zahra kayani - Academia.edu (original) (raw)

Papers by zahra kayani

Journal of biomedical physics and engineering, 2024

Background: Photothermal therapy (PTT) is one of the effective and non-invasive strategies which ... more Background: Photothermal therapy (PTT) is one of the effective and non-invasive strategies which hold great promise for improving the treatment of cancer cells. PTT is based on activating a photosensitizer by infrared light irradiation and producing heat and reactive species and apoptosis in the tumor area. Objective: The aim of this study was to investigate the effect of photothermal/ chemotherapy on melanoma cancer cells using poly(2-amino phenol)/gold (P2AO/ AuNPs) and doxorubicin (DOX). Material and Methods: In this experimental study, nanoparticles of P2AO/ AuNPs were synthesized, and their mixture with DOX was applied as a photosensitizer for photothermal/chemotherapy of a C540 (B16-F10) melanoma cell line. Results: P2AO/AuNPs generated heat and cytotoxic responsive oxygen species (ROS) upon 808-nm light irradiation with simultaneous intensifying DOX therapeutic effect under domination of synergism effects between light irradiation, P2AO/AuNPs, and doxorubicin. Cell treatment with both P2AO/AuNPs and DOX resulted in a considerable increase in necroptotic cells to 61% with a significant decrease in the living cells (39%). Conclusion: P2AO/AuNPs provided a platform for light absorption and intensifying DOX therapeutic effect. This study approved the applicability of a new photothermal/chemotherapy by domination of synergistic effects attained by combination of laser light, P2AO, AuNPs, and DOX.

Ultrasound in medicine & biology, Mar 1, 2024

Journal of pharmaceutical sciences, May 1, 2024

Scientific Reports, Feb 11, 2021

Aberrant activation of c-Met signalling plays a prominent role in cancer development and progress... more Aberrant activation of c-Met signalling plays a prominent role in cancer development and progression. A series of 12 imidazo [1,2-α] pyridine derivatives bearing 1,2,3-triazole moiety were designed, synthesized and evaluated for c-Met inhibitory potential and anticancer effect. The inhibitory activity of all synthesized compounds against c-Met kinase was evaluated by a homogeneous timeresolved fluorescence (HTRF) assay at the concentration range of 5-25 µM. Derivatives 6d, 6e and 6f bearing methyl, tertiary butyl and dichloro-phenyl moieties on the triazole ring, respectively, were the compounds with the highest potential. They significantly inhibited c-Met by 55.3, 53.0 and 51.3%, respectively, at the concentration of 25 µM. Synthetic compounds showed antiproliferative effects against lung (EBC-1) and pancreatic cancer cells (AsPc-1, Suit-2 and Mia-PaCa-2) expressing different levels of c-Met, with IC 50 values as low as 3.0 µM measured by sulforhodamine B assay. Active derivatives significantly blocked c-Met phosphorylation, inhibited cell growth in three-dimensional spheroid cultures and also induced apoptosis as revealed by Annexin V/propidium iodide flow cytometric assay in AsPc-1 cells. They also inhibited PDGFRA and FLT3 at 25 µM among a panel of 16 kinases. Molecular docking and dynamics simulation studies corroborated the experimental findings and revealed possible binding modes of the select derivatives with target receptor tyrosine kinases. The results of this study show that some imidazopyridine derivatives bearing 1,2,3-triazole moiety could be promising molecularly targeted anticancer agents against lung and pancreatic cancers. Cancer continues to be a major health burden being the first or second common cause of death before the age of 70 in 91 countries and accounting for 9.6 million deaths in 2018 worldwide 1. Several recent studies have focused on finding new therapies that target specific signalling pathways in cancer cells and in particular on small molecules targeting aberrant kinases 2. Receptor tyrosine kinases (RTKs) play critical roles in cell proliferation, survival, migration, invasion and other hallmarks of cancer. Aberrant RTKs activation is associated with disease progression in a variety of human malignancies, making them promising drug targets for cancer treatment 3. Hepatocyte growth factor receptor or mesenchymal-epithelial transition factor (c-MET) is an important RTK essential for several cellular processes 4,5. Aberrant activation of hepatocyte growth factor (HGF)/c-Met pathway due to MET gene overexpression, amplification, activating mutations, or excessive autocrine or paracrine HGF secretion have been associated with the development of several cancers such as lung, pancreas, gastric, breast, kidney, bladder, ovary, brain and prostate cancers 6-9 .

Biomedicine & Pharmacotherapy, Nov 1, 2018

Chemotherapy constitutes the main strategy in management of breast cancer (BC). Lack of specifici... more Chemotherapy constitutes the main strategy in management of breast cancer (BC). Lack of specificity and high burden of adverse effects of chemotherapeutic agents remain the most important impediments to successful treatment of BC patients. Folate receptor α (FRα) could be very promising for therapeutic targeting in this type of cancer. In this study, ß-lactoglobulin nanoparticles (BNPs) conjugated with folic acid and loaded with doxorubicin (FDBNPs) were prepared. Various characterization techniques were applied to determine the size, polydispersity and doxorubicin loading of prepared FDBNPs in comparison with doxorubicin-loaded BNPs (DBNPs). The results showed that FDBNPs are 109.77 ± 2.80 nm in diameter with well dispersed and spherical shapes. The biodegradation of FDBNPs in the presence of trypsin enzyme and in PBS at different pH (4 and 7) was spectrophotometrically monitored and the results showed that the FDBNPs with encapsulation efficiency of 68.82% ± 1.76% could deliver doxorubicin at clinically relevant doses. Effects of DBNPs and FDBNPs against MCF-7 and MDA-MB-231, BC and triple negative BC (TNBC) cell lines, respectively, showed significant inhibition of cell proliferation as well as induction of apoptosis. Based on these findings, FDBNPs with facilitated drug release and targeted doxorubicin delivery capacities could have high therapeutic potential for BC and TNBC.

International Journal of Biological Macromolecules, Feb 1, 2018

Highlights  Doughnut-shaped BSA nanoparticles were synthesized for the first time  The higher l... more Highlights  Doughnut-shaped BSA nanoparticles were synthesized for the first time  The higher loading capacity of doughnut-shaped BSA nanoparticles for doxorubicin in versus spherical-shaped  Killing cancer cells and overcoming multidrug resistance cancer cells by using doughnutshaped BSA nanoparticles

Photodiagnosis and Photodynamic Therapy, Mar 1, 2021

Ultrasound in Medicine & Biology

Iranian Journal of Pharmaceutical Research : IJPR, 2021

Cancer is the second cause of death in the world and the discovery of novel anticancer agents is ... more Cancer is the second cause of death in the world and the discovery of novel anticancer agents is of vital importance to provide better therapeutic options for cancer patients. In this study, a new series of 12 arylidene hydrazone phenanthrotriazine derivatives were designed, synthesized, and tested in-vitro for antiproliferative activity against three cancer cell lines including colorectal cancer (HT-29), breast cancer (MCF-7) and leukemia (MOLT-4) cells and also against Vero normal cells. The effect of derivatives on cell cycle and apoptosis induction were studied by flow cytometric propidium iodide/RNase assay and Hoechst 33258 staining, respectively, while docking analysis was used to investigate the interactions of synthesized derivatives with the c-Met receptor kinase domain. Some compounds showed considerable antiproliferative activity against tested cancer cells. The most potent derivative was 9k bearing pyrrole moiety with IC50 values of 14.3, 4.7 and 1.7 µM against HT-29, M...

Journal of Drug Delivery Science and Technology, 2021

Abstract Conventional cancer therapy often fails for an insufficient therapeutic outcome, tumor h... more Abstract Conventional cancer therapy often fails for an insufficient therapeutic outcome, tumor heterogeneity, and drug resistance. The past decade has witnessed that combination therapies using near-infrared (NIR) light-mediated photothermal therapy (PTT) with its great capacity of heat ablation in tumor tissue enhanced the efficacy of radiotherapy (RT) and reduced radiotoxic effects on normal tissue. Metallic-nanoplatforms, such as transition metal dichalcogenides, gold, copper and, platinum nanoparticles have attracted lots of attention in nano biomedicine. Because of their desired properties such as tunable surface plasmon resonance (SPR), high plasmonic photothermal activity, and high-Z number with stronger photoelectric effects could serve as photosensitizers, and radiosensitizers to improve the efficacy of PTT and RT. Dual radiation of laser light and X-ray into metallic-nanoplatforms considerably improved the cancer treatment via reactive oxygen species (ROS) production. Metallic-nanoplatforms can act as a dual absorber of laser light and X-ray, a common sensitizer, for treatment of cancer. Therefore, in this review, we tried to summarize the developing metallic-nanoplatforms in PTT, RT, and combination therapy.

Photodiagnosis and Photodynamic Therapy, 2021

Journal of agricultural and food chemistry, Jan 8, 2017

Cancer is the major cause of morbidity and mortality worldwide. Hydroxycinnamic acids (HCAs) are ... more Cancer is the major cause of morbidity and mortality worldwide. Hydroxycinnamic acids (HCAs) are naturally occurring compounds and their alkyl esters may possess enhanced biological activities. We evaluated C4, C14, C16, and C18 alkyl esters of p-coumaric, ferulic, sinapic, and caffeic acids (19 compounds) for their cytotoxic activity against four human cancer cells and also examined their effect on cell cycle alteration and apoptosis induction. The tetradecyl (1c) and hexadecyl (1d) esters of p-coumaric acid and tetradecyl ester of caffeic acid (4c), but not the parental HCAs, were selectively effective against MOLT-4 (human lymphoblastic leukemia) cells with IC50 values of 0.123 ± 0.012, 0.301 ± 0.069 and 1.0 ± 0.1 μM, respectively. Compounds 1c, 1d, and 4c significantly increased apoptotic cells in sub-G1 phase and activated the caspase-3 enzyme in MOLT-4 cells. Compound 1c was 15.4 and 23.6 times more potent than doxorubicin and cisplatin, respectively, against the drug resistan...

Journal of the Iranian Chemical Society, 2015

International journal of biological macromolecules, Jan 10, 2017

Traditional spherical albumin nanoparticles remain as the dominant shape of nano-carriers describ... more Traditional spherical albumin nanoparticles remain as the dominant shape of nano-carriers described in the literature at present, due to their simple desolvation method of synthesis. However, non-spherical shapes also show great promise as cancer drug delivery vectors. In this study, we report a novel synthetic strategy based on dimethyl sulfoxide (DMSO) addition during desolvation step, to produce doughnut-shaped bovine serum albumin nanoparticles (DBSA-NPs), while maintaining narrow size distributions and homogeneity. The characteristics such as size, polydispersity and doxorubicin loading of prepared DBSA-NPs in comparison with spherical ones were determined. The biodegradation of DBSA-NPs loaded with doxorubicin (Dox-DBSA-NPs) in the presence of trypsin enzyme was spectrophotometrically monitored directly based on doxorubicin release profile. The release profile was analyzed with different kinetic models and it was best fitted with Higuchi kinetics model. The anticancer effect o...

Scientific Reports, 2021

Biomedicine & Pharmacotherapy, 2018