Ahmed Ismail | Jazan University (original) (raw)

Papers by Ahmed Ismail

Research paper thumbnail of Progressing nanotechnology to improve targeted cancer treatment: overcoming hurdles in its clinical implementation

Molecular Cancer, Oct 8, 2023

The use of nanotechnology has the potential to revolutionize the detection and treatment of cance... more The use of nanotechnology has the potential to revolutionize the detection and treatment of cancer. Developments in protein engineering and materials science have led to the emergence of new nanoscale targeting techniques, which offer renewed hope for cancer patients. While several nanocarriers for medicinal purposes have been approved for human trials, only a few have been authorized for clinical use in targeting cancer cells. In this review, we analyze some of the authorized formulations and discuss the challenges of translating findings from the lab to the clinic. This study highlights the various nanocarriers and compounds that can be used for selective tumor targeting and the inherent difficulties in cancer therapy. Nanotechnology provides a promising platform for improving cancer detection and treatment in the future, but further research is needed to overcome the current limitations in clinical translation.

Research paper thumbnail of Publisher Correction to: Evaluation of the immunomodulatory, antioxidant, and histopathological effects of Cymbopogon schoenanthus essential oil extract on kidney and spleen in BALB/C mice

Journal of Umm Al-Qura University for Applied Sciences

In this article, the order of references was incorrect. The reference list has been updated as sh... more In this article, the order of references was incorrect. The reference list has been updated as shown below. Spelling of co-author "K. M. El-Azab" updated to "Khalid M. Elazab". The original article has been corrected.

Research paper thumbnail of The role of bee venom on immunological and hematological parameters in albino rats

Egyptian journal of Immunology

Bee venom (BV) showed therapeutical effects to treat various diseases as it contains at least 18 ... more Bee venom (BV) showed therapeutical effects to treat various diseases as it contains at least 18 pharmacologically active components including various enzymes, peptides, and amines. This study aimed to evaluate the action of BV on some hematological parameters, humoral and cellular immunity, and the determination of antioxidant levels in male albino rats. The study included 40 male albino rats (190–210 g), divided into four groups. Three groups were injected subcutaneously with three different doses of BV (2.5, 5, and 10 mg/kg, respectively). The control group was injected with saline solution. Blood samples were obtained to measure total leucocytes count (TLC), differential leukocytes count, hematological parameters (hemoglobin (Hb), hematocrit (HCT), red blood cells (RBCs), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC) and Platelets. Sera were used to assess immunoglobulins (IgM, IgG, IgA, and IgE), some cytokines e.g., tumor necrosi...

Research paper thumbnail of Immunomodulatory effect of curcumin on hepatic cirrhosis in experimental rats

Journal of Food Biochemistry, 2020

Liver diseases are considered as an important diagnostic sign for body health. In such countries ... more Liver diseases are considered as an important diagnostic sign for body health. In such countries that suffers from obesity and alcohol abuse; liver diseases obviously occur, while in poor localities, the main cause of liver diseases are infectious diseases mainly from viruses and parasites as in Africa and Asia (Buonomo et al., 2019). Liver fibrosis occurs as a result of chronic liver diseases, in which excessive accumulation of some extracellular proteins such as collagen are presented. Information about the cellular and molecular mechanisms of liver fibrosis has been

Research paper thumbnail of Evaluation of the immunomodulatory, antioxidant, and histopathological effects of Cymbopogon schoenanthus essential oil extract on kidney and spleen in BALB/C Mice

Journal of Umm Al-Qura University for Applied Sciences

This study aimed to evaluate the impact of C. schoenanthus essential oil (CSEO) on the immune sys... more This study aimed to evaluate the impact of C. schoenanthus essential oil (CSEO) on the immune system, antioxidant balance, and histological changes in the kidney and spleen of female BALB/C mice. The chemical composition of CSEO was analyzed using GC-MS. Twenty-nine compounds were identified, representing 99.04% of the total detected. The main components were Piperitone (47.93%), Elemol (11.91%), 2-Carene (10.69%), β-eudesmol (7.67%), α-eudesmol (5.12%), and γ-eudesmol (4.24%). For bioassay, the mice were divided into three groups: control, 0.5% CSEO (G1), and 1% CSEO (G2). The effects of CSEO on various markers, including malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), urea, creatinine, and cytokines (IL-4, TNF-α, and TGF-β1), were evaluated. Additionally, kidney and spleen histopathological examinations were conducted. The treatment groups showed a significant increase in IL-4, TNF-α, and TGF-β1 levels compared to the control group, except for...

Research paper thumbnail of Standpoints on immune responses against COVID-19

Journal of Bioscience and Applied Research, 2020

BioBacta

Research paper thumbnail of Ameliorative effects of melatonin against solid Ehrlich carcinoma progression in female mice

Journal of Pineal Research, 2019

The current work estimated the antitumour efficacy of melatonin (MLT) on the growth of Ehrlich as... more The current work estimated the antitumour efficacy of melatonin (MLT) on the growth of Ehrlich ascites carcinoma cells inoculated intramuscularly into the hind limbs of female BALB/c mice and to compare its effects with those of adriamycin (ADR). After solid tumours developed, the animals were divided into the three following groups: the tumour‐bearing control, MLT‐treated (20 mg/kg body weight) and ADR‐treated (10 mg/kg body weight) groups. The results showed a significant reduction in the tumour masses of the treated animals in comparison with those of the control group. There were a significant decrease in the malondialdehyde level and a significant elevation of the glutathione concentration and the superoxide dismutase and catalase activities in the MLT and ADR groups. The current study indicated the increased expression levels of P53, caspase‐3 and caspase‐9 and the decreased expression levels of the rRNA and Bcl2. The MLT and ADR treatments resulted in histological changes, such as a marked degenerative area, the necrosis of neoplastic cells, the appearance of different forms of apoptotic cells and giant cells with condensed chromatin, and a deeply eosinophilic cytoplasm. The MLT and ADR treatments also significantly decreased the Ki‐67 protein and vascular endothelial growth factor (VEGF) expression levels in the tumour masses. In conclusion, similar to ADR‐treated tumour‐bearing mice, MLT suppressed the growth and proliferation of tumour by inducing apoptosis and by inhibiting tumour vascularization. The current data recommend MLT as a safe natural chemotherapeutic adjuvant to overcome cancer progression after a clinical trial validates these results.

Research paper thumbnail of Immunomodulatory effect of papaya ( Carica papaya ) pulp and seed extracts as a potential natural treatment for bacterial stress

Journal of Food Biochemistry, 2019

The current study evaluated the immunomodulatory effects of Carica papaya pulp and seeds methanol... more The current study evaluated the immunomodulatory effects of Carica papaya pulp and seeds methanol (MeOH) extracts on mice infected with Listeria monocytogenes. Gas chromatography-mass spectrometry analysis identified 10 active constituents in C. papaya seed MeOH extract and 10 compounds in C. papaya pulp MeOH extract. The experimental animals were divided into negative control (G1) group, positive control (G2) group, pulp extract treated (G3) group, and seed extract treated (G4) group. After infection of animals (G2, G3, and G4), treatments were started for 3 weeks. Estimation of the immunological parameters showed a marked decrease in IgM levels and an increase in IgG levels in the treated groups (G3 and G4) compared with those in G2. The proinflammatory cytokines (IL-10, IL-12, IL-1β, IL-6, and TGF-β1) were decreased in the treated groups (G3 and G4) compared with those in G2. Nitric oxide levels were also decreased, and the percentages of phagocytosis increased compared with those of G2. The results demonstrated the immunomodulatory and anti-inflammatory effects of C. papaya pulp and seeds MeOH extracts. PRACTICAL APPLICATIONS: Based on the antioxidant and antibacterial activities exhibited by the pulp and seed MeOH extracts investigated in this study, Carica papaya might be considered as a natural source of phytochemicals that could be utilized in novel foods and pharmaceuticals. Further investigation are needed to identify and purify compounds that might be responsible for the observed effects.

Research paper thumbnail of Progressing nanotechnology to improve targeted cancer treatment: overcoming hurdles in its clinical implementation

Molecular Cancer, Oct 8, 2023

The use of nanotechnology has the potential to revolutionize the detection and treatment of cance... more The use of nanotechnology has the potential to revolutionize the detection and treatment of cancer. Developments in protein engineering and materials science have led to the emergence of new nanoscale targeting techniques, which offer renewed hope for cancer patients. While several nanocarriers for medicinal purposes have been approved for human trials, only a few have been authorized for clinical use in targeting cancer cells. In this review, we analyze some of the authorized formulations and discuss the challenges of translating findings from the lab to the clinic. This study highlights the various nanocarriers and compounds that can be used for selective tumor targeting and the inherent difficulties in cancer therapy. Nanotechnology provides a promising platform for improving cancer detection and treatment in the future, but further research is needed to overcome the current limitations in clinical translation.

Research paper thumbnail of Publisher Correction to: Evaluation of the immunomodulatory, antioxidant, and histopathological effects of Cymbopogon schoenanthus essential oil extract on kidney and spleen in BALB/C mice

Journal of Umm Al-Qura University for Applied Sciences

In this article, the order of references was incorrect. The reference list has been updated as sh... more In this article, the order of references was incorrect. The reference list has been updated as shown below. Spelling of co-author "K. M. El-Azab" updated to "Khalid M. Elazab". The original article has been corrected.

Research paper thumbnail of The role of bee venom on immunological and hematological parameters in albino rats

Egyptian journal of Immunology

Bee venom (BV) showed therapeutical effects to treat various diseases as it contains at least 18 ... more Bee venom (BV) showed therapeutical effects to treat various diseases as it contains at least 18 pharmacologically active components including various enzymes, peptides, and amines. This study aimed to evaluate the action of BV on some hematological parameters, humoral and cellular immunity, and the determination of antioxidant levels in male albino rats. The study included 40 male albino rats (190–210 g), divided into four groups. Three groups were injected subcutaneously with three different doses of BV (2.5, 5, and 10 mg/kg, respectively). The control group was injected with saline solution. Blood samples were obtained to measure total leucocytes count (TLC), differential leukocytes count, hematological parameters (hemoglobin (Hb), hematocrit (HCT), red blood cells (RBCs), mean cell volume (MCV), mean cell hemoglobin (MCH), mean cell hemoglobin concentration (MCHC) and Platelets. Sera were used to assess immunoglobulins (IgM, IgG, IgA, and IgE), some cytokines e.g., tumor necrosi...

Research paper thumbnail of Immunomodulatory effect of curcumin on hepatic cirrhosis in experimental rats

Journal of Food Biochemistry, 2020

Liver diseases are considered as an important diagnostic sign for body health. In such countries ... more Liver diseases are considered as an important diagnostic sign for body health. In such countries that suffers from obesity and alcohol abuse; liver diseases obviously occur, while in poor localities, the main cause of liver diseases are infectious diseases mainly from viruses and parasites as in Africa and Asia (Buonomo et al., 2019). Liver fibrosis occurs as a result of chronic liver diseases, in which excessive accumulation of some extracellular proteins such as collagen are presented. Information about the cellular and molecular mechanisms of liver fibrosis has been

Research paper thumbnail of Evaluation of the immunomodulatory, antioxidant, and histopathological effects of Cymbopogon schoenanthus essential oil extract on kidney and spleen in BALB/C Mice

Journal of Umm Al-Qura University for Applied Sciences

This study aimed to evaluate the impact of C. schoenanthus essential oil (CSEO) on the immune sys... more This study aimed to evaluate the impact of C. schoenanthus essential oil (CSEO) on the immune system, antioxidant balance, and histological changes in the kidney and spleen of female BALB/C mice. The chemical composition of CSEO was analyzed using GC-MS. Twenty-nine compounds were identified, representing 99.04% of the total detected. The main components were Piperitone (47.93%), Elemol (11.91%), 2-Carene (10.69%), β-eudesmol (7.67%), α-eudesmol (5.12%), and γ-eudesmol (4.24%). For bioassay, the mice were divided into three groups: control, 0.5% CSEO (G1), and 1% CSEO (G2). The effects of CSEO on various markers, including malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), urea, creatinine, and cytokines (IL-4, TNF-α, and TGF-β1), were evaluated. Additionally, kidney and spleen histopathological examinations were conducted. The treatment groups showed a significant increase in IL-4, TNF-α, and TGF-β1 levels compared to the control group, except for...

Research paper thumbnail of Standpoints on immune responses against COVID-19

Journal of Bioscience and Applied Research, 2020

BioBacta

Research paper thumbnail of Ameliorative effects of melatonin against solid Ehrlich carcinoma progression in female mice

Journal of Pineal Research, 2019

The current work estimated the antitumour efficacy of melatonin (MLT) on the growth of Ehrlich as... more The current work estimated the antitumour efficacy of melatonin (MLT) on the growth of Ehrlich ascites carcinoma cells inoculated intramuscularly into the hind limbs of female BALB/c mice and to compare its effects with those of adriamycin (ADR). After solid tumours developed, the animals were divided into the three following groups: the tumour‐bearing control, MLT‐treated (20 mg/kg body weight) and ADR‐treated (10 mg/kg body weight) groups. The results showed a significant reduction in the tumour masses of the treated animals in comparison with those of the control group. There were a significant decrease in the malondialdehyde level and a significant elevation of the glutathione concentration and the superoxide dismutase and catalase activities in the MLT and ADR groups. The current study indicated the increased expression levels of P53, caspase‐3 and caspase‐9 and the decreased expression levels of the rRNA and Bcl2. The MLT and ADR treatments resulted in histological changes, such as a marked degenerative area, the necrosis of neoplastic cells, the appearance of different forms of apoptotic cells and giant cells with condensed chromatin, and a deeply eosinophilic cytoplasm. The MLT and ADR treatments also significantly decreased the Ki‐67 protein and vascular endothelial growth factor (VEGF) expression levels in the tumour masses. In conclusion, similar to ADR‐treated tumour‐bearing mice, MLT suppressed the growth and proliferation of tumour by inducing apoptosis and by inhibiting tumour vascularization. The current data recommend MLT as a safe natural chemotherapeutic adjuvant to overcome cancer progression after a clinical trial validates these results.

Research paper thumbnail of Immunomodulatory effect of papaya ( Carica papaya ) pulp and seed extracts as a potential natural treatment for bacterial stress

Journal of Food Biochemistry, 2019

The current study evaluated the immunomodulatory effects of Carica papaya pulp and seeds methanol... more The current study evaluated the immunomodulatory effects of Carica papaya pulp and seeds methanol (MeOH) extracts on mice infected with Listeria monocytogenes. Gas chromatography-mass spectrometry analysis identified 10 active constituents in C. papaya seed MeOH extract and 10 compounds in C. papaya pulp MeOH extract. The experimental animals were divided into negative control (G1) group, positive control (G2) group, pulp extract treated (G3) group, and seed extract treated (G4) group. After infection of animals (G2, G3, and G4), treatments were started for 3 weeks. Estimation of the immunological parameters showed a marked decrease in IgM levels and an increase in IgG levels in the treated groups (G3 and G4) compared with those in G2. The proinflammatory cytokines (IL-10, IL-12, IL-1β, IL-6, and TGF-β1) were decreased in the treated groups (G3 and G4) compared with those in G2. Nitric oxide levels were also decreased, and the percentages of phagocytosis increased compared with those of G2. The results demonstrated the immunomodulatory and anti-inflammatory effects of C. papaya pulp and seeds MeOH extracts. PRACTICAL APPLICATIONS: Based on the antioxidant and antibacterial activities exhibited by the pulp and seed MeOH extracts investigated in this study, Carica papaya might be considered as a natural source of phytochemicals that could be utilized in novel foods and pharmaceuticals. Further investigation are needed to identify and purify compounds that might be responsible for the observed effects.