Jan van den Broek - Academia.edu (original) (raw)
Papers by Jan van den Broek
Frontiers in Psychology
Mental models influence how individuals think and act in relation to their external environment a... more Mental models influence how individuals think and act in relation to their external environment and have been identified as leverage points to address sustainability challenges. Given the importance of mental models, a new tool to assess mental models has been developed: the Mental Model Mapping Tool (M-Tool). M-Tool was designed to have a standardized format and to be user-friendly for low literacy populations, using pictograms and audio instructions. In this paper, we evaluate M-Tool’s application in two studies with Tanzanian fishers. In Study 1, we investigated M-tool’s convergent validity compared to standard interviewing methods (n = 30). Study 2 investigated M-Tool’s construct validity by relating mental model complexity to participants’ education level (n = 185), a relationship that has been well established. The findings show that (1) mental models produced with M-Tool are more complex than mental models obtained through interviewing techniques; (2) model composition is sim...
Parasites & Vectors, 2022
Background The main objective of this study was to determine the prevalence of nine vector-borne ... more Background The main objective of this study was to determine the prevalence of nine vector-borne pathogens or pathogen genera in roe deer (Capreolus capreolus) in the Netherlands, and to identify which host variables predict vector-borne pathogen presence in roe deer. The host variables examined were the four host factors ‘age category’, ‘sex’, ‘nutritional condition’ and ‘health status’, as well as ‘roe deer density’. Methods From December 2009 to September 2010, blood samples of 461 roe deer were collected and analysed by polymerase chain reaction (PCR) for the presence of genetic material from Anaplasma phagocytophilum, Bartonella spp., Babesia spp., Borrelia burgdorferi sensu lato (s.l.), Borrelia miyamotoi, Neoehrlichia mikurensis, Rickettsia spp., and epizootic haemorrhagic disease virus (EHDV), and by commercial enzyme-linked immunosorbent assay (ELISA) for antibodies against bluetongue virus (BTV). The possible associations of host factors and density with pathogen prevalenc...
Biological Conservation, 2020
With global increases in anthropogenic pressures on wildlife populations comes a responsibility t... more With global increases in anthropogenic pressures on wildlife populations comes a responsibility to manage them effectively. The assessment of marine ecosystem health is challenging and often relies on monitoring indicator species, such as cetaceans. Most cetaceans are however highly mobile and spend the majority of their time hidden from direct view, resulting in uncertainty on even the most basic population metrics. Here, we discuss the value of long-term and internationally combined stranding records as a valuable source of information on the demographic and mortality trends of the harbour porpoise (Phocoena phocoena) in the North Sea. We analysed stranding records (n = 16,181) from 1990 to 2017 and demonstrate a strong heterogeneous seasonal pattern of strandings throughout the North Sea, indicative of season-specific distribution or habitat use, and season-specific mortality. The annual incidence of strandings has increased since 1990, with a notable steeper rise particularly in the southern North Sea since 2005. A high density of neonatal strandings occurred specifically in the eastern North Sea, indicative of areas important for calving, and large numbers of juvenile males stranded in the southern parts, indicative of a population sink or reflecting higher male dispersion. These findings highlight the power of stranding records to detect potentially vulnerable population groups in time and space. This knowledge is vital for managers and can guide, for example, conservation measures such as the establishment of time-areaspecific limits to potentially harmful human activities, aiming to reduce the number and intensity of humanwildlife conflicts.
Animals, 2020
In this study, we examined the effects of pre-cooling on thermophysiological responses in horses ... more In this study, we examined the effects of pre-cooling on thermophysiological responses in horses exercising in moderate environmental conditions (average wet bulb globe temperature: 18.5 ± 3.8 °C). Ten international eventing horses performed moderate intensity canter training on two separate days, and were either pre-cooled with cold-water rinsing (5–9 °C for 8 ± 3 min; cooling) or were not pre-cooled (control). We determined velocity (V), heart rate (HR), rectal temperature (Tre,), shoulder and rump skin temperature (Tshoulder and Trump), plasma lactate concentration (LA), gross sweat loss (GSL), and local sweat rate (LSR), as well as sweat sodium, chloride and potassium concentrations. The effect of pre-cooling on Tre was dependent on time; after 20 min of exercise the effect was the largest (estimate: 0.990, 95% likelihood confidence intervals (95% CI): 0.987, 0.993) compared to the control condition, resulting in a lower median Tre of 0.3 °C. Skin temperature was also affected b...
Preventive Veterinary Medicine, 2020
In human sport science, the acute:chronic workload (ACWR) ratio is used to monitor an athlete's p... more In human sport science, the acute:chronic workload (ACWR) ratio is used to monitor an athlete's preparedness for competition and to assess injury risks. The aim of this study was to investigate whether acute and chronic workload calculations for external and internal loads (e.g. high-speed work distance and associated exertional effort) were associated with injury risk in elite eventing horses and to identify workloads performed by horses competing in different competition and at different fitness levels. Training load and injury data were collected from 58 international eventing horses (CCI2*-CCI5* level) over 1-3 years. A total of 94 individual competition seasons were monitored. During this period, heart rate (HR; beat/ min) and GPS data were collected of all their conditional training sessions and competitions. External load was determined as the distance (m) covered at high speed (HS 1 ; velocity between 6.6 and 9.5 m/s), and sprint speed (SS 2 ; velocity > 9.5 m/s). Internal load was calculated for HS and SS, using individualized training impulses (TRIMP 3 ;AU). For internal and external workload HS and SS the acute (1-week) and chronic (4-week) workloads were calculated and ACWR 4 determined. The injury data in relation to ACWR was modelled with a multilevel logistic regression. Akaike's information criterion was used for model reduction. Sixty-four soft tissue injuries were registered from a total of 2300 training sessions and competitions. External and internal workload at HS and SS were significantly affected by the year and fitness level of horses. Competition level and year significantly affected the distances covered at SS. The ACWR of high-speed distance of the present week (OR; 0.133, 95 % CI; 0.032, 0.484) and the previous week (OR 3.951, 95 % CI; 1.390, 12.498) were significantly associated with injury risk. Competition level and chronic workload had no significant effect on injuries. In agreement with findings in human athletes, acute spikes of workload in eventing horses increased the risk of injury. Evaluation of horses' workload can be used to design and effectively monitor training programs and can help to improve equine welfare by reducing injury risk.
Equine Veterinary Journal, 2020
BackgroundIn Warmblood horses, degenerative joint disease is involved in cervical malformation an... more BackgroundIn Warmblood horses, degenerative joint disease is involved in cervical malformation and malarticulation (CVM). The degree of contribution of articular process joint (APJ) osteochondrosis (OC) is not clear.Objectives(a) To explore the presence of predilection sites for APJ OC in cervical and cranial thoracic vertebral columns of Warmblood foals and (b) to examine the correlation of such a site with the predilection site of CVM.Study designCase series.MethodsSeven hundred APJ facets of C2 to T2 of 29 foals (11 months gestation to 12 months [median age 7 days; range 365 days; 95% confidence interval [95% CI] 2‐47 days]) were examined for OC and prevalence between joints, and the predilection site for CVM and the cranial cervical vertebral column were evaluated.ResultsAbout 20.6% of facets revealed OC. There was no predilection site. Prevalence decreased with age up to 1 year (odds ratio [OR] 0.997; (95% CI 0.975‐0.998)) but not up to 5 months. Severity increased with age in ...
Journal of Veterinary Internal Medicine, 2018
BackgroundOrganic dust is associated with Equine asthma. Ionization should reduce airborne dust l... more BackgroundOrganic dust is associated with Equine asthma. Ionization should reduce airborne dust levels.ObjectivesTo determine the effect of ionization of air, type of bedding, and feed on the levels of airborne dust, endotoxin, and fungal colonies in horse stables.Animals24 healthy University‐owned horses occupied the stables.MethodsA randomized controlled cross‐over study. Four units with 6 stables were equipped with an ionization installation (25 VA, 5000 Volt Direct Current). Horses were kept either on wood shavings and fed haylage (2 units), or on straw and fed dry hay (2 units). Measurements were performed with and without activated ionization, during daytime and nighttime, repeatedly over the course of a week and repeatedly during 4‐6 weeks. Statistical analysis was performed using a mixed effect model with Akaike's Information Criterion for model reduction and 95% profile (log) likelihood confidence intervals (CI).ResultsIonization did not alter concentrations of dust, en...
Emerging Infectious Diseases
To increase knowledge of tick-borne encephalitis virus (TBEV) circulation in the Netherlands, we ... more To increase knowledge of tick-borne encephalitis virus (TBEV) circulation in the Netherlands, we conducted serosurveillance in roe deer (Capreolus capreolus) during 2017 and compared results with those obtained during 2010. Results corroborate a more widespread occurrence of the virus in 2017. Additional precautionary public health measures have been taken.
Emerging microbes & infections, Jan 4, 2018
Ranaviruses are pathogenic viruses for poikilothermic vertebrates worldwide. The identification o... more Ranaviruses are pathogenic viruses for poikilothermic vertebrates worldwide. The identification of a common midwife toad virus (CMTV) associated with massive die-offs in water frogs (Pelophylax spp.) in the Netherlands has increased awareness for emerging viruses in amphibians in the country. Complete genome sequencing of 13 ranavirus isolates collected from ten different sites in the period 2011-2016 revealed three CMTV groups present in distinct geographical areas in the Netherlands. Phylogenetic analysis showed that emerging viruses from the northern part of the Netherlands belonged to CMTV-NL group I. Group II and III viruses were derived from the animals located in the center-east and south of the country, and shared a more recent common ancestor to CMTV-amphibian associated ranaviruses reported in China, Italy, Denmark, and Switzerland. Field monitoring revealed differences in water frog host abundance at sites where distinct ranavirus groups occur; with ranavirus-associated d...
Journal of dairy science, 2007
In this study, the contribution of management practices, herd characteristics, and seasonal varia... more In this study, the contribution of management practices, herd characteristics, and seasonal variables to the herd somatic cell count (SCC) was quantified in herds with low (<150,000 cells/mL), medium (150,000-200,000 cells/mL), and high (>200,000 cells/mL) herd SCC (HSCC). Selection of the variables was performed using a linear mixed effect model; HSCC was calculated as the arithmetic mean of the individual cow's SCC. The data concerning management practices were derived from 3 questionnaires on mastitis prevention and management practices on 246 Dutch dairy farms. The monthly Dairy Herd Improvement test data of these 246 farms were used to calculate the herd characteristics and seasonal effects. None of the management practices were associated with HSCC in all 3 HSCC categories. Some variables only had a significant association with HSCC in one HSCC category, such as dry premilking treatment (-9,100 cells/mL in the low HSCC category) or feeding calves with high SCC milk (...
Journal of Dairy Science, 2011
Journal of Medical Entomology, 2013
Accurate estimation of population size is key to understanding the ecology of disease vectors, as... more Accurate estimation of population size is key to understanding the ecology of disease vectors, as well as the epidemiology of the pathogens they carry and to plan effective control activities. Population size can be estimated through markÐreleaseÐrecapture (MRR) experiments that are based on the assumption that the ratio of recaptured individuals to the total captures approximates the ratio of marked individuals released to the total population. However, methods to obtain population size estimates usually consider pooled data and are often based on the total number of marked and unmarked captures. We here present a logistic regression model, based on the principle of the well-known FisherÐFord method, speciÞc for MRR experiments where the information available is the number of marked mosquitoes released, the number of marked and unmarked mosquitoes caught in each trap and on each day, and the geographic coordinates of the traps. The model estimates population size, taking into consideration the distance between release points and traps, the time between release and recapture, and the loss of marked mosquitoes to death or dispersal. The performance and accuracy of the logistic regression model has been assessed using simulated data from known population sizes. We then applied the model to data from MRR experiments with Aedes albopictus Skuse performed on the campus of "Sapienza" University in Rome (Italy).
BMC Veterinary Research, 2013
Background: Eventing is generally recognized as a challenging equestrian discipline and wastage f... more Background: Eventing is generally recognized as a challenging equestrian discipline and wastage figures for this discipline are relatively high. There is a need for information that provides insight into the causes of wastage and withdrawal from competition, for animal welfare and economic reasons. The aim of the present investigation was to conduct a prospective study following the entire national selection of event horses (n = 20) and ponies (n = 9) in the Netherlands that prepared for the European Championship in 2010 (ponies) and 2011 (horses), noting causes of withdrawal and monitoring fitness using standardized exercise tests (SETs), with heart rate (HR; beats/min), speed (V; m/s) and plasma lactate concentrations (LA; mmol/L) as measured parameters. Results: In SET-I, performed at the beginning of the season, horses (n = 17) had a mean V LA4 (V at LA 4 mmol/L) of 10.3 ± 0.4 m/s with a mean V 200 (V at 200 beats/min) of 11.4 ± 0.8 m/s and ponies (n = 9) a mean V LA4 of 7.8 ± 0.9 m/s and V 200 of 9.6 ± 0.7 m/s. Before SET-II, performed six weeks before the European Championship, 16/20 horses and 6/9 ponies were withdrawn. The most common reason for withdrawal was locomotor injury (9/16 horses, 4/6 ponies; P < 0.001 and P = 0.011, respectively). Other reasons included an animal 'not meeting the competition criteria' (4/16 horses, 2/6 ponies) and being sold (3/16 horses). Animals were divided on the basis of V LA4 and recovery-HR during SET-I into good and average performers. Average performers were significantly more likely to be injured (50.0%) than good performers (0%, P = 0.05). In a subpopulation of ten horses, in which all condition training sessions were evaluated for HR and speed, HR peak was significantly lower in horses that stayed sound (186 ± 9 beats/min) compared with horses withdrawn from training and competition because of injury (201 ± 5 beats/min; P = 0.016). Conclusions: Of the national selection, 45% of all animals were unavailable for the European Championship because of locomotor injuries. Field tests were useful in assessing the potential injury risk, as individuals with better fitness indices (good performers) were less likely to become injured than average performers. Furthermore, monitoring of training sessions showed predictive value for future injuries, as horses withdrawn because of injury later on showed already higher peak HRs during condition training than horses that stayed sound. Therefore the increase in peak HR seemed to precede visible lameness in a horse.
Applied and Environmental Microbiology, 2005
Campylobacter species are frequently identified as a cause of human gastroenteritis, often from e... more Campylobacter species are frequently identified as a cause of human gastroenteritis, often from eating or mishandling contaminated poultry products. Quantitative knowledge of transmission of Campylobacter in broiler flocks is necessary, as this may help to determine the moment of introduction of Campylobacter in broiler flocks more precisely. The aim of this study was to determine the transmission rate parameter in broiler flocks. Four experiments were performed, each with four Campylobacter- inoculated chicks housed with 396 contact chicks per group. Colonization was monitored by regularly testing fecal samples for Campylobacter . A mathematical model was used to quantify the transmission rate, which was determined to be 1.04 new cases per colonized chick per day. This would imply that, for example, in a flock of 20,000 broilers, the prevalence of Campylobacter would increase from 5% to 95% within 6 days after Campylobacter introduction. The model and the estimated transmission rat...
American Journal of Veterinary Research, 2005
Objective—To determine incidence, risk factors, and heritability estimates of hind limb lameness ... more Objective—To determine incidence, risk factors, and heritability estimates of hind limb lameness caused by hip dysplasia in a birth cohort of Boxers. Animals—1,733 Boxers from 325 litters. Procedure—Status of Boxers with respect to clinical signs of canine hip dysplasia (cCHD) was registered during an 8-year period. Survival analysis accounted for dogs lost to follow-up. Effective heritability for developing cCHD was estimated by use of a proportional hazard model on the basis of the Weibull distribution. Parametric survival models were developed to identify the influence of potential risk factors. Results—Cumulative hazard rate for cCHD from 7 weeks to 8 years of age was 8.5%. Dogs that were kept on a floor covered with a slippery material were 1.6 times as likely to develop cCHD, compared with dogs kept on a nonslippery floor. Risk of cCHD doubled in dogs from litters with a high preweaning mortality rate. Dogs that were neutered at 6 months prior to a diagnosis of CHD were 1.5 ti...
Animals
Young Friesian stallions have to complete a 10-week training program (70-day test) for acceptance... more Young Friesian stallions have to complete a 10-week training program (70-day test) for acceptance as a breeding stallion. Part one of this study showed that the 70-day test was too intense and led to reduced fitness. In the present (part two) study, the effects of an adapted training program were studied. Training frequency and minutes of cantering per week were lowered compared with part one. The external workload (EW) of sixteen stallions (3.4 ± 0.8 years) was monitored during the 6-weeks before testing (preparation period) as well as during the 70-day test. Standardized exercise tests (SETs) were performed in week 1 (SET-I), 6 (SET-II), and 10 (SET-III) of the 70-day test, measuring heart rate (HR) and plasma lactate concentration (LA). Linear mixed effect models were used to analyze the EW, and the HR and LA related to the SETs. The EW increased from the preparation period to the 70-day test; this increase in the EW was lower compared with the original training program. The hors...
Preventive Veterinary Medicine, 2021
Culling of underperforming dairy cows by replacement heifers is a fundamental part of Dutch dairy... more Culling of underperforming dairy cows by replacement heifers is a fundamental part of Dutch dairy farm management. Changes in national agricultural policies can influence farmers' culling decisions. The objective of this study was to analyse the relevancy of cow-level risk factors for survival of Dutch dairy cows under perturbations due to national policy changes related to the-milk quota abolishment of 2015 and the phosphate regulations since 2017. For this purpose, an accelerated failure time model was fitted on-longitudinal dairy cows' data at national level covering the period 2009− 2019. The associated cow-level risk factors for culling such as lactation value (relative production level), parity number, rolling average of inseminations over all parities, very high fat-protein ratio (highFPR) and very low fat-protein ratio (lowFPR) in early lactation, test-day somatic cell count, were fitted in the model. Along with these, a factor representing three target policy periods, namely Milk Quota period (MQ), Post-Milk Quota period (PMQ) and Phosphate regulation period (PH) were fitted. The mean survival age for all producing cows was 441 weeks overall. The predicted median survival time for the policy periods MQ, PMQ and PH were 273 weeks, 271 weeks and 256 weeks, respectively. Risk factors such as lactation value, parity and highFPR, rolling average of inseminations over all parities were positively associated with survival time in all three policy periods. Risk factors such as test-day somatic cell count and lowFPR were negatively associated with survival time in all three policy periods. In conclusion, this study demonstrated the differences in survival of Dutch dairy cows in response to changing agricultural policy. The association of cowlevel risk factors for culling was consistent across the three evaluated policy periods.
Animals, 2021
Obesity has been associated with altered reproductive activity in mares, and may negatively affec... more Obesity has been associated with altered reproductive activity in mares, and may negatively affect fertility. To examine the influence of long-term high-energy (HE) feeding on fertility, Shetland pony mares were fed a diet containing 200% of net energy (NE) requirements during a three-year study. The incidence of hemorrhagic anovulatory follicles (HAF) and annual duration of cyclicity were compared to those in control mares receiving a maintenance diet. Day-7 embryos were flushed and transferred between donor and recipient mares from both groups; the resulting conceptuses were collected 21 days after transfer to assess conceptus development. HE mares became obese, and embryos recovered from HE mares were more likely to succumb to early embryonic death. The period of annual cyclicity was extended in HE compared to control mares in all years. The incidence of HAFs did not consistently differ between HE and control mares. No differences in embryo morphometric parameters were apparent. ...
Journal of Applied Statistics, 2020
Journal of Animal Science, 1994
The purpose of this study was to find the economically optimal period of first conception in gilt... more The purpose of this study was to find the economically optimal period of first conception in gilts, addressing the issues of lifetime reproductive performance and expected herd life. A profit equation was used to combine the effects into one economic parameter. The data were from 14,910 gilts on 54 farms throughout The Netherlands. The average number of pigs born alive in the first litter increased with older age at conception. In the second litter a similar, but much smaller, effect was observed. Age at first conception had no effect on number of pigs born alive in the third or greater litter. Gilts bred at an older age had a shorter expected herd life than gilts bred at a younger age. Evaluating the reasons for culling revealed that infertility became a more important reason with increasing age at first conception. The proportion culled for infertility increased linearly from 18% at conception on d 200 to 24.5% at conception on d 300. Combining the effect of litter size and herd life led to the conclusion that the profit per gilt (sow) was not significantly affected by her age at first conception. It is concluded that the optimal economic age at first conception was considered to be approximately 200 to 220 d of age when the cost of housing and feed of the gilt from entry to first conception were taken into account.
Frontiers in Psychology
Mental models influence how individuals think and act in relation to their external environment a... more Mental models influence how individuals think and act in relation to their external environment and have been identified as leverage points to address sustainability challenges. Given the importance of mental models, a new tool to assess mental models has been developed: the Mental Model Mapping Tool (M-Tool). M-Tool was designed to have a standardized format and to be user-friendly for low literacy populations, using pictograms and audio instructions. In this paper, we evaluate M-Tool’s application in two studies with Tanzanian fishers. In Study 1, we investigated M-tool’s convergent validity compared to standard interviewing methods (n = 30). Study 2 investigated M-Tool’s construct validity by relating mental model complexity to participants’ education level (n = 185), a relationship that has been well established. The findings show that (1) mental models produced with M-Tool are more complex than mental models obtained through interviewing techniques; (2) model composition is sim...
Parasites & Vectors, 2022
Background The main objective of this study was to determine the prevalence of nine vector-borne ... more Background The main objective of this study was to determine the prevalence of nine vector-borne pathogens or pathogen genera in roe deer (Capreolus capreolus) in the Netherlands, and to identify which host variables predict vector-borne pathogen presence in roe deer. The host variables examined were the four host factors ‘age category’, ‘sex’, ‘nutritional condition’ and ‘health status’, as well as ‘roe deer density’. Methods From December 2009 to September 2010, blood samples of 461 roe deer were collected and analysed by polymerase chain reaction (PCR) for the presence of genetic material from Anaplasma phagocytophilum, Bartonella spp., Babesia spp., Borrelia burgdorferi sensu lato (s.l.), Borrelia miyamotoi, Neoehrlichia mikurensis, Rickettsia spp., and epizootic haemorrhagic disease virus (EHDV), and by commercial enzyme-linked immunosorbent assay (ELISA) for antibodies against bluetongue virus (BTV). The possible associations of host factors and density with pathogen prevalenc...
Biological Conservation, 2020
With global increases in anthropogenic pressures on wildlife populations comes a responsibility t... more With global increases in anthropogenic pressures on wildlife populations comes a responsibility to manage them effectively. The assessment of marine ecosystem health is challenging and often relies on monitoring indicator species, such as cetaceans. Most cetaceans are however highly mobile and spend the majority of their time hidden from direct view, resulting in uncertainty on even the most basic population metrics. Here, we discuss the value of long-term and internationally combined stranding records as a valuable source of information on the demographic and mortality trends of the harbour porpoise (Phocoena phocoena) in the North Sea. We analysed stranding records (n = 16,181) from 1990 to 2017 and demonstrate a strong heterogeneous seasonal pattern of strandings throughout the North Sea, indicative of season-specific distribution or habitat use, and season-specific mortality. The annual incidence of strandings has increased since 1990, with a notable steeper rise particularly in the southern North Sea since 2005. A high density of neonatal strandings occurred specifically in the eastern North Sea, indicative of areas important for calving, and large numbers of juvenile males stranded in the southern parts, indicative of a population sink or reflecting higher male dispersion. These findings highlight the power of stranding records to detect potentially vulnerable population groups in time and space. This knowledge is vital for managers and can guide, for example, conservation measures such as the establishment of time-areaspecific limits to potentially harmful human activities, aiming to reduce the number and intensity of humanwildlife conflicts.
Animals, 2020
In this study, we examined the effects of pre-cooling on thermophysiological responses in horses ... more In this study, we examined the effects of pre-cooling on thermophysiological responses in horses exercising in moderate environmental conditions (average wet bulb globe temperature: 18.5 ± 3.8 °C). Ten international eventing horses performed moderate intensity canter training on two separate days, and were either pre-cooled with cold-water rinsing (5–9 °C for 8 ± 3 min; cooling) or were not pre-cooled (control). We determined velocity (V), heart rate (HR), rectal temperature (Tre,), shoulder and rump skin temperature (Tshoulder and Trump), plasma lactate concentration (LA), gross sweat loss (GSL), and local sweat rate (LSR), as well as sweat sodium, chloride and potassium concentrations. The effect of pre-cooling on Tre was dependent on time; after 20 min of exercise the effect was the largest (estimate: 0.990, 95% likelihood confidence intervals (95% CI): 0.987, 0.993) compared to the control condition, resulting in a lower median Tre of 0.3 °C. Skin temperature was also affected b...
Preventive Veterinary Medicine, 2020
In human sport science, the acute:chronic workload (ACWR) ratio is used to monitor an athlete's p... more In human sport science, the acute:chronic workload (ACWR) ratio is used to monitor an athlete's preparedness for competition and to assess injury risks. The aim of this study was to investigate whether acute and chronic workload calculations for external and internal loads (e.g. high-speed work distance and associated exertional effort) were associated with injury risk in elite eventing horses and to identify workloads performed by horses competing in different competition and at different fitness levels. Training load and injury data were collected from 58 international eventing horses (CCI2*-CCI5* level) over 1-3 years. A total of 94 individual competition seasons were monitored. During this period, heart rate (HR; beat/ min) and GPS data were collected of all their conditional training sessions and competitions. External load was determined as the distance (m) covered at high speed (HS 1 ; velocity between 6.6 and 9.5 m/s), and sprint speed (SS 2 ; velocity > 9.5 m/s). Internal load was calculated for HS and SS, using individualized training impulses (TRIMP 3 ;AU). For internal and external workload HS and SS the acute (1-week) and chronic (4-week) workloads were calculated and ACWR 4 determined. The injury data in relation to ACWR was modelled with a multilevel logistic regression. Akaike's information criterion was used for model reduction. Sixty-four soft tissue injuries were registered from a total of 2300 training sessions and competitions. External and internal workload at HS and SS were significantly affected by the year and fitness level of horses. Competition level and year significantly affected the distances covered at SS. The ACWR of high-speed distance of the present week (OR; 0.133, 95 % CI; 0.032, 0.484) and the previous week (OR 3.951, 95 % CI; 1.390, 12.498) were significantly associated with injury risk. Competition level and chronic workload had no significant effect on injuries. In agreement with findings in human athletes, acute spikes of workload in eventing horses increased the risk of injury. Evaluation of horses' workload can be used to design and effectively monitor training programs and can help to improve equine welfare by reducing injury risk.
Equine Veterinary Journal, 2020
BackgroundIn Warmblood horses, degenerative joint disease is involved in cervical malformation an... more BackgroundIn Warmblood horses, degenerative joint disease is involved in cervical malformation and malarticulation (CVM). The degree of contribution of articular process joint (APJ) osteochondrosis (OC) is not clear.Objectives(a) To explore the presence of predilection sites for APJ OC in cervical and cranial thoracic vertebral columns of Warmblood foals and (b) to examine the correlation of such a site with the predilection site of CVM.Study designCase series.MethodsSeven hundred APJ facets of C2 to T2 of 29 foals (11 months gestation to 12 months [median age 7 days; range 365 days; 95% confidence interval [95% CI] 2‐47 days]) were examined for OC and prevalence between joints, and the predilection site for CVM and the cranial cervical vertebral column were evaluated.ResultsAbout 20.6% of facets revealed OC. There was no predilection site. Prevalence decreased with age up to 1 year (odds ratio [OR] 0.997; (95% CI 0.975‐0.998)) but not up to 5 months. Severity increased with age in ...
Journal of Veterinary Internal Medicine, 2018
BackgroundOrganic dust is associated with Equine asthma. Ionization should reduce airborne dust l... more BackgroundOrganic dust is associated with Equine asthma. Ionization should reduce airborne dust levels.ObjectivesTo determine the effect of ionization of air, type of bedding, and feed on the levels of airborne dust, endotoxin, and fungal colonies in horse stables.Animals24 healthy University‐owned horses occupied the stables.MethodsA randomized controlled cross‐over study. Four units with 6 stables were equipped with an ionization installation (25 VA, 5000 Volt Direct Current). Horses were kept either on wood shavings and fed haylage (2 units), or on straw and fed dry hay (2 units). Measurements were performed with and without activated ionization, during daytime and nighttime, repeatedly over the course of a week and repeatedly during 4‐6 weeks. Statistical analysis was performed using a mixed effect model with Akaike's Information Criterion for model reduction and 95% profile (log) likelihood confidence intervals (CI).ResultsIonization did not alter concentrations of dust, en...
Emerging Infectious Diseases
To increase knowledge of tick-borne encephalitis virus (TBEV) circulation in the Netherlands, we ... more To increase knowledge of tick-borne encephalitis virus (TBEV) circulation in the Netherlands, we conducted serosurveillance in roe deer (Capreolus capreolus) during 2017 and compared results with those obtained during 2010. Results corroborate a more widespread occurrence of the virus in 2017. Additional precautionary public health measures have been taken.
Emerging microbes & infections, Jan 4, 2018
Ranaviruses are pathogenic viruses for poikilothermic vertebrates worldwide. The identification o... more Ranaviruses are pathogenic viruses for poikilothermic vertebrates worldwide. The identification of a common midwife toad virus (CMTV) associated with massive die-offs in water frogs (Pelophylax spp.) in the Netherlands has increased awareness for emerging viruses in amphibians in the country. Complete genome sequencing of 13 ranavirus isolates collected from ten different sites in the period 2011-2016 revealed three CMTV groups present in distinct geographical areas in the Netherlands. Phylogenetic analysis showed that emerging viruses from the northern part of the Netherlands belonged to CMTV-NL group I. Group II and III viruses were derived from the animals located in the center-east and south of the country, and shared a more recent common ancestor to CMTV-amphibian associated ranaviruses reported in China, Italy, Denmark, and Switzerland. Field monitoring revealed differences in water frog host abundance at sites where distinct ranavirus groups occur; with ranavirus-associated d...
Journal of dairy science, 2007
In this study, the contribution of management practices, herd characteristics, and seasonal varia... more In this study, the contribution of management practices, herd characteristics, and seasonal variables to the herd somatic cell count (SCC) was quantified in herds with low (<150,000 cells/mL), medium (150,000-200,000 cells/mL), and high (>200,000 cells/mL) herd SCC (HSCC). Selection of the variables was performed using a linear mixed effect model; HSCC was calculated as the arithmetic mean of the individual cow's SCC. The data concerning management practices were derived from 3 questionnaires on mastitis prevention and management practices on 246 Dutch dairy farms. The monthly Dairy Herd Improvement test data of these 246 farms were used to calculate the herd characteristics and seasonal effects. None of the management practices were associated with HSCC in all 3 HSCC categories. Some variables only had a significant association with HSCC in one HSCC category, such as dry premilking treatment (-9,100 cells/mL in the low HSCC category) or feeding calves with high SCC milk (...
Journal of Dairy Science, 2011
Journal of Medical Entomology, 2013
Accurate estimation of population size is key to understanding the ecology of disease vectors, as... more Accurate estimation of population size is key to understanding the ecology of disease vectors, as well as the epidemiology of the pathogens they carry and to plan effective control activities. Population size can be estimated through markÐreleaseÐrecapture (MRR) experiments that are based on the assumption that the ratio of recaptured individuals to the total captures approximates the ratio of marked individuals released to the total population. However, methods to obtain population size estimates usually consider pooled data and are often based on the total number of marked and unmarked captures. We here present a logistic regression model, based on the principle of the well-known FisherÐFord method, speciÞc for MRR experiments where the information available is the number of marked mosquitoes released, the number of marked and unmarked mosquitoes caught in each trap and on each day, and the geographic coordinates of the traps. The model estimates population size, taking into consideration the distance between release points and traps, the time between release and recapture, and the loss of marked mosquitoes to death or dispersal. The performance and accuracy of the logistic regression model has been assessed using simulated data from known population sizes. We then applied the model to data from MRR experiments with Aedes albopictus Skuse performed on the campus of "Sapienza" University in Rome (Italy).
BMC Veterinary Research, 2013
Background: Eventing is generally recognized as a challenging equestrian discipline and wastage f... more Background: Eventing is generally recognized as a challenging equestrian discipline and wastage figures for this discipline are relatively high. There is a need for information that provides insight into the causes of wastage and withdrawal from competition, for animal welfare and economic reasons. The aim of the present investigation was to conduct a prospective study following the entire national selection of event horses (n = 20) and ponies (n = 9) in the Netherlands that prepared for the European Championship in 2010 (ponies) and 2011 (horses), noting causes of withdrawal and monitoring fitness using standardized exercise tests (SETs), with heart rate (HR; beats/min), speed (V; m/s) and plasma lactate concentrations (LA; mmol/L) as measured parameters. Results: In SET-I, performed at the beginning of the season, horses (n = 17) had a mean V LA4 (V at LA 4 mmol/L) of 10.3 ± 0.4 m/s with a mean V 200 (V at 200 beats/min) of 11.4 ± 0.8 m/s and ponies (n = 9) a mean V LA4 of 7.8 ± 0.9 m/s and V 200 of 9.6 ± 0.7 m/s. Before SET-II, performed six weeks before the European Championship, 16/20 horses and 6/9 ponies were withdrawn. The most common reason for withdrawal was locomotor injury (9/16 horses, 4/6 ponies; P < 0.001 and P = 0.011, respectively). Other reasons included an animal 'not meeting the competition criteria' (4/16 horses, 2/6 ponies) and being sold (3/16 horses). Animals were divided on the basis of V LA4 and recovery-HR during SET-I into good and average performers. Average performers were significantly more likely to be injured (50.0%) than good performers (0%, P = 0.05). In a subpopulation of ten horses, in which all condition training sessions were evaluated for HR and speed, HR peak was significantly lower in horses that stayed sound (186 ± 9 beats/min) compared with horses withdrawn from training and competition because of injury (201 ± 5 beats/min; P = 0.016). Conclusions: Of the national selection, 45% of all animals were unavailable for the European Championship because of locomotor injuries. Field tests were useful in assessing the potential injury risk, as individuals with better fitness indices (good performers) were less likely to become injured than average performers. Furthermore, monitoring of training sessions showed predictive value for future injuries, as horses withdrawn because of injury later on showed already higher peak HRs during condition training than horses that stayed sound. Therefore the increase in peak HR seemed to precede visible lameness in a horse.
Applied and Environmental Microbiology, 2005
Campylobacter species are frequently identified as a cause of human gastroenteritis, often from e... more Campylobacter species are frequently identified as a cause of human gastroenteritis, often from eating or mishandling contaminated poultry products. Quantitative knowledge of transmission of Campylobacter in broiler flocks is necessary, as this may help to determine the moment of introduction of Campylobacter in broiler flocks more precisely. The aim of this study was to determine the transmission rate parameter in broiler flocks. Four experiments were performed, each with four Campylobacter- inoculated chicks housed with 396 contact chicks per group. Colonization was monitored by regularly testing fecal samples for Campylobacter . A mathematical model was used to quantify the transmission rate, which was determined to be 1.04 new cases per colonized chick per day. This would imply that, for example, in a flock of 20,000 broilers, the prevalence of Campylobacter would increase from 5% to 95% within 6 days after Campylobacter introduction. The model and the estimated transmission rat...
American Journal of Veterinary Research, 2005
Objective—To determine incidence, risk factors, and heritability estimates of hind limb lameness ... more Objective—To determine incidence, risk factors, and heritability estimates of hind limb lameness caused by hip dysplasia in a birth cohort of Boxers. Animals—1,733 Boxers from 325 litters. Procedure—Status of Boxers with respect to clinical signs of canine hip dysplasia (cCHD) was registered during an 8-year period. Survival analysis accounted for dogs lost to follow-up. Effective heritability for developing cCHD was estimated by use of a proportional hazard model on the basis of the Weibull distribution. Parametric survival models were developed to identify the influence of potential risk factors. Results—Cumulative hazard rate for cCHD from 7 weeks to 8 years of age was 8.5%. Dogs that were kept on a floor covered with a slippery material were 1.6 times as likely to develop cCHD, compared with dogs kept on a nonslippery floor. Risk of cCHD doubled in dogs from litters with a high preweaning mortality rate. Dogs that were neutered at 6 months prior to a diagnosis of CHD were 1.5 ti...
Animals
Young Friesian stallions have to complete a 10-week training program (70-day test) for acceptance... more Young Friesian stallions have to complete a 10-week training program (70-day test) for acceptance as a breeding stallion. Part one of this study showed that the 70-day test was too intense and led to reduced fitness. In the present (part two) study, the effects of an adapted training program were studied. Training frequency and minutes of cantering per week were lowered compared with part one. The external workload (EW) of sixteen stallions (3.4 ± 0.8 years) was monitored during the 6-weeks before testing (preparation period) as well as during the 70-day test. Standardized exercise tests (SETs) were performed in week 1 (SET-I), 6 (SET-II), and 10 (SET-III) of the 70-day test, measuring heart rate (HR) and plasma lactate concentration (LA). Linear mixed effect models were used to analyze the EW, and the HR and LA related to the SETs. The EW increased from the preparation period to the 70-day test; this increase in the EW was lower compared with the original training program. The hors...
Preventive Veterinary Medicine, 2021
Culling of underperforming dairy cows by replacement heifers is a fundamental part of Dutch dairy... more Culling of underperforming dairy cows by replacement heifers is a fundamental part of Dutch dairy farm management. Changes in national agricultural policies can influence farmers' culling decisions. The objective of this study was to analyse the relevancy of cow-level risk factors for survival of Dutch dairy cows under perturbations due to national policy changes related to the-milk quota abolishment of 2015 and the phosphate regulations since 2017. For this purpose, an accelerated failure time model was fitted on-longitudinal dairy cows' data at national level covering the period 2009− 2019. The associated cow-level risk factors for culling such as lactation value (relative production level), parity number, rolling average of inseminations over all parities, very high fat-protein ratio (highFPR) and very low fat-protein ratio (lowFPR) in early lactation, test-day somatic cell count, were fitted in the model. Along with these, a factor representing three target policy periods, namely Milk Quota period (MQ), Post-Milk Quota period (PMQ) and Phosphate regulation period (PH) were fitted. The mean survival age for all producing cows was 441 weeks overall. The predicted median survival time for the policy periods MQ, PMQ and PH were 273 weeks, 271 weeks and 256 weeks, respectively. Risk factors such as lactation value, parity and highFPR, rolling average of inseminations over all parities were positively associated with survival time in all three policy periods. Risk factors such as test-day somatic cell count and lowFPR were negatively associated with survival time in all three policy periods. In conclusion, this study demonstrated the differences in survival of Dutch dairy cows in response to changing agricultural policy. The association of cowlevel risk factors for culling was consistent across the three evaluated policy periods.
Animals, 2021
Obesity has been associated with altered reproductive activity in mares, and may negatively affec... more Obesity has been associated with altered reproductive activity in mares, and may negatively affect fertility. To examine the influence of long-term high-energy (HE) feeding on fertility, Shetland pony mares were fed a diet containing 200% of net energy (NE) requirements during a three-year study. The incidence of hemorrhagic anovulatory follicles (HAF) and annual duration of cyclicity were compared to those in control mares receiving a maintenance diet. Day-7 embryos were flushed and transferred between donor and recipient mares from both groups; the resulting conceptuses were collected 21 days after transfer to assess conceptus development. HE mares became obese, and embryos recovered from HE mares were more likely to succumb to early embryonic death. The period of annual cyclicity was extended in HE compared to control mares in all years. The incidence of HAFs did not consistently differ between HE and control mares. No differences in embryo morphometric parameters were apparent. ...
Journal of Applied Statistics, 2020
Journal of Animal Science, 1994
The purpose of this study was to find the economically optimal period of first conception in gilt... more The purpose of this study was to find the economically optimal period of first conception in gilts, addressing the issues of lifetime reproductive performance and expected herd life. A profit equation was used to combine the effects into one economic parameter. The data were from 14,910 gilts on 54 farms throughout The Netherlands. The average number of pigs born alive in the first litter increased with older age at conception. In the second litter a similar, but much smaller, effect was observed. Age at first conception had no effect on number of pigs born alive in the third or greater litter. Gilts bred at an older age had a shorter expected herd life than gilts bred at a younger age. Evaluating the reasons for culling revealed that infertility became a more important reason with increasing age at first conception. The proportion culled for infertility increased linearly from 18% at conception on d 200 to 24.5% at conception on d 300. Combining the effect of litter size and herd life led to the conclusion that the profit per gilt (sow) was not significantly affected by her age at first conception. It is concluded that the optimal economic age at first conception was considered to be approximately 200 to 220 d of age when the cost of housing and feed of the gilt from entry to first conception were taken into account.