zinat entezami - Academia.edu (original) (raw)

Papers by zinat entezami

Research paper thumbnail of Determination of lymphocytes surface markers in patients with thermal burns and the influence of burn size on mononuclear cell subsets

Medical Journal of the Islamic Republic of Iran, 2017

The immunology of burn injuries is an important cause of public health problems worldwide. In In ... more The immunology of burn injuries is an important cause of public health problems worldwide. In In Iran, burn injuries have yielded significant morbidity and mortality.

Research paper thumbnail of Enumeration of Peripheral Blood Lymphocyte Subsets in a Healthy Iranian Population

Objective-This study was conducted to determine the reference intervals for CD3 + (T cells), CD4 ... more Objective-This study was conducted to determine the reference intervals for CD3 + (T cells), CD4 + (T helper/inducer cell, Th), CD8 + (T suppressor/cytotoxic cells, Ts/c), CD3 + CD4 + / CD3 + CD8 + ratio, CD19 + (B cells) and CD16 + +56 + (NK cells) in a healthy Iranian population. Method-Becton-Dickinson Immunocytometry Systems (BDIS) Simultest IMK-lymphocyte (dual color) reagents were used. The samples were first stained with appropriate monoclonal antibodies and then lysed using BDIS FACS lysing solution. The samples were analysed by BDIS FACS Caliber flowcytometry using the SimulSET software, version 2.5. Results-The study population consisted of 152 healthy, male and female non-smokers, 18 to 60 years of age. The mean CD3 + percentage was 69.95% for males and 71.46% for females, and the mean percentage of CD3 + CD4 + Th subset was 40.4% and 44.21% for males and females respectively. The mean CD3 + CD4 + /CD3 + CD8 + ratio was 1.55±0.54 for male population and 1.7±0.65 for female populations. The mean percentage of B lymphocytes (CD19 +) was 14.52% for the females and 13.08% for males. Conclusion-This study indicates that race, sex and age should be a consideration in the evaluation of the immune status.

Research paper thumbnail of Increased activation and expansion of a CD57+ subset within peripheral CD8+ T lymphocytes in Mycobacterium tuberculosis-infected patients

Archives of Iranian medicine, 2006

Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of e... more Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of extracellular pathogens by limiting M.tuberculosis replication. Alterations in cytolytic function, inappropriate maturation/differentiation, and limited proliferation could reduce their ability to control M.tuberculosis replication. In an attempt to further characterize the immune responses during M.tuberculosis infection, we enumerated gamma-delta and alpha-beta receptor-bearing T cells expressing CD8 or CD4 phenotype and analyzed the differentiation phenotypes of CD8+ and CD4+ T lymphocyte subpopulations in 47 cases (23 new cases and 24 multidrug resistant patients) and 20 control subjects, using flowcytometry. We found that the CD4/CD8 ratio was significantly lower in newly-diagnosed M.tuberculosis patients compared to multidrug resistant and control subjects (P < 0.003). Also, we found that a large proportion of CD8+ T lymphocytes in newly-diagnosed patients was defined by increase...

Research paper thumbnail of Enumeration of Peripheral Blood Lymphocyte Subsets in a Healthy Iranian Population

Objective-This study was conducted to determine the reference intervals for CD3+ (T cells), CD4+ ... more Objective-This study was conducted to determine the reference intervals for CD3+ (T cells), CD4+ (T helper/inducer cell, Th), CD8+ (T suppressor/cytotoxic cells, Ts/c), CD3+CD4+/ CD3+CD8+ ratio, CD19+ (B cells) and CD16++56+ (NK cells) in a healthy Iranian population. Method-Becton-Dickinson Immunocytometry Systems (BDIS) Simultest IMK-lymphocyte (dual color) reagents were used. The samples were first stained with appropriate monoclonal antibodies and then lysed using BDIS FACS lysing solution. The samples were analysed by BDIS FACS Caliber flowcytometry using the SimulSET software, version 2.5. Results- The study population consisted of 152 healthy, male and female non-smokers, 18 to 60 years of age. The mean CD3+ percentage was 69.95 % for males and 71.46 % for females, and the mean percentage of CD3+ CD4+ Th subset was 40.4 % and 44.21 % for males and females respectively. The mean CD3+ CD4+/CD3+ CD8+ ratio was 1.55±0.54 for male population and 1.7±0.65 for female populations. Th...

Research paper thumbnail of Increased activation and expansion of a CD57+ subset within peripheral CD8+ T lymphocytes in Mycobacterium tuberculosis-infected patients

Archives of Iranian medicine, 2006

BACKGROUND Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the ... more BACKGROUND Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of extracellular pathogens by limiting M.tuberculosis replication. Alterations in cytolytic function, inappropriate maturation/differentiation, and limited proliferation could reduce their ability to control M.tuberculosis replication. METHODS In an attempt to further characterize the immune responses during M.tuberculosis infection, we enumerated gamma-delta and alpha-beta receptor-bearing T cells expressing CD8 or CD4 phenotype and analyzed the differentiation phenotypes of CD8+ and CD4+ T lymphocyte subpopulations in 47 cases (23 new cases and 24 multidrug resistant patients) and 20 control subjects, using flowcytometry. RESULTS We found that the CD4/CD8 ratio was significantly lower in newly-diagnosed M.tuberculosis patients compared to multidrug resistant and control subjects (P < 0.003). Also, we found that a large proportion of CD8+ T lymphocytes in newly-diagnosed patie...

Research paper thumbnail of The immunomodulatory effects of low molecular weight garlic protein in crosstalk between peripheral blood mononuclear cells and colon cancer cells

Process Biochemistry, 2021

Garlic is an ancient plant whose medicinal usage has a long traditional history. It has been alwa... more Garlic is an ancient plant whose medicinal usage has a long traditional history. It has been always advocated as a suitable candidate for immunomodulation. The purpose of the present study was to assess the immunomodulatory effects of Low Molecular Weight Garlic Proteins (LMWGP) on the proliferation of peripheral blood mononuclear cells (PBMCs), induction of regulatory T cells (Tregs), as well as cytokine secretion in the co-culture of PBMCs and colon cancer cell lines, SW48. Garlic cloves were initially extracted and then protein fractions were separated using gel filtration chromatography and analyzed with mass spectrometry (MS). The effects of LMWGP on the proliferation of PBMCs and induction of Tregs were later analyzed by flow cytometry. Cytokine secretion was also evaluated using the ELISA test. The findings revealed that the proliferation of PBMCs had significantly augmented in the presence of LMWGP (p ≤ 0.05). Moreover, this fraction had reduced induction of Tregs in the co-...

Research paper thumbnail of Increased Activation and Expansion of a CD57+ Subset Within Peripheral CD8+ T Lymphocytes in Mycobacterium Tuberculosis-Infected Patients

Archives of Iranian Medicine, Feb 1, 2006

Background: Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the... more Background: Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of extracellular pathogens by limiting M.tuberculosis replication. Alterations in cytolytic function, inappropriate maturation/differentiation, and limited proliferation could reduce their ability to control M.tuberculosis replication. Methods: In an attempt to further characterize the immune responses during M.tuberculosis infection, we enumerated γδ and αβ receptor-bearing T cells expressing CD8 or CD4 phenotype and analyzed the differentiation phenotypes of CD8+ and CD4+ T lymphocyte subpopulations in 47 cases (23 new cases and 24 multidrug resistant patients) and 20 control subjects, using flowcytometry. Results: We found that the CD4/CD8 ratio was significantly lower in newly-diagnosed M.tuberculosis patients compared to multidrug resistant and control subjects (P < 0.003). Also, we found that a large proportion of CD8+ T lymphocytes in newly-diagnosed patients was defined by increased surface expression of CD57 as compared to the two other settings (P < 0.002). This increase was more profound in patients with an inverted CD4/CD8 ratio. Analysis of the late activation antigen revealed that this was predominantly HLA-DR+ (P < 0.003). No significant changes were observed in the percentages of CD8+CD57+ T cells between the different settings. Moreover, the co-stimulatory molecule CD28+ tended to be underexpressed by CD8+ T cells in multidrug resistant patients when compared to newly-diagnosed subjects (P < 0.002), but not to the control subjects. In contrast, the frequency of CD28+ marker on CD4+ T cells was higher in the setting of multidrug resistant compared with those of new cases (P < 0.0001). No significant changes were observed in percentages of γδ receptor-bearing T cells between different groups. Conclusion: We suggest that the increase in the proportion of CD57+ within CD8+ T cells in newly-diagnosed patients results from M.tuberculosis antigenic stimulation, which is a hallmark of many infections and that the protracted accumulation of CD57+ T lymphocytes might reflect an end-stage differentiation phenotype.

Research paper thumbnail of Increased activation and expansion of a CD57+ subset within peripheral CD8+ T lymphocytes in Mycobacterium tuberculosis-infected patients

Archives of Iranian medicine, 2006

Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of e... more Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of extracellular pathogens by limiting M.tuberculosis replication. Alterations in cytolytic function, inappropriate maturation/differentiation, and limited proliferation could reduce their ability to control M.tuberculosis replication. In an attempt to further characterize the immune responses during M.tuberculosis infection, we enumerated gamma-delta and alpha-beta receptor-bearing T cells expressing CD8 or CD4 phenotype and analyzed the differentiation phenotypes of CD8+ and CD4+ T lymphocyte subpopulations in 47 cases (23 new cases and 24 multidrug resistant patients) and 20 control subjects, using flowcytometry. We found that the CD4/CD8 ratio was significantly lower in newly-diagnosed M.tuberculosis patients compared to multidrug resistant and control subjects (P < 0.003). Also, we found that a large proportion of CD8+ T lymphocytes in newly-diagnosed patients was defined by increase...

Research paper thumbnail of Local eosinophils are associated with increased IgA subclass levels in the sinonasal mucosa of chronic rhinosinusitis with polyp patients

Allergy, Asthma & Clinical Immunology, Apr 25, 2020

Background: Chronic rhinosinusitis (CRS) describes an inflammatory condition affecting the sinona... more Background: Chronic rhinosinusitis (CRS) describes an inflammatory condition affecting the sinonasal mucosa. As the immune system players such as immunoglobulins play prominent roles in the development of CRS, we aimed to investigate the expression of IgA subclasses and factors involved in IgA class switching in the sinonasal mucosa of CRS patients. Methods: Specimens were collected from the sinonasal mucosa of the healthy controls and CRS patients. Histological assessments were performed by H&E and immunohistochemistry. Real-time PCR and ELISA methods were applied to measure gene expression and protein levels extracted from tissue samples, respectively. Results: We observed that total IgA and subclass-positive cells were higher in the patient groups than controls. There was a significant correlation between the number of eosinophils and total IgA and subclasses-positive cells (Pv < 0.0001). The expression of CXCL13, BAFF, AID, and germline transcripts were increased in CRSwNP patients. In contrast to IgA2 levels, IgA1 levels were significantly increased in the sinonasal tissue of CRSwNP patients (Pv < 0.01). TGF-β was significantly elevated in the sinonasal tissue of patients with CRSsNP. Conclusions: Increased protein levels of IgA subclasses and related antibody-producing cells were associated with elevated eosinophils in CRSwNP patients which may result in eosinophil pathological functions. Several therapeutic approaches might be developed to modulate the IgA production to ameliorate the inflammatory mechanisms in CRSwNP patients.

Research paper thumbnail of Levels of total IgA and IgA subclasses in the serum of chronic rhinosinusitis patients

Medical Journal of the Islamic Republic of Iran

Although IgE levels increase in the sera of patients with nasal polyps, and most studies have exa... more Although IgE levels increase in the sera of patients with nasal polyps, and most studies have examined its rate and role in these patients, IgA has not been studied as the most abundant antibody after IgG in sera of patients with CRS. →What this article adds: There is no significant difference in total IgA, IgA1, and IgA2 levels in serum of patients with CRS in comparison to healthy controls. Therefore, serum IgA is not a good marker for diagnosis and prognosis of CRS, consequently local investigation of these biomarkers may result in better interpretation.

Research paper thumbnail of Enumeration of Peripheral Blood Lymphocyte Subsets in a Healthy Iranian Population

Objective-This study was conducted to determine the reference intervals for CD3 + (T cells), CD4 ... more Objective-This study was conducted to determine the reference intervals for CD3 + (T cells), CD4 + (T helper/inducer cell, Th), CD8 + (T suppressor/cytotoxic cells, Ts/c), CD3 + CD4 + / CD3 + CD8 + ratio, CD19 + (B cells) and CD16 + +56 + (NK cells) in a healthy Iranian population. Method-Becton-Dickinson Immunocytometry Systems (BDIS) Simultest IMK-lymphocyte (dual color) reagents were used. The samples were first stained with appropriate monoclonal antibodies and then lysed using BDIS FACS lysing solution. The samples were analysed by BDIS FACS Caliber flowcytometry using the SimulSET software, version 2.5. Results-The study population consisted of 152 healthy, male and female non-smokers, 18 to 60 years of age. The mean CD3 + percentage was 69.95% for males and 71.46% for females, and the mean percentage of CD3 + CD4 + Th subset was 40.4% and 44.21% for males and females respectively. The mean CD3 + CD4 + /CD3 + CD8 + ratio was 1.55±0.54 for male population and 1.7±0.65 for female populations. The mean percentage of B lymphocytes (CD19 +) was 14.52% for the females and 13.08% for males. Conclusion-This study indicates that race, sex and age should be a consideration in the evaluation of the immune status.

Research paper thumbnail of T-Lymphocyte Subsets in Bronchoalveolar Lavage: A Flow-Cytometric Analysis and Its Importance in the Differential Diagnosis of Sarcoidosis

Background - The immunophenotyping of lymphocyte subsets in bronchoalveolar lavage (BAL) is of im... more Background - The immunophenotyping of lymphocyte subsets in bronchoalveolar lavage (BAL) is of importance in the differential diagnosis of interstitial lung diseases. The main intent for lymphocyte population subtyping in BAL fluid is finding those diseases with increased lymphocytic cell infiltration in the affected organ such as sarcoidosis and asbestosis. Methods - A dual color flow-cytometric analysis was performed to

Research paper thumbnail of 131 The role of T-cell subsets in burns patients

International Journal of Infectious Diseases, 2006

Research paper thumbnail of Determination of lymphocytes surface markers in patients with thermal burns and the influence of burn size on mononuclear cell subsets

Medical Journal of the Islamic Republic of Iran, 2017

The immunology of burn injuries is an important cause of public health problems worldwide. In In ... more The immunology of burn injuries is an important cause of public health problems worldwide. In In Iran, burn injuries have yielded significant morbidity and mortality.

Research paper thumbnail of Enumeration of Peripheral Blood Lymphocyte Subsets in a Healthy Iranian Population

Objective-This study was conducted to determine the reference intervals for CD3 + (T cells), CD4 ... more Objective-This study was conducted to determine the reference intervals for CD3 + (T cells), CD4 + (T helper/inducer cell, Th), CD8 + (T suppressor/cytotoxic cells, Ts/c), CD3 + CD4 + / CD3 + CD8 + ratio, CD19 + (B cells) and CD16 + +56 + (NK cells) in a healthy Iranian population. Method-Becton-Dickinson Immunocytometry Systems (BDIS) Simultest IMK-lymphocyte (dual color) reagents were used. The samples were first stained with appropriate monoclonal antibodies and then lysed using BDIS FACS lysing solution. The samples were analysed by BDIS FACS Caliber flowcytometry using the SimulSET software, version 2.5. Results-The study population consisted of 152 healthy, male and female non-smokers, 18 to 60 years of age. The mean CD3 + percentage was 69.95% for males and 71.46% for females, and the mean percentage of CD3 + CD4 + Th subset was 40.4% and 44.21% for males and females respectively. The mean CD3 + CD4 + /CD3 + CD8 + ratio was 1.55±0.54 for male population and 1.7±0.65 for female populations. The mean percentage of B lymphocytes (CD19 +) was 14.52% for the females and 13.08% for males. Conclusion-This study indicates that race, sex and age should be a consideration in the evaluation of the immune status.

Research paper thumbnail of Increased activation and expansion of a CD57+ subset within peripheral CD8+ T lymphocytes in Mycobacterium tuberculosis-infected patients

Archives of Iranian medicine, 2006

Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of e... more Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of extracellular pathogens by limiting M.tuberculosis replication. Alterations in cytolytic function, inappropriate maturation/differentiation, and limited proliferation could reduce their ability to control M.tuberculosis replication. In an attempt to further characterize the immune responses during M.tuberculosis infection, we enumerated gamma-delta and alpha-beta receptor-bearing T cells expressing CD8 or CD4 phenotype and analyzed the differentiation phenotypes of CD8+ and CD4+ T lymphocyte subpopulations in 47 cases (23 new cases and 24 multidrug resistant patients) and 20 control subjects, using flowcytometry. We found that the CD4/CD8 ratio was significantly lower in newly-diagnosed M.tuberculosis patients compared to multidrug resistant and control subjects (P < 0.003). Also, we found that a large proportion of CD8+ T lymphocytes in newly-diagnosed patients was defined by increase...

Research paper thumbnail of Enumeration of Peripheral Blood Lymphocyte Subsets in a Healthy Iranian Population

Objective-This study was conducted to determine the reference intervals for CD3+ (T cells), CD4+ ... more Objective-This study was conducted to determine the reference intervals for CD3+ (T cells), CD4+ (T helper/inducer cell, Th), CD8+ (T suppressor/cytotoxic cells, Ts/c), CD3+CD4+/ CD3+CD8+ ratio, CD19+ (B cells) and CD16++56+ (NK cells) in a healthy Iranian population. Method-Becton-Dickinson Immunocytometry Systems (BDIS) Simultest IMK-lymphocyte (dual color) reagents were used. The samples were first stained with appropriate monoclonal antibodies and then lysed using BDIS FACS lysing solution. The samples were analysed by BDIS FACS Caliber flowcytometry using the SimulSET software, version 2.5. Results- The study population consisted of 152 healthy, male and female non-smokers, 18 to 60 years of age. The mean CD3+ percentage was 69.95 % for males and 71.46 % for females, and the mean percentage of CD3+ CD4+ Th subset was 40.4 % and 44.21 % for males and females respectively. The mean CD3+ CD4+/CD3+ CD8+ ratio was 1.55±0.54 for male population and 1.7±0.65 for female populations. Th...

Research paper thumbnail of Increased activation and expansion of a CD57+ subset within peripheral CD8+ T lymphocytes in Mycobacterium tuberculosis-infected patients

Archives of Iranian medicine, 2006

BACKGROUND Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the ... more BACKGROUND Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of extracellular pathogens by limiting M.tuberculosis replication. Alterations in cytolytic function, inappropriate maturation/differentiation, and limited proliferation could reduce their ability to control M.tuberculosis replication. METHODS In an attempt to further characterize the immune responses during M.tuberculosis infection, we enumerated gamma-delta and alpha-beta receptor-bearing T cells expressing CD8 or CD4 phenotype and analyzed the differentiation phenotypes of CD8+ and CD4+ T lymphocyte subpopulations in 47 cases (23 new cases and 24 multidrug resistant patients) and 20 control subjects, using flowcytometry. RESULTS We found that the CD4/CD8 ratio was significantly lower in newly-diagnosed M.tuberculosis patients compared to multidrug resistant and control subjects (P < 0.003). Also, we found that a large proportion of CD8+ T lymphocytes in newly-diagnosed patie...

Research paper thumbnail of The immunomodulatory effects of low molecular weight garlic protein in crosstalk between peripheral blood mononuclear cells and colon cancer cells

Process Biochemistry, 2021

Garlic is an ancient plant whose medicinal usage has a long traditional history. It has been alwa... more Garlic is an ancient plant whose medicinal usage has a long traditional history. It has been always advocated as a suitable candidate for immunomodulation. The purpose of the present study was to assess the immunomodulatory effects of Low Molecular Weight Garlic Proteins (LMWGP) on the proliferation of peripheral blood mononuclear cells (PBMCs), induction of regulatory T cells (Tregs), as well as cytokine secretion in the co-culture of PBMCs and colon cancer cell lines, SW48. Garlic cloves were initially extracted and then protein fractions were separated using gel filtration chromatography and analyzed with mass spectrometry (MS). The effects of LMWGP on the proliferation of PBMCs and induction of Tregs were later analyzed by flow cytometry. Cytokine secretion was also evaluated using the ELISA test. The findings revealed that the proliferation of PBMCs had significantly augmented in the presence of LMWGP (p ≤ 0.05). Moreover, this fraction had reduced induction of Tregs in the co-...

Research paper thumbnail of Increased Activation and Expansion of a CD57+ Subset Within Peripheral CD8+ T Lymphocytes in Mycobacterium Tuberculosis-Infected Patients

Archives of Iranian Medicine, Feb 1, 2006

Background: Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the... more Background: Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of extracellular pathogens by limiting M.tuberculosis replication. Alterations in cytolytic function, inappropriate maturation/differentiation, and limited proliferation could reduce their ability to control M.tuberculosis replication. Methods: In an attempt to further characterize the immune responses during M.tuberculosis infection, we enumerated γδ and αβ receptor-bearing T cells expressing CD8 or CD4 phenotype and analyzed the differentiation phenotypes of CD8+ and CD4+ T lymphocyte subpopulations in 47 cases (23 new cases and 24 multidrug resistant patients) and 20 control subjects, using flowcytometry. Results: We found that the CD4/CD8 ratio was significantly lower in newly-diagnosed M.tuberculosis patients compared to multidrug resistant and control subjects (P < 0.003). Also, we found that a large proportion of CD8+ T lymphocytes in newly-diagnosed patients was defined by increased surface expression of CD57 as compared to the two other settings (P < 0.002). This increase was more profound in patients with an inverted CD4/CD8 ratio. Analysis of the late activation antigen revealed that this was predominantly HLA-DR+ (P < 0.003). No significant changes were observed in the percentages of CD8+CD57+ T cells between the different settings. Moreover, the co-stimulatory molecule CD28+ tended to be underexpressed by CD8+ T cells in multidrug resistant patients when compared to newly-diagnosed subjects (P < 0.002), but not to the control subjects. In contrast, the frequency of CD28+ marker on CD4+ T cells was higher in the setting of multidrug resistant compared with those of new cases (P < 0.0001). No significant changes were observed in percentages of γδ receptor-bearing T cells between different groups. Conclusion: We suggest that the increase in the proportion of CD57+ within CD8+ T cells in newly-diagnosed patients results from M.tuberculosis antigenic stimulation, which is a hallmark of many infections and that the protracted accumulation of CD57+ T lymphocytes might reflect an end-stage differentiation phenotype.

Research paper thumbnail of Increased activation and expansion of a CD57+ subset within peripheral CD8+ T lymphocytes in Mycobacterium tuberculosis-infected patients

Archives of Iranian medicine, 2006

Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of e... more Mycobacterium tuberculosis-specific CD8+ and CD4+ T lymphocyte responses restrict the spread of extracellular pathogens by limiting M.tuberculosis replication. Alterations in cytolytic function, inappropriate maturation/differentiation, and limited proliferation could reduce their ability to control M.tuberculosis replication. In an attempt to further characterize the immune responses during M.tuberculosis infection, we enumerated gamma-delta and alpha-beta receptor-bearing T cells expressing CD8 or CD4 phenotype and analyzed the differentiation phenotypes of CD8+ and CD4+ T lymphocyte subpopulations in 47 cases (23 new cases and 24 multidrug resistant patients) and 20 control subjects, using flowcytometry. We found that the CD4/CD8 ratio was significantly lower in newly-diagnosed M.tuberculosis patients compared to multidrug resistant and control subjects (P < 0.003). Also, we found that a large proportion of CD8+ T lymphocytes in newly-diagnosed patients was defined by increase...

Research paper thumbnail of Local eosinophils are associated with increased IgA subclass levels in the sinonasal mucosa of chronic rhinosinusitis with polyp patients

Allergy, Asthma & Clinical Immunology, Apr 25, 2020

Background: Chronic rhinosinusitis (CRS) describes an inflammatory condition affecting the sinona... more Background: Chronic rhinosinusitis (CRS) describes an inflammatory condition affecting the sinonasal mucosa. As the immune system players such as immunoglobulins play prominent roles in the development of CRS, we aimed to investigate the expression of IgA subclasses and factors involved in IgA class switching in the sinonasal mucosa of CRS patients. Methods: Specimens were collected from the sinonasal mucosa of the healthy controls and CRS patients. Histological assessments were performed by H&E and immunohistochemistry. Real-time PCR and ELISA methods were applied to measure gene expression and protein levels extracted from tissue samples, respectively. Results: We observed that total IgA and subclass-positive cells were higher in the patient groups than controls. There was a significant correlation between the number of eosinophils and total IgA and subclasses-positive cells (Pv < 0.0001). The expression of CXCL13, BAFF, AID, and germline transcripts were increased in CRSwNP patients. In contrast to IgA2 levels, IgA1 levels were significantly increased in the sinonasal tissue of CRSwNP patients (Pv < 0.01). TGF-β was significantly elevated in the sinonasal tissue of patients with CRSsNP. Conclusions: Increased protein levels of IgA subclasses and related antibody-producing cells were associated with elevated eosinophils in CRSwNP patients which may result in eosinophil pathological functions. Several therapeutic approaches might be developed to modulate the IgA production to ameliorate the inflammatory mechanisms in CRSwNP patients.

Research paper thumbnail of Levels of total IgA and IgA subclasses in the serum of chronic rhinosinusitis patients

Medical Journal of the Islamic Republic of Iran

Although IgE levels increase in the sera of patients with nasal polyps, and most studies have exa... more Although IgE levels increase in the sera of patients with nasal polyps, and most studies have examined its rate and role in these patients, IgA has not been studied as the most abundant antibody after IgG in sera of patients with CRS. →What this article adds: There is no significant difference in total IgA, IgA1, and IgA2 levels in serum of patients with CRS in comparison to healthy controls. Therefore, serum IgA is not a good marker for diagnosis and prognosis of CRS, consequently local investigation of these biomarkers may result in better interpretation.

Research paper thumbnail of Enumeration of Peripheral Blood Lymphocyte Subsets in a Healthy Iranian Population

Objective-This study was conducted to determine the reference intervals for CD3 + (T cells), CD4 ... more Objective-This study was conducted to determine the reference intervals for CD3 + (T cells), CD4 + (T helper/inducer cell, Th), CD8 + (T suppressor/cytotoxic cells, Ts/c), CD3 + CD4 + / CD3 + CD8 + ratio, CD19 + (B cells) and CD16 + +56 + (NK cells) in a healthy Iranian population. Method-Becton-Dickinson Immunocytometry Systems (BDIS) Simultest IMK-lymphocyte (dual color) reagents were used. The samples were first stained with appropriate monoclonal antibodies and then lysed using BDIS FACS lysing solution. The samples were analysed by BDIS FACS Caliber flowcytometry using the SimulSET software, version 2.5. Results-The study population consisted of 152 healthy, male and female non-smokers, 18 to 60 years of age. The mean CD3 + percentage was 69.95% for males and 71.46% for females, and the mean percentage of CD3 + CD4 + Th subset was 40.4% and 44.21% for males and females respectively. The mean CD3 + CD4 + /CD3 + CD8 + ratio was 1.55±0.54 for male population and 1.7±0.65 for female populations. The mean percentage of B lymphocytes (CD19 +) was 14.52% for the females and 13.08% for males. Conclusion-This study indicates that race, sex and age should be a consideration in the evaluation of the immune status.

Research paper thumbnail of T-Lymphocyte Subsets in Bronchoalveolar Lavage: A Flow-Cytometric Analysis and Its Importance in the Differential Diagnosis of Sarcoidosis

Background - The immunophenotyping of lymphocyte subsets in bronchoalveolar lavage (BAL) is of im... more Background - The immunophenotyping of lymphocyte subsets in bronchoalveolar lavage (BAL) is of importance in the differential diagnosis of interstitial lung diseases. The main intent for lymphocyte population subtyping in BAL fluid is finding those diseases with increased lymphocytic cell infiltration in the affected organ such as sarcoidosis and asbestosis. Methods - A dual color flow-cytometric analysis was performed to

Research paper thumbnail of 131 The role of T-cell subsets in burns patients

International Journal of Infectious Diseases, 2006