sri widia a jusman | Universitas Indonesia 5 (original) (raw)
Papers by sri widia a jusman
eJurnal kedokteran Indonesia, Sep 19, 2016
Acta Biochimica Indonesiana
Background: Maternal exposure to cigarette smoke during pregnancy and lactation might harmful for... more Background: Maternal exposure to cigarette smoke during pregnancy and lactation might harmful for the fetus. The smoke contains many free radicals that could be eliminated by antioxidant. This study aimed to investigate the effect of Spirulina platensis ethanol extract as antioxidant against cigarette smoke exposure during pregnancy until lactation by assessing oxidative stress markers in neonatal brain tissues. Methods: The experimental study used 26 offspring divided into four groups: (C) = offspring of maternal control group; (Cg) = offspring of maternal exposed to cigarette smoke; (CgSp) = offspring of maternal given spirulina and exposed to cigarette smoke; and (Sp) = offspring of maternal given spirulina only group, during gestation and 9 days lactation (30 days). Each group consisted of 6 offspring obtained from 2 adult females mated with male Sprague-Dawley rats. The exposure of cigarette smoke was 4 burn cigarettes/day for 30 days. The dose of extract was 200 mg/kg BW. The ...
AIP Conference Proceedings, Dec 31, 2023
Vitamin C (ascorbic acid) plays an exogenous antioxidant to protect biomolecules against cellular... more Vitamin C (ascorbic acid) plays an exogenous antioxidant to protect biomolecules against cellular redox-reactive species generation, including ROS, that is involved in the development of oxidative stress-related diseases. Therefore, some ascorbic acid studies have concentrated on its ROS scavenging activity. We designed a combination of vitamin C and octanoic acid as vitamin C derivatives to evaluate their antioxidant potential in silico study using molecular docking. Compound 1 demonstrated the receptor-ligand interaction in Lipoxygenase (LO) than Zileuton-ZIL with its lower binding free energy (ΔG) and inhibition constant (Ki) value. Meanwhile, 6 exhibited lower Gibs energy (ΔG) and inhibition constant (Ki) in NADPH oxidase (NO) receptor than Dextromethorphan-DEX. The results add to the evidence supporting antioxidant activities of 1 and 6 in two receptors (LO and NO, respectively) via ROS generation. Thus, further research is warranted into their efficiency in reducing oxidative stress for possible commercial antioxidants.
Journal of Retina-Vitreous, 2021
Journal of physics, Aug 1, 2017
Cancer stem cells have been reported to maintain stemness under certain extracellular changes. Th... more Cancer stem cells have been reported to maintain stemness under certain extracellular changes. This study aimed to analyze the effect of extracellular O2 level modulation on the glucose metabolism of human CD24-/CD44+ breast cancer stem cells (BCSCs). The primary BCSCs (CD24-/CD44+ cells) were cultured under hypoxia (1% O2) for 0.5, 4, 6, 24 and 48 hours. After each incubation period, HIF1α, GLUT1 and CA9 expressions, as well as glucose metabolism status, including glucose consumption, lactate production, O2 consumption and extracellular pH (pHe) were analyzed using qRT-PCR, colorimetry, fluorometry, and enzymatic reactions, respectively. Hypoxia caused an increase in HIF1α mRNA expressions and protein levels and shifted the metabolic states to anaerobic glycolysis, as demonstrated by increased glucose consumption and lactate production, as well as decreased O2 consumption and pHe. Furthermore, we demonstrated that GLUT1 and CA9 mRNA expressions simultaneously increased, in line with HIF1α expression. In conclusion, modulation of the extracellular environment of human BCSCs through hypoxia shifedt the metabolic state of BCSCs to anaerobic glycolysis, which might be associated with GLUT1 and CA9 expressions regulated by HIFlα transcription factor.
Annals of Oncology, Sep 1, 2017
Medical Journal of Indonesia, Feb 1, 2011
Jurnal Kedokteran Meditek, May 22, 2023
IJRETINA (International Journal of Retina), Feb 28, 2019
Annals of Oncology, Oct 1, 2018
eJournal Kedokteran Indonesia
The aim of the study is to examine the role of hypoxia-inducible factor-1a (HIF-1a) and intercell... more The aim of the study is to examine the role of hypoxia-inducible factor-1a (HIF-1a) and intercellular adhesive molecule (ICAM-1) in clinical findings found in patients with rhegmatogenous retinal detachment (RRD). The clinical findings are; the duration of retinal detachment, retinal quadrants involvement, and the severity of proliferative vitreoretinopathy (PVR). A cross sectional study was conducted, with 34 consecutive vitreous sample were obtained from dr. Cipto Mangunkusumo National General Hospital (RSCM), from October 2015 to October 2016. The samples were taken from RRD patient who received vitrectomy in the hospital. There were no significant difference between the ICAM-1 and HIF-1a levels to the clinical findings that is measured; duration of retinal detachment (p>0.05), extent of retinal detachment area (p>0.05) and severity of PVR (p>0.05). However, there is a significant mean difference of ICAM-1 level on the duration of retinal detachment, where < 1 week gr...
Frontiers in Endocrinology
BackgroundHyperthyroidism is related to vascular atherosclerosis. Propylthiouracil (PTU) and meth... more BackgroundHyperthyroidism is related to vascular atherosclerosis. Propylthiouracil (PTU) and methimazole, other than their antithyroid effects, may have different mechanisms in preventing atherogenesis in Graves’ disease.ObjectiveThis study aimed to investigate the effect of antithyroid drugs on markers of vascular atherosclerosis in Graves’ hyperthyroidism.MethodsThis study was a single-blind, randomized clinical trial conducted on 36 patients with Graves’ disease in Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, from June 2019 until July 2020. Graves’ disease was diagnosed from clinical manifestation of hyperthyroidism with diffuse goiter and then confirmed by thyroid stimulation hormone (TSH), free T4 (fT4), and TSH-receptor antibody (TRAb) measurements. Participants were randomly assigned to either a PTU or a methimazole treatment group and followed up for 3 months. Markers of vascular atherosclerosis were represented by adhesion molecules [intercellular adhesion molec...
International Journal of Human Movement and Sports Sciences
Futsal sports have high explosive characteristics with moderate-heavy intensity. The increase of ... more Futsal sports have high explosive characteristics with moderate-heavy intensity. The increase of cortisol is a biomarker for high-stress situations desirable throughout futsal-that catabolic hormone secreted in a state of physical or psychological stress. Meanwhile, a person's emotions are harmful, such as stress, anxiety, fear, and frustration. The release of the cortisol hormone can activate the sympathetic nervous system, distinguished by an increase in heart rate, and reduce the ability to think and react. Additionally, it represents a role in decreasing mood and muscle fatigue. This study's persistence was to determine the differences in the frequency of futsal practice on physical stress in adolescents (27 boys). This investigation approach is an experimental study in adolescents (13,63±0,84 years old) who perform futsal sports with different futsal frequency groups for ten weeks, specifically 1-time weekly exercise frequency (F1), 3-times weekly exercise frequency (F3), and 5-times weekly exercise frequency (F5). This research had passed the research ethics review issued. The testing results on F1 found no significant improvement in cortisol (51,21±22,66 vs. 61,02±27,69). In F3, there was no significant increase at the cortisol level (57,71±15,45 vs. 61,82±13,57), and in F5 group, there was a significant increase at the cortisol level (51,02±9,38 vs. 70,33±16,36). Interestingly, only the F5 group showed a significant increase at the cortisol level in this study. Anticipated F5 physically attends to be extremely high in the intensity of futsal exercise and induces fatigue and muscle damage that can stimulate an improvement in the endocrine system, constant with enhancing cortisol levels in the blood. This cortisol release can activate the sympathetic nervous system, characterized by a high pulse rate, but it can reduce the ability to think.
Jurusan Kebidanan Poltekkes Kemenkes Jakarta III, 2021
Indonesian journal of biotechnology and biodiversity, 2018
The oxygen need is very important in tissue regeneration process for the sustainability of metabo... more The oxygen need is very important in tissue regeneration process for the sustainability of metabolic process. The high activity of cells and tissues during regeneration requires high energy therefore oxygen demand is very high. Cytoglobin (Cygb) is a protein that plays a role to diffuse oxygen to mitochondria. Cygb is a molecule that has a high affinity to oxygen and expressed during hypoxic conditions, when the tissue lacks oxygen. The tissue in regeneration process experiences at hypoxia state, Cygb protein expression have an important role during the tissue regeneration process. The Spearman correlation test results the strong correlation between the expression of Cygb protein of immunoratio test in immunohistochemical staining with the growth of epidermal thickness of tissue (r = 0.67; p <0.05). The results of correlation test between Cygb protein expression with the growth of dermis thickness showed moderate correlation properties (r = 0.406; p <0.05).
![Research paper thumbnail of Effect of Lamun Leaves [Enhalus acoroides (L.f.) Royle] on Leydig Cell Morphology](https://attachments.academia-assets.com/110096643/thumbnails/1.jpg)
](ENDLESS : International Journal of Future Studies, 2021
The ageing male process lead deterioration fertilization ability that caused of Leydig cell degen... more The ageing male process lead deterioration fertilization ability that caused of Leydig cell degeneration by an accumulation of oxidative stress with decreased testosterone levels. Enhalus acoroides contains antioxidants and phytosterols which can be converted into testosterone. The aim of this study to investigate the extract of Enhalus acoroides effect on Leydig cell morphology in male mice. Male mice was divided into 8 groups: adult mice no treatment (AG1), adult mice with olive oil (AG2), adult mice with extract of Enhalus acoroides at dose 25 mgKg-1 (AG3), adult mice with extract of Enhalus acoroides at dose 50 mgKg-1 (AG4), old mice no treatment (OG1), old mice with olive oil (OG2), old mice with extract of Enhalus acoroides at dose 25 mg Kg-1 (OG3), and at dose 50 mgKg-1 (OG4). All groups were repeated for 14 days. There was a significantly increased number of old Leydig cells in old mice group and adult mice group at dose 25 mgKg-1 compared without treatment. There is no diff...
eJurnal kedokteran Indonesia, Sep 19, 2016
Acta Biochimica Indonesiana
Background: Maternal exposure to cigarette smoke during pregnancy and lactation might harmful for... more Background: Maternal exposure to cigarette smoke during pregnancy and lactation might harmful for the fetus. The smoke contains many free radicals that could be eliminated by antioxidant. This study aimed to investigate the effect of Spirulina platensis ethanol extract as antioxidant against cigarette smoke exposure during pregnancy until lactation by assessing oxidative stress markers in neonatal brain tissues. Methods: The experimental study used 26 offspring divided into four groups: (C) = offspring of maternal control group; (Cg) = offspring of maternal exposed to cigarette smoke; (CgSp) = offspring of maternal given spirulina and exposed to cigarette smoke; and (Sp) = offspring of maternal given spirulina only group, during gestation and 9 days lactation (30 days). Each group consisted of 6 offspring obtained from 2 adult females mated with male Sprague-Dawley rats. The exposure of cigarette smoke was 4 burn cigarettes/day for 30 days. The dose of extract was 200 mg/kg BW. The ...
AIP Conference Proceedings, Dec 31, 2023
Vitamin C (ascorbic acid) plays an exogenous antioxidant to protect biomolecules against cellular... more Vitamin C (ascorbic acid) plays an exogenous antioxidant to protect biomolecules against cellular redox-reactive species generation, including ROS, that is involved in the development of oxidative stress-related diseases. Therefore, some ascorbic acid studies have concentrated on its ROS scavenging activity. We designed a combination of vitamin C and octanoic acid as vitamin C derivatives to evaluate their antioxidant potential in silico study using molecular docking. Compound 1 demonstrated the receptor-ligand interaction in Lipoxygenase (LO) than Zileuton-ZIL with its lower binding free energy (ΔG) and inhibition constant (Ki) value. Meanwhile, 6 exhibited lower Gibs energy (ΔG) and inhibition constant (Ki) in NADPH oxidase (NO) receptor than Dextromethorphan-DEX. The results add to the evidence supporting antioxidant activities of 1 and 6 in two receptors (LO and NO, respectively) via ROS generation. Thus, further research is warranted into their efficiency in reducing oxidative stress for possible commercial antioxidants.
Journal of Retina-Vitreous, 2021
Journal of physics, Aug 1, 2017
Cancer stem cells have been reported to maintain stemness under certain extracellular changes. Th... more Cancer stem cells have been reported to maintain stemness under certain extracellular changes. This study aimed to analyze the effect of extracellular O2 level modulation on the glucose metabolism of human CD24-/CD44+ breast cancer stem cells (BCSCs). The primary BCSCs (CD24-/CD44+ cells) were cultured under hypoxia (1% O2) for 0.5, 4, 6, 24 and 48 hours. After each incubation period, HIF1α, GLUT1 and CA9 expressions, as well as glucose metabolism status, including glucose consumption, lactate production, O2 consumption and extracellular pH (pHe) were analyzed using qRT-PCR, colorimetry, fluorometry, and enzymatic reactions, respectively. Hypoxia caused an increase in HIF1α mRNA expressions and protein levels and shifted the metabolic states to anaerobic glycolysis, as demonstrated by increased glucose consumption and lactate production, as well as decreased O2 consumption and pHe. Furthermore, we demonstrated that GLUT1 and CA9 mRNA expressions simultaneously increased, in line with HIF1α expression. In conclusion, modulation of the extracellular environment of human BCSCs through hypoxia shifedt the metabolic state of BCSCs to anaerobic glycolysis, which might be associated with GLUT1 and CA9 expressions regulated by HIFlα transcription factor.
Annals of Oncology, Sep 1, 2017
Medical Journal of Indonesia, Feb 1, 2011
Jurnal Kedokteran Meditek, May 22, 2023
IJRETINA (International Journal of Retina), Feb 28, 2019
Annals of Oncology, Oct 1, 2018
eJournal Kedokteran Indonesia
The aim of the study is to examine the role of hypoxia-inducible factor-1a (HIF-1a) and intercell... more The aim of the study is to examine the role of hypoxia-inducible factor-1a (HIF-1a) and intercellular adhesive molecule (ICAM-1) in clinical findings found in patients with rhegmatogenous retinal detachment (RRD). The clinical findings are; the duration of retinal detachment, retinal quadrants involvement, and the severity of proliferative vitreoretinopathy (PVR). A cross sectional study was conducted, with 34 consecutive vitreous sample were obtained from dr. Cipto Mangunkusumo National General Hospital (RSCM), from October 2015 to October 2016. The samples were taken from RRD patient who received vitrectomy in the hospital. There were no significant difference between the ICAM-1 and HIF-1a levels to the clinical findings that is measured; duration of retinal detachment (p>0.05), extent of retinal detachment area (p>0.05) and severity of PVR (p>0.05). However, there is a significant mean difference of ICAM-1 level on the duration of retinal detachment, where < 1 week gr...
Frontiers in Endocrinology
BackgroundHyperthyroidism is related to vascular atherosclerosis. Propylthiouracil (PTU) and meth... more BackgroundHyperthyroidism is related to vascular atherosclerosis. Propylthiouracil (PTU) and methimazole, other than their antithyroid effects, may have different mechanisms in preventing atherogenesis in Graves’ disease.ObjectiveThis study aimed to investigate the effect of antithyroid drugs on markers of vascular atherosclerosis in Graves’ hyperthyroidism.MethodsThis study was a single-blind, randomized clinical trial conducted on 36 patients with Graves’ disease in Cipto Mangunkusumo General Hospital, Jakarta, Indonesia, from June 2019 until July 2020. Graves’ disease was diagnosed from clinical manifestation of hyperthyroidism with diffuse goiter and then confirmed by thyroid stimulation hormone (TSH), free T4 (fT4), and TSH-receptor antibody (TRAb) measurements. Participants were randomly assigned to either a PTU or a methimazole treatment group and followed up for 3 months. Markers of vascular atherosclerosis were represented by adhesion molecules [intercellular adhesion molec...
International Journal of Human Movement and Sports Sciences
Futsal sports have high explosive characteristics with moderate-heavy intensity. The increase of ... more Futsal sports have high explosive characteristics with moderate-heavy intensity. The increase of cortisol is a biomarker for high-stress situations desirable throughout futsal-that catabolic hormone secreted in a state of physical or psychological stress. Meanwhile, a person's emotions are harmful, such as stress, anxiety, fear, and frustration. The release of the cortisol hormone can activate the sympathetic nervous system, distinguished by an increase in heart rate, and reduce the ability to think and react. Additionally, it represents a role in decreasing mood and muscle fatigue. This study's persistence was to determine the differences in the frequency of futsal practice on physical stress in adolescents (27 boys). This investigation approach is an experimental study in adolescents (13,63±0,84 years old) who perform futsal sports with different futsal frequency groups for ten weeks, specifically 1-time weekly exercise frequency (F1), 3-times weekly exercise frequency (F3), and 5-times weekly exercise frequency (F5). This research had passed the research ethics review issued. The testing results on F1 found no significant improvement in cortisol (51,21±22,66 vs. 61,02±27,69). In F3, there was no significant increase at the cortisol level (57,71±15,45 vs. 61,82±13,57), and in F5 group, there was a significant increase at the cortisol level (51,02±9,38 vs. 70,33±16,36). Interestingly, only the F5 group showed a significant increase at the cortisol level in this study. Anticipated F5 physically attends to be extremely high in the intensity of futsal exercise and induces fatigue and muscle damage that can stimulate an improvement in the endocrine system, constant with enhancing cortisol levels in the blood. This cortisol release can activate the sympathetic nervous system, characterized by a high pulse rate, but it can reduce the ability to think.
Jurusan Kebidanan Poltekkes Kemenkes Jakarta III, 2021
Indonesian journal of biotechnology and biodiversity, 2018
The oxygen need is very important in tissue regeneration process for the sustainability of metabo... more The oxygen need is very important in tissue regeneration process for the sustainability of metabolic process. The high activity of cells and tissues during regeneration requires high energy therefore oxygen demand is very high. Cytoglobin (Cygb) is a protein that plays a role to diffuse oxygen to mitochondria. Cygb is a molecule that has a high affinity to oxygen and expressed during hypoxic conditions, when the tissue lacks oxygen. The tissue in regeneration process experiences at hypoxia state, Cygb protein expression have an important role during the tissue regeneration process. The Spearman correlation test results the strong correlation between the expression of Cygb protein of immunoratio test in immunohistochemical staining with the growth of epidermal thickness of tissue (r = 0.67; p <0.05). The results of correlation test between Cygb protein expression with the growth of dermis thickness showed moderate correlation properties (r = 0.406; p <0.05).
ENDLESS : International Journal of Future Studies, 2021
The ageing male process lead deterioration fertilization ability that caused of Leydig cell degen... more The ageing male process lead deterioration fertilization ability that caused of Leydig cell degeneration by an accumulation of oxidative stress with decreased testosterone levels. Enhalus acoroides contains antioxidants and phytosterols which can be converted into testosterone. The aim of this study to investigate the extract of Enhalus acoroides effect on Leydig cell morphology in male mice. Male mice was divided into 8 groups: adult mice no treatment (AG1), adult mice with olive oil (AG2), adult mice with extract of Enhalus acoroides at dose 25 mgKg-1 (AG3), adult mice with extract of Enhalus acoroides at dose 50 mgKg-1 (AG4), old mice no treatment (OG1), old mice with olive oil (OG2), old mice with extract of Enhalus acoroides at dose 25 mg Kg-1 (OG3), and at dose 50 mgKg-1 (OG4). All groups were repeated for 14 days. There was a significantly increased number of old Leydig cells in old mice group and adult mice group at dose 25 mgKg-1 compared without treatment. There is no diff...