EDUARDO TARIFENO | Universidad de Concepcion (original) (raw)

Papers by EDUARDO TARIFENO

Data supporting the manuscript "Role of the alga <i>Chondracanthus chamissoi</i>... more Data supporting the manuscript "Role of the alga <i>Chondracanthus chamissoi</i> (C. Agardh) Kützing 1843 in the induction of primary settlement of mitilids larvae" Abstract: Mytilid aquaculture commonly depends on wild seeds captured from natural banks and their scarcity affects the commercial production of mussels. As interest in biotechnological applications of seaweeds is increasing among coastal populations, this work evaluates the use of extracts from the red macroalgae Chondracanthus chamissoi (commonly known as "chicoria de mar") as a possible inducer of mytilid larvae primary settlement. This alga was observed to have in their natural environment interaction with diverse mytilidae. Thus, algae extracts of different polarity were prepared and used in an in vivo experiment where larvae of three different mytilid species were induced to settle, being these Mytilus galloprovincialis, Semimytilus algosus and Choromytilus chorus. This is a promising method that can be used to induce mytilid larvae settlement in open sea, taking advantage of the natural ecological relationship between the red alga C. chamissoi and the diverse species of mussels present among the Chilean coast.

Revista de biología marina y oceanografía, 2010

Los ictiólogos chilenos hemos querido hacer un homenaje a nuestro amigo y colega, el Profesor Ism... more Los ictiólogos chilenos hemos querido hacer un homenaje a nuestro amigo y colega, el Profesor Ismael Kong Urbina a través de un volumen especial en la Revista de Biología Marina y Oceanografía, comunicando algunos de nuestros resultados científicos acerca de la biología de peces, nuestro punto de encuentro en las ciencias.

Fish Physiology and Biochemistry, Sep 1, 2010

We sought to provide a useful indicator of the presence of endocrine-disrupting contaminants alon... more We sought to provide a useful indicator of the presence of endocrine-disrupting contaminants along the marine coast of the South Pacific using Chilean flounder (Paralichthys adspersus). In light of the lack of information on vitellogenin for this species, we induced, purified, and identified the plasma vitellogenin of Chilean flounder inhabiting the Chilean coast. Vitellogenin (Vg) from Chilean flounder was purified by size exclusion and ionexchange chromatography using plasma from juvenile males induced by injecting 17b-estradiol. The Vg was detected by SDS-PAGE and Western blot analyses using an antibody against turbot (Scophthalmus maximus) vitellogenin. These analyses revealed a protein band of 205 kDa and three minor bands of 120, 90, and 68 kDa. These proteins were identified as Vg by means of mass spectrometry (LCQ Duo ESI-IT-MS), matching sequences of tryptic peptides to known sequences for several other fish species. The matches showed the presence of vitellogenin (VgI, VgII, Vg A and Vg B) in Chilean flounder, similar to species such as mummichog (Fundulus heteroclitus), Japanese medaka (Oryzias latipes), and white perch (Morone americana). These results are discussed in terms of identifying Vg in Paralichthys adspersus with the antibody to turbot Vg. Moreover, we compare the molecular size of Vg from Chilean flounder (large) with that of other flatfish species. Finally, we discuss the potential use of this molecule as a biomarker for the presence of xeno-estrogenic compounds along the Chilean coastline.

Fish Physiology and Biochemistry, 2005

This paper evaluates the response of trout exposed to acute hypoxia between 8.7 mgO2/l (controls)... more This paper evaluates the response of trout exposed to acute hypoxia between 8.7 mgO2/l (controls) and 1.4 mgO2/l. Temperature, pH, NH4, oxygen consumption rate and spleen-somatic index (SSI) were evaluated. Blood cell counts, oxidative radicals (ORs) production and immature erythrocytes were assessed to evaluate their relationship with the degree of hypoxia. Acute hypoxia induced a decrease in water pH (p

The Journal of Experimental Biology, Dec 1, 1970

Archives of Environmental Contamination and Toxicology, 2009

Se entregan las referencias bibliográficas sobre taxonomía, sistemática y distribución geográfica... more Se entregan las referencias bibliográficas sobre taxonomía, sistemática y distribución geográficas de los phyla Echiura. Priapula y Sipuncula disponibles en la literatura principal y secundaria para los ambientes marinos de Chile.

The effect of CO2-driven ocean acidification (OA) on marine biota has been extensively studied mo... more The effect of CO2-driven ocean acidification (OA) on marine biota has been extensively studied mostly on a single stage of the life cycle. However, the cumulative and population-level response to this global stressor may be biased due to transgenerational effects and their impacts on physiological plasticity. In this study, we exposed adult mussels Mytilus chilensis undergoing gametogenesis to two pCO2 levels (550 and 1200 μatm) for 16 weeks, aiming to understand if prolonged exposure of reproductive individuals to OA can affect the performance of their offspring, which, in turn, were reared under multiple stressors (pCO2, temperature, and dissolved cadmium). Our results indicate dependence between the level of pCO2 of the broodstock (i.e., parental effect) and the performance of larval stages in terms of growth and physiological rates, as a single effect of temperature. While main effects of pCO2 and cadmium were observed for larval growth and ingestion rates, respectively, the com...

Aquaculture Research, 2017

In Chile, the species Choromytilus chorus stands out for its ecological and economic importance. ... more In Chile, the species Choromytilus chorus stands out for its ecological and economic importance. This mussel has a very particular condition in the colour of the gonad, being cream-yellow colour the male gonad and dark brown the female which is hardly desired by the consumers. In this context, the aim of this research is to determine the role that sex steroids have in gonadal differentiation of the mussel C. chorus. For this, juveniles with sizes 15-22 mm were selected, which were subjected to acute exposure in the form of bath for 60 days to four treatments: T1: dihydrotestosterone (DHT); T2: 17b-estradiol (E2), T3: fadrozole (F) and T4: DHT-F (DF), plus a control without chemicals. Each treatment as well as control included three replicates with 90 individuals each. The mussels undergoing treatment with E2 had a sex ratio (male:female) of 0.47 compared to 1:1 in the control group. In contrast, in the groups treated with DHT, F and DF the sex ratio changed to 2.0, 1.60 and 1.70 respectively. In the fertilization trials, all the mussels produced functional gametes, as they were able to generate gametes that were developed to morula, and veliger larva. Nevertheless, it should be noted that the size of oocytes from the group exposed to estradiol (61.12 AE 2.40 lm) was significantly higher than in other groups. These results support the hypothesis that sex steroids would be involved in sexual differentiation of marine bivalves.

Anales Del Centro De Ciencias Del Mar Y Limnologia Unam, 1980

Ocean & Coastal Management, 2002

The Agenda 21, the major document outcoming from the conference at Rio 1992, includes Chapter 17,... more The Agenda 21, the major document outcoming from the conference at Rio 1992, includes Chapter 17, where the challenge for an appropriate management of the Ocean and Coastal ecosystems, was addressed. This paper attempts to analyze the Latin American experiences and perspectives to accomplish the goals for human resource development needed for the sustainable management of Oceans and Coasts according to the Agenda 21. To do so, the following topics have been developed: (i) short review on the status of the scientific research and formation in Latin America, (ii) analysis of the major trends and patterns for educational partnership in Marine Sciences and Technology in the region, (iii) examination of the areas of the Chapter 17 that have been best accomplished in the region, (iv) examples of bilateral efforts between European and Latin American institutions for regional scientific development, (v) main problems for establishing educational partnerships on Oceans and Coastal issues. Finally, some initiatives for a plan of action, are proposed.

DOAJ (DOAJ: Directory of Open Access Journals), 2012

DOAJ (DOAJ: Directory of Open Access Journals), 2005

Latin American Journal of Aquatic Research, Mar 1, 2014

Environmental Biology of Fishes, 2007

We assessed differences in the development rates among anchoveta, E. ringens populations from the... more We assessed differences in the development rates among anchoveta, E. ringens populations from the extremes of its range under different temperatures. Time to hatch decreased with increasing temperature but there was no difference in the temperature–development time relationship between eggs from different localities. These results and observations of hatching success differences between populations at lower temperatures support the hypothesis that

Frontiers in Physiology, 2018

The effect of CO 2-driven ocean acidification (OA) on marine biota has been extensively studied m... more The effect of CO 2-driven ocean acidification (OA) on marine biota has been extensively studied mostly on a single stage of the life cycle. However, the cumulative and population-level response to this global stressor may be biased due to transgenerational effects and their impacts on physiological plasticity. In this study, we exposed adult mussels Mytilus chilensis undergoing gametogenesis to two pCO 2 levels (550 and 1200 µatm) for 16 weeks, aiming to understand if prolonged exposure of reproductive individuals to OA can affect the performance of their offspring, which, in turn, were reared under multiple stressors (pCO 2 , temperature, and dissolved cadmium). Our results indicate dependence between the level of pCO 2 of the broodstock (i.e., parental effect) and the performance of larval stages in terms of growth and physiological rates, as a single effect of temperature. While main effects of pCO 2 and cadmium were observed for larval growth and ingestion rates, respectively, the combined exposure to stressors had antagonistic effects. Moreover, we found a suppression of feeding activity in post-spawning broodstock upon high pCO 2 conditions. Nevertheless, this observation was not reflected in the final weight of the broodstock and oocyte diameter. Due to the ecological and socioeconomic importance of mussels' species around the globe, the potential implications of maternal effects for the physiology, survival, and recruitment of larvae under combined global-change stressors warrant further investigation.