Jubie Selvaraj | JSS College of pharmacy, ooty (original) (raw)

Papers by Jubie Selvaraj

Journal of Natural Remedies

The present study was conducted because of the recent scenario of this pandemic coronavirus outbr... more The present study was conducted because of the recent scenario of this pandemic coronavirus outbreak worldwide. Currently, this disease cannot be treated through specific vaccines and therapeutic medicines. While many vaccines are being investigated, it would take some time for these to be accessible to the masses. Eventual evidence indicates that many COVID-19 patients may die from an irregular release of cytokines called as Cytokine Release Syndrome (CRS) due to the excessive reaction of their immune systems. In worsening patients with COVID-19, CRS played a significant role, from pneumonia via ARDS to cumulative systemic inflammation and eventually to a failing of the multi-system organ. In COVID-19 individuals, a large number of cytokines, including IL-6, IL-1, IL-2, IL-10, TNF- ?, and IFN- ?, participate in the ‘cytokine storm,’ but IL-6, whose higher serum levels are associated with respiratory failure, ARDS, and adverse clinical outcomes, tends to be a critical factor. In Chi...

Machine Learning and Systems Biology in Genomics and Health, 2022

Mini-Reviews in Medicinal Chemistry, 2020

The article has been withdrawn at the request of the editor of the journal Mini-Reviews in Medici... more The article has been withdrawn at the request of the editor of the journal Mini-Reviews in Medicinal Chemistry due to incoherent content. Bentham Science apologizes to the readers of the journal for any inconvenience this may have caused. The Bentham Editorial Policy on Article Withdrawal can be found at https://benthamscience.com/editorial-policies-main.php Bentham Science Disclaimer: It is a condition of publication that manuscripts submitted to this journal have not been published and will not be simultaneously submitted or published elsewhere. Furthermore, any data, illustration, structure or table that has been published elsewhere must be reported, and copyright permission for reproduction must be obtained. Plagiarism is strictly forbidden, and by submitting the article for publication the authors agree that the publishers have the legal right to take appropriate action against the authors, if plagiarism or fabricated information is discovered. By submitting a manuscript, the a...

Fatty Acids, 2017

Recent research supports the beneficial effects of dietary polyunsaturated fatty acids (PUFAs) on... more Recent research supports the beneficial effects of dietary polyunsaturated fatty acids (PUFAs) on inhibiting tumour development. Long-chain fatty acids modulate the tumour cell response to chemotherapeutic drugs. Investigators recently claimed high dietary intake of omega-6 polyunsaturated fatty acids such as linoleic acid especially in association with a low intake of omega-3 polyunsaturated fatty acids such as docosahexaenoic acid to increase risks for cancers of the breast, colon and possibly prostate. In addition to these facts, a number of investigations have demonstrated that a modified fatty acid analogues are promising molecules in cancer prevention and have potential in the treatment of cancer. Although billions of dollars have been spent on research and development on anticancer drugs, the disease remains uncontrolled. It is expected that anticancer agents preferentially kill tumour cells without causing adverse effects on normal cells. But this is rarely achieved with the existing cancer therapy. Hence, polyunsaturated fatty acids have come under the category of nutraceuticals/functional foods; their exploration in the treatment of cancer may be considered as safe. This chapter describes the effects of long-chain fatty acids and their analogues in cancer chemotherapy.

Current Nanomedicine

Background: Tobacco smoking is a major factor leading to cardiovascular diseases. About 48% of ca... more Background: Tobacco smoking is a major factor leading to cardiovascular diseases. About 48% of cardiovascular diseases occur due to cigarette smoking. Bupropion Hydrochloride is non-nicotine treatment for smoking cessation. The existing marketed formulation of bupropion have limitations like low bioavailability and extensive first-pass metabolism. In order to boost the bioavailability and increase the brain biodistribution of the drug, a colloidal drug delivery system like nanostructured lipid carriers is employed. Methods: NLC formulation was prepared using microemulsion technique and optimized formula was developed using three-level factorial design. Results: The particle size of the optimized formulation was 162 nm, Polydispersity index was 12.2% and zeta potential was -29.0mV. Entrapment efficiency was found to be 41.2%. SEM images show that these NLCs are spherical. In-vitro drug release study was conducted and at the end of 72 hours, 50 % of drug was released, indicates the su...

International Journal of Applied Pharmaceutics

Objective: Ovarian cancer is the most deadly cancer in women, ranking fourth among all fatal dise... more Objective: Ovarian cancer is the most deadly cancer in women, ranking fourth among all fatal diseases in women. Conventional chemotherapy has its own plethora of challenges, such as side effects and disease relapse. Hydroxyurea is a type of anticancer drug that is commonly used to treat malignancies. This study aims to develop and optimize hydroxyurea nanostructured lipid carriers (NLCs) to improve the therapeutic index and reduce its side effects in the effective treatment of OC. Methods: NLCs were prepared by microemulsion technique. They were prepared and optimized using the design of experiment for particle size and drug entrapment efficiency. Particle size, polydispersity index, zeta potential, morphology, in vitro release, and stability were all examined in the optimized formulation. Results: The results showed that the particle size of the NLCs was in the range of 224 nm to 634 nm. The drug entrapment efficiency of the NLCs was in the range of 46.33 % to 70.43 %. The optimize...

RSC Advances

Structure-based insilico-guided screening of potential polytargeting anti-COVID phytochemical: ga... more Structure-based insilico-guided screening of potential polytargeting anti-COVID phytochemical: gallocatechin gallate analogue.

Bioinformation, 2021

The enzyme, α-topoisomerase II (α-Topo II), is known to regulate efficiently the topology of DNA.... more The enzyme, α-topoisomerase II (α-Topo II), is known to regulate efficiently the topology of DNA. It is highly expressed in rapidly proliferating cells and plays an important role in replication, transcription and chromosome organisation. This has prompted several investigators to pursue α-Topo II inhibitors as anticancer agents. δ-Carboline, a natural product, and its synthetic derivatives are known to exert potent anticancer activity by selectively targeting α-Topo II. Therefore, it is of interest to design carboline derivatives fused with pyrrolidine-2,5-dione in this context. δ-Carbolines fused with pyrrolidine-2,5-dione are of interest because the succinimide part of fused heteroaromatic molecule can interact with the ATP binding pocket via the hydrogen bond network with selectivity towards α-Topo II. The 300 derivatives designed were subjected to the Lipinski rule of 5, ADMET and toxicity prediction. The designed compounds were further analysed using molecular docking analysis...

Journal of Natural Remedies, 2021

Context: The novel coronavirus named as COVID-19 (SARS-CoV-2) from its origin in Hubei spread acr... more Context: The novel coronavirus named as COVID-19 (SARS-CoV-2) from its origin in Hubei spread across the continent in a short period of six monthsâ€™ time. Till date there is no drug to cure the novel corona virus SARS-CoV-2. Earlier studies on SARS-CoV-1 suggests that interleukin 6 (IL-6) and Interleukin 8 (IL-8) were in the higher levels indicating the key role of IL. Aim: Molecular simulation studies were carried out on the selected 24 chemical constituents present in rasam against IL-6 to identify the key interaction between the amino acid residues and their chemical structure.Â Materials and Methods: A library of 24 chemical constituents was sketched using Chem Sketch programming 8.0. The 3D structures of ligands were retrieved in mol format in Maestro v 11.3 and the ligands were optimized utilizing ligprep (4.3) module (SchrÃ¶dinger 2018-1). Results: One of the chemical constituents sinigrin a glucosinolate emerged as top scorer with a GLIDE score of -6.333. It was apparent fr...

International Journal of Peptide Research and Therapeutics, 2022

Current Drug Research Reviews, 2022

: New drug development for a disease is a tedious, time-consuming, complex, and expensive process... more : New drug development for a disease is a tedious, time-consuming, complex, and expensive process. Even if it is done, the chances for success of newly developed drugs are still very low. Modern reports state that repurposing the pre-existing drugs will have more efficient functioning than newly developed drugs. This repurposing process will save time, reduce expenses and provide more success rate. The only limitation for this repurposing is getting a desired pharmacological and characteristic parameter of various drugs from vast data about medications, their effects, and target mechanisms. This drawback can be avoided by introducing computational methods of analysis. This includes various network analysis types that use various biological processes and relationships with various drugs to simplify data interpretation. Some of the data sets now available in standard, and simplified forms include gene expression, drug-target interactions, protein networks, electronic health records, clinical trial results, and drug adverse event reports. Integrating various data sets and interpretation methods allows a more efficient and easy way to repurpose an exact drug for the desired target and effect. In this review, we are going to discuss briefly various computational biological network analysis methods like gene regulatory networks, metabolic networks, protein-protein interaction networks, drug-target interaction networks, drugdisease association networks, drug-drug interaction networks, drug-side effects networks, integrated network-based methods, semantic link networks, and isoform-isoform networks. Along with this, we briefly discussed the drug's limitations, prediction methodologies, and data sets utilised in various biological networks for drug repurposing.

Current Bioactive Compounds, 2022

Background: Genistein is one of many new cytotoxic drug leads that have been discovered recently.... more Background: Genistein is one of many new cytotoxic drug leads that have been discovered recently. It is a well-studied chemopreventive drug that has attracted the attention of the scientific community due to its favourable effects on grave illnesses in humans such as cancer. Objective: The present study focuses on the methanolic extraction, isolation, and characterization of an isoflavone, genistein, from the weed plant of Cytisus scoparius Linn. (family: Fabaceae). Methods: The phytochemical structure of genistein was confirmed by its spectral and chromatography analysis through IR, HPTLC, LCMS, NMR, etc. The total flavonoid fraction of methanolic extracts containing genistein was evaluated for in-vitro cytotoxic activity on cervical cancer HeLa cells using MTT assay, cell cycle analysis, and topoisomerase II inhibitory activity. Results: The result of the in-vitro cytotoxicity study showed the IC50 value of 4.5 μg/ml, which shows that the isolated compound is cytotoxic. The result...

Adenocarpus. mannii species belongs to family Fabaceae, well represented in south west region and... more Adenocarpus. mannii species belongs to family Fabaceae, well represented in south west region and west region of cameroon. Since, the cytotxic results of extracts and isolated compounds of A. mannii on human colon cancer cell lines are promising, it is decided to study their in silico binding studies towards the target protein involved. The present study focuses the in-silico docking studies of four flavonoids and one triterpene isolated from Adenocarpus mannii using Discovery Studio 4.1 Client. All the compounds exhibited a good interaction within the targeted domain compared to standards drugs. The compounds Isovitixin and chrysin 7-O-β-D-glucopyranoside show good docking properties with the highest activation energy of -1.14034 kcal/mol and -1.91612 kcal/mol respectively. Their physicochemical properties are also in accepatable range. These results suggest that the identified compounds can act as potential ‘leads” to inhibit colon cancer cells.

The family Ericaceae is a large cosmopolitan family, represented by 124 genus and 4100 species. S... more The family Ericaceae is a large cosmopolitan family, represented by 124 genus and 4100 species. She predominates in the temperate regions and tropical mountains of extra South East, Asia and America. The Ericaceae family produces many edible berries that can be grown, but others are violent poisons and ornamental plants. Traditional pharmacopoeias used these plant families to cure many diseases such as antidiarrheal, anti-inflammatory, urinary astringent and antiseptic activities. The species of Agauriasalicifolia belongs to the genus Agauria. Agauriasalicifolia is a species of Central Africa, Southern Africa and Madagascar. In Cameroon, this species is especially found in KilumIjim (Oku) and the South West region particularly in the Bamboutos Mountain. A. salicifolia are used for the treatment of several diseases such as bites by snakes, skin diseases, on the wounds caused by scarification, rheumatism, sexually transmitted diseases, insecticide, fungal infections, scabies. The leav...

Journal of Ethnopharmacology, 2021

ETHNOPHARMACOLOGICAL RELEVANCE Chandamarutha Chenduram (CC), an Indian traditional Siddha prepara... more ETHNOPHARMACOLOGICAL RELEVANCE Chandamarutha Chenduram (CC), an Indian traditional Siddha preparation officially recorded in the Siddha formulary of India and its composition are widely used in the Siddha practice of neurological disorders like stroke/paralysis in India. However, the scientific validation and mechanistic evidence is lacking and yet to be elucidated. AIM OF THE STUDY To establish the scientific evidences and to explore the possible neuroprotective mechanism of CC in cerebral ischemia. MATERIALS AND METHODS Chemical standardization of the CC was performed using atomic absorption spectroscopy and gravimetric analysis. Acute toxicity study for CC in mice was performed in accordance with OECD 423 guidelines. CC (5 mg/kg) and CC (10 mg/kg) were investigated in bilateral common carotid occlusion (BCCAo) model in mice. After, behavioral assessments, the brain samples were collected and the hippocampus region was micro-dissected for neurotransmitter, neurobiochemicals and inflammatory cytokines estimation. The excitatory amino acid transporter-2 (EAAT-2) expressions was analyzed by RT-PCR to understand the possible molecular mechanism. In addition, hematoxylin and eosin staining of CA1 hippocampal brain region was performed to support the neuroprotective effect of CC in ischemic condition. RESULTS Chemical standardization analysis showed that CC has acceptable range of mercury (0.82 ppm) and elemental sulphur (11% w/w). Also, other heavy metal limits were found to be less or not detectable. Toxicity study also evidenced the safety profile of CC. CC has significantly reversed the behavioral dysfunctions (p < 0.001) in global ischemic mice. Treatment with CC has attenuated the excitatory neurotransmitter glutamate, lipid peroxide, nitric oxide, cytokines (IL-1β, TNF-α) (p < 0.001) and increased the antioxidant enzymes (SOD, CAT, GSH) and EAAT-2 expression level (p < 0.001) in ischemic brain. The hematoxylin and eosin staining in CA1 region of hippocampus also evidence the neuroprotective effect exhibited by CC. CONCLUSIONS Treatment with CC has exhibited dose dependent effect and CC10 has shown significant protective effect in comparison to CC5 in most of the parameters studied. CC prevented further degeneration of neurons in cerebral ischemic mice through ameliorating inflammatory cytokines and oxy-radicals mediated EAAT-2 dysfunction and subsequent excitotoxicity in neurons.

Advanced Pharmaceutical Bulletin, 2021

The concept of immunomodulation was proposed by Edward Jenner, while working on polio vaccine in ... more The concept of immunomodulation was proposed by Edward Jenner, while working on polio vaccine in 1796. Many of the autoimmune diseases such as rheumatoid arthritis, inflammatory bowel diseases, psoriatic arthritis and system lupus erythematosus, viral diseases and, some cancers are characterized with elevated levels of "immunocytokine" gene expression, including, tumor necrosis factor-α, various interleukins, cytotoxic T-cell antigen-4, B-cell activating factor. For the treatment of these diseases, the immunologically-based therapies play the major role. In these lines, the usage of phytomedicines as immunostimulants /immunosuppressants have been enhanced considerably in last few decades and also used as a prophylactic treatment for various ailments. Phytochemicals such as flavonoids, terpenoids, polysaccharides, lactones, alkaloids, glycosides and saponins present in several plants, have been confirmed to exhibit immunomodulating properties. This review focuses on the tra...

Mini-Reviews in Medicinal Chemistry, 2022

: Many flaviviruses are remarkable human pathogens that can be transmitted by mosquitoes and tick... more : Many flaviviruses are remarkable human pathogens that can be transmitted by mosquitoes and ticks. Despite the availability of vaccines for viral infections such as yellow fever, Japanese encephalitis, and tick-borne encephalitis, flavivirus-like dengue is still a significant life-threatening illness worldwide. To date, there is no antiviral treatment for dengue therapy. Industry and the research community have been taking ongoing steps to improve anti-flavivirus treatment to meet this clinical need. The successful activity has been involved in the inhibition of the virus entry fusion process in the last two decades. In this study, the latest understanding of the use of small molecules used as fusion inhibitors has been comprehensively presented. We summarized the structure, the process of fusion of dengue virus E protein (DENV E), and the amino acids involved in the fusion process. Special attention has been given to small molecules that allow conformational changes to DENV E protein, viz. blocking the pocket of βOG, which is important for fusion.

Mini-Reviews in Medicinal Chemistry, 2021

: Vitamin-D deficiency is a global concern. Gene mutations in the vitamin D receptor’s (VDR) liga... more : Vitamin-D deficiency is a global concern. Gene mutations in the vitamin D receptor’s (VDR) ligand binding domain (LBD) variously alter the ligand binding affinity, heterodimerization with retinoid X receptor (RXR) and inhibit coactivator interactions. These LBD mutations may result in partial or total hormone unresponsiveness. A plethora of evidence report that selective long chain polyunsaturated fatty acids (PUFAs) including eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA) and arachidonic acid (AA) bind to the ligand-binding domain of VDR and lead to transcriptional activation. We therefore hypothesize that selective PUFAs would modulate the dynamics and kinetics of VDRs, irrespective bioactive of vitamin-D binding. The spatial arrangements of the selected PUFAs in VDR active site were examined by in-silico docking studies. The docking results revealed that PUFAs have fatty acid structure-specific binding affinity towards VDR. The calculated EPA, DHA &amp;amp; AA binding energies (Cdocker energy) were lesser compared to vitamin-D in wild type of VDR (PDB id: 2ZLC). Of note, the DHA has higher binding interactions to the mutated VDR (PDB id: 3VT7) when compared to the standard Vitamin-D. Molecular dynamic simulation was utilized to confirm the stability of potential compound binding of DHA with mutated VDR complex. These findings suggest the unique roles of PUFAs in VDR activation and may offer alternate strategy to circumvent vitamin-D deficiency.

Current Drug Research Reviews, 2020

Conventional drug discovery is a time consuming and expensive expedition with less clinical prefe... more Conventional drug discovery is a time consuming and expensive expedition with less clinical preference achievement proportion intended for breast cancer therapy. Even if numerous novel approaches to the conformation of drugs have been introduced for breast cancer therapy, they are yet to be implemented in clinical practice. This tempting strategy facilitates a remarkable chance to take the entire benefit of existing drugs. Despite drug repurposing significantly decrease the investigational period and cost, it has got many objections and issues. Scaffold repurposing is an approach that procures a novel significance on the decrepit motto of “to commencement with a pristine drug” . Hence, we move into a probable and nearer approach, the exploitation of scaffolds, which was originally developed for other purposes, including anti-tumor activity. In this review, we summarize different drugs and scaffolds used in breast cancer therapy.