Monica Beatriz Mathor | Instituto de Pesquisas Energéticas e Nucleares (IPEN-CNEN/SP) (original) (raw)

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Papers by Monica Beatriz Mathor

Research paper thumbnail of Ultrasensitive in vivo bioassay detects bioactive human growth hormone in transduced primary human keratinocytes

Journal of Endocrinological Investigation, 1998

An improved in vivo body weight gain bioassay for the potency determination of human growth hormo... more An improved in vivo body weight gain bioassay for the potency determination of human growth hormone (hGH) has been set up in "little" mice (lit/lit), a mutant derived from the C57BU6J strain. This improved assay now has a detection limit of the order of 0.05 jJg/mouse/day, which corresponds to a sensitivity about 20-fold higher than that of the most sensitive in vivo assay reported up to now: the tibia test in hypophysectomized rats or mice. This sensitivity was achieved mainly by introduction of a careful pre-assay selection and of a three injections per day schedule. The utilization of these conditions in a 2x2 factorial assay design allowed the potency determination of recombinant DNA-derived hGH (rec-hGH) in bacterial extracts with acceptable accuracy and precision, together with the greatest economy of material,

Research paper thumbnail of Hybrid gold-protein nanoparticles as radiosensitizers

Research paper thumbnail of Aperfeiçoamento de biocurativos de quitosana utilizando epicloridrina como agente reticulante

Research paper thumbnail of Estudo de fitocosmeticos - atividade do extrato de Calendula officinalis L. em celulas da derme humana

Research paper thumbnail of Gamma Ray Sterilization Study on a Perna perna Mussel Reference Material

In this study the most suitable gamma ray dose for the sterilization of a new Perna perna mussel ... more In this study the most suitable gamma ray dose for the sterilization of a new Perna perna mussel reference material was investigated. Different gamma ray doses were applied and microbiological loads of irradiated subsamples and a non-irradiated control subsample were studied. A 0.90 kGy decimal reduction value was estimated at the microbiological load test conditions and it was concluded that a 5 kGy dose is suitable for the sterilization of the mussel reference material.

Research paper thumbnail of Determinação da citotoxicidade da cafeina e cafeina com alginato siloxanetriol em adipocitos humanos obtidos de células mesenquimais de lipoaspirado

Research paper thumbnail of Avaliação da citotoxicidade direta e indireta de arcabouços de quitosana nanoestruturados com tripolifosfato de sódio visando sua utilização como biocurativos

The development of dressings made of biocompatible materials, biodegradable and bioactive, have s... more The development of dressings made of biocompatible materials, biodegradable and bioactive, have shown great potential in tissue bioengineering. Chitosan is a biomaterial with diverse biological properties that allows the development of a biodressings with satisfactory qualities. Due to the abundance, easy to obtain and antimicrobial activity, we used the crosslinked chitosan with sodium tripolyphosphate (TPP Na5P3O10), non-toxic component that offers resistance by ionic interactions between the compounds. The study aimed to evaluate a dressing with the auxiliary function in the repair and regeneration skin. Studies were performed with SEM, FT-IR and direct and indirect cytotoxicity. For the direct and indirect cytotoxicity each sample was cut and sterilized by exposure to UV lamp laminar flow for 15 minutes on each side. Then, for the indirect cytotoxicity, samples were separately placed into sterile tubes for extraction of possible toxic substances in dressings. Extracts were filte...

Research paper thumbnail of Anti-SARS-CoV-2 equine F (Ab′)2 immunoglobulin as a possible therapy for COVID-19

Scientific Reports

The new outbreak of coronavirus disease 2019 (COVID-19) has infected and caused the death of mill... more The new outbreak of coronavirus disease 2019 (COVID-19) has infected and caused the death of millions of people worldwide. Intensive efforts are underway around the world to establish effective treatments. Immunoglobulin from immunized animals or plasma from convalescent patients might constitute a specific treatment to guarantee the neutralization of the virus in the early stages of infection, especially in patients with risk factors and a high probability of progressing to severe disease. Worldwide, a few clinical trials using anti-SARS-CoV-2 immunoglobulins from horses immunized with the entire spike protein or fragments of it in the treatment of patients with COVID-19 are underway. Here, we describe the development of an anti-SARS-CoV-2 equine F(ab′)2 immunoglobulin using a newly developed SARS-CoV-2 viral antigen that was purified and inactivated by radiation. Cell-based and preclinical assays showed that the F(ab′)2 immunoglobulin successfully neutralizes the virus, is safe in...

Research paper thumbnail of Free plasma testosterone levels during the normal menstrual cycle

Journal of Endocrinological Investigation, 1985

This report describes the free or unbound testosterone levels in ten normal females during the me... more This report describes the free or unbound testosterone levels in ten normal females during the menstrual cycle, using a simplified technique of equilibrium dialysis of undiluted plasma. Total testosterone concentration fell progressively during the menstrual cycle, whereas the percent free testosterone increased from the follicular to the luteal phase. Free testosterone levels also fell but only significantly at the late luteal phase. For comparison two patients with anovulatory cycles were evaluated. Progesterone displacement of endogenous testosterone from its binding protein(s) was suggested by in vitro studies.

Research paper thumbnail of RESEARCH ARTICLE Open Access

MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a deregulat... more MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a deregulated in HNSCC, we performed gain-of-function experiments, in an attempt to outline their possible role. Our results suggest that both molecules interfere in cell proliferation through distinct processes, possibly targeting a small Severino et al. BMC Cancer 2013, 13:533

Research paper thumbnail of Development of gelatin based scaffold by gamma radiation for application as platelet rich plasma support for wound treatment

Research paper thumbnail of Free testosterone levels during the menstrual cycle in obese versus normal women

Fertility and Sterility, 1989

Research paper thumbnail of Validation of Primary Packaging for Cryopreserved Musculoskeletal Tissues

Current Frontiers in Cryopreservation, 2012

The room is also equipped with an air conditioning system, its own energy generator, and carbon g... more The room is also equipped with an air conditioning system, its own energy generator, and carbon gas backup as protection against defrosting, as well as a rigorous temperature monitoring system that generates 24-hour printed temperature recordings, and a satellite alarm system, which ensures adequate maintenance of the temperature and early detection of any flaws. Depending on the results of the analyses, the tissues are transferred to the room designated for materials liberated for use. The maximum cryopreservation period is 5 years for bone tissue and 2 years for soft or tendinous tissues.

Research paper thumbnail of Sterilized human skin graft with a dose of 25 kGy provides a privileged immune and collagen microenvironment in the adhesion of Nude mice wounds

PLoS ONE, 2022

This study aimed to report the effects of different doses of ionizing radiation on inflammatory a... more This study aimed to report the effects of different doses of ionizing radiation on inflammatory and repair stage of human skin graft adherence in Nude mice wounds. Animals were divided into transplanted with irradiated human skin grafts (IHSG) at 25 and 50 kGy (IHSG 25 kGy; IHSG 50 kGy) and non-IHSG and euthanized on the 3rd, 7th and 21st days after the surgery, by gross and microscopic changes, immunostaining for human type I collagen (Col I) and mouse Col I and Col III and inflammatory cells. We found an effectiveness of human split-thickness graft adherence in mice transplanted with IHSG 25 kGy, as well decrease in dermo-epidermal necrosis and neutrophils, lower loss of skin thickness, epithelization and neo-vascularization. Day 21 post-transplantation with IHSG 25 kGy was observed a well-preserved human skin in the border of the graft, a prominent granulation tissue in an organization by proliferated fibroblasts, Col III deposition and increased B-cells and macrophages. A comple...

Research paper thumbnail of Effects of Ionizing Radiation on Proteins in Demineralized Lyophylized or Frozen Human Bone

Research paper thumbnail of The keratinocytes were cultured and selected with 8 μg/ml BS as described in Figure 5

<b>Copyright information:</b>Taken from "Over expression of the selectable marke... more <b>Copyright information:</b>Taken from "Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells"BMC Biotechnology 2004;4():29-29.Published online 2 Dec 2004PMCID:PMC539255.Copyright © 2004 Bento et al; licensee BioMed Central Ltd. When the keratinocytes reached 70 % confluence, the cells were divided into two parts and transferred to 2 wells of a 12-well plate that contained the irradiated NIH3T3 cells. In one well the cells were maintained without BS (A) and in another with 8 μg/ml BS (B). The cells were stained with Rhodamine B after 10 days.

Research paper thumbnail of On day 1, BALB/MK cells were seeded on a 6 well plate at 4 × 10cells/well, and 1 × 10cells of the virus producing cell lines PA317/LBmSN and PA317/LXSN were seeded in 25 cmflasks

<b>Copyright information:</b>Taken from "Over expression of the selectable marke... more <b>Copyright information:</b>Taken from "Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells"BMC Biotechnology 2004;4():29-29.Published online 2 Dec 2004PMCID:PMC539255.Copyright © 2004 Bento et al; licensee BioMed Central Ltd. On day 2, the media of the PA317 cell clones were replaced with the EMEM supplemented with 10 % FBS and without EGF. On day 3, the media of PA317 cells containing virus were harvested, centrifuged at 10,000 rpm per 1 min in Eppendorf centrifuge. The media of BALB/MK cells were replaced by 2 ml of virus solution and complemented with 5 ng/ml of EGF and 8 μg/ml of Polybrene. In parallel, 1 × 10BALB/MK cells were seeded on a 24 well plate. On day 4, the media were replaced by fresh ones. On day 5, the medium of BALB/MK cells was replaced by 0.5 ml of the medium of the LBmSN transduced BALB/MK cells, which was previously centrifuged at 10,000 rpm/ 1 min. After 7 days, the cells were photographed (X 200). The results are representative of at least three experiments, which gave essentially the same results. (A) The BALB/MK cells incubated with supernatant of BALB/MK cells transduced with LXSN; (B) The BALB/MK cells incubated with supernatant of BALB/MK cells transduced with LBmSN.

Research paper thumbnail of On day 1, BALB/MK cells were seeded on a 24-well plate at 1 × 10cells/well, and 1 × 10cells of the virus producing cell lines PA317/LBmSN (2 × 10cfu/ml) and PA317/LXSN (5 × 10cfu/ml) were seeded in 25 cmflasks

<b>Copyright information:</b>Taken from "Over expression of the selectable marke... more <b>Copyright information:</b>Taken from "Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells"BMC Biotechnology 2004;4():29-29.Published online 2 Dec 2004PMCID:PMC539255.Copyright © 2004 Bento et al; licensee BioMed Central Ltd. On day 2, the media of PA317 cell clones were replaced with EMEM without EGF. On day 3, the media of PA317 cells containing virus were harvested, centrifuged at 10000 rpm per 1 min in Eppendorf centrifuge. The media of BALB/MK cells were replaced with 0.5 ml of virus solution and supplemented with 5 ng/ml EGF and 8 μg/ml Polybrene. On day 4, the media were replaced with fresh EMEM in the following conditions. After 12 days, the cells were stained with Coomassie Blue and photographed. The results are representative of at least three experiments, which gave essentially the same results. (A) BALB/MK; (B) BALB/MK + 500 μg/ml G418; (C) BALB/MK + 8 μg/ml BS; (D) BALB/MK/LXSN + 500 μg/ml G418; (E) BALB/MK/LXSN; (F) BALB/MK/LXSN + 8 μg/ml BS; (G) BALB/MK/LBmSN; (H) BALB/MK/LBmSN + 500 μg/ml G418; (I) BALB/MK/LBmSN + 0.05 μg/ml BS; (J) BALB/MK/LBmSN + 0.1 μg/ml BS; (K) BALB/MK/LBmSN + 0.2 μg/ml BS; (L) BALB/MK/LBmSN + 0.5 μg/ml BS; (M) BALB/MK/LBmSN + 1 μg/ml BS; (N) BALB/MK/LBmSN + 2 μg/ml BS; (O) BALB/MK/LBmSN + 4 μg/ml BS; (P) BALB/MK/LBmSN + 8 μg/ml BS; (Q) BALB/MK/LBmSN + 16 μg/ml BS; (R) BALB/MK/LBmSN + 32 μg/ml BS(S) Five days after the LBmSN transduction, the cells were detached with trypsin and stained with Trypan blue. Left and right sides represent BALB/MK (control) and BALB/MK/LBmSN cells respectively.

Research paper thumbnail of The cells were cultured and transduced as described in Figure 1, however the virus concentration in each plate varied as follows

<b>Copyright information:</b>Taken from "Over expression of the selectable marke... more <b>Copyright information:</b>Taken from "Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells"BMC Biotechnology 2004;4():29-29.Published online 2 Dec 2004PMCID:PMC539255.Copyright © 2004 Bento et al; licensee BioMed Central Ltd. After 10 days of the transduction, the cells were stained with Coomassie Blue and photographed. The results are representative of at least three experiments, which gave essentially the same results. (A) BALB/MK + 8 μg/ml BS; (B) BALB/MK + 10cfu of LBmSN + 8 μg/ml BS; (C) BALB/MK + 10cfu of LBmSN + 8 μg/ml BS; (D) BALB/MK + 10cfu of LBmSN + 8 μg/ml BS; (E) BALB/MK + 10cfu of LBmSN + 8 μg/ml BS; (F) BALB/MK; (G) BALB/MK + 10cfu of LBmSN; (H) BALB/MK + 10cfu of LBmSN; (I) BALB/MK + 10cfu of LBmSN; (J) BALB/MK + 10cfu of LBmSN

Research paper thumbnail of Análisis Por Espectroscopia Raman De Los Efectos De La Radiación Ionizante en El Tejido Óseo Liofilizado O Congelado

Diversos bancos de tejidos consideran esencial que los tejidos biologicos sean esterilizados por ... more Diversos bancos de tejidos consideran esencial que los tejidos biologicos sean esterilizados por un metodo eficaz, utilizando radiacion ionizante. Sin embargo, altas dosis de radiacion en los tejidos y el modo de almacenamiento pueden inducir cambios estructurales en el mismo, tales como la degradacion del hueso. Por lo tanto, es importante estudiar las alteraciones causadas en el tejido oseo luego de su preservacion, bien como su esterilizacion por varias dosis a fin de determinar la dosis optima, proporcionando esterilidad y seguridad en la estructura del material que esta siendo utilizado para el trasplante. El objetivo de este estudio fue investigar las alteraciones por espectroscopia Raman en tejido oseo humano congelado y tejido oseo humano liofilizado, sometiendolos a diferentes dosis de irradiacion (15, 25 y 50 kGy) en comparacion con el control (no irradiado). Se utilizo un espectrometro Raman Horiba, modelo Xplora ™ y los espectros Raman fueron obtenidos con excitacion a 7...

Research paper thumbnail of Ultrasensitive in vivo bioassay detects bioactive human growth hormone in transduced primary human keratinocytes

Journal of Endocrinological Investigation, 1998

An improved in vivo body weight gain bioassay for the potency determination of human growth hormo... more An improved in vivo body weight gain bioassay for the potency determination of human growth hormone (hGH) has been set up in "little" mice (lit/lit), a mutant derived from the C57BU6J strain. This improved assay now has a detection limit of the order of 0.05 jJg/mouse/day, which corresponds to a sensitivity about 20-fold higher than that of the most sensitive in vivo assay reported up to now: the tibia test in hypophysectomized rats or mice. This sensitivity was achieved mainly by introduction of a careful pre-assay selection and of a three injections per day schedule. The utilization of these conditions in a 2x2 factorial assay design allowed the potency determination of recombinant DNA-derived hGH (rec-hGH) in bacterial extracts with acceptable accuracy and precision, together with the greatest economy of material,

Research paper thumbnail of Hybrid gold-protein nanoparticles as radiosensitizers

Research paper thumbnail of Aperfeiçoamento de biocurativos de quitosana utilizando epicloridrina como agente reticulante

Research paper thumbnail of Estudo de fitocosmeticos - atividade do extrato de Calendula officinalis L. em celulas da derme humana

Research paper thumbnail of Gamma Ray Sterilization Study on a Perna perna Mussel Reference Material

In this study the most suitable gamma ray dose for the sterilization of a new Perna perna mussel ... more In this study the most suitable gamma ray dose for the sterilization of a new Perna perna mussel reference material was investigated. Different gamma ray doses were applied and microbiological loads of irradiated subsamples and a non-irradiated control subsample were studied. A 0.90 kGy decimal reduction value was estimated at the microbiological load test conditions and it was concluded that a 5 kGy dose is suitable for the sterilization of the mussel reference material.

Research paper thumbnail of Determinação da citotoxicidade da cafeina e cafeina com alginato siloxanetriol em adipocitos humanos obtidos de células mesenquimais de lipoaspirado

Research paper thumbnail of Avaliação da citotoxicidade direta e indireta de arcabouços de quitosana nanoestruturados com tripolifosfato de sódio visando sua utilização como biocurativos

The development of dressings made of biocompatible materials, biodegradable and bioactive, have s... more The development of dressings made of biocompatible materials, biodegradable and bioactive, have shown great potential in tissue bioengineering. Chitosan is a biomaterial with diverse biological properties that allows the development of a biodressings with satisfactory qualities. Due to the abundance, easy to obtain and antimicrobial activity, we used the crosslinked chitosan with sodium tripolyphosphate (TPP Na5P3O10), non-toxic component that offers resistance by ionic interactions between the compounds. The study aimed to evaluate a dressing with the auxiliary function in the repair and regeneration skin. Studies were performed with SEM, FT-IR and direct and indirect cytotoxicity. For the direct and indirect cytotoxicity each sample was cut and sterilized by exposure to UV lamp laminar flow for 15 minutes on each side. Then, for the indirect cytotoxicity, samples were separately placed into sterile tubes for extraction of possible toxic substances in dressings. Extracts were filte...

Research paper thumbnail of Anti-SARS-CoV-2 equine F (Ab′)2 immunoglobulin as a possible therapy for COVID-19

Scientific Reports

The new outbreak of coronavirus disease 2019 (COVID-19) has infected and caused the death of mill... more The new outbreak of coronavirus disease 2019 (COVID-19) has infected and caused the death of millions of people worldwide. Intensive efforts are underway around the world to establish effective treatments. Immunoglobulin from immunized animals or plasma from convalescent patients might constitute a specific treatment to guarantee the neutralization of the virus in the early stages of infection, especially in patients with risk factors and a high probability of progressing to severe disease. Worldwide, a few clinical trials using anti-SARS-CoV-2 immunoglobulins from horses immunized with the entire spike protein or fragments of it in the treatment of patients with COVID-19 are underway. Here, we describe the development of an anti-SARS-CoV-2 equine F(ab′)2 immunoglobulin using a newly developed SARS-CoV-2 viral antigen that was purified and inactivated by radiation. Cell-based and preclinical assays showed that the F(ab′)2 immunoglobulin successfully neutralizes the virus, is safe in...

Research paper thumbnail of Free plasma testosterone levels during the normal menstrual cycle

Journal of Endocrinological Investigation, 1985

This report describes the free or unbound testosterone levels in ten normal females during the me... more This report describes the free or unbound testosterone levels in ten normal females during the menstrual cycle, using a simplified technique of equilibrium dialysis of undiluted plasma. Total testosterone concentration fell progressively during the menstrual cycle, whereas the percent free testosterone increased from the follicular to the luteal phase. Free testosterone levels also fell but only significantly at the late luteal phase. For comparison two patients with anovulatory cycles were evaluated. Progesterone displacement of endogenous testosterone from its binding protein(s) was suggested by in vitro studies.

Research paper thumbnail of RESEARCH ARTICLE Open Access

MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a deregulat... more MicroRNA expression profile in head and neck cancer: HOX-cluster embedded microRNA-196a deregulated in HNSCC, we performed gain-of-function experiments, in an attempt to outline their possible role. Our results suggest that both molecules interfere in cell proliferation through distinct processes, possibly targeting a small Severino et al. BMC Cancer 2013, 13:533

Research paper thumbnail of Development of gelatin based scaffold by gamma radiation for application as platelet rich plasma support for wound treatment

Research paper thumbnail of Free testosterone levels during the menstrual cycle in obese versus normal women

Fertility and Sterility, 1989

Research paper thumbnail of Validation of Primary Packaging for Cryopreserved Musculoskeletal Tissues

Current Frontiers in Cryopreservation, 2012

The room is also equipped with an air conditioning system, its own energy generator, and carbon g... more The room is also equipped with an air conditioning system, its own energy generator, and carbon gas backup as protection against defrosting, as well as a rigorous temperature monitoring system that generates 24-hour printed temperature recordings, and a satellite alarm system, which ensures adequate maintenance of the temperature and early detection of any flaws. Depending on the results of the analyses, the tissues are transferred to the room designated for materials liberated for use. The maximum cryopreservation period is 5 years for bone tissue and 2 years for soft or tendinous tissues.

Research paper thumbnail of Sterilized human skin graft with a dose of 25 kGy provides a privileged immune and collagen microenvironment in the adhesion of Nude mice wounds

PLoS ONE, 2022

This study aimed to report the effects of different doses of ionizing radiation on inflammatory a... more This study aimed to report the effects of different doses of ionizing radiation on inflammatory and repair stage of human skin graft adherence in Nude mice wounds. Animals were divided into transplanted with irradiated human skin grafts (IHSG) at 25 and 50 kGy (IHSG 25 kGy; IHSG 50 kGy) and non-IHSG and euthanized on the 3rd, 7th and 21st days after the surgery, by gross and microscopic changes, immunostaining for human type I collagen (Col I) and mouse Col I and Col III and inflammatory cells. We found an effectiveness of human split-thickness graft adherence in mice transplanted with IHSG 25 kGy, as well decrease in dermo-epidermal necrosis and neutrophils, lower loss of skin thickness, epithelization and neo-vascularization. Day 21 post-transplantation with IHSG 25 kGy was observed a well-preserved human skin in the border of the graft, a prominent granulation tissue in an organization by proliferated fibroblasts, Col III deposition and increased B-cells and macrophages. A comple...

Research paper thumbnail of Effects of Ionizing Radiation on Proteins in Demineralized Lyophylized or Frozen Human Bone

Research paper thumbnail of The keratinocytes were cultured and selected with 8 μg/ml BS as described in Figure 5

<b>Copyright information:</b>Taken from "Over expression of the selectable marke... more <b>Copyright information:</b>Taken from "Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells"BMC Biotechnology 2004;4():29-29.Published online 2 Dec 2004PMCID:PMC539255.Copyright © 2004 Bento et al; licensee BioMed Central Ltd. When the keratinocytes reached 70 % confluence, the cells were divided into two parts and transferred to 2 wells of a 12-well plate that contained the irradiated NIH3T3 cells. In one well the cells were maintained without BS (A) and in another with 8 μg/ml BS (B). The cells were stained with Rhodamine B after 10 days.

Research paper thumbnail of On day 1, BALB/MK cells were seeded on a 6 well plate at 4 × 10cells/well, and 1 × 10cells of the virus producing cell lines PA317/LBmSN and PA317/LXSN were seeded in 25 cmflasks

<b>Copyright information:</b>Taken from "Over expression of the selectable marke... more <b>Copyright information:</b>Taken from "Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells"BMC Biotechnology 2004;4():29-29.Published online 2 Dec 2004PMCID:PMC539255.Copyright © 2004 Bento et al; licensee BioMed Central Ltd. On day 2, the media of the PA317 cell clones were replaced with the EMEM supplemented with 10 % FBS and without EGF. On day 3, the media of PA317 cells containing virus were harvested, centrifuged at 10,000 rpm per 1 min in Eppendorf centrifuge. The media of BALB/MK cells were replaced by 2 ml of virus solution and complemented with 5 ng/ml of EGF and 8 μg/ml of Polybrene. In parallel, 1 × 10BALB/MK cells were seeded on a 24 well plate. On day 4, the media were replaced by fresh ones. On day 5, the medium of BALB/MK cells was replaced by 0.5 ml of the medium of the LBmSN transduced BALB/MK cells, which was previously centrifuged at 10,000 rpm/ 1 min. After 7 days, the cells were photographed (X 200). The results are representative of at least three experiments, which gave essentially the same results. (A) The BALB/MK cells incubated with supernatant of BALB/MK cells transduced with LXSN; (B) The BALB/MK cells incubated with supernatant of BALB/MK cells transduced with LBmSN.

Research paper thumbnail of On day 1, BALB/MK cells were seeded on a 24-well plate at 1 × 10cells/well, and 1 × 10cells of the virus producing cell lines PA317/LBmSN (2 × 10cfu/ml) and PA317/LXSN (5 × 10cfu/ml) were seeded in 25 cmflasks

<b>Copyright information:</b>Taken from "Over expression of the selectable marke... more <b>Copyright information:</b>Taken from "Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells"BMC Biotechnology 2004;4():29-29.Published online 2 Dec 2004PMCID:PMC539255.Copyright © 2004 Bento et al; licensee BioMed Central Ltd. On day 2, the media of PA317 cell clones were replaced with EMEM without EGF. On day 3, the media of PA317 cells containing virus were harvested, centrifuged at 10000 rpm per 1 min in Eppendorf centrifuge. The media of BALB/MK cells were replaced with 0.5 ml of virus solution and supplemented with 5 ng/ml EGF and 8 μg/ml Polybrene. On day 4, the media were replaced with fresh EMEM in the following conditions. After 12 days, the cells were stained with Coomassie Blue and photographed. The results are representative of at least three experiments, which gave essentially the same results. (A) BALB/MK; (B) BALB/MK + 500 μg/ml G418; (C) BALB/MK + 8 μg/ml BS; (D) BALB/MK/LXSN + 500 μg/ml G418; (E) BALB/MK/LXSN; (F) BALB/MK/LXSN + 8 μg/ml BS; (G) BALB/MK/LBmSN; (H) BALB/MK/LBmSN + 500 μg/ml G418; (I) BALB/MK/LBmSN + 0.05 μg/ml BS; (J) BALB/MK/LBmSN + 0.1 μg/ml BS; (K) BALB/MK/LBmSN + 0.2 μg/ml BS; (L) BALB/MK/LBmSN + 0.5 μg/ml BS; (M) BALB/MK/LBmSN + 1 μg/ml BS; (N) BALB/MK/LBmSN + 2 μg/ml BS; (O) BALB/MK/LBmSN + 4 μg/ml BS; (P) BALB/MK/LBmSN + 8 μg/ml BS; (Q) BALB/MK/LBmSN + 16 μg/ml BS; (R) BALB/MK/LBmSN + 32 μg/ml BS(S) Five days after the LBmSN transduction, the cells were detached with trypsin and stained with Trypan blue. Left and right sides represent BALB/MK (control) and BALB/MK/LBmSN cells respectively.

Research paper thumbnail of The cells were cultured and transduced as described in Figure 1, however the virus concentration in each plate varied as follows

<b>Copyright information:</b>Taken from "Over expression of the selectable marke... more <b>Copyright information:</b>Taken from "Over expression of the selectable marker blasticidin S deaminase gene is toxic to human keratinocytes and murine BALB/MK cells"BMC Biotechnology 2004;4():29-29.Published online 2 Dec 2004PMCID:PMC539255.Copyright © 2004 Bento et al; licensee BioMed Central Ltd. After 10 days of the transduction, the cells were stained with Coomassie Blue and photographed. The results are representative of at least three experiments, which gave essentially the same results. (A) BALB/MK + 8 μg/ml BS; (B) BALB/MK + 10cfu of LBmSN + 8 μg/ml BS; (C) BALB/MK + 10cfu of LBmSN + 8 μg/ml BS; (D) BALB/MK + 10cfu of LBmSN + 8 μg/ml BS; (E) BALB/MK + 10cfu of LBmSN + 8 μg/ml BS; (F) BALB/MK; (G) BALB/MK + 10cfu of LBmSN; (H) BALB/MK + 10cfu of LBmSN; (I) BALB/MK + 10cfu of LBmSN; (J) BALB/MK + 10cfu of LBmSN

Research paper thumbnail of Análisis Por Espectroscopia Raman De Los Efectos De La Radiación Ionizante en El Tejido Óseo Liofilizado O Congelado

Diversos bancos de tejidos consideran esencial que los tejidos biologicos sean esterilizados por ... more Diversos bancos de tejidos consideran esencial que los tejidos biologicos sean esterilizados por un metodo eficaz, utilizando radiacion ionizante. Sin embargo, altas dosis de radiacion en los tejidos y el modo de almacenamiento pueden inducir cambios estructurales en el mismo, tales como la degradacion del hueso. Por lo tanto, es importante estudiar las alteraciones causadas en el tejido oseo luego de su preservacion, bien como su esterilizacion por varias dosis a fin de determinar la dosis optima, proporcionando esterilidad y seguridad en la estructura del material que esta siendo utilizado para el trasplante. El objetivo de este estudio fue investigar las alteraciones por espectroscopia Raman en tejido oseo humano congelado y tejido oseo humano liofilizado, sometiendolos a diferentes dosis de irradiacion (15, 25 y 50 kGy) en comparacion con el control (no irradiado). Se utilizo un espectrometro Raman Horiba, modelo Xplora ™ y los espectros Raman fueron obtenidos con excitacion a 7...