Tammineni Rajitha | Institute of Rural Management Anand (original) (raw)
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Papers by Tammineni Rajitha
COVID-19 Pandemic, Geospatial Information, and Community Resilience, 2021
DNA barcoding is a molecular technique that uses variations in DNA sequences to identify biologic... more DNA barcoding is a molecular technique that uses variations in DNA sequences to identify biological specimens. Unlike animals, in plants DNA barcoding is not simple. The plastid loci like rbcL, matK and trnH, are used for identification purposes but sequences of these loci are unable to provide 100% species identification across the plant kingdom. Also, difficulty in amplification and sequencing restricts their suitability as universal barcodes. Therefore, search for additional and universal barcodes for plant kingdom is imperative to further facilitate identification across taxa. Keeping this in view, the objective of the present investigation was to assess chloroplast genomes for DNA barcodes that can supplement and/or complement the existing barcodes. Based on preliminary in silico sequence analysis of chloroplast genomes of angiosperms, trnD-trnT non-coding spacer region (DT) of chloroplast genome was chosen to experimentally validate its suitability as DNA barcode. For the pres...
COVID-19 Pandemic, Geospatial Information, and Community Resilience, 2021
DNA barcoding is a molecular technique that uses variations in DNA sequences to identify biologic... more DNA barcoding is a molecular technique that uses variations in DNA sequences to identify biological specimens. Unlike animals, in plants DNA barcoding is not simple. The plastid loci like rbcL, matK and trnH, are used for identification purposes but sequences of these loci are unable to provide 100% species identification across the plant kingdom. Also, difficulty in amplification and sequencing restricts their suitability as universal barcodes. Therefore, search for additional and universal barcodes for plant kingdom is imperative to further facilitate identification across taxa. Keeping this in view, the objective of the present investigation was to assess chloroplast genomes for DNA barcodes that can supplement and/or complement the existing barcodes. Based on preliminary in silico sequence analysis of chloroplast genomes of angiosperms, trnD-trnT non-coding spacer region (DT) of chloroplast genome was chosen to experimentally validate its suitability as DNA barcode. For the pres...