Rabiul Islam | Islamic University, Kushtia,Bangladesh (original) (raw)

Papers by Rabiul Islam

University of Mohammed Premier Oujda Morocco, 2020

The genetic modification of an organism involves transferring DNA, the genetic component from a p... more The genetic modification of an organism involves transferring DNA, the genetic component from a plant or bacterium, or even an animal, into a different organism. The aim of the review study to find out the positive and negative effect of GM crops on human health and environment finally awareness to the people to acceptance the GM crops it beneficial effect from lowest hazard. Opportunity presented by proponents of
GM technology include development in fruit and vegetable shelf- life and organoleptic quality, prosperous nutritional quality and health benefits in foods, elevated protein and carbohydrate content of foods, exalted fat quality, high quality and quantity of meat, milk and livestock. We study various type of review article on GM foods and findings the positive effect it’s the methodology of this paper. In addition, some more common concerns include environmental pollution, unintentional gene alteration to wild plants, probable creation of new viruses and toxins, restricted access to seeds due to patenting of GM food plants, impedance to crop genetic diversity, religious, cultural and ethical
concerns, as well as fear of the unknown. Boosters of GM technology include private industries, research scientists, some consumers, U.S. farmers and regulatory agencies. The result of the study minimizing potential risks and maximizing the profits of GM foods finally it should be approval for us. Because the advantages of GM foods ostensibly far out weight the risks, regulatory agencies and industries engaged in GM
food business. Finally increment public awareness in this technology to prolong worldwide acceptability of GM foods will be reducing in future of food crisis in the world.

Journal of Chemical, Biological and Physical Sciences , 2020

Urinary tract infections (UTIs) in humans are very common in Bangladesh. The aims and objectives ... more Urinary tract infections (UTIs) in humans are very common in Bangladesh. The aims and objectives of this study were to identify the urinary tract pathogens by utilizing conventional culture media and in vitro antimicrobial sensitivity profiles by the Kirby- Bauer disc diffusion method. A total of 268 urine samples were aseptically collected for this study. The cultural analysis was done by phenotypic examination to identify the isolates. Finally, antibiotic sensitivity was tested against conventionally used antibiotics. Only 19.4% of the urine samples yielded a positive culture. UTI showed more prevalence among females than males. The age group of 13-48 years was found as a high risk for UTIs. In this study, the most predominant isolate was E. coli 50(96.2%) and the rest of isolates were Klebsiella pneumoniae 1(1.9%) and Pseudomonas aeruginosa 1(1.9%). Like most of the previous reports, E. coli was predominant, which was also corroborated in our study. However, our investigation generated an antimicrobial profile of the detected organisms that showed variations comparing with previous studies. The isolates were found resistant to the most common oral antibiotics used, such as cotrimoxazole, nalidixic acid, cephradine, Ceftriaxone, and so on. The pathogens showed a remarkable amount of sensitivity against gentamicin, imipenem, meropenem. For validating more reliability, this research needs further works.

International Journal of Current Research, 2020

Nutrient deficiency in the soil poses a big challenge to food production globally. The use of art... more Nutrient deficiency in the soil poses a big challenge to food production globally. The use of artificial nitrogen fertilizer to aid crop yield is a common farming practice, despite its undesirable effects and hazard to the environment and human population. This research work aimed at isolation, identification & characterization of Rhizobium species from chickpea rhizospheric soil samples collected of the southern region of faridpur district in Bangladesh. Isolation of Rhizobium species was culture on Yeast Extract Mannitol Agar (YEMA) medium incubated 3 days at 320C. A total of 10 Rhizobium species isolates were isolated from rhizospheric soil samples. They are also found to be gram-negative, rod-shaped morphology, fast grower, indole producers and positive for catalase test. All isolates were found with bare absorption of Congo red dye & no growth on YEMA with 2% NaCl. Out of 10 only 3 isolates (FSRS-3, FSRS-7, and FSRS-9) were identified as Rhizobium species on the basis of the authentication test (nodulation check with (Cicer arietinum). These three rhizobial isolates may be useful to increase the symbiotic biological nitrogen fixation in legume plant chickpea (Cicer arietinum) and can be used as potential biofertilizer owing to their plant growth-promoting characters.

European Journal of Medical and Health Sciences , 2020

Cissus quadrangularis (Vitaceae) is a popular climber conspicuous by its flesh quadrangular stem ... more Cissus quadrangularis (Vitaceae) is a popular climber conspicuous by its flesh quadrangular stem widespread throughout the Bangladesh. The in vitro antimicrobial activity of C. quadrangularis extracts was studied against selected pathogenic bacteria, industrial wasted bacteria, and broth dilution assay. The most commonly used method of microbiological assay is the disc diffusion method. C. quadrangularis stem extracted with four solvents (Petroleum spirit, methanol, ethyl acetate, and dichloromethane) were tested for antimicrobial activities against some pathogenic microorganisms Sarcina lutea (002-1), Xanthomonas campestris (004-1), Escherichia coli (005-1), Klebsiella pneumonia (006-1) and some industrial (Tannery, Tobacco, and Sugar mill) waste watered bacteria by disc diffusion method. Among the four extracts, ethyl acetate showed moderate antibacterial activity against X. campestris (004-1) and industrial watered bacteria. But, the commercial disc Oxicycline doesn’t show any antibacterial activity against the industrial waste watered bacteria. Petroleum spirit, methanol, and dichloromethane extract were ineffective against all of the tested bacteria.
Keywords

Advances in Bioscience and Biotechnology, 2020

Biopreservation systems in foods are of increasing interest for industry and consumers. Bacterioc... more Biopreservation systems in foods are of increasing interest for industry and consumers. Bacteriocin producing Lactobacillus spp. is considered Generally Recognize as Safe (GRAS), useful to control the fast development of pathogens and spoiling microbes in food and feed. Lactobacillus spp. were isolated from traditional winter fermented vegetable cucumber & carrot by the use of selective media. Especially De Man, Rogosa and Sharpe (MRS) Agar media were used to isolate the Lactobacillus species. Morphologically identify by gram staining & colony morphology. Biochemically recognize by catalase, oxidase, MRVP & carbohydrate fermentation test. Antimicrobial activity of Lactobacillus spp. was confirmed by Well Diffusion Method. Molecular characterization of bacteriocin protein and molecular weight determination by SDS PAGE method. The isolate were found to be facultative anaerobic, Gram-positive, and catalase negative. The result of antimicrobial activity measurement by the Arbitrary Unit (AU/ml) of zone of the inhibition. Six isolate found from the sample but most activities exhibited isolate 4 against Bacillus megaterium (55 mm) zone of diameter. The molecular weight of the washed bacteriocin was calculated to be about 40 kDa (Isolate 1) and 15 kDa & 30 kDa (Isolate 5). Bacteriocin protein reduces chemical preservatives and uses in future as biopreservative in food industry.

American Journal of Pure and Applied Biosciences , 2019

ABSTRACT Pathogenic microorganisms are major health concern of infectious diseases. In the presen... more ABSTRACT Pathogenic microorganisms are major health concern of infectious diseases. In the present study ethanolic and methanolic extracts of Cassia obtusifolia leaves from Kushtia region (Bangladesh) were subjected to evaluate the in vitro microbial activity against six important human pathogenic bacteria viz., Bacillus subtilis (001-1), Sarcina lutea (002-1), Xanthomonas campestris (004-1), Escherichia coli (005-1), Klebsiella pneumonia (006-1) and Pseudomonas sp. (010-1) employing disc diffusion method. The crude methanolic extract of C. obtusifolia produced maximum area of inhibition (14 mm) against S. lutea (002-1) and crude ethanolic extract of C. obtusifolia produced largest area of inhibition (11 mm) against K. pneumonia (006-1). The MIC values (256 μg/ml, 512 μg/ml) were obtained from the methanolic isolate and ethanolic extract that produced 4 mm and 3 mm area of inhibition against S. lutea (002-1) and K. pneumonia (006-1). Methanol extract showed the greater activity than ethanol extract. The most susceptible bacterial strains to ethanol and methanol extracts were S. lutea (002-1) and K. pneumonia (006-1). So it may be possible that the production of new antibiotic from C. obtusifolia L. leaf may be recommended for meningitis and pneumonia. The findings of this research suggest that the extracts of C. obtusifolia L. can be a source of natural antibacterial agents with pivotal applications in pharmaceutical companies to control pathogenic bacteria causing severe illness in humans.
Keywords

University of Mohammed Premier Oujda Morocco, 2020

The genetic modification of an organism involves transferring DNA, the genetic component from a p... more The genetic modification of an organism involves transferring DNA, the genetic component from a plant or bacterium, or even an animal, into a different organism. The aim of the review study to find out the positive and negative effect of GM crops on human health and environment finally awareness to the people to acceptance the GM crops it beneficial effect from lowest hazard. Opportunity presented by proponents of
GM technology include development in fruit and vegetable shelf- life and organoleptic quality, prosperous nutritional quality and health benefits in foods, elevated protein and carbohydrate content of foods, exalted fat quality, high quality and quantity of meat, milk and livestock. We study various type of review article on GM foods and findings the positive effect it’s the methodology of this paper. In addition, some more common concerns include environmental pollution, unintentional gene alteration to wild plants, probable creation of new viruses and toxins, restricted access to seeds due to patenting of GM food plants, impedance to crop genetic diversity, religious, cultural and ethical
concerns, as well as fear of the unknown. Boosters of GM technology include private industries, research scientists, some consumers, U.S. farmers and regulatory agencies. The result of the study minimizing potential risks and maximizing the profits of GM foods finally it should be approval for us. Because the advantages of GM foods ostensibly far out weight the risks, regulatory agencies and industries engaged in GM
food business. Finally increment public awareness in this technology to prolong worldwide acceptability of GM foods will be reducing in future of food crisis in the world.

Journal of Chemical, Biological and Physical Sciences , 2020

Urinary tract infections (UTIs) in humans are very common in Bangladesh. The aims and objectives ... more Urinary tract infections (UTIs) in humans are very common in Bangladesh. The aims and objectives of this study were to identify the urinary tract pathogens by utilizing conventional culture media and in vitro antimicrobial sensitivity profiles by the Kirby- Bauer disc diffusion method. A total of 268 urine samples were aseptically collected for this study. The cultural analysis was done by phenotypic examination to identify the isolates. Finally, antibiotic sensitivity was tested against conventionally used antibiotics. Only 19.4% of the urine samples yielded a positive culture. UTI showed more prevalence among females than males. The age group of 13-48 years was found as a high risk for UTIs. In this study, the most predominant isolate was E. coli 50(96.2%) and the rest of isolates were Klebsiella pneumoniae 1(1.9%) and Pseudomonas aeruginosa 1(1.9%). Like most of the previous reports, E. coli was predominant, which was also corroborated in our study. However, our investigation generated an antimicrobial profile of the detected organisms that showed variations comparing with previous studies. The isolates were found resistant to the most common oral antibiotics used, such as cotrimoxazole, nalidixic acid, cephradine, Ceftriaxone, and so on. The pathogens showed a remarkable amount of sensitivity against gentamicin, imipenem, meropenem. For validating more reliability, this research needs further works.

International Journal of Current Research, 2020

Nutrient deficiency in the soil poses a big challenge to food production globally. The use of art... more Nutrient deficiency in the soil poses a big challenge to food production globally. The use of artificial nitrogen fertilizer to aid crop yield is a common farming practice, despite its undesirable effects and hazard to the environment and human population. This research work aimed at isolation, identification & characterization of Rhizobium species from chickpea rhizospheric soil samples collected of the southern region of faridpur district in Bangladesh. Isolation of Rhizobium species was culture on Yeast Extract Mannitol Agar (YEMA) medium incubated 3 days at 320C. A total of 10 Rhizobium species isolates were isolated from rhizospheric soil samples. They are also found to be gram-negative, rod-shaped morphology, fast grower, indole producers and positive for catalase test. All isolates were found with bare absorption of Congo red dye & no growth on YEMA with 2% NaCl. Out of 10 only 3 isolates (FSRS-3, FSRS-7, and FSRS-9) were identified as Rhizobium species on the basis of the authentication test (nodulation check with (Cicer arietinum). These three rhizobial isolates may be useful to increase the symbiotic biological nitrogen fixation in legume plant chickpea (Cicer arietinum) and can be used as potential biofertilizer owing to their plant growth-promoting characters.

European Journal of Medical and Health Sciences , 2020

Cissus quadrangularis (Vitaceae) is a popular climber conspicuous by its flesh quadrangular stem ... more Cissus quadrangularis (Vitaceae) is a popular climber conspicuous by its flesh quadrangular stem widespread throughout the Bangladesh. The in vitro antimicrobial activity of C. quadrangularis extracts was studied against selected pathogenic bacteria, industrial wasted bacteria, and broth dilution assay. The most commonly used method of microbiological assay is the disc diffusion method. C. quadrangularis stem extracted with four solvents (Petroleum spirit, methanol, ethyl acetate, and dichloromethane) were tested for antimicrobial activities against some pathogenic microorganisms Sarcina lutea (002-1), Xanthomonas campestris (004-1), Escherichia coli (005-1), Klebsiella pneumonia (006-1) and some industrial (Tannery, Tobacco, and Sugar mill) waste watered bacteria by disc diffusion method. Among the four extracts, ethyl acetate showed moderate antibacterial activity against X. campestris (004-1) and industrial watered bacteria. But, the commercial disc Oxicycline doesn’t show any antibacterial activity against the industrial waste watered bacteria. Petroleum spirit, methanol, and dichloromethane extract were ineffective against all of the tested bacteria.
Keywords

Advances in Bioscience and Biotechnology, 2020

Biopreservation systems in foods are of increasing interest for industry and consumers. Bacterioc... more Biopreservation systems in foods are of increasing interest for industry and consumers. Bacteriocin producing Lactobacillus spp. is considered Generally Recognize as Safe (GRAS), useful to control the fast development of pathogens and spoiling microbes in food and feed. Lactobacillus spp. were isolated from traditional winter fermented vegetable cucumber & carrot by the use of selective media. Especially De Man, Rogosa and Sharpe (MRS) Agar media were used to isolate the Lactobacillus species. Morphologically identify by gram staining & colony morphology. Biochemically recognize by catalase, oxidase, MRVP & carbohydrate fermentation test. Antimicrobial activity of Lactobacillus spp. was confirmed by Well Diffusion Method. Molecular characterization of bacteriocin protein and molecular weight determination by SDS PAGE method. The isolate were found to be facultative anaerobic, Gram-positive, and catalase negative. The result of antimicrobial activity measurement by the Arbitrary Unit (AU/ml) of zone of the inhibition. Six isolate found from the sample but most activities exhibited isolate 4 against Bacillus megaterium (55 mm) zone of diameter. The molecular weight of the washed bacteriocin was calculated to be about 40 kDa (Isolate 1) and 15 kDa & 30 kDa (Isolate 5). Bacteriocin protein reduces chemical preservatives and uses in future as biopreservative in food industry.

American Journal of Pure and Applied Biosciences , 2019

ABSTRACT Pathogenic microorganisms are major health concern of infectious diseases. In the presen... more ABSTRACT Pathogenic microorganisms are major health concern of infectious diseases. In the present study ethanolic and methanolic extracts of Cassia obtusifolia leaves from Kushtia region (Bangladesh) were subjected to evaluate the in vitro microbial activity against six important human pathogenic bacteria viz., Bacillus subtilis (001-1), Sarcina lutea (002-1), Xanthomonas campestris (004-1), Escherichia coli (005-1), Klebsiella pneumonia (006-1) and Pseudomonas sp. (010-1) employing disc diffusion method. The crude methanolic extract of C. obtusifolia produced maximum area of inhibition (14 mm) against S. lutea (002-1) and crude ethanolic extract of C. obtusifolia produced largest area of inhibition (11 mm) against K. pneumonia (006-1). The MIC values (256 μg/ml, 512 μg/ml) were obtained from the methanolic isolate and ethanolic extract that produced 4 mm and 3 mm area of inhibition against S. lutea (002-1) and K. pneumonia (006-1). Methanol extract showed the greater activity than ethanol extract. The most susceptible bacterial strains to ethanol and methanol extracts were S. lutea (002-1) and K. pneumonia (006-1). So it may be possible that the production of new antibiotic from C. obtusifolia L. leaf may be recommended for meningitis and pneumonia. The findings of this research suggest that the extracts of C. obtusifolia L. can be a source of natural antibacterial agents with pivotal applications in pharmaceutical companies to control pathogenic bacteria causing severe illness in humans.
Keywords