Francisco Cruz Sosa | Universidad Autónoma Metropolitana-Iztapalapa (original) (raw)
Papers by Francisco Cruz Sosa
In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 µM ... more In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 µM catechin, 25 µM vanillin, or 25 µM H 2 O 2. Of the treatments tested, 25 µM vanillin was the strongest inducer of haustoria in C. tenuiflora roots in vitro (up to 3 haustoria per root). Haustorium development occurred laterally and was observable 14 d after inducer application. It was characterized by elongation of the epidermal cells and division of the inner cortical cells which also possessed abundant nuclei. Histochemical analysis using 3,3-diaminobenzidine (DAB) and diphenylboric acid 2-aminoethyl ester (DBPA) indicated that the formation of haustoria was associated with the accumulation of H 2 O 2 and flavonoids.
Sphaeralcea angustifolia is used in Mexican traditional medicine to treat inflammatory processes.... more Sphaeralcea angustifolia is used in Mexican traditional medicine to treat inflammatory processes. SCopoletin (SC), TOmentin (TO), and sphaeralcic acid (SA) were reported as the main anti-inflammatory compounds in this species. The aim of this study was to establish in vitro conditions for the development of calli and cell suspension cultures that are the producers of these active compounds. Callus cultures of plant leaf explants were set up using different auxin levels of a-naphthalene acetic acid (NAA) in combination with a constant concentration (0.1 mg L-1) of Kinetin (Kn) in Murashige and Skoog (MS) medium. Optimal combinations for callus induction were 1.0 and 2.0 mg L-1 of NAA. SC, TO, and SA were not detected in callus tissues. Employing a 4 % inoculum in fresh biomass, cell suspension was established from friable callus with 1.0 mg L-1 of NAA in combination with 0.1 mg L-1 of Kn in MS liquid medium (27.4 mM nitrate). The cellular suspension synthesized SC and SA, SC was excreted into the culture medium, while SA was excreted into the culture medium and accumulated in biomass. To improve SC and SA production, total nitrate content was reduced in MS medium. On diminishing nitrate content to 2.74 mM, cellular suspension growth was not modified. SC concentration (0.04 %) was 60-fold higher than that detected in the wild plant (0.00067 %), TO was produced (0.096 %), and SA content (0.0036 %) was not improved. SA production in MS medium with 0.274 mM nitrate (0.004 %) was enriched 12-fold (0.0003 %) in relation to that of the wild plant. The anti-inflammatory effects at 5 h of intraperitoneal (i.p.) administration (100 mg per kg BW) of dichloromethane extracts from the medium (42 ± 3 %) and biomass (39 ± 9.3 %) of S. angustifolia cell suspensions cultivated in MS with 2.74 mM nitrate were similar. The effect of the biomass dichloromethane extract was dose dependent with a median Effective Dose (ED 50) of 137.63 mg per kg BW.
Buddleja cordata is a medicinal plant distributed in Mexican territory that is characterized for ... more Buddleja cordata is a medicinal plant distributed in Mexican territory that is characterized for producing phenolic
compounds possessing antioxidant activity. It was evaluated the type of morphogenetic responses induced by plant growth
regulators. Furthermore, the content of verbascoside and total phenols was determined, as well as the antioxidant activity
in regenerated plantlets. The greatest shoot proliferation (29.2 shoots per explant) was achieved in the stem-nodes that
were grown in a half-strength Murashige and Skoog medium containing 4.44 M N6-benzyladenine. The greatest shoot
heights of 4.3 and 4.7 cm (statistically not di erent) were obtained with gibberellic acid at 4.34 and 8.67 M, respectively.
The highest percentage of rooting (89.4%) occurred with 2.45-M indole-3-butyric acid with 20.3 roots per shoot and an
average root length of 4.4 cm. Of the rooted shoots, 91.7% were able to survive after 30 days of acclimatization. The
verbascoside (1.0 mg g1) and total phenols (24.8 mg of gallic acid equivalents g1) content was related to antioxidant
activity of the regenerated plantlets. The micropropagation of B. cordata might represent an alternative about massive
production and depict the basis of the establishment of commercial crops and genetic studies
Identification of candidate genes related to calanolide biosynthesis by transcriptome sequencing of Calophyllum brasiliense (Calophyllaceae)
Background: Calophyllum brasiliense is highlighted as an important resource of calanolides, which... more Background: Calophyllum brasiliense is highlighted as an important resource of calanolides, which are dipyranocoumarins that inhibit the reverse transcriptase of human immunodeficiency virus type 1 (HIV-1 RT). Despite having great medicinal importance, enzymes involved in calanolide, biosynthesis and the pathway itself, are still largely unknown. Additionally, no genomic resources exist for this plant species. Results: In this work, we first analyzed the transcriptome of C. brasiliense leaves, stem, and roots using a RNA-seq strategy, which provided a dataset for functional gene mining. According to the structures of the calanolides, putative biosynthetic pathways were proposed. Finally, candidate unigenes in the transcriptome dataset, potentially involved in umbelliferone and calanolide (angular pyranocoumarin) biosynthetic pathways, were screened using mainly homology-based BLAST and phylogenetic analyses.
Plant Physiology and Biochemistry, 2005
Prosopis laevigata nodal explants cultures were established in Murashige and Skoog medium. Simult... more Prosopis laevigata nodal explants cultures were established in Murashige and Skoog medium. Simultaneously these cultures were subjected to stress with biotic elicitors and an environmental factor (temperature increase to promote heat stress) in order to promote and increase exuded mesquite gum production. The biotic elicitors were: Aspergillus nidulans and Pseudomonas pseudoalcaligenes both used in concentrations of 10, 20 and 30 mg, whereas the environmental condition was different incubation temperatures (25, 35 and 40°C). The greatest gum production (~13 mg of pooled gum from 100 explants after 14 days incubation) took place when the culture medium was added 10, 20 and 30 mg of autoclaved fungal mycelium of A. nidulans or 30 mg of autoclaved bacterial biomass of P. pseudoalcaligenes in combination with an incubation temperature of 35°C. These treatments were non-significantly different among themselves (P < 0.05), but were significantly different to the rest of the treatments (P > 0.05).
Drying Technology, 2015
- Microencapsulation of chlorthalidone by spray-drying of double emulsion and melt granulatio... more 2016) Microencapsulation of chlorthalidone by spray-drying of double emulsion and melt granulation coating, Drying ABSTRACT Chlorthalidone (CH) is indicated in the management of hypertension either alone or in combination with other antihypertensive drugs, but it displays poor solubility and stability limiting its use in the development of new pharmaceutical alternatives. Spray-drying of double emulsions (SDE) and melt granulation coating (MGC) technologies were used for enhancing the stability of CH by using Eudragit 1 L30D-55 (EUD), Opadry 1 II (OPA), or Kollicoat 1 IR (KIR) as wall materials. SDE and MGC microcapsules containing CH were evaluated for particle size, morphology, release profiles, accelerated storage, and thermo-oxidative stability. Both types of microcapsules showed higher solubility (>80% in 20 min), lower degradation (<2%) under accelerated storage condition, and lower thermo-oxidative degradation than pure CH. However, SDE microcapsules showed smaller particle sizes (<16 µm) than MGC microcapsules (>700 µm), which widens the opportunity of incorporating microcapsules to dosage forms requiring different particle sizes for achieving their functionality.
Acta Physiologiae Plantarum, 2015
Acta Physiologiae Plantarum, 2013
Taxus globosa is the only species of the Taxus genus that grows in Mexico. In this study, callus ... more Taxus globosa is the only species of the Taxus genus that grows in Mexico. In this study, callus cultures from leaves and young shoots of T. globosa were established in Gamborg's B5 medium supplemented with 2,4dichlorophenoxiacetic acid (2 mg/L), kinetin (0.5 mg/L) and gibberellic acid (0.25 mg/L). Callus growth and taxane production were evaluated using two culture media: Woody Plant Medium and Gamborg's B5 supplemented with picloram (2 mg/L), kinetin (0.1 mg/L) and gibberellic acid (0.5 mg/L). The effect of the inoculum size (50, 100 and 150 g FW/L) and culture media (Woody Plant Medium and Gamborg's B5) with and without the presence of methyl jasmonate (100 lM) on T. globosa cell suspensions was assessed. Taxane analysis revealed that the calli in Gamborg's B5 produced taxol (50 lg/g DW), baccatin III, 10-deacetyl baccatin III and 10-deacetyl taxol. Woody Plant Medium also induced the production of taxol, although to a lesser extent. The optimum inoculum size was 50 g FW/L. In cell suspension cultures, both media had a significant effect on taxane production when supplemented with methyl jasmonate. In Woody Plant Medium, at day 14, a total concentration of 197.999 lg/L of taxol, 160.622 lg/L of baccatin III, 633.724 lg/L of 10-deacetyl baccatin III and 229.611 lg/L 10-deacetyl taxol were obtained, with total excretion of baccatin III and 10-deacetyl taxol to the culture medium. In Gamborg's B5, cephalomanine was obtained at a concentration of 91.428 lg/L without elicitation, and all taxanes were excreted to the medium to a variable extent.
Interciencia, Dec 1, 2008
Introducción. Los hidrocarburos aromáticos policíclicos (HAP) son un grupo de compuestos orgánico... more Introducción. Los hidrocarburos aromáticos policíclicos (HAP) son un grupo de compuestos orgánicos con dos o más anillos aromáticos. Los HAP se forman principalmente por la combustión incompleta de la materia orgánica, son compuestos potencialmente carcinogénicos o mutagénicos. En la actualidad se conocen técnicas biológicas como es el caso de la fitorremediación que tiene como objetivo degradar, asimilar, metabolizar o desintoxicar compuestos orgánicos aprovechando la acción combinada de plantas y sus microorganismos con capacidad fisiológica y bioquímica para absorber, retener, degradar o transformar sustancias contaminantes a formas menos tóxicas 1 . El objetivo de estudio fue evaluar la remoción de hidrocarburos por la interacción entre una planta (Festuca arundinacea) y un hongo filamentoso (Fusarium sp) ambos cultivados in vitro.
Revista mexicana de ingeniería química
Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo int... more Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)
Revista mexicana de ingeniería química
Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo int... more Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)
Polycyclic aromatic hydrocarbons (PAH) are widespread environmental pollutants of natural and ant... more Polycyclic aromatic hydrocarbons (PAH) are widespread environmental pollutants of natural and anthropogenic origins. Despite their poor water solubility, they can be taken up and bioaccumulated by plants. The effect of PAH on selected plant (Festuca arundinacea and Bouteloua curtipendula) at early grown stage was evaluated under in vitro conditions. Plant species were cultured on Murashige and Skoog (MS) medium with three different PAH: phenanthrene (PHE), pyrene (PYR), and benzo[a]pyrene (BaP). PAH plant accumulation and distribution were evaluated with GC and HPLC. To date, number phytoremediation mechanisms of pollutants removal are known, including: (i) degradation (phyto-and rhizodegradation), (ii) removal (phytoextraction, phytovolatilization) and (iii) immobilization (phytoestabilization). The objective of this study was to identify the phytoremediation mechanisms of selected plants use to control and/or remove PAH from MS medium. To accomplish the task, plant samples, after ...
Interciencia
A partir de explantes de embriones cigóticos y megagametofitos fueron inducidos cultivos organogé... more A partir de explantes de embriones cigóticos y megagametofitos fueron inducidos cultivos organogénicos de Dioon merolae del estado de Chiapas (México), cícada en peligro de extinción. El medio de inducción Litz consistió en los macronutrientes del medio B5, los micronutrientes del medio MS y los compuestos orgánicos glutamina (400mg·l-1) arginina (100mg·l-1), asparagina (100mg·l-1), sacarosa (60g·l-1), gellan-gum (4g), y suplementado con 0; 0,45; 2,26; 4,52 y 9,05μM αcido 2,4-diclorofenoxiacético (2,4-D), y 0; 2,32; 4,60; 9,30 y 13,90μM kinetina (K), con un arreglo de 5´5 con un diseño de bloque seleccionado al azar. Los cultivos fueron mantenidos en oscuridad a 25°C y el callo fue subcultivado en medio fresco cada cuatro semanas. La iniciación del callo ocurrió en un amplio rango de combinaciones de reguladores de crecimiento en explantes de megagametofito. El callo en explantes de embriones cigóticos se formó en pocas combinaciones. La formación de brotes adventicios ocurrió solo ...
Food Technology and Biotechnology
Revista mexicana de ingeniería química
A crude enzymatic extract from Aspergillus niger 10 with α-L-arabinofuranosidase activity (EC 3.2... more A crude enzymatic extract from Aspergillus niger 10 with α-L-arabinofuranosidase activity (EC 3.2.1.55) was obtained and its effect on the hydrolysis of mesquite gum was determined and compared to that of a commercial α-L-arabinofuranosidase from A. niger. The growth parameters of A. niger 10 obtained were X max = 3.03 g L -1 and μ max = 0.07 h -1 . The maximum enzymatic activity obtained was 65.93 U L -1 . Optimum temperature and activation energy for the crude extract were 50°C and 46.15 KJ mol -1 and for the commercial enzyme 40 °C and 52.76 KJ mol -1 , respectively. The apparent kinetic parameters K m and V max for the crude extract were 4.87 g L -1 and 0.15 μmol min -1 g -1 , and for the commercial enzyme 76.45 g L -1 and 3.85 μmol min -1 g -1 , respectively. Yields of Larabinose recovery for the crude extract and the commercial enzyme were 17.04 % and 2.78 %, respectively, based on the reported average content of L-arabinose in mesquite gum.
In this work the importance of soluble dietary fibers in the human diet is discussed. Traditional... more In this work the importance of soluble dietary fibers in the human diet is discussed. Traditional and new sources of soluble dietary fiber are mentioned, and a description of how to apply them in different types of beverages such as energy drinks, sport drinks, carbonated beverages and protein-based beverages in order to achieve enhanced functional properties is given.
Introducción. El cultivo de tejidos vegetales representa una fuente potencial y sustentable de co... more Introducción. El cultivo de tejidos vegetales representa una fuente potencial y sustentable de compuestos medicinales, sabores, fragancias y colorantes, que actualmente no son producidos por microorganismos o bien por síntesis química (2). La lavanda (Lavandula angustifolia) es una planta que crece en clima templado, posee follaje abundante y flores color púrpura que desprenden una agradable fragancia además de producir un pigmento del mismo color. El extracto de lavanda que puede ser el pigmento o el aceite, es utilizado en productos de perfumería, por sus características relajantes y decorativas. Actualmente el aceite puede ser de origen natural o artificial, siendo el primero el más buscado entre los fabricantes de productos cosméticos para evitar posibles reacciones secundarias que afecten la salud de los consumidores. Para cubrir la demanda de aceite esencial se han desarrollado métodos de extracción utilizando diversas combinaciones de solventes que incrementen su rendimiento, siendo la materia prima el follaje y en menor proporción las flores secas o frescas.
In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 µM ... more In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 µM catechin, 25 µM vanillin, or 25 µM H 2 O 2. Of the treatments tested, 25 µM vanillin was the strongest inducer of haustoria in C. tenuiflora roots in vitro (up to 3 haustoria per root). Haustorium development occurred laterally and was observable 14 d after inducer application. It was characterized by elongation of the epidermal cells and division of the inner cortical cells which also possessed abundant nuclei. Histochemical analysis using 3,3-diaminobenzidine (DAB) and diphenylboric acid 2-aminoethyl ester (DBPA) indicated that the formation of haustoria was associated with the accumulation of H 2 O 2 and flavonoids.
Sphaeralcea angustifolia is used in Mexican traditional medicine to treat inflammatory processes.... more Sphaeralcea angustifolia is used in Mexican traditional medicine to treat inflammatory processes. SCopoletin (SC), TOmentin (TO), and sphaeralcic acid (SA) were reported as the main anti-inflammatory compounds in this species. The aim of this study was to establish in vitro conditions for the development of calli and cell suspension cultures that are the producers of these active compounds. Callus cultures of plant leaf explants were set up using different auxin levels of a-naphthalene acetic acid (NAA) in combination with a constant concentration (0.1 mg L-1) of Kinetin (Kn) in Murashige and Skoog (MS) medium. Optimal combinations for callus induction were 1.0 and 2.0 mg L-1 of NAA. SC, TO, and SA were not detected in callus tissues. Employing a 4 % inoculum in fresh biomass, cell suspension was established from friable callus with 1.0 mg L-1 of NAA in combination with 0.1 mg L-1 of Kn in MS liquid medium (27.4 mM nitrate). The cellular suspension synthesized SC and SA, SC was excreted into the culture medium, while SA was excreted into the culture medium and accumulated in biomass. To improve SC and SA production, total nitrate content was reduced in MS medium. On diminishing nitrate content to 2.74 mM, cellular suspension growth was not modified. SC concentration (0.04 %) was 60-fold higher than that detected in the wild plant (0.00067 %), TO was produced (0.096 %), and SA content (0.0036 %) was not improved. SA production in MS medium with 0.274 mM nitrate (0.004 %) was enriched 12-fold (0.0003 %) in relation to that of the wild plant. The anti-inflammatory effects at 5 h of intraperitoneal (i.p.) administration (100 mg per kg BW) of dichloromethane extracts from the medium (42 ± 3 %) and biomass (39 ± 9.3 %) of S. angustifolia cell suspensions cultivated in MS with 2.74 mM nitrate were similar. The effect of the biomass dichloromethane extract was dose dependent with a median Effective Dose (ED 50) of 137.63 mg per kg BW.
Buddleja cordata is a medicinal plant distributed in Mexican territory that is characterized for ... more Buddleja cordata is a medicinal plant distributed in Mexican territory that is characterized for producing phenolic
compounds possessing antioxidant activity. It was evaluated the type of morphogenetic responses induced by plant growth
regulators. Furthermore, the content of verbascoside and total phenols was determined, as well as the antioxidant activity
in regenerated plantlets. The greatest shoot proliferation (29.2 shoots per explant) was achieved in the stem-nodes that
were grown in a half-strength Murashige and Skoog medium containing 4.44 M N6-benzyladenine. The greatest shoot
heights of 4.3 and 4.7 cm (statistically not di erent) were obtained with gibberellic acid at 4.34 and 8.67 M, respectively.
The highest percentage of rooting (89.4%) occurred with 2.45-M indole-3-butyric acid with 20.3 roots per shoot and an
average root length of 4.4 cm. Of the rooted shoots, 91.7% were able to survive after 30 days of acclimatization. The
verbascoside (1.0 mg g1) and total phenols (24.8 mg of gallic acid equivalents g1) content was related to antioxidant
activity of the regenerated plantlets. The micropropagation of B. cordata might represent an alternative about massive
production and depict the basis of the establishment of commercial crops and genetic studies
Identification of candidate genes related to calanolide biosynthesis by transcriptome sequencing of Calophyllum brasiliense (Calophyllaceae)
Background: Calophyllum brasiliense is highlighted as an important resource of calanolides, which... more Background: Calophyllum brasiliense is highlighted as an important resource of calanolides, which are dipyranocoumarins that inhibit the reverse transcriptase of human immunodeficiency virus type 1 (HIV-1 RT). Despite having great medicinal importance, enzymes involved in calanolide, biosynthesis and the pathway itself, are still largely unknown. Additionally, no genomic resources exist for this plant species. Results: In this work, we first analyzed the transcriptome of C. brasiliense leaves, stem, and roots using a RNA-seq strategy, which provided a dataset for functional gene mining. According to the structures of the calanolides, putative biosynthetic pathways were proposed. Finally, candidate unigenes in the transcriptome dataset, potentially involved in umbelliferone and calanolide (angular pyranocoumarin) biosynthetic pathways, were screened using mainly homology-based BLAST and phylogenetic analyses.
Plant Physiology and Biochemistry, 2005
Prosopis laevigata nodal explants cultures were established in Murashige and Skoog medium. Simult... more Prosopis laevigata nodal explants cultures were established in Murashige and Skoog medium. Simultaneously these cultures were subjected to stress with biotic elicitors and an environmental factor (temperature increase to promote heat stress) in order to promote and increase exuded mesquite gum production. The biotic elicitors were: Aspergillus nidulans and Pseudomonas pseudoalcaligenes both used in concentrations of 10, 20 and 30 mg, whereas the environmental condition was different incubation temperatures (25, 35 and 40°C). The greatest gum production (~13 mg of pooled gum from 100 explants after 14 days incubation) took place when the culture medium was added 10, 20 and 30 mg of autoclaved fungal mycelium of A. nidulans or 30 mg of autoclaved bacterial biomass of P. pseudoalcaligenes in combination with an incubation temperature of 35°C. These treatments were non-significantly different among themselves (P < 0.05), but were significantly different to the rest of the treatments (P > 0.05).
Drying Technology, 2015
- Microencapsulation of chlorthalidone by spray-drying of double emulsion and melt granulatio... more 2016) Microencapsulation of chlorthalidone by spray-drying of double emulsion and melt granulation coating, Drying ABSTRACT Chlorthalidone (CH) is indicated in the management of hypertension either alone or in combination with other antihypertensive drugs, but it displays poor solubility and stability limiting its use in the development of new pharmaceutical alternatives. Spray-drying of double emulsions (SDE) and melt granulation coating (MGC) technologies were used for enhancing the stability of CH by using Eudragit 1 L30D-55 (EUD), Opadry 1 II (OPA), or Kollicoat 1 IR (KIR) as wall materials. SDE and MGC microcapsules containing CH were evaluated for particle size, morphology, release profiles, accelerated storage, and thermo-oxidative stability. Both types of microcapsules showed higher solubility (>80% in 20 min), lower degradation (<2%) under accelerated storage condition, and lower thermo-oxidative degradation than pure CH. However, SDE microcapsules showed smaller particle sizes (<16 µm) than MGC microcapsules (>700 µm), which widens the opportunity of incorporating microcapsules to dosage forms requiring different particle sizes for achieving their functionality.
Acta Physiologiae Plantarum, 2015
Acta Physiologiae Plantarum, 2013
Taxus globosa is the only species of the Taxus genus that grows in Mexico. In this study, callus ... more Taxus globosa is the only species of the Taxus genus that grows in Mexico. In this study, callus cultures from leaves and young shoots of T. globosa were established in Gamborg's B5 medium supplemented with 2,4dichlorophenoxiacetic acid (2 mg/L), kinetin (0.5 mg/L) and gibberellic acid (0.25 mg/L). Callus growth and taxane production were evaluated using two culture media: Woody Plant Medium and Gamborg's B5 supplemented with picloram (2 mg/L), kinetin (0.1 mg/L) and gibberellic acid (0.5 mg/L). The effect of the inoculum size (50, 100 and 150 g FW/L) and culture media (Woody Plant Medium and Gamborg's B5) with and without the presence of methyl jasmonate (100 lM) on T. globosa cell suspensions was assessed. Taxane analysis revealed that the calli in Gamborg's B5 produced taxol (50 lg/g DW), baccatin III, 10-deacetyl baccatin III and 10-deacetyl taxol. Woody Plant Medium also induced the production of taxol, although to a lesser extent. The optimum inoculum size was 50 g FW/L. In cell suspension cultures, both media had a significant effect on taxane production when supplemented with methyl jasmonate. In Woody Plant Medium, at day 14, a total concentration of 197.999 lg/L of taxol, 160.622 lg/L of baccatin III, 633.724 lg/L of 10-deacetyl baccatin III and 229.611 lg/L 10-deacetyl taxol were obtained, with total excretion of baccatin III and 10-deacetyl taxol to the culture medium. In Gamborg's B5, cephalomanine was obtained at a concentration of 91.428 lg/L without elicitation, and all taxanes were excreted to the medium to a variable extent.
Interciencia, Dec 1, 2008
Introducción. Los hidrocarburos aromáticos policíclicos (HAP) son un grupo de compuestos orgánico... more Introducción. Los hidrocarburos aromáticos policíclicos (HAP) son un grupo de compuestos orgánicos con dos o más anillos aromáticos. Los HAP se forman principalmente por la combustión incompleta de la materia orgánica, son compuestos potencialmente carcinogénicos o mutagénicos. En la actualidad se conocen técnicas biológicas como es el caso de la fitorremediación que tiene como objetivo degradar, asimilar, metabolizar o desintoxicar compuestos orgánicos aprovechando la acción combinada de plantas y sus microorganismos con capacidad fisiológica y bioquímica para absorber, retener, degradar o transformar sustancias contaminantes a formas menos tóxicas 1 . El objetivo de estudio fue evaluar la remoción de hidrocarburos por la interacción entre una planta (Festuca arundinacea) y un hongo filamentoso (Fusarium sp) ambos cultivados in vitro.
Revista mexicana de ingeniería química
Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo int... more Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)
Revista mexicana de ingeniería química
Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo int... more Modelado de la biodegradación en biorreactores de lodos de hidrocarburos totales del petróleo intemperizados en suelos y sedimentos (Biodegradation modeling of sludge bioreactors of total petroleum hydrocarbons weathering in soil and sediments)
Polycyclic aromatic hydrocarbons (PAH) are widespread environmental pollutants of natural and ant... more Polycyclic aromatic hydrocarbons (PAH) are widespread environmental pollutants of natural and anthropogenic origins. Despite their poor water solubility, they can be taken up and bioaccumulated by plants. The effect of PAH on selected plant (Festuca arundinacea and Bouteloua curtipendula) at early grown stage was evaluated under in vitro conditions. Plant species were cultured on Murashige and Skoog (MS) medium with three different PAH: phenanthrene (PHE), pyrene (PYR), and benzo[a]pyrene (BaP). PAH plant accumulation and distribution were evaluated with GC and HPLC. To date, number phytoremediation mechanisms of pollutants removal are known, including: (i) degradation (phyto-and rhizodegradation), (ii) removal (phytoextraction, phytovolatilization) and (iii) immobilization (phytoestabilization). The objective of this study was to identify the phytoremediation mechanisms of selected plants use to control and/or remove PAH from MS medium. To accomplish the task, plant samples, after ...
Interciencia
A partir de explantes de embriones cigóticos y megagametofitos fueron inducidos cultivos organogé... more A partir de explantes de embriones cigóticos y megagametofitos fueron inducidos cultivos organogénicos de Dioon merolae del estado de Chiapas (México), cícada en peligro de extinción. El medio de inducción Litz consistió en los macronutrientes del medio B5, los micronutrientes del medio MS y los compuestos orgánicos glutamina (400mg·l-1) arginina (100mg·l-1), asparagina (100mg·l-1), sacarosa (60g·l-1), gellan-gum (4g), y suplementado con 0; 0,45; 2,26; 4,52 y 9,05μM αcido 2,4-diclorofenoxiacético (2,4-D), y 0; 2,32; 4,60; 9,30 y 13,90μM kinetina (K), con un arreglo de 5´5 con un diseño de bloque seleccionado al azar. Los cultivos fueron mantenidos en oscuridad a 25°C y el callo fue subcultivado en medio fresco cada cuatro semanas. La iniciación del callo ocurrió en un amplio rango de combinaciones de reguladores de crecimiento en explantes de megagametofito. El callo en explantes de embriones cigóticos se formó en pocas combinaciones. La formación de brotes adventicios ocurrió solo ...
Food Technology and Biotechnology
Revista mexicana de ingeniería química
A crude enzymatic extract from Aspergillus niger 10 with α-L-arabinofuranosidase activity (EC 3.2... more A crude enzymatic extract from Aspergillus niger 10 with α-L-arabinofuranosidase activity (EC 3.2.1.55) was obtained and its effect on the hydrolysis of mesquite gum was determined and compared to that of a commercial α-L-arabinofuranosidase from A. niger. The growth parameters of A. niger 10 obtained were X max = 3.03 g L -1 and μ max = 0.07 h -1 . The maximum enzymatic activity obtained was 65.93 U L -1 . Optimum temperature and activation energy for the crude extract were 50°C and 46.15 KJ mol -1 and for the commercial enzyme 40 °C and 52.76 KJ mol -1 , respectively. The apparent kinetic parameters K m and V max for the crude extract were 4.87 g L -1 and 0.15 μmol min -1 g -1 , and for the commercial enzyme 76.45 g L -1 and 3.85 μmol min -1 g -1 , respectively. Yields of Larabinose recovery for the crude extract and the commercial enzyme were 17.04 % and 2.78 %, respectively, based on the reported average content of L-arabinose in mesquite gum.
In this work the importance of soluble dietary fibers in the human diet is discussed. Traditional... more In this work the importance of soluble dietary fibers in the human diet is discussed. Traditional and new sources of soluble dietary fiber are mentioned, and a description of how to apply them in different types of beverages such as energy drinks, sport drinks, carbonated beverages and protein-based beverages in order to achieve enhanced functional properties is given.
Introducción. El cultivo de tejidos vegetales representa una fuente potencial y sustentable de co... more Introducción. El cultivo de tejidos vegetales representa una fuente potencial y sustentable de compuestos medicinales, sabores, fragancias y colorantes, que actualmente no son producidos por microorganismos o bien por síntesis química (2). La lavanda (Lavandula angustifolia) es una planta que crece en clima templado, posee follaje abundante y flores color púrpura que desprenden una agradable fragancia además de producir un pigmento del mismo color. El extracto de lavanda que puede ser el pigmento o el aceite, es utilizado en productos de perfumería, por sus características relajantes y decorativas. Actualmente el aceite puede ser de origen natural o artificial, siendo el primero el más buscado entre los fabricantes de productos cosméticos para evitar posibles reacciones secundarias que afecten la salud de los consumidores. Para cubrir la demanda de aceite esencial se han desarrollado métodos de extracción utilizando diversas combinaciones de solventes que incrementen su rendimiento, siendo la materia prima el follaje y en menor proporción las flores secas o frescas.