments, i.e. with OVA and a competing protein present at equal concentrations (2 µM), OVA binding to the OVA imprinted polymer was in all cases significantly greater than that of the competitor.
Materials Science and Engineering: A, 2003
Microfabrication of Al 2 O 3 , TiO 2 and ZrO 2 hollow particles were carried out by spray precipi... more Microfabrication of Al 2 O 3 , TiO 2 and ZrO 2 hollow particles were carried out by spray precipitation that the fast polymerization is induced from dehydration or acid Á/base neutralization on the interface of the sprayed droplet to form rigid shell structure, and then removing the remained solvent within the shell. High yield of the smooth hollow particles can be achieved by selecting a highly polymeric precursor, which has higher surface tension, viscosity and density than those of the precipitation agent. In this work, micro to nano scaled Al 2 O 3 , TiO 2 , and ZrO 2 hollow particles are obtained, respectively, by spraying their precursor solutions including: aluminum chlorohydrate, titanium chloride, and zirconly chloride into the precipitation agents including acetone, triethylamine and tributylamine. Subsequently, the remained water in the inner shell particles are allowed to vaporize carefully and calcinated. A bottle structure was demonstrated by increasing the momentum of sprayed droplets to impact with the precipitation agent. #
Journal of Power Sources, 2009
The synthesis of rod-like LiFePO4/C cathodes using template-free reverse micelle process is repor... more The synthesis of rod-like LiFePO4/C cathodes using template-free reverse micelle process is reported for high performance lithium batteries. We have demonstrated that the size of the primary particles could be controlled based on sintering temperature and sintering time ...
Journal of Power Sources, 2006
Journal of the Electrochemical Society, 2003
Effects of Electrochemical Deposition of α-Ni(OH)/Pt/Ti Electrodes for Ethanol Anodic Oxidation. ... more Effects of Electrochemical Deposition of α-Ni(OH)/Pt/Ti Electrodes for Ethanol Anodic Oxidation. [Journal of The Electrochemical Society 150, C385 (2003)]. Yu-Ching Weng, Tse-Chuan Chou. Abstract. The aim of this paper is ...
Biosensors & bioelectronics, Jan 15, 2015
This study reports a novel enzyme-free, label-free amperometric method for direct detection of he... more This study reports a novel enzyme-free, label-free amperometric method for direct detection of hemoglobin A1c (Hb(A1c)), a potent biomarker for diabetes diagnosis and prognosis. The method relies on an electrode modified with poly(3-aminophenylboronic acid) (PAPBA) nanoparticles (20-50 nm) and a sensing scheme named "binding-induced ion flux blocking." The PAPBA nanoparticles were characterized by FT-IR, XPS, TEM, and SEM. Being a polyaniline derivative, PAPBA showed an ion-dependent redox behavior, in which insertion or extraction of ions into or out of PABPA occurred for charge balance during the electron transfer process. The polymer allowed Hb(A1c) selectively bound to its surface via forming the cis-diol linkage between the boronic acid and sugar moieties. Voltammetric analyses showed that Hb(A1c) binding decreased the redox current of PAPBA; however, the binding did not alter the redox potentials and the apparent diffusivities of ions. This suggests that the redox cu...
Proceedings of the IEEE 31st Annual Northeast Bioengineering Conference, 2005., 2005
Molecularly imprinted polymers, artificial antibodies, were synthesized by printing target biomol... more Molecularly imprinted polymers, artificial antibodies, were synthesized by printing target biomolecules on a monomer mixture and then forming a highly selective and stable polymer. Scanning electrochemical microscopy (SECM) was employed to measure the reduction current between horseradish peroxide (HRP) conjugated lysozyme antibody and ferrocenylmethol (FMA) after different lysozyme concentrations for rebinding were applied on the molecularly imprinted polymers. We found
Digestive diseases and sciences, 2000
We conducted this experiment to assess the effect of saline injection in electrochemical therapy.... more We conducted this experiment to assess the effect of saline injection in electrochemical therapy. Platinum electrodes using direct current were inserted into egg white or liver parenchyma. Pure water or 0.9%, 3%, or 26% sodium chloride were injected into various objects to compare with the control group (no injection). Power was set at 10 V. In the egg-white experiment, gas bubbles and coagulated protein developed around the electrodes. In ex vivo liver, frothy reddish debris developed around the cathodes, while a hardening and shrunken surface occurred around the anodes. The pH was 14 around the cathodes, 0 around the anodes. The electric current, the amount of coagulated protein, and the severity of tissue damage were all in proportion to the concentrations of the injected saline. The volume destroyed in the 26% saline group was 8.1 times larger than that of the control group. Therefore, injected saline, especially saturated saline, can enhance the effect of electrochemical therapy.
Sensors and Actuators B: Chemical, 2001
... An amperometric ethanol sensor by using nickel modified carbon-rod electrode. ... Available o... more ... An amperometric ethanol sensor by using nickel modified carbon-rod electrode. ... Available online 23 February 2001. Abstract. An amperometric ethanol sensor was developed by usingnickel modified carbon-rod electrode in an alkaline electrolyte. ...
Sensors and Actuators B-Chemical, 2007
The 2004 IEEE Asia-Pacific Conference on Circuits and Systems, 2004. Proceedings., 2004
We propose a portable and wireless data transmission potentiostat and further exert the pH sensor... more We propose a portable and wireless data transmission potentiostat and further exert the pH sensor to verify its performance. This potentiostat can be used to process the long-distance remote electrochemical signals of the amperometric sensor and the potentiometric sensor. Based on the popular general packet radio service (GPRS) communication systems, a personal digital assistant (PDA) cell phone, instead of personal
Thin Solid Films, 2008
... 517, Issue 16, 30 June 2009, Page 4699, Jian-Jia Huang, Weng-Sing Hwang, Yu-Ching Weng, Tse-C... more ... 517, Issue 16, 30 June 2009, Page 4699, Jian-Jia Huang, Weng-Sing Hwang, Yu-Ching Weng, Tse-Chuan Chou ... The electrochemical properties of the prepared electrodes were examined by cyclic voltammetry and chronoamperometry by using CHI 1000 potentiostat with CHI ...
Sensors and Actuators B: Chemical, 2003
A solid-state calcium ion-selective electrode was developed using thick film metallization proces... more A solid-state calcium ion-selective electrode was developed using thick film metallization process. Silicone rubber or photoresist was combined with ionophore (ETH 129) forming calcium ion-selective membrane which was coated onto different surfaces of electrode. Super-Nernstian equilibrium relationship between the phase boundary potentials and calcium ion concentrations was observed in the silicone rubber-based membrane that was doped with 10 −7 M CaCl 2 treated montmorillonite. This equilibrium relationship was eliminated when the membrane was doped with 0.1 M CaCl 2 treated montmorillonite. The use of photoresist simplified the manufacturing process for the membrane. The memory effect of the silicone rubber-based membrane could be eliminated when the membrane was conditioned with 0.1 M CaCl 2 . The response time of the electrode with silicone rubber and montmorillonite modified silicone rubber were less than 20 s and the one with photoresist was less than 3 min. The sensitivity of these calcium ion-selective electrodes were around 30 mV per decade, and the selectivity were in the rang from −2.9 to −3.8 for Na + , K + and Mg 2+ ions based on fixed primary ion method (FPIM).
Sensors and Actuators B: Chemical, 2003
Sensitive porous tungsten oxide nano-crystalline based NO 2 sensor was fabricated by thin film mi... more Sensitive porous tungsten oxide nano-crystalline based NO 2 sensor was fabricated by thin film microfabrication technique. The sensitivity of this NO 2 sensor was at parts per billion (ppb) level. The nano-crystalline porous tungsten oxide film was prepared from WCl 6 by a sol-gel technique. The surface morphology and sensitivity to NO 2 of the tungsten oxide films calcined at various temperatures were investigated. The NO 2 adsorption behavior on the tungsten oxide surface was carried out by XPS measurement. Experimental results indicated that the tungsten oxide film calcined at 550 • C for 1 h showed the best performance as a sensing material to NO 2 , and the optimal operational temperature of the sensor was 300 • C. The sensor showed high sensitivity to low NO 2 concentration in the range from 50 to 550 ppb with relatively fast response time (∼3 min) and recovery time (∼1 min), respectively.
PLoS ONE, 2011
Background: The sensitivity of non-small cell lung cancer (NSCLC) patients to EGFR tyrosine kinas... more Background: The sensitivity of non-small cell lung cancer (NSCLC) patients to EGFR tyrosine kinase inhibitors (TKIs) is strongly associated with activating EGFR mutations. Although not as sensitive as patients harboring these mutations, some patients with wild-type EGFR (wtEGFR) remain responsive to EGFR TKIs, suggesting that the existence of unexplored mechanisms renders most of wtEGFR-expressing cancer cells insensitive.
Nanotechnology, 2006
Ribonuclease (RNase), an enzyme which degrades RNA, is ubiquitous in living organisms, can renatu... more Ribonuclease (RNase), an enzyme which degrades RNA, is ubiquitous in living organisms, can renature after autoclaving, and is difficult to inactivate. The removal of RNase is especially necessary for the reverse transcription-polymerase chain reaction (RT-PCR) and for in vitro transcription and translation. Typically, RNase inhibitors must be added to these reactions nowadays. Molecularly imprinted polymers (MIPs) could offer many advantages for removal of undesired enzymes, including high binding selectivity, stability, low cost, and facile synthesis. Surface imprinting, employing immobilized RNase, was used in this study to make the most effective use of the template molecules-clearly, inaccessible binding sites, no matter how well imprinted, are not useful for target binding. Different monomers and cross-linkers were used to synthesize RNase-templated MIPs, and the rebinding capacity of each composition was characterized. We found that using polyethylene glycol 400 dimethacrylate (PEG400DMA) gave the highest imprinting effectiveness (i.e. the highest RNase binding ratio between imprinted and non-imprinted polymers). However, including styrene monomer (50 wt%) gave polymers with the highest overall affinity for ribonuclease A (RNase A). Finally, isothermal titration calorimetry was used as an auxiliary tool to help elucidate the mechanisms of the binding of monomers to templates, and ligands to MIPs.
Microchemical Journal, 2009
Micro-contact imprinting has been used to form thin-film molecular imprints of ovalbumin (OVA) in... more Micro-contact imprinting has been used to form thin-film molecular imprints of ovalbumin (OVA) in polymers supported on glass slides. Thermocalorimetric data was used to optimise the choice of functional monomer and cross-linker to maximise selectivity and minimise non-specific recognition. A polymer comprising polyethyleneglycol 400 dimethacrylate (95 vol.%) and methacrylic acid (5 vol.%) showed both maximum recognition for OVA when made as a molecularly imprinted polymer (MIP), and minimal recognition when made as a non-imprinted, i.e. control polymer. OVA rebinding to the molecularly imprinted polymer, from a buffered 2 µM OVA solution, was 1.55 × 10 − 11 mol cm − 2 , while the control polymer showed 10-fold less re-binding, i.e. 0.154 × 10 − 11 mol cm − 2 . Experiments in which human serum albumin (HSA), conalbumin, ovomucoid or lysozyme, were re-bound to the polymers, either as single proteins or in competition with OVA, showed them to have low affinity for the polymer formulation used. Of the competing proteins examined, in non-competitive binding experiments, HSA showed the greatest affinity 0.45 × 10 − 11 mol cm − 2 for the OVA imprinted polymer. In two protein competition experiments, i.e. with OVA and a competing protein present at equal concentrations (2 µM), OVA binding to the OVA imprinted polymer was in all cases significantly greater than that of the competitor.
Materials Science and Engineering: A, 2003
Microfabrication of Al 2 O 3 , TiO 2 and ZrO 2 hollow particles were carried out by spray precipi... more Microfabrication of Al 2 O 3 , TiO 2 and ZrO 2 hollow particles were carried out by spray precipitation that the fast polymerization is induced from dehydration or acid Á/base neutralization on the interface of the sprayed droplet to form rigid shell structure, and then removing the remained solvent within the shell. High yield of the smooth hollow particles can be achieved by selecting a highly polymeric precursor, which has higher surface tension, viscosity and density than those of the precipitation agent. In this work, micro to nano scaled Al 2 O 3 , TiO 2 , and ZrO 2 hollow particles are obtained, respectively, by spraying their precursor solutions including: aluminum chlorohydrate, titanium chloride, and zirconly chloride into the precipitation agents including acetone, triethylamine and tributylamine. Subsequently, the remained water in the inner shell particles are allowed to vaporize carefully and calcinated. A bottle structure was demonstrated by increasing the momentum of sprayed droplets to impact with the precipitation agent. #
Journal of Power Sources, 2009
The synthesis of rod-like LiFePO4/C cathodes using template-free reverse micelle process is repor... more The synthesis of rod-like LiFePO4/C cathodes using template-free reverse micelle process is reported for high performance lithium batteries. We have demonstrated that the size of the primary particles could be controlled based on sintering temperature and sintering time ...
Journal of Power Sources, 2006