Silverio García-lara | Tecnológico de Monterrey (original) (raw)

Papers by Silverio García-lara

Additional file 4: Figure S4. MS/MS identification of glycosylated Sec-G-CSF:cMyc-derived peptide... more Additional file 4: Figure S4. MS/MS identification of glycosylated Sec-G-CSF:cMyc-derived peptide. A Schematic illustration of released peptide after Trypsin/Chymotrypsin in gel digestion of c-Myc purified Sec-G-CSF:cMyc expressed alone or co-expressed with the O-glycosylation machinery. Native glycosylation site (Thr-163) is denoted by underlining. B Extracted ion chromatograms of predicted QQMEELGMAPALQPTQGAMPAFASAF peptide derived from Sec-G-CSF:cMyc expressed alone (upper panel), not being detected; or co-expressed with the O-glycosylation machinery (lower panel), where it was detected. C MS/MS of c-Myc purified and Trypsin/Chymotrypsin in gel digested Sec-G-CSF:cMyc-derived peptide (QQMEELGMAPALQPTQGAMPAFASAF) co-expressed with the O-glycosylation machinery, showing identified b- and y-ions. Modified Thr-163 is marked in red. Complete list of detected product ions is shown in Supplementary Table S1

Additional file 2: Figure S2. Secretory and Cytoplasmic G-CSF can be extracted with a PBS-based e... more Additional file 2: Figure S2. Secretory and Cytoplasmic G-CSF can be extracted with a PBS-based extraction buffer, but Zera-G-CSF can only be extracted under denaturing and reducing conditions. Western Blot detection of G-CSF:eYFP variants transiently expressed in N. benthamiana plants. Samples collected at 4 dpi. Upper panel: Plant 1; Lower panel: Plant 2. Sec-G-CSF:eYFP (Secretory variant, 49 kDa), Cyt-G-CSF:eYFP (Cytoplasmic and mature protein variant, 49 kDa), Zera-G-CSF:eYFP (Zera fused variant, 61 kDa), eYFP (Cytoplasmic eYFP, 29 kDa), p19 negative control. Four leaf discs from two different plants were collected for each sample. 50 µg TSP of PBST samples or equivalent volume of SDS-DTT samples were loaded on the gel. Black arrows denote Zera-G-CSF:eYFP. Gray arrows denote Sec-GCSF-:eYFP and Cyt-G-CSF:eYFP. Red asterisks denotes a faint band corresponding to Zera-G-CSF:eYFP extracted with PBST extraction buffer and its corresponding proper extraction with SDS-DTT extraction bu...

Background Foams are high porosity and low density materials. In nature, they are a common archit... more Background Foams are high porosity and low density materials. In nature, they are a common architec-ture. Some of their relevant technological applications include heat and sound insulation, lightweight materials, and tissue engineering scaffolds. Foams derived from natural poly-mers are particularly attractive for tissue culture due to their biodegradability and bio-com-patibility. Here, the foaming potential of an extensive list of materials was assayed, including slabs elaborated from whole flour, the starch component only, or the protein frac-tion only of maize seeds. Methodology/Principal Findings We used supercritical CO2 to produce foams from thermoplasticized maize derived materials. Polyethylene-glycol, sorbitol/glycerol, or urea/formamide were used as plasticizers. We re-port expansion ratios, porosities, average pore sizes, pore morphologies, and pore size distri-butions for these materials. High porosity foams were obtained from zein thermoplasticized with polyethylene g...

Plants

Maize (Zea mays L.) represents the main caloric source for much of the world’s population. Pigmen... more Maize (Zea mays L.) represents the main caloric source for much of the world’s population. Pigmented maize varieties are an excellent source of nutraceutical compounds: blue and yellow maize are rich in anthocyanins as well as carotenoids and phenolic acids, respectively. However, blue maize is usually grown in small quantities as a specialty crop because it lacks the qualities and adaptations of commercial white and yellow varieties. Here, a new high-yield variety of blue maize called Vitamaiz was developed from inbred lines of subtropical blue, white, and yellow maize. The aim of this study was to characterize the nutraceutical and physical properties of 30 Vitamaiz hybrids in two subtropical locations. Kernel physical traits, nutrient composition, and nutraceutical components (free phenolic acids, FPA; cell wall-bound phenolic acids, BPA; total monomeric anthocyanin content, TAC; antioxidant capacity, AOX by oxygen radical absorbance capacity assay, and total carotenoid content, ...

Social Science Research Network, 2022

Frontiers in Nutrition

Maize is one of the most heterogenous cereals worldwide in terms of yield, physical characteristi... more Maize is one of the most heterogenous cereals worldwide in terms of yield, physical characteristics, and biochemical composition due to its natural diversity. Nowadays the use of maize hybrids is extensive, while the use of landraces is mostly local. Both have become an important genetic resource useful to identify or generate varieties with desirable characteristics to overcome challenges of agronomic performance, nutritional quality, and functionality. In terms of functionality, one of the most studied families of compounds are phenolic acids. These compounds have been associated with the improvement of human health because of their antioxidant capacity. To evaluate the diversity of phenolic compounds in maize, two collections, the Nested Association Mapping (NAM) founders and 24 landraces, were crossed with B73. Phenolic compounds were extracted and quantified by HPLC-PDA. Soluble and cell wall phenolic acids were identified and significant differences between and within the NAM ...

MS/MS spectra of proteins from insect-resistant endosperms, which were purified using ion-exchang... more MS/MS spectra of proteins from insect-resistant endosperms, which were purified using ion-exchange chromatogragy . The data set contains the raw data, the *.mzML and the *.mgf files. The acquisition of the data is described in: L. Margarita López-Castillo, Alán González-Leyzaola, M. Fernanda DíazFlores-Rivera, Robert Winkler, Natalie Wielsch and Silverio García-Lara. 2020. "Modulation of Aleurone Peroxidases in Kernels of Insect-Resistant Maize (Zea mays L.; Pob84-C3R) After Mechanical and Insect Damage". 2020. Frontiers in Plant Science. Publication pending.

Additional file 1: Figure S3. Impact of extraction buffer on G-CSF. A Western Blot detection of S... more Additional file 1: Figure S3. Impact of extraction buffer on G-CSF. A Western Blot detection of Sec-G-CSF:eYFP and Cyt-G-CSF:eYFP extracted with PBST0.1 % (gels on the left) or under reducing and denaturing extraction conditions (SDS-DTT) (gels on the right). B Band quantification of Western blot detected proteins. Samples collected at 4, 6 and 8 dpi. Four leaf discs from different leaves were collected from each biological sample. 20 μL TSP of PBST0.1 % treated sample or equivalent volume of SDS-DTT treated sample were loaded on the gel. Black arrows denote monomeric G-CSF:eYFP variant. p19: negative control. eGEHK: protein standard. Proteins were detected with GFP antibody. Band quantification of Western blot detected proteins (Graph). Columns denoted with a different letter are significantly different (p ≤ 0.05) using one-way ANOVA and followed by Tukey test. Error bars are standard deviation of the means

Maydica, 2019

High oil maize (HOM) hybrids, adapted to subtropical regions are needed to meet existing requirem... more High oil maize (HOM) hybrids, adapted to subtropical regions are needed to meet existing requirements for industrial and livestock purposes. Recently, the doubled haploid (DH) breeding technology is applied to reduce considerably the time to generate homozygous maize lines, however, information of heterotic response, per se productivity, stress, disease reaction, and adaptation to different environments are not known. In this work, field evaluations of per se and testcross high oil content DH lines adapted for subtropical environments were studied to identify superior DH lines with good heterotic response and agronomic characteristics. White and yellow kernel test crosses were formed using the different heterotic population as testers from which DH lines were derived. Test crosses were evaluated at three locations in Mexico. Superior DH lines from each tester presented high GCA effects through test crosses, with a range of grain yield across locations of 12.8 to 10.4 Mg ha-1 and 11....

Journal of Stored Products Research, 2019

Postharvest grain storage are a major problem in Mexico, influencing the economy, livelihoods, an... more Postharvest grain storage are a major problem in Mexico, influencing the economy, livelihoods, and food security of most farmers. At present, very limited information is available on postharvest maize losses and the associated insect pests in Mexico. Therefore, the objective of this study was to quantify and map maize storage losses in Central Mexico (State of Mexico) to analyze the effects of major pests in the different regions and to provide useful data to policymakers and local stakeholders. The study was conducted with 120 farmers dispersed across all regions of maize production in the State of Mexico. Storage losses were quantified using standardized maize samples that harvest, stored for a year, and sampled periodically. These data, together with geographic coordinates, were integrated into a geographic information system (GIS) to generate maps of maize storage damage and weight losses. The resulting maps show that in the southern region of the State of Mexico the standardized samples exhibited the highest maize losses after a one-year storage period, with an average of 76% loss and 100% grain damage, followed by the northern region, with an average of 18% loss and 52% damage. The eastern region reported 10% loss and 16% damage, whereas the Central region showed 5% loss and 14% damage. The main storage pests identified displayed localized geographic distributions, with the maize weevil, Sitophilus zeamais, being mainly localized in the South, the larger grain borer, Prostephanus truncatu, in the East and North, and the angoumois grain moth, Sitotroga cerealella, in the Northeast and Central regions. Thus, these maps are robust tools that will help towards improving storage facilities and increasing food security for small-scale farmers.

Journal of Cereal Science, 2018

Teosinte is the wild ancestor of modern maize. It has served as a study model of evolutionary pro... more Teosinte is the wild ancestor of modern maize. It has served as a study model of evolutionary processes and even more as a potential source of genomic variation to introgress maize varieties suitable for both food and feed. Although some metabolites have been studied in teosinte, little is known about its secondary metabolites like phenolic compounds. To evaluate the diversity present in teosinte, 22 different teosinte varieties were used to extract and quantify soluble and bound total and specific phenolic compounds by HPLC-PDA, respectively. Also, their antioxidant bioactivities were evaluated. Kernel biophysical parameters showed significant differences among the different teosinte varieties. Teosinte's phenolic profile showed that pcoumaric acid was the predominant phenolic acid form with soluble concentrations around 17 µg/gdw to 31 µg/gdw whereas concentrations of bound ranged from 215 to 238 µg/gdw in all varieties. Teosinte's antioxidant capacities were higher for bound phenolics with values ranging from 50 to 82 mmolTE/100gdw compared to only 7 to 17 mmolTE/100gdw observed in commercial maize. The diversity found in phenolic content and potential bioactivity of teosinte could serve as a platform for the development of new breeding programs into high performance modern maize with distinct nutraceutical potential.

Food Chemistry, 2018

The objective of this work was to assess the effects of a traditional parboiling treatment on phy... more The objective of this work was to assess the effects of a traditional parboiling treatment on physical, chemical and functional properties of yellow maize kernels. For this, maize kernels were subjected to the three main stages of a traditional parboiling process (soaking, steaming, and drying) at different moisture contents (15%, 25%, or 35%), and different pressure steaming times (0, 15, or 30 min). Kernels were evaluated for physical and chemical changes, while manually generated endosperm fractions were further evaluated for nutritional and functional changes. The parboiling process negatively altered the maize kernels properties by increasing the number of kernels with burst pericarp and decreasing the total carotenoid content in the endosperm by 42%. However, the most intense conditions (35% moisture and 30 min steam) lowered the number of broken kernels by 41%, and the number of stress cracks by 36%. Results also demonstrated that soaking enhanced the nutritional value of soaked yellow maize by increasing the thiamine content and the bound phenolic content in the endosperm fraction up to 102%. The proper implementation of this hydrothermal treatment could lead to significant enhancements in nutritional and functionality of maize products.

Molecules

The development of novel cancer therapeutic strategies has garnered increasing interest in cancer... more The development of novel cancer therapeutic strategies has garnered increasing interest in cancer research. Among the therapeutic choices, chemosensitizers have shown exciting prospects. Peptides are an attractive alternative among the molecules that may be used as chemosensitizers. We rationally designed a new-to-nature peptide, nurP28, derived from the 22-kDa α-zein protein sequence (entry Q00919_MAIZE). The resultant sequence of the nurP28 peptide after the addition of arginine residues was LALLALLRLRRRATTAFIIP, and we added acetyl and amide groups at the N- and C-terminus, respectively, for capping. We evaluated the cytotoxicity of the nurP28 peptide alone and in combination with docetaxel in fibroblast monolayers and breast cancer monolayers and spheroids. Our results indicated that nurP28 is not cytotoxic to human fibroblasts or cancer cells. Nevertheless, when combined with 1 µM docetaxel, 3 ng/mL nurP28 induced equivalent (in MCF7 monolayers) and higher (in MCF7 spheroids) c...

Corn, 2019

Abstract The corn kernel is the fruit or caryopsis borne in the female inflorescence of the plant... more Abstract The corn kernel is the fruit or caryopsis borne in the female inflorescence of the plant, which contains the embryo and storage tissues designed to create upon germination the new seedling and generation plant. Kernels are constituted by three major anatomical parts: pericarp, germ, and pericarp and contain high levels of starch, protein, oil, and other nutritionally valuable substances for humankind. Within each type of corn (see Chapter 10 ), important variations exist in endosperm hardness, pericarp, endosperm color, type of starch, and kernel size. In order to understand the relevant changes that corn undergoes during processing, it is essential to comprehend the macro- and microstructure and the basic composition of each anatomical part of the caryopsis. Better understanding of the macro- and microstructural features and properties of corn improves our ability to utilize it in many food and industrial products. It is also necessary to achieve optimal quality preservation in harvesting, drying, storing, and processing. Furthermore, the structure and the appearance of modern corn have been modified by breeding and the knowledge of the relationships among structural component is fundamental for plant breeding, wet and dry-milling, and food processors. This chapter reviews relevant information on the physical properties of the corn kernel and the constitution and functionality of its anatomical parts and is mainly focused on to establish a solid foundation of the current state of knowledge of the anatomy and architecture of the corn kernel. Ultimately, the understanding of the microstructural features of the anatomical parts of the kernel is of utmost importance for all industrial segments because they are closely related to yield and quality of end products.

Journal of Stored Products Research, 2013

ABSTRACT The aim of this research was to compare the processing and quality of tortillas produced... more ABSTRACT The aim of this research was to compare the processing and quality of tortillas produced from two kinds of larger grain borer (LGB) Prostephanus truncatus (Horn.) damaged kernels: resistant (IRM) and susceptible (ISM) genotypes. The damaged LGB kernels had significant lower test weight, 1000 kernel weigh and density. The ISM kernels were more negatively affected by insects compared with the IRM counterpart. A significant reduction of 5% in starch was observed in IRM kernels but not in ISM counterparts. Flour acidity and protein increased 8-fold and 5%, respectively parallel to an augmentation of weight grain losses whereas the crude fat content significantly reduced by 29%. Insect damage enhanced the penetration of the hot lime solution into the starchy endosperm. Insect infested kernels which lost 10% and 20% of their weight required 34% and 42% less lime-cooking time compared to sound kernels. The 10% and 20% insect-damaged kernels lost 15 and 23% of their solids during storage and tortilla processing, respectively. Finally, LGB damaged kernels reduced substantially the tortilla quality in terms of color.

Cereal Chemistry, 2018

Background and objectives: Commercial yellow corn contains approximately 4% oil whereas improved ... more Background and objectives: Commercial yellow corn contains approximately 4% oil whereas improved high-oil corn (HOC) genotypes from 6 to 9% oil. The higher oil increases digestible energy and protein content. Improved HOC grains of white and yellow corn hybrids adapted to subtropical ecosystems were analyzed for total oil content and fatty acid composition. The anatomical properties of kernels were determined, and total oil content and fatty acid compositions were assessed. Findings: Significant differences were observed among white and yellow hybrids in terms of oil content and fatty acids. The oil content in yellow and white hybrids ranged from 6% to 9% and from 5% to 8%, respectively. The oil content was positively correlated with the size of the germ (r=0.55 p<0.001) and negatively correlated with that of the endosperm (r=-0.484 p<0.001). Oleic acid (OLA) comprised 36% to 51% of the total fatty acids (FAs) whereas linoleic acid (LOA) in yellow and white hybrids ranged from 35% to 52%, and from 37% to 50% respectively.

Additional file 3. Comparison of free phenolic acids determination by conventional and improved m... more Additional file 3. Comparison of free phenolic acids determination by conventional and improved methods. *Results are expressed as the average of three replicates as µg per g dry weight (µg/g dw). Min: minimum value of the data, Max: maximum value of the data. LSD: Least significant difference (α = 0.05).

Additional file 2. Comparison of total phenolic acids determination by conventional and improved ... more Additional file 2. Comparison of total phenolic acids determination by conventional and improved methods. *Results are expressed as the average of three replicates as mg of gallic acid equivalent per 100 g dry weight (mg GAE/100 g dw). Min: minimum value of the data, Max: maximum value of the data. LSD: Least significant difference (α = 0.05).