Tatiana Zachepilo | Pavlov Institute of Physiology Russian Academy of Sciences (original) (raw)
Papers by Tatiana Zachepilo
Bulletin of Experimental Biology and Medicine, 2006
In rats with low excitability threshold of the nervous system demonstrating significant and persi... more In rats with low excitability threshold of the nervous system demonstrating significant and persistent behavioral disorders under stress conditions, the content of methylcytosine-binding protein MeCP2 in neuronal nuclei of hippocampal field CA3 decreased over 2 weeks after long-term emotional and pain stress. It was hypothesized that protein MeCP2 triggers epigenetic changes in DNA that underlie "stress memory".
AGU Fall Meeting Abstracts, Dec 1, 2019
This research is meant to analyze the influence of sales financing, profit share financing and No... more This research is meant to analyze the influence of sales financing, profit share financing and Non Performing Financing (NPF) to the profitability of Bank Umum Syariah which are measured by using Return on Equity (ROE). The population of this research is Bank Umum Syariah which are listed in Indonesia Stock Exchange during the periods of 2010 until 2014 with the quarterly financial data which has been obtained by using purposive sampling and based on the certain criteria which has been determined by the researcher, so that six Bank Umum Syariah which have met the criteria have been selected as samples. The criteria of sample collection is 1) Bank umum syariah which are listed in Bank Indonesia; 2) Bank umum syariah which have published consecutively their 2 nd quarterly financial report in 2010 until 4 th quarter in 2014; 3) Bank umum syariah which have complete data which is required in this research variable. The analysis technique has been done by using multiple linear regressions. Based on the result of the multiple linear regressions with its significance level is 5% so that this research shows that: 1) Sales financing variable has significant and positive influence to the profitability (ROE); 2) Profit Sharing Financing variable has negative and significant influence to the profitability; 3) Non Performing Financing (NPF) variable has negative and significant influence to the profitability (ROE).
International Journal of Molecular Sciences
Autophagy is a regulated mechanism of degradation of misfolded proteins and organelles in the cel... more Autophagy is a regulated mechanism of degradation of misfolded proteins and organelles in the cell. Neurons are highly differentiated cells with extended projections, and therefore, their functioning largely depends on the mechanisms of autophagy. For the first time in an animal model using immunohistochemistry, dot analysis, and qRT-PCR, the autophagy (macroautophagy) activity in neurons of two brain regions (hippocampus and neocortex) under normoxia and after exposure to hypoxia was studied. It was found that under normoxia, the autophagic activity was higher in the hippocampal neurons than in the neocortex of rats. In the hippocampus, the exposure of rats to hypoxia resulted in a decrease in the content of autophagy markers LC3 and p62, which was followed by activation of the autophagy-related gene expression. In the neocortex, no changes in these marker proteins were observed after the exposure to hypoxia. These data indicate that the neurons in the hippocampus and neocortex dif...
Integrative Physiology, 2020
Progress in the field of information technology and global wireless communications leads to chang... more Progress in the field of information technology and global wireless communications leads to changes in the natural electromagnetic background of the Earth and affects living organisms. Therefore, it is relevant to investigate the influence of technogenic and natural electromagnetic fields using model biological objects, including a honeybee, which is particularly sensitive to electromagnetic radiation (EMR). Current EMR protection systems that change the characteristics of electromagnetic radiation can also cause specific magneto-biological reactions in living systems. In this study we used the honeybee (Apis mellifera L.) to assess the effect of devices on the innate and cognitive components of the honeybee behavior. The devices included EMR resonators working in isolation and together with a WiFi router. We also changed (weakened/enhanced) the magnetic field relative to the Earth’s magnetic field at the experiment location. The experiments found that all the factors under study, e...
Russian Journal of Genetics, 2007
Methods of immunohistochemistry and fluorescent staining was used to study the localization and a... more Methods of immunohistochemistry and fluorescent staining was used to study the localization and amounts of protein components of the signal cascade connecting the receptor link (NMDA-subtype glutamate receptor) with actin of the cytoskeleton in the head ganglia of Drosophila strain CantonS (wild type, control) and strains carrying mutations vermilion, cinnabar , and cardinal , which sequentially inactivate tryptophanhydrolyzing enzymes during its metabolism into ommochrome. The obtained data are evidence for modulatory effects of genes controlling the kynurenine pathway of tryptophan metabolism on the major components of the signal cascade: the initial link (NMDA receptor, postsynaptic density protein-95, a structural protein involved in receptor localization and internalization), the intermediate link (limkinase-1, the key neuronal enzyme in actin remodeling) and the final link (f-actin, the critical factor in the morphogenesis of synaptic structures and, hence, in the processes of synaptic plasticity, learning and memory). It is suggested that kynurenine acid (an endogenous nonspecific antagonist of L-glutamate receptor) and 3-hydroxykynurenine capable of inducing a nonspecific stimulating effect are biochemical intermediates of the effects of these genes.
Molecular and cellular probes, Jun 1, 2017
We evaluated copy number variation (CNV) for four genes in rat strains differing in nervous syste... more We evaluated copy number variation (CNV) for four genes in rat strains differing in nervous system excitability. rpl13a copy number is significantly reduced in hippocampus and bone marrow in rats with a high excitability threshold and stress. The observed phenomenon may be associated with a role for rpl13a in lipid metabolism.
Morfologii͡a (Saint Petersburg, Russia), 2007
Localization NR1 and NR2 subunits of NMDA-receptor was studied in brain structures of the honeybe... more Localization NR1 and NR2 subunits of NMDA-receptor was studied in brain structures of the honeybee and Drosophila by immunohistochemistry. The Western-blotting and NR1 subunit antisense-knockdown confirmed specificity of antibodies to NR1 and NR2 subunits. The data obtained demonstrated similar distribution of NR1 and NR2 subunits of NMDA-receptor in the insect brain (cranial ganglion). The brain regions with the highest expression NR1 and NR2 were different in the honeybee and Drosophila. This can be associated with behavioral repertoire peculiarities in these insects.
The locations of the NR1 and NR2 subunits of the GABA receptor were studied in brain structures i... more The locations of the NR1 and NR2 subunits of the GABA receptor were studied in brain structures in insects--honeybees and fruit flies--using an immunohistochemical method. The specificities of the antibodies to the NR1 and NR2 subunits were confirmed by the antisense knockdown method for the NR1 subunit and western blotting. The data obtained here lead to the conclusion that the distributions of the NR1 and NR2 subunits of the NMDA receptor complex in the cerebral ganglia of the honeybee and fruit fly are similar; areas with the highest concentrations of NR1 and NR2 subunits were identified, and these were found to be different in the different insects. This is associated with the behavioral characteristics of these two insect species.
ABSTRACT Canton S strain) and Drosophila that had mutations in the structural genes of the enzyme... more ABSTRACT Canton S strain) and Drosophila that had mutations in the structural genes of the enzymes that successively block KMT stages [11]. We studied the mutations vermilion ( v ), which inactivates tryptophan dioxygenase (the absence of all kynurenines); cinnabar ( cn ), which inactivates kynurenine-3-hydroxylase (accumulation of kynurenine and kynurenic acid); and cardinal ( cd ), which inactivates phenoxazinone synthase (accumulation of 3-hydroxykynurenine). The data obtained are shown in the figure. It can be seen that LIMK-1 was found throughout the brain of wild-type Drosophila and achieved the highest concentration in the central complex (the ellipsoid and flabellar bodies, nodules), the higher center of locomotion which is also involved in learning [14, 15]. In contrast, the v mutant displayed uniform staining of all structures of the cerebral ganglion, which suggests that the enzyme activity decreased under the conditions of kynurenine deficit. The changes in the enzyme expression are associated with kynurenines, because the staining of the cerebral ganglion was normal in cd mutants and was more intense in cn mutants. The experiments were performed on five-day-old flies of both sexes (no sexrelated differences were found). To prepare brain slices (7 μ m), we took 20‐30 flies of each strain.
Neuroscience and Behavioral Physiology, 2011
Behavioral and histochemical studies demonstrated that endogenous tryptophan metabolites – kynure... more Behavioral and histochemical studies demonstrated that endogenous tryptophan metabolites – kynurenines – play a role in the long-term retention of memory traces and the functioning of key components in the (GluR–LIMK1– F-actin) signaling cascade, which mediates these functions. Kynurenine deficiency induced by injections of allopurinol (a tryptophan oxygenase inhibitor) inhibited long-term memory, decreased the level of expression of LIMK1, and
European Neuropsychopharmacology, 2014
ABSTRACT Stress can induce structural changes in the brain and contribute to a variety of chronic... more ABSTRACT Stress can induce structural changes in the brain and contribute to a variety of chronic diseases from post-traumatic stress disorder to depression. The hippocampus is a highly plastic brain region particularly susceptible to the effects of environmental stress. A genomic stress response partly consists in changes in insertion activity of transposable elements. Retrotransposons account for ~45% of the mammalian genome. Mechanisms of action of stress and the formation of posttraumatic stress disorder (PTSD) in hu-man are associated with retrotransposons (LINE1, L1) activity in various tissues. Endogenous retrotransposition of L1 elements has been proposed as one potential mechanism generating neuronal genome diversity. They are capable of inserting into new genomic locations, which can result in deleterious outcomes. The greatest interest is the study of these processes in the brain due to indi-vidual variability of neural processes to effective pharmacotherapy path finding. The purpose of this work was to investigate the effect of stress on the LINE1 insertion pattern in the hippocampus. We used the model of emotional stress for the PTSD-like state formation in two lines of rats with genetically determined differ-ences in the level of excitability of the nervous system (low and high) to study of emotionally painful stress (3 types: short-term, long-term and massive stress) effect on LINE1 retrotransposon insertion polymorphism in the dentate gyrus of the hippocampus. L1 insertions in the promoter of gene GRIN1 [glutamate receptor ionotropic N-methyl-D-aspartate (NMDA) subtype, subunit 1] examined by two-step PCR (general and directed). Glutamate NMDA receptors play a key role in synaptic plasticity, synap-togenesis, excitotoxicity, memory acquisition and learning. These receptors mediate neuronal functions in glutamate neurotransmis-sion. NMDAR-mediated neurotransmission in the hippocampus is implicated in cognitive and emotional disturbances during stress-related disorders. NMDA receptor subunit 1 is a core molecule in the NMDA receptor complex. GRIN1 expression is associated with neurotransmission efficiency and state of the nervous system. We suppose that L1 retrotransposon insertions to promoter of GRIN1 gene will alter GRIN1 expression. It was shown that L1 insertion pattern depends on the level of excitability of the nervous system, intensity and duration of stress exposure. The most variable spectrum of L1 transposable elements activity (L1 insertion pattern) is detected under the influence of massive stress in the rats with low excitability of the nervous system. We assume that L1 retrotransposon insertions take part in the regulation of expression of candidate genes associated with the stress response and the formation of psychopathology.
Zhurnal evoliutsionnoĭ biokhimii i fiziologii
Zhurnal evoliutsionnoĭ biokhimii i fiziologii
Zhurnal evoliutsionnoĭ biokhimii i fiziologii
Zhurnal evoliutsionnoĭ biokhimii i fiziologii
The work deals with study of character of localization in the honeybee head ganglion of metabotro... more The work deals with study of character of localization in the honeybee head ganglion of metabotropic receptor ImGluR5 and its role in memory formation. With aid of pharmacological method (injections of antisense oligonucleotide and of selective receptor agonist and antagonist) and of behavioral criterion (formation and testing of preservation in memory of conditioned alimentary reflex for olfactory stimulus), there is first shown participation of the studied receptor in formation of the honeybee long-term memory. By using the immunohistochemical method, there is first revealed the predominant expression of the ImGluR5 receptor in the mushroom body Canyon cells responsible for the insect integrative activity. The present study, together with the previous ones, allows concluding about the presence in the honeybee head ganglion of the group I of metabotropic glutamate receptors with two subtypes 1 and 5 (ImGluR1,5) that have similar with mammalian pharmacological properties favoring pr...
Bulletin of experimental biology and medicine, 2003
Pharmacological characteristics of non-NMDA receptors involved in associative learning were studi... more Pharmacological characteristics of non-NMDA receptors involved in associative learning were studied in wild honey bees (normal) and carriers of snow laranja mutation (kynurenine deficiency) by pharmacological analysis and behavioral criteria. The effects of systemic injections of non-NMDA receptor agonists (AMPA, kainic, quisqualic, and domoic acids), AMPA receptor antagonist (NS257-HCl), and AMPA receptor modulator (cyclothiaside) on retention of conditioned reflexes in short-term memory (1 min after the end of learning) were studied. The pharmacological characteristics of non-NMDA receptors were changed in snow laranja mutants with kynurenine deficiency.
Neuroscience and Behavioral Physiology, 2014
A review. Works related to research into the neuroanatomical basis of associative learning in the... more A review. Works related to research into the neuroanatomical basis of associative learning in the honeybee (proboscis extension reflex) are summarized. Data on the brain organization of the honeybee are presented. The parallel neural pathways of conditioned and unconditioned stimuli are shown. Contribution of different brain structures and identified neurons (VUMmx1, PE1) in the formation of the proboscis extension reflex are discussed.
Journal of Evolutionary Biochemistry and Physiology, 2004
ABSTRACT Participation of metabotropic glutamate receptors (mGluR) in associative learning of the... more ABSTRACT Participation of metabotropic glutamate receptors (mGluR) in associative learning of the honeybee Apis mellifera was studied in behavioral and electrophysiological experiments with use of pharmacological analysis. By the method of conditioned reflexes, effects of systemic injections of mGluR agonists and antagonists were studied on retention of elaborated conditioned reflexes in short-term and long-term memory. Injection of aminocyclopentandicarbonic acid (ACPD), ibotenate, and phosphoserine stimulated the memory in 3 h after the single learning procedure. The long- term memory was inhibited by mGluR antagonists — methylcarboxyphenylglycine and amino- phosphonopropionate as well as by non-competitive antagonists of mGluR1(CPCCOEt) and of mGluR5 (SIB 1757). Electrophysiological experiments demonstrated changes of characteristics of the action potential recorded from mushroom bodxies in response to stimulation of antennal lobes and during injections of mGluR III phosphoserine and the mGluR1 antagonist CPCCOEt. The obtained data allow suggesting participation of different groups of metabotropic glutamate receptors in the process of the honeybee associative learning.
Bulletin of Experimental Biology and Medicine, 2006
In rats with low excitability threshold of the nervous system demonstrating significant and persi... more In rats with low excitability threshold of the nervous system demonstrating significant and persistent behavioral disorders under stress conditions, the content of methylcytosine-binding protein MeCP2 in neuronal nuclei of hippocampal field CA3 decreased over 2 weeks after long-term emotional and pain stress. It was hypothesized that protein MeCP2 triggers epigenetic changes in DNA that underlie "stress memory".
AGU Fall Meeting Abstracts, Dec 1, 2019
This research is meant to analyze the influence of sales financing, profit share financing and No... more This research is meant to analyze the influence of sales financing, profit share financing and Non Performing Financing (NPF) to the profitability of Bank Umum Syariah which are measured by using Return on Equity (ROE). The population of this research is Bank Umum Syariah which are listed in Indonesia Stock Exchange during the periods of 2010 until 2014 with the quarterly financial data which has been obtained by using purposive sampling and based on the certain criteria which has been determined by the researcher, so that six Bank Umum Syariah which have met the criteria have been selected as samples. The criteria of sample collection is 1) Bank umum syariah which are listed in Bank Indonesia; 2) Bank umum syariah which have published consecutively their 2 nd quarterly financial report in 2010 until 4 th quarter in 2014; 3) Bank umum syariah which have complete data which is required in this research variable. The analysis technique has been done by using multiple linear regressions. Based on the result of the multiple linear regressions with its significance level is 5% so that this research shows that: 1) Sales financing variable has significant and positive influence to the profitability (ROE); 2) Profit Sharing Financing variable has negative and significant influence to the profitability; 3) Non Performing Financing (NPF) variable has negative and significant influence to the profitability (ROE).
International Journal of Molecular Sciences
Autophagy is a regulated mechanism of degradation of misfolded proteins and organelles in the cel... more Autophagy is a regulated mechanism of degradation of misfolded proteins and organelles in the cell. Neurons are highly differentiated cells with extended projections, and therefore, their functioning largely depends on the mechanisms of autophagy. For the first time in an animal model using immunohistochemistry, dot analysis, and qRT-PCR, the autophagy (macroautophagy) activity in neurons of two brain regions (hippocampus and neocortex) under normoxia and after exposure to hypoxia was studied. It was found that under normoxia, the autophagic activity was higher in the hippocampal neurons than in the neocortex of rats. In the hippocampus, the exposure of rats to hypoxia resulted in a decrease in the content of autophagy markers LC3 and p62, which was followed by activation of the autophagy-related gene expression. In the neocortex, no changes in these marker proteins were observed after the exposure to hypoxia. These data indicate that the neurons in the hippocampus and neocortex dif...
Integrative Physiology, 2020
Progress in the field of information technology and global wireless communications leads to chang... more Progress in the field of information technology and global wireless communications leads to changes in the natural electromagnetic background of the Earth and affects living organisms. Therefore, it is relevant to investigate the influence of technogenic and natural electromagnetic fields using model biological objects, including a honeybee, which is particularly sensitive to electromagnetic radiation (EMR). Current EMR protection systems that change the characteristics of electromagnetic radiation can also cause specific magneto-biological reactions in living systems. In this study we used the honeybee (Apis mellifera L.) to assess the effect of devices on the innate and cognitive components of the honeybee behavior. The devices included EMR resonators working in isolation and together with a WiFi router. We also changed (weakened/enhanced) the magnetic field relative to the Earth’s magnetic field at the experiment location. The experiments found that all the factors under study, e...
Russian Journal of Genetics, 2007
Methods of immunohistochemistry and fluorescent staining was used to study the localization and a... more Methods of immunohistochemistry and fluorescent staining was used to study the localization and amounts of protein components of the signal cascade connecting the receptor link (NMDA-subtype glutamate receptor) with actin of the cytoskeleton in the head ganglia of Drosophila strain CantonS (wild type, control) and strains carrying mutations vermilion, cinnabar , and cardinal , which sequentially inactivate tryptophanhydrolyzing enzymes during its metabolism into ommochrome. The obtained data are evidence for modulatory effects of genes controlling the kynurenine pathway of tryptophan metabolism on the major components of the signal cascade: the initial link (NMDA receptor, postsynaptic density protein-95, a structural protein involved in receptor localization and internalization), the intermediate link (limkinase-1, the key neuronal enzyme in actin remodeling) and the final link (f-actin, the critical factor in the morphogenesis of synaptic structures and, hence, in the processes of synaptic plasticity, learning and memory). It is suggested that kynurenine acid (an endogenous nonspecific antagonist of L-glutamate receptor) and 3-hydroxykynurenine capable of inducing a nonspecific stimulating effect are biochemical intermediates of the effects of these genes.
Molecular and cellular probes, Jun 1, 2017
We evaluated copy number variation (CNV) for four genes in rat strains differing in nervous syste... more We evaluated copy number variation (CNV) for four genes in rat strains differing in nervous system excitability. rpl13a copy number is significantly reduced in hippocampus and bone marrow in rats with a high excitability threshold and stress. The observed phenomenon may be associated with a role for rpl13a in lipid metabolism.
Morfologii͡a (Saint Petersburg, Russia), 2007
Localization NR1 and NR2 subunits of NMDA-receptor was studied in brain structures of the honeybe... more Localization NR1 and NR2 subunits of NMDA-receptor was studied in brain structures of the honeybee and Drosophila by immunohistochemistry. The Western-blotting and NR1 subunit antisense-knockdown confirmed specificity of antibodies to NR1 and NR2 subunits. The data obtained demonstrated similar distribution of NR1 and NR2 subunits of NMDA-receptor in the insect brain (cranial ganglion). The brain regions with the highest expression NR1 and NR2 were different in the honeybee and Drosophila. This can be associated with behavioral repertoire peculiarities in these insects.
The locations of the NR1 and NR2 subunits of the GABA receptor were studied in brain structures i... more The locations of the NR1 and NR2 subunits of the GABA receptor were studied in brain structures in insects--honeybees and fruit flies--using an immunohistochemical method. The specificities of the antibodies to the NR1 and NR2 subunits were confirmed by the antisense knockdown method for the NR1 subunit and western blotting. The data obtained here lead to the conclusion that the distributions of the NR1 and NR2 subunits of the NMDA receptor complex in the cerebral ganglia of the honeybee and fruit fly are similar; areas with the highest concentrations of NR1 and NR2 subunits were identified, and these were found to be different in the different insects. This is associated with the behavioral characteristics of these two insect species.
ABSTRACT Canton S strain) and Drosophila that had mutations in the structural genes of the enzyme... more ABSTRACT Canton S strain) and Drosophila that had mutations in the structural genes of the enzymes that successively block KMT stages [11]. We studied the mutations vermilion ( v ), which inactivates tryptophan dioxygenase (the absence of all kynurenines); cinnabar ( cn ), which inactivates kynurenine-3-hydroxylase (accumulation of kynurenine and kynurenic acid); and cardinal ( cd ), which inactivates phenoxazinone synthase (accumulation of 3-hydroxykynurenine). The data obtained are shown in the figure. It can be seen that LIMK-1 was found throughout the brain of wild-type Drosophila and achieved the highest concentration in the central complex (the ellipsoid and flabellar bodies, nodules), the higher center of locomotion which is also involved in learning [14, 15]. In contrast, the v mutant displayed uniform staining of all structures of the cerebral ganglion, which suggests that the enzyme activity decreased under the conditions of kynurenine deficit. The changes in the enzyme expression are associated with kynurenines, because the staining of the cerebral ganglion was normal in cd mutants and was more intense in cn mutants. The experiments were performed on five-day-old flies of both sexes (no sexrelated differences were found). To prepare brain slices (7 μ m), we took 20‐30 flies of each strain.
Neuroscience and Behavioral Physiology, 2011
Behavioral and histochemical studies demonstrated that endogenous tryptophan metabolites – kynure... more Behavioral and histochemical studies demonstrated that endogenous tryptophan metabolites – kynurenines – play a role in the long-term retention of memory traces and the functioning of key components in the (GluR–LIMK1– F-actin) signaling cascade, which mediates these functions. Kynurenine deficiency induced by injections of allopurinol (a tryptophan oxygenase inhibitor) inhibited long-term memory, decreased the level of expression of LIMK1, and
European Neuropsychopharmacology, 2014
ABSTRACT Stress can induce structural changes in the brain and contribute to a variety of chronic... more ABSTRACT Stress can induce structural changes in the brain and contribute to a variety of chronic diseases from post-traumatic stress disorder to depression. The hippocampus is a highly plastic brain region particularly susceptible to the effects of environmental stress. A genomic stress response partly consists in changes in insertion activity of transposable elements. Retrotransposons account for ~45% of the mammalian genome. Mechanisms of action of stress and the formation of posttraumatic stress disorder (PTSD) in hu-man are associated with retrotransposons (LINE1, L1) activity in various tissues. Endogenous retrotransposition of L1 elements has been proposed as one potential mechanism generating neuronal genome diversity. They are capable of inserting into new genomic locations, which can result in deleterious outcomes. The greatest interest is the study of these processes in the brain due to indi-vidual variability of neural processes to effective pharmacotherapy path finding. The purpose of this work was to investigate the effect of stress on the LINE1 insertion pattern in the hippocampus. We used the model of emotional stress for the PTSD-like state formation in two lines of rats with genetically determined differ-ences in the level of excitability of the nervous system (low and high) to study of emotionally painful stress (3 types: short-term, long-term and massive stress) effect on LINE1 retrotransposon insertion polymorphism in the dentate gyrus of the hippocampus. L1 insertions in the promoter of gene GRIN1 [glutamate receptor ionotropic N-methyl-D-aspartate (NMDA) subtype, subunit 1] examined by two-step PCR (general and directed). Glutamate NMDA receptors play a key role in synaptic plasticity, synap-togenesis, excitotoxicity, memory acquisition and learning. These receptors mediate neuronal functions in glutamate neurotransmis-sion. NMDAR-mediated neurotransmission in the hippocampus is implicated in cognitive and emotional disturbances during stress-related disorders. NMDA receptor subunit 1 is a core molecule in the NMDA receptor complex. GRIN1 expression is associated with neurotransmission efficiency and state of the nervous system. We suppose that L1 retrotransposon insertions to promoter of GRIN1 gene will alter GRIN1 expression. It was shown that L1 insertion pattern depends on the level of excitability of the nervous system, intensity and duration of stress exposure. The most variable spectrum of L1 transposable elements activity (L1 insertion pattern) is detected under the influence of massive stress in the rats with low excitability of the nervous system. We assume that L1 retrotransposon insertions take part in the regulation of expression of candidate genes associated with the stress response and the formation of psychopathology.
Zhurnal evoliutsionnoĭ biokhimii i fiziologii
Zhurnal evoliutsionnoĭ biokhimii i fiziologii
Zhurnal evoliutsionnoĭ biokhimii i fiziologii
Zhurnal evoliutsionnoĭ biokhimii i fiziologii
The work deals with study of character of localization in the honeybee head ganglion of metabotro... more The work deals with study of character of localization in the honeybee head ganglion of metabotropic receptor ImGluR5 and its role in memory formation. With aid of pharmacological method (injections of antisense oligonucleotide and of selective receptor agonist and antagonist) and of behavioral criterion (formation and testing of preservation in memory of conditioned alimentary reflex for olfactory stimulus), there is first shown participation of the studied receptor in formation of the honeybee long-term memory. By using the immunohistochemical method, there is first revealed the predominant expression of the ImGluR5 receptor in the mushroom body Canyon cells responsible for the insect integrative activity. The present study, together with the previous ones, allows concluding about the presence in the honeybee head ganglion of the group I of metabotropic glutamate receptors with two subtypes 1 and 5 (ImGluR1,5) that have similar with mammalian pharmacological properties favoring pr...
Bulletin of experimental biology and medicine, 2003
Pharmacological characteristics of non-NMDA receptors involved in associative learning were studi... more Pharmacological characteristics of non-NMDA receptors involved in associative learning were studied in wild honey bees (normal) and carriers of snow laranja mutation (kynurenine deficiency) by pharmacological analysis and behavioral criteria. The effects of systemic injections of non-NMDA receptor agonists (AMPA, kainic, quisqualic, and domoic acids), AMPA receptor antagonist (NS257-HCl), and AMPA receptor modulator (cyclothiaside) on retention of conditioned reflexes in short-term memory (1 min after the end of learning) were studied. The pharmacological characteristics of non-NMDA receptors were changed in snow laranja mutants with kynurenine deficiency.
Neuroscience and Behavioral Physiology, 2014
A review. Works related to research into the neuroanatomical basis of associative learning in the... more A review. Works related to research into the neuroanatomical basis of associative learning in the honeybee (proboscis extension reflex) are summarized. Data on the brain organization of the honeybee are presented. The parallel neural pathways of conditioned and unconditioned stimuli are shown. Contribution of different brain structures and identified neurons (VUMmx1, PE1) in the formation of the proboscis extension reflex are discussed.
Journal of Evolutionary Biochemistry and Physiology, 2004
ABSTRACT Participation of metabotropic glutamate receptors (mGluR) in associative learning of the... more ABSTRACT Participation of metabotropic glutamate receptors (mGluR) in associative learning of the honeybee Apis mellifera was studied in behavioral and electrophysiological experiments with use of pharmacological analysis. By the method of conditioned reflexes, effects of systemic injections of mGluR agonists and antagonists were studied on retention of elaborated conditioned reflexes in short-term and long-term memory. Injection of aminocyclopentandicarbonic acid (ACPD), ibotenate, and phosphoserine stimulated the memory in 3 h after the single learning procedure. The long- term memory was inhibited by mGluR antagonists — methylcarboxyphenylglycine and amino- phosphonopropionate as well as by non-competitive antagonists of mGluR1(CPCCOEt) and of mGluR5 (SIB 1757). Electrophysiological experiments demonstrated changes of characteristics of the action potential recorded from mushroom bodxies in response to stimulation of antennal lobes and during injections of mGluR III phosphoserine and the mGluR1 antagonist CPCCOEt. The obtained data allow suggesting participation of different groups of metabotropic glutamate receptors in the process of the honeybee associative learning.