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Papers by Klaus-dieter Weltmann

Controlling the Effluent Chemistry of a CAP jet for Biomedical Applications: FTIR Diagnostics and Gas Phase Modeling

APS Annual Gaseous Electronics Meeting Abstracts, Sep 1, 2013

Journal of Physics D, Jan 29, 2014

HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific r... more HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L'archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d'enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Copyright

Vakuum in Forschung Und Praxis, Oct 1, 2014

Journal of Physics D, Apr 8, 2010

Although atmospheric pressure plasma jets (APPJ) have been used for several years in wide fields ... more Although atmospheric pressure plasma jets (APPJ) have been used for several years in wide fields of application, there is still lack of knowledge about physical aspects, namely the phenomenon of spatiotemporally localized luminous effects in the effluent of APPJ, so called "plasma bullets". The present paper reports on investigations on the effluent of a low frequency (kHzrange) driven APPJ in argon atmosphere. To gain insight into the spatiotemporal structure of the effluent, laser absorption measurements, probing the optical depth of the 4s 3 P 2 -4p 3 D 3 transition of argon (811.531 nm), which is proportional to the column density of the metastable 4s 3 P 2 argon atoms and investigations with an intensified video camera were performed. Simultaneous recordings of the discharge current show that plasma bullets are connected only to a certain current pulse in a series of four pulses of the discharge cycle. A good correlation between the propagation speeds of the plasma bullets (5-20 km/s) and of the generation zone of metastable argon atoms in the effluent of the APPJ (8-25 km/s) was found. Both, the appearance of plasma bullets and spatiotemporal evolution of the generation zone of metastable atoms can be explained by the effect of a self-propagating ionization front.

Plasma Sources Science and Technology, May 16, 2012

HAL (Le Centre pour la Communication Scientifique Directe), Jul 15, 2019

IEEE Transactions on Plasma Science, Sep 1, 2015

IEEE Transactions on Plasma Science, Oct 1, 2014

Biomolecules & Therapeutics, Nov 30, 2014

Non-thermal atmospheric-pressure plasma, also named cold plasma, is defined as a partly ionized g... more Non-thermal atmospheric-pressure plasma, also named cold plasma, is defined as a partly ionized gas. Therefore, it cannot be equated with plasma from blood; it is not biological in nature. Non-thermal atmospheric-pressure plasma is a new innovative approach in medicine not only for the treatment of wounds, but with a wide-range of other applications, as e.g. topical treatment of other skin diseases with microbial involvement or treatment of cancer diseases. This review emphasizes plasma effects on wound healing. Non-thermal atmospheric-pressure plasma can support wound healing by its antiseptic effects, by stimulation of proliferation and migration of wound relating skin cells, by activation or inhibition of integrin receptors on the cell surface or by its pro-angiogenic effect. We summarize the effects of plasma on eukaryotic cells, especially on keratinocytes in terms of viability, proliferation, DNA, adhesion molecules and angiogenesis together with the role of reactive oxygen species and other components of plasma. The outcome of first clinical trials regarding wound healing is pointed out.

Applied sciences, Mar 13, 2020

Cutaneous melanoma is the most aggressive type of skin cancer with a not-sufficient clinical outc... more Cutaneous melanoma is the most aggressive type of skin cancer with a not-sufficient clinical outcome. High tumor mutation rates often hamper a remedial treatment, creating the need for palliative care in many patients. To reduce pain and burden, local palliation often includes cryo-ablation, immunotherapy via injection of IL2, or electrochemotherapy. Yet, a fraction of patients and lesions do not respond to those therapies. To reach even these resistances in a redox-mediated way, we treated skin biopsies from human melanoma ex vivo with cold physical plasma (kINPen MED plasma jet). This partially ionized gas generates a potent mixture of reactive oxygen species (ROS). Physical plasmas have been shown to be potent antitumor agents in preclinical melanoma and clinical head and neck cancer research. The innovation of this technology lies in its ease-of-use without anesthesia, as the "cold" plasma temperature of the kINPen MED does not exceed 37 • C. In metastatic melanoma skin biopsies from six patients, we identified a marked increase of apoptosis with plasma treatment ex vivo. This had an impact on the chemokine/cytokine profile of the cultured biopsies, e.g., three of six patient-derived biopsy supernatants showed an apparent decrease in VEGF compared to non-plasma treated specimens. Moreover, the baseline release levels of 24 chemokines/cytokines investigated may serve as a useful tool for future research on melanoma skin biopsy treatments. Our findings suggest a clinically useful role of cold physical plasma therapy in palliation of cutaneous melanoma lesions, possibly in a combinatory setting with other immune therapies.

Journal of Physics D, Mar 27, 2014

Journal of Physics D, Mar 20, 2014

Bulletin of the American Physical Society, Oct 4, 2013

Reactive Species Processes in Plasma-, Gas-, and Liquid-Phase 1 STEPHAN REUTER, JOERN WINTER, MAL... more Reactive Species Processes in Plasma-, Gas-, and Liquid-Phase 1 STEPHAN REUTER, JOERN WINTER, MALTE HAMMER, ANSGAR SCHMIDT-BLEKER, SYLVAIN ISENI, HELENA TRESP, MARIO D ÜNNBIER, KAI MASUR, KRISTIAN WENDE, ZIK plasmatis at the INP Greifswald, KLAUS-DIETER WELTMANN, INP Greifswald -Especially for the field of plasma medicine, plasmas interacting with liquids are of great interest for environmental, chemical, and biomedical applications. In this work we present optical diagnostics on atmospheric pressure plasma jets interacting with liquids. Combining the diagnostic results with numerical simulations yields an understanding of fundamental processes such as air species diffusion into the jet effluents or the influence on humidity. Especially for plasma treatment of physiological liquids in ambient air, atmospheric species play a key role. To achieve a desired reactive component output, the generation processes from these ambient air species are controlled. Plasma jets are characterized by planar laser induced fluorescence spectroscopy, by absorption and emission spectroscopy, and by flow simulations. With the gained knowledge we are able to tailor the reactive component composition and to influence plasma jetliquid interaction. We show that reactive species generation within plasma treated liquid can be tuned and apply the findings to biological cells to investigate the effect of reactive oxygen and nitrogen species (RONS). The plasma treated liquids are investigated regarding their pH value, OH radicals, nitrate and nitrite, and H 2 O 2 content. From the tailored plasma treatment a significant insight into the relevant transport processes in plasma treatment of liquids has been gained. 1 Support by the German BMBF 03Z2DN11&12 is acknowledged.

Bulletin of the American Physical Society, Nov 15, 2011

Investigation of ambient air species diffusion into the effluent of an atmospheric pressure plasm... more Investigation of ambient air species diffusion into the effluent of an atmospheric pressure plasma jet by measurements and modeling ANS-GAR SCHMIDT-BLEKER, STEPHAN REUTER, J ÖRN WINTER, HARTMUT LANGE, KLAUS-DIETER WELTMANN, Leibniz Institute for Plasma Science and Technology (INP) Greifswald, LEIBNIZ INSTITUTE FOR PLASMA SCIENCE AND TECHNOLOGY (INP) GREIFSWALD TEAM -The diffusion of ambient air species into the effluent of a cold atmospheric pressure plasma (CAP) jet operated with pure argon is quantified using both experimental methods and theoretical estimations by a convection-diffusion approach. In the effluent of CAP jets operated in ambient air, reactive oxygen species (ROS) and reactive nitrogen species (RNS) are generated. ROS and RNS are believed to play a central role in biomedical applications of low temperature atmospheric pressure plasmas. The inflow of atmospheric oxygen is determined by a novel absorption technique in the VUV spectral range, where emission originating from within the discharge is used as light source. An analytic expression for the estimation of the on-axis density of ambient species was obtained assuming a stationary drift-diffusion equation and is compared to complete numerical results. The easy to use expression correlates well with the experimental results obtained.

Bulletin of the American Physical Society, Oct 26, 2012

Feed gas humidity introduced into a MHz atmospheric pressure argon plasma jet affects plasma-gene... more Feed gas humidity introduced into a MHz atmospheric pressure argon plasma jet affects plasma-generated species and plasma-treated human skin cells 1 J ÖRN WINTER, KRISTIAN WENDE, MALTE U. HAMMER, HELENA TRESP, SYLVAIN ISENI, MARIO D ÜNNBIER, KAI MASUR, ZIK plasmatis at the INP Greifswald e.V., KLAUS-DIETER WELTMANN, INP Greifswald, STEPHAN REUTER, ZIK plasmatis at the INP Greifswald e.V. -It is known, that gas humidity is an important parameter in plasma treatment of biological systems under ambient conditions. However, humidity in the feed gas of an atmospheric pressure plasma jet is even more crucial than ambient humidity since humid working gas is transported through the active plasma zone and the water molecules become dissociated. The so produced oxygen/hydrogen species are significant for the active plasma component composition. In this work the effect of feed gas humidity on the plasma, on plasma-treated cell growth medium (RPMI) and subsequently on human skin cells is investigated. It is shown, that already small concentrations of humidity (<1000 ppm) induce changes in the optical emission spectrum of the effluent, increase H 2 O 2 concentration in liquid cell growth medium and inhibit human skin cell proliferation. Jörn Winter ZIK plasmatis at the INP Greifswald e.V.

Bulletin of the American Physical Society, Oct 26, 2012

Influence of plasma-treatments on the structure, superstructure, and function of membrane lipids ... more Influence of plasma-treatments on the structure, superstructure, and function of membrane lipids 1 MALTE U. HAMMER, ENRICO FORBRIG, ZIK plasmatis at the INP Greifswald e.V., KLAUS-DIETER WELTMANN, INP Greifswald e.V., STEPHAN REUTER, ZIK plasmatis at the INP Greifswald e.V. -Every cell, eu-or prokaryotic, has a membrane as an interface to the environment. Every substance that is applied from outside the cell has to interact with it. This includes plasma-generated reactive species in the liquid cell environment created by plasma-treatment. By the Singer and Nicolson model, proteins are embedded in a lipid bilayer. Proteins are the functional elements, lipids are the structural elements. Due to the amphiphilic nature of the lipids, they form (super-) structures in an aqueous environment. The exact superstructure is determined by a structural parameter of the lipid, its shape. Here, we show experiments on lipids by fluorophore-based liposome assays and raman spectroscopy. The results show a membrane-activity of plasma-born reactive species against lipids and lipid structures. Based on this results and literature, we propose a model for a lesion-forming mechanism in membranes of some reactive species created by plasma-treatment. It is based on a hydrophobic-hydrophilic mismatch due to lipid peroxidization induced by reactive species generated in liquids by plasma-treatment.

Bulletin of the American Physical Society, Nov 4, 2014

Controlling the Effluent Chemistry of a CAP jet for Biomedical Applications: FTIR Diagnostics and Gas Phase Modeling

APS Annual Gaseous Electronics Meeting Abstracts, Sep 1, 2013

Journal of Physics D, Jan 29, 2014

Vakuum in Forschung Und Praxis, Oct 1, 2014

Journal of Physics D, Apr 8, 2010

Plasma Sources Science and Technology, May 16, 2012

HAL (Le Centre pour la Communication Scientifique Directe), Jul 15, 2019

IEEE Transactions on Plasma Science, Sep 1, 2015

IEEE Transactions on Plasma Science, Oct 1, 2014

Biomolecules & Therapeutics, Nov 30, 2014

Applied sciences, Mar 13, 2020

Journal of Physics D, Mar 27, 2014

Journal of Physics D, Mar 20, 2014

Bulletin of the American Physical Society, Oct 4, 2013

Bulletin of the American Physical Society, Nov 15, 2011

Bulletin of the American Physical Society, Oct 26, 2012

Bulletin of the American Physical Society, Nov 4, 2014