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Papers by Fatma Bilge EMRE

Research paper thumbnail of Fetemm Eği̇ti̇mi̇ni̇n Öğrenci̇leri̇n Fen Bi̇li̇mleri̇ Dersi̇ Başarisi Üzeri̇ne Etki̇li̇li̇ği̇: Bi̇r Meta Anali̇z Çalişmasi

The Journal of Academic Social Science Studies, 2021

Research paper thumbnail of Preparation, Characterization and H2O2 Selectivity of Hyperbranched Polyimides Containing Triazine

Journal of Polymer Research, 2005

Novel polyimides based on aromatic dianhyride and various hexahydrotriazine monomers were synthes... more Novel polyimides based on aromatic dianhyride and various hexahydrotriazine monomers were synthesized via two-stage solution polycondensation method. The resulting polyimides were characterized by solubility, viscosity, density, spectroscopic and thermal analysis ...

Research paper thumbnail of A benzimidazole-based conducting polymer and a PMMA–clay nanocomposite containing biosensor platform for glucose sensing

Synthetic Metals, 2015

ABSTRACT Development of materials composed of polymer–clay nanocomposites (PCN) and conducting po... more ABSTRACT Development of materials composed of polymer–clay nanocomposites (PCN) and conducting polymers attracts great interest and preferred in various applications. Hereby, polymethylmethacrylate (PMMA) layered silicate nanocomposites were prepared by in-situ suspension polymerization by grafting PMMA with laponite using a suitable grafting agent. The properties of the as-synthesized PCN materials are characterized by differential scanning calorimetry (DSC), thermal gravimetry analysis (TGA) and gel permeation chromatography (GPC). A conducting polymer; poly(4-(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-7-(2,3-dihydrothieno[3,4-b][1,4]dioxin-7-yl)-2-benzyl-1H-benzo[d]imidazole) (poly(BIPE)) and a PMMA–clay nanocomposite with 2-(methacryloyloxy) ethyltrimethylammonium chloride (MTMA) modifier were examined as a platform for biomolecule deposition. Glucose oxidase (GOx, β-d-glucose: oxygen-1-oxidoreductase, EC 1.1.3.4) was chosen as the model enzyme to prepare a scaffold for glucose sensing. Three different sensing strategies; PCN/GOx, poly(BIPE)/GOx and PCN/poly(BIPE)/GOx were analyzed and their biosensor performances were discussed. Surface morphology of the modified electrodes was characterized by scanning electron microscopy (SEM) technique. Electrochemical responses of the enzyme electrodes were monitored at −0.7 V vs. Ag reference electrode by monitoring oxygen consumption in the presence of glucose. After optimum conditions were determined, kinetic and analytical parameters; KMapp, Imax, LOD and sensitivity were investigated for each sensing platform.

Research paper thumbnail of Implications of FAD Electrode Reaction Kinetics for Electrocatalysis of NADH Oxidation and Development of NAD-Dependent Enzyme Electrodes

Electroanalysis, 2014

ABSTRACT FAD entrapped into the polyethyleneimine (PEI) matrix underwent 2e−/2H+ redox transforma... more ABSTRACT FAD entrapped into the polyethyleneimine (PEI) matrix underwent 2e−/2H+ redox transformation, in contrast to the 1e−/1H+ reaction observed with non-stabilized FAD-modified electrodes. Under conditions of the 2e−/2H+ reaction, FAD/PEI-modified electrodes catalytically oxidized NADH starting from −100 mV vs. Ag/AgCl. These electrodes may be used for construction of NAD-dependent dehydrogenase electrodes for clinically important analytes such as glucose when coupled to the model enzyme glucose dehydrogenase. Latter allows detection of glucose starting from −100 mV within 0.05–5 mM concentration range. These results suggest the FAD stabilization by different matrices as a simple and promising approach to achieve its 2e−/2H+ redox chemistry resulting in catalysis of NADH oxidation.

Research paper thumbnail of Effect of nanosized TiO2 particles on the development of Xenopus laevis embryos

TURKISH JOURNAL OF BIOLOGY, 2014

This paper reports the toxic properties of nano-TiO 2 on 2 different early life stages of Xenopus... more This paper reports the toxic properties of nano-TiO 2 on 2 different early life stages of Xenopus laevis. Synthesis of nano-TiO 2 particles was carried out by the hydrothermal method. Characterization of nanoparticles was performed using spectral techniques including X-ray diffraction, scanning electron microscopy, and a particle-size analyzer. Embryos at the 8th stage and tadpoles at the 46th stage were exposed to 7 concentrations of nano-TiO 2 in the range of 5 to 320 ppm. After 96 h of exposure, the mortality percentage of each exposure concentration was calculated and the activity of enzyme biomarkers acetylcholinesterase, carboxylesterase, glutathione S-transferase, glutathione reductase, lactate dehydrogenase, and aspartate aminotransferase were determined in living embryos and tadpoles. None of the tested concentrations of TiO 2 caused statistically significant mortality or malformation (only for the embryo test) as compared to the control groups. Furthermore, we did not observe any significant changes in enzyme activities in tadpole samples from the 46th stage, although some minor changes not related to the concentrations were observed in embryos.

Research paper thumbnail of Removal of Inorganic–Organic Bound Cu(II) from Different Aqueous Solutions by New Adsorbents Synthesized by Sol–Gel Process

Separation Science and Technology, 2005

Hydrolysis product of alcohol modified titanium(IV)‐n‐propoxide and its coated form were prepared... more Hydrolysis product of alcohol modified titanium(IV)‐n‐propoxide and its coated form were prepared as new and regenerable adsorbents for adsorbing and removing Cu(II) ions from its different aqueous solutions. The hydrolysis and alcoholysis products and adsorbents were characterized using GC, H‐NMR, FT‐IR, SEM, and TG‐DTA analysis. It was found that Cu(II) ion adsorption rather increased when hydrolysis product of alcohol modified

Research paper thumbnail of Conducting polymers with benzothiadiazole and benzoselenadiazole units for biosensor applications

Sensors and Actuators B: Chemical, 2011

Poly(4,7-di(2,3)-dihydrothienol [3,4-b][1,4]dioxin-5-yl-benzo[1,2,5]thiadiazole) (PBDT) and poly(... more Poly(4,7-di(2,3)-dihydrothienol [3,4-b][1,4]dioxin-5-yl-benzo[1,2,5]thiadiazole) (PBDT) and poly(4,7di(2,3)-dihydrothienol [3,4-b][1,4]dioxin-5-yl-2,1,3-benzoselenadiazole) (PESeE) were electrochemically deposited on graphite electrodes and used as immobilization matrices for biosensing studies. After electrochemical deposition of the polymeric matrices, glucose oxidase (GOx) was immobilized on the modified electrodes as the model enzyme. In the biosensing studies, the decrease in oxygen level as a result of enzymatic reaction was monitored at −0.7 V vs Ag/AgCl (3.0 M KCl) and correlated with substrate concentration. The biosensor was characterized in terms of several parameters such as operational and storage stabilities, kinetic parameters (K m and I max ) and surface morphologies. The biosensor was tested on real human blood serum samples.

Research paper thumbnail of Functionalization of poly-SNS-anchored carboxylic acid with Lys and PAMAM: surface modifications for biomolecule immobilization/stabilization and bio-sensing applications

The Analyst, 2012

Poly(2-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl) (SNS) acetic acid) was electrochemically deposited ... more Poly(2-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl) (SNS) acetic acid) was electrochemically deposited on graphite electrodes and functionalized with lysine (Lys) amino acid and poly(amidoamine) derivatives (PAMAM G2 and PAMAM G4) to investigate their matrix properties for biosensor applications. Glucose oxidase (GOx) was immobilized onto the modified surface as the model enzyme. X-Ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) were used to report the surface properties of the matrices in each step of the biosensor construction. The biosensors were characterized in terms of their operational and storage stabilities and the kinetic parameters (K app m and I max ). Three new glucose biosensors revealed good stability, featuring low detection limits (19.0 mM, 3.47 mM and 2.93 mM for lysine-, PAMAM G2-and PAMAM G4-functionalized electrodes, respectively) and prolonged the shelf lives (4, 5, and 6 weeks for Lys-, PAMAM G2-and PAMAM G4-modified electrodes, respectively). The proposed biosensors were tested for glucose detection on real human blood serum samples.

Research paper thumbnail of Preparation, Characterization and H2O2 Selectivity of Hyperbranched Polyimides Containing Triazine

Journal of Polymer Research, 2005

Novel polyimides based on aromatic dianhyride and various hexahydrotriazine monomers were synthes... more Novel polyimides based on aromatic dianhyride and various hexahydrotriazine monomers were synthesized via two-stage solution polycondensation method. The resulting polyimides were characterized by solubility, viscosity, density, spectroscopic and thermal analysis ...

Research paper thumbnail of Fetemm Eği̇ti̇mi̇ni̇n Öğrenci̇leri̇n Fen Bi̇li̇mleri̇ Dersi̇ Başarisi Üzeri̇ne Etki̇li̇li̇ği̇: Bi̇r Meta Anali̇z Çalişmasi

The Journal of Academic Social Science Studies, 2021

Research paper thumbnail of Preparation, Characterization and H2O2 Selectivity of Hyperbranched Polyimides Containing Triazine

Journal of Polymer Research, 2005

Novel polyimides based on aromatic dianhyride and various hexahydrotriazine monomers were synthes... more Novel polyimides based on aromatic dianhyride and various hexahydrotriazine monomers were synthesized via two-stage solution polycondensation method. The resulting polyimides were characterized by solubility, viscosity, density, spectroscopic and thermal analysis ...

Research paper thumbnail of A benzimidazole-based conducting polymer and a PMMA–clay nanocomposite containing biosensor platform for glucose sensing

Synthetic Metals, 2015

ABSTRACT Development of materials composed of polymer–clay nanocomposites (PCN) and conducting po... more ABSTRACT Development of materials composed of polymer–clay nanocomposites (PCN) and conducting polymers attracts great interest and preferred in various applications. Hereby, polymethylmethacrylate (PMMA) layered silicate nanocomposites were prepared by in-situ suspension polymerization by grafting PMMA with laponite using a suitable grafting agent. The properties of the as-synthesized PCN materials are characterized by differential scanning calorimetry (DSC), thermal gravimetry analysis (TGA) and gel permeation chromatography (GPC). A conducting polymer; poly(4-(2,3-dihydrothieno[3,4-b][1,4]dioxin-5-yl)-7-(2,3-dihydrothieno[3,4-b][1,4]dioxin-7-yl)-2-benzyl-1H-benzo[d]imidazole) (poly(BIPE)) and a PMMA–clay nanocomposite with 2-(methacryloyloxy) ethyltrimethylammonium chloride (MTMA) modifier were examined as a platform for biomolecule deposition. Glucose oxidase (GOx, β-d-glucose: oxygen-1-oxidoreductase, EC 1.1.3.4) was chosen as the model enzyme to prepare a scaffold for glucose sensing. Three different sensing strategies; PCN/GOx, poly(BIPE)/GOx and PCN/poly(BIPE)/GOx were analyzed and their biosensor performances were discussed. Surface morphology of the modified electrodes was characterized by scanning electron microscopy (SEM) technique. Electrochemical responses of the enzyme electrodes were monitored at −0.7 V vs. Ag reference electrode by monitoring oxygen consumption in the presence of glucose. After optimum conditions were determined, kinetic and analytical parameters; KMapp, Imax, LOD and sensitivity were investigated for each sensing platform.

Research paper thumbnail of Implications of FAD Electrode Reaction Kinetics for Electrocatalysis of NADH Oxidation and Development of NAD-Dependent Enzyme Electrodes

Electroanalysis, 2014

ABSTRACT FAD entrapped into the polyethyleneimine (PEI) matrix underwent 2e−/2H+ redox transforma... more ABSTRACT FAD entrapped into the polyethyleneimine (PEI) matrix underwent 2e−/2H+ redox transformation, in contrast to the 1e−/1H+ reaction observed with non-stabilized FAD-modified electrodes. Under conditions of the 2e−/2H+ reaction, FAD/PEI-modified electrodes catalytically oxidized NADH starting from −100 mV vs. Ag/AgCl. These electrodes may be used for construction of NAD-dependent dehydrogenase electrodes for clinically important analytes such as glucose when coupled to the model enzyme glucose dehydrogenase. Latter allows detection of glucose starting from −100 mV within 0.05–5 mM concentration range. These results suggest the FAD stabilization by different matrices as a simple and promising approach to achieve its 2e−/2H+ redox chemistry resulting in catalysis of NADH oxidation.

Research paper thumbnail of Effect of nanosized TiO2 particles on the development of Xenopus laevis embryos

TURKISH JOURNAL OF BIOLOGY, 2014

This paper reports the toxic properties of nano-TiO 2 on 2 different early life stages of Xenopus... more This paper reports the toxic properties of nano-TiO 2 on 2 different early life stages of Xenopus laevis. Synthesis of nano-TiO 2 particles was carried out by the hydrothermal method. Characterization of nanoparticles was performed using spectral techniques including X-ray diffraction, scanning electron microscopy, and a particle-size analyzer. Embryos at the 8th stage and tadpoles at the 46th stage were exposed to 7 concentrations of nano-TiO 2 in the range of 5 to 320 ppm. After 96 h of exposure, the mortality percentage of each exposure concentration was calculated and the activity of enzyme biomarkers acetylcholinesterase, carboxylesterase, glutathione S-transferase, glutathione reductase, lactate dehydrogenase, and aspartate aminotransferase were determined in living embryos and tadpoles. None of the tested concentrations of TiO 2 caused statistically significant mortality or malformation (only for the embryo test) as compared to the control groups. Furthermore, we did not observe any significant changes in enzyme activities in tadpole samples from the 46th stage, although some minor changes not related to the concentrations were observed in embryos.

Research paper thumbnail of Removal of Inorganic–Organic Bound Cu(II) from Different Aqueous Solutions by New Adsorbents Synthesized by Sol–Gel Process

Separation Science and Technology, 2005

Hydrolysis product of alcohol modified titanium(IV)‐n‐propoxide and its coated form were prepared... more Hydrolysis product of alcohol modified titanium(IV)‐n‐propoxide and its coated form were prepared as new and regenerable adsorbents for adsorbing and removing Cu(II) ions from its different aqueous solutions. The hydrolysis and alcoholysis products and adsorbents were characterized using GC, H‐NMR, FT‐IR, SEM, and TG‐DTA analysis. It was found that Cu(II) ion adsorption rather increased when hydrolysis product of alcohol modified

Research paper thumbnail of Conducting polymers with benzothiadiazole and benzoselenadiazole units for biosensor applications

Sensors and Actuators B: Chemical, 2011

Poly(4,7-di(2,3)-dihydrothienol [3,4-b][1,4]dioxin-5-yl-benzo[1,2,5]thiadiazole) (PBDT) and poly(... more Poly(4,7-di(2,3)-dihydrothienol [3,4-b][1,4]dioxin-5-yl-benzo[1,2,5]thiadiazole) (PBDT) and poly(4,7di(2,3)-dihydrothienol [3,4-b][1,4]dioxin-5-yl-2,1,3-benzoselenadiazole) (PESeE) were electrochemically deposited on graphite electrodes and used as immobilization matrices for biosensing studies. After electrochemical deposition of the polymeric matrices, glucose oxidase (GOx) was immobilized on the modified electrodes as the model enzyme. In the biosensing studies, the decrease in oxygen level as a result of enzymatic reaction was monitored at −0.7 V vs Ag/AgCl (3.0 M KCl) and correlated with substrate concentration. The biosensor was characterized in terms of several parameters such as operational and storage stabilities, kinetic parameters (K m and I max ) and surface morphologies. The biosensor was tested on real human blood serum samples.

Research paper thumbnail of Functionalization of poly-SNS-anchored carboxylic acid with Lys and PAMAM: surface modifications for biomolecule immobilization/stabilization and bio-sensing applications

The Analyst, 2012

Poly(2-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl) (SNS) acetic acid) was electrochemically deposited ... more Poly(2-(2,5-di(thiophen-2-yl)-1H-pyrrol-1-yl) (SNS) acetic acid) was electrochemically deposited on graphite electrodes and functionalized with lysine (Lys) amino acid and poly(amidoamine) derivatives (PAMAM G2 and PAMAM G4) to investigate their matrix properties for biosensor applications. Glucose oxidase (GOx) was immobilized onto the modified surface as the model enzyme. X-Ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) were used to report the surface properties of the matrices in each step of the biosensor construction. The biosensors were characterized in terms of their operational and storage stabilities and the kinetic parameters (K app m and I max ). Three new glucose biosensors revealed good stability, featuring low detection limits (19.0 mM, 3.47 mM and 2.93 mM for lysine-, PAMAM G2-and PAMAM G4-functionalized electrodes, respectively) and prolonged the shelf lives (4, 5, and 6 weeks for Lys-, PAMAM G2-and PAMAM G4-modified electrodes, respectively). The proposed biosensors were tested for glucose detection on real human blood serum samples.

Research paper thumbnail of Preparation, Characterization and H2O2 Selectivity of Hyperbranched Polyimides Containing Triazine

Journal of Polymer Research, 2005

Novel polyimides based on aromatic dianhyride and various hexahydrotriazine monomers were synthes... more Novel polyimides based on aromatic dianhyride and various hexahydrotriazine monomers were synthesized via two-stage solution polycondensation method. The resulting polyimides were characterized by solubility, viscosity, density, spectroscopic and thermal analysis ...