Mahesh Adhikari | Kangwon National University (original) (raw)
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Papers by Mahesh Adhikari
A previously unrecorded species, Metacordyceps chlamydosporia KNU14-22, was isolated from soil in... more A previously unrecorded species, Metacordyceps chlamydosporia KNU14-22, was isolated from soil in Korea. Identification of the fungal species was based on morphological and molecular characteristics. This species has not been previously reported in Korea and herein we present data with detailed descriptions and figures.
Mycobiology, 2015
Three new fungal species of the genus Mortierella, Mortierella zychae, Mortierella ambigua, and M... more Three new fungal species of the genus Mortierella, Mortierella zychae, Mortierella ambigua, and Mortierella indohii, have been reported in Korea. The fungi were encountered during a study on the fungal community of soil samples collected from different locations in Korea. The species were identified based on molecular and morphological analyses. This study presents detailed descriptions of the morphological observations and molecular phylogenetic analysis of these three fungi. All three species were found to be sensitive to triphenyltetrazolium chloride staining. M. zychae demonstrated the highest intensity of mycelial staining, indicating that this species has the highest potential to produce arachidonic acid of the three species. The staining results indicated that the newly recorded species could potentially be useful for arachidonic acid production.
Antimicrobial activities of nano-materials were tested against several plant pathogens. Twelve di... more Antimicrobial activities of nano-materials were tested against several plant pathogens. Twelve different nanomaterials were used to observe the antagonistic activity against three kinds of mold and sixteen different kinds of watermelon fruit rot pathogens (Acidovorax citrulli). According to the results, no antagonism have been found against the pathogen, Cylindrocarpon destructans. However in the case of Pythium ultimum, combination of Brass/Glucose 1,000 ppm confirmed the mycelial growth reduction by 94%. In addition, little effect was found against Rhizoctonia solani by Ag/Glucose 3,000 ppm. The remaining other nano-materials have different antimicrobial effect depending on the strains of A. citrulli. But in the case of lime (Cu/Salt 1,000 ppm) highest antimicrobial activity was observed with 97%. Moreover growth of five different strains of A. citrulli was checked by 99% with the combination of Ag/Glucose 1,000 ppm. 92% reduction of A. citrulli growth was observed with Brass/CaCO 3 3,000 ppm. Tested nano-materials against different plant pathogens in this study showed the antimicrobial activity at the range of 24-70%.
Korean Journal of Medical Mycology
Oidiodendron flavum KNU13-6 was isolated for the first time from field soil in Korea and identifi... more Oidiodendron flavum KNU13-6 was isolated for the first time from field soil in Korea and identified based on the internal transcribed spacer region (ITS) of rDNA and morphological characteristics. Based on phylogenetic analysis of ITS and morphological characteristics, the species has not been previously reported in Korea. Oidiodendron flavum Svilv., Lent. Bakto. belongs to genus Oidiodendron, a cosmopolitian genus whose members can usually be found in a wide range of habitats, including soils, different cellulose substrates (litter, wood pulp, bark, mosses, paper), and occasionally from lichens or from air [1]. Some Oidiodendron species have been reported as ericoid mycorrhizal fungi [2]. Plants in the Ericaceae have a distinctive ericoid mycorrhizal association , which plays important roles in plant growth, nutrient uptake, and soil mineralization [5]. The ability of some ericoid mycorrhizal fungi (Oidiodendron maius) to dissolve Zn oxide has been reported [6]. In addition, ericoi...
Nigeria is the agrarian country in which the majority of people are engaged in agriculture. The m... more Nigeria is the agrarian country in which the majority of people are engaged in agriculture. The main crops of Nigeria are sorghum, millet, soybean, peanut, cotton, yam, palm trees, rice, rubber, cocoa etc. The economy of Nigeria is depending upon the agricultural sector. Only 1% of the total arable land is under irrigation in Nigeria. Instead of having favorable geographical conditions, export of agricultural product is reducing due to the lack of suitable agricultural technology. 70% of the total populations in Nigeria are engaged in agriculture. In addition annual fertilizer requirement is high to overcome the fertilizer requirement to farmers. As Nigeria has diversified climatic zones, different types of crop can be found in different agro-climatic zones. In 2008 Nigeria was the world's number one yam exporting country. In 2008 Nigeria exported 492 million cocoa beans to the world. For the sustainable agriculture development and economic upliftment of Nigeria, some long term ...
Research in Plant Disease, 2015
A new species of Aspergillus niger (KNU12-3-3) has been isolated from insect specimen in Korea. T... more A new species of Aspergillus niger (KNU12-3-3) has been isolated from insect specimen in Korea. This strain was isolated from Pectocera fortunei. The identification was based on the morphological characteristics and ITS analysis. This is the first report of a new species of A. niger isolated from insects in Korea.
Mycobiology, 2015
We report the isolation of a Gongronella butleri species and describe it based on the analysis of... more We report the isolation of a Gongronella butleri species and describe it based on the analysis of the internal transcribed spacer region of rDNA and morphological characteristics. G. butleri has been reported as a high chitosan producer in the literature. This is the first record of G. butleri isolated from crop field soil in Korea.
Mycobiology, 2015
During a survey of fungal species in South Korea, a species of Volutella ciliata was isolated and... more During a survey of fungal species in South Korea, a species of Volutella ciliata was isolated and described based on the analysis of the internal transcribed spacer region of its rDNA and its morphological characteristics. This is the first record of Volutella ciliata isolated from crop field soil in Korea.
Mycobiology, 2015
The present study is the first report on the isolation of Penicillium menonorum from rhizosphere ... more The present study is the first report on the isolation of Penicillium menonorum from rhizosphere soil in Korea and its identification based on morphological characteristics and internal transcribed spacer gene sequence. The fungal isolate was named KNU-3 and was found to exhibit plant growth-promoting (PGP) activity through indole acetic acid (IAA) and siderophore production, as well as P solubilization. KNU-3 produced 9.7 mg/L IAA and solubilized 408 mg of Ca 3 PO 4 /L, and inoculation with the isolate significantly (p < 0.05) increased the dry biomass of cucumber roots (57%) and shoots (52%). Chlorophyll, starch, protein, and P contents were increased by 16%, 45%, 22%, and 14%, respectively, compared to plants grown in uninoculated soil. The fungus also increased soil dehydrogenase (30%) and acid phosphatase (19%) activities. These results demonstrate that the isolate KNU-3 has potential PGP attributes, and therefore it can be considered as a new fungus to enhance soil fertility and promote plant growth. Moreover, the discovery of PGP ability and traits of this fungus will open new aspects of research and investigations. In this study, plant growth promotion by P. menonorum KNU-3 is reported for the first time in Korea after its original description.
Mycobiology, 2013
Three Penicillium species have been isolated from insect specimens in Korea; Penicillium sp., P. ... more Three Penicillium species have been isolated from insect specimens in Korea; Penicillium sp., P. steckii, and P. polonicum. Penicillium sp. (KNU12-3-2) was isolated from Lixus imperessiventris, while P. polonicum (KNU12-1-8) and Penicillium steckii (KNU12-2-9) were isolated from Muljarus japonicas and Meloe proscarabaeus, respectively. The identification was based on the morphological characteristics of the fungi and in internal transcribed spacer analysis. This is the first report on the isolation of these three species of Penicillium from insects in Korea.
Mycobiology, 2014
A new recorded species of Mortierella was recovered during the investigation of fungal communitie... more A new recorded species of Mortierella was recovered during the investigation of fungal communities in soil samples collected from different locations of Gangwon-do, Korea. The species was identified and described as Mortierella alpina on the basis of phylogenetic analysis of internal transcribed spacer sequences and morphological characteristics. This species has not been officially reported from Korea thus far.
Mycobiology, 2014
Pseudallescheria boydii KNU13-2 was isolated from crop field soil and identified by analysis of i... more Pseudallescheria boydii KNU13-2 was isolated from crop field soil and identified by analysis of internal transcribed spacer regions of rDNA and morphological characteristics. In the literature, P. boydii has been mentioned as a human pathogen. This is the first record of P. boydii isolated from crop field soil in Korea.
A new species of Neosartorya was recovered during investigation of the fungal community in soil s... more A new species of Neosartorya was recovered during investigation of the fungal community in soil samples collected
from different locations in Korea; Neosartorya aureola KNU14-7 was isolated for the first time from field soil in Korea and
identified based on the internal transcribed spacer region of rDNA and morphological characteristics. The species has not been officially reported from Korea and we report it here with description and figures
A previously unrecorded species, Metacordyceps chlamydosporia KNU14-22, was isolated from soil in... more A previously unrecorded species, Metacordyceps chlamydosporia KNU14-22, was isolated from soil in Korea. Identification of the fungal species was based on morphological and molecular characteristics. This species has not been previously reported in Korea and herein we present data with detailed descriptions and figures.
Mycobiology, 2015
Three new fungal species of the genus Mortierella, Mortierella zychae, Mortierella ambigua, and M... more Three new fungal species of the genus Mortierella, Mortierella zychae, Mortierella ambigua, and Mortierella indohii, have been reported in Korea. The fungi were encountered during a study on the fungal community of soil samples collected from different locations in Korea. The species were identified based on molecular and morphological analyses. This study presents detailed descriptions of the morphological observations and molecular phylogenetic analysis of these three fungi. All three species were found to be sensitive to triphenyltetrazolium chloride staining. M. zychae demonstrated the highest intensity of mycelial staining, indicating that this species has the highest potential to produce arachidonic acid of the three species. The staining results indicated that the newly recorded species could potentially be useful for arachidonic acid production.
Antimicrobial activities of nano-materials were tested against several plant pathogens. Twelve di... more Antimicrobial activities of nano-materials were tested against several plant pathogens. Twelve different nanomaterials were used to observe the antagonistic activity against three kinds of mold and sixteen different kinds of watermelon fruit rot pathogens (Acidovorax citrulli). According to the results, no antagonism have been found against the pathogen, Cylindrocarpon destructans. However in the case of Pythium ultimum, combination of Brass/Glucose 1,000 ppm confirmed the mycelial growth reduction by 94%. In addition, little effect was found against Rhizoctonia solani by Ag/Glucose 3,000 ppm. The remaining other nano-materials have different antimicrobial effect depending on the strains of A. citrulli. But in the case of lime (Cu/Salt 1,000 ppm) highest antimicrobial activity was observed with 97%. Moreover growth of five different strains of A. citrulli was checked by 99% with the combination of Ag/Glucose 1,000 ppm. 92% reduction of A. citrulli growth was observed with Brass/CaCO 3 3,000 ppm. Tested nano-materials against different plant pathogens in this study showed the antimicrobial activity at the range of 24-70%.
Korean Journal of Medical Mycology
Oidiodendron flavum KNU13-6 was isolated for the first time from field soil in Korea and identifi... more Oidiodendron flavum KNU13-6 was isolated for the first time from field soil in Korea and identified based on the internal transcribed spacer region (ITS) of rDNA and morphological characteristics. Based on phylogenetic analysis of ITS and morphological characteristics, the species has not been previously reported in Korea. Oidiodendron flavum Svilv., Lent. Bakto. belongs to genus Oidiodendron, a cosmopolitian genus whose members can usually be found in a wide range of habitats, including soils, different cellulose substrates (litter, wood pulp, bark, mosses, paper), and occasionally from lichens or from air [1]. Some Oidiodendron species have been reported as ericoid mycorrhizal fungi [2]. Plants in the Ericaceae have a distinctive ericoid mycorrhizal association , which plays important roles in plant growth, nutrient uptake, and soil mineralization [5]. The ability of some ericoid mycorrhizal fungi (Oidiodendron maius) to dissolve Zn oxide has been reported [6]. In addition, ericoi...
Nigeria is the agrarian country in which the majority of people are engaged in agriculture. The m... more Nigeria is the agrarian country in which the majority of people are engaged in agriculture. The main crops of Nigeria are sorghum, millet, soybean, peanut, cotton, yam, palm trees, rice, rubber, cocoa etc. The economy of Nigeria is depending upon the agricultural sector. Only 1% of the total arable land is under irrigation in Nigeria. Instead of having favorable geographical conditions, export of agricultural product is reducing due to the lack of suitable agricultural technology. 70% of the total populations in Nigeria are engaged in agriculture. In addition annual fertilizer requirement is high to overcome the fertilizer requirement to farmers. As Nigeria has diversified climatic zones, different types of crop can be found in different agro-climatic zones. In 2008 Nigeria was the world's number one yam exporting country. In 2008 Nigeria exported 492 million cocoa beans to the world. For the sustainable agriculture development and economic upliftment of Nigeria, some long term ...
Research in Plant Disease, 2015
A new species of Aspergillus niger (KNU12-3-3) has been isolated from insect specimen in Korea. T... more A new species of Aspergillus niger (KNU12-3-3) has been isolated from insect specimen in Korea. This strain was isolated from Pectocera fortunei. The identification was based on the morphological characteristics and ITS analysis. This is the first report of a new species of A. niger isolated from insects in Korea.
Mycobiology, 2015
We report the isolation of a Gongronella butleri species and describe it based on the analysis of... more We report the isolation of a Gongronella butleri species and describe it based on the analysis of the internal transcribed spacer region of rDNA and morphological characteristics. G. butleri has been reported as a high chitosan producer in the literature. This is the first record of G. butleri isolated from crop field soil in Korea.
Mycobiology, 2015
During a survey of fungal species in South Korea, a species of Volutella ciliata was isolated and... more During a survey of fungal species in South Korea, a species of Volutella ciliata was isolated and described based on the analysis of the internal transcribed spacer region of its rDNA and its morphological characteristics. This is the first record of Volutella ciliata isolated from crop field soil in Korea.
Mycobiology, 2015
The present study is the first report on the isolation of Penicillium menonorum from rhizosphere ... more The present study is the first report on the isolation of Penicillium menonorum from rhizosphere soil in Korea and its identification based on morphological characteristics and internal transcribed spacer gene sequence. The fungal isolate was named KNU-3 and was found to exhibit plant growth-promoting (PGP) activity through indole acetic acid (IAA) and siderophore production, as well as P solubilization. KNU-3 produced 9.7 mg/L IAA and solubilized 408 mg of Ca 3 PO 4 /L, and inoculation with the isolate significantly (p < 0.05) increased the dry biomass of cucumber roots (57%) and shoots (52%). Chlorophyll, starch, protein, and P contents were increased by 16%, 45%, 22%, and 14%, respectively, compared to plants grown in uninoculated soil. The fungus also increased soil dehydrogenase (30%) and acid phosphatase (19%) activities. These results demonstrate that the isolate KNU-3 has potential PGP attributes, and therefore it can be considered as a new fungus to enhance soil fertility and promote plant growth. Moreover, the discovery of PGP ability and traits of this fungus will open new aspects of research and investigations. In this study, plant growth promotion by P. menonorum KNU-3 is reported for the first time in Korea after its original description.
Mycobiology, 2013
Three Penicillium species have been isolated from insect specimens in Korea; Penicillium sp., P. ... more Three Penicillium species have been isolated from insect specimens in Korea; Penicillium sp., P. steckii, and P. polonicum. Penicillium sp. (KNU12-3-2) was isolated from Lixus imperessiventris, while P. polonicum (KNU12-1-8) and Penicillium steckii (KNU12-2-9) were isolated from Muljarus japonicas and Meloe proscarabaeus, respectively. The identification was based on the morphological characteristics of the fungi and in internal transcribed spacer analysis. This is the first report on the isolation of these three species of Penicillium from insects in Korea.
Mycobiology, 2014
A new recorded species of Mortierella was recovered during the investigation of fungal communitie... more A new recorded species of Mortierella was recovered during the investigation of fungal communities in soil samples collected from different locations of Gangwon-do, Korea. The species was identified and described as Mortierella alpina on the basis of phylogenetic analysis of internal transcribed spacer sequences and morphological characteristics. This species has not been officially reported from Korea thus far.
Mycobiology, 2014
Pseudallescheria boydii KNU13-2 was isolated from crop field soil and identified by analysis of i... more Pseudallescheria boydii KNU13-2 was isolated from crop field soil and identified by analysis of internal transcribed spacer regions of rDNA and morphological characteristics. In the literature, P. boydii has been mentioned as a human pathogen. This is the first record of P. boydii isolated from crop field soil in Korea.
A new species of Neosartorya was recovered during investigation of the fungal community in soil s... more A new species of Neosartorya was recovered during investigation of the fungal community in soil samples collected
from different locations in Korea; Neosartorya aureola KNU14-7 was isolated for the first time from field soil in Korea and
identified based on the internal transcribed spacer region of rDNA and morphological characteristics. The species has not been officially reported from Korea and we report it here with description and figures