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Papers by willa wang

Research paper thumbnail of Exploratory Investigation of the Plasma Proteome Associated with the Endotheliopathy of Trauma

International Journal of Molecular Sciences

Background: The endotheliopathy of trauma (EoT) is associated with increased mortality following ... more Background: The endotheliopathy of trauma (EoT) is associated with increased mortality following injury. Herein, we describe the plasma proteome related to EoT in order to provide insight into the role of the endothelium within the systemic response to trauma. Methods: 99 subjects requiring the highest level of trauma activation were included in the study. Enzyme-linked immunosorbent assays of endothelial and catecholamine biomarkers were performed on admission plasma samples, as well as untargeted proteome quantification utilizing high-performance liquid chromatography and tandem mass spectrometry. Results: Plasma endothelial and catecholamine biomarker abundance was elevated in EoT. Patients with EoT (n = 62) had an increased incidence of death within 24 h at 21% compared to 3% for non-EoT (n = 37). Proteomic analysis revealed that 52 out of 290 proteins were differentially expressed between the EoT and non-EoT groups. These proteins are involved in endothelial activation, coagula...

Research paper thumbnail of The Effects on Thrombin Generation Following Resuscitation with Lactated Ringers and Fresh Frozen Plasma

Research paper thumbnail of Corn Producer Practices and Insect Resistance Management Requirements

We document the degree to which corn producers in South Dakota follow Insect Resistance Managemen... more We document the degree to which corn producers in South Dakota follow Insect Resistance Management requirements administered by the Environmental Protection Agency. Our findings are based a survey held among a sample of 317 corn producers in South Dakota in July of 2004. Detailed results are forthcoming.

Research paper thumbnail of Abstract 355: Autologous Cleavage of the GPIbalpha Receptor Promotes Platelet Dysfunction Following Trauma

Research paper thumbnail of Elevated Syndecan-1 after Trauma and Risk of Sepsis: A Secondary Analysis of Patients from the Pragmatic, Randomized Optimal Platelet and Plasma Ratios (PROPPR) Trial

Journal of the American College of Surgeons, 2018

Background: Endotheliopathy of trauma is characterized by breakdown of the endothelial glycocalyx... more Background: Endotheliopathy of trauma is characterized by breakdown of the endothelial glycocalyx. Elevated biomarkers of endotheliopathy, such as serum syndecan-1 (Synd-1) ≥ 40 ng/mL, have been associated with increased need for transfusions, complications, and mortality. We hypothesized that severely injured trauma patients who exhibit elevated Synd-1 levels shortly after admission have an increased likelihood of developing sepsis. Study Design: We analyzed a subset of PROPPR patients that survived at least 72 hours after hospital admission and determined elevated Synd-1 levels (≥ 40 ng/mL) 4 hours after hospital arrival. Sepsis was defined a priori as meeting systemic inflammatory response criteria and having a known or suspected infection. Univariate analysis was performed to identify variables associated with elevated Synd-1 levels and sepsis. Significant variables at a p-value <0.2 in the univariate analysis were chosen by purposeful selection and analyzed in a mixed effects multivariate logistic regression model to account for the 12 different study sites. Results: We included 512 patients. Of these, 402 (79%) had elevated Synd-1 levels, and 180 (35%) developed sepsis. Median Synd-1 levels at 4 hours after admission were 70 ng/dL (IQR 36-157 ng/dL) in patients who did not develop sepsis, and 165 ng/dL (IQR 67-336 ng/dL) in those who did (p < 0.001). Adjusting for treatment arm and site, multivariable analyses revealed that

Research paper thumbnail of Onset of Coagulation Function Recovery Is Delayed in Severely Injured Trauma Patients with Venous Thromboembolism

Journal of the American College of Surgeons, Jan 15, 2017

Altered coagulation function after trauma may contribute to venous thromboembolism (VTE) developm... more Altered coagulation function after trauma may contribute to venous thromboembolism (VTE) development. Severe trauma impairs coagulation function, but the trajectory for recovery is not known. We hypothesized that enhanced, early recovery of coagulation function increases VTE risk in severely-injured trauma patients. Secondary analysis was performed on data from The Pragmatic Randomized Optimal Platelet and Plasma Ratio (PROPPR) trial, excluding patients who died within 24 hours and/or were on pre-injury anticoagulants. Patient characteristics, adverse outcomes, and parameters of platelet function (PF) and coagulation (thromboelastography; TEG) were compared from admission to 72 hours between VTE (n=83) and non-VTE (n=475) patients. p<0.05 indicated significance. Despite similar patient demographics, VTE patients exhibited hypercoagulable TEG parameters and enhanced PF at admission (p<0.05). Both groups exhibited hypocoagulable TEG parameters, platelet dysfunction and suppresse...

Research paper thumbnail of Abstract 355: Autologous Cleavage of the GPIbalpha Receptor Promotes Platelet Dysfunction Following Trauma

Circulation, Nov 26, 2013

Research paper thumbnail of Response of acoustic and elastic properties to pressure and crystallization of Ce-based bulk metallic glass

Physical Review B, 2005

The density and acoustic velocities of a Ce 70 Al 10 Ni 10 Cu 10 bulk metallic glass ͑BMG͒ under ... more The density and acoustic velocities of a Ce 70 Al 10 Ni 10 Cu 10 bulk metallic glass ͑BMG͒ under hydrostaticpressure ͑up to 0.5 GPa͒ and in crystallized state in ambient conditions were measured in situ by a pulse echo overlap method. The pressure derivatives of velocities and Grüneisen parameters as well as the equation of state ͑EOS͒ of the BMG were determined and compared to those of various other BMGs and nonmetallic glasses. Surprisingly, the BMG, unlike other BMGs with normal mode stiffness, exhibits an anomalous soft longitudinal acoustic mode under pressure similar to that of typical oxide glasses. An unusually large softening of longitudinal acoustic phonons in the BMG, relative to its crystalline state, is also observed, analogous with that in oxide glasses. The possible origin for the anomaly is the intrinsic glassy structure containing short-range covalent bonds.

Research paper thumbnail of Exploratory Investigation of the Plasma Proteome Associated with the Endotheliopathy of Trauma

International Journal of Molecular Sciences

Background: The endotheliopathy of trauma (EoT) is associated with increased mortality following ... more Background: The endotheliopathy of trauma (EoT) is associated with increased mortality following injury. Herein, we describe the plasma proteome related to EoT in order to provide insight into the role of the endothelium within the systemic response to trauma. Methods: 99 subjects requiring the highest level of trauma activation were included in the study. Enzyme-linked immunosorbent assays of endothelial and catecholamine biomarkers were performed on admission plasma samples, as well as untargeted proteome quantification utilizing high-performance liquid chromatography and tandem mass spectrometry. Results: Plasma endothelial and catecholamine biomarker abundance was elevated in EoT. Patients with EoT (n = 62) had an increased incidence of death within 24 h at 21% compared to 3% for non-EoT (n = 37). Proteomic analysis revealed that 52 out of 290 proteins were differentially expressed between the EoT and non-EoT groups. These proteins are involved in endothelial activation, coagula...

Research paper thumbnail of The Effects on Thrombin Generation Following Resuscitation with Lactated Ringers and Fresh Frozen Plasma

Research paper thumbnail of Corn Producer Practices and Insect Resistance Management Requirements

We document the degree to which corn producers in South Dakota follow Insect Resistance Managemen... more We document the degree to which corn producers in South Dakota follow Insect Resistance Management requirements administered by the Environmental Protection Agency. Our findings are based a survey held among a sample of 317 corn producers in South Dakota in July of 2004. Detailed results are forthcoming.

Research paper thumbnail of Abstract 355: Autologous Cleavage of the GPIbalpha Receptor Promotes Platelet Dysfunction Following Trauma

Research paper thumbnail of Elevated Syndecan-1 after Trauma and Risk of Sepsis: A Secondary Analysis of Patients from the Pragmatic, Randomized Optimal Platelet and Plasma Ratios (PROPPR) Trial

Journal of the American College of Surgeons, 2018

Background: Endotheliopathy of trauma is characterized by breakdown of the endothelial glycocalyx... more Background: Endotheliopathy of trauma is characterized by breakdown of the endothelial glycocalyx. Elevated biomarkers of endotheliopathy, such as serum syndecan-1 (Synd-1) ≥ 40 ng/mL, have been associated with increased need for transfusions, complications, and mortality. We hypothesized that severely injured trauma patients who exhibit elevated Synd-1 levels shortly after admission have an increased likelihood of developing sepsis. Study Design: We analyzed a subset of PROPPR patients that survived at least 72 hours after hospital admission and determined elevated Synd-1 levels (≥ 40 ng/mL) 4 hours after hospital arrival. Sepsis was defined a priori as meeting systemic inflammatory response criteria and having a known or suspected infection. Univariate analysis was performed to identify variables associated with elevated Synd-1 levels and sepsis. Significant variables at a p-value <0.2 in the univariate analysis were chosen by purposeful selection and analyzed in a mixed effects multivariate logistic regression model to account for the 12 different study sites. Results: We included 512 patients. Of these, 402 (79%) had elevated Synd-1 levels, and 180 (35%) developed sepsis. Median Synd-1 levels at 4 hours after admission were 70 ng/dL (IQR 36-157 ng/dL) in patients who did not develop sepsis, and 165 ng/dL (IQR 67-336 ng/dL) in those who did (p < 0.001). Adjusting for treatment arm and site, multivariable analyses revealed that

Research paper thumbnail of Onset of Coagulation Function Recovery Is Delayed in Severely Injured Trauma Patients with Venous Thromboembolism

Journal of the American College of Surgeons, Jan 15, 2017

Altered coagulation function after trauma may contribute to venous thromboembolism (VTE) developm... more Altered coagulation function after trauma may contribute to venous thromboembolism (VTE) development. Severe trauma impairs coagulation function, but the trajectory for recovery is not known. We hypothesized that enhanced, early recovery of coagulation function increases VTE risk in severely-injured trauma patients. Secondary analysis was performed on data from The Pragmatic Randomized Optimal Platelet and Plasma Ratio (PROPPR) trial, excluding patients who died within 24 hours and/or were on pre-injury anticoagulants. Patient characteristics, adverse outcomes, and parameters of platelet function (PF) and coagulation (thromboelastography; TEG) were compared from admission to 72 hours between VTE (n=83) and non-VTE (n=475) patients. p<0.05 indicated significance. Despite similar patient demographics, VTE patients exhibited hypercoagulable TEG parameters and enhanced PF at admission (p<0.05). Both groups exhibited hypocoagulable TEG parameters, platelet dysfunction and suppresse...

Research paper thumbnail of Abstract 355: Autologous Cleavage of the GPIbalpha Receptor Promotes Platelet Dysfunction Following Trauma

Circulation, Nov 26, 2013

Research paper thumbnail of Response of acoustic and elastic properties to pressure and crystallization of Ce-based bulk metallic glass

Physical Review B, 2005

The density and acoustic velocities of a Ce 70 Al 10 Ni 10 Cu 10 bulk metallic glass ͑BMG͒ under ... more The density and acoustic velocities of a Ce 70 Al 10 Ni 10 Cu 10 bulk metallic glass ͑BMG͒ under hydrostaticpressure ͑up to 0.5 GPa͒ and in crystallized state in ambient conditions were measured in situ by a pulse echo overlap method. The pressure derivatives of velocities and Grüneisen parameters as well as the equation of state ͑EOS͒ of the BMG were determined and compared to those of various other BMGs and nonmetallic glasses. Surprisingly, the BMG, unlike other BMGs with normal mode stiffness, exhibits an anomalous soft longitudinal acoustic mode under pressure similar to that of typical oxide glasses. An unusually large softening of longitudinal acoustic phonons in the BMG, relative to its crystalline state, is also observed, analogous with that in oxide glasses. The possible origin for the anomaly is the intrinsic glassy structure containing short-range covalent bonds.