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Monolithic columns are continuous interconnected networks with large through-pore channels. This ... more Monolithic columns are continuous interconnected networks with large through-pore channels. This structure results in a decrease in the diffusion path and affords high permeability, which result in obtaining good separation efficiency. Ideally, the structure of monoliths should be bi-model consisting of meso-pores and macro-pores responsible for retention time and flow of mobile phase respectively. The structure also enhances the mechanical strength, and the large through-pore channels afford very low flow resistance. This combination therefore results in the ability of smaller diameter monolithic columns to be employed under high flow rates, increasing both sensitivity and throughput simultaneously. Additionally, the unique structure of monoliths improves permeability and mass transfer leading to a decrease in band broadening. The first stage of the project was focused on the fabrication and characterisation of an organic monolithic column namely poly (SMA-co-EDMA) followed by quantification of caffeine in Arabic coffee. Since the efficiency of the above monolith was low due to the low number of mesopores (low surface area), the second stage was centered on improving the efficiency of
Monolithic columns are continuous interconnected networks with large through-pore channels. This ... more Monolithic columns are continuous interconnected networks with large through-pore channels. This structure results in a decrease in the diffusion path and affords high permeability, which result in obtaining good separation efficiency. Ideally, the structure of monoliths should be bi-model consisting of meso-pores and macro-pores responsible for retention time and flow of mobile phase respectively. The structure also enhances the mechanical strength, and the large through-pore channels afford very low flow resistance. This combination therefore results in the ability of smaller diameter monolithic columns to be employed under high flow rates, increasing both sensitivity and throughput simultaneously. Additionally, the unique structure of monoliths improves permeability and mass transfer leading to a decrease in band broadening. The first stage of the project was focused on the fabrication and characterisation of an organic monolithic column namely poly (SMA-co-EDMA) followed by quantification of caffeine in Arabic coffee. Since the efficiency of the above monolith was low due to the low number of mesopores (low surface area), the second stage was centered on improving the efficiency of
IOSR Journal of Biotechnology and Biochemistry, 2017
Tissue engineering and 3D cell cutler involving the use of three-dimensional scahffold for cell a... more Tissue engineering and 3D cell cutler involving the use of three-dimensional scahffold for cell and tissue cultural ,is a promising technique for establishing in vitro culture models that mimc in vivo environment. In vitro models present ethical and cost advantages over in vivo models, and allow for tissue devolpmint.alginate , aliner polysaccharide derived from brown algae .has properties that make it a favorable materials as a 3D extracellular matrix for in vitro cell and tissue models and some of sodium Hydrogyl materials are higly appealing as 3D scaffold for in vitro 3D cell cutlre and tissue culture due their ability to mimic the physical properties native tissue as well as their high porosity for effcient diffusion of proteins and nutrients Alginate hydrogel is widely ysed in tissue engineering application due to its desirable propertis as Biomaterials .
Monolithic columns are continuous interconnected networks with large through-pore channels. This ... more Monolithic columns are continuous interconnected networks with large through-pore channels. This structure results in a decrease in the diffusion path and affords high permeability, which result in obtaining good separation efficiency. Ideally, the structure of monoliths should be bi-model consisting of meso-pores and macro-pores responsible for retention time and flow of mobile phase respectively. The structure also enhances the mechanical strength, and the large through-pore channels afford very low flow resistance. This combination therefore results in the ability of smaller diameter monolithic columns to be employed under high flow rates, increasing both sensitivity and throughput simultaneously. Additionally, the unique structure of monoliths improves permeability and mass transfer leading to a decrease in band broadening. The first stage of the project was focused on the fabrication and characterisation of an organic monolithic column namely poly (SMA-co-EDMA) followed by quantification of caffeine in Arabic coffee. Since the efficiency of the above monolith was low due to the low number of mesopores (low surface area), the second stage was centered on improving the efficiency of
Monolithic columns are continuous interconnected networks with large through-pore channels. This ... more Monolithic columns are continuous interconnected networks with large through-pore channels. This structure results in a decrease in the diffusion path and affords high permeability, which result in obtaining good separation efficiency. Ideally, the structure of monoliths should be bi-model consisting of meso-pores and macro-pores responsible for retention time and flow of mobile phase respectively. The structure also enhances the mechanical strength, and the large through-pore channels afford very low flow resistance. This combination therefore results in the ability of smaller diameter monolithic columns to be employed under high flow rates, increasing both sensitivity and throughput simultaneously. Additionally, the unique structure of monoliths improves permeability and mass transfer leading to a decrease in band broadening. The first stage of the project was focused on the fabrication and characterisation of an organic monolithic column namely poly (SMA-co-EDMA) followed by quantification of caffeine in Arabic coffee. Since the efficiency of the above monolith was low due to the low number of mesopores (low surface area), the second stage was centered on improving the efficiency of
IOSR Journal of Biotechnology and Biochemistry, 2017
Tissue engineering and 3D cell cutler involving the use of three-dimensional scahffold for cell a... more Tissue engineering and 3D cell cutler involving the use of three-dimensional scahffold for cell and tissue cultural ,is a promising technique for establishing in vitro culture models that mimc in vivo environment. In vitro models present ethical and cost advantages over in vivo models, and allow for tissue devolpmint.alginate , aliner polysaccharide derived from brown algae .has properties that make it a favorable materials as a 3D extracellular matrix for in vitro cell and tissue models and some of sodium Hydrogyl materials are higly appealing as 3D scaffold for in vitro 3D cell cutlre and tissue culture due their ability to mimic the physical properties native tissue as well as their high porosity for effcient diffusion of proteins and nutrients Alginate hydrogel is widely ysed in tissue engineering application due to its desirable propertis as Biomaterials .