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Research paper thumbnail of Piriformospora indica rescues growth diminution of rice seedlings during high salt stress

Plant Signaling & Behavior, 2013

Research paper thumbnail of Insights into the Functions of eIF4E-Binding Motif of VPg in Potato Virus A Infection

Viruses

The interaction between the viral protein genome-linked (VPg) and eukaryotic initiation factor 4E... more The interaction between the viral protein genome-linked (VPg) and eukaryotic initiation factor 4E (eIF4E) or eIF(iso)4E of the host plays a crucial role in potyvirus infection. The VPg of potato virus A (PVA) contains the Tyr-X-X-X-X-Leu-phi (YXXXLΦ) binding motif for eIF(iso)4E. In order to investigate its role in PVA infection, we substituted the conserved tyrosine and leucine residues of the motif with alanine residues in the infectious cDNA of PVA (PVAVPgmut). PVAVPgmut RNA replicated in infiltrated leaves, but RNA accumulation remained low. Systemic infection occurred only if a reversion to wild type PVA occurred. VPg was able to stabilize PVA RNA and enhance the expression of Renilla luciferase (3’RLUC) from the 3’ end of the PVA genome. VPgmut could not support either PVA RNA stabilization or enhanced 3’RLUC expression. The RNA silencing suppressor helper-component proteinase (HCPro) is responsible for the formation of PVA-induced RNA granules (PGs) during infection. While VP...

Research paper thumbnail of Dynamics of protein accumulation from the 3′end of viral RNA is different from the rest of the genome in potato virus A infection

Journal of Virology

One large open reading frame (ORF) encodes for ten potyviral proteins. We compared accumulation o... more One large open reading frame (ORF) encodes for ten potyviral proteins. We compared accumulation of cylindrical inclusion protein (CI) from the middle, coat protein (CP) from the 3′end and Renilla luciferase (RLUC) from two distinct locations in potato virus A (PVA) RNA. 5′RLUC was expressed from an rluc gene inserted between P1 and helper component proteinase (HCPro) cistrons and 3′RLUC from the gene inserted between RNA polymerase and CP cistrons. Viral protein and RNA accumulation was quantitated 1) when expressed from PVA RNA in the presence of ectopically expressed viral protein genome-linked (VPg) and auxiliary proteins and 2) at different time points during natural infection. The rate and timing of 3′RLUC and CP accumulation were found to be different from those of 5′RLUC and CI. Ectopic expression of VPg boosted PVA RNA, 3′RLUC and, together with HCPro, CP accumulation whereas 5′RLUC and CI accumulation remained unaffected regardless of the increased viral RNA amount. In natu...

Research paper thumbnail of Piriformospora indica rescues growth diminution of rice seedlings during high salt stress

Plant Signaling & Behavior, 2013

Research paper thumbnail of Insights into the Functions of eIF4E-Binding Motif of VPg in Potato Virus A Infection

Viruses

The interaction between the viral protein genome-linked (VPg) and eukaryotic initiation factor 4E... more The interaction between the viral protein genome-linked (VPg) and eukaryotic initiation factor 4E (eIF4E) or eIF(iso)4E of the host plays a crucial role in potyvirus infection. The VPg of potato virus A (PVA) contains the Tyr-X-X-X-X-Leu-phi (YXXXLΦ) binding motif for eIF(iso)4E. In order to investigate its role in PVA infection, we substituted the conserved tyrosine and leucine residues of the motif with alanine residues in the infectious cDNA of PVA (PVAVPgmut). PVAVPgmut RNA replicated in infiltrated leaves, but RNA accumulation remained low. Systemic infection occurred only if a reversion to wild type PVA occurred. VPg was able to stabilize PVA RNA and enhance the expression of Renilla luciferase (3’RLUC) from the 3’ end of the PVA genome. VPgmut could not support either PVA RNA stabilization or enhanced 3’RLUC expression. The RNA silencing suppressor helper-component proteinase (HCPro) is responsible for the formation of PVA-induced RNA granules (PGs) during infection. While VP...

Research paper thumbnail of Dynamics of protein accumulation from the 3′end of viral RNA is different from the rest of the genome in potato virus A infection

Journal of Virology

One large open reading frame (ORF) encodes for ten potyviral proteins. We compared accumulation o... more One large open reading frame (ORF) encodes for ten potyviral proteins. We compared accumulation of cylindrical inclusion protein (CI) from the middle, coat protein (CP) from the 3′end and Renilla luciferase (RLUC) from two distinct locations in potato virus A (PVA) RNA. 5′RLUC was expressed from an rluc gene inserted between P1 and helper component proteinase (HCPro) cistrons and 3′RLUC from the gene inserted between RNA polymerase and CP cistrons. Viral protein and RNA accumulation was quantitated 1) when expressed from PVA RNA in the presence of ectopically expressed viral protein genome-linked (VPg) and auxiliary proteins and 2) at different time points during natural infection. The rate and timing of 3′RLUC and CP accumulation were found to be different from those of 5′RLUC and CI. Ectopic expression of VPg boosted PVA RNA, 3′RLUC and, together with HCPro, CP accumulation whereas 5′RLUC and CI accumulation remained unaffected regardless of the increased viral RNA amount. In natu...

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