yang wen - Academia.edu (original) (raw)
Papers by yang wen
Water
Ecological engineering construction is the main method for urban riparian landscape restoration. ... more Ecological engineering construction is the main method for urban riparian landscape restoration. Continuous ecological environmental monitoring can reflect the effects of engineering construction and can provide a scientific basis for the improvement of engineering technology. However, the evaluation of riparian ecological engineering mainly focuses on the water environmental function and biodiversity conservation function after the construction period. Studies on the long-term evaluation of regional microclimate regulation and human settlement improvement are limited. In this paper, an ecological restoration project along the Yitong River in northern China was selected as the research object. Temperature, relative humidity and wind speed under different vegetation type combinations were monitored in the riparian ecological engineering construction during the first, second, third and tenth years. The temperature–humidity index (THI) was selected to evaluate human comfort and the imp...
Based on the survey of 154 farmers in Guiyang City, we analyze the basic situation of building of... more Based on the survey of 154 farmers in Guiyang City, we analyze the basic situation of building of grass-roots agricultural technology extension system in Guiyang City. Then we point out some problems in the building of grass-roots agricultural technology extension system in Guiyang City: the function of grass-roots agricultural technology extension center weakens; the content of agricultural technology extension is difficult to adapt to farmers' needs for technology; the extension mode of agricultural technology departments does not adapt to the needs of modern agriculture. In order to perfect the building of grass-roots agricultural technology extension system, the countermeasures and recommendations are put forth as follows: strengthening the input of funds, and ensuring that the basic work of public welfare agricultural technology extension is smoothly carried out; innovating upon the system, and improving the function of grass-roots agricultural technology extension center; ...
IEEE Transactions on Vehicular Technology, 2020
Advances in Building Technology, 2002
Publisher Summary This chapter proposes a new method of three-dimensional (3D) modeling based on ... more Publisher Summary This chapter proposes a new method of three-dimensional (3D) modeling based on the virtual reality (VR) and traditional CAD modeling technologies. World tool kit (WTK) is selected as the platform for VR environment. It is an advanced cross-platform development environment for high-performance, realtime 3D graphics applications. With the high-level application programmer's interface (API), end-users of WTK can write codes to call the functions in WTK function library, and then create the virtual world. To add and modify data of CAD models in a VR environment, a two-way data transformer between VR and CAD environment should be implemented. When the models are modified on AutoCAD, corresponding data in the VR environment should be automatically updated, and vice versa. Since the modeling functions of AutoCAD will be directly utilized, making changes to the structural models under AutoCAD will be convenient. In order to display the 3D models in a VR environment, data of models generated from AutoCAD must be translated into one of the formats that WTK can process. That is, after constructing the models under AutoCAD, users should save them in a DXF format file at first, and then use the WUM to open this DXF file, and make further conversion or map textures.
International Journal of Economics and Finance, 2020
Researchers have not come to an agreement on the impact of political connection on enterprise per... more Researchers have not come to an agreement on the impact of political connection on enterprise performance although this issue draws much attention. This paper attributes the above phenomenon to lack of precise classification of various political connection types in China. Based on existing literature, this paper divides political connections into current political connections and former political connections, and identifies their own hierarchy. Empirical study using Chinese Private Enterprise Survey data shows that different sorts of political connections all contribute to enterprise performance, and internal governance plays the mediating role. Overall, this paper may make marginal contributions to the study on the relationship between political connections and enterprise performance.
The purpose of this research is to address the problems of urban-rural dual economic structure, p... more The purpose of this research is to address the problems of urban-rural dual economic structure, promote the integr ated urban and rural development, and explore the difficulties of economic development in ethnic and border areas. Methods including principal components analysis are deployed to conduct comprehensive appraisal of regional ec onomic and social development, and in-depth analysis of development level, vitality, potential, characteristic, type and/or mode of towns in ethnic and border areas of Yunnan province. The development disparities, causes and prob lems in relation to towns in ethnic and border areas of Yunnan are summarized and analyzed. Policy implications a nd suggestions are proposed to develop small towns in the process of new rural construction and building a compre hensive well-off society in ethnic and border areas. Biography of Yang Wen As a senior statistician and associate professor, he got his bachelor in 1984 and master’s degree in 1991 from Yunnan Unive...
Leprosy Review, 2009
To evaluate the reliability and feasibility of two methods of multilocus variable number of tande... more To evaluate the reliability and feasibility of two methods of multilocus variable number of tandem repeat analysis (MLVA) for strain typing of M. leprae, and to study whether short tandem repeat loci are stable and suitable for epidemiological study of leprosy. Total DNA was extracted from skin biopsies of 20 new multibacillary (MB) patients from China diagnosed in 2006. To determine the copy numbers of short tandem repeats (STRs) for 13 loci, we amplified each locus individually by PCR, followed by sequence analysis of the amplicons. Separately, the same loci, plus four others were amplified by Multiplex PCRs (MP) using fluorescent primers and the copy number was identified by fragment length analysis (MP-FLA). MLVA was also performed at different times during treatment for a subset of the patients. Genetic variability of M. leprae in China can be assessed in microsatellite loci. (GTA)9 and (TTC)21 loci are hypervariable, with array sizes of 25 repeat units or more. The expansion of the (GTA)9 locus is a characteristic of some M. leprae isolates in China. A high level of allele concordance was observed between PCR-sequencing and MP-FLA methods. However, MP-FLA method was cost-effective, rapid, high throughput and suitable for strain typing. Five of the 20 isolates of M. leprae were from patients residing in the same township in Qiubei County, Yunnan, and matched closely by MLVA. Three of these patients are family contacts of previously diagnosed patients, with intra-familial strain types being similar, suggesting infections from common sources and transmission chain(s). The VNTR patterns were highly similar in biopsy and slit skin smears (SSS) before treatment, and in the SSS collected at various time points during treatment. Taken together, VNTR strain typing is a useful tool for study of short range transmission in leprosy.
2019 34th IEEE/ACM International Conference on Automated Software Engineering (ASE), 2019
We present PTracer, a Linux kernel patch trace bot based on an improved PatchNet. PTracer continu... more We present PTracer, a Linux kernel patch trace bot based on an improved PatchNet. PTracer continuously monitors new patches in the git repository of the mainline Linux kernel, filters out unconcerned ones, classifies the rest as bug-fixing or non bug-fixing patches, and reports bug-fixing patches to the kernel experts of commercial operating systems. We use the patches in February 2019 of the mainline Linux kernel to perform the test. As a result, PTracer recommended 151 patches to CGEL kernel experts out of 5,142, and 102 of which were accepted. PTracer has been successfully applied to a commercial operating system and has the advantages of improving software quality and saving labor cost.
2021 IEEE/ACM 13th International Workshop on Cooperative and Human Aspects of Software Engineering (CHASE), 2021
International Journal of Economics and Finance, 2020
Based on data of Chinese Private Enterprises Survey (CPES) from 2006-2014, this paper uses OLS mo... more Based on data of Chinese Private Enterprises Survey (CPES) from 2006-2014, this paper uses OLS model and other empirical methods to estimate the impacts of political connections on private enterprise performance in China, as well as heterogeneous effects and mediating effects of different types of political connections on tax burden and non-productive activities expense. The results show that, political connections contribute significantly to private enterprises. Compared with previous political connections, like working experiences in state-owned enterprises and government-affiliated institutions, current political connections, like deputies to the NPC or members of CPPCC, have played better and further roles in enterprise performance. Tax burden and non-productive activities expense have a mediation effect in the relationship between political connections and enterprise performance. This study replenishes new evidence to describe how political connections affect private enterprise...
Water, 2020
Small- and medium-sized rivers are facing a serious degradation of ecological function in water r... more Small- and medium-sized rivers are facing a serious degradation of ecological function in water resource-scarce regions of Northern China. Reservoir ecological operation can restore the damaged river ecological environment. Research on reservoir ecological operation and watershed management of urban rivers is limited in cold regions of middle and high latitudes. In this paper, the urban section of the Yitong River was selected as the research object in Changchun, Northern China. The total ecological water demand and reservoir operation water (79.35 × 106 m3 and 15.52 × 106 m3, respectively) were calculated by the ecological water demand method, and a reservoir operation scheme was established to restore the ecological function of the urban section of the river. To examine the scientific basis and rationality of the operation scheme, the water quality of the river and physical habitat after carrying out the scheme were simulated by the MIKE 11 one-dimensional hydrodynamic-water quali...
ECS Meeting Abstracts, 2014
Metabolites, 2019
Copper (Cu) is widely used in the swine industry to improve the growth performance of pigs. Howev... more Copper (Cu) is widely used in the swine industry to improve the growth performance of pigs. However, high doses of copper will induce cell damage and toxicity. The aim of this study was to evaluate toxicity, bioavailability, and effects on metabolic processes of varying copper sources using porcine intestinal epithelial cells (IPEC-J2) as a model. The IPEC-J2 were treated with two doses (30 and 120 μM) of CuSO4, Cu Glycine (Cu-Gly), and Cu proteinate (Cu-Pro) for 10 h, respectively. Cell damage and cellular copper metabolism were measured by the changes in cell viability, copper uptake, oxidative stress biomarkers, and gene/protein expression levels. The results showed that cell viability and ratio of reduced and oxidized glutathione (GSH/GSSG) decreased significantly in all treatment groups; intracellular copper content increased significantly in all treatment groups; total superoxide dismutase (SOD) activity increased significantly in the 120 μM exposed groups; SOD1 protein expres...
International Journal of Agricultural and Biological Engineering, 2017
Fast pyrolysis of corn stover was performed at temperatures in the range of 450°C-525°C in a flui... more Fast pyrolysis of corn stover was performed at temperatures in the range of 450°C-525°C in a fluidized bed. The chemical composition of the bio-oil acquired was analyzed by GC/MS, and its pH, kinetic viscosity and calorific values were determined. In the pyrolysis system used in this experiment, some improvements to former pyrolysis systems were done. Two screw feeders were used to prevent jamming the feeding system, and the condenser was equipped with some nozzles and a heat exchanger to cool quickly the cleaned hot gas into bio-oil. The results showed that the bio-oil yield increased with increasing pyrolysis temperature and then declined with a further increase in pyrolysis temperature. The highest bio-oil yield of 43.3 wt.% was obtained at 500°C with the dolomite as bed material. The char yield always decreased with the increase of temperature. The major chemical compounds of bio-oil included acetol, butanone, acetic acid, propionic acid, ethylene glycol, phenol, etc. Pyrolysis oil was completely immiscible in diesel, but homogeneous emulsions were obtained when mixing the pyrolysis oil, emulsifier with diesel in different ratios.
Dental materials journal, Jan 14, 2018
The objective of this study was to investigate the effects of a silicate-based composite material... more The objective of this study was to investigate the effects of a silicate-based composite material on proliferation and mineralization of human dental pulp cells (hDPCs), which was compared with those of calcium hydroxide (Ca(OH), CH) and tricalcium silicate (CaSiO, C3S). HDPCs were cultured with CH, C3S and tricalcium silicate/dicalcium silicate (CaSiO/CaSiO, C3S/C2S) composites extract. The CCK-8 assay showed that the composite material stimulated the proliferation of hDPCs. The odontogenic marker genes and DSPP protein expression were more significantly up-regulated by the C3S/C2S composite material compared with pure CH and C3S. HDPCs cultured with composite material extract exert stronger ALP activity and alizarin red S staining. C3S/C2S composite material was advantageous over pure C3S by showing enhanced ability to stimulate the proliferation and odontogenic differentiation of hDPCs, suggesting that the C3S/C2S composite materials possess desirable biocompatibility and bioacti...
Water science and technology : a journal of the International Association on Water Pollution Research, 2018
Anaerobic digestion with thermal hydrolysis pretreatment (THP), also called advanced anaerobic di... more Anaerobic digestion with thermal hydrolysis pretreatment (THP), also called advanced anaerobic digestion (AAD), is a mainstream technology for sludge treatment. AAD changes sludge, it can degrade extracellular polymeric substances (EPS), release EPS from the sludge, and alter the particle size distribution. We synthesized PFS-PDMDAAC from the inorganic coagulant polyferric sulfate (PFS) and the organic coagulant polymer polydimethyldiallylammonium chloride (PDMDAAC) in various PFS:PDMDAAC weight ratios. We investigated the effects of PFS-PDMDAAC pretreatment on AAD sludge dewaterability, and developed an explanation for them. Capillary suction time (CST) was used as a measure of sludge dewaterability. Dissolved organic matter, the three-dimensional excitation emission matrix, particle size (d), zeta potential, and sludge microstructure were observed in order to explain changes in sludge dewaterability that resulted from different compositions and dosages of coagulants. Treatment wit...
Shanghai kou qiang yi xue = Shanghai journal of stomatology, 2017
To construct an expression vector of a small hairpin RNA (shRNA) targeting human PERK gene and to... more To construct an expression vector of a small hairpin RNA (shRNA) targeting human PERK gene and to observe gene-silencing effects of PERK in human dental pulp cells (DPCs). According to PERK gene cDNA sequence, shRNA was designed and synthesized, which was then annealed into hU6-MCS-CMV-EGFP vector. After identified by sequencing, hU6-MCS-CMV-EGFP vector and packaging vector were co-transfected into 293 T cells. 72 hours later, the recombinant lentiviruses were obtained after harvesting and concentrating. Then LV-PERK-RNAi vectors were transfected into DPCs at an appropriate multiplicity of infection. To verify the interference effect, real- time PCR and Western blot were used to detect expression levels of PERK mRNA and protein in the transfected DPCs. The data were analyzed with SPSS 24.0 software package. LV-PERK-RNAi vectors were successfully constructed with a high titer of 3×10 TU/mL. The results of RT-PCR and Western blot demonstrated that after infection with LV-PERK-RNAi vec...
Experimental and therapeutic medicine, 2018
The objective of the present study was to examine whether supplementation has a role in the regul... more The objective of the present study was to examine whether supplementation has a role in the regulation of the intestinal flora and the development of the immune system of neonatal mice. A total of 30 pregnant BALB/c mice, including their offspring, were randomly divided into three groups: In the maternal intervention group (Ba), maternal mice were treated with from birth until weaning at postnatal day 21 (PD21) followed by administration of saline to the offspring at PD21-28; in the offspring intervention group (Ab), breast-feeding maternal mice were supplemented with saline and offspring were directly supplemented with from PD21-28; in the both maternal and offspring intervention group (Bb), both maternal mice and offspring were supplemented with at PD 0-21 and at PD21-28. While mice in the control group were given the same volume of normal saline. Stool samples from the offspring were collected at PD14, -21 and -28 to observe the intestinal flora by colony counts of spp., spp., sp...
Advanced materials (Deerfield Beach, Fla.), 2018
Solid-state batteries have many enticing advantages in terms of safety and stability, but the sol... more Solid-state batteries have many enticing advantages in terms of safety and stability, but the solid electrolytes upon which these batteries are based typically lead to high cell resistance. Both components of the resistance (interfacial, due to poor contact with electrolytes, and bulk, due to a thick electrolyte) are a result of the rudimentary manufacturing capabilities that exist for solid-state electrolytes. In general, solid electrolytes are studied as flat pellets with planar interfaces, which minimizes interfacial contact area. Here, multiple ink formulations are developed that enable 3D printing of unique solid electrolyte microstructures with varying properties. These inks are used to 3D-print a variety of patterns, which are then sintered to reveal thin, nonplanar, intricate architectures composed only of Li La Zr O solid electrolyte. Using these 3D-printing ink formulations to further study and optimize electrolyte structure could lead to solid-state batteries with dramati...
Scientific reports, Jan 13, 2018
To determine the safety of low-calcium-dialysate in patients undergoing maintenance hemodialysis ... more To determine the safety of low-calcium-dialysate in patients undergoing maintenance hemodialysis (MHD) and its effects on coronary artery calcification (CAC) and analyze clinical risk factors for CAC. A total of 174 MHD patients were recruited and randomly divided into two groups: high-calcium dialysate (HCD, 1.5 mmol/L Ca) and low-calcium dialysate (LCD, 1.25 mmol/L Ca). Changes in CAC score (CACS) and cardiac function were evaluated using spiral computed tomography and echocardiography, respectively. Clinical and laboratory parameters were measured. Intra-dialysis adverse reactions were recorded and compared between the two groups. CACS was significantly lower in the LCD group than in the HCD group by the end of the study. Cardiac E/A was significantly higher in the LCD group than in the HCD group by the end of the study. There was no significant difference in the frequency of any intra-dialysis adverse reactions between the two groups during the study. LCD is helpful in maintaini...
Water
Ecological engineering construction is the main method for urban riparian landscape restoration. ... more Ecological engineering construction is the main method for urban riparian landscape restoration. Continuous ecological environmental monitoring can reflect the effects of engineering construction and can provide a scientific basis for the improvement of engineering technology. However, the evaluation of riparian ecological engineering mainly focuses on the water environmental function and biodiversity conservation function after the construction period. Studies on the long-term evaluation of regional microclimate regulation and human settlement improvement are limited. In this paper, an ecological restoration project along the Yitong River in northern China was selected as the research object. Temperature, relative humidity and wind speed under different vegetation type combinations were monitored in the riparian ecological engineering construction during the first, second, third and tenth years. The temperature–humidity index (THI) was selected to evaluate human comfort and the imp...
Based on the survey of 154 farmers in Guiyang City, we analyze the basic situation of building of... more Based on the survey of 154 farmers in Guiyang City, we analyze the basic situation of building of grass-roots agricultural technology extension system in Guiyang City. Then we point out some problems in the building of grass-roots agricultural technology extension system in Guiyang City: the function of grass-roots agricultural technology extension center weakens; the content of agricultural technology extension is difficult to adapt to farmers' needs for technology; the extension mode of agricultural technology departments does not adapt to the needs of modern agriculture. In order to perfect the building of grass-roots agricultural technology extension system, the countermeasures and recommendations are put forth as follows: strengthening the input of funds, and ensuring that the basic work of public welfare agricultural technology extension is smoothly carried out; innovating upon the system, and improving the function of grass-roots agricultural technology extension center; ...
IEEE Transactions on Vehicular Technology, 2020
Advances in Building Technology, 2002
Publisher Summary This chapter proposes a new method of three-dimensional (3D) modeling based on ... more Publisher Summary This chapter proposes a new method of three-dimensional (3D) modeling based on the virtual reality (VR) and traditional CAD modeling technologies. World tool kit (WTK) is selected as the platform for VR environment. It is an advanced cross-platform development environment for high-performance, realtime 3D graphics applications. With the high-level application programmer's interface (API), end-users of WTK can write codes to call the functions in WTK function library, and then create the virtual world. To add and modify data of CAD models in a VR environment, a two-way data transformer between VR and CAD environment should be implemented. When the models are modified on AutoCAD, corresponding data in the VR environment should be automatically updated, and vice versa. Since the modeling functions of AutoCAD will be directly utilized, making changes to the structural models under AutoCAD will be convenient. In order to display the 3D models in a VR environment, data of models generated from AutoCAD must be translated into one of the formats that WTK can process. That is, after constructing the models under AutoCAD, users should save them in a DXF format file at first, and then use the WUM to open this DXF file, and make further conversion or map textures.
International Journal of Economics and Finance, 2020
Researchers have not come to an agreement on the impact of political connection on enterprise per... more Researchers have not come to an agreement on the impact of political connection on enterprise performance although this issue draws much attention. This paper attributes the above phenomenon to lack of precise classification of various political connection types in China. Based on existing literature, this paper divides political connections into current political connections and former political connections, and identifies their own hierarchy. Empirical study using Chinese Private Enterprise Survey data shows that different sorts of political connections all contribute to enterprise performance, and internal governance plays the mediating role. Overall, this paper may make marginal contributions to the study on the relationship between political connections and enterprise performance.
The purpose of this research is to address the problems of urban-rural dual economic structure, p... more The purpose of this research is to address the problems of urban-rural dual economic structure, promote the integr ated urban and rural development, and explore the difficulties of economic development in ethnic and border areas. Methods including principal components analysis are deployed to conduct comprehensive appraisal of regional ec onomic and social development, and in-depth analysis of development level, vitality, potential, characteristic, type and/or mode of towns in ethnic and border areas of Yunnan province. The development disparities, causes and prob lems in relation to towns in ethnic and border areas of Yunnan are summarized and analyzed. Policy implications a nd suggestions are proposed to develop small towns in the process of new rural construction and building a compre hensive well-off society in ethnic and border areas. Biography of Yang Wen As a senior statistician and associate professor, he got his bachelor in 1984 and master’s degree in 1991 from Yunnan Unive...
Leprosy Review, 2009
To evaluate the reliability and feasibility of two methods of multilocus variable number of tande... more To evaluate the reliability and feasibility of two methods of multilocus variable number of tandem repeat analysis (MLVA) for strain typing of M. leprae, and to study whether short tandem repeat loci are stable and suitable for epidemiological study of leprosy. Total DNA was extracted from skin biopsies of 20 new multibacillary (MB) patients from China diagnosed in 2006. To determine the copy numbers of short tandem repeats (STRs) for 13 loci, we amplified each locus individually by PCR, followed by sequence analysis of the amplicons. Separately, the same loci, plus four others were amplified by Multiplex PCRs (MP) using fluorescent primers and the copy number was identified by fragment length analysis (MP-FLA). MLVA was also performed at different times during treatment for a subset of the patients. Genetic variability of M. leprae in China can be assessed in microsatellite loci. (GTA)9 and (TTC)21 loci are hypervariable, with array sizes of 25 repeat units or more. The expansion of the (GTA)9 locus is a characteristic of some M. leprae isolates in China. A high level of allele concordance was observed between PCR-sequencing and MP-FLA methods. However, MP-FLA method was cost-effective, rapid, high throughput and suitable for strain typing. Five of the 20 isolates of M. leprae were from patients residing in the same township in Qiubei County, Yunnan, and matched closely by MLVA. Three of these patients are family contacts of previously diagnosed patients, with intra-familial strain types being similar, suggesting infections from common sources and transmission chain(s). The VNTR patterns were highly similar in biopsy and slit skin smears (SSS) before treatment, and in the SSS collected at various time points during treatment. Taken together, VNTR strain typing is a useful tool for study of short range transmission in leprosy.
2019 34th IEEE/ACM International Conference on Automated Software Engineering (ASE), 2019
We present PTracer, a Linux kernel patch trace bot based on an improved PatchNet. PTracer continu... more We present PTracer, a Linux kernel patch trace bot based on an improved PatchNet. PTracer continuously monitors new patches in the git repository of the mainline Linux kernel, filters out unconcerned ones, classifies the rest as bug-fixing or non bug-fixing patches, and reports bug-fixing patches to the kernel experts of commercial operating systems. We use the patches in February 2019 of the mainline Linux kernel to perform the test. As a result, PTracer recommended 151 patches to CGEL kernel experts out of 5,142, and 102 of which were accepted. PTracer has been successfully applied to a commercial operating system and has the advantages of improving software quality and saving labor cost.
2021 IEEE/ACM 13th International Workshop on Cooperative and Human Aspects of Software Engineering (CHASE), 2021
International Journal of Economics and Finance, 2020
Based on data of Chinese Private Enterprises Survey (CPES) from 2006-2014, this paper uses OLS mo... more Based on data of Chinese Private Enterprises Survey (CPES) from 2006-2014, this paper uses OLS model and other empirical methods to estimate the impacts of political connections on private enterprise performance in China, as well as heterogeneous effects and mediating effects of different types of political connections on tax burden and non-productive activities expense. The results show that, political connections contribute significantly to private enterprises. Compared with previous political connections, like working experiences in state-owned enterprises and government-affiliated institutions, current political connections, like deputies to the NPC or members of CPPCC, have played better and further roles in enterprise performance. Tax burden and non-productive activities expense have a mediation effect in the relationship between political connections and enterprise performance. This study replenishes new evidence to describe how political connections affect private enterprise...
Water, 2020
Small- and medium-sized rivers are facing a serious degradation of ecological function in water r... more Small- and medium-sized rivers are facing a serious degradation of ecological function in water resource-scarce regions of Northern China. Reservoir ecological operation can restore the damaged river ecological environment. Research on reservoir ecological operation and watershed management of urban rivers is limited in cold regions of middle and high latitudes. In this paper, the urban section of the Yitong River was selected as the research object in Changchun, Northern China. The total ecological water demand and reservoir operation water (79.35 × 106 m3 and 15.52 × 106 m3, respectively) were calculated by the ecological water demand method, and a reservoir operation scheme was established to restore the ecological function of the urban section of the river. To examine the scientific basis and rationality of the operation scheme, the water quality of the river and physical habitat after carrying out the scheme were simulated by the MIKE 11 one-dimensional hydrodynamic-water quali...
ECS Meeting Abstracts, 2014
Metabolites, 2019
Copper (Cu) is widely used in the swine industry to improve the growth performance of pigs. Howev... more Copper (Cu) is widely used in the swine industry to improve the growth performance of pigs. However, high doses of copper will induce cell damage and toxicity. The aim of this study was to evaluate toxicity, bioavailability, and effects on metabolic processes of varying copper sources using porcine intestinal epithelial cells (IPEC-J2) as a model. The IPEC-J2 were treated with two doses (30 and 120 μM) of CuSO4, Cu Glycine (Cu-Gly), and Cu proteinate (Cu-Pro) for 10 h, respectively. Cell damage and cellular copper metabolism were measured by the changes in cell viability, copper uptake, oxidative stress biomarkers, and gene/protein expression levels. The results showed that cell viability and ratio of reduced and oxidized glutathione (GSH/GSSG) decreased significantly in all treatment groups; intracellular copper content increased significantly in all treatment groups; total superoxide dismutase (SOD) activity increased significantly in the 120 μM exposed groups; SOD1 protein expres...
International Journal of Agricultural and Biological Engineering, 2017
Fast pyrolysis of corn stover was performed at temperatures in the range of 450°C-525°C in a flui... more Fast pyrolysis of corn stover was performed at temperatures in the range of 450°C-525°C in a fluidized bed. The chemical composition of the bio-oil acquired was analyzed by GC/MS, and its pH, kinetic viscosity and calorific values were determined. In the pyrolysis system used in this experiment, some improvements to former pyrolysis systems were done. Two screw feeders were used to prevent jamming the feeding system, and the condenser was equipped with some nozzles and a heat exchanger to cool quickly the cleaned hot gas into bio-oil. The results showed that the bio-oil yield increased with increasing pyrolysis temperature and then declined with a further increase in pyrolysis temperature. The highest bio-oil yield of 43.3 wt.% was obtained at 500°C with the dolomite as bed material. The char yield always decreased with the increase of temperature. The major chemical compounds of bio-oil included acetol, butanone, acetic acid, propionic acid, ethylene glycol, phenol, etc. Pyrolysis oil was completely immiscible in diesel, but homogeneous emulsions were obtained when mixing the pyrolysis oil, emulsifier with diesel in different ratios.
Dental materials journal, Jan 14, 2018
The objective of this study was to investigate the effects of a silicate-based composite material... more The objective of this study was to investigate the effects of a silicate-based composite material on proliferation and mineralization of human dental pulp cells (hDPCs), which was compared with those of calcium hydroxide (Ca(OH), CH) and tricalcium silicate (CaSiO, C3S). HDPCs were cultured with CH, C3S and tricalcium silicate/dicalcium silicate (CaSiO/CaSiO, C3S/C2S) composites extract. The CCK-8 assay showed that the composite material stimulated the proliferation of hDPCs. The odontogenic marker genes and DSPP protein expression were more significantly up-regulated by the C3S/C2S composite material compared with pure CH and C3S. HDPCs cultured with composite material extract exert stronger ALP activity and alizarin red S staining. C3S/C2S composite material was advantageous over pure C3S by showing enhanced ability to stimulate the proliferation and odontogenic differentiation of hDPCs, suggesting that the C3S/C2S composite materials possess desirable biocompatibility and bioacti...
Water science and technology : a journal of the International Association on Water Pollution Research, 2018
Anaerobic digestion with thermal hydrolysis pretreatment (THP), also called advanced anaerobic di... more Anaerobic digestion with thermal hydrolysis pretreatment (THP), also called advanced anaerobic digestion (AAD), is a mainstream technology for sludge treatment. AAD changes sludge, it can degrade extracellular polymeric substances (EPS), release EPS from the sludge, and alter the particle size distribution. We synthesized PFS-PDMDAAC from the inorganic coagulant polyferric sulfate (PFS) and the organic coagulant polymer polydimethyldiallylammonium chloride (PDMDAAC) in various PFS:PDMDAAC weight ratios. We investigated the effects of PFS-PDMDAAC pretreatment on AAD sludge dewaterability, and developed an explanation for them. Capillary suction time (CST) was used as a measure of sludge dewaterability. Dissolved organic matter, the three-dimensional excitation emission matrix, particle size (d), zeta potential, and sludge microstructure were observed in order to explain changes in sludge dewaterability that resulted from different compositions and dosages of coagulants. Treatment wit...
Shanghai kou qiang yi xue = Shanghai journal of stomatology, 2017
To construct an expression vector of a small hairpin RNA (shRNA) targeting human PERK gene and to... more To construct an expression vector of a small hairpin RNA (shRNA) targeting human PERK gene and to observe gene-silencing effects of PERK in human dental pulp cells (DPCs). According to PERK gene cDNA sequence, shRNA was designed and synthesized, which was then annealed into hU6-MCS-CMV-EGFP vector. After identified by sequencing, hU6-MCS-CMV-EGFP vector and packaging vector were co-transfected into 293 T cells. 72 hours later, the recombinant lentiviruses were obtained after harvesting and concentrating. Then LV-PERK-RNAi vectors were transfected into DPCs at an appropriate multiplicity of infection. To verify the interference effect, real- time PCR and Western blot were used to detect expression levels of PERK mRNA and protein in the transfected DPCs. The data were analyzed with SPSS 24.0 software package. LV-PERK-RNAi vectors were successfully constructed with a high titer of 3×10 TU/mL. The results of RT-PCR and Western blot demonstrated that after infection with LV-PERK-RNAi vec...
Experimental and therapeutic medicine, 2018
The objective of the present study was to examine whether supplementation has a role in the regul... more The objective of the present study was to examine whether supplementation has a role in the regulation of the intestinal flora and the development of the immune system of neonatal mice. A total of 30 pregnant BALB/c mice, including their offspring, were randomly divided into three groups: In the maternal intervention group (Ba), maternal mice were treated with from birth until weaning at postnatal day 21 (PD21) followed by administration of saline to the offspring at PD21-28; in the offspring intervention group (Ab), breast-feeding maternal mice were supplemented with saline and offspring were directly supplemented with from PD21-28; in the both maternal and offspring intervention group (Bb), both maternal mice and offspring were supplemented with at PD 0-21 and at PD21-28. While mice in the control group were given the same volume of normal saline. Stool samples from the offspring were collected at PD14, -21 and -28 to observe the intestinal flora by colony counts of spp., spp., sp...
Advanced materials (Deerfield Beach, Fla.), 2018
Solid-state batteries have many enticing advantages in terms of safety and stability, but the sol... more Solid-state batteries have many enticing advantages in terms of safety and stability, but the solid electrolytes upon which these batteries are based typically lead to high cell resistance. Both components of the resistance (interfacial, due to poor contact with electrolytes, and bulk, due to a thick electrolyte) are a result of the rudimentary manufacturing capabilities that exist for solid-state electrolytes. In general, solid electrolytes are studied as flat pellets with planar interfaces, which minimizes interfacial contact area. Here, multiple ink formulations are developed that enable 3D printing of unique solid electrolyte microstructures with varying properties. These inks are used to 3D-print a variety of patterns, which are then sintered to reveal thin, nonplanar, intricate architectures composed only of Li La Zr O solid electrolyte. Using these 3D-printing ink formulations to further study and optimize electrolyte structure could lead to solid-state batteries with dramati...
Scientific reports, Jan 13, 2018
To determine the safety of low-calcium-dialysate in patients undergoing maintenance hemodialysis ... more To determine the safety of low-calcium-dialysate in patients undergoing maintenance hemodialysis (MHD) and its effects on coronary artery calcification (CAC) and analyze clinical risk factors for CAC. A total of 174 MHD patients were recruited and randomly divided into two groups: high-calcium dialysate (HCD, 1.5 mmol/L Ca) and low-calcium dialysate (LCD, 1.25 mmol/L Ca). Changes in CAC score (CACS) and cardiac function were evaluated using spiral computed tomography and echocardiography, respectively. Clinical and laboratory parameters were measured. Intra-dialysis adverse reactions were recorded and compared between the two groups. CACS was significantly lower in the LCD group than in the HCD group by the end of the study. Cardiac E/A was significantly higher in the LCD group than in the HCD group by the end of the study. There was no significant difference in the frequency of any intra-dialysis adverse reactions between the two groups during the study. LCD is helpful in maintaini...