vidya dharani - Academia.edu (original) (raw)
Papers by vidya dharani
BioMed research international, 2014
Microalgae lipid feedstock preparation cost was an important factor in increasing biodiesel fuel ... more Microalgae lipid feedstock preparation cost was an important factor in increasing biodiesel fuel hikes. This study was conducted with the concept of implementing an effluent wastewater as lipid production medium for microalgae cultivation. In our study textile dyeing industry effluent was taken as a lipid production medium for Neochloris sp. cultivation. The changes in physicochemical analysis of effluent before and after Neochloris sp. treatment were recorded using standard procedures and AAS analysis. There was especially a reduction in heavy metal like lead (Pb) concentration from 0.002 ppm to 0.001 ppm after Neochloris sp. treatment. Neochloris sp. cultivated in Bold Basal Medium (BBM) (specific algal medium) produced 41.93% total lipid and 36.69% lipid was produced in effluent based cultivation. Surprisingly Neochloris sp. cultivated in effluent was found with enhanced neutral lipid content, and it was confirmed by Nile red fluorescence assay. Further the particular enrichment ...
In the present study, microalgae were isolated from fresh pond water samples collected from Maman... more In the present study, microalgae were isolated from fresh pond water samples collected from Mamangam near Omalur Taluk, Salem. After performing the isolation procedure three microalgae isolates were successfully isolated .The synthetic medium namely B old Basal Medium (BBM), Tris acetate Phosphate (TAP), Bristol Medium were supplemented to all the three strains, further their biomass and lipid productivity are analysed. As a result one of the isolate namely Chlorella MS3 strain grown in Bristol medium found with higher percentage of total lipid. In particularly that Chlorella MS3 strain grown in Bristol was able to store neutral lipid proportion than other two strains. It was confirmed by Nile Red imaging studies. The Chlorella MS3 strain was selected for lipid enhancement procedures for biodiesel feed stock preparation.
Micro algae are photosynthetic miniatures capable of producing versatile compounds. It can provid... more Micro algae are photosynthetic miniatures capable of producing versatile compounds. It can provide biomass feed stock for different type of renewable fuels such as biodiesel, methane, hydrogen and ethanol. Among all, biodiesel can replace the present day fossil based fuel system. Other than bio-fuel production, microalgae can also serve for bioremediation and industry oriented processing. CO 2 sequestration from industrial flue gases by algae bio-fixation in turn reduces the green house gas emissions of an industry. Particularly algae biodiesel has no sulfur and more or less perform well than petroleum diesel. Emission gas contains reduced particulate matter, CO, hydrocarbon, SO 2. Even though it has enormous advantages, NO 2 emission from this diesel may be higher in some engine types. This review elaborates on the pros and cons of algal biodiesel usage in biodiesel sector.
Microbial inulinases have a great potential for industrial use in the production of fructose from... more Microbial inulinases have a great potential for industrial use in the production of fructose from inulin. Optimization of the growth parameters of the microbes is essential to obtain inulinase in sufficient quantity. The present work aimed to obtain inulinase from natural strains isolated from sugarcane fields and to optimize its growth parameters. In a total of ten isolates, four organisms Bacillus subtilis, Lactobacillus casei, Pseudomonas aeruginosa, and Achromobacter sp. were identified as efficient inulinase producers. The optimum temperature and pH of these organisms were found to be 40˚C and pH 5 respectively. Inulin was observed to be the suitable carbon source for these organisms. The molecular weight of this enzyme was estimated at 45 KDa using SDS-PAGE. On thin layer chromatography inulin hydrolysis showed mono and disaccharides as the main end products. The highest enzyme activity was obtained from Achromobacter spp. of 333 U/L at 22 nd hour which showed exo-inulinase type.
Microalgae lipid feedstock preparation cost was an important factor in increasing biodiesel fuel ... more Microalgae lipid feedstock preparation cost was an important factor in increasing biodiesel fuel hikes.This study was conducted
with the concept of implementing an effluent wastewater as lipid productionmediumformicroalgae cultivation. In our study textile
dyeing industry effluent was taken as a lipid production medium for Neochloris sp. cultivation. The changes in physicochemical
analysis of effluent before and after Neochloris sp. treatment were recorded using standard procedures and AAS analysis. There
was especially a reduction in heavymetal like lead (Pb) concentration from0.002ppm to 0.001ppm after Neochloris sp. treatment.
Neochloris sp. cultivated in Bold Basal Medium (BBM) (specific algal medium) produced 41.93% total lipid and 36.69% lipid was
produced in effluent based cultivation. Surprisingly Neochloris sp. cultivated in effluent was found with enhanced neutral lipid
content, and it was confirmed by Nile red fluorescence assay. Further the particular enrichment in oleic acid content of the cells
was confirmed with thin layer chromatography (TLC) with oleic acid pure (98%) control. The overall results suggested that textile
dyeing industry effluent could serve as the best lipid productive medium for Neochloris sp. biodiesel feedstock preparation. This study was found to have a significant impact on reducing the biodiesel feedstock preparation cost with simultaneous lipid induction by heavy metal stress to microalgae.
Microalgae are considered to be the world’s future energy reservoir and various aspects have been... more Microalgae are considered to be the world’s future energy reservoir and various aspects have
been followed for optimizing the algae growth for large scale production of biodiesel. For
the same, selection and screening of microalgae strain for its lipid productivity is much more
important. Fourier transform infrared (FTIR) spectroscopy was used in this study for the
analysis of lipid extract from natural isolate of Chlorella vulgaris. In order to evaluate the
strain productivity the reference strain was obtained. Both the cultures were grown in a
chemically-defined media under photoautotrophic culture conditions. Lipid extraction
procedures were standardized for both strains based on Fourier transform infrared (FTIR)
spectroscopy .The extracted lipid samples were analyzed. This study reveals that the natural
isolate identified as Chlorella vulgaris strain is suitable for further optimization procedures
and found with high percentage of residual lipid proportions. Also the results show that
FTIR technique can be applied for determination of single cell biomass composition from
phytoplankton communities.
BioMed research international, 2014
Microalgae lipid feedstock preparation cost was an important factor in increasing biodiesel fuel ... more Microalgae lipid feedstock preparation cost was an important factor in increasing biodiesel fuel hikes. This study was conducted with the concept of implementing an effluent wastewater as lipid production medium for microalgae cultivation. In our study textile dyeing industry effluent was taken as a lipid production medium for Neochloris sp. cultivation. The changes in physicochemical analysis of effluent before and after Neochloris sp. treatment were recorded using standard procedures and AAS analysis. There was especially a reduction in heavy metal like lead (Pb) concentration from 0.002 ppm to 0.001 ppm after Neochloris sp. treatment. Neochloris sp. cultivated in Bold Basal Medium (BBM) (specific algal medium) produced 41.93% total lipid and 36.69% lipid was produced in effluent based cultivation. Surprisingly Neochloris sp. cultivated in effluent was found with enhanced neutral lipid content, and it was confirmed by Nile red fluorescence assay. Further the particular enrichment ...
In the present study, microalgae were isolated from fresh pond water samples collected from Maman... more In the present study, microalgae were isolated from fresh pond water samples collected from Mamangam near Omalur Taluk, Salem. After performing the isolation procedure three microalgae isolates were successfully isolated .The synthetic medium namely B old Basal Medium (BBM), Tris acetate Phosphate (TAP), Bristol Medium were supplemented to all the three strains, further their biomass and lipid productivity are analysed. As a result one of the isolate namely Chlorella MS3 strain grown in Bristol medium found with higher percentage of total lipid. In particularly that Chlorella MS3 strain grown in Bristol was able to store neutral lipid proportion than other two strains. It was confirmed by Nile Red imaging studies. The Chlorella MS3 strain was selected for lipid enhancement procedures for biodiesel feed stock preparation.
Micro algae are photosynthetic miniatures capable of producing versatile compounds. It can provid... more Micro algae are photosynthetic miniatures capable of producing versatile compounds. It can provide biomass feed stock for different type of renewable fuels such as biodiesel, methane, hydrogen and ethanol. Among all, biodiesel can replace the present day fossil based fuel system. Other than bio-fuel production, microalgae can also serve for bioremediation and industry oriented processing. CO 2 sequestration from industrial flue gases by algae bio-fixation in turn reduces the green house gas emissions of an industry. Particularly algae biodiesel has no sulfur and more or less perform well than petroleum diesel. Emission gas contains reduced particulate matter, CO, hydrocarbon, SO 2. Even though it has enormous advantages, NO 2 emission from this diesel may be higher in some engine types. This review elaborates on the pros and cons of algal biodiesel usage in biodiesel sector.
Microbial inulinases have a great potential for industrial use in the production of fructose from... more Microbial inulinases have a great potential for industrial use in the production of fructose from inulin. Optimization of the growth parameters of the microbes is essential to obtain inulinase in sufficient quantity. The present work aimed to obtain inulinase from natural strains isolated from sugarcane fields and to optimize its growth parameters. In a total of ten isolates, four organisms Bacillus subtilis, Lactobacillus casei, Pseudomonas aeruginosa, and Achromobacter sp. were identified as efficient inulinase producers. The optimum temperature and pH of these organisms were found to be 40˚C and pH 5 respectively. Inulin was observed to be the suitable carbon source for these organisms. The molecular weight of this enzyme was estimated at 45 KDa using SDS-PAGE. On thin layer chromatography inulin hydrolysis showed mono and disaccharides as the main end products. The highest enzyme activity was obtained from Achromobacter spp. of 333 U/L at 22 nd hour which showed exo-inulinase type.
Microalgae lipid feedstock preparation cost was an important factor in increasing biodiesel fuel ... more Microalgae lipid feedstock preparation cost was an important factor in increasing biodiesel fuel hikes.This study was conducted
with the concept of implementing an effluent wastewater as lipid productionmediumformicroalgae cultivation. In our study textile
dyeing industry effluent was taken as a lipid production medium for Neochloris sp. cultivation. The changes in physicochemical
analysis of effluent before and after Neochloris sp. treatment were recorded using standard procedures and AAS analysis. There
was especially a reduction in heavymetal like lead (Pb) concentration from0.002ppm to 0.001ppm after Neochloris sp. treatment.
Neochloris sp. cultivated in Bold Basal Medium (BBM) (specific algal medium) produced 41.93% total lipid and 36.69% lipid was
produced in effluent based cultivation. Surprisingly Neochloris sp. cultivated in effluent was found with enhanced neutral lipid
content, and it was confirmed by Nile red fluorescence assay. Further the particular enrichment in oleic acid content of the cells
was confirmed with thin layer chromatography (TLC) with oleic acid pure (98%) control. The overall results suggested that textile
dyeing industry effluent could serve as the best lipid productive medium for Neochloris sp. biodiesel feedstock preparation. This study was found to have a significant impact on reducing the biodiesel feedstock preparation cost with simultaneous lipid induction by heavy metal stress to microalgae.
Microalgae are considered to be the world’s future energy reservoir and various aspects have been... more Microalgae are considered to be the world’s future energy reservoir and various aspects have
been followed for optimizing the algae growth for large scale production of biodiesel. For
the same, selection and screening of microalgae strain for its lipid productivity is much more
important. Fourier transform infrared (FTIR) spectroscopy was used in this study for the
analysis of lipid extract from natural isolate of Chlorella vulgaris. In order to evaluate the
strain productivity the reference strain was obtained. Both the cultures were grown in a
chemically-defined media under photoautotrophic culture conditions. Lipid extraction
procedures were standardized for both strains based on Fourier transform infrared (FTIR)
spectroscopy .The extracted lipid samples were analyzed. This study reveals that the natural
isolate identified as Chlorella vulgaris strain is suitable for further optimization procedures
and found with high percentage of residual lipid proportions. Also the results show that
FTIR technique can be applied for determination of single cell biomass composition from
phytoplankton communities.