Dr-Abdul Ghaffar | The Islamia University of Bahawalpur (original) (raw)
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Papers by Dr-Abdul Ghaffar
Pakistan Veterinary Journal, 2014
Received: Revised: Accepted: September 10, 2013 October 11, 2013 October 23, 2013 The present exp... more Received: Revised: Accepted: September 10, 2013 October 11, 2013 October 23, 2013 The present experimental study was conducted to determine the clinico-hematological changes induced by oral administration of triazophos in male Japanese quail. A total of 60 mature male quail were procured and randomly divided into five equal groups. All the experimental birds received triazophos orally @ 0, 2, 4, 6 and 8 mg/kg/day for 48 days. Birds were killed at day 16, 32 and 48 for collection of blood. Various clinical signs like ruffled feathers, tremors, watery droppings, salivation, torticollis, less foam production, decreased frequency of crowing and mating with their pen mates were observed at high doses (6 and 8 mg/kg/day triazophos). Significantly (P<0.05) lowered feed intake and body weight was recorded. Overall significantly (P<0.05) decreased total erythrocyte counts, total leukocyte counts, hemoglobin concentration, mean corpuscular hemoglobin concentration while increased pack c...
Received: Revised: Accepted: May 17, 2014 February 07, 2015 February 10, 2015 The present study w... more Received: Revised: Accepted: May 17, 2014 February 07, 2015 February 10, 2015 The present study was conducted to explore the clinico-hematological and genotoxic effects in fresh water fish (Labeo rohita) exposed to various concentrations of butachlor (chloroacetanilide herbicide). For this purpose 30 fish weighing about 500-600 g were randomly allocated to five equal groups (A-E) and treated with various concentrations (0, 0.25, 0.50, 0.75 and 1.00 mg/L) of butachlor. Blood samples were collected from each fish after 48, 72 and 96 h of post treatment and fresh thin blood smears were made for cellular abnormalities. All the fish kept in group A did not reveal any clinical and behavioral ailments. However, various clinical signs such as rapid breathing, gasping at the water surface, restlessness, opercular movement, loss of equilibrium and erratic swimming were observed at higher concentrations (0.75 and 1.00 mg/L) of herbicide. The results showed that the erythrocyte, hemoglobin, hem...
International Journal of Agriculture and Biology, Apr 1, 2015
Extensive use of pesticides in agriculture sector represents a major proportion of pollutants and... more Extensive use of pesticides in agriculture sector represents a major proportion of pollutants and poses serious threats to both environment and aquatic organisms. In the present study micronucleus assay and nuclear abnormalities were used as a biomarker to assess the cyto-genotoxic potential of different concentrations of triazophos insecticide in fresh water fish (Labeo rohita L.). For this purpose 16 fresh water fish of same age and weight were kept into four equal groups (A-D) having four each. All the fish were kept in aquaria with 75 L water capacity for six days for acclimatization. Various sub-lethal concentrations of organophosphate insecticide triazophos (0.010, 0.015 and 0.200 ppm) were tested. For hematological and cyto-genotoxic studies blood samples were collected from each fish after 48, 72 and 96 h of post-treatment. Duplicate thin blood smear was made from fresh blood of each fish. Results revealed that total erythrocyte count, pack cell volume, hemoglobin concentration, serum total proteins, mean corpuscular hemoglobin concentration and mean corpuscular volume values were significantly (P<0.05) decreased, which indicated that fish were suffering from microcytic hypochromic anemia. Lymphocyte and monocyte values were also significantly decreased while leukocyte count was significantly increased. The results obtained by micronucleus assay showed significantly a higher frequency of erythrocyte with micronuclei, blebbed nuclei, lobed, notched, heteropicnotic nuclei, binucleated and pear shape erythrocyte. The serum analysis showed that the concentration of different enzymes and lipid peroxidation products were significantly increased in exposed fish. It can be concluded that triazophos poses adverse hemato-biochemical and DNA damage effects in aquatic organisms.
Extensive use of pesticides in agriculture sector represents a major proportion of pollutants and... more Extensive use of pesticides in agriculture sector represents a major proportion of pollutants and poses serious threats to both environment and aquatic organisms. In the present study micronucleus assay and nuclear abnormalities were used as a biomarker to assess the cyto-genotoxic potential of different concentrations of triazophos insecticide in fresh water fish (Labeo rohita L.). For this purpose 16 fresh water fish of same age and weight were kept into four equal groups (A-D) having four each. All the fish were kept in aquaria with 75 L water capacity for six days for acclimatization. Various sub-lethal concentrations of organophosphate insecticide triazophos (0.010, 0.015 and 0.200 ppm) were tested. For hematological and cyto-genotoxic studies blood samples were collected from each fish after 48, 72 and 96 h of post-treatment. Duplicate thin blood smear was made from fresh blood of each fish. Results revealed that total erythrocyte count, pack cell volume, hemoglobin concentration, serum total proteins, mean corpuscular hemoglobin concentration and mean corpuscular volume values were significantly (P<0.05) decreased, which indicated that fish were suffering from microcytic hypochromic anemia. Lymphocyte and monocyte values were also significantly decreased while leukocyte count was significantly increased. The results obtained by micronucleus assay showed significantly a higher frequency of erythrocyte with micronuclei, blebbed nuclei, lobed, notched, heteropicnotic nuclei, binucleated and pear shape erythrocyte. The serum analysis showed that the concentration of different enzymes and lipid peroxidation products were significantly increased in exposed fish. It can be concluded that triazophos poses adverse hemato-biochemical and DNA damage effects in aquatic organisms.
The present study was conducted to explore the clinico-hematological and genotoxic effects in fre... more The present study was conducted to explore the clinico-hematological and
genotoxic effects in fresh water fish (Labeo rohita) exposed to various
concentrations of butachlor (chloroacetanilide herbicide). For this purpose 30 fish
weighing about 500-600 g were randomly allocated to five equal groups (A-E) and
treated with various concentrations (0, 0.25, 0.50, 0.75 and 1.00 mg/L) of butachlor.
Blood samples were collected from each fish after 48, 72 and 96 h of post treatment
and fresh thin blood smears were made for cellular abnormalities. All the fish kept
in group A did not reveal any clinical and behavioral ailments. However, various
clinical signs such as rapid breathing, gasping at the water surface, restlessness,
opercular movement, loss of equilibrium and erratic swimming were observed at
higher concentrations (0.75 and 1.00 mg/L) of herbicide. The results showed that
the erythrocyte, hemoglobin, hematocrit and lymphocyte values were significantly
(P<0.05) decreased while significantly increased total leukocyte counts were
recorded. Significantly increased incidence of different morphological and nuclear
abnormalities such as pear shape erythrocyte, microcyte, tear shape erythrocyte,
erythrocytes with micronuclei, lobed, blebbed and notched nuclei and cells with
nuclear remnants were observed. The results suggested that butachlor poses serious
clinico-hematological and mutagenic effects in fish and may be hazardous to the
other species when those are exposed to this herbicide.
The present experimental study was conducted to determine the clinico-hematological changes induc... more The present experimental study was conducted to determine the clinico-hematological changes induced by oral administration of triazophos in male Japanese quail. A total of 60 mature male quail were procured and randomly divided into five equal groups. All the experimental birds received triazophos orally @ 0, 2, 4, 6 and 8 mg/kg/day for 48 days. Birds were killed at day 16, 32 and 48 for collection of blood. Various clinical signs like ruffled feathers, tremors, watery droppings, salivation, torticollis, less foam production, decreased frequency of crowing and mating with their pen mates were observed at high doses (6 and 8 mg/kg/day triazophos). Significantly (P<0.05) lowered feed intake and body weight was recorded. Overall significantly (P<0.05) decreased total erythrocyte counts, total leukocyte counts, hemoglobin concentration, mean corpuscular hemoglobin concentration while increased pack cell volume and mean corpuscular volume was recorded in treated birds. It was concluded that triazophos induces clinico-hematological changes including anemia (macrocytic hypochromic) at higher doses in birds.
Pakistan Veterinary Journal, 2014
Received: Revised: Accepted: September 10, 2013 October 11, 2013 October 23, 2013 The present exp... more Received: Revised: Accepted: September 10, 2013 October 11, 2013 October 23, 2013 The present experimental study was conducted to determine the clinico-hematological changes induced by oral administration of triazophos in male Japanese quail. A total of 60 mature male quail were procured and randomly divided into five equal groups. All the experimental birds received triazophos orally @ 0, 2, 4, 6 and 8 mg/kg/day for 48 days. Birds were killed at day 16, 32 and 48 for collection of blood. Various clinical signs like ruffled feathers, tremors, watery droppings, salivation, torticollis, less foam production, decreased frequency of crowing and mating with their pen mates were observed at high doses (6 and 8 mg/kg/day triazophos). Significantly (P<0.05) lowered feed intake and body weight was recorded. Overall significantly (P<0.05) decreased total erythrocyte counts, total leukocyte counts, hemoglobin concentration, mean corpuscular hemoglobin concentration while increased pack c...
Received: Revised: Accepted: May 17, 2014 February 07, 2015 February 10, 2015 The present study w... more Received: Revised: Accepted: May 17, 2014 February 07, 2015 February 10, 2015 The present study was conducted to explore the clinico-hematological and genotoxic effects in fresh water fish (Labeo rohita) exposed to various concentrations of butachlor (chloroacetanilide herbicide). For this purpose 30 fish weighing about 500-600 g were randomly allocated to five equal groups (A-E) and treated with various concentrations (0, 0.25, 0.50, 0.75 and 1.00 mg/L) of butachlor. Blood samples were collected from each fish after 48, 72 and 96 h of post treatment and fresh thin blood smears were made for cellular abnormalities. All the fish kept in group A did not reveal any clinical and behavioral ailments. However, various clinical signs such as rapid breathing, gasping at the water surface, restlessness, opercular movement, loss of equilibrium and erratic swimming were observed at higher concentrations (0.75 and 1.00 mg/L) of herbicide. The results showed that the erythrocyte, hemoglobin, hem...
International Journal of Agriculture and Biology, Apr 1, 2015
Extensive use of pesticides in agriculture sector represents a major proportion of pollutants and... more Extensive use of pesticides in agriculture sector represents a major proportion of pollutants and poses serious threats to both environment and aquatic organisms. In the present study micronucleus assay and nuclear abnormalities were used as a biomarker to assess the cyto-genotoxic potential of different concentrations of triazophos insecticide in fresh water fish (Labeo rohita L.). For this purpose 16 fresh water fish of same age and weight were kept into four equal groups (A-D) having four each. All the fish were kept in aquaria with 75 L water capacity for six days for acclimatization. Various sub-lethal concentrations of organophosphate insecticide triazophos (0.010, 0.015 and 0.200 ppm) were tested. For hematological and cyto-genotoxic studies blood samples were collected from each fish after 48, 72 and 96 h of post-treatment. Duplicate thin blood smear was made from fresh blood of each fish. Results revealed that total erythrocyte count, pack cell volume, hemoglobin concentration, serum total proteins, mean corpuscular hemoglobin concentration and mean corpuscular volume values were significantly (P<0.05) decreased, which indicated that fish were suffering from microcytic hypochromic anemia. Lymphocyte and monocyte values were also significantly decreased while leukocyte count was significantly increased. The results obtained by micronucleus assay showed significantly a higher frequency of erythrocyte with micronuclei, blebbed nuclei, lobed, notched, heteropicnotic nuclei, binucleated and pear shape erythrocyte. The serum analysis showed that the concentration of different enzymes and lipid peroxidation products were significantly increased in exposed fish. It can be concluded that triazophos poses adverse hemato-biochemical and DNA damage effects in aquatic organisms.
Extensive use of pesticides in agriculture sector represents a major proportion of pollutants and... more Extensive use of pesticides in agriculture sector represents a major proportion of pollutants and poses serious threats to both environment and aquatic organisms. In the present study micronucleus assay and nuclear abnormalities were used as a biomarker to assess the cyto-genotoxic potential of different concentrations of triazophos insecticide in fresh water fish (Labeo rohita L.). For this purpose 16 fresh water fish of same age and weight were kept into four equal groups (A-D) having four each. All the fish were kept in aquaria with 75 L water capacity for six days for acclimatization. Various sub-lethal concentrations of organophosphate insecticide triazophos (0.010, 0.015 and 0.200 ppm) were tested. For hematological and cyto-genotoxic studies blood samples were collected from each fish after 48, 72 and 96 h of post-treatment. Duplicate thin blood smear was made from fresh blood of each fish. Results revealed that total erythrocyte count, pack cell volume, hemoglobin concentration, serum total proteins, mean corpuscular hemoglobin concentration and mean corpuscular volume values were significantly (P<0.05) decreased, which indicated that fish were suffering from microcytic hypochromic anemia. Lymphocyte and monocyte values were also significantly decreased while leukocyte count was significantly increased. The results obtained by micronucleus assay showed significantly a higher frequency of erythrocyte with micronuclei, blebbed nuclei, lobed, notched, heteropicnotic nuclei, binucleated and pear shape erythrocyte. The serum analysis showed that the concentration of different enzymes and lipid peroxidation products were significantly increased in exposed fish. It can be concluded that triazophos poses adverse hemato-biochemical and DNA damage effects in aquatic organisms.
The present study was conducted to explore the clinico-hematological and genotoxic effects in fre... more The present study was conducted to explore the clinico-hematological and
genotoxic effects in fresh water fish (Labeo rohita) exposed to various
concentrations of butachlor (chloroacetanilide herbicide). For this purpose 30 fish
weighing about 500-600 g were randomly allocated to five equal groups (A-E) and
treated with various concentrations (0, 0.25, 0.50, 0.75 and 1.00 mg/L) of butachlor.
Blood samples were collected from each fish after 48, 72 and 96 h of post treatment
and fresh thin blood smears were made for cellular abnormalities. All the fish kept
in group A did not reveal any clinical and behavioral ailments. However, various
clinical signs such as rapid breathing, gasping at the water surface, restlessness,
opercular movement, loss of equilibrium and erratic swimming were observed at
higher concentrations (0.75 and 1.00 mg/L) of herbicide. The results showed that
the erythrocyte, hemoglobin, hematocrit and lymphocyte values were significantly
(P<0.05) decreased while significantly increased total leukocyte counts were
recorded. Significantly increased incidence of different morphological and nuclear
abnormalities such as pear shape erythrocyte, microcyte, tear shape erythrocyte,
erythrocytes with micronuclei, lobed, blebbed and notched nuclei and cells with
nuclear remnants were observed. The results suggested that butachlor poses serious
clinico-hematological and mutagenic effects in fish and may be hazardous to the
other species when those are exposed to this herbicide.
The present experimental study was conducted to determine the clinico-hematological changes induc... more The present experimental study was conducted to determine the clinico-hematological changes induced by oral administration of triazophos in male Japanese quail. A total of 60 mature male quail were procured and randomly divided into five equal groups. All the experimental birds received triazophos orally @ 0, 2, 4, 6 and 8 mg/kg/day for 48 days. Birds were killed at day 16, 32 and 48 for collection of blood. Various clinical signs like ruffled feathers, tremors, watery droppings, salivation, torticollis, less foam production, decreased frequency of crowing and mating with their pen mates were observed at high doses (6 and 8 mg/kg/day triazophos). Significantly (P<0.05) lowered feed intake and body weight was recorded. Overall significantly (P<0.05) decreased total erythrocyte counts, total leukocyte counts, hemoglobin concentration, mean corpuscular hemoglobin concentration while increased pack cell volume and mean corpuscular volume was recorded in treated birds. It was concluded that triazophos induces clinico-hematological changes including anemia (macrocytic hypochromic) at higher doses in birds.