Archana Mehrotra | Invertis University (original) (raw)

Papers by Archana Mehrotra

International Research Journal of Modernization in Engineering Technology and Science, Nov 27, 2023

UTTAR PRADESH JOURNAL OF ZOOLOGY

The use of spectroscopic analysis, particularly UV spectrophotometer, is a simple and essential t... more The use of spectroscopic analysis, particularly UV spectrophotometer, is a simple and essential technique for bulk drug estimation, formulation studies, and compatibility assessments of drugs with various excipients. In the pharmaceutical industry, various analytical instruments, including Fourier transform infrared spectroscopy (FTIR), are employed for investigating drug-excipient interactions that can impact the stability of active pharmaceutical ingredients. This study aimed to develop a UV spectrophotometric method for the analysis of Pioglitazone hydrochloride in phosphate buffer (pH 7.4) and methanolic solution, assessing its linearity and compliance with Beer's Law. Furthermore, we aimed to use FTIR to characterize potential interactions between Pioglitazone and common pharmaceutical excipients, such as Guar Gum, Chitosan, and Sodium Alginate. Standard solutions of Pioglitazone were prepared in phosphate buffer (pH 7.4) and methanol. UV spectrophotometer was conducted to ...

Uttar Pradesh Journal of Zoology (Uttar Pradesh J. Zool, 2023

International Journal of Drug Delivery Technology (IJDDT), 2023

Most of mosquito-repellent products and devices are made up of synthetic materials presenting mar... more Most of mosquito-repellent products and devices are made up of synthetic materials presenting market which causes various harmful effects on human beings. The resistance can be developed by the mosquito due to continuous exposure at high doses. Hence, the present research work represents the development and evaluation of mosquito repellent sticks with the help of various herbal products such as starch powder, wood powder, charcoal powder, eucalyptus oil, coconut oil, lavender oil, lemongrass and cinnamon oil, peppermint and citronella, neem oil making them ozone-friendly, financial effective, non-harmful.

Chemical & Pharmaceutical Bulletin, Dec 24, 2010

The aim of this work was to prepare chitosan nanoparticles loaded with antineoplastic drug Lomust... more The aim of this work was to prepare chitosan nanoparticles loaded with antineoplastic drug Lomustine
(LCNPs), by ionic-gelation method with homogenization. The nanoparticles were characterized for particle size,
polydispersity index (PDI), surface morphology, encapsulation efficiency, in-vitro drug release and cytotoxicity on
human lung cancer cell line L132 by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.
The particle size, zeta potential and encapsulation efficiency of prepared nanoparticles ranged from 751.1 to
6371.6 nm (PDI from 0.050.001 to 0.180.007), 37.20.21 to 53.80.18 mV and 66.741.4 to 98.01.8% respectively.
The particles were spherical in shape with smooth surface in scanning electron microscopy (SEM) and
transmission electron microscopy (TEM) images. Mechanical shearing by homogenization treatment significantly
changed the nanoparticle size. The drug release rate was biphasic and diffusion controlled over the 8 h. LCNPs
greatly inhibited the growth of the L132 cancer cell line used in this study in comparison to the native Lomustine
(LMT).

Journal of nanomedicine & biotherapeutic discovery, 2015

The incorporation of lomustine, a hydrophobic anticancer drug into PLGA nanoparticles by interfac... more The incorporation of lomustine, a hydrophobic anticancer drug into PLGA nanoparticles by interfacial deposition method was optimized. Based on the optimal parameters, it was found that lomustine-PLGA nanoparticles with acceptable properties could be obtained. Optimization of formulation variables to control the size and drug entrapment efficiency of the prepared nanoparticles seems to be based on the same scientific principles as drug-loaded nanoparticles prepared by nanoprecipitation, solvent evaporation method. The process was the most important factor to control the particle size, while both the drug-polymer interaction and the partition of drug in organic and aqueous phases were the crucial factors to govern the drug entrapment efficiency. PLGA concentration at lower level (100 mg), 1:5 organic phase: aqueous ratio, 1%w/v PVA concentration, 3%w/v pluronic F68 achieved smaller particle size. Additionally, L:G ratio of PLGA 75:25, lower volume of organic solvent (1:10 organic phase: aqueous phase), higher initial drug content (10mg) enhanced the drug entrapment efficiency and maintained lomustine concentration in blood for an extended time period, elevated lomustine concentration in lungs and slowed the elimination of lomustine. The biodistribution profiles of prepared nanoparticles in albino mice showed higher plasma drug concentration for longer period of time, elevated drug concentration in lungs and slow elimination from kidney. No toxic effects of prepared nanoparticles were observed in histopathological examination of lungs and kidney. The systematic investigation reported here promises the development of PLGA nanoparticles loaded with lomustine when tested in Lung Cancer cell line L132 and toxicological/ histopathological studies in albino mice.

Journal of Nanomedicine & Nanotechnology, 2012

This work was focused on identiﬁcation and evaluation of process parameters of modiﬁed nanoprecip... more This work was focused on identiﬁcation and evaluation of process parameters of modiﬁed nanoprecipitation method,
for fabrication of lomustine nanoparticles, with the aim of reducing cancer cell viability at low concentration of lomustine.
The parameters controlling particle size, mostly in nanosize, were solvent/nonsolvent composition and emulsiﬁcation
speed of homogenizer along with aqueous phase volume. This controlled particle size is below 250 nm. The stabilizer
concentration controlled particle size is within 68 nm ± 0.89 to 137 ± 0.94 nm with PDI 0.06 ± 0.008 to 0.25 ± 0.001. But,
the stabilizer addition mode showed more uniform size distribution with PDI 0.085 ± 0.004. Entrapment efﬁciency was
maintained well above 47 ± 0.23%. The drug release pattern was monophasic with controlled release over 24 hrs. In
the method used, drug content was affected by ratio of polymer to drug to organic solvent, as well as homogenization
speed and time. Percentage viable cells of L132 human lung cancer cell line remained, were only 5% at 100 µg/ml
lomustine equivalent PLA nanoparticles.

Journal of Pharmacovigilance, Dec 1, 2014

Journal of Nanomedicine & Nanotechnology, 2010

This study was aimed to develop lomustine loaded chitosan nanoparticles using a homogenization an... more This study was aimed to develop lomustine loaded chitosan nanoparticles using a homogenization and spray drying
technique. Effect of crosslinking agents (sodium tripolyphosphate (TPP), and sodium hexametaphosphate (HMP)) were
studied on the leaching of drug, water uptake of hydrogels, drug release from matrix and its mechanism. Nanoparticles
were obtained in the average size range of 111±16.2 to 942±11.7 nm with polydispersity index (PDI) from 0.116±0.039 to
0.517±0.037. Zeta potential of nanoparticles was ranged from 29.0±1.1 to 56.0±1.1 mV. The % encapsulation efﬁciency
of nanoparticles ranged between 58±0.88% and 96±0.51%.nanoparticles were coated with PEG 6000 to modulate drug
release. Swelling index of chitosan-TPP and chitosan-TPP-PEG nanoparticles was about 428% and 350% over the
4 h and it was more (about 465% and 395%) for chitosan-HMP and chitosan-HMP-PEG nanoparticles. Drug release
was sustained and diffusion controlled. Optimized formulation was tested for anticancer activity and drug retention
study. Cytotoxicity on human lung cancer cell line L132 was studied by trypan blue dye exclusion test. Drug loaded
nanoparticles killed L132 cells more efﬁciently than the corresponding drug alone (p< 0.05). Due to the increased
surface area lomustine loaded TPP and HMP crosslinked chitosan nanoparticles showed better anticancer activity.

The present study describes the use of an aqueous solution containing a blend of hydrotropic solu... more The present study describes the use of an aqueous solution containing a blend of hydrotropic solubilizing agents (mixed hydrotropic substance’s solution) as a successful solvent system utilizing the concept of mixed hydrotropy for spectrophotometric analytical estimation of various conventional formulations as well as novel drug delivery systems. Frusemide, a poorly water-soluble drug, was estimated by application of mixed hydrotropic solubilization method. There was more than 15-fold enhancement in aqueous solubility of frusemide in a solution of blend of hydrotropic agents which consisted of 30% urea, 13.6% sodium acetate and 11.8% sodium citrate. This solvent mixture was employed to solubilize the drug from the fine powder of tablet formulations as well as the niosomes of frusemide. The selected λmax for spectrophotometric estimation was 333 nm. The hydrotropic agents used in the analysis and additives used in the manufacture of tablets and preparation of niosomes did not interfere in the analysis. Statistical data proved the accuracy, reproducibility and precision of the proposed method. The results suggested that proposed method is new, rapid, simple, accurate, and reproducible as well as employed aqueous solvent instead of organic solvents in estimation of drug from the dosage forms.

This is a Recovery Plan prepared under the Commonwealth Environment Protection and Biodiversity C... more This is a Recovery Plan prepared under the Commonwealth Environment Protection and Biodiversity Conservation Act 1999, with the assistance of funding provided by the Australian Government. This Recovery Plan has been developed with the involvement and cooperation of a range of stakeholders, but individual stakeholders have not necessarily committed to undertaking specific actions. The attainment of objectives and the provision of funds may be subject to budgetary and other constraints affecting the parties involved. Proposed actions may be subject to modification over the life of the plan due to changes in knowledge. Disclaimer This publication may be of assistance to you but the State of Victoria and its employees do not guarantee that the publication is without flaw of any kind or is wholly appropriate for your particular purposes and therefore disclaims all liability for any error, loss or other consequence that may arise from you relying on any information in this publication.

This study was aimed to develop lomustine loaded chitosan nanoparticles using a homogenization an... more This study was aimed to develop lomustine loaded chitosan nanoparticles using a homogenization and spray drying technique. Effect of crosslinking agents (sodium tripolyphosphate (TPP), and sodium hexametaphosphate (HMP)) were studied on the leaching of drug, water uptake of hydrogels, drug release from matrix and its mechanism. Nanoparticles were obtained in the average size range of 111±16.2 to 942±11.7 nm with polydispersity index (PDI) from 0.116±0.039 to 0.517±0.037. Zeta potential of nanoparticles was ranged from 29.0±1.1 to 56.0±1.1 mV. The % encapsulation effi ciency of nanoparticles ranged between 58±0.88% and 96±0.51%.nanoparticles were coated with PEG 6000 to modulate drug release. Swelling index of chitosan-TPP and chitosan-TPP-PEG nanoparticles was about 428% and 350% over the 4 h and it was more (about 465% and 395%) for chitosan-HMP and chitosan-HMP-PEG nanoparticles. Drug release was sustained and diffusion controlled. Optimized formulation was tested for anticancer activity and drug retention study. Cytotoxicity on human lung cancer cell line L132 was studied by trypan blue dye exclusion test. Drug loaded nanoparticles killed L132 cells more effi ciently than the corresponding drug alone (p< 0.05). Due to the increased surface area lomustine loaded TPP and HMP crosslinked chitosan nanoparticles showed better anticancer activity.

Journal of Nanomedicine & Biotherapeutic Discovery, 2015

The incorporation of lomustine, a hydrophobic anticancer drug into PLGA nanoparticles by interfac... more The incorporation of lomustine, a hydrophobic anticancer drug into PLGA nanoparticles by interfacial
deposition method was optimized. Based on the optimal parameters, it was found that lomustine-PLGA
nanoparticles with acceptable properties could be obtained. Optimization of formulation variables to control the
size and drug entrapment efficiency of the prepared nanoparticles seems to be based on the same scientific
principles as drug-loaded nanoparticles prepared by nanoprecipitation, solvent evaporation method. The process
was the most important factor to control the particle size, while both the drug-polymer interaction and the partition
of drug in organic and aqueous phases were the crucial factors to govern the drug entrapment efficiency. PLGA
concentration at lower level (100 mg), 1:5 organic phase: aqueous ratio, 1%w/v PVA concentration, 3%w/v pluronic
F68 achieved smaller particle size. Additionally, L:G ratio of PLGA 75:25, lower volume of organic solvent (1:10
organic phase: aqueous phase), higher initial drug content (10mg) enhanced the drug entrapment efficiency and
maintained lomustine concentration in blood for an extended time period, elevated lomustine concentration in
lungs and slowed the elimination of lomustine. The biodistribution profiles of prepared nanoparticles in albino mice
showed higher plasma drug concentration for longer period of time, elevated drug concentration in lungs and slow
elimination from kidney. No toxic effects of prepared nanoparticles were observed in histopathological examination
of lungs and kidney. The systematic investigation reported here promises the development of PLGA nanoparticles
loaded with lomustine when tested in Lung Cancer cell line L132 and toxicological/ histopathological studies in
albino mice.

Journal of Nanomedicine & Nanotechnology

This work was focused on identification and evaluation of process parameters of modified nanoprec... more This work was focused on identification and evaluation of process parameters of modified nanoprecipitation method, for fabrication of lomustine nanoparticles, with the aim of reducing cancer cell viability at low concentration of lomustine. The parameters controlling particle size, mostly in nanosize, were solvent/nonsolvent composition and emulsification speed of homogenizer along with aqueous phase volume. This controlled particle size is below 250 nm. The stabilizer concentration controlled particle size is within 68 nm ± 0.89 to 137 ± 0.94 nm with PDI 0.06 ± 0.008 to 0.25 ± 0.001. But, the stabilizer addition mode showed more uniform size distribution with PDI 0.085 ± 0.004. Entrapment efficiency was maintained well above 47 ± 0.23%. The drug release pattern was monophasic with controlled release over 24 hrs. In the method used, drug content was affected by ratio of polymer to drug to organic solvent, as well as homogenization speed and time. Percentage viable cells of L132 human lung cancer cell line remained, were only 5% at 100 µg/ml lomustine equivalent PLA nanoparticles. Journal of Nanomedicine & Nanotechnology J o u rna l of N a n o m ed icine & N a n o te chnolo g y

The aim of this work was to prepare chitosan nanoparticles loaded with antineoplastic drug Lomust... more The aim of this work was to prepare chitosan nanoparticles loaded with antineoplastic drug Lomustine (LCNPs), by ionic-gelation method with homogenization. The nanoparticles were characterized for particle size, polydispersity index (PDI), surface morphology, encapsulation efficiency, in-vitro drug release and cytotoxicity on human lung cancer cell line L132 by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The particle size, zeta potential and encapsulation efficiency of prepared nanoparticles ranged from 75؎ ؎1.1 to 637؎ ؎1.6 nm (PDI from 0.05؎ ؎0.001 to 0.18؎ ؎0.007), 37.2؎ ؎0.21 to 53.8؎ ؎0.18 mV and 66.74؎ ؎1.4 to 98.0؎ ؎1.8% respectively. The particles were spherical in shape with smooth surface in scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images. Mechanical shearing by homogenization treatment significantly changed the nanoparticle size. The drug release rate was biphasic and diffusion controlled over the 8 h. LCNPs greatly inhibited the growth of the L132 cancer cell line used in this study in comparison to the native Lomustine (LMT).

International Research Journal of Modernization in Engineering Technology and Science, Nov 27, 2023

UTTAR PRADESH JOURNAL OF ZOOLOGY

Uttar Pradesh Journal of Zoology (Uttar Pradesh J. Zool, 2023

International Journal of Drug Delivery Technology (IJDDT), 2023

Chemical & Pharmaceutical Bulletin, Dec 24, 2010

Journal of nanomedicine & biotherapeutic discovery, 2015

Journal of Nanomedicine & Nanotechnology, 2012

Journal of Pharmacovigilance, Dec 1, 2014

Journal of Nanomedicine & Nanotechnology, 2010

This study was aimed to develop lomustine loaded chitosan nanoparticles using a homogenization an... more This study was aimed to develop lomustine loaded chitosan nanoparticles using a homogenization and spray drying technique. Effect of crosslinking agents (sodium tripolyphosphate (TPP), and sodium hexametaphosphate (HMP)) were studied on the leaching of drug, water uptake of hydrogels, drug release from matrix and its mechanism. Nanoparticles were obtained in the average size range of 111±16.2 to 942±11.7 nm with polydispersity index (PDI) from 0.116±0.039 to 0.517±0.037. Zeta potential of nanoparticles was ranged from 29.0±1.1 to 56.0±1.1 mV. The % encapsulation effi ciency of nanoparticles ranged between 58±0.88% and 96±0.51%.nanoparticles were coated with PEG 6000 to modulate drug release. Swelling index of chitosan-TPP and chitosan-TPP-PEG nanoparticles was about 428% and 350% over the 4 h and it was more (about 465% and 395%) for chitosan-HMP and chitosan-HMP-PEG nanoparticles. Drug release was sustained and diffusion controlled. Optimized formulation was tested for anticancer activity and drug retention study. Cytotoxicity on human lung cancer cell line L132 was studied by trypan blue dye exclusion test. Drug loaded nanoparticles killed L132 cells more effi ciently than the corresponding drug alone (p< 0.05). Due to the increased surface area lomustine loaded TPP and HMP crosslinked chitosan nanoparticles showed better anticancer activity.

Journal of Nanomedicine & Biotherapeutic Discovery, 2015

The incorporation of lomustine, a hydrophobic anticancer drug into PLGA nanoparticles by interfac... more The incorporation of lomustine, a hydrophobic anticancer drug into PLGA nanoparticles by interfacial
deposition method was optimized. Based on the optimal parameters, it was found that lomustine-PLGA
nanoparticles with acceptable properties could be obtained. Optimization of formulation variables to control the
size and drug entrapment efficiency of the prepared nanoparticles seems to be based on the same scientific
principles as drug-loaded nanoparticles prepared by nanoprecipitation, solvent evaporation method. The process
was the most important factor to control the particle size, while both the drug-polymer interaction and the partition
of drug in organic and aqueous phases were the crucial factors to govern the drug entrapment efficiency. PLGA
concentration at lower level (100 mg), 1:5 organic phase: aqueous ratio, 1%w/v PVA concentration, 3%w/v pluronic
F68 achieved smaller particle size. Additionally, L:G ratio of PLGA 75:25, lower volume of organic solvent (1:10
organic phase: aqueous phase), higher initial drug content (10mg) enhanced the drug entrapment efficiency and
maintained lomustine concentration in blood for an extended time period, elevated lomustine concentration in
lungs and slowed the elimination of lomustine. The biodistribution profiles of prepared nanoparticles in albino mice
showed higher plasma drug concentration for longer period of time, elevated drug concentration in lungs and slow
elimination from kidney. No toxic effects of prepared nanoparticles were observed in histopathological examination
of lungs and kidney. The systematic investigation reported here promises the development of PLGA nanoparticles
loaded with lomustine when tested in Lung Cancer cell line L132 and toxicological/ histopathological studies in
albino mice.

Journal of Nanomedicine & Nanotechnology

The aim of this work was to prepare chitosan nanoparticles loaded with antineoplastic drug Lomust... more The aim of this work was to prepare chitosan nanoparticles loaded with antineoplastic drug Lomustine (LCNPs), by ionic-gelation method with homogenization. The nanoparticles were characterized for particle size, polydispersity index (PDI), surface morphology, encapsulation efficiency, in-vitro drug release and cytotoxicity on human lung cancer cell line L132 by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The particle size, zeta potential and encapsulation efficiency of prepared nanoparticles ranged from 75؎ ؎1.1 to 637؎ ؎1.6 nm (PDI from 0.05؎ ؎0.001 to 0.18؎ ؎0.007), 37.2؎ ؎0.21 to 53.8؎ ؎0.18 mV and 66.74؎ ؎1.4 to 98.0؎ ؎1.8% respectively. The particles were spherical in shape with smooth surface in scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images. Mechanical shearing by homogenization treatment significantly changed the nanoparticle size. The drug release rate was biphasic and diffusion controlled over the 8 h. LCNPs greatly inhibited the growth of the L132 cancer cell line used in this study in comparison to the native Lomustine (LMT).