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Papers by vitoria rodrigues
G3 Genes|Genomes|Genetics
The pathogenic yeast Candida glabrata is intrinsically resilient to azoles and rapidly acquires r... more The pathogenic yeast Candida glabrata is intrinsically resilient to azoles and rapidly acquires resistance to these antifungals, in vitro and in vivo. In most cases azole-resistant C. glabrata clinical strains encode hyperactive CgPdr1 variants, however, resistant strains encoding wild-type CgPDR1 alleles have also been isolated, although remaining to be disclosed the underlying resistance mechanism. In this study, we scrutinized the mechanisms underlying resistance to azoles of 8 resistant clinical C. glabrata strains, identified along the course of epidemiological surveys undertaken in Portugal. Seven of the strains were found to encode CgPdr1 gain-of-function variants (I392M, E555K, G558C, and I803T) with the substitutions I392M and I803T being herein characterized as hyper-activating mutations for the first time. While cells expressing the wild-type CgPDR1 allele required the mediator subunit Gal11A to enhance tolerance to fluconazole, this was dispensable for cells expressing t...
Herpetologia Brasileira, 2019
The H2H initiative promotes research and other initiatives that involve the participation of at l... more The H2H initiative promotes research and other initiatives that involve the participation of at least one Brazilian herpetologist and elaborates several social media posts with themes on environmental education and herpetofauna.
Access Microbiology, 2021
The relevance of C. glabrata as a human pathogen is linked with its poor susceptibility to azoles... more The relevance of C. glabrata as a human pathogen is linked with its poor susceptibility to azoles as well as its extreme genomic plasticity that allows the rapid acquisition of resistance. Extensive characterization of azole-resistant C. glabrata strains unveiled the central role of the transcriptional regulator CgPdr1 in the resistance phenotype, with many strains encoding hyperactive (or gain-of-function; GOF) CgPdr1 alleles. Large scale profiling of a collection of clinical C. glabrata isolates recovered in hospitals of the Lisbon area, in Portugal, led to the identification of 11 strains exhibiting resistance to fluconazole and voriconazole, while 2 were only resistant to fluconazole. Among these strains, 10 were found to encode alleles of the CgPDR1 gene harbouring multiple non-synonymous SNPs that were not found in the alleles encoded by susceptible strains, including K274Q, I392M and I803T not previously described as GOF mutations. The isolates encoding these alleles were fou...
In highly degraded areas, urban parks may have an important role in maintaining biodiversity. The... more In highly degraded areas, urban parks may have an important role in maintaining biodiversity. The Parque Estadual do Utinga, in the municipalities of Belem and Ananindeua, Para, Brazil, is in large part surrounded by human habitations, but it is connected to other forest fragments. We recently conducted surveys of the herpetofauna in this park and compared records from 1990 to the present with data from before 1985, in order to detect possible local extinctions due to the increase of urban and degraded areas. In total, 49 species of amphibians (45 frogs, one salamander, three caecilians) and 86 species of reptiles (27 lizards, four amphisbaenas, 49 snakes, one caiman, five turtles) were recorded in the park. Comparing the two periods, 16% of the amphibians and 11% of the lizards have not been found since 1990. We discuss the possibilities of local extinction versus sampling deficiency or other causes for the observed absences.
European Journal of Clinical Microbiology & Infectious Diseases, 2020
Neisseria gonorrhoeae antimicrobial resistance (AMR) and gonorrhea disease burden remain major pu... more Neisseria gonorrhoeae antimicrobial resistance (AMR) and gonorrhea disease burden remain major public health concerns worldwide. To contribute to the supranational demands to monitor and manage the spread of antimicrobial-resistant N. gonorrhoeae, the Portuguese NIH promoted the creation of the National Laboratory Network for Neisseria gonorrhoeae Collection (PTGonoNet). The present study reports the N. gonorrhoeae major AMR trends observed from 2003 up to 2018. All isolates described in the present study constitute the opportunistic ongoing N. gonorrhoeae isolate collection supported by the National Reference Laboratory for Sexually Transmitted Infections of the Portuguese NIH, enrolling strains isolated in 35 different public and private laboratories. Minimum inhibitory concentrations were determined using E-tests for azithromycin, benzylpenicillin, cefixime, ceftriaxone, ciprofloxacin, gentamicin, spectinomycin and tetracycline. Molecular typing was determined using NG-MAST. AMR data of 2596 country-spread isolates show that 87.67% of all N. gonorrhoeae isolates presented decreased susceptibility to at least one antimicrobial. A continuous decreased susceptibility and resistance to penicillin, tetracycline and ciprofloxacin can be observed along the years. However, no decreased susceptibility to cephalosporins was observed until 2018, while for azithromycin, this was always low. The most common observed NG-MAST genogroups were G1407, G7445, G225, G2, and G1034. This study evidences the advantages of a nationwide collection of isolates and of centralized AMR testing to respond to supranational (EURO-GASP) requirements while providing unprecedented data on AMR in the context of 15 years of surveillance.
Colloquium Exactarum, Vol.11 N.4, 2019
The environmental diagnosis of the tributaries of the Lajeado Stream tributaries in Pirapozinho /... more The environmental diagnosis of the tributaries of the Lajeado Stream tributaries in Pirapozinho / SP is a study that will evaluate the characteristics of the natural environment, as well as the interactions and interferences caused by anthropogenic activities in the environment and the impacts resulting from these actions. This characterization will be done through visual on-site surveys, laboratory analysis and literature searches that allow a better understanding of the behavior of a watershed in face of external factors. In this sense, we evaluate procedures such as soil use and occupation, water quality, environmental valuation, development of vegetative areas, soil qualification, among other aspects. Thus, the interpretation of the quality of the natural resources raised and their interference will support an assessment of which identified impacts are likely to be mitigated in order to employ the necessary recovery and improvement technologies in the area.
Revista Neurociências, 2015
RESUMO Objetivo. Comparar os efeitos do método Kabat com a estimulação elétrica funcional (FES), ... more RESUMO Objetivo. Comparar os efeitos do método Kabat com a estimulação elétrica funcional (FES), bem como a associação dos métodos na capacidade funcional de pacientes hemiparéticos. Método. Trata-se de uma série de casos com três pacientes hemiparéticos. Os participantes foram avaliados ao início e término do tratamento quanto à amplitude de movimento articular de tornozelo, constituintes da marcha e funcionalidade. Os pacientes foram divididos aleatoriamente em: (A) tratado com Kabat; (B) tratado com FES e Kabat; (C) tratado com FES. Todos os participantes realizaram o treino de marcha, totalizando 25 sessões de 50 minutos cada. Resultados. Observou-se uma melhora na ADM de dorsiflexão dos pacientes (A) e (B), melhora da funcionalidade em (B) e (C), aumento do passo e passada nos pacientes (B) e (C), aumento do tamanho da base em todos os pacientes e uma melhora da cadência e velocidade em (A) e (C). Conclusões. O paciente (B) foi o participante que apresentou resultados positivos para todos os quesitos, o que indica que a associação do método Kabat e o FES, pode ser mais eficiente para os pacientes com sequelas de AVC.
G3 Genes|Genomes|Genetics
The pathogenic yeast Candida glabrata is intrinsically resilient to azoles and rapidly acquires r... more The pathogenic yeast Candida glabrata is intrinsically resilient to azoles and rapidly acquires resistance to these antifungals, in vitro and in vivo. In most cases azole-resistant C. glabrata clinical strains encode hyperactive CgPdr1 variants, however, resistant strains encoding wild-type CgPDR1 alleles have also been isolated, although remaining to be disclosed the underlying resistance mechanism. In this study, we scrutinized the mechanisms underlying resistance to azoles of 8 resistant clinical C. glabrata strains, identified along the course of epidemiological surveys undertaken in Portugal. Seven of the strains were found to encode CgPdr1 gain-of-function variants (I392M, E555K, G558C, and I803T) with the substitutions I392M and I803T being herein characterized as hyper-activating mutations for the first time. While cells expressing the wild-type CgPDR1 allele required the mediator subunit Gal11A to enhance tolerance to fluconazole, this was dispensable for cells expressing t...
Herpetologia Brasileira, 2019
The H2H initiative promotes research and other initiatives that involve the participation of at l... more The H2H initiative promotes research and other initiatives that involve the participation of at least one Brazilian herpetologist and elaborates several social media posts with themes on environmental education and herpetofauna.
Access Microbiology, 2021
The relevance of C. glabrata as a human pathogen is linked with its poor susceptibility to azoles... more The relevance of C. glabrata as a human pathogen is linked with its poor susceptibility to azoles as well as its extreme genomic plasticity that allows the rapid acquisition of resistance. Extensive characterization of azole-resistant C. glabrata strains unveiled the central role of the transcriptional regulator CgPdr1 in the resistance phenotype, with many strains encoding hyperactive (or gain-of-function; GOF) CgPdr1 alleles. Large scale profiling of a collection of clinical C. glabrata isolates recovered in hospitals of the Lisbon area, in Portugal, led to the identification of 11 strains exhibiting resistance to fluconazole and voriconazole, while 2 were only resistant to fluconazole. Among these strains, 10 were found to encode alleles of the CgPDR1 gene harbouring multiple non-synonymous SNPs that were not found in the alleles encoded by susceptible strains, including K274Q, I392M and I803T not previously described as GOF mutations. The isolates encoding these alleles were fou...
In highly degraded areas, urban parks may have an important role in maintaining biodiversity. The... more In highly degraded areas, urban parks may have an important role in maintaining biodiversity. The Parque Estadual do Utinga, in the municipalities of Belem and Ananindeua, Para, Brazil, is in large part surrounded by human habitations, but it is connected to other forest fragments. We recently conducted surveys of the herpetofauna in this park and compared records from 1990 to the present with data from before 1985, in order to detect possible local extinctions due to the increase of urban and degraded areas. In total, 49 species of amphibians (45 frogs, one salamander, three caecilians) and 86 species of reptiles (27 lizards, four amphisbaenas, 49 snakes, one caiman, five turtles) were recorded in the park. Comparing the two periods, 16% of the amphibians and 11% of the lizards have not been found since 1990. We discuss the possibilities of local extinction versus sampling deficiency or other causes for the observed absences.
European Journal of Clinical Microbiology & Infectious Diseases, 2020
Neisseria gonorrhoeae antimicrobial resistance (AMR) and gonorrhea disease burden remain major pu... more Neisseria gonorrhoeae antimicrobial resistance (AMR) and gonorrhea disease burden remain major public health concerns worldwide. To contribute to the supranational demands to monitor and manage the spread of antimicrobial-resistant N. gonorrhoeae, the Portuguese NIH promoted the creation of the National Laboratory Network for Neisseria gonorrhoeae Collection (PTGonoNet). The present study reports the N. gonorrhoeae major AMR trends observed from 2003 up to 2018. All isolates described in the present study constitute the opportunistic ongoing N. gonorrhoeae isolate collection supported by the National Reference Laboratory for Sexually Transmitted Infections of the Portuguese NIH, enrolling strains isolated in 35 different public and private laboratories. Minimum inhibitory concentrations were determined using E-tests for azithromycin, benzylpenicillin, cefixime, ceftriaxone, ciprofloxacin, gentamicin, spectinomycin and tetracycline. Molecular typing was determined using NG-MAST. AMR data of 2596 country-spread isolates show that 87.67% of all N. gonorrhoeae isolates presented decreased susceptibility to at least one antimicrobial. A continuous decreased susceptibility and resistance to penicillin, tetracycline and ciprofloxacin can be observed along the years. However, no decreased susceptibility to cephalosporins was observed until 2018, while for azithromycin, this was always low. The most common observed NG-MAST genogroups were G1407, G7445, G225, G2, and G1034. This study evidences the advantages of a nationwide collection of isolates and of centralized AMR testing to respond to supranational (EURO-GASP) requirements while providing unprecedented data on AMR in the context of 15 years of surveillance.
Colloquium Exactarum, Vol.11 N.4, 2019
The environmental diagnosis of the tributaries of the Lajeado Stream tributaries in Pirapozinho /... more The environmental diagnosis of the tributaries of the Lajeado Stream tributaries in Pirapozinho / SP is a study that will evaluate the characteristics of the natural environment, as well as the interactions and interferences caused by anthropogenic activities in the environment and the impacts resulting from these actions. This characterization will be done through visual on-site surveys, laboratory analysis and literature searches that allow a better understanding of the behavior of a watershed in face of external factors. In this sense, we evaluate procedures such as soil use and occupation, water quality, environmental valuation, development of vegetative areas, soil qualification, among other aspects. Thus, the interpretation of the quality of the natural resources raised and their interference will support an assessment of which identified impacts are likely to be mitigated in order to employ the necessary recovery and improvement technologies in the area.
Revista Neurociências, 2015
RESUMO Objetivo. Comparar os efeitos do método Kabat com a estimulação elétrica funcional (FES), ... more RESUMO Objetivo. Comparar os efeitos do método Kabat com a estimulação elétrica funcional (FES), bem como a associação dos métodos na capacidade funcional de pacientes hemiparéticos. Método. Trata-se de uma série de casos com três pacientes hemiparéticos. Os participantes foram avaliados ao início e término do tratamento quanto à amplitude de movimento articular de tornozelo, constituintes da marcha e funcionalidade. Os pacientes foram divididos aleatoriamente em: (A) tratado com Kabat; (B) tratado com FES e Kabat; (C) tratado com FES. Todos os participantes realizaram o treino de marcha, totalizando 25 sessões de 50 minutos cada. Resultados. Observou-se uma melhora na ADM de dorsiflexão dos pacientes (A) e (B), melhora da funcionalidade em (B) e (C), aumento do passo e passada nos pacientes (B) e (C), aumento do tamanho da base em todos os pacientes e uma melhora da cadência e velocidade em (A) e (C). Conclusões. O paciente (B) foi o participante que apresentou resultados positivos para todos os quesitos, o que indica que a associação do método Kabat e o FES, pode ser mais eficiente para os pacientes com sequelas de AVC.