ramovatar meena | Jawaharlal Nehru University (original) (raw)

Papers by ramovatar meena

Research paper thumbnail of Bioaccumulation of CdSe Quantum Dots Show Biochemical and Oxidative Damage in Wistar Rats

Oxidative Medicine and Cellular Longevity

Cadmium selenium quantum dots (CdSe QDs) with modified surfaces exhibit superior dispersion stabi... more Cadmium selenium quantum dots (CdSe QDs) with modified surfaces exhibit superior dispersion stability and high fluorescence yield, making them desirable biological probes. The knowledge of cellular and biochemical toxicity has been lacking, and there is little information on the correlation between in vitro and in vivo data. The current study was carried out to assess the toxicity of CdSe QDs after intravenous injection in Wistar male rats (230 g). The rats were given a single dose of QDs of 10, 20, 40, and 80 mg/kg and were kept for 30 days. Following that, various biochemical assays, hematological parameters, and bioaccumulation studies were carried out. Functional as well as clinically significant changes were observed. There was a significant increase in WBC while the RBC decreased. This suggested that CdSe quantum dots had inflammatory effects on the treated rats. The various biochemical assays clearly showed that high dose induced hepatic injury. At a dose of 80 mg/kg, bioaccu...

Research paper thumbnail of Synthesis and Spectral Characterisation of Fabricated Cerium-Doped Magnesium Oxide Nanoparticles: Evaluation of the Antimicrobial Potential and Its Membranolytic Activity through Large Unilamellar Vesicles

Journal of Functional Biomaterials

Considerable attention has been given to Magnesium oxide nanoparticles lately due to their antimi... more Considerable attention has been given to Magnesium oxide nanoparticles lately due to their antimicrobial potential, low toxicity to humans, high thermal stability, biocompatibility, and low cost of production. However, their successful transformation into sustainable drugs is limited due to their low membrane permeability, which reduces their bioavailability in target cells. Herein we propose Cerium-doped magnesium oxide nanoparticles (MgOCeNPs) as a powerful solution to above mentioned limitations and are compared with MgO NPs for their membrane permeability and antimicrobial activity. Both pure and Ce-doped were characterized by various spectroscopic and microscopic techniques, in which an X-ray diffraction (XRD) examination reveals the lattice patterns for doped nanoparticles. Furthermore, Atomic Force Microscopy (AFM) revealed the three-dimensional (3D) structure and height of the nanoparticle. The crystal structure (FCC) of MgO did not change with Ce doping. However, microstruc...

Research paper thumbnail of Untargeted Metabolomics in Piper betle Leaf Extracts to Discriminate the Cultivars of Coastal Odisha, India

Applied Biochemistry and Biotechnology, 2022

Betel leaf is consumed as a mouth freshener due to its characteristic flavor, aromaticity, and me... more Betel leaf is consumed as a mouth freshener due to its characteristic flavor, aromaticity, and medicinal values. Abundance of phytochemicals in betel leaf contributes towards unique qualitative features. Screening of metabolites is quintessential for identifying flavoring betel leaves and their origin. Metabolomics presently lays emphasis on the cumulative application of gas chromatography–mass spectrometry and nuclear magnetic resonance spectroscopic approaches. Here we adopted different protocols based on the above-mentioned analytical metabolomics platform for untargeted plant metabolite profiling followed by multivariate analysis methods and a phytochemical characterization of Piper betel leaf cultivars endemic to coastal Odisha, India. Based on variation in the solvent composition, concentration of solvent, extraction temperature, and incubation periods, five extraction methods were followed in GC–MS and NMR spectroscopy of betel leaf extracts. Phytochemical similarities and differences among the species were characterized through multivariate analysis approaches. Principal component analysis, based on the relative abundance of phytochemicals, indicated that the betel cultivars could be grouped into three groups. Our results of FTIR-, GC–MS-, and NMR-based profiling combined with multivariate analyses suggest that untargeted metabolomics can play a crucial role in documenting metabolic signatures of endemic betel leaf varieties.

Research paper thumbnail of Analyses of the immune-cell reaction and activation mechanisms in the tumor tissue after boron neutron capture therapy

Treatment: L-BPA and fructose were dissolved in disstileed water at a molar ratio of 1:1.5, and t... more Treatment: L-BPA and fructose were dissolved in disstileed water at a molar ratio of 1:1.5, and then a 1.15 M ratio of 1 N NaOH was added. The mixture was stirred until L-BPA had completely dissolved, and the pH value was titrated to 7.6 with 1 N HCl. the solution was filtered through a 0.22 μm syringe filter for sterilization (Merck Millipore, Massachusetts, USA). The final concentration was set to 30 mg/mL. Tumor volumes were calculated using the formula π/6 x Length x Width x Height. Treatments were started when the tumor reached a volume of 100-150 mm3 in the B16-Luc model and 150-220 mm3 in the SCC-VII model. For BNCT group, 500 mg/kg of BPA was subcutaneously injected into mice into the nuchal sites. Tumor was irradiated by thermal and epi-thermal neutron (5MW, Institutes for Integrated Radiation and Nuclear Science, Kyoto University) 45 min after BPA injection for 15 min. For in vivo depletion of CD8+ T cells, CD8+ cell-depleting antibody was injected intraperitoneally(i.p.) ...

Research paper thumbnail of Mineral Uptake and elemental accumulation profiling in Piper betle L. leaves of Coastal Odisha Region

Elemental analysis of soils and leafs is widely employed to quantify the fertilizer and nutrients... more Elemental analysis of soils and leafs is widely employed to quantify the fertilizer and nutrients imbalance pertaining to deficit in agricultural productivity. EDXRF methodology offers an ideal technique to evaluate the direct detection of dried powdered leaf samples. This strategy enabled the concurrent determination of Mg2+, Ca 2+, P-3, K+, Fe2+, Cu2+, B-1, Si+1, Al+3, Ti+4, Mn+4 etc. This present study discriminates depositional behavior of macro minerals like P, S leaf varieties of Bali, Chandrakana and Jhanji. However, Chandrakana variety promotes highest micro mineral accumulation and Jhanji one the least. Vineyard sandy soils of BLS and BUS impart more MnO as compared to clayey soils of PLS and PUS. It also reflects high abundance of Cl in clayey soils relative to sandy soils. Thus, the present findings promote EDXRF employment for direct detection of agricultural samples. The outcomes also motivate further studies for trialing other crops for nutrition and deficiency analysis.

Research paper thumbnail of Preservation of wheat and barley germplasm under natural storage conditions

Research paper thumbnail of Mineralogical composition and C/N contents in soil and water among betel vineyards of coastal Odisha, India

Research paper thumbnail of Modulatory effects of Punica granatum L juice against 2115 MHz (3G) radiation-induced reproductive toxicity in male Wistar rat

Environmental Science and Pollution Research, 2021

Advancements in telecommunication sector result in increasing exposure to electromagnetic (EM) ra... more Advancements in telecommunication sector result in increasing exposure to electromagnetic (EM) radiation, which has been correlated with incidence of male infertility. Therefore, the present study focused on analyzing the consequence of EM radiation (2115 MHz) exposure on the reproductive system of male Wistar rats. Besides, the antioxidant protective effect of Punica granatum juice was also evaluated. For experimental analysis, rats were divided into five groups (control, sham exposed, exposed, herbal plus exposed, and herbal only). Individual group consisted of 6 rats which were exposed to radiation for 45 days (2 h/day). The herbal-treated groups were given 1 ml of Punica granatum extract orally. Various parameters such as organ to body ratio, sperm count, motility, viability, and testis histopathology were studied. Furthermore, oxidative stress parameters and free radical generation were analyzed. The exposed group showed changes in sperm parameters along with decrease in seminiferous tubule diameter. On the contrary, herbal-exposed group showed enhanced sperm count, increased motility, and viability in comparison to exposed group. Histopathology studies also revealed the protective role of herbal juice. Significant alteration in oxidative parameters along with an enhanced free radical generation in exposed group and reduction in herbal groups was observed. The results thus indicate that continuous exposure to EM radiation can lead to oxidative stress which induces biochemical changes in rat sperms. However, Punica granatum extract has a protective role against oxidative damage induced by EM radiation.

Research paper thumbnail of Tailoring of Structural, Morphological and Optical Properties of Boron Nitride/Nickel Oxide (BN100-x/NiOx) Nanocomposites

Journal of Cluster Science, 2020

Research paper thumbnail of Boron Neutron Capture Therapy Study of 10B Enriched Nanostructured Boron Carbide Against Cervical Cancer and Glioblastoma Cell Line

Journal of Cluster Science, 2020

Research paper thumbnail of Emerging Antineoplastic Plant-Based Gold Nanoparticle Synthesis: A Mechanistic Exploration of their Anticancer Activity Toward Cervical Cancer Cells

Journal of Cluster Science, 2019

In this work, gold nanoparticles (AuNPs) were synthesised using the fresh leaves extract of Ponga... more In this work, gold nanoparticles (AuNPs) were synthesised using the fresh leaves extract of Pongamia pinnata (PLE) and characterized using various analytical techniques such as UV–visible spectroscopy, TEM, SEM, EDX, and XRD. UV–Visible spectra showed surface plasmon resonance (SPR) peaks in the range of 520–540 nm signifying the synthesis of colloidal AuNPs stabilised by PLE. Further, we investigated the cytotoxic effects of AuNPs against human cervical cancer cell line (HeLa). MTT assay screened IC50 value of 200 μg/mL at 24 h for AuNPs formed at varying HAuCl4 concentration. Flow cytometry measurements using H2DCFDA showed that the toxicity of AuNPs was attributed to the generation of reactive oxygen species (ROS). Besides, the in vitro anticancer activities of AuNPs were studied in HeLa cells by several assays which resulted in altered cell morphology, reduced potential of wound healing, inactive mitochondria due to loss of mitochondrial membrane potential (MMP) and cell cycle arrest. AuNPs showed dose-dependent selective toxicity towards HeLa cells and were non-toxic against human embryonic kidney cell line (HEK293). The study revealed an efficient, ecofriendly and simple method for synthesis of multifunctional AuNPs using green synthetic approach with high anticancer potential for cervical cancer cell line.

Research paper thumbnail of Boron nitride (10BN) a prospective material for treatment of cancer by boron neutron capture therapy (BNCT)

Research paper thumbnail of Piper betle: Augmented Synthesis of Gold Nanoparticles and Its In-vitro Cytotoxicity Assessment on HeLa and HEK293 Cells

Journal of Cluster Science, 2019

Research paper thumbnail of Penicillium Family as Emerging Nanofactory for Biosynthesis of Green Nanomaterials: A Journey into the World of Microorganisms

Journal of Cluster Science, 2019

Research paper thumbnail of Nanostructured boron carbide (B4C): A bio-compatible and recyclable photo-catalyst for efficient wastewater treatment

Research paper thumbnail of New N-benzhydrylpiperazine/1,3,4-oxadiazoles conjugates inhibit the proliferation, migration, and induce apoptosis in HeLa cancer cells via oxidative stress-mediated mitochondrial pathway

Journal of cellular biochemistry, Jan 11, 2018

N-benzhydrylpiperazine and 1,3,4-oxadiazoles are pharmacologically active scaffolds which exhibit... more N-benzhydrylpiperazine and 1,3,4-oxadiazoles are pharmacologically active scaffolds which exhibits significant inhibitory growth effects against various cancer cells, however, antiproliferation effects and the underlying mechanism for inducing apoptosis for aforementioned scaffolds addressing HeLa cancer cells remains uncertain. In this study, N-benzhydrylpiperazine clubbed with 1,3,4-oxadiazoles (4a-4h) were synthesized, subsequently characterized using high resolution spectroscopic techniques and eventually evaluated for their antiproliferation potential by inducing apoptosis in HeLa cancer cells. The MTT assay screening results revealed that among all, compound 4d ( N-benzhydryl-4-((5-(4-aminophenyl)-1,3,4-oxadiazol-2-yl)methyl)piperazine) in particular, exhibited IC value of 28.13 ± 0.21 μg/mL and significantly inhibited the proliferation of HeLa cancer cells in concentration-dependent manner. The in vitro anticancer assays for treated HeLa cells resulted in alterations in the c...

Research paper thumbnail of Nanostructured BN–TiO2 composite with ultra-high photocatalytic activity

New Journal of Chemistry, 2017

Boron nitride and titanium oxide composite (BN–TiO2) photocatalyst endowed with high specific sur... more Boron nitride and titanium oxide composite (BN–TiO2) photocatalyst endowed with high specific surface area and large pore size was synthesized by ice bath method.

Research paper thumbnail of PLGA-CTAB curcumin nanoparticles: Fabrication, characterization and molecular basis of anticancer activity in triple negative breast cancer cell lines (MDA-MB-231 cells)

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 11, 2017

Triple-negative breast cancers (TNBC) are aggressive cancers, which do not control by hormonal th... more Triple-negative breast cancers (TNBC) are aggressive cancers, which do not control by hormonal therapy or therapies that target HER-2 receptors. Curcumin (Cur) has shown cytotoxic effects in multiple cancer cell lines. However, its medical uses remain limited due to low aqueous solubility and poor bioavailability. Therefore, present study was aimed to fabricate the small positive charge curcumin nanoparticles (CN) by nanoprecipitation methods using PLGA and CTAB, and to evaluate its anticancer efficacy and underlying the mechanism in triple negative breast cancer cell lines (MDA-MB-231 cells). In in-vitro drug release assay, Cur was released from CN by flicking diffusion and anomalous transport process. CN showed a higher cellular incorporation than free Cur resulted in higher cytotoxicity. Checking the anticancer activity at the molecular level, Cur has shown to induce the reactive oxygen species production that subsequently causes the DNA damage and resulting in p38-MAPK activatio...

Research paper thumbnail of Nanostructured Boron Nitride With High Water Dispersibility For Boron Neutron Capture Therapy

Scientific reports, Oct 19, 2016

Highly water dispersible boron based compounds are innovative and advanced materials which can be... more Highly water dispersible boron based compounds are innovative and advanced materials which can be used in Boron Neutron Capture Therapy for cancer treatment (BNCT). Present study deals with the synthesis of highly water dispersible nanostructured Boron Nitride (BN). Unique and relatively low temperature synthesis route is the soul of present study. The morphological examinations (Scanning/transmission electron microscopy) of synthesized nanostructures showed that they are in transient phase from two dimensional hexagonal sheets to nanotubes. It is also supported by dual energy band gap of these materials calculated from UV- visible spectrum of the material. The theoretically calculated band gap also supports the same (calculated by virtual nano lab Software). X-ray diffraction (XRD) analysis shows that the synthesized material has deformed structure which is further supported by Raman spectroscopy. The structural aspect of high water disperse ability of BN is also studied. The ultra...

Research paper thumbnail of Fabrication of BSA-green tea polyphenols-chitosan nanoparticles and its role in radioprotection: A molecular and biochemical approach

Journal of agricultural and food chemistry, Jan 7, 2016

Normal tissue damage from ionizing radiation during radiotherapy is a major concern in cancer tre... more Normal tissue damage from ionizing radiation during radiotherapy is a major concern in cancer treatment. Tea polyphenols (TPs) have shown to reduce radiation-induced damage in multiple studies, but its pharmacological application is still limited due to poor bioavailability. Present study was aimed to increase TPs bioavailability by nanoformulation using BSA as matrix and chitosan as external shell. Encapsulated TPs nanoparticles were spherical in size and promoted TPs stability in normal and gastrointestinal condition without losing antioxidant activity. Oral administration of nanoparticles for 3 days prior to irradiation exposure has shown to protect mice from haematological injuries that result in reduction of radiation-induced lethality. TPs reduces radiation-induced oxidative damage and apoptosis by restoring the redox status through Nrf2-ERK pathway and reducing Bax expression respectively. Regarding potency, encapsulated TPs have shown a significantly higher level of radiopro...

Research paper thumbnail of Bioaccumulation of CdSe Quantum Dots Show Biochemical and Oxidative Damage in Wistar Rats

Oxidative Medicine and Cellular Longevity

Cadmium selenium quantum dots (CdSe QDs) with modified surfaces exhibit superior dispersion stabi... more Cadmium selenium quantum dots (CdSe QDs) with modified surfaces exhibit superior dispersion stability and high fluorescence yield, making them desirable biological probes. The knowledge of cellular and biochemical toxicity has been lacking, and there is little information on the correlation between in vitro and in vivo data. The current study was carried out to assess the toxicity of CdSe QDs after intravenous injection in Wistar male rats (230 g). The rats were given a single dose of QDs of 10, 20, 40, and 80 mg/kg and were kept for 30 days. Following that, various biochemical assays, hematological parameters, and bioaccumulation studies were carried out. Functional as well as clinically significant changes were observed. There was a significant increase in WBC while the RBC decreased. This suggested that CdSe quantum dots had inflammatory effects on the treated rats. The various biochemical assays clearly showed that high dose induced hepatic injury. At a dose of 80 mg/kg, bioaccu...

Research paper thumbnail of Synthesis and Spectral Characterisation of Fabricated Cerium-Doped Magnesium Oxide Nanoparticles: Evaluation of the Antimicrobial Potential and Its Membranolytic Activity through Large Unilamellar Vesicles

Journal of Functional Biomaterials

Considerable attention has been given to Magnesium oxide nanoparticles lately due to their antimi... more Considerable attention has been given to Magnesium oxide nanoparticles lately due to their antimicrobial potential, low toxicity to humans, high thermal stability, biocompatibility, and low cost of production. However, their successful transformation into sustainable drugs is limited due to their low membrane permeability, which reduces their bioavailability in target cells. Herein we propose Cerium-doped magnesium oxide nanoparticles (MgOCeNPs) as a powerful solution to above mentioned limitations and are compared with MgO NPs for their membrane permeability and antimicrobial activity. Both pure and Ce-doped were characterized by various spectroscopic and microscopic techniques, in which an X-ray diffraction (XRD) examination reveals the lattice patterns for doped nanoparticles. Furthermore, Atomic Force Microscopy (AFM) revealed the three-dimensional (3D) structure and height of the nanoparticle. The crystal structure (FCC) of MgO did not change with Ce doping. However, microstruc...

Research paper thumbnail of Untargeted Metabolomics in Piper betle Leaf Extracts to Discriminate the Cultivars of Coastal Odisha, India

Applied Biochemistry and Biotechnology, 2022

Betel leaf is consumed as a mouth freshener due to its characteristic flavor, aromaticity, and me... more Betel leaf is consumed as a mouth freshener due to its characteristic flavor, aromaticity, and medicinal values. Abundance of phytochemicals in betel leaf contributes towards unique qualitative features. Screening of metabolites is quintessential for identifying flavoring betel leaves and their origin. Metabolomics presently lays emphasis on the cumulative application of gas chromatography–mass spectrometry and nuclear magnetic resonance spectroscopic approaches. Here we adopted different protocols based on the above-mentioned analytical metabolomics platform for untargeted plant metabolite profiling followed by multivariate analysis methods and a phytochemical characterization of Piper betel leaf cultivars endemic to coastal Odisha, India. Based on variation in the solvent composition, concentration of solvent, extraction temperature, and incubation periods, five extraction methods were followed in GC–MS and NMR spectroscopy of betel leaf extracts. Phytochemical similarities and differences among the species were characterized through multivariate analysis approaches. Principal component analysis, based on the relative abundance of phytochemicals, indicated that the betel cultivars could be grouped into three groups. Our results of FTIR-, GC–MS-, and NMR-based profiling combined with multivariate analyses suggest that untargeted metabolomics can play a crucial role in documenting metabolic signatures of endemic betel leaf varieties.

Research paper thumbnail of Analyses of the immune-cell reaction and activation mechanisms in the tumor tissue after boron neutron capture therapy

Treatment: L-BPA and fructose were dissolved in disstileed water at a molar ratio of 1:1.5, and t... more Treatment: L-BPA and fructose were dissolved in disstileed water at a molar ratio of 1:1.5, and then a 1.15 M ratio of 1 N NaOH was added. The mixture was stirred until L-BPA had completely dissolved, and the pH value was titrated to 7.6 with 1 N HCl. the solution was filtered through a 0.22 μm syringe filter for sterilization (Merck Millipore, Massachusetts, USA). The final concentration was set to 30 mg/mL. Tumor volumes were calculated using the formula π/6 x Length x Width x Height. Treatments were started when the tumor reached a volume of 100-150 mm3 in the B16-Luc model and 150-220 mm3 in the SCC-VII model. For BNCT group, 500 mg/kg of BPA was subcutaneously injected into mice into the nuchal sites. Tumor was irradiated by thermal and epi-thermal neutron (5MW, Institutes for Integrated Radiation and Nuclear Science, Kyoto University) 45 min after BPA injection for 15 min. For in vivo depletion of CD8+ T cells, CD8+ cell-depleting antibody was injected intraperitoneally(i.p.) ...

Research paper thumbnail of Mineral Uptake and elemental accumulation profiling in Piper betle L. leaves of Coastal Odisha Region

Elemental analysis of soils and leafs is widely employed to quantify the fertilizer and nutrients... more Elemental analysis of soils and leafs is widely employed to quantify the fertilizer and nutrients imbalance pertaining to deficit in agricultural productivity. EDXRF methodology offers an ideal technique to evaluate the direct detection of dried powdered leaf samples. This strategy enabled the concurrent determination of Mg2+, Ca 2+, P-3, K+, Fe2+, Cu2+, B-1, Si+1, Al+3, Ti+4, Mn+4 etc. This present study discriminates depositional behavior of macro minerals like P, S leaf varieties of Bali, Chandrakana and Jhanji. However, Chandrakana variety promotes highest micro mineral accumulation and Jhanji one the least. Vineyard sandy soils of BLS and BUS impart more MnO as compared to clayey soils of PLS and PUS. It also reflects high abundance of Cl in clayey soils relative to sandy soils. Thus, the present findings promote EDXRF employment for direct detection of agricultural samples. The outcomes also motivate further studies for trialing other crops for nutrition and deficiency analysis.

Research paper thumbnail of Preservation of wheat and barley germplasm under natural storage conditions

Research paper thumbnail of Mineralogical composition and C/N contents in soil and water among betel vineyards of coastal Odisha, India

Research paper thumbnail of Modulatory effects of Punica granatum L juice against 2115 MHz (3G) radiation-induced reproductive toxicity in male Wistar rat

Environmental Science and Pollution Research, 2021

Advancements in telecommunication sector result in increasing exposure to electromagnetic (EM) ra... more Advancements in telecommunication sector result in increasing exposure to electromagnetic (EM) radiation, which has been correlated with incidence of male infertility. Therefore, the present study focused on analyzing the consequence of EM radiation (2115 MHz) exposure on the reproductive system of male Wistar rats. Besides, the antioxidant protective effect of Punica granatum juice was also evaluated. For experimental analysis, rats were divided into five groups (control, sham exposed, exposed, herbal plus exposed, and herbal only). Individual group consisted of 6 rats which were exposed to radiation for 45 days (2 h/day). The herbal-treated groups were given 1 ml of Punica granatum extract orally. Various parameters such as organ to body ratio, sperm count, motility, viability, and testis histopathology were studied. Furthermore, oxidative stress parameters and free radical generation were analyzed. The exposed group showed changes in sperm parameters along with decrease in seminiferous tubule diameter. On the contrary, herbal-exposed group showed enhanced sperm count, increased motility, and viability in comparison to exposed group. Histopathology studies also revealed the protective role of herbal juice. Significant alteration in oxidative parameters along with an enhanced free radical generation in exposed group and reduction in herbal groups was observed. The results thus indicate that continuous exposure to EM radiation can lead to oxidative stress which induces biochemical changes in rat sperms. However, Punica granatum extract has a protective role against oxidative damage induced by EM radiation.

Research paper thumbnail of Tailoring of Structural, Morphological and Optical Properties of Boron Nitride/Nickel Oxide (BN100-x/NiOx) Nanocomposites

Journal of Cluster Science, 2020

Research paper thumbnail of Boron Neutron Capture Therapy Study of 10B Enriched Nanostructured Boron Carbide Against Cervical Cancer and Glioblastoma Cell Line

Journal of Cluster Science, 2020

Research paper thumbnail of Emerging Antineoplastic Plant-Based Gold Nanoparticle Synthesis: A Mechanistic Exploration of their Anticancer Activity Toward Cervical Cancer Cells

Journal of Cluster Science, 2019

In this work, gold nanoparticles (AuNPs) were synthesised using the fresh leaves extract of Ponga... more In this work, gold nanoparticles (AuNPs) were synthesised using the fresh leaves extract of Pongamia pinnata (PLE) and characterized using various analytical techniques such as UV–visible spectroscopy, TEM, SEM, EDX, and XRD. UV–Visible spectra showed surface plasmon resonance (SPR) peaks in the range of 520–540 nm signifying the synthesis of colloidal AuNPs stabilised by PLE. Further, we investigated the cytotoxic effects of AuNPs against human cervical cancer cell line (HeLa). MTT assay screened IC50 value of 200 μg/mL at 24 h for AuNPs formed at varying HAuCl4 concentration. Flow cytometry measurements using H2DCFDA showed that the toxicity of AuNPs was attributed to the generation of reactive oxygen species (ROS). Besides, the in vitro anticancer activities of AuNPs were studied in HeLa cells by several assays which resulted in altered cell morphology, reduced potential of wound healing, inactive mitochondria due to loss of mitochondrial membrane potential (MMP) and cell cycle arrest. AuNPs showed dose-dependent selective toxicity towards HeLa cells and were non-toxic against human embryonic kidney cell line (HEK293). The study revealed an efficient, ecofriendly and simple method for synthesis of multifunctional AuNPs using green synthetic approach with high anticancer potential for cervical cancer cell line.

Research paper thumbnail of Boron nitride (10BN) a prospective material for treatment of cancer by boron neutron capture therapy (BNCT)

Research paper thumbnail of Piper betle: Augmented Synthesis of Gold Nanoparticles and Its In-vitro Cytotoxicity Assessment on HeLa and HEK293 Cells

Journal of Cluster Science, 2019

Research paper thumbnail of Penicillium Family as Emerging Nanofactory for Biosynthesis of Green Nanomaterials: A Journey into the World of Microorganisms

Journal of Cluster Science, 2019

Research paper thumbnail of Nanostructured boron carbide (B4C): A bio-compatible and recyclable photo-catalyst for efficient wastewater treatment

Research paper thumbnail of New N-benzhydrylpiperazine/1,3,4-oxadiazoles conjugates inhibit the proliferation, migration, and induce apoptosis in HeLa cancer cells via oxidative stress-mediated mitochondrial pathway

Journal of cellular biochemistry, Jan 11, 2018

N-benzhydrylpiperazine and 1,3,4-oxadiazoles are pharmacologically active scaffolds which exhibit... more N-benzhydrylpiperazine and 1,3,4-oxadiazoles are pharmacologically active scaffolds which exhibits significant inhibitory growth effects against various cancer cells, however, antiproliferation effects and the underlying mechanism for inducing apoptosis for aforementioned scaffolds addressing HeLa cancer cells remains uncertain. In this study, N-benzhydrylpiperazine clubbed with 1,3,4-oxadiazoles (4a-4h) were synthesized, subsequently characterized using high resolution spectroscopic techniques and eventually evaluated for their antiproliferation potential by inducing apoptosis in HeLa cancer cells. The MTT assay screening results revealed that among all, compound 4d ( N-benzhydryl-4-((5-(4-aminophenyl)-1,3,4-oxadiazol-2-yl)methyl)piperazine) in particular, exhibited IC value of 28.13 ± 0.21 μg/mL and significantly inhibited the proliferation of HeLa cancer cells in concentration-dependent manner. The in vitro anticancer assays for treated HeLa cells resulted in alterations in the c...

Research paper thumbnail of Nanostructured BN–TiO2 composite with ultra-high photocatalytic activity

New Journal of Chemistry, 2017

Boron nitride and titanium oxide composite (BN–TiO2) photocatalyst endowed with high specific sur... more Boron nitride and titanium oxide composite (BN–TiO2) photocatalyst endowed with high specific surface area and large pore size was synthesized by ice bath method.

Research paper thumbnail of PLGA-CTAB curcumin nanoparticles: Fabrication, characterization and molecular basis of anticancer activity in triple negative breast cancer cell lines (MDA-MB-231 cells)

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, Jan 11, 2017

Triple-negative breast cancers (TNBC) are aggressive cancers, which do not control by hormonal th... more Triple-negative breast cancers (TNBC) are aggressive cancers, which do not control by hormonal therapy or therapies that target HER-2 receptors. Curcumin (Cur) has shown cytotoxic effects in multiple cancer cell lines. However, its medical uses remain limited due to low aqueous solubility and poor bioavailability. Therefore, present study was aimed to fabricate the small positive charge curcumin nanoparticles (CN) by nanoprecipitation methods using PLGA and CTAB, and to evaluate its anticancer efficacy and underlying the mechanism in triple negative breast cancer cell lines (MDA-MB-231 cells). In in-vitro drug release assay, Cur was released from CN by flicking diffusion and anomalous transport process. CN showed a higher cellular incorporation than free Cur resulted in higher cytotoxicity. Checking the anticancer activity at the molecular level, Cur has shown to induce the reactive oxygen species production that subsequently causes the DNA damage and resulting in p38-MAPK activatio...

Research paper thumbnail of Nanostructured Boron Nitride With High Water Dispersibility For Boron Neutron Capture Therapy

Scientific reports, Oct 19, 2016

Highly water dispersible boron based compounds are innovative and advanced materials which can be... more Highly water dispersible boron based compounds are innovative and advanced materials which can be used in Boron Neutron Capture Therapy for cancer treatment (BNCT). Present study deals with the synthesis of highly water dispersible nanostructured Boron Nitride (BN). Unique and relatively low temperature synthesis route is the soul of present study. The morphological examinations (Scanning/transmission electron microscopy) of synthesized nanostructures showed that they are in transient phase from two dimensional hexagonal sheets to nanotubes. It is also supported by dual energy band gap of these materials calculated from UV- visible spectrum of the material. The theoretically calculated band gap also supports the same (calculated by virtual nano lab Software). X-ray diffraction (XRD) analysis shows that the synthesized material has deformed structure which is further supported by Raman spectroscopy. The structural aspect of high water disperse ability of BN is also studied. The ultra...

Research paper thumbnail of Fabrication of BSA-green tea polyphenols-chitosan nanoparticles and its role in radioprotection: A molecular and biochemical approach

Journal of agricultural and food chemistry, Jan 7, 2016

Normal tissue damage from ionizing radiation during radiotherapy is a major concern in cancer tre... more Normal tissue damage from ionizing radiation during radiotherapy is a major concern in cancer treatment. Tea polyphenols (TPs) have shown to reduce radiation-induced damage in multiple studies, but its pharmacological application is still limited due to poor bioavailability. Present study was aimed to increase TPs bioavailability by nanoformulation using BSA as matrix and chitosan as external shell. Encapsulated TPs nanoparticles were spherical in size and promoted TPs stability in normal and gastrointestinal condition without losing antioxidant activity. Oral administration of nanoparticles for 3 days prior to irradiation exposure has shown to protect mice from haematological injuries that result in reduction of radiation-induced lethality. TPs reduces radiation-induced oxidative damage and apoptosis by restoring the redox status through Nrf2-ERK pathway and reducing Bax expression respectively. Regarding potency, encapsulated TPs have shown a significantly higher level of radiopro...